Roczniki Akademii Rolniczej w Poznaniu CCCLXXXVIII
Botanika – Steciana , , - www.up.poznan.pl/steciana ISSN -
MORPHOLOGICAL DIFFERENTIATION OF POLLEN OF LYSIMACHIA VULGARIS L. FROM POLAND
DOROTA WROŃSKA-PILAREK, MARIA MOROZOWSKA
D. Wrońska-Pilarek, Department of Forestry Natural Foundations, Poznań University of Life Sciences, Wojska Polskiego D, - Poznań, Poland, e-mail: [email protected] M. Morozowska, Department of Botany, Poznań University of Life Sciences, Wojska Polskiego C, - Poznań, Poland, e-mail: [email protected]
(Received: March , . Accepted: June , )
ABSTRACT. The morphology and variability of pollen of Lysimachia vulgaris L. were studied on the base of the plant material coming from native localities. pollen grains were examined. The diagnostic features of pollen grains of studied species are: exine sculpture irregularly reticulate with lumina of dif- ferent diameters, presence of many minute granules of diff erent size inside lumina (a new feature for the examined species), muri with medium thickness, pollen shape most often subprolate, rarely prolate. Ob- tained results do not fully correspond with the available L. vulgaris pollen descriptions. Statistical analysis of nine quantitative grain characteristics showed their variability to be very diff erentiated. The highest variability was found to occur in trait the Exe/E (the distance between the apices of two ectocolpi). No de- pendence in pollen morphology diff erentiation due to the localization of natural populations was observed.
KEY WORDS: Lysimachia vulgaris, pollen morphology, statistical analyse, LM, SEM
INTRODUCTION of Lysimachia done by BENNELL and HU ( ) resulted in the recognition of ten major pollen-types and four Genus Lysimachia L. consists of ca. species of subtypes. Lysimachia vulgaris pollen was classifi ed by perennial, biennial or annual herbs with almost all over these authors into Fraseri-pollen type, together with the world distribution (STÅHL and ANDERBERG ). several other species e.g. L. davurica, L. fraseri, L. ter- Lysimachia is traditionally placed in the tribe Lysima- restris, L. thyrsifl ora. According to them, the general chieae of the family Primulaceae (CRONQUIST , form of pollen in Lysimachia is constant, the grains are TAKHTAJAN ) and is one of the largest genera in isopolar, tricolporate and they vary in size from smallest this family. Results of the recent phylogenetic studies of ( . × . μm in L. capillepsis) to medium (largest . primuloid families based on molecular and morphologi- × . μm in L. franchetii). cal data support the transfer of the genus Lysimachia to We have examined pollen morphology of Lysimachia Myrsinaceae (KÄLLERSJÖ et AL. , APG , MAR- vulgaris from natural habitats distributed all over Po- TINS et AL. , OH et AL. ). Genus Lysimachia land and from the cultivated population with the aim is divided into fi ve-six subgenera and many subgeneric of ( ) the characteristic of L. vulgaris pollen morphology sections (CHEN and HU , BENNEL and HU , AN- on the base of a large sample and ( ) the comparative DERBERG et AL. ). Lysimachia vulgaris belongs to the statistical analysis of the examined pollen traits in order subgenus Lysimachia and section Lysimachia (BENNEL and HU , HAO et AL. ). to evaluate L. vulgaris pollen grains diff erentiation. Lysimachia vulgaris is distributed in Europe, N Amer- ica, SW Asia and NW Africa (FERGUSON , INTER- NET http://www.eol.org). In Poland it is a common herb MATERIAL AND METHODS (ATLAS... ) occurring in moist habitats, such as fens, wet woods, lake shores, and river banks. It is a medicinal The examined pollen grains were collected from plant and the latest results of chemical studies show its natural Lysimachia vulgaris populations distributed all potential cytostatic and anticancer activity (UMEK , over Poland and from one cultivated population, which ŁUCZAK et AL. , JANIK et AL. , PODOLAK et AL. was treated by us as a preliminary attempt to fi nd out , PODOLAK and STRZAŁKA ). if there are diff erences in pollen morphology in de- Morphologically, Lysimachia is very heterogenous pendence of the plants growing conditions (Table ). with the variation in infl orescence structure, fl oral The cultivated population is located in the garden of morphology, pollination adaptation and growths habit. the Department of Botany of Poznań University of Life Studies of pollen morphology of species and varieties Sciences. It was started in from seeds collected D. Wrońska-Pilarek, M. Morozowska
TABLE . Location of studied pollen samples of Lysimachia vulgaris
Population number Localities Latitude (N) and longitude (E) Year of collection, herbarium