St. Paul's Hospital Accessioning Test Reference

St. Paul’s Hospital Accessioning Test Reference

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

1,25-dihydroxyvitamin D VITD 1 RED top Optimal 2 mL, minimum 0.6 mL Red top serum. Separate from monthly SPH Special Chem cells and freeze. Send on ice pack. Stability: 7 days at room Calcitriol (Do not use gel temp and 2-8°C, 2 months at -20°C. 1,25- tubes) Dihydroxycholecalciferol Test done once a month. 1,25-Dihydroxy-Vit-D, ***Double Check Order*** Use VITD3 if req says “VIT D” or “Vitamin D”

4 Amino Pyridine NCODE 1 SST Gold top 1 mL serum. Freeze. Ship in dry ice. Y Att. Dr. James Lesley University Of Maryland Information outdated, need to be verified. 100 Penn St. Room 540 Baltimore Maryland 212001 U.S.A

7-Dehydrocholesterol DEHYD Amniotic fluid Refer Outpatients to Children’s Hospital for amniotic fluid Y Children’s Hospital collection. Send copy of Cytogenetics requisition with transport Sterol, Lathosterol, Sterols, RED? batch. Desmosterol, SLOS, Smith Lemli Opitz Syndrome, Dehydrocholesterol Hospitals In-Common Laboratory 11-Deoxy-Cortisol NCODE 1 SST Gold top or 1 mL serum. Store and send frozen on dry ice. 7 days Y Inc. PST Light green Additional information on HICL website: 57 Gervais Dr. top http://www.HICL.on.ca/search_tcna.asp?Tcstring=11DEOXY North York, ON M3C 1Z2 Phone 416-391-1499 Ext.248 Fax 416-385-1957 17-Hydroxycorticosteroids Do urine Cortisol

17-Hydroxyprogesterone OHP 1 RED Top 1 mL RED Top serum. Freeze. Send on ice pack. Children’s Hospital Lab

17 Ketogenic-Steroids Do urine Cortisol

17 Ketosteroids Unavailable. Bill and collect Serum for DHEA-Sulfate and free testosterone

2-3 DPG Not available as of MAY01/08

Division of Pre and Post Examination, Page 1 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

2,3-Dinor-11Beta-Prostaglandin NCODE 24-hour urine Must have MSP Approval letter prior to collection. Y Specimen Process Centre F2 Alpha, Urine collection Mayo Medical Laboratories CRU 1. Ask patient to sign Bill73 consent form. 3050 Superior Drive NW Mayo Test ID: 23BPG No preservative UTIM 2. Instruct patient to refrigerate urine during 24 hour collection. Rochester, MN 55901 preferred.(see Mayo for other Accessioning: process 24 hour urine: 1-800-533-1710 preservatives). 1. 1 aliquot for CRR 2. 1 aliquot for 23 BPG, spun and aliquot 4 mL, freeze immediately. Stability: Refrigerated 7 days, Frozen 30 days, Ambient 8 hours SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor, provide total 24 hour volume in Comments section of MayoLink Test orders.: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Send frozen on dry ice. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.).

3,4- DRSCB 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Methylenedioxyamphetamine Sunquest LIS Order Entry: >2mL Urine Second screen: DRSC1 =;3,4 Methylenedioxyamphetamine

5, 1 Nucleotidase (5,1 NCODE 1 SST Gold top 2.0 mL serum. 0.5 mL minimum. Ship Serum frozen. Y Quest Diagnostics/ Nichols Institute NTD)(5NTD) Mandatory MSP pre-approval required for Outpatients 33608 Ortega Highway (request to be done by patient Dr.). For Outpatients, do not San Juan, Capistrano, collect without MSP preapproval if test is ordered alone. CA 92675 Complete Quest Diagnostics’ Test Request Form and Put Fax 1(800) 553-5445 Result to SPH Lab request sticker Only done on 24 hr samples. Random is unavailable. Adjust 24 5HIAA (5-Hydroxy Indole Acetic HIAAU 24 hour urine VGH Lab hour collection pH to 2 - 3 and aliquot 50 mL of a well-mixed 24hr Acid) collected in 6 M Add CRU, collection. Refrigerate. Send on ice pack. (Serotonin. HCl, refrigerate UTIM For 48 h before and during collection, patients should not 5-Hydroxytryptamine - 5HT) during collection. consume foods high in serotonin such as avocados, bananas, butternuts, cantaloupe, dates, eggplant, grapefruit, hickory nuts, honeydew melon, kiwifruit, melons, nuts, pineapple, plantains, plums, tomatoes. Medications, such as L-dopa, acetaminophen, salicylates, and cough syrup containing guaifenesin must also be avoided.

Division of Pre and Post Examination, Page 2 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Abnormal Pigments Phone ordering physician for specific orders

ABO Antibody Titres ABOAT Two 6mL LAV top Confirm with Transfusion Medicine that testing has been 2-4 SPH Transfusion Medicine (Blood or 2 RED top arranged. Hours Bank) Mon-Fri, Routine: ABO/Rh Type ABR 6 mL LAV top Deliver copy of requisition with specimen to Transfusion SPH Transfusion Medicine (Blood 1-3 hrs Medicine Bank)

STAT: 1 hr 24 hours

ACL (Anti-Cardiolipin Antibody, ACA 1 SST Gold top 1.0 mL serum. Freeze Serum. Send on ice pack. VGH Lab Anti Cardiolipin Ab) Avoid hemolysis >2.90

Acebutolol ACEBB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Acetazolamide ACTZB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre (Diamox) requisition for send out.

Division of Pre and Post Examination, Page 3 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Acetyl-Cholinesterase Unavailable. (See Pseudocholinesterase)

Acetylcholine Receptor ACETRB 1 SST Gold top 2 mL serum. Refrigerate Serum and send on ice pack. Copy of 1 week UBC Lab via VGH Lab Antibodies (Choline- requisition for sendout. 604-822-7175 Receptor Antibody) Anti-Musk Ab: Order NCODE1 along with ACETR. Copy of (Myasthenia Gravis requisition for UBC Lab. Put Fax Result to SPH Lab request Evaluation; MG antibodies; sticker on requisition or on Sunset Printout. Myoid Antibody)

Acid Glycerol Lysis Test Not available

Division of Pre and Post Examination, Page 4 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Acid Lipase, Lymphocyte ALIPB NBS Card Optimal: 4 spots, Minimum: 1 spot. Y Children’s Hospital – Biochemical Genetics Lab Acid Lipase, bloodspot Preferred: Collect using syringe and drip blood on blood spot 604-875-2307 Acid esterase, Wolman card. disease; cholesteryl ester Acceptable: storage disorder Collect 1 – 2 mL EDTA blood and transfer to blood spot card. EDTA blood must be transferred to blood spot card within 6 hrs of collection. If using finger/heel prick, allow blood to drip rather than touching blood spot card with patient's finger/heel.

Obtain minimum one completely filled circle that is soaked through the back of card.

Copy of requisition for Approval. Identify name of test on blood spot card. Allow blood spots to dry completely on flat surface for minimum 4 hrs. Do not expose to heat or direct sunlight. Once dry, place blood spot card in sealed plastic bag with a sachet of desiccant (if available). Store in 4°C if there is delay in shipping. Ship at room temperature by overnight courier to Specimen Receiving 2J20. Inform lab at 6048752307 to expect the sample.

Acidified Serum Test (Also HAM 1 LAV top and See Hematology prior to collecting. Photocopy of requisition to Y SPH – Hematology Called Ham’s Test; Acid 1RED top tube for Hematology. Hemolysis Test) Normal Control. Test no longer available as of March 2017

Division of Pre and Post Examination, Page 5 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Acylcarnitine Profile, Bloodspot ACYDOT Blood spot 4 blood spots on Blood Dot Card (NBS Newborn Screening 1 week Y Children’s Hospital – Biochemical Whatman 903 NBS Filter card), minimum 2 spots. Genetics Lab Whatman 903 NBS Room 2F22 card (Newborn Collection: Screening card, 604-875-2307 Preferred: Use syringe and needle method: collect 2 mL in to Blood Dot Card) Syringe, activate safety device on needle. Remove needle. Apply

blood immediately to blood spots. Use Butterfly with syringe assembly if other blood work is collected at the same time. Alternate method: Collect 3mL EDTA tube with needle and holder. Apply blood to blood spots with transfer pipette. Do NOT use the pneumatic tube system to transport wet bloodspot cards. Wet bloodspot cards must NOT be packaged in biohazard bags. Complete patient information on Blood Dot Card, write Acylcarnitine Profile on card. Allow blood dots to air dry for at least 3 hours. Form Transport Batch and send separately, do not batch with other NBS cards. Send Card at room temp. Copy of requisition for approval.

