EIF3D Antibody Cat. No.: 19-956

EIF3D Antibody

Immunofluorescence analysis of L-929 cells using EIF3D Immunofluorescence analysis of C6 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining. nuclear staining.

Immunofluorescence analysis of L-929 cells using EIF3D Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining. nuclear staining.

Specifications

HOST SPECIES: Rabbit

September 30, 2021 1 https://www.prosci-inc.com/eif3d-antibody-19-956.html SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion containing a sequence corresponding to amino acids 1-240 of IMMUNOGEN: human EIF3D (NP_003744.1).

TESTED APPLICATIONS: IF, IHC, WB

WB: ,1:200 - 1:2000

APPLICATIONS: IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

POSITIVE CONTROL: 1) SGC-7901

2) U-87MG

3) HeLa

4) NIH/3T3

5) Mouse liver

6) Rat testis

PREDICTED MOLECULAR Observed: 64kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: EIF3D

ALTERNATE NAMES: EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta, EIF3S7

GENE ID: 8664

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 30, 2021 2 https://www.prosci-inc.com/eif3d-antibody-19-956.html Eukaryotic -3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It BACKGROUND: is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this is the major RNA binding subunit of the eIF3 complex.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 30, 2021 3 https://www.prosci-inc.com/eif3d-antibody-19-956.html