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DNA / RNA Extraction DNA / RNA Extraction – Key to Successful Analysis

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DNA / RNA Extraction DNA / RNA Extraction – Key to Successful Analysis BIOTECON Diagnostics DNA / RNA Extraction DNA / RNA Extraction – Key to Successful Analysis StarPrep Kits ShortPrep Kits Sample Preparation Kits Magnetic Preparation Kits Fast and easy lysis by mixing samples with buffer High purity DNA/RNA clean-up, manual column based or automated magnetic bead based Reliable analysis of food and feed samples with real-time extraction kits have been optimized in combination with PCR require appropriate sample preparation. Optimized our foodproof® real-time PCR Detection, Identification and and validated methods for the extraction of nucleic acids Quantification Kits. The isolated DNA/RNA can be used from any kind of biological material are of high importance. directly in all our real-time PCR assays. The processed sample must be free of contaminants, especially inhibitors, while retaining even small amounts Direct lysis, Column based or Automated of the target’s genetic information. The extraction method The foodproof® ShortPrep, StarPrep, Sample Preparation should be as fast and convenient as possible without and Magnetic Preparation Kits assist you in efficiently compromising safety or reproducibility. working with all types of sample matrices, including difficult complex samples like e.g. cheeses, spices or cacao. Optimized Combination of Extraction Kit and PCR Assay BIOTECON Diagnostics has developed a broad range of Succeed the challenges of complex matrices, PCR fast, safe and easy-to-use DNA and RNA extraction kits inhibitors and hidden targets with fast, straight-forward for any food, beverage or environmental sample. Our easy-to-use solutions. DNA / RNA Extraction – Key to Successful Analysis Table 1: Comparison of foodproof® extraction kits for food and beverage relevant parameters Rapid Highly purified Target Bead based/ Bulk Prefilled Column based Automated Sample Magnetic Gram-negative bacteria StarPrep One* ShortPrep I Preparation I Preparation I, V Sample Magnetic Gram-positive bacteria StarPrep Two* ShortPrep II Preparation II Preparation II Enterobacteriaceae, Magnetic StarPrep One - - Cronobacter Preparation IV Vibrio, STEC, StarPrep Three - - - Beer spoilage bacteria Dekkera, S. cerevisiae var. StarPrep Two StarPrep Four - - diastaticus Yeast & Mold StarPrep Two - - - Sample Magnetic GMOs, Animal ID, Allergens - - Preparation III Preparation III Sample Viruses - - - Preparation IV * High-throughput protocol available. See 8-strip DNA extraction protocols. StarPrep Kits: Versatile WORKFLOW e.g. StarPrep One Enjoy convenient and flexible DNA StarPrep can be used for rapid DNA extraction: the foodproof® StarPrep extraction from enrichment cultures, Kits are designed for quick and direct and rinse samples. The reagents ADD SAMPLE easy extraction of DNA from food contain no hazardous chemicals or CENTRIFUGE 5 min DISCARD SUPERNATANT and beverage samples by heat- compounds, unlike other sample induced lysis. StarPrep is a cost- preparation methods. The entire DNA effective, versatile solution for nearly preparation can be performed in a ADD LYSIS BUFFER all kind of foods, with optimized lysis single tube, minimizing handling steps, reagents and protocols for different reducing risk of cross-contamination applications. and exposure to bio-hazardous material. StarPrep One Kit Bulk solution for fast and flexible DNA extraction MIX Table 2: Overview of foodproof® StarPrep Kits foodproof® StarPrep One foodproof® StarPrep Two foodproof® StarPrep Three foodproof® StarPrep Four Gram-positive and other INCUBATE bacteria, e.g. Listeria, Vibrio, Dekkera, 10 min Gram-negative bacteria, Targets Campylobacter, E. coli STEC, S. cerevisiae var. e.g. Salmonella O157, Alicyclobacillus; Beer spoilage bacteria diastaticus Yeast and Mold Suitable for a wide range of food, animal feed and MIX Matrices Enrichment cultures, beverage samples environmental samples Mechanical disruption and Mechanical disruption and Principle Heat-induced lysis Heat-induced lysis heat-induced lysis heat-induced lysis CENTRIFUGE 1 min Isolations 100 / 500 96 96 96 Additional Resuspension Reagent, - - - Products Washing Solution SUPERNATANT MicroVal, AOAC, FOR DETECTION Approvals AOAC, NordVal and DAFF - - NordVal and DAFF ShortPrep Kits: Ready-to-Use WORKFLOW The fast and easy DNA extraction method for your enriched e.g. ShortPrep I samples: the foodproof® ShortPrep Kits are designed for quick and easy DNA extraction of bacterial DNA to be used directly for PCR. Simply add enriched sample to pre-aliquoted buffer, and in less than 30 minutes, samples from bacterial enrichment cultures are ready for PCR. The entire extraction ADD SAMPLE is performed in a single tube, minimizing handling steps and to prefilled lysis