Anti-PSME4 Antibody (ARG40059)

Product datasheet [email protected]

ARG40059 Package: 100 μl anti-PSME4 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes PSME4

Tested Reactivity Hu, Ms, Rat

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name PSME4

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 1634-1843 of Human PSME4 (NP_055429.2).

Conjugation Un-conjugated

Alternate Names PA200; Proteasome activator complex subunit 4; Proteasome activator PA200

Application Instructions

Application table Application Dilution

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control Rat liver, Mouse heart and MCF7

Calculated Mw 211 kDa

Observed Size 211 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Gene Symbol PSME4

Gene Full Name proteasome activator subunit 4

Function Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt]

Cellular Localization Cytoplasm, cytosol. Nucleus. Nucleus speckle. Note=Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2)O(2). [UniProt]

Images

ARG40059 anti-PSME4 antibody WB image

Western blot: 25 µg of Rat liver, Mouse heart and MCF7 cell lysates stained with ARG40059 anti-PSME4 antibody at 1:3000 dilution.