CAMK2G (Human) Recombinant Protein

CAMK2G (Human) Recombinant Gene Alias: CAMK, CAMK-II, CAMKG, FLJ16043, Protein MGC26678

Gene Summary: The product of this gene belongs to Catalog Number: P5505 the Serine/Threonine protein kinase family, and to the Regulation Status: For research use only (RUO) Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of Product Description: Human CAMK2G (NP_751910.1, plasticity at glutamatergic synapses. In mammalian cells 1 a.a. - 518 a.a.) full-length recombinant protein with the enzyme is composed of four different chains: alpha, GST tag expressed in baculovirus infected Sf21 cells. beta, gamma, and delta. The product of this gene is a gamma chain. Six alternatively spliced variants that Host: Insect encode six different isoforms have been characterized to date. Additional alternative splice variants that encode Theoretical MW (kDa): 85 different isoforms have been described, but their full-length nature has not been determined. [provided by Applications: Func, SDS-PAGE RefSeq] (See our web site product page for detailed applications information)

Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols

Form: Liquid

Preparation Method: Baculovirus infected insect cell (Sf21) expression system

Purification: Glutathione sepharose chromatography

Purity: 63 % by SDS-PAGE/CBB staining

Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 uM.

Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Entrez GeneID: 818

Gene Symbol: CAMK2G

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