buffer reducing risk of cross-contamination. The lysis buffers are free of dangerous reagents. The kits are validated and certified by internationally MIX recognized institutions such as AOAC, NordVal and DAFF, and can be used for a variety of foods, like raw materials or processed food, environmental samples and pet foods. ShortPrep I Kit Prefilled tubes for fast DNA extraction INCUBATE in less than 30 minutes 10 min Table 3: Overview of foodproof® ShortPrep Kits foodproof® ShortPrep I foodproof® ShortPrep II MIX Gram-positive and other bacteria, e.g. Listeria, Targets Gram-negative bacteria, e.g. Salmonella Campylobacter and Alicyclobacillus Matrices* Suitable for a wide range of food, animal feed and environmental samples CENTRIFUGE Principle Heat-induced lysis Mechanical disruption and heat-induced lysis 1 min Convenience 96 individual ready-to-use reaction mixes for maximum efficiency and easy DNA extraction Approvals AOAC, NordVal and DAFF SUPERNATANT FOR DETECTION * DNA extracts from some raw materials of plant origin (e.g. spices) and from some beverage samples may still contain substances that interfere with PCR amplification or with fluorescent signal detection. For such materials, preparation and purification with the BIOTECON Diagnostics’ foodproof Sample Preparation Kit I and II are recommended. Sample Preparation Kits: High Quality Purification WORKFLOW e.g. Sample Preparation III Kit* Get rid of unwanted PCR contaminants and inhibitors: the foodproof® Sample Preparation Kits are designed to isolate and purify DNA or RNA even from challenging ADD EXTRACTION BUFFER matrices containing high amounts of PCR inhibitors. Kit to sample reagents and consumables are optimized to obtain the best results for each parameter. Protocols are easy to Sample Preparation III Kit MIX follow and implement in daily routine use. The quality of the INCUBATE 30 min Column-based DNA obtained with the kits is suitable for any application extraction for CENTRIFUGE 10 min using real-time PCR. highly purified DNA TRANSFER SUPERNATANT ADD BINDING BUFFER & PROTEINASE K Table 4: Overview of foodproof® Sample Preparation Kits INCUBATE 10 min ADD ISOPROPANOL foodproof® Sample foodproof® Sample foodproof® Sample foodproof® Sample Preparation I Kit Preparation II Kit Preparation III Kit Preparation IV Kit TRANSFER SUPERNATANT to filter column Gram-positive and other Genetically modified CENTRIFUGE 1 min Gram-negative bacteria, bacteria, e.g. Listeria, organisms (GMOs), Viruses Targets DISCARD FLOW THROUGH e.g. Salmonella Campylobacter and animal identification (e.g. Norovirus) REPEAT ONCE Alicyclobacillus allergens ADD WASH BUFFER Matrices Suitable for a wide range of food, beverages, animal feed and environmental samples CENTRIFUGE 1 min The RNA is bound to a DISCARD FLOW THROUGH The DNA is bound to a glass fiber filter, washed thoroughly and the pure DNA is eluted Principle glass fiber filter, washed REPEAT ONCE from the columns and eluted ADD ELUTION BUFFER Isolation of highly purified DNA, virtually free of other cellular components and PCR inhibitors CENTRIFUGE 1 min Isolation of viral RNA, Features Suitable for matrices containing high amount of PCR inhibitors FLOW THROUGH process control included FOR DETECTION Sample Preparation Kit II and Sample Preparation Kit III include a lysozym and Proteinase K digestion step, respectively, for maximum performance * Workflow for GMOs & allergens; Animal ID slightly different protocol Sample Preparation Kits: High Quality Purification Magnetic Preparation Kits: Automated Purification Automation Systems BIOTECON Diagnostics offers a variety of solutions The KingFisherTM Flex is a semi- for automated high-throughput molecular testing for automated system that allows fast and pathogens, GMOs, animal identification and allergens. convenient DNA extraction. The high The foodproof® Magnetic Preparation Kits are based on throughput system processes up to 96 magnetic bead technology, providing ultra-pure, ready- samples simultaneously; fewer samples to-use genomic DNA of bacterial, plant or animal origin may also be processed. for enhanced performance in sensitive real-time PCR Magnetic Preparation Kit I applications. The process proceeds with DNA being bound Bead-based DNA to magnetic beads and washed thoroughly through multiple extraction for steps. In the final step, highly purified DNA is eluted from automation the beads and is ready for use. Table 5: Overview of foodproof® Magnetic Preparation Kits KingFisherTM Flex ® ® ® ® ® foodproof MPK I foodproof MPK II foodproof MPK III foodproof MPK IV foodproof MPK V The Janus IntegratorTM allows for full automation of processing samples Gram-negative GMOs, Entero-bacteriaceae, Gram-positive Targets bacteria, e.g. animal identification, Cronobacter and Salmonella spp. through DNA extraction and PCR setup. bacteria, e.g. Listeria Salmonella spp.
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