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STUDIES ON THE ARTHROPOD FAUNA OF ALFALFA V. SPIDERS (ARANEIDA)' A. G. WHEELER,JR.~ Department of Entomology, Cornell University, Ithaca, New York Abstract Can. Ent. 105: 425-432 (1973) The spider fauna of alfalfa was studied at Ithaca, N.Y., in three experimental plots and in nearby fields during 1966-69. As part of an overall study of the arthropod fauna of alfalfa, the spider populations in the study plots were recorded weekly during visual examination of the terminal 6 in. of five stems for 100 plants in each of the plots. Additional information was obtained from sweep-net and pitfall trap collections in the study plots and from sweeping several commercial plantings. The spider fauna of alfalfa was found to consist of 78 species, 10 of which were collected only from the ground layer. Tetragnatha 1abo:iosa Hentz and Dictyna volucripes Keyserling were the most abundant species found in the weekly samples. Notes on the seasonal occurrence, location on the plants, and feeding habits are given for the common species. Introduction Although the spiders associated with various specialized habitats, e.g., cedar glades (Meyer 1937), xeric dunelands (Lowrie 1942), and maritime communities (Barnes 1953; Barnes and Barnes 1954), have been studied, it has been only recently that the spiders associated with crop plants have received the attention they deserve. Among the more detailed studies on crop plant spiders are those on cotton by Whitcomb et al. (1963) and Whitcomb and Bell (1964) and on grain sorghums by Bailey and Chada (1968). Several lists of spiders found in crop plants have been compiled. Spiders occurring in corn were reported by Everly (1938), those in cole crops by Pimentel (1961 ), those in sugar cane by Hensley et al. ( 1961) and those in soybeans by Blickenstaff and Huggans (1962). Alfalfa, Medicago sativa L., has been noted to harbor an extremely rich arthropod fauna (Folsom 1909) and an unusually large general population of spiders (Muniappan and Chada 1970). However, there had been no compre- hensive survey of the spider fauna of alfalfa until the recent survey of Virginia alfalfa fields by Howell and Pienkowski ( 1971 ) . Previous to their work, Fenton (1959) mentioned that 15 species of spiders were taken from alfalfa in Oklahoma; those species plus two additional ones were listed by Fenton (ms.). The primary purpose of this investigation, which is part of an overall study of the arthropod fauna of alfalfa, was to determine the species composition of the spider fauna occurring in alfalfa fields at Ithaca, N.Y. Additional aims were to determine the position of the various species on the plants, their feeding habits, and their relative abundance in the alfalfa community. Methods The majority of the spider collections and observations were made at Cornell University's Savage Farm during visual examination of alfalfa foliage as part of the systematic sampling of the alfalfa-arthropod community. Sampling consisted of the weekly counting of all arthropods on the terminal 6 in. of five stems on 100 plants in three study plots 100' x 100'. Details of the sampling technique and plots can be found in Pimentel and Wheeler (in press). Additional collections of spiders were made by sweeping alfalfa in the study plots and in other alfalfa fields 'This research supported by grant GB-19239 from the National Science Foundation. =Present address: Bureau of Plant Industry, Pennsylvania Department of Agriculture, Harrisburg. 425 Downloaded from https://www.cambridge.org/core. Tufts Univ, on 24 Mar 2018 at 18:52:38, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.4039/Ent105425-3 426 THE CANADIAN ENTOMOLOGIST March 1973 Table I. The spider fauna of alfalfa fields at Ithaca, N.Y., giving the stages collected on the plants and in the ground layer and overwintering within the fields (A, adult; S, subadult; I, immature) Stages Stages collected collected Overwinteringm Species on plant on ground stages Theridiidae Argyrodes trigonum (Hentz) I Enoplognatha ovata (Clerck) A Theridion differens Emerton A, I T.frondeum Hentz A, I T. murarium Emerton I Thymoites unimaculata (Emerton) A, I Linyphiidae Bathyphantes concolor (Wider) A, I Centromerus cornupalpis (0. P. - Cambridge) Ceraticelus emertoni (0. P. - Cambridge) A C. similis (Banks) A Ceratinopsidis formosa (Banks) A Eridantes erigonoides (Emerton) A Erigone autumnalis Emerton I E. blaesa Crosby & Bishop A, I Hypselistes $orens (0.P. - Cambridge) A, I Grammonota inornata Emerton A G. pictilis (0. P. - Cambridge) A Linyphia mandibulata Emerton A, 1 L. waldea Chamberlin and Ivie Meioneta nigripes (Simon) M. sp. A M. sp. B Tennesseellum formicum (Emerton) Walckenaera vigilax (Blackwall) Araneidae Acanthepeira stellata (Walckenaer) Araneus marmoreus Clerck A. trijolium (Hentz) A. sp. (cavaticus type) Araniella displicata (Hentz) Argiope trifasciata (Forskal) Cyclosa sp. Eustala cepina (Walckenaer) Larinia sp. Mangora gibberosa (Hentz) Mimoguatha foxi (McCook) Nesoscona arabesca (Walckenaer) Pachv~nathatristriata C. L. Koch 5'ingaratensis Emerton Tetragnatha laboriosa Hentz Mimetidae Mimetus epeiroides Emerton I Agelenidae Agelenopsis sp. I Pisauridae Dapanus miris (Walckenaer) A Lycosidae ? Arctosa sp. Lycosa helluo Walckenaer Pardosa milvina (Hentz) P. nzodica (Blackwall) P. moesta Banks Pirata minutus Emerton Downloaded from https://www.cambridge.org/core. Tufts Univ, on 24 Mar 2018 at 18:52:38, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.4039/Ent105425-3 Volume 105 THE CANADIAN ENTOMOLOGIST Table I. (Continued) Stages Stages collected collected Overwintering Species on plant on ground stages Clubionidae Anyphaena sp. Anyphaenella saltabunda (Hentz) Chiracanthium SD. Clubiona abbotii'l. Koch C. obesa Hentz I Trachelas tranquillus (Hentz) A Thomisidae Misumena calycina (Clerck) Misumenoides aleatorius (Hentz) Mzsumenops asperatus (Hentz) Philodromus placidus Banks P. rufus Walckenaer Synema sp. Tibellrts oblongrts (Walckenaer) Tn~nrlcsangulntits (Il'alckenaer) -yslicrrs disrrtrsnns Keyserling S.,funfsks Keywrling X.~uroslis Keyserling S,luclnns (C. L. 1Cot.h) Salticidae Hubronattus decorus (Blaclcc~all) I A Icizcr lanrfii (Emerton) I dIetapkidipp~rsgaktkca (Kalcke~laer) A, 1 AT. pralcrvur (if aIckenacr) I Phidipims ntidas (Hentz) S P. princcps (Peckham) I I Talrrz*ern n~inrlla (Banks) A Dictynidae Argenna? obesa Emerton I Dictyna foliacea (Hentz) A D. hentzi Kaston A, I D. manitoba Ivie A D. volucripes Keyserling A. I I in the Ithaca, N.Y., area. The spider fauna of the ground layer was determined through pitfall trap collections taken in the study plots. Results Of the 78 species collected in alfalfa, 45 (57.7% ) were web builders and 33 (42.3%) were hunters. Excluding the 10 species that were taken only from the ground layer, 40 species (58.8% ) were web builders and 28 species (41.2%) were hunters. Table I lists the 78 species and their stages, i.e., adults or immatures, taken on foliage and the ground layer, and the overwintering stages found within the fields. Notes on individual species, including location on the plants, feeding habits, and seasonal occurrence, are presented by family in the following discussion. Theridiidae. Members of the Theridiidae were never abundant on alfalfa. Theridion murarium, the most numerous of the theridiids, built a tangled web on terminal buds and was found on the plants from early May to late October. Eno- plognatha ovata also built a loose, tangled web on terminal stems and was also found occasionally in webs on stems near the soil surface. During July, females with egg sacs were found concealed among rolled leaflets near the top of the plants. A Downloaded from https://www.cambridge.org/core. Tufts Univ, on 24 Mar 2018 at 18:52:38, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.4039/Ent105425-3 428 THE CANADIAN ENTOMOLOGIST March 1973 female of T. differens was also found in July with an egg sac concealed within four leaflets tied together with silk. Linyphiidae. In terms of the number of species this was the dominant family of spiders, but few of the species were abundant in alfalfa. Linyphia mandibulata, however, was one of the most common spiders found in the study. It built a dense, flat, platform web among stems, usually several inches below the top of the plant. During April, immatures were found in small webs at the base of the plants. L. mandibulata was observed to feed on large numbers of potato leafhoppers, Empoasca fabae (Harris), as well as on clover leafhoppers, Aceratagallia sanguinolenta (Provancher), and small Diptera. Tennesseellum formicum was commonly found in webs at the base of small plants. Collembola and pea aphids, Acyrthosiphon pisum (Harris), frequently were caught in the webs. Immatures of T. formicum were also found wandering over foliage, and adults and immatures were taken in pitfall traps. Bathyphantes concolor built a sheet web near the base of the plants. In late October 1969 it was collected in large numbers on the plants, along with Walckenaera vigilax, following heavy frost. The ballooning habit following the first frosts of autumn is well known among the Linyphiidae (Kaston 1948). Erigone blaesa built small irregular webs on stems near the ground; immatures were also taken on the soil surface. An egg sac of E. blaesa was found in a crevice in the soil in mid-July. Hypselistes florens built small irregular webs on stems near the ground; it also was observed to hunt over the foliage and on one occasion to capture a pea aphid. Araneidae. Several orb weavers were among the most abundant spiders in alfalfa. Tetragnatha laboriosa, the most abundant species found on the plants, was most numerous from June to October. It built symmetrical webs near the top of plants and was observed to feed on potato and clover leafhoppers, cowpea aphids, Aphis craccivora Koch, tarnished plant bugs, Lygus lineolaris (Beauvois), the anthocorid Orius insidiosus (Say), and small Diptera.