Helminthosporium Erythrinicola Fungal Planet Description Sheets 357

356 Persoonia – Volume 42, 2019

Helminthosporium erythrinicola Fungal Planet description sheets 357

Fungal Planet 894 – 19 July 2019 Helminthosporium erythrinicola Crous & M.J. Wingf., sp. nov. Etymology. Name refers to Erythrina, the host genus from which this Notes — Helminthosporium was recently revised by Vogl- fungus was isolated. mayr & Jaklitsch (2017) and Hernández-Restrepo et al. (2018). Classification — Massarinaceae, Pleosporales, Dothideo Helminthosporium erythrinicola is related to H. genistae (CBS mycetes. 142597), and represents the first species described from Erythrina humeana. Helminthosporium erythrinae (on Erythrina Colony on natural substrate black, hairy, effuse, 1–2 cm long. suberosa, India; conidia 4–8-septate, 39–62 × 8 mm; Thirumal- Mycelium mostly immersed, forming a brown stroma on the achar 1950) differs in having smaller conidia with fewer septa. surface, 150–200 mm diam, giving rise to erect, flexuous conidi- Based on a megablast search of NCBIs GenBank nucleotide ophores. Conidiophores 500–1200 × 6–10 mm, multiseptate, database, the closest hits using the ITS sequence had high- finely roughened, subcylindrical with slight apical taper, arising est similarity to Helminthosporium submersum (as Helmin in fascicles, unbranched, brown, becoming pale brown at apex, thosporium sp. ZLL-2017a, GenBank MG098780.1; Identi- rejuvenating percurrently. Conidiogenous cells terminal and ties = 462/483 (96 %), 4 gaps (0 %)), Helminthosporium intercalary with well-defined pores (4–5 × 2–3 mm), thickened velutinum (GenBank JN198435.1; Identities = 473/499 (95 %), and darkened, 25–40 × 6–8 mm. Conidia (70–)80–90(–110) × 4 gaps (0 %)) and Helminthosporium magnisporum (GenBank (9–)10–11(–12) mm, obclavate, straight to curved, apex subob- AB811452.1; Identities = 436/461 (95 %), 3 gaps (0 %)). Closest tuse, smooth, medium brown, (6–)7–8(–12)-distoseptate, with hits using the LSU sequence are Helminthosporium veluti angular lumina; wall 3–4 mm thick, hila thickened, darkened, num (GenBank KY984355.1; Identities = 814/823 (99 %), 3–4 mm diam. 1 gap (0 %)), Helminthosporium oligosporum (GenBank Culture characteristics — Colonies flat, spreading, with KY984333.1; Identities = 813/823 (99 %), 1 gap (0 %)) and Hel moderate aerial mycelium and feathery margin on PDA, smooth minthosporium caespitosum (GenBank KY984305.1; Identities on OA and MEA, reaching 60 mm diam after 2 wk at 25 °C. On = 813/823 (99 %), 1 gap (0 %)). Closest hits using the rpb2 MEA surface olivaceous grey, reverse iron-grey with patches sequence had highest similarity to Helminthosporium geni of olivaceous grey. On PDA surface and reverse iron-grey. On stae (GenBank KY984377.1; Identities = 832/884 (94 %), no OA surface iron-grey. gaps), Helminthosporium quercinum (GenBank KY984401.1; Typus. South Africa, Eastern Cape Province, Haga Haga, Amathole, on Identities = 828/884 (94 %), no gaps) and Helminthosporium leaves of Erythrina humeana (Fabaceae), 26 Dec. 2016, M.J. Wingfield, HPC velutinum (GenBank KY984416.1; Identities = 826/884 (93 %), 2301 (holotype CBS H-23961, culture ex-type CPC 35291 = CBS 145569, no gaps). ITS, LSU and rpb2 sequences GenBank MK876391.1, MK876432.1 and MK876486.1, MycoBank MB830852).

Colour illustrations. Erythrina humeana at Haga Haga. Sporulation on host tissue; conidiogenous loci and conidia. Scale bars = 10 µm.

Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected] Michael J. Wingﬁeld, Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Hatﬁeld 0028, Pretoria, South Africa; e-mail: [email protected]