For Research Use Only

PRND Polyclonal antibody Catalog Number:26947-1-AP www.ptglab.com

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 26947-1-AP BC043644 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 350 μg/ml by 23627 WB 1:1000-1:6000 Nanodrop; Full Name: IHC 1:50-1:500 Source: prion 2 (dublet) IF 1:50-1:500 Rabbit Calculated MW: Isotype: 176 aa, 20 kDa IgG Observed MW: Immunogen Catalog Number: 17-20, 30 kDa AG25523

Applications Tested Applications: Positive Controls: IF, IHC, WB, ELISA WB : Glucosidase treated mouse testis tissue, mouse Species Specificity: testis tissue, rat testis tissue human, mouse, Rat IHC : mouse testis tissue, Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen IF : mouse testis tissue, retrieval may be performed with citrate buffer pH 6.0

The prion family is mainly composed of three ; PRNP, encoding the prion protein (PrP), PRND, encoding Background Information Doppel (Dpl), both located on the same genomic locus on mouse 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on , approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

Various lysates were subjected to SDS PAGE Non-treated mouse testis and glucosidase treated Immunohistochemical analysis of paraffin- followed by western blot with 26947-1-AP (PRND mouse testis tissue were subjected to SDS PAGE embedded mouse testis tissue slide using 26947-1- antibody) at dilution of 1:5000 incubated at room followed by western blot with 26947-1-AP (PRND AP (PRND antibody) at dilution of 1:200 (under 10x temperature for 1.5 hours. antibody) at dilution of 1:3000 incubated at room lens). Heat mediated antigen retrieval with Tris- temperature for 1.5 hours. EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1- AP) at dilution of 1:200 and CoraLite®488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).