MBD3 Antibody Cat. No.: 25-590

MBD3 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human MBD3.

TESTED APPLICATIONS: ELISA, WB

MBD3 antibody can be used for detection of MBD3 by ELISA at 1:312500. MBD3 antibody APPLICATIONS: can be used for detection of MBD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Tranfected 293T Cell Lysate

PREDICTED MOLECULAR 33 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

September 29, 2021 1 https://www.prosci-inc.com/mbd3-antibody-25-590.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store MBD3 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: MBD3

ALTERNATE NAMES: MBD3,

ACCESSION NO.: NP_003917

PROTEIN GI NO.: 4505119

GENE ID: 53615

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl- CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human BACKGROUND: proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this . Please see the Gene record to access additional publications.

REFERENCES: 1) Brown, S.E. (2007) Mol. Cell. Biol. 27 (13), 4938-4952.

ANTIBODIES FOR RESEARCH USE ONLY.

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