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Programm Phage Meeting Berl IMPRINT CONTENT EDITED BY PROGRAM ...................................................... 4 Christian G. Willy, MD, PhD Monday 16.12.2019 ................................................ 4 Professor of Surgery Tuesday 17.12.2019 ................................................ 7 Colonel Clinical Director Department Trauma & Orthopedic Surgery, Septic & Reconstructive Surgery Research and Treatment Center for Complex Combat Injuries INTRODUCTION .................................................. 10 Wound Center (ICW) FACULTY ....................................................... 11 Bundeswehr (Military) Academic Hospital Berlin Scharnhorststr. 13 10115 Berlin OPEN QUESTIONS ................................................ 60 Germany Production of Single Phage Solutions ..................................... 60 Tel: +49 (0) 30 2841 1900 (direct line) Production of the individual Phage Cocktail ................................. 60 +49 (0) 30 2841 1901 (offi ce) Magistral Production vs. “Fixed Cocktails” ................................. 61 Fax: +49 (0) 30 2841 1909 Cell: +49 (0) 179 940 81 33 Synthetic phages with programmable specifi city .............................. 61 E-mail: [email protected] Measurement of Susceptibility .......................................... 61 E-mail: [email protected] Biofi lm ........................................................ 61 Prevention ...................................................... 62 Phage Application ................................................. 62 Phage Treatment, Immunoreaction ....................................... 62 Combination with Antibiotic therapy ...................................... 63 Phage isolation, Phage Bank and Exchange of Phages ........................... 63 Networking ..................................................... 63 Scharnhorststraße 1 BWK BERLIN ANSWERS TO OPEN QUESTIONS ....................................... 64 A. Production of Single Phage Solutions ................................... 64 B. Production of the individual Phage Cocktail ................................ 74 C. Magistral Production vs. “Fixed Cocktails” ............................... 75 D. Synthetic phages with programmable specifi city ............................. 78 Kieler Straße E. Measurement of Susceptibility ........................................ 80 F. Biofi lm ....................................................... 83 Ida-von-Arnim-Straße G. Prevention .................................................... 85 H. Phage Application ............................................... 86 2 I. Phage Treatment, Immunoreaction ..................................... 91 J. Combination with Antibiotic therapy .................................... 94 K. Phage isolation, Phage Bank and Exchange of Phages ......................... 95 1 Entrance Bundeswehr (Military) Hospital Berlin, Scharnhorststr. 13 - Berlin-Mitte L. Networking .................................................... 98 2 Restaurant “ESSZIMMER”, Scharnhorststraße 28-29 - Berlin-Mitte 1 © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, Mil Acad Hosp Berlin, DSMZ, FH-ITEM 2 3 Mil Acad Hosp Berlin, DSMZ, FH-ITEM MONDAY, 16th DECEMBER 2019 MONDAY, 16th DECEMBER 2019 REGISTRATION 08:00 - 08:30 EFFORTS FOR AN OFFICIAL APPROVAL Chairs: Kutateladze (GEO), Schooley (USA), Pirot (FRA) 13:15 - 13:33 Young (USA) Towards a phage therapy that is safe, scientifi cally sound, and sustainable WELCOME 13:33 - 13:40 Brake (GER) Defi ning a regulatory framework for phage therapy - 08:30 - 08:35 Becker (GER) A warm welcome to you what is desirable, what is possible and what can be achieved 08:35 - 08:45 Rottmann-Grossner (GER) Phage Therapy for the Health System in Germany (Europe) 13:40 - 13:52 Ceyssens (BEL) Magistral Phage: The public health approval of active phage ingredients of the magistral preparation 08:45 - 08:50 Willy (GER) The big line for this meeting 13:52 - 14:04 Clokie (UK) Mapping clinical need to phage therapy treatments to address regulatory hurdles in the UK 14:04 - 14:16 Debarbieux (FRA) Current situation in France for Phage treatment PHAGE TREATMENT 14:16 - 14:30 All Comments, Questions, Discussion Chairs: Chanishvili (GEO), Metsemakers (BEL), Kutter (USA) 14:30 - 15:00 Break 08:50 - 09:08 Kutateladze (GEO) Phage therapy in Georgia - Update 2020 08:08 - 09:26 Kutter (USA) A surgical look at the history of phage therapy PRODUCTION OF PHAGE SOLUTIONS 08:26 - 09:44 Schooley (USA) Treatment with parenteral and aerosolized phage Chairs: Ziehr (GER), Ceyssens (BEL), Deng (GER) preparations and dose ranging study of the antimicrobial activity of a fi xed anti-pseudomonas phage cocktail 15:00 - 15:12 Pirot (FRA) The pharmaceutical practice in