FOXJ2 (NM 018416) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC218199

FOXJ2 (NM_018416) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: FOXJ2 (NM_018416) Human Tagged ORF Clone Tag: Myc-DDK Symbol: FOXJ2 Synonyms: FHX Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 4 FOXJ2 (NM_018416) Human Tagged ORF Clone – RC218199

ORF Nucleotide >RC218199 representing NM_018416 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGCTTCTGACCTAGAGAGTAGCCTCACCTCCATAGACTGGCTCCCCCAGCTGACCCTCCGAGCTACCA TTGAGAAGCTTGGAAGTGCCTCCCAGGCTGGGCCTCCCGGGAGCAGCCGCAAGTGTTCACCAGGGTCACC CACAGATCCTAATGCCACCCTGAGCAAAGACGAGGCAGCAGTGCACCAGGACGGCAAGCCACGATACAGC TATGCCACTCTCATCACCTATGCCATCAACTCCTCTCCAGCCAAGAAGATGACCCTCAGCGAGATTTACC GCTGGATCTGTGATAACTTCCCCTATTACAAGAATGCTGGCATTGGTTGGAAGAATTCAATACGGCACAA CCTTTCTCTCAACAAGTGTTTCCGGAAGGTGCCCAGACCTCGGGATGACCCTGGGAAGGGTTCCTATTGG ACAATTGACACCTGCCCTGACATTTCCCGAAAGAGAAGACACCCTCCAGATGATGATCTGTCCCAAGACT CACCAGAACAGGAGGCAAGCAAGAGCCCACGGGGAGGCGTTGCAGGGAGTGGAGAAGCCTCACTGCCTCC TGAGGGGAATCCGCAGATGTCACTTCAGAGCCCCACATCTATAGCCAGCTACAGCCAGGGCACAGGATCT GTGGATGGTGGAGCAGTGGCAGCAGGGGCTTCAGGCCGAGAAAGTGCTGAGGGTCCCCCTCCCCTCTATA ACACCAACCATGACTTTAAATTCTCCTACTCAGAGATCAACTTTCAGGATCTAAGCTGGTCCTTCCGCAA CCTCTATAAGTCCATGCTGGAGAAGTCCTCTTCCTCCTCTCAGCACGGCTTTTCTTCTCTCCTGGGGGAC ATCCCACCCTCGAACAACTACTACATGTATCAGCAGCAGCAGCCACCGCCACCTCAACAGCAGCAGCAGC AGCAGCAGCCGCCACAGCCACCTCCCCAGCAGTCCCAGCCACAGCAGCAGCAGGCACCTGCCCAGGGCCC CTCAGCTGTAGGGGGTGCTCCTCCACTGCACACCCCAAGCACAGATGGTTGTACCCCACCAGGGGGAAAG CAAGCTGGGGCGGAAGGCTATGGGCCTCCCCCTGTAATGGCCATGCATCCACCCCCGCTGCAGCATGGAG GCTACCACCCTCATCAGCACCATCCCCACTCCCACCCTGCCCAGCAGCCACCACCTCCACAGCCACAGGC ACAAGGCCAGGCTCCCATCAACAACACTGGCTTTGCCTTTCCTTCTGACTGGTGCTCTAATATTGACTCT TTAAAGGAAAGCTTCAAGATGGTGAATCGGCTCAATTGGTCCAGCATTGAGCAGTCACAATTCTCAGAAC TGATGGAGAGTCTACGACAGGCAGAGCAGAAGAACTGGACCCTCGACCAGCATCACATTGCCAATCTGTG TGACTCCCTCAACCACTTCCTTACTCAGACTGGTCACGTGCCCCCTCAAGGGGGTACCCACCGCCCACCA GCCCCTGCCCGTATTGCTGACTCCTGTGCCCTCACCAGTGGCAAACAGGAGTCAGCCATGAGCCAAGTGA ACTCTTATGGGCACCCACAAGCTCCCCACCTCTACCCTGGCCCATCACCAATGTACCCAATCCCCACCCA GGACTCAGCAGGATACAATCGCCCAGCACACCATATGGTCCCTCGGCCATCAGTGCCACCTCCTGGTGCC AATGAGGAGATCCCTGATGACTTCGACTGGGACTTGATCACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

Protein Sequence: >RC218199 representing NM_018416 Red=Cloning site Green=Tags(s)

MASDLESSLTSIDWLPQLTLRATIEKLGSASQAGPPGSSRKCSPGSPTDPNATLSKDEAAVHQDGKPRYS YATLITYAINSSPAKKMTLSEIYRWICDNFPYYKNAGIGWKNSIRHNLSLNKCFRKVPRPRDDPGKGSYW TIDTCPDISRKRRHPPDDDLSQDSPEQEASKSPRGGVAGSGEASLPPEGNPQMSLQSPTSIASYSQGTGS VDGGAVAAGASGRESAEGPPPLYNTNHDFKFSYSEINFQDLSWSFRNLYKSMLEKSSSSSQHGFSSLLGD IPPSNNYYMYQQQQPPPPQQQQQQQQPPQPPPQQSQPQQQQAPAQGPSAVGGAPPLHTPSTDGCTPPGGK QAGAEGYGPPPVMAMHPPPLQHGGYHPHQHHPHSHPAQQPPPPQPQAQGQAPINNTGFAFPSDWCSNIDS LKESFKMVNRLNWSSIEQSQFSELMESLRQAEQKNWTLDQHHIANLCDSLNHFLTQTGHVPPQGGTHRPP APARIADSCALTSGKQESAMSQVNSYGHPQAPHLYPGPSPMYPIPTQDSAGYNRPAHHMVPRPSVPPPGA NEEIPDDFDWDLIT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mk6045_a12.zip Restriction Sites: SgfI-MluI

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 FOXJ2 (NM_018416) Human Tagged ORF Clone – RC218199

Cloning Scheme:

Plasmid Map:

ACCN: NM_018416 ORF Size: 1722 bp

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 FOXJ2 (NM_018416) Human Tagged ORF Clone – RC218199

OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_018416.3 RefSeq Size: 5499 bp RefSeq ORF: 1725 bp Locus ID: 55810 UniProt ID: Q9P0K8 Domains: FH Protein Families: Transcription Factors MW: 62.2 kDa Gene Summary: Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.[UniProtKB/Swiss-Prot Function]

Product images:

Western blot validation of overexpression lysate (Cat# [LY402681]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218199 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).