Australian Cultures of Botryosphaeriaceae Held in Queensland and Victoria Plant Pathology Herbaria Revisited
Australasian Plant Pathology https://doi.org/10.1007/s13313-018-0559-7 ORIGINAL PAPER Australian cultures of Botryosphaeriaceae held in Queensland and Victoria plant pathology herbaria revisited Yu Pei Tan1,2 & Roger G. Shivas1,3 & Thomas S. Marney1 & Jacqueline Edwards4,5 & John Dearnaley6 & Fahimeh Jami7 & Treena I. Burgess7,8 Received: 22 January 2018 /Accepted: 24 April 2018 # Australasian Plant Pathology Society Inc. 2018 Abstract The Botryosphaeriaceae is one of the most widespread and cosmopolitan endophytic group of fungi. However, the species of this group can cause severe disease when the hosts are under stressful conditions. The aim of this study was to identify living cultures from the Botryosphaeriaceae family preserved in the Queensland and Victorian Plant Pathology Herbaria using DNA sequence analyses. The 51 isolates were collected between 1971 and 2017, from 35 different host genera, with the dominant host genera being Mangifera (11 isolates), Acacia (10), and Persea (5). Multilocus sequence analyses resulted in the re-identification of 41 isolates to the genera Botryosphaeria (2 isolates), Diplodia (4), Dothiorella (1), Lasiodiplodia (19), and Neofusicoccum (15), as well as some that belonged to genera outside of the Botryosphaeriaceae (10). New records for Australia were Botryosphaeria sinensis, Diplodia alatafructa, Lasiodiplodia gonubiensis, Neofusicoccum cryptoaustrale,andN. mangroviorum. These were identified as a result of a workshop organised by the Subcommittee on Plant Health Diagnostics. The results of this study provide the fundamental information regarding the diversity of Botryosphaeriaceae species present in Australian. Keywords Biosecurity . Diagnostics . Taxonomy Introduction The Botryosphaeriaceae (Dothideomycetes: Botryosphaeriales) includes 24 genera of ecologically diverse fungi that occur as * Yu Pei Tan saprobes, endophytes or plant pathogens (Slippers et al.
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