Acylcarnitine, Serum ACYSER 1 RED top 1.0 mL Red top serum preferred. Minimum 0.5 mL. Freeze and 8 weeks Y Children’s Hospital – Biochemical (Dr must specify serum test, ship frozen on dry ice the same day or overnight. Genetics Lab otherwise order Bloodspot Test) Copy of requisition for approval. Room 2F22

ACTH Stimulation Test CORF 1 SST Gold top Patient given ACTH (Cosyntropin) to stimulate adrenal glands. Mon-Fri SPH Special Chemistry Lab (cosyntropin test) Cortisol is measured before (baseline) and at 30 min and 60 min COR30 post. COR60

ACTH, Inferior Petrosal Sinus ACTHPS 1LAV top on ice Collect on ice, deliver to Lab ASAP. 1.0 mL plasma in False Mon. SPH Special Chemistry Lab Sampling Bottom or 13 x 75 Polypropylene tube, freeze immediately. Thurs. Send frozen on dry ice. Specimen must arrive @ SPH frozen. Copy of requisition for Special Chemistry. Order Entry: free text Sample Timing and Site info in Modifier field and result ACTHTG, IPSITE (second screen). e.g. ACTHTG = ;Baseline or ;10 min post etc…. IPSITE = ;Peripheral vein or ;Left inferior petrosal sinus. etc…

Division of Pre and Post Examination, Page 6 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Double spin all tubes and separate serum/plasma into 5 VGH Autoimmune Lab ADAMTS13 Activity and ADM13B 2 BLUE top citrate Once per Y polypropylene tubes. Freeze all 5 tubes within 2 hours of Inhibitor Profile month and collection. Send frozen on dry ice. ADAMTS13 Antibody 2 RED top Minimum volume 2 mL each EDTA and citrate plasma and 4 mL von Willebrand Factor and serum Cleaving Protease 6.0 mL EDTA

All tubes Double Spun 3 mL RED top whole blood. Samples must be sent, on ice pack, Children’s Virology Adenovirus PCR CADVL 1 RED top Y Monday to Friday 800 to 2300 or Saturday, Sunday and Statutory (blood) Holidays 800 to 1600. Specimen must be received within 48 hours of collection. Adenovirus PCR PVPCR 1 mL urine or Refrigerate. Mon – SPH Virology Fri (non-blood) swabs, body Result in fluids, etc… 2 days

Adrenocorticotropic Hormone ACTH 1 LAV on ice 1.0 mL EDTA Plasma collected on ice, centrifuge at 4°C, stored Twice SPH Special Chemistry Lab and sent frozen on dry ice. Specimen must arrive @SPH frozen. per week

AFB Fluid Culture PTBF Fluid in sterile 8 weeks SPH Microbiology container

AFB Urine Culture PTBU 20 – 50 mL urine Three (3) first morning, clean catch midstream samples only. BCCDC Lab Urine Mycobacteriology via SPH Microbiology Lab

Alanine Amino-Transferase ALT 1 PST Light green 0.5 mL lithium heparin plasma, send on ice pack 24 Hour SPH Chem. (SGPT) top

Albumin ALB 1 PST Light green 0.5 mL lithium heparin plasma, send on ice pack 24 Hour SPH Chem. top

Albumin - CSF ALBC 1.0 mL CSF Refrigerate CSF. Weekly VH Chem

Division of Pre and Post Examination, Page 7 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 0.5 mL fluid. Synovial fluid Albumin not available. Albumin – Fluid FALB PST, SST, RED 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF top or non FTYPE preservative PPD Dialysate FTYPE codes (get PET info from Modifier field): container DIAL24 = Dialysate 24hr DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3 Albumin Excretion Rate See albumin-urinary-micro

Alcohol ALC 1 PST Light green 0.5 mL lithium heparin plasma, send on ice pack. DO NOT USE 24 Hour SPH Chem (Ethanol, Ethyl Alcohol) top ALCOHOL SWAB TO CLEAN. Samples for suspected impaired drivers should be collected by the emergency physician and not by a laboratory staff.

Alcohol (Urine) NCODE Random urine Random urine. No preservative. If cannot send same day, LifeLabs within 3 days of refrigerate. Put Fax Result to SPH Lab request sticker on collection requisition or on Sunset Printout.

Aldolase Unavailable

Aldosterone (AV SITES) AVSAC If the order is for Aldersterone only RED top serum is acceptable. Weekly SPH Special Chemistry 3 mL EDTA on ice If the order is for Aldosterone and Renin, collect in pre chilled EDTA tube and then place on ice, centrifuge at 4°C ASAP (within (RED top serum is 15 minutes). 1.0 mL EDTA plasma store and send Frozen (dry acceptable for ice preferred). Aldosterone samples) Copy of requisition for Special Chemistry. The top table of req is PRE sampling, bottom table is POST sampling. Order Entry: free text AVS sample number and AVS site in Modifier field and result AVNUM, AVSITE (second screen): e.g. AVNUM = ;2f enter HIDE if no tube # given on requisition AVSITE = ;Pre Left Adrenal ***PRE and POST must be at beginning of AVSITE free text.

Division of Pre and Post Examination, Page 8 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Aldosterone Post Saline SALARB 3 mL EDTA on ice If the order is for Aldersterone only RED top serum is acceptable. Weekly SPH Special Chem Lab Suppression If the order is for Aldosterone and Renin, collect in pre chilled (RED top serum is EDTA tube and then place on ice, centrifuge at 4°C ASAP (within acceptable for 15 minutes). 1.0 mL EDTA plasma store and send Frozen (dry ice Aldosterone preferred). Specimen taken 4 hours after saline infusion. samples) Copy of requisition for Special Chemistry (esp. if there is 4 hours between collection times and no indication of saline suppression request).

Aldosterone (Random) ALDRB 3 mL EDTA on ice If the order is for Aldersterone only RED top serum is acceptable. Weekly SPH Special Chem Lab If the order is for Aldosterone and Renin ollect in pre chilled EDTA (RED top serum is tube and then place on ice, centrifuge at 4°C ASAP (within 15 acceptable for minutes). 1.0 mL EDTA plasma store and send Frozen (dry ice Aldosterone preferred). samples)

Aldosterone (Supine) ALDSUB 3 mL EDTA on ice If the order is for Aldersterone only RED top serum is acceptable. Weekly SPH Special Chem Lab If the order is for Aldosterone and Renin, collect on pre chilled (RED top serum is EDTA tube and then placed on ice, centrifuge at 4°C ASAP (within acceptable for 15 minutes). 1.0 mL EDTA plasma store and send Frozen (dry ice Aldosterone preferred). samples) Collect blood after one hour in the prone position.

Aldosterone (Upright) ALDUPB 3 mL EDTA on ice If the order is for Aldersterone only RED top serum is acceptable. Weekly SPH Special Chem Lab If the order is for Aldosterone and Renin, collect on pre chilled (RED top serum is EDTA tube and then placed on ice, centrifuge at 4°C ASAP (within acceptable for 15 minutes). 1.0 mL EDTA plasma store and send Frozen (dry ice Aldosterone preferred). samples) Collect after the patient has been awake ambulating and/or seated in upright posture. The patient should not have been lying down at any time two hours prior to collection.

Division of Pre and Post Examination, Page 9 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Aldosterone (Urine) ALDUU 24 Hr Urine, no 3 mL urine. Keep frozen. Patient should be off β-blockers and Twice SPH Special Chem Lab preservative diuretics for 2 weeks prior to test. Refrigerate during collection. monthly Add CRU, Measure 24 hour volume. Aliquot 3 mL in 12 x 75 plastic tube for UTIM (Unsuitable if urine creatinine, 3 mL in 12 x 75 plastic tube for urine Aldosterone collected in Acid) from well mixed urine (do not send whole collection). Centrifuge and decant both aliquots. Freeze Aldosterone tube, refrigerate creatinine and send on both on ice pack. Referring sites need to include 24 hour volume, patient’s height and weight on requisition.

Alkaline Phosphatase ALKP 1 PST light green 0.5 mL lithium heparin plasma, send on ice pack. 24 Hour SPH Chem top

Alkaline Phosphatase – Fluid FALKP PST, SST, RED 0.5 mL fluid. Synovial fluid ALKP not available. 24 hour` SPH Chem top or non FTYPE FTYPE: PLEUR; DIA; PERIT; BAL; PCF preservative container PPD Dialysate FTYPE codes (get PET info from Modifier field): DIAL24 = Dialysate 24hr DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3 2 mL plasma Alkaline Phosphatase ALKIA 1 PST light green Every 2 Y VGH Lab Isoenzymes top Patient should avoid high fat meal. weeks (Alkaline Phosphatase (Serum VGH will only perform if alkaline phosphatase level is elevated. Fractionated) acceptable) Freeze plasma or Serum.

