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Each Trait That Mendel Studied Existed in 2 Discrete Forms

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Each Trait That Mendel Studied Existed in 2 Discrete Forms Patterns of Inheritance Objective # 1 In this section we will examine how studying patterns of inheritance has Define the term “monohybrid cross”. allowed scientists to learn about some Describe one of the monohybrid of the basic laws that govern the crosses carried out by Mendel and transmission of genetic information explain how the results of these from one generation to the next. crosses led him to formulate the Law of Segregation and the Law of You will also learn how to use this Dominance. knowledge to predict the outcome of certain types of genetics crosses. 1 2 Objective 1 Objective 1 So far, we have focused on how However, over 50 years before scientists chromosomes get passed from cell to cell understood the role that chromosomes during the eukaryotic cell cycle, and from play in transmitting hereditary one generation to the next during information, an Austrian monk named eukaryotic life cycles. Gregor Mendel discovered the basic Since chromosomes contain the principles of heredity by studying the hereditary information, this shows us how pattern of inheritance for 7 different the hereditary information is transmitted traits in pea plants. from one generation to the next. 3 4 Objective 1 Objective 1 Each trait that Mendel studied existed in 2 The 7 traits that discrete forms. Mendel studied are For example, seed shown in the chart shape could be on the right. wrinkled or Each trait exists in round: 2 forms. 5 6 1 Objective 1 Objective 1 Mendel was successful where many before him had failed because: ¾ he used statistics to mathematically ¾ he simplified the problem by first analyze the results of his crosses and examining just one trait at a time. help determine the general patterns of his results. ¾ he limited his study to traits that existed in 2 discrete forms. ¾ he kept quantitative data over several generations, using large sample sizes. 7 8 Objective 1 Objective 1 Mendel’s success was also due to a Mendel began with crosses where he certain amount of luck because each followed the inheritance of one trait. trait that he followed happened to be Although he didn’t know it, each trait controlled by a single pair of alleles. he studied was controlled by a single This produces the simplest possible gene locus. This type of cross is called a pattern of inheritance. monohybrid cross. For example, Mendel followed the inheritance of flower color, which exists in 2 discrete forms: purple and white. 9 10 Objective 1 Objective 1 When he crossed some pure-breeding Next, he allowed the F1 plants to self- purple flowered plants with some fertilize in order to produce the F2 pure-breeding white flowered plants generation. Again, the results were (this is called the parental or P unexpected: generation) he got some surprising results: ¾ Out of 929 F2 plants, he found 705 ¾ all of the offspring (called the F1 with purple flowers and 224 with white generation) had purple flowers! flowers, a ratio of 3.15 to 1. 11 12 2 Objective 1, One of Mendel’s Monohybrid Crosses Objective 1 Mendel explained his results by proposing the following 4-part hypothesis: 1)Each individual has two “hereditary factors” controlling a given trait. The pure-breeding purple parents have 2 hereditary factors for purple flowers, and the pure-breeding white plants have 2 hereditary factors for white flowers. 13 14 Objective 1 Objective 1 Today we call Mendel’s “hereditary The appearance of an organism is factors” alleles. Scientists use letters to called its phenotype (e.g. purple represent alleles. For example, if we use flowers), and the alleles the organism “A” to represent the purple allele and “a” has is its genotype (e.g. AA). to represent the white allele, then the pure-breeding purple plants would be AA and the pure-breeding white plants would be aa. 15 16 Objective 1 Objective 1 From chromosome studies we know that parents are diploid, so there are 2 If the 2 alleles are identical (e.g. AA), sets of chromosomes in each cell, and the individual is homozygous for that 2 alleles at each gene locus. trait. If the 2 alleles are different (e.g. Aa), the individual is heterozygous for Because flower color is controlled by that trait. one gene locus, each parent must have 2 alleles controlling this trait. 