Product datasheet Anti-KCNQ5 Antibody Catalog Number: A04705-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-KCNQ5 Antibody Name KCNQ5 Source Rabbit IgG Species Reactivity human Tested Application WB,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human KCNQ5 recombinant (Position: E617-K932). Purification Immunogen affinity purified. Observed MW 102KD Dilution Ratios Western blot: 1:500-2000 Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Potassium voltage-gated channel subfamily KQT member 5 is a protein that in humans is encoded by the KCNQ5 gene. This gene is a member of the KCNQ gene family that is differentially expressed in subregions of the brain and in skeletal muscle. KCNQ5 is mapped to 6q13. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. KCNQ5 has been shown to interact with KvLQT3.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-KCNQ5 Antibody Catalog Number: A04705-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis analysis of anti- KCNQ5 using anti- KCNQ5 antibody (A04705-1).The antibody (A04705-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing mouse brain tissue. conditions.Lane 1: human Biotinylated goat anti- PC-3 whole cell rabbit IgG was used as lysates,Lane 2: human secondary antibody. The HEK293 whole cell tissue section was lysates. Use rabbit anti- developed using Strepavi KCNQ5 1:1000, probed din-Biotin-Complex with a goat anti-rabbit (SABC) (Catalog # IgG-HRP secondary SA1022) with DAB as the antibody. The signal is chromogen. developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for KCNQ5 at approximately 102KD. The expected band size for KCNQ5 is at 102KD.

Figure 3. IHC analysis Figure 4. IHC analysis using anti- KCNQ5 using anti- KCNQ5 antibody (A04705-1). antibody (A04705-1). detected in paraffin- detected in paraffin- embedded section of rat embedded section of brain tissue. Biotinylated human renal cancer goat anti-rabbit IgG was tissue. Biotinylated goat used as secondary anti-rabbit IgG was used antibody. The tissue as secondary antibody. section was developed The tissue section was using Strepavidin-Biotin- developed using Strepavi Complex (SABC) (Catalog din-Biotin-Complex # SA1022) with DAB as (SABC) (Catalog # the chromogen. SA1022) with DAB as the chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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