Allopurinol And Oxypurinol NCODE 5 mL serum, separate and freeze Y Sunnybrook Medical Centre 2 RED top Attn: Dr. Adel Fam Send on dry ice. Approval required for all patients. Room A2002 Copy of requisition for send out. Put Fax Result to SPH Lab 2075 Bayview Ave. request sticker on requisition or on Sunset Printout. Toronto, Ontario M4N3M5

Alpha 1 Antitrypsin AAT 1 SST Gold top 0.5 mL serum send on ice pack Tues/Fri SPH Special Chem

Division of Pre and Post Examination, Page 10 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) University of Alberta Hospital Alpha 1 Antitrypsin Fecal AATS Fresh Random Prior consultation required. Minimum 5g stool in sterile container. Y Laboratory Medicine and Pathology (Suspect Protein Losing Stool (Walnut Freeze as soon as possible (within 30 minutes) after collection. Walter Mackenzie Centre Enteropathy) size) Outpatients: instruct patient to keep sample cool and transport to Specimen Control the lab as soon as possible. Core Lab Room 4B210 Freeze and send on dry ice. 8440-112 Street Edmonton, AB T6G 2B7

Alpha 1 Antitrypsin Phenotyping AATGPP 1 RED top and Test package AATGPP=AATPB and A1ATGB requires approval. SPH Special Chemistry (Phenotypes and Genotype 1 SST Gold top Please give a copy of requisition to Special Chemistry. package) and 1 LAV top Referring Labs: Aliquot serum from RED top and SST tubes. Do not open or aliquot LAV top. Freeze and send on dry ice: Monthly • 1 mL red top serum for Phenotype • 1 mL serum for AAT (Gold or Red top) • 1 LAV unopened tube for A1ATGB (Genotype) Send on dry ice to SPH Lab (MSJ Lab: aliquot red serum and freeze and send with LAV and GOLD with req. to SPH for approval.) SPH Accessioning: give ALL specimens to Special Chem. Children’s Hospital SPH Lab Sendout: send AATPB to CW on dry ice when instructed by Special Chem. Hospitals In-Common Laboratory Alpha 2 Macroglobulin* NCODE 1 SST Gold top 1 mL serum. Send frozen on dry ice. Approval required for all 16 days Y Inc. patients. *Unavailable For 57 Gervais Dr. Additional information on HICL website: Immunodeficiency Clinic At North York, ON M3C 1Z2 http://www.HICL.on.ca/search_tcna.asp?tcstring=a2mg SPH. Phone 416-391-1499 Ext.248 Fax 416-385-1957 Alpha Amino Nitrogen 20 mL aliquot/24 No preservative. Freeze. Keep specimen cool during collection. Y Children’s Hospital NOT AVAILABLE. 11/2014 HR urine Copy of requisition for send out.

Division of Pre and Post Examination, Page 11 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

AASA, Urine AASA Random urine in Concentrated morning specimen preferred. Optimal 5 mL, 8-12 Y Children’s Hospital sterile container minimum 2 mL in non preservative sterile container. weeks Alpha aminoadipic semialdehyde, Pyridoxine Copy of requisition for approval. Freeze urine and send frozen. dependent epilepsy, PDE, Antiquitin deficiency, B6 dependent epilepsy, Pyridoxine dependent seizures, P6C, Piperideine 6 carboxylate

Alpha Fetoprotein – BCCA ALFPB 1 SST Gold top 2.0 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control For Fluids: order FLDTYP in Sunquest, C/O PHSA Laboratories – Lane Fluid SPECFL =;Fluid type e.g. Peritoneal Fluid Level Laboratory

Alpha-Fucosidase AFUC 1 DKGRN top Prior consultation is required. Do not spin. Keep at room Y Children’s Hospital – Biochemical Lithium heparin temperature. Same-day shipping to have specimen arrive by Genetics Lab

1200 hrs, is preferred. 604-875-2307 *whole blood* If shipping overnight, use overnight courier, to have specimen arrive by 1200 hrs, the day after collection. Inform Biochemical Genetics Lab to expect sample. Whole blood, ship at room temperature to Specimen Receiving Room 2J20. Send copy of requisition with transport batch.

Alpha-Galactosidase, WBC AGAL 1 DK GRN top Minimum 3 mL whole blood. Keep at room temperature. Collect Y Children’s Hospital – Biochemical Lithium or Na and hand deliver to Sendout person before 08:30 Monday – Genetics Lab (Fabry Disease) heparin Friday or consult Supervisor before collection. Copy of requisition Room 2F22 for Sendout. *whole blood* 604-875-2307 SPH Sendout person: Send specimen to Children’s without approval with 09:30 Dynamex Courier. Then give copy of requisition for approval with “Specimen already sent to CW” written on requisition. Specimens collected after 09:30: Seek approval before sending. Same day shipping by taxi, to arrive at Children’s Hospital Lab by 12:00 hrs, (no later than 13:00 Mon-Fri, DO NOT use CW Courier).

1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Division of Pre and Post Examination, Page 12 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Alpha-Glucosidase, NCODE 2 DARK GREEN Prior consultation is required. Do not spin. Keep at room Y Children’s Hospital – Biochemical Lymphocytes top tube whole temp. Must deliver to BCCH within 3 hours of collection so blood Genetics Lab blood arrives prior to 1400 on Mon to Fri. Copy of requisition for send Room 2F22 Order AGLUB instead. out. 604-875-2307

Alpha-Glucosidase, Blood Spot AGLUB blood DOT CARD Optimal: 4 spots, minimum 2 spots. Follow collection instructions Children’s Hospital – Biochemical on reverse of blood dot card. Genetics Lab (POMPE testing), Acid Whatman 903 604-875-2307 maltase, bloodspot; Blood Spot Card Preferred: Collect using syringe and drip blood on blood spot glycogen storage II, card. bloodspot; a-glucosidase, Acceptable: bloodspot Collect 1 – 2 mL Heparin blood and transfer to blood spot card. Heparin blood must be transferred to blood spot card within 6 hrs of collection. If using finger/heel prick, allow blood to drip rather than touching blood spot card with patient’s finger/heel.

Obtain minimum one completely filled circle that is soaked through the back of card.

Write Pompe clearly on blood dot card. Do NOT use the pneumatic tube system to transport wet bloodspot cards. Wet bloodspot cards must NOT be packaged in biohazard bags. Allow to blood spots to dry at least 4 hours. Once dry, place blood spot card in sealed plastic bag with a sachet of desiccant (if available). Store in 4°C if there is delay in shipping. Ship at room temperature by overnight courier to Specimen Receiving 2J20. Inform lab at 6048752307 to expect the sample.

Send copy of requisition with transport batch.

Division of Pre and Post Examination, Page 13 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Alpha-Iduronidase, WBC AIDU 1 DK GRN top Prior consultation and approval by Children’s Lab Biochemical Y Children’s Hospital – Biochemical Lithium heparin Geneticist is required. Genetics Lab Room 2F22 *whole blood* Minimum 3 mL whole blood. Keep at room temperature. Copy of requisition for Sendout. Collect Monday – Thursday only or 604-875-2307 consult Supervisor. SPH Sendout person: Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Alpha-Mannosidase WAMAN 1 DK GRN top Prior consultation and approval by Children’s Lab Biochemical Y Children’s Hospital – Biochemical Lithium heparin Geneticist is required. Genetics Lab Room 2F22 *whole blood* Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room temperature. Copy of requisition for Sendout. Collect Monday – 604-875-2307 Thursday only or consult Supervisor. SPH Sendout person: Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Division of Pre and Post Examination, Page 14 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Alpha-Subunit Pituitary Tumor NCODE 1 RED Top Minimum 0.35 mL RED top serum, freeze. Send frozen on dry Y Specimen Process Centre Marker, Serum ice. Stability: 7 days refrigerated, 90 days frozen. Mayo Medical Laboratories 3050 Superior Drive NW Mayo Test ID: APGH Ensure Bill 73 is completed and copy of requisition for Sendout Rochester, MN 55901 bench. Alpha Glycoprotein Subunit 1-800-533-1710 SPH Sendout: must generate Mayo Clinic Order in MayoLink, Alpha Subunit, HCG see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= Alpha-HCG (Human NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG Chorionic Gonadotropin) QTgyREYy%0A

Mandatory MSP pre-approval required for Outpatients Alpha-PGH (Pituitary (request to be done by patient Dr.). For Outpatients, do not Glycoprotein Hormone) collect without MSP preapproval if test is ordered alone.

