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A B C D 1990年12月18日 第４種郵便物認可 ISSN 0914-5818 2014 VOL. 28 NO. 1 C 2014 T VOL. 28 NO. 1 IN （公開用） O ACTINOMYCETOLOGICA M Y C E T O L O G 日 本 I 放 C 線 菌 学 http://www. actino.jp/ 会 日本放線菌学会誌 第28巻 1 号 誌 Published by ACTINOMYCETOLOGICA VOL.28 NO.1, 2014 The Society for Actinomycetes Japan SAJ NEWS Vol. 28, No. 1, 2014 Contents Outline of SAJ: Activities and Membership S 2 List of new scientific names and nomenclatural changes in the phylum Actinobacteria validly published in 2013 S 4 Award Lecture S 25 55th Regular Colloquium S 39 Online access to The Journal of Antibiotics for SAJ members S 40 S1 Outline of SAJ: Activities and Membership The Society for Actinomycetes Japan (SAJ) searchers in foreign countries are welcome to join was established in 1955 and authorized as a scien- SAJ. For application of SAJ membership, please tific organization by Science Council of Japan in contact the SAJ secretariat (see below). Annual 1985. The Society for Applied Genetics of Actino- membership fees are currently 5,000 yen for active mycetes, which was established in 1972, merged in members, 3,000 yen for student members and SAJ in 1990. SAJ aims at promoting actinomycete 20,000 yen or more for supporting members (mainly researches as well as social and scientific exchanges companies), provided that the fees may be changed between members domestically and internationally. without advance announcement. The Activities of SAJ have included annual and regular scientific meetings, workshops and publica- The current members (April 2014 - March 2016) tions of The Journal of Antibiotics (the official jour- of the Board of Directors are: Hiroyuki Osada nal, joint publication with Japan Antibiotics Re- (Chairperson; RIKEN), Haruo Ikeda (Vice Chair- search Association), Actinomycetologica (Newslet- person; Kitasato Univ.), Tomohiko Tamura (Secre- ter) and laboratory manuals. Contributions to Inter- tary General; NITE), Takayuki Kajiura (Ajinomoto national Streptomyces Project (ISP) and Interna- Co., Inc.), Kenji Ueda (Nihon Univ.), Yojiro Anzai tional Symposium on Biology of Actinomycetes (Toho Univ.), Koji Ichinose (Musashino Univ.), Jun (ISBA) have also been SAJ's activities. In addition, Ishikawa (NIID), Takashi Sakai (Eisai Co., Ltd.), SAJ have occasional special projects such as the Kenji Arakawa (Hiroshima Univ.), Tohru Dairi publication of books related to actinomycetes: “At- (Hokkaido Univ.), Yasuhiro Onaka (Tokyo Univ.), las of Actinomycetes, 1997”, “Identification Manual Masahiro Sota (Nagase & Co., Ltd.), and Takuji of Actinomycetes, 2001” and “Digital Atlas of Ac- Kudo (RIKEN). tinomycetes, 2002” (http://www.actino/ DigitalAt- The members of the Advisory Board are: Yoko las/). These activities have been planned and orga- Takahashi, Kunimoto Hotta, Kozo Ochi, Yuzuru nized by the board of directors with association of Mikami, and Akira Yokota. executive committees consisting of active members who belong to academic and nonacademic organiza- Copyright: The copyright of the articles pub- tions. lished in Actinomycetologica is transferred from the authors to the publisher, The Society for Actinomy- The SAJ Memberships comprise active mem- cetes Japan, upon acceptance of the manuscript. bers, student