HPAI H2N8 finding in wild Mute (Cygnus olor) Sweden

Swedish Board of Agriculture

National Veterinary Institute, Sweden

2015-04-20 17/3 all 5 M-pos 18/3, 2 swans H5-pos 19/3 sequencing of cleavage site of HA -> HPAI Another 5 swans 19/3 H5N8 confirmed. found 18/2-9/3. Stored 5 swans were found 9/2- Sequencing of complete in freezer and send to 13/2, Autopsy and genome. Virus closely SVA 11/3-16/3 . analysis at SVA 9/2- related to strains (H5N8) Suspected 17/2. Confirmed lead circulating in EU. poisoning 4 swans, poisoning (post mortem 20/3 reporting OIE, traumatic injury 1 & chemical analysis) all ADNS swans. swan. All five AI negative

10 feb 20 feb 1 mars 10 mars 20 mars Location, central Stockholm

No commercial holdings in the vicinity

2015-04-20 A turkey Germany MV R2472 2014 A Netherlands emc 3 2014 A eurasian wigeon Netherlands emc 1 2014 A turkey Germany MV R2472 2014 A eurasian wigeon Netherlands emc 2 2014 A chicken Netherlands emc 3 2014 A turkey Italy 14VIR7898 10 2014 A turkey Germany NI R3372 2014 A eurasian wigeon Netherlands emc 1 2014 A England 36254 14 A turkey Germany R2474 L00899 2014 A eurasian wigeon Netherlands emc 2 2014 94 A Chicken Netherlands 14015526 2014 2014 Europe A turkey Italy 14VIR7898 10 2014

A chicken Netherlands 14015531 2014 - 88 A MuteSwan Sweden SVA1503110277 SZ05022015 2015 A turkey Germany NI R3372 2014

A MuteSwan Sweden SVA1503110141 SZ0543 2015 88 A duck England 36038 14 A duck England 36254 14 A duck England 36226 14 A turkey Germany R2474 L00899 2014 A wigeon Sakha 1 2014 2.3.4.4 Clade A spot billed duck Korea H455 42 2014 A Chicken Netherlands 14015526 2014 A Korea H411 2014 9893 A Korean native chicken Korea H257 2014 A chicken Netherlands 14015531 2014

A Baikal teal Korea H62 2014

A Coot Korea H81 2014 Koria South 88 A MuteSwan Sweden SVA1503110277 SZ0502 2015

A American green winged teal Washington 1957502014 2014 A MuteSwan Sweden SVA1503110277 SZ0543 2015 A Northern pintail Washington 40964 2014 100 Asian A gyrfalcon Washington 41088 6 2014 A duck England 36038 14

A turkey Washington 61 22 2014 -

99 A chicken Washington 61 9 2014 Hp A duck England 36226 14 -

A Washington 61 16 2014 H5

100 America North A wigeon Sakha 1 2014

A turkey BC FAV10 2014 2014 A duck Jiangsu k1203 2010

100 A herring Sweden V1116 06 - 2015 A tufted duck Sweden V599 06 0.0005

A mink Sweden V907 06

100 A goosander Sweden V539 06 A tufted duck Sweden V1027 06 A tufted duck Sweden V998 06 A eagle owl Sweden V1218 2006 Phylogenetic tree of HA gene of H5-AIV

A tufted duck Sweden V526 06 2.2.1 & 2.2 Clade

A eagle owl Sweden V618 2006 2006 Sweden based on 1000bp of heamagglutinin (HA1) A Sweden V978 06

A Sweden V827 06 gene. Phylogenetic tree was generated by

A smew Sweden V820 06 A duck Ireland 113 1983 the NJ method as implemented in MEGA 100 A Sweden 49 2002 80 software. The bootstrap test (2000 93 A mallard Sweden 40 2002 A mallard Sweden 64 2002 replicates) are shown next to the branches A mallard Sweden 21 2002 99 A mallard Sweden 101020 2009 96 A mallard Sweden 101224 2009 A mallard Sweden 74 2003 100 A mallard Sweden 7 2002

A mallard Sweden 39 2002 LP -

90 H5 Eurasian

0.01

A turkey Germany MV R2472 2014 A chicken Netherlands emc 3 2014 A eurasian wigeon Netherlands emc 1 2014 A turkey Germany MV R2472 2014 A eurasian wigeon Netherlands emc 2 2014 A chicken Netherlands emc 3 2014 A turkey Italy 14VIR7898 10 2014 A turkey Germany NI R3372 2014 A eurasian wigeon Netherlands emc 1 2014 A duck England 36254 14 A turkey Germany R2474 L00899 2014 A eurasian wigeon Netherlands emc 2 2014 94 A Chicken Netherlands 14015526 2014 2014 Europe A turkey Italy 14VIR7898 10 2014

A chicken Netherlands 14015531 2014 - 88 A MuteSwan Sweden SVA1503110277 SZ05022015 2015 A turkey Germany NI R3372 2014

A MuteSwan Sweden SVA1503110141 SZ0543 2015 88 A duck England 36038 14 A duck England 36254 14 A duck England 36226 14 A turkey Germany R2474 L00899 2014 A wigeon Sakha 1 2014 2.3.4.4 Clade A spot billed duck Korea H455 42 2014 A Chicken Netherlands 14015526 2014 A tundra swan Korea H411 2014 9893 A Korean native chicken Korea H257 2014 A chicken Netherlands 14015531 2014

A Baikal teal Korea H62 2014

A Coot Korea H81 2014 Koria South 88 A MuteSwan Sweden SVA1503110277 SZ0502 2015

A American green winged teal Washington 1957502014 2014 A MuteSwan Sweden SVA1503110277 SZ0543 2015 A Northern pintail Washington 40964 2014 100 Asian A gyrfalcon Washington 41088 6 2014 A duck England 36038 14

A turkey Washington 61 22 2014 -

99 A chicken Washington 61 9 2014 Hp A duck England 36226 14 -

A domestic duck Washington 61 16 2014 H5

100 America North A wigeon Sakha 1 2014

A turkey BC FAV10 2014 2014 A duck Jiangsu k1203 2010

100 A herring gull Sweden V1116 06 - 2015 A tufted duck Sweden V599 06 0.0005

A mink Sweden V907 06

100 A goosander Sweden V539 06 A tufted duck Sweden V1027 06 A tufted duck Sweden V998 06 A eagle owl Sweden V1218 2006 Phylogenetic tree of HA gene of H5-AIV

A tufted duck Sweden V526 06 2.2.1 & 2.2 Clade

A eagle owl Sweden V618 2006 2006 Sweden based on 1000bp of heamagglutinin (HA1) A Canada goose Sweden V978 06

A mute swan Sweden V827 06 gene. Phylogenetic tree was generated by

A smew Sweden V820 06 A duck Ireland 113 1983 the NJ method as implemented in MEGA 100 A mallard Sweden 49 2002 80 software. The bootstrap test (2000 93 A mallard Sweden 40 2002 A mallard Sweden 64 2002 replicates) are shown next to the branches A mallard Sweden 21 2002 99 A mallard Sweden 101020 2009 96 A mallard Sweden 101224 2009 A mallard Sweden 74 2003 100 A mallard Sweden 7 2002

A mallard Sweden 39 2002 LP -

90 H5 Eurasian

0.01