Alpha-Subunit of PGH (Alpha-PGH),

Serum Chorionic Gonadotropins, Alpha- Subunit

Glycoprotein Subunit

HCG, Alpha Subunit

Hormone, Alpha-Subunit

PGH (Pituitary Glycoprotein Hormone)

Pituitary Glycoprotein Alpha Subunit

Pituitary Gonadotropins, Alpha-Subunit

Division of Pre and Post Examination, Page 15 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Alpha Thalassemia MDT 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of N - if ordered Children’s Hospital requisition for send out. by Drs.: (Do not >2 mL Urine Ezzat, use Foltz ATHCW S. Jackson, code) C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi H. Merkley & P. Yenson

Alprazolam (Xanax) ALPRB 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. >2 mL Urine

Aluminum AL 1K2EDTA Dark 1.0 mL K2EDTA plasma, freeze Simport® Polystyrene aliquot N – Renal VGH Lab MSJ Outpatients – send blue top tube upright. Send on ice pack. Copy of requisition for send out. patients only patient to VH or SPH for Special Collection procedure link path (copy and paste to your *Special Collection Y – all others special collection. Intranet browser): tube from \\vch\departments\Chemistry (Dept Supervisor* PHCLAB)\Accessioning\Collection Booklet\Aluminum Collection and Processing Procedure.doc

Amantadine (Symmetrel) AMANB 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. >2 mL Urine

Amikacin (Pre/Trough) AMIPR 1 PST Light green 1.0 mL plasma. Freeze plasma. Provide dose time, must be ½ hr VGH Lab top Pre, and 1 hr Post dose. Sunquest Last Dose format: * SST serum also AMIDLD: ;DD Mmm YYYY e.g. 31 Mar 2014 or UNAVOE acceptable* AMITLD: ;HH:MM e.g. 13:50 or UNAVOE

Amikacin (Post/Peak) APO 1 PST Light green 1.0 mL plasma. Freeze plasma. Provide dose time, must be ½ hr VGH Lab top Pre, and 1 hr Post dose. Sunquest Infusion format: * SST serum also ITSA: ;HH:MM acceptable* ITFA: ;HH:MM

Division of Pre and Post Examination, Page 16 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amikacin Random Level AR 1 PST Light green 1.0 mL plasma. Freeze plasma. Provide dose time, must be ½ hr VGH Lab top Pre, and 1 hr Post dose. * SST serum also Sunquest Last Dose format: acceptable* AMIDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE AMITLD: ;HH:MM e.g. ;13:50 or UNAVOE

Amilodipine AMILOB 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. >2 mL Urine

Amino Acids (Urine) – Random RUAM Random sample, On approval of children’s Medical Biochemist. Must mix urine Y Children’s Hospital early morning with magnetic stir bar for 5 minutes before removing aliquot for Add CRR collection. creatinine. Ship entire remaining specimen frozen, do not split with other testing. Send copy of requisition with transport batch.

Timed Urine Amino Acids TUAM 24 hr urine Note: Must mix urine with magnetic stir bar for 5 minutes Children’s Hospital add CRU, before removing aliquot for creatinine. Ship entire remaining UTIM specimen frozen, do not split with other testing. Send copy of requisition with transport batch.

Amino Acids Urine Normalized UAMC Random Urine Entire urine collection required. Freeze entire sample and send Y Children’s Hospital frozen. (cystinuria monitor and screen, Amino Aciduria, Hartnup disease, Argininosuccinic aciduria, Renal tubulopathy, HHH syndrome, LPI)

Amino Acids Urine Timed UAMT Random Urine Specific order for KNOWN cystinuria patients only. Entire urine Y Children’s Hospital Output collection required. Freeze entire sample and send frozen.

Amino Acids Urine Absolute UAMA 24 Hour Urine If 24 hour urine cystine is requested, check if patient is a known Y Children’s Hospital container, no Cystinuria by calling BCCH Biochemical GeneticsLab at (604) (Amino Acids, 24 hour urine) additive 875-2307 or patiet’s doctor. 24 hour urine cystine is ONLY done on KNOWN Cystinuria patients, If ordered specifically by doctor for monitoring purposes. **Entire 24 hour urine collection required. Freeze entire 24 hour urine container and send frozen.**

Division of Pre and Post Examination, Page 17 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amino Acids, Bloodspot BAM Heel or finger prick Optimal 4 spots, minimum 2 spots. Specimen must be on Children’s Hospital on Whatman 903 supplied blotter card (New born screen card). Place barcode on Bloodspot amino acids, For Newborn Screening Laboratory NBS card back of NBS card after collection. Bloodspot phenylalanine, confirmed 4480 Oak Street, Room 2F27 (Newborn Bloodspot PKU, PKU monitor, PKU Allow blood dot cards to dry flat 2-3 hours first. Vancouver, BC V6H 3V4 Screening card, Amino acid monitor, deficient Do not insert wet samples inside glassine envelopes. Avoid high Blood Dot Card) Citrullinemia Monitor, MSUD patients temperatures. Monitor, Tyrosinemia Monitor, only. Do Do NOT use the pneumatic tube system to transport wet Bloodspot succinylacetone, not use bloodspot cards. Wet bloodspot cards must NOT be packaged Bloodspot citrulline, Bloodspot this code in biohazard bags. Leucine. Phenylalanine, for initial Phenylalanine Monitor; Blood Newborn Dot Card Phenylalanine screening Scan on transport batch and courier each sample as soon as it is dry. Ship at room temperature.

Amino Acids Chromatography PAMINO Process STAT Y Children’s Hospital – Biochemical Newborns: 1 DK Genetics Lab (Test Includes: Alanine, A- Adult must be fasting 12 hours or overnight, Babies must fast a GRN top Lithium Room 2F22 Amino-N-Butyric Acid, minimum of 4 hours. heparin on ice Arginine, Asparagine, 604-875-2307 Do NOT use Ammonium Heparin Aspartic Acid, Citrulline, *Na Heparin Cystine, Glutamic Acid, acceptable* Contact Medical Biochemist for approval Glutamine, Glycine, Adult: 2 DK GRN Collect on ice and centrifuge at 4°C within 60 minutes of Histidine, Hydroxyproline, top Lithium heparin collection. Do NOT use Buffy layer, remove plasma and freeze. Isoleucine, Leucine, Lysine, on ice Send on dry ice within 3 days of collection. Methionine, Ornithine, Phenylalanine, Proline, *Na Heparin Send copy of requisition with transport batch. acceptable* Serine, Taurine, Threonine, Tyrosine, Urea, Valine) (If from WGH, forward sample to BCCH if already frozen)

Division of Pre and Post Examination, Page 18 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amino Acid Quantitation, CSF CAMINO 1 mL CSF keep Optimal 1 mL CSF, minimum 0.5 mL. Y Children’s Hospital – Biochemical cold Genetics Lab Alanine, a-amino-N-butyric Blood stained CSF can give falsely high amino acid values. If Room 2F20 acid, arginine, asparagine, and CSF sample is blood stained, spin at 1800 g, take off CSF aspartic acid, citrulline, cystine, supernatant and freeze. Note on requisition that CSF was blood 1 SST Gold top glutamic acid, glutamine, stained and CSF was taken off after spinning. glycine, histidine, hydroxyproline, isoleucine, Send frozen CSF on dry ice as soon as possible. leucine, lysine, methionine, ornithine, phenylalanine, Also send blood sample. proline, serine, taurine, Send copy of requisition with transport batch. threonine, tyrosine, urea, valine.

Amino Levulinic Acid (Urine) ALAU 50 mL urine 24 hr collection. No preservative. Protect from light pH 4.0-7.0. Y VGH Lab (Delta-Aminolevulinic Acid) Refrigerate. Freeze if > 2 weeks old. Copy of requisition for send out.

Aminosalicylic acid SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; Aminosalicylic acid SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Amiodarone & Metabolite AMDRB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Centre dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Division of Pre and Post Examination, Page 19 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

>2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Aminodarone Amitriptyline & Metabolites AMIB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre (Elevail) by HPLC requisition for send out. Ward should record last dose time. Sample should be collected as a trough sample. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Amitriptyline Amlodipine SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; AMLODIPINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Amobarbital (Amytal) AMOBB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 20 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Ammonia NH4 1 PST Light green 2 mL minium plasma for adults. 0.3 mL minimum plasma for N Children’s Hospital top on ice babies. Do NOT use a tourniquet during collection. Venous blood

must be collected on ice and deliver to the Lab IMMEDIATELY. For Babies: 1 full Centrifuge @ 4°C. Separate plasma within 15 minutes. draw PST Light Freeze immediately. Test within 24- 48 HRS of collection. green top Send frozen on dry ice. microtube

*Sodium heparin acceptable* Must be venous or line collection only. Amoxapine (Asendin) AMOXB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 21 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amoxicillin SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Ward should supply last dose time. Specimen should be a trough

sample. Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; AMOXICILLIN SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Amphetamine AMPTB 1 RED top 2-4 mL serum orefrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre (Speed Quantitative) requisition for send out.