members, supporting members and honorary members. Currently (as of Mar. 31, The SAJ Secretariat 2012), SAJ has about 413 active members including c/o Resource Collection Division (NBRC), student members, 22 oversea members, 11 honorary NITE Biological Resource Center, members, 5 oversea honorary members, 1 special National Institute of Technology and Evaluation member and 12 supporting members. The SAJ 2-5-8, Kazusakamatari, Kisarazu, members are allowed to join the scientific and social Chiba 292-0818, Japan meetings or projects (regular and specific) of SAJ on Phone: +81-438-20-5763 a membership basis and to browse The Journal of Antibiotics from a link on the SAJ website and will Fax: +81-438-52-2329 receive each issue of Actinomycetologica, currently E-mail: [email protected] published in June and December. Actinomycete re- S2 List of new scientific names and nomenclatural changes in the phylum Actinobacteria validly published in 2013 NEW CLASS Acidimicrobiia Norris 2013, class. nov. teria), part B, Springer New York, 2012, pp. Type order: Acidimicrobiales Stackebrandt et al. 2000-2001; Validation List no. 151 [Int. J. Syst. 1997. Evol. Microbiol., 2013, 63: 1577-1580, Reference: Bergey's Manual of Systematic Bac- doi:10.1099/ijs.0.052571-0]. teriology, second edition, vol. 5 (The Actinobac- teria), part B, Springer New York, 2012, p. 1968; Rubrobacteria Suzuki 2013, class. nov. Validation List no. 151 [Int. J. Syst. Evol. Mi- Type order: Rubrobacterales Rainey et al. 1997. crobiol., 2013, 63: 1577-1580, Reference: Bergey's Manual of Systematic Bac- doi:10.1099/ijs.0.052571-0]. teriology, second edition, vol. 5 (The Actinobac- teria), part B, Springer New York, 2012, pp. Coriobacteriia König 2013, class. nov. 2004-2005; Validation List no. 151 [Int. J. Syst. Type order: Coriobacteriales Stackebrandt et al. Evol. Microbiol., 2013, 63: 1577-1580, 1997. doi:10.1099/ijs.0.052571-0]. Reference: Bergey's Manual of Systematic Bac- teriology, second edition, vol. 5 (The Actino- Thermoleophilia Suzuki and Whitman 2013, bacteria), part B, Springer New York, 2012, p. class. nov. 1975; Validation List no. 151 [Int. J. Syst. Evol. Type order: Thermoleophilales Reddy and Gar- Microbiol., 2013, 63: 1577-1580, cia-Pichel 2009. doi:10.1099/ijs.0.052571-0]. Reference: Bergey's Manual of Systematic Bac- teriology, second edition, vol. 5 (The Actinobac- Nitriliruptoria Ludwig et al. 2013, class. nov. teria), part B, Springer New York, 2012, p. 2010; Type order: Nitriliruptorales Sorokin et al. 2009. Validation List no. 151 [Int. J. Syst. Evol. Mi- Reference: Bergey's Manual of Systematic Bac- crobiol., 2013, 63: 1577-1580, teriology, second edition, vol. 5 (The Actinobac- doi:10.1099/ijs.0.052571-0]. NEW ORDER Eggerthellales Gupta et al. 2013, ord. nov. 63: 3379-3397, doi:10.1099/ijs.0.048371-0. Type genus: Eggerthella Wade et al. 1999. A member of the class Coriobacteriia. Reference: Int. J. Syst. Evol. Microbiol., 2013, NEW FAMILY Atopobiaceae Gupta et al. 2013, fam. nov. Type 63: 3379-3397, doi:10.1099/ijs.0.048371-0. A genus: Atopobium Collins and Wallbanks, 1993. member of the order Coriobacteriales. Reference: Int. J. Syst. Evol. Microbiol., 2013, S3 Eggerthellaceae Gupta et al. 2013, fam. nov. Motilibacteraceae Lee 2013, fam. nov. Type genus: Eggerthella Wade et al. 1999. Type genus: Motilibacter Lee 2012 emend. Lee Reference: Int. J. Syst. Evol. Microbiol., 2013. 