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Blood Group Antigen Lewis B Mouse Monoclonal Antibody [Clone SPM194]

Catalog No Format Size Price (USD)

2525-MSM1X-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00

2525-MSM1X-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00

2525-MSM1X-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 459.00

Human Entrez Gene ID 2525 Immunogen Mucin isolated from a human ovarian cyst fluid

Human SwissProt P21217 Host / Ig Isotype Mouse / IgG1, kappa

Human Unigene 169238 Mol. Weight of Antigen 45kDa

Human Gene Symbol FUT3 Cellular Localization Cytoplasmic

Human Chromosome 19p13.3 Species Reactivity Human. Guinea Pig. Location Positive Control Colon. Synonyms group Lewis alpha-4-fucosyltransferase; ; Fucosyltransferase III; FucT-III; FUT3; Galactoside 3(4)-L-fucosyltransferase; gastric mucin; leB; lewis antigen system; lewis B blood group antigen; Lewis FT; major airway glycoprotein

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194). Specificity & Comments Supplied As The Lewis histo-blood group system comprises a set of fucosylated 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. glycosphingolipids that are synthesized by exocrine epithelial cells and Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available circulate in body fluids. The glycosphingolipids function in embryogenesis, WITHOUT BSA & azide at 1.0mg/ml. tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to Storage and Stability their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 in the last step of Lewis antigen biosynthesis. It encodes an enzyme with to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. required. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have Limitations been described in gastric carcinoma and associated lesions. Anomalous This antibody is available for research use only and is not approved for use expression of Lewis B antigen has been found in some non-secretory in diagnosis. gastric carcinomas and colorectal cancers.

Known Applications & Suggested Dilutions Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at Warranty RT)(Staining of formalin-fixed tissues requires heating tissue sections in There are no warranties, expressed or implied, which extend beyond this 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by description. Company is not liable for any personal injury or economic cooling at RT for 20 minutes) loss resulting from this product. Optimal dilution for a specific application should be determined.

Key References

1. Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara. Alteration of sialyl Lewis epitope expression in pterygium. J.Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631. 2. Bara, J. et al. 1988. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem. J. 254: 185-193. 3. Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J. Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30. 4. Richman, P.I. et al. 1987. Monoclonal antibodies to human colorectal : markers for differentiation and tumour characterization. Int. J. Cancer. 39: 317-328. 5. Rouger, P.h., et al, eds. 1987. Proceedings of the first international work- shop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987). Immunohaematology 30: 353- 720.

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