Amphetamines In Urine AMPHP Random urine >1.0 mL urine. Refrigerate if not done immediately. Order UDS 24 Hour Y – if DRSCB SPH Chem (Outpatients) or UDP if screen ordered. Add DRSCB if Dr ordered Ecstasy Part of specifies Amphetamines confirmation (or aka names) and give MDA UDS, or copy of requisition for Medical Biochemist approval. (Methylenedioxyamphetami UDP ne) MDMA (Methylenedioxymethamphe tamine) Methamphetamines (Desoxyn) Speed (Amphetamines)

Division of Pre and Post Examination, Page 22 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amphetamines In Urine, DRSCB Urine Refrigerate. Send 50 mL Urine on ice pack. Y Provincial Toxicology Centre Confirmation Sunquest LIS Order Entry:

*If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Amphetamines confirmation

Amphotericin B SPQ 6 mL LAV top 3 mL EDTA Plasma. Collect trough level prior to dose level and ProvLab-Alberta (Edmonton Site) Collect peak level (2 hour Post dose) or IV peak level (30 minutes Contact: Dr. Jeff Fuller, Clinical Post dose). Freeze. Send on ice pack. For further info call 604- Scientist, Mycology Section, 806-68184. Send creatinine result with specimen. 780-407-7242 ***protect from light*** OR: The Fungus Testing Laboratory BCCDC Toxicology is developing this test. Call Prov Tox before Department Of Pathology, Room sending test out of Province (require Med Micro approval). Feb 329E. Mail Code 7750 28, 2013. The University Of Texas Health Science Center at San Antonio Sunquest Order Entry instructions: free Text in the following fields San Antonio, Texas 78229-3900 SD0177 = PLS-EDTA Phone: (210) 567-4131 SPQ1 = ;Amphotericin B SQDLD = ;DD MMM YYYY e.g. ;15 APR 2013 or UNAVOE SQTLD = ;HH:MM e.g. ;07:00 or UNAVOE Amylase AMY 1 PST Light green 0.5 mL lithium heparin plasma, send on ice pack 24 Hour SPH Chem. top 0.5 mL fluid. Synovial fluid Amylase not available. Amylase, Fluid FAMY PST, SST, RED 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF top or non

Division of Pre and Post Examination, Page 23 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Amylase Creatine Ratio, Urine AMCR Urine and 5 mL aliquot of urine and 1 mL plasma or serum. 24 hour VGH Lab 1 PST Light green Specimens should be collected at the same time. top or SST Gold Refridgerate, send on ice pack. top

Amylase, Urine , Random AMYR Urine 5 mL aliquot of urine. 24 Hour VGH Lab Refridgerate, send on ice pack.

Amylase, Urine, 24 Hour AMYU Urine, 24 hour Send a 5 mL aliquot of urine to VGH for amylase testing. 24 Hour VGH Lab collection CRU Refridgerate, send on ice pack. UTIM

Amylase Isoenzymes AMISO 1 mL serum Refrigerate Unavailable 05/29/00 Y Vancouver Hosp –Lab Recommend Serum lipase, if special consideration, amylase creatinine clearance is method of choice. (VGH). Revised Aug/03.

Anaplasma Phagocytophila EHRL 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control Serology (BCCDC Sendout)

Androstenedione ANDRO 1 RED top 1 mL red top serum. Freeze specimen and send on ice pack. Children’s Hospital

Angiotensin Converting ACEB 1 SST Gold top Send 2 mL serum preferred minimum 0.5 mL. Store and send on Mon - Fri Victoria General Hospital, 1 Hospital Enzyme, Serum (ACE) ice pack. Copy of requisition for send out. Put Fax Result to Way, Victoria, BC, V8Z 6R5 1-866- SPH Lab request sticker on requisition or on Sunset Printout. 370-8355

Angiotensin Converting CACEB 1 mL CSF Store and send on ice pack. Copy of requisition for send out. Put Mon – Victoria General Hospital, 1 Hospital Enzyme, CSF Fax Result to SPH Lab request sticker on requisition or on Fri Way, Victoria, BC, V8Z 6R5 1-866- Sunset Printout. 370-8355

Anileridine (Lertine) ANILB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Anthrax Serology ATRX 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Anti-A Titre ABOAT Two 6 mL LAV top Minimum 2 full Tall EDTA top tubes. 2-4 SPH Transfusion Medicine (Blood (Anti A titre; Antibody A titre) Hours Bank) Mon-Fri,

Anti 21 Hydroxylase Ab NCODE 1 RED top 1.0 mL serum. Freeze and send on dry ice. Stability 14 days Y Specimen Process Centre 21-OH Ab frozen. Ensure Bill 73 is completed and copy of requisition for Mayo Medical Laboratories Hydroxylase Antibody Sendout bench. 3050 Superior Drive NW OH21 Ab SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 21OH Ab see Supervisor: 1-800-533-1710 Mayo Test ID: OH21 https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Anti Adrenal Antibody NCODE 1 RED top 2.0 mL serum. Freeze and send on dry ice. Copy of requisition Y Royal Jubilee Hospital for send out. Put Fax Result to SPH Lab request sticker on (CW code requisition or on Sunset Printout. ADREAB blocked from SPH)

Anti AMPA antibodies NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582 Anti Basal Cells Unavailable

Anti-Centromere Antibody ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab days @ 4°C. Login Order comment field & Modifier field: Anticentromere antibody ordered

Anti Cyclic Citrullinated Peptide CCPEP 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab Antibody (Anti CCP)

Division of Pre and Post Examination, Page 25 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Anti D NCODE Two 6 mL LAV top Complete CBS Antibody Investigation Request Form and write CBS via SPH TM and “Check for Anti-D on form” Blank request forms in Forms/Reqs 1 RED top section. Copy of requisition for send out. Hospitals In-Common Laboratory Anti Diuretic Hormone ADHB 35 days Y Two 6 mL LAV top Patient must fast, and no water allowed. Spin cold. Aliquot 3 mL Inc. (ADH) on ice plasma into 2 tubes and write “EDTA plasma”. Freeze both tubes 57 Gervais Dr. (Arginine Vasopressin) at –20°C. ***Send frozen sample on dry ice*** North York, ON M3C 1Z2

Additional information on HICL website: http://www.HICL.on.ca/search_tcna.asp?tcstring=adh Phone 416-391-1499 Ext.248 Copy of requisition for send out. Put Fax Result to SPH Lab Fax Phone 416-385-1957 request sticker on requisition or on Sunset Printout. Anti-DNA only DSDNAS 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. VGH Lab (ADNA, SLE, double stranded Use this code DNA) if no other auto-immune tests ordered

Anti-DNA with other ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab autoimmune tests ordered days @ 4°C.

Anti-GABAb NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler send frozen on dry ice. Mitogen Advanced Diagnostics Lab 1 SST Gold top HMRB 431 – 3330 Hospital Dr. NW Use Mitogen Diagnostics Laboratory Requisition (in the Calgary, AB, T2N 4N1 Requisitions Folder of the Accessioning Folder). Phone 403-220-4582 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Anti Ganglioside GM-1 GM1A 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Antibody send frozen on dry ice. Mitogen Advanced Diagnostics Lab 1 SST Gold top (Ganglioside GM-1 Antibody, HMRB 431 – 3330 Hospital Dr. NW Use Mitogen Diagnostics Laboratory Requisition (in the Peripheral Neuropathy: Calgary, AB, T2N 4N1 Requisitions Folder of the Accessioning Folder). Autoimmune) Phone 403-220-4582 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout..

Division of Pre and Post Examination, Page 26 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Anti Ganglioside CO-GM1 NCODE 1 RED top 2.0 mL RED top serum. Separate Serum and freeze. Send Y Athena Diagnostics Laboratory Antibody Serum on dry ice Four Biotech Park, Use Athena Diagnostic requisition. 377 Plantation Street, Peripheral Neuropathy: Test panel #270 Worcester, MA 01605 Autoimmune Copy of requisition for send out. Put Fax Result to SPH Lab (800) 394-4493 request sticker on requisition or on Sunset Printout. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Anti Glycolipid Antibody NCODE 1 RED top tube Serum, frozen on dry ice. Y Preston Thompson, Room 507, College Building, 1025 Walnut St., Information out of date Feb/06. Thomas Jefferson University, Philadelphia, Penn. 19107

Anti Histone Antibody ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab days @ 4°C.

