Catalog # Aliquot Size

P48-911C-05 3 x 5 nmol P48-911C-20 3 x 20 nmol P48-911C-50 3 x 50 nmol

PTPRA siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P48-911C

Lot # Z2085-27

Specificity Formulation PTPRA siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPRA expression. shipped at room temperature.

Product Description Reconstitution Protocol PTPRA siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPRA mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002836. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot LRP, HLPR, PTPA, HEPTP, HPTPA, RPTPA, PTPRL2, HPTP, R- 1x stock solutions for storage. PTP- Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the PTPRA (LRP), Active P48-21G lyophilized and resuspended siRNAs are stored at or PTPRC (CD45), Active P50-21H below -20oC. After resuspension, siRNA stock solutions ≥2 PTPRC (CD45), Active P50-21BG µM can undergo up to 50 freeze-thaw cycles without PTPRE, Active P52-21G significant degradation. For long-term storage, it is PTPRF (LAR), Active P53-21G recommended that the siRNA is stored at -70oC. For most PTPRS(PTP-sigma), P64-21G favorable performance, avoid repeated handling and Active multiple freeze/thaw cycles. PTPRM (PTP-mu) P58-35G

Scientific Background PTPRA (also known as LRP) is a member of the protein tyrosine phosphatase family and contains an extracellular PTPRA siRNA Set I siRNA duplexes targeted against three exon regions domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. PTPRA Catalog # P48-911C dephosphorylates SRC family kinases, potassium channels Lot # Z2085-27 and NMDA receptors and exists as three alternatively Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol spliced variants which encode two distinct isoforms (1). Format Lyophilized powder Absence of PTPRA compromises correct positioning of Stability 1yr at -70oC from date of shipment pyramidal neurons during development of mouse Storage & Shipping The lyophilized powder is stable for at hippocampus (2). least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Matthews, R J. et al: Identification of an additional siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles member of the protein-tyrosine-phosphatase family: without significant degradation. For evidence for alternative splicing in the tyrosine long-term storage, it is recommended phosphatase domain. Proc. Nat. Acad. Sci. 87: 4444- that the siRNA is stored at -70oC. For most favorable performance, avoid 4448, 1990. repeated handling and multiple 2. Petrone, A. et al: Receptor protein tyrosine freeze/thaw cycles. phosphatase alpha is essential for hippocampal neuronal migration and long-term potentiation. EMBO J. 2003 Aug 15;22(16):4121-31.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.