2013, 63: 3379-3397, Reference: Int. J. Syst. Evol. Microbiol., doi:10.1099/ijs.0.048371-0. 2013, 63: 3818-3822, A member of the order Eggerthellales. doi:10.1099/ijs.0.052357-0. A member of the suborder Frankineae. NEW GENUS Allonocardiopsis Du et al. 2013, gen. nov. N-acetyl Mur. Type species: Allonocardiopsis opalescens Du et Whole-cell sugar: Gal, Glc. al. 2013. Fatty acid: a-C17:0, a-C17:1ω9c. Reference: Int. J. Syst. Evol. Microbiol., 2013, Isoprenoid quinone: MK-8(H4). 63: 900-904, doi:10.1099/ijs.0.041491-0. Polar lipid: DPG, PG, PI, PGL, PL, GLs. A Gram-stain-positive, aerobic actinomycete. A member of the family Dermacoccaceae. Cell wall: meso-A2pm. Whole-cell sugar: Gal. Flaviflexus Du et al. 2013, gen. nov. Fatty acid: i-C16:0, a-C15:0, i-C15:0. Type species: Flaviflexus huanghaiensis Du et al. Isoprenoid quinone: MK-9, MK-11, MK-12. 2013. Polar lipid: PG, DPG. Reference: Int. J. Syst. Evol. Microbiol., 2013, A member of the suborder Streptosporangineae. 63: 1863-1867, doi:10.1099/ijs.0.042044-0. Gram-stain-positive, non-motile, straight to Aquihabitans Jin et al. 2013, gen. nov. slightly curved rods and facultatively anaerobic. Type species: Aquihabitans daechungensis Jin et Cell wall: type A5α al. 2013. (L-Lys-L-Ala-L-Lys-D-Glu). Reference: Int. J. Syst. Evol. Microbiol., 2013, Fatty acid: C18:1ω9c, C16:0, C14:0, C18:0, C16:1ω9c. 63: 2970-2974, doi:10.1099/ijs.0.046060-0. Isoprenoid quinone: MK-9(H4). Gram-stain-positive, non-motile short rods with Polar lipid: PG, PL, PGLs. cilia. A member of the family Actinomycetaceae. Fatty acid: C16:1ω5c, C16:0, C17:1ω8c, C18:1ω9c. Isoprenoid quinone: MK-9(H6). Jatrophihabitans Madhaiyan et al. 2013, gen. Polar lipid: DPG, PI, PIM, PL. nov. A member of the family Iamiaceae. Type species: Jatrophihabitans endophyticus Madhaiyan et al. 2013. Barrientosiimonas Lee et al. 2013, gen. nov. Reference: Int. J. Syst. Evol. Microbiol., 2013, Type species: Barrientosiimonas humi Lee et al. 63: 1241-1248, doi:10.1099/ijs.0.039685-0. 2013. Gram-stain-positive, aerobic, non-motile, Reference: Int. J. Syst. Evol. Microbiol., 2013, non-spore-forming rods. 63: 241-248, doi:10.1099/ijs.0.038232-0. Cell wall: type A1γ (meso-A2pm); N-glycolyl A Gram-stain-positive, aerobic, irregular cocci Mur. and short rods. Fatty acid: i-C16:0, C18:1ω9c, a-C17:0, C-17:1ω8c. Cell wall: type A4α (L-Lys-L-Ser-D-Asp); Isoprenoid quinone: MK-9(H4). S4 Polar lipid: DPG, PLs, GL, ALs. Polar lipid: DPG, PG, DMG, PL, GL. A member of the suborder Frankineae. A member of the family Microbacteriaceae. Longimycelium Xia et al. 2013, gen. nov. Parvibacter Clavel et al. 2013, gen. nov. Type species: Longimycelium tulufanense Xia et Type species: Parvibacter caecicola Clavel et al. al. 2013. 2013. Reference: Int. J. Syst. Evol. Microbiol., 2013, Reference: Int. J. Syst. Evol. Microbiol., 2013, 63: 2813-2818, doi:10.1099/ijs.0.044222-0. 63: 2642-2648, doi:10.1099/ijs.0.045344-0. An aerobic, Gram-stain-positive, non-acid-fast Aerotolerant, Gram-stain-positive rods that grow actinomycete forming abundant aerial mycelia only under strictly anoxic conditions. with spherical sporangia containing one to sev- Cell wall: meso-A2pm.