Anti HIV Ag/Ab Combo HIVCC 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control blocked from SPH Test code is blocked at PHC, use order code HIVCA. ordering

Anti IgA AIGA 1 RED top or Minimum 2 mL RED top serum OR 2 mL EDTA plasma, freeze CBS via SPH TM and send on ice pack. 6 mL LAV top Fax “patient request for IgA/Anti-IgA testing” requisition (CBS Req.) To ordering Dr office. Send completed requisition with sample Hospitals In-Common Laboratory Anti-Intrinsic Factor Antibodies NCODE 5mL SST Gold top 3 mL serum. Freeze. Send frozen on dry ice. 30 days Y Inc. Additional information on HICL website: 57 Gervais Dr. http://www.HICL.on.ca/search_tcna.asp?tcstring=infab North York, ON M3C 1Z2

Copy of requisition for send out. Put Fax Result to SPH Lab Phone 416-391-1499 Ext.248 request sticker on requisition or on Sunset Printout. Fax Phone 416-385-1957 Anti-Mitochondrial Antibody AMA 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab Anti-Mitotic Spindle ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab (Enter As ANAEB) days @ 4°C.

Division of Pre and Post Examination, Page 27 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Anti-Mullerian Hormone MULAB 1 RED top 2 mL serum from RED top. Separate and freeze ASAP. Send 20 days Y Inc. frozen on dry ice. Approval required for all patients. Put Fax *1 SST Gold top, 57 Gervais Dr. Result to SPH Lab request sticker on requisition or on Sunset LAV top, PST Light North York, ON M3C 1Z2 Printout. green top acceptable* Additional information on HICL website: Phone 416-391-1499 Ext.248 http://www.HICL.on.ca/search_tcna.asp?tcstring=mulab Fax Phone 416-385-1957

Anti Nuclear Antibody ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab (ANA Pattern) days @ 4°C.

Anti Neutrophilic Cytoplasmic ANCA 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab Antibodies [C-ANCA(PR3), P- ANCA(MPO), Anti- Myeloperoxicase Ab, Proteinase 3, Perinuclear ANCA)

Anti Parietal Cell Antibody APCA 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab

Anti-Platelet Antibody NBTB Adult: 1 RED top Do not spin. Send on ice pack to CBS within 24h of collection. CBS via SPH TM and Three 6 mL Completed platelet antibody investigation request form must

LAV top accompany specimen. Blank forms found in forms/reqs section. Children under 12Y: 1 RED top and Two 6 mL LAV top

Anti-Reticulin Antibody ASMA 1 SST Gold top 1.0 mL serum. Freeze. Send frozen on ice pack VGH Lab

Division of Pre and Post Examination, Page 28 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Anti Retinal Antibody NCODE 2 RED top 5.0 mL Red top serum or EDTA plasma, minimum 3 mL. 6-8 Y Specimen Process Centre Stability: 24 hours Ambient, 14 days refrigerated, 30 days frozen. weeks Mayo Medical Laboratories Mayo Test ID: FARHC Frozen samples must be sent on dry ice and not allowed to thaw. 3050 Superior Drive NW Oregon Health requisition must be completed by ordering Dr. and Rochester, MN 55901 ensure Bill 73 is completed and copy of requisition for Sendout 1-800-533-1710 bench. Mayo Lab will forward to: SPH Sendout: must generate Mayo Clinic Order in MayoLink, Ocular Immunology Laboratory, see Supervisor: Oregon Health & Science University https://orders.mayomedicallaboratories.com/en/login?SAMLart= Casey Eye Institute – BRB, Room NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG 253 QTgyREYy%0A 3181 SW Sam Jackson Park Road Portland, OR 97239, USA Complete OHSU Ocular request form: 503-418-2543 http://www.ohsu.edu/xd/health/services/casey-eye/diagnostic- services/ocular-immunology-lab/upload/OILshippingreqform6- 12.pdf Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Anti-Ribosomal-P Antibody NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

Anti-Saccharomycee Cerviciae ASCA 1 SST Gold top 1.0 mL serum. Freeze. Send frozen on ice pack VGH Lab Antibodies (ASCA)

Anti-Smooth Muscle Antibody ASMA 1 SST Gold top 1.0 mL serum. Freeze. Send frozen on ice pack VGH Lab

Anti-Streptolysin-O Titre ASOTB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (ASOT, Anti DNase B, C/O PHSA Laboratories – Lane Antideoxyribonuclease, Level Laboratory Streptococcalenzyme – Slide Test, Streptozyme, Anti-Hyaluronidase)

Division of Pre and Post Examination, Page 29 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Full citrate draw. Specimen must be centrifuged within 2 hours Anti Thrombin III AT3 2 BLUE top citrate 1-2 Y SPH of collection. 1.5 mL double spun plasma per factor assay in 2 double spun times polypropylene aliquot tubes. Freeze at -20°C, refer in sample per week must arrive at SPH frozen. Copy of requisition to Special Coagulation. Carlsten Lab at Diamond Health Anti Thermophilic Actinomyces ATA 1 SST Gold top 0.5 mL serum. Refrigerate and send on ice pack to VGH . No Care Centre via VGH Lab need to aliquot if SST. Affix Lung Centre address label to the 7225 – 2775 Laurel Street. package for VGH to forward to Lung Centre. Please provide a th 7 Floor *1 RED top copy of original requisition. Put Fax Result to SPH Lab request Vancouver, BC, V5Z 1M9 acceptable (must sticker on requisition or on Sunset Printout. 604-875-4111 ext. 68989 or 66455 be aliquoted)* Do not freeze. Antithyroid Peroxidase Ab ATPOP 1 SST Gold top 0.5 mL SST serum in False Bottom or 13 x 75 Polypropylene Mon, SPH Special Chemistry tube. Freeze, send with ice pack up to 2 days after collection or Thurs, (Anti-microsomal antibodies) send frozen. This test has replaced the thyroid antibody titre. . Fri (Anti-TPO)

(Anti-thyroperoxidase antibodies) (Thyroid microsomal Ab) (ThyMic Ab)

Antibody-Investigation HLAMB Four 6 mL LAV Must include CBS antibody investigation request form obtained 2-6 hrs CBS via SPH TM top and from SPH Transfusion Medicine. Forward specimens to 8am – (HLA Antibody screen) Two 5 mL SST transfusion Medicine for shipping to CBS. 4pm Gold top Routine Antibody Investigation (PHC) PABI Two 6 mL LAV top SPH Transfusion Medicine (Blood & STAT: (only Bank) 2-4 orderable hours by TM) 24 hours

Division of Pre and Post Examination, Page 30 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Anti-Xa Activity – HEPQ 1 BLUE top citrate Full citrate draw. Specimen must be centrifuged within 2 hours Mon – SPH Special Coag (Factor Xa activity, αXa of collection. Fri Level, anti Xa level, Low Minimum 1.0 mL Plasma double spun, aliquot in Polypropylene Molecular Weight Heparin, tube. Freeze at -20°C, refer in sample must arrive at SPH Daltiparine, Danaparoid, frozen. Must know type of heparin and time of last dose – copy Enoxaparin, Fragmin, of requisition to Special Coagulation. Fraxiparine, Heparin Assay, Lovenox, Tinzaparin,Rivaroxaban, Fondaparinux)

HEPQ 1 BLUE top citrate Full citrate draw. Specimen must be centrifuged within 2 hours Mon – SPH Special Coag of collection. Fri

Minimum 1.0 mL Plasma double spun, aliquot in Polypropylene tube. Freeze at -20°C, refer in sample must arrive at SPH frozen. Must know type of heparin and time of last dose – copy of requisition to Special Coagulation. Apolipoprotein A APOA 1 SST Gold top 0.5 mL serum or lithium heparin plasma. Send on ice pack. Tues/Fri Y SPH Special Chem (APO A)

Apolipoprotein B APOB 1 SST Gold top 0.5 mL serum or lithium heparin plasma. Send on ice pack. Mon – Y SPH Special Chem (APO B) Fri *1 PST Light green Sunquest Order Entry last screen, Diagnosis required: APOBR if top acceptable* outpatient APOBDX= COMDYS: Complex Dyslipidemia HYPERT: Hypertriglyceridemia DYSHYP: Dysbetalipoproteinemia DXFU: Follow up of treated hypercholesterolemia DM: Diabetes Mellitus NGR: Information not on requisition or Free Text the Diagnosis

APO E (Genotype) APOEG 1 LAV top Do NOT spin or open EDTA tube. Store EDTA whole blood at Once SPH Special Chem (Apolipoprotein E Isoforms) 2 - 4°C. Ship with an ice pack within 7 days of blood collection. per month. Referring Labs: Prior consultation is required or provide lipid results.

