bioRxiv preprint doi: https://doi.org/10.1101/2021.02.18.431837; this version posted February 18, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Induction of specific antibodies, IgG-secreting plasmablasts and memory B cells following BCG vaccination Julia Bitencourt1,2, Morven Wilkie1, Marco Polo Peralta Alvarez1, Ashley Jacobs1,3, Daniel Wright1, Stephanie A. Harris1, Steven G. Smith4,5, Sean Elias1, Andrew White6, Sally Sharpe6, Matthew K. O’Shea1,7, Helen McShane1, Rachel Tanner1*. 1The Jenner Institute, University of Oxford, UK; 2Gonҫalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil; 3University of Cape Town, Cape Town, South Africa; 4London School of Hygiene and Tropical Medicine, UK; 5Division of Biosciences, Brunel University, UK; 6Public Health England, Porton Down, Salisbury, UK; 7Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK. *Corresponding author:
[email protected] The Jenner Institute, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford, OX3 7DQ. +44(0)1865617615. Abstract Many tuberculosis (TB) vaccine candidates are designed as a boost to BCG; an understanding of the BCG-induced immune response is therefore critical, and the opportunity to relate this to circumstances where BCG does protect may direct the design of more efficacious vaccines. While the T cell response to BCG vaccination has been well-characterised, little is known about the B cell and antibody response.