EIF3D Antibody Cat. No.: 19-956
EIF3D Antibody
Immunofluorescence analysis of L-929 cells using EIF3D Immunofluorescence analysis of C6 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining. nuclear staining.
Immunofluorescence analysis of L-929 cells using EIF3D Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining. nuclear staining.
Specifications
HOST SPECIES: Rabbit
September 30, 2021 1 https://www.prosci-inc.com/eif3d-antibody-19-956.html SPECIES REACTIVITY: Human, Mouse, Rat
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of IMMUNOGEN: human EIF3D (NP_003744.1).
TESTED APPLICATIONS: IF, IHC, WB
WB: ,1:200 - 1:2000
APPLICATIONS: IHC: ,1:50 - 1:200
IF: ,1:50 - 1:200
POSITIVE CONTROL: 1) SGC-7901
2) U-87MG
3) HeLa
4) NIH/3T3
5) Mouse liver
6) Rat testis
PREDICTED MOLECULAR Observed: 64kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: EIF3D
ALTERNATE NAMES: EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta, EIF3S7
GENE ID: 8664
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
September 30, 2021 2 https://www.prosci-inc.com/eif3d-antibody-19-956.html Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It BACKGROUND: is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 30, 2021 3 https://www.prosci-inc.com/eif3d-antibody-19-956.html