17 18 3 Objective 1 Objective 1 2) When the parents produce gametes, Today we know that homologous the 2 hereditary factors separate, and chromosomes separate during meiosis I, each gamete receives one of the 2 leading to formation of haploid gametes. factors. Therefore, all gametes Because gametes are haploid, each produced by the purple parent (AA) gamete has 1 set of chromosomes, and 1 have one purple allele (A), and all allele at every gene locus. gametes produced by the white parent Because flower color is controlled by one (aa) have 1 white allele (a). This is gene locus, each gamete must have 1 called Mendel’s Law of Segregation. 19 allele controlling this trait. 20 Objective 1 Objective 1 3) The offspring are formed when a Today we know the offspring are gamete from one parent joins with a diploid, so there are 2 sets of gamete from the other parent. chromosomes in each cell, and 2 alleles Therefore, each F1 offspring receives at each gene locus (one inherited from one purple hereditary factor (A) each parent). from the purple parent (AA) and one white hereditary factor (a) from the white parent (aa). 21 22 Objective 1 Objective 1 4) When an individual is heterozygous, One the next slide, you can see how only one of the 2 alleles is expressed. Mendel’s Laws of Inheritance can be Mendel called the expressed allele used to correctly predict the outcome dominant, and the non-expressed of both the F1 and the F2 generations: allele recessive. Because purple is dominant to white, all the F1 plants (Aa) have purple flowers. This is known as Mendel’s Law of dominance. 23 24 4 Objective 1, One of Mendel’s Monohybrid crosses Objective # 2 Explain how a testcross can be used to determine whether an individual with a dominant phenotype is homozygous or heterozygous. 25 26 Objective 2 Objective 2 To determine whether an individual with a dominant phenotype is homozygous for the dominant allele or heterozygous, Mendel crossed the individual in question with an individual that had the recessive phenotype: 27 28 Objective # 3 Objective 3 In a dihybrid cross, we follow alleles at 2 Define the term “dihybrid cross”. gene loci simultaneously: Describe one of the dihybrid ¾ the parents are diploid, so there are 2 crosses carried out by Mendel and alleles at each gene locus = 4 alleles total explain how the results of these ¾ the gametes are haploid, so there is 1 crosses led him to formulate the allele at each gene locus = 2 alleles total Law of Independent Assortment. ¾ the offspring are diploid, so there are 2 alleles at each gene locus = 4 alleles total 29 30 5 Objective 3 Objective 3 For example, in pea plants seed shape is With his monohybrid crosses, Mendel controlled by one gene locus where determined that the 2 alleles at a single round (R) is dominant to wrinkled (r) gene locus segregate when the gametes while seed color is controlled by a are formed. different gene locus where yellow (Y) is With his dihybrid crosses, Mendel was dominant to green (y). interested in determining whether alleles Mendel crossed 2 pure-breeding plants: at 2 different gene loci segregate one with round yellow seeds and the dependently or independently. other with green wrinkled seeds. 31 32 Objective 3 Objective 3, Dependent Assortment R R r r Dependent segregation (assortment) Parents Y Y y y means alleles at the 2 gene loci R r segregate together, and are transmitted Parental Gametes Y y as a unit. Therefore, each plant would F Offspring R r only produce gametes with the same 1 Y y combinations of alleles present in the F Offspring’s Gametes R r gametes inherited from its parents: 1 Y y What is the expected 33 phenotypic ratio for the F2? 34 Objective 3 Objective 3 F2 with dependent assortment: Independent segregation (assortment) R r means alleles at the 2 gene loci Y y segregate independently, and are NOT R R R R r transmitted as a unit. Therefore, each Y Y Y Y y plant would produce some gametes R r r r with allele combinations that were not r present in the gametes inherited from Y y y y y its parents: Ratio is 3 round, yellow : 1 wrinkled, green 35 36 6 Objective 3, Independent Assortment Objective 3 R R r r F2 with independent assortment: Parents Y Y y y RY Ry rY ry R r Parental Gametes RR RR Rr Rr Y y RY R r YY Yy YY Yy F1 Offspring RR RR Rr Rr Y y Ry Yy Yy Yy yy F1 Offspring’s R R r r Rr Rr rr rr Gametes rY Y y Y y YY Yy YY Yy Rr Rr rr rr What is the expected ry Yy yy Yy yy phenotypic ratio for the F2? 37 Phenotypic ratio is 9 : 3 : 3 : 1 38 Objective 3 Objective # 4 Mendel’s dihybrid crosses showed a Explain why some genes do 9:3:3:1 phenotypic ratio for the F2 generation. NOT assort independently. Based on these data, he proposed the Also explain how an experiment Law of Independent Assortment, by Morgan originally which states that when gametes form, demonstrated this.
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