Anti-TANK1 / Tankyrase 1 Antibody (ARG64773)

ARG64773 Package: 100 μg anti-TANK1 / Tankyrase 1 antibody Store at: -20°C

Summary

Product Description Goat Polyclonal antibody recognizes TANK1 / Tankyrase 1

Tested Reactivity Hu

Tested Application WB

Specificity Fusion protein containing 120 amino acids from N-Terminal region of the protein sequence according to NP_003738.2. No cross reactivity expected to TANK2.

Host Goat

Clonality Polyclonal

Isotype IgG

Target Name TANK1 / Tankyrase 1

Antigen Species Human

Immunogen Recombinant fusion protein containing 120 a.a. from N-terminal region to TANK1 (according to NP_079511.1).

Conjugation Un-conjugated

Alternate Names EC 2.4.2.30; Tankyrase I; ARTD5; TRF1-interacting ankyrin-related ADP-ribose polymerase; TIN1; TINF1; PARPL; Poly [ADP-ribose] polymerase 5A; PARP5A; TANK1; pART5; TNKS1; TNKS-1; Tankyrase-1; PARP-5a; ADP-ribosyltransferase diphtheria toxin-like 5

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 142 kDa

Properties

Form Liquid

Purification Purified from goat serum by ammonium sulphate precipitation followed by affinity chromatography using Protein G.

Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA

Preservative 0.02% Sodium azide

Stabilizer 0.5% BSA

Concentration 0.5 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links GeneID: 8658 Human

Swiss-port # O95271 Human

Gene Symbol TNKS

Gene Full Name tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase

Function Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP- ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity. [UniProt]

Research Area Gene Regulation antibody

Images

ARG64773 anti-Tankyrase / TANK1 antibody WB image

Western Blot: 1). Mock transfection; 2) TANK1 (lane 2) and TANK2 (lane 4) expressing plasmid transfected HEK293 cell lysate standed with ARG64773 anti-Tankyrase / TANK1 antibody. Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4°C.