Bacteriophage therapy - 09:44 - 09:56 Ascherl (GER) Phages in recurrent periprosthetic joint infection - Stability control of the phage suspension, phage counting, ... Own experiences since 2015 15:12 - 15:24 Mölling (GER) Bacterial and phage composition after fecal microbiota 09:56 - 10:08 Rümke (GER) The Hannover experience in phage treatment transplantation in a patient with C. diffi cile 10:08 - 10:20 Kolesnyk (UKR) Phage treatment in Ukraine 15:24 - 15:36 Deng (GER) Towards systematic phageome therapy: cultivation-free phage illustration, single-phage isolation and cell-free synthesis 10:20 - 10:30 Gabard (FRA) Bone/joint, prosthesis, dura matter infections: case studies 15:36 - 15:48 Kiljunen (FIN) Finnish experience on setting up the phage production and 10:30 - 11:00 Break purifi cation - Status quo and perspectives 11:00 - 11:12 Leitner (CH) Bacteriophages for treating urinary tract infections 15:48 - 16:00 Rubalski (GER) Phage production, purifi cation and local drug delivery systems in patients undergoing transurethral resection of the prostate: a randomized, placebo-controlled, double-blind clinical trial 16:00 - 16:10 Wienecke (GER) Phage GMP-Manufacture – Initial approach 11:12 - 11:24 Miedzybrodzki (POL) Phage therapy 2019 – An update of the Polish experience 16:10 - 16:22 Sicheritz-Pontèn (DEN) What are the benefi ts of AI, machine learning in the discovery of phages? 11:24 - 11:42 Debarbieux (FRA) Tripartite interactions between bacteriophages, bacteria and immune cells during pulmonary phage therapy 16:22 - 16:34 Leitner (CH) Engineered bacteriophages as alternative for antibiotic treatment for catheter associated urinary tract infections 11:42 - 11:54 Clokie (UK) Prophylactic use of bacteriophages to prevent bacterial infection 16:34 - 16:46 Bugert (GER) Extension of therapeutic phage host range: identifi cation of depolymerases for the lysis of different K pneu capsule types 11:54 - 12:25 All Comments, Questions, Discussion 12:25 - 13:15 Break © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, Mil Acad Hosp Berlin, DSMZ, FH-ITEM 4 5 Mil Acad Hosp Berlin, DSMZ, FH-ITEM MONDAY, 16th DECEMBER 2019 TUESDAY, 17TH DECEMBER 2019 PRODUCTION OF PHAGE SOLUTIONS CONCLUSIONS DAY 1 Chairs: Ziehr (GER), Ceyssens (BEL), Deng (GER) 08:00 - 08:30 All Conclusions of the task groups I-III 16:46 - 17:05 All Comments, Questions, Discussion 08:30 - 08:35 All Comments, Questions, Discussion 17:05 - 17:10 Location change RESEARCH PROJECTS Chairs: Young (USA), Witzenrath (GER), Chanishvili (GEO) TASK GROUP I Chairs: Miedzybrodzki (POL), Stengel (GER), Schooley (USA) 08:35 - 08:50 Pirnay (BEL) Phage therapy research at the Queen Astrid Military Hospital (QAMH) 17:10 - 18:25 Part of All What is the evidence for the benefi t of phage therapy today? 08:50 - 09:05 Gabard (FRA) Phagoburn - Experiences and necessary implications for further studies 09:05 - 09:10 Stichling (GER) PhagoFlow, Germany TASK GROUP II 09:10 - 09:20 Witzenrath (GER) Phages, lysins and Phage4Cure: towards treatment for Chairs: Ceyssens (BEL), Clokie (UK), Brake (GER) respiratory tract infections 09:20 - 09:30 Michael (USA) Infectious Diseases Countermeasure Development in Support 17:10 - 18:25 Part of All The challenge: Which quality standards are required for of Multi Domain Operations - The Walter Reed Army Institute phage pharmaceutical products? of Research efforts 09:30 - 09:50 Swierczewski (USA) The US Army Bacteriophage Therapeutics Program for the Treatment of Multidrug Resistant Wound and Infections TASK GROUP III 09:50 - 10:10 All Comments, Questions, Discussion Chairs: Debarbieux (FRA), Swierczewski (USA), Witzenrath (GER) 10:10 - 10:40 Break 17:10 - 18:25 Part of All How do we fi nance RCTs, and which indications should be targeted fi rst? “PHAGOGRAM”, “TO FIND THE RIGHT PHAGE” Chairs: Sicheritz-Pontèn (DEN), Di Luca (ITA), Clokie (UK) 19:30 - — Dinner Restaurant “ESSZIMMER” (Scharnhorststraße 28-29), http://www.esszimmer-berlin.de/ 10:40 - 10:52 Korf (GER) On the way to a phagogram 10:52 - 11:04 Skurnik (FIN) An easy-to-use phage typing system 11:04 - 11:16 Sicheritz-Pontèn (DEN) Possibilities of prediction of host relations and killing effi cacy of isolated phages - Usefulness of machine learning in antiseptic medicine 11:16 - 11:30 All Comments, Questions, Discussion 11:30 - 11:35 Session change © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, © 2019 - Dep of Trauma Ortho Reco and Plastic Surgery, Mil Acad Hosp Berlin, DSMZ, FH-ITEM 6 7 Mil Acad Hosp Berlin, DSMZ, FH-ITEM TUESDAY, 17TH DECEMBER 2019 TUESDAY, 17TH DECEMBER 2019 BIOFILM & COMBINATION WITH ANTIBIOTIC THERAPY TASK GROUP II Chairs: Azeredo (POR), Debarbieux (FRA), Skurnik (FIN) Chairs: Schooley (USA), Leitner (CH), Bugert
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