APO E (Phenotype) Unavailable –Replaced BY APO E (GENOTYPE)

Division of Pre and Post Examination, Page 31 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

APTT PTTB 1 BLUE top citrate Full citrate tube collection required. 24 Hour SPH Coag (Activated Partial Plasma spun within 2 hours of collection and tested within 4 Thromboplastin Time, PTT) hours of collection. If test is not performed within 4 hours, double spin plasma and store at -20°C for up to 2 weeks, Ship on ice. Specimen must arrive frozen in polypropylene tube. ARBOVIRUS – This Code Has 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Been Discontinued C/O PHSA Laboratories – Lane

Level Laboratory

Arginase NCODE 1 DK GRN top Prior approval required. Cool and keep on ice, but do not allow Y Children’s Hospital – Biochemical Lithium Hep to freeze. Do not spin and ship immediately. Copy of requisition Genetics Lab for send out. Room 2F22 *whole blood* 604-875-2307

Arginino-Succinic Acid Lyase NCODE 2 DK GRN top Requires consultation. Please contact Children’s Hospital for Y Children’s Hospital – Biochemical Lithium Hep complete instructions. Copy of requisition for send out. Genetics Lab Room 2F22 *whole blood* 604-875-2307

Aripiprazole ARIPB 1 RED top 1.0 mL Red top serum. No Gold SST. Refrigerate and send on BCCDC Provincial Toxicology ice pack. Sunquest Order Entry instructions: Free Text in the following fields ARIDLD = ;DD MMM YYYY e.g. ;15 APR 2013 ARITLD = ;HH:MM e.g. ;07:00

Aripiprazole, Urine ARIPB Random urine Refrigerate and send on ice pack. BCCDC Provincial Toxicology Sunquest Order Entry instructions: Remove “R” container at Container and Specimen Entry. Select “UR” in Container field and then “Add” button. Free Text in the following fields ARIDLD = ;DD MMM YYYY e.g. ;15 APR 2013 ARITLD = ;HH:MM e.g. ;07:00

Arsenic (random urine) ARSR Random urine Use acid washed containers only. pH entire urine collection to Y VGH Lab between pH 2 to 4. (Includes Arsenic Creatinine Add CRR Ratio) Send 50 mL aliquot at ambient temperature.

Division of Pre and Post Examination, Page 32 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Arsenic (Urine) ASU Random OR 24 Use acid washed containers only. pH entire urine collection to Y VGH Lab HR. urine in 15 between pH 2 to 4. Add CRU, mL. 6mol/L HCL Send 50 mL aliquot at ambient temperature. UTIM acid cleaned bottle. Min. 50 mL.

Aryl Sulfatase A, WBC WASA 1 DK GRN top Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room Y Children’s Hospital – Biochemical Lithium Hep temperature. Copy of requisition for Sendout. Collect Monday – Genetics Lab Metachromatic leukodystrophy Thursday only or consult Supervisor. Room 2F22 *whole blood* SPH Sendout person: 604-875-2307 Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. Aryl Sulfatase B, WBC ASB 1 DK GRN top Prior consultation and approval by Children’s Lab Biochemical Y Children’s Hospital – Biochemical Galactosamine 4-sulphatase; Lithium Hep Geneticist is required. Genetics Lab N-acetylgalactosamine 4- Room 2F22 *whole blood* Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room sulphatase; ASB; Maroteaux- temperature. Copy of requisition for Sendout. Collect Monday – 604-875-2307 Lamy syndrome; MPS VI. Thursday only or consult Supervisor. SPH Sendout person: Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Division of Pre and Post Examination, Page 33 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Aryl Sulfatase C, WBC WASC 1 DK GRN top Testing is only offered for male patients. Minimum 3 mL whole Y Children’s Hospital – Biochemical add MBW Lithium Hep or Na blood. Keep at room temperature. Collect and hand deliver to Genetics Lab X-Linked ichthyosis; Steroid Hep Sendout person before 08:30 Monday – Friday or consult Room 2F22 sulfatase deficiency Supervisor before collection. Copy of requisition for Sendout. *whole blood* 604-875-2307 SPH Sendout person:. Send specimen to Children’s without approval with 09:30 Dynamex Courier. Then give copy of requisition for approval with “Specimen already sent to CW” written on requisition. Specimens collected after 09:30: Seek approval before sending. Same day shipping by taxi, to arrive at Children’s Hospital Lab by 12:00 hrs, (no later than 13:00 Mon-Fri, DO NOT use CW Courier). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. ASA SALI 1 PST Light green 0.5 mL Lithium heparin plasma. Send on ice pack 24 Hour SPH Chem (Acetylsalicylic Acid, top Salicylate, Aspirin) Ascaris Serology ASCAB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Atypical Hemolytic Uremic NCODE Two 6mL LAV top Collect only Mon – Wed, sample stability is only 48 hours; cost of Y Molecular Genetics Division Syndrome/ testing is $6000.00 for Panel A and B gene sequencing. Send at The Hospital For Sick Children Membranoproliferative room temperature. BEFORE collecting blood, patient must 555 University Avenue Glomerulonephritis provide: Room 3420, Hill Wing Panel A, Panel B Toronto, On, M5G 1X8 1. MSP pre-approval letter prior to collection. Tel: 416-813-7200 2. Patient’s doctor must have completed the SickKids Molecular Genetics Requisition link below: http://www.sickkids.ca/pdfs/Paediatric%20Laboratory% 20Medicine/2269-MolecGeneticsReq_BillingForm.pdf 3. Out of province genetic referral request: http://www.genebc.ca/uploads/CWMG_REQ_0210_v5_0_ Coordinating_Out_of_Province_Genetic_Testing.pdf

ASPERGILLUS SEROLOGY ASPS 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control (Order ASPPRB) Top tube

Division of Pre and Post Examination, Page 34 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Aspergillus-Serology Or ASPPRB 1 SST Gold top 0.5 mL serum. Refrigerate and send on ice pack to VGH . No Carlsten lab at Diamond Health Care Aspergillus-Precipitins need to aliquot if SST. Affix Lung Centre address label to the Centre via VGH Lab

package for VGH to forward to Lung Centre. Please provide a 604-875-4111 ext. 68989 or 66455 *1 RED top copy of original requisition. Put Fax Result to SPH Lab request acceptable (must sticker on requisition or on Sunset Printout. be aliquoted)* Do not freeze. Aspergillus Galactomannan CSGLAC 1 RED top Copy of requisition for Medical Microbiologist approval Tuesday Y See Supervisor – Antigen (Microbiology TRAP test). Aliquot serum and freeze at -70°C and TRAP By or Test available at C&W, Send to C&W Research freezer. Send on dry ice. Specimen must be received Friday Med. Micro. (Galactomannan Serum EIA) Virology if Instructed by Medical at C&W within 48 hours. Microbiologist. Bronchial Alveolar Medical Microbiologist to decide to send to C&W (after consult Lavage with C&W Med Micro) or BCCDC. AGAG If sample is to go to BCCDC, Order AGAG, modify BCCDC’s Specimen Description with Med Micro initials if approved: E.g. Galactomannan Assay SDES = SER, Modifier = AMMMR (Aspergillus antigen) Give copy of requisition to SPH Microbiology.

AST AST 1 PST Light green 0.5 mL lithium heparin plasma. Send on ice pack. 24 Hour SPH Chem. (SGOT, Aspartate top transaminase)

Atenolol ATNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 35 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Autohemolysis Unavailable

Autologous Parathyroid Tissue 2 RED top Allow clotting for 30 minutes. Centrifuge and deliver to Transfusion STAT SPH Transfusion Medicine (Blood Transplantation Medicine (Blood Bank) immediately. TM will remove Serum. For from Bank) (Cryopreservation –Serum) additional information, see form section for Autologous O.R. Parathyroid Tissue Transplantation. 1 hour

Mon – Fri

Autoimmune Liver Diseases NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Profile send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Test Includes: Soluble Liver Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Antigen (SLA), SP100, Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 gp210, PML, 3EBPO, LC-1, Put Fax Result to SPH Lab request sticker on requisition or on Ro52, Liver Kidney Sunset Printout. Phone 403-220-4582 Microsome (LKM), Mitochrondrial Oxaloacid Dehydrogenase M2/M3

Autoimmune Inflammatory NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, 7-10 Y Dr. Fritzler Myopathy/Myositis Profile send frozen on dry ice. days Mitogen Advanced Diagnostics 1 SST Gold top Laboratory (Anti Synthetase) Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Test Includes: Jo-1 (Histidyl Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 tRNA Synthytase, Mi2, PL7, Put Fax Result to SPH Lab request sticker on requisition or on Phone 403-220-4582 PL12, Pm/Scl75, Pm/Scl100, Sunset Printout. Ku, Ro52, SRP, EJ and OJ.