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    UC San Diego Research Theses and Dissertations Title Phylogenetic Diversity of Gram-positive Bacteria and Their Secondary Metabolite Genes Permalink https://escholarship.org/uc/item/06z0868t Author Gontang, Erin A Publication Date 2008 Peer reviewed eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA, SAN DIEGO Phylogenetic Diversity of Gram-positive Bacteria and Their Secondary Metabolite Genes A Dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Oceanography by Erin Ann Gontang Committee in charge: William Fenical, Chair Douglas H. Bartlett Bianca Brahamsha William Gerwick Paul R. Jensen Kit Pogliano 2008 3324374 3324374 2008 The Dissertation of Erin Ann Gontang is approved, and it is acceptable in quality and form for publication on microfilm: ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ ____________________________________ Chair University of California, San Diego 2008 iii DEDICATION To John R. Taylor, my incredible partner, my best friend and my love. ***** To my mom, Janet M. Gontang, and my dad, Austin J. Gontang. Your generous support and unconditional love has allowed me to create my future. Thank you. ***** To my sister, Allison C. Gontang, who is as proud of me as I am of her. You are a constant source of inspiration and I am so fortunate to have you in my life. iv TABLE OF CONTENTS
  • Gokul Jarishma Keriuscia Vol1

    Gokul Jarishma Keriuscia Vol1

    SUPRAGLACIAL SYSTEMS BIOLOGY OF DYNAMIC ARCTIC MICROBIAL ECOSYSTEMS A thesis submitted for the degree of Philosophiae Doctor (Doctor of Philosophy) by Jarishma Keriuscia Gokul (MSc, BTech (Hons), BSc) Interdisciplinary Centre for Environmental Microbiology Institute for Biological, Environmental and Rural Sciences Aberystwyth University May 2017 DECLARATION Word count of thesis: .……………………………………………………………………………….…………….57 348 DECLARATION This work has not previously been accepted in substance for any degree and is not being concurrently submitted in candidature for any degree. Candidate name ……………………………………………………………………………………………………….Jarishma Keriuscia Gokul Signature …………………………………………………………………………………………………………………. Date ………………………………………………………………………………………………………………………….30 May 2017 STATEMENT 1 This thesis is the result of my own investigations, except where otherwise stated. Where correction services have been used, the extent and nature of the correction is clearly marked in a footnote(s). Other sources are acknowledged by footnotes giving explicit references. A bibliography is appended. Signature …………………………………………………………………………………………………………………. Date ………………………………………………………………………………………………………………………….30 May 2017 STATEMENT 2 I hereby give consent for my thesis, if accepted, to be available for photocopying and for inter-library loan, and for the title and summary to be made available to outside organisations. Signature …………………………………………………………………………………………………………………. Date ………………………………………………………………………………………………………………………….30 May 2017 i SUMMARY Arctic glacier surfaces
  • Benemérita Universidad Autónoma De Puebla

    Benemérita Universidad Autónoma De Puebla

    BENEMÉRITA UNIVERSIDAD AUTÓNOMA DE PUEBLA INSTITUTO DE CIENCIAS CENTRO DE INVESTIGACIONES EN CIENCIAS MICROBIOLÓGICAS POSGRADO EN MICROBIOLOGÍA DIVERSIDAD BACTERIANA METILOTRÓFICA ASOCIADA A LA CACTÁCEA Neobuxbaumia macrocephala, ENDÉMICA EN RIESGO DE LA RESERVA TEHUACÁN CUICATLÁN TESIS QUE PARA OBTENER EL GRADO DE: DOCTORA EN CIENCIAS (MICROBIOLOGÍA) PRESENTA: MC MARÍA DEL ROCÍO BUSTILLOS CRISTALES ASESOR DE TESIS: DC LUIS ERNESTO FUENTES RAMÍREZ PUEBLA, PUE. AGOSTO, 2017 Índice RESUMEN………………………………………………………............................1 ABSTRACT………………………………………………………………………..2 INTRODUCCIÓN………………………………………………………………….3 ANTECEDENTES………………………………………………...........................10 JUSTIFICACIÓN………………………………………………………………….11 OBJETIVOS……………………………………………………………………….11 General…………………………………………………………………………...11 Particulares……………………………………………………………………… 11 MATERIALES Y MÉTODOS…………………………………………………….12 1. Zona de muestreo…………………………………………………………..12 2. Aislamiento………………………………………………………………...13 3. Caracterización Fenotípica………………………………………………....13 3.1 Morfología microscópica ……………………………………………... 13 3.2 Morfología colonial…………………………………………………… 13 3.3 Características bioquímicas y fisiológicas de los aislados……………. 13 3.3.1. Pruebas Bioquímicas……………………………………….. 14 3.3.2 Crecimiento en diferentes fuentes de carbono……………… 14 3.3.3. Determinación de condiciones óptimas de crecimiento……. 14 3.3.3.1 Crecimiento a diferentes temperaturas……………. 14 3.3.3.2. Crecimiento bacteriano a diferentes pH………….. 14 3.3.3.3. Tolerancia a la presencia de cloruro de sodio……..15 en el medio 3.3.4 Comportamiento antimicrobiano…………………………….15 3.3.5 Crecimiento en metanol dependiente de Ca2+ y Ce3+ 15 4. Caracterización genotípica 15 4.1 Extracción de DNA genómico 15 4.2 PCR para la amplificación del gen que codifica la subunidad 16S rRNA 16 4.3 Amplificación del gen que codifica la subunidad 23S rRNA 16 i 4.4 Determinación de genes que codifican para la enzima metanol deshidrogenasa 17 4.4.1 PCR para amplificar genes involucrados en la metilotrofía 17 4.4.2.