Anti-GBP1 Antibody Catalog # ABO10867

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Specification

Anti-GBP1 Antibody - Product Information

Application WB Primary Accession P32455 Host Rabbit Reactivity Human Clonality Polyclonal Format Lyophilized Description Rabbit IgG polyclonal antibody for Guanylate-binding protein 1(GBP1) detection. Tested with WB in Human.

Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Anti-GBP1 antibody, ABO10867, Western Anti-GBP1 Antibody - Additional Information blottingAll lanes: Anti GBP1 (ABO10867) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at Gene ID 2633 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at Other Names 40ugPredicted bind size: 68KDObserved bind Guanylate-binding protein 1, 3.6.5.-, size: 68KD GTP-binding protein 1, GBP-1, HuGBP-1, Guanine nucleotide-binding protein 1, Interferon-induced guanylate-binding Anti-GBP1 Antibody - Background protein 1, GBP1 Interferons induce a large number of genes in Calculated MW their target cells, including those coding for 67931 MW KDa guanylate-binding proteins(GBPs). GBPs, such as GBP1, are characterized by their ability to Application Details specifically bind guanine nucleotides(GMP, Western blot, 0.1-0.5 µg/ml, Human
GDP, and GTP) and are distinguished from the Subcellular Localization GTP-binding proteins by the presence of 2 Cytoplasm. Golgi apparatus membrane; binding motifs rather than 3. The 593-amino Lipid- anchor; Cytoplasmic side. Secreted. acid GBP1 protein shares 77% and 88% Secreted from endothelial cells in the identity with GBP2 and GBP3, respectively. All cerebrospinal fluid, upon bacterial GBPs, including GBP1, have a conserved challenge and independently of N-terminal globular GTP-binding domain interferon-gamma induction. Golgi containing 2 consensus sequences and a third membrane localization requires T(L/V)RD sequence not found in other GTPases. isoprenylation and the presence of another The GBP1 gene to the GBP gene cluster on IFN-gamma-induced factor. chromosome 1p22.2. It is located telomeric to GBP2 and centromeric to GBP3. High Protein Name expression of GBP1, GBP2, and GBP3 are in Guanylate-binding protein 1 endothelial cells after stimulation with IFNG,

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Contents TNF, or IL1B . Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GBP1(566-589aa QKESRIMKNEIQDLQTKMRRRKAC).

Purification Immunogen affinity purified.

Cross Reactivity No cross reactivity with other proteins

Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. Sequence Similarities Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Anti-GBP1 Antibody - Protein Information

Name GBP1

Function Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.

Cellular Location Cytoplasm. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cell membrane Secreted. Note=Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor

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Anti-GBP1 Antibody - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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