Division of Pre and Post Examination, Page 36 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Autoimmune Neurological GM1A 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Diseases Profile send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Tests Includes: Anti-GM1 (IgM, Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW IgG), GM2, GM3, GD1a, Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 GD1b, Gt1b, (IgM, IgG), Put Fax Result to SPH Lab request sticker on requisition or on GQ1b (IgM, IgG), (IgM, IgG) Sunset Printout. Phone 403-220-4582

Autoimmune Paraneoplastic HUYOB 1 RED top or 1 mL serum or CSFminimum. Prior approval is required. Freeze 5-7 days Y Dr. Fritzler Diseases Profile serum, send frozen on dry ice. (Wed. Or Mitogen Advanced Diagnostics 1 SST Gold top (Paraneoplastic Ataxia, Thurs.) Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the Encephalomyelitis) HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). 1 mL CSF: Calgary, AB, T2N 4N1 Tests Includes: Anti-Yo, Anti Put Fax Result to SPH Lab request sticker on requisition or on Hu, Anti Ri (NOVA-1), CSF orders Sunset Printout.CSF samples from SPH Hematology fridge can Phone 403-220-4582 Amphiphysin, should include 2 be used for these tests. –updated Dec/2013 CL. Amphyphysin, CV2/CRMP- mL serum from 5, PNMA2 (Ma2/Ta), SST Gold top Recoverin, SOX1, Titin.

Avian Precipitins (Budgie, IGGPS 1 SST Gold top 0.5 mL serum. Refrigerate and send on ice pack to VGH . No T Carlsten lab at Diamond Health Care Chicken, Pigeon, Etc.) need to aliquot if SST. Affix Lung Centre address label to the Centre via VGH Lab

package for VGH to forward to Lung Centre. Please provide a 604-875-4111 ext 66455 *1 RED top copy of original requisition. Put Fax Result to SPH Lab request acceptable (must sticker on requisition or on Sunset Printout. be aliquoted)* Do not freeze. Azatadine SPQ 1 RED top or 2-4 mL RED top serum. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=;AZATADINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Azathioprine Metabolites NCODE 6 mL LAV top 5.0 mL EDTA Whole Blood. Collect trough specimen 0-30 Y Inc. minutes prior to next dose. Store and send cold. Unsuitable if 57 Gervais Dr. specimen is frozen. Copy of Requisition for Sendout. North York, ON M3C 1Z2

Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

B Type Natriuretic Peptide BNP 1 LAV 1.0 mL plasma separated within 3 hours of collection into a 24 Hour SPH Chem Plastic tube, freeze. Plasma stable for 24 hours at 2 – 8°C. Test Discontinued. Special requisition required. Order LBNP

BNP NT LBNP 1 PST Light green 0.5 mL serum. Refrigerate, send on ice pack. Stable for 6 days at SPH Chemistry top 2-8 °C NT proBNP

N-terminal fragment of B type Inpatients: Do not repeat LBNP within 7 days unless approved *1 SST Gold top Natriuretic peptide by Biochemist or cardiologist. Do not repeat LBNP if value was acceptable* <100 within 30 days. MSJ- 1 SST Gold top

Babesia Serology BAB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Baclofen (Diban Or Lomotil) BACLOB 1 RED top or 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 38 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Basement Membrane GBM 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab Antibodies (Anti-Glomerular Basement Cell Membrane) BAME Quantitative Test not done – result as NRAVLH as of Jan/01. Add GBM if no previous positive GBM result.

Barmah Forest Virus Serology BFV 1 SST Gold top 2.0 mL serum. Refrigerate. Send on ice pack. BC Centre For Disease Control

Barbiturates BARBS 1 RED top 2-4 mL RED top serum. Consult Provincial Toxicology centre Y Provincial Toxicology Centre toxicology – 7627. State possible barbiturate. Lab will take blood Weekly Stat Requires Consent

at the time of the request

Barbiturates BARBS 20 mL gastric Send to lab in plain plastic container. Y Provincial Toxicology Centre specimen if

available as well.

Barbiturates screen, Urine BARB Random urine 1 mL random urine send refrigerated Y VGH Chemistry

Bartonella Henselae Serology BARTB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Bartonella Serology) C/O PHSA Laboratories – Lane Level Laboratory

Bartonella Species Nat NCODE 5 mL SST Gold top Do not use BSNAT order code. Refrigerate. Send on ice pack. Y – TRAP BC Centre For Disease Control (Bartonella PCR) Phone BCCDC ZEP lab if other specimen types. Modify REFTO1 with Med Micro initials if approved: E.g. REFTO1=;Sent to BCCDC-AMMMR

Division of Pre and Post Examination, Page 39 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 1 DRK GRN top CML CGBA Mon to Fri 0800 to 1500 HRS. Do not collect on Fridays without N - if ordered VGH Cytogenetics Lab Na hep approval. For Friday collections, alert VCH CG Lab 604-874- by Drs.: Diagnostic testing 4111 Local 62505. Do not collect on Saturday or Sunday or on Ezzat, FISH –BCR/ABL t(9:22) Statutory holidays. Foltz S. Jackson, Cytogenetics If unable to send the same day, leave specimen at room C.Leger, temperature and send to VCH Cytogenetics in the morning. H.Leitch, AML- M3/APL Copy of completed VGH Cytogenetics requisition or BCCA Ross(Boldt), Diagnostic testing requisition for Sendout person. Ramadan, H. FISH –PML/RARA t(15:17) Sun H. Cytogenetics Merkley & P. Yenson

CML (BCR/ABL ) – Minimal MGABB Refer to BCCA Mon to Thurs 0800 to 1500 HRS. Do not collect on Fridays N - if ordered B.C. Cancer Control Agency – Residual Disease (MRD) follow- requisition for Saturday or Sunday or on Statutory holidays, specimen stability by Drs.: Molecular Genetics Lab up (PCR), PCR For BCR-ABL specimen volumes: 48 hours. Ezzat, Must Call To Let Them Know It Is Translocation Four 6 mL LAV top Foltz If unable to send the same day, leave specimen at room Coming. S. Jackson, PML/RARA- AML- M3/APL temperature and send to BCCA in the morning. C.Leger, 877-6000, Loc. 2094 Chimerism for BMT 6 mL LAV top from Copy of BCCA Requisition for Sendout person. H.Leitch, both Donor and Ross(Boldt), Recipient Ramadan, H. Sun H. C-kit Mutation in MPD Two 6 mL LAV top Merkley & P. Yenson JAK 2 6 mL LAV top

Benzodiazepine DZPNB 1 RED top 2-4 mL RED top serum. Refrigerate Y Provincial Toxicology Centre (Diazepam, Valium, Desmethyldiazepam, Tranxene)

Division of Pre and Post Examination, Page 40 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Benzodiazepine In Urine BENZ Random urine >1.0 mL urine. Refrigerate if not done immediately. Order UDS 24 Hour Y – if DRSCB SPH (Outpatients), or UDP if screen ordered. Add DRSCB if Dr ordered Ativan (Lorazepam) Part of specifies the Benzodiazepine confirmation (or aka names) and Benzodiazepines UDS, give copy of requisition for Medical Biochemist approval. Chlordiazepoxide (Librium) UDP Dalmane (Flurazepam) panel Diazepam (Valium) Flunitrazepam (Rohypnol) Flurazepam (Dalmane) Halcion (Triazolam) Librium (Chlordiazepoxide) Lorazepam (Ativan) Oxazepam (Serax) Restoril (Temazepam) Rohypnol (Flunitrazepam) Serax (Oxazepam) Temazepam (Restoril) Triazolam (Halcion) Valium (Diazepam) Xanax (Alprazolam) 7-Amino Flunitrazepam (Metabolite of Flunitrazepam) (Rohypnol) Alprazolam (Xanax) Hydroxy-Ethyl Flurazepam (Metabolite of Flurazepam)

Benzodiazepine In Urine, DRSCB Urine Refrigerate if not done immediately. Send 50 mL of urine on ice Y Provincial Toxicology Centre Confirmation pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Benzodiazone confirmation

Benzoylecgonine COCQB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre (Part Of Serum Cocaine requisition for send out.

Division of Pre and Post Examination, Page 41 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Battery) DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Benzoylecgorine Benztropine (Cogentin) BNZTB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Beta 2 Glycoprotein Screen B2GP1 1 SST Gold top 2.0 mL serum. Freeze. Send on ice pack VGH Lab

Beta-2-Microglobulin B2MB 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory Hospitals In-Common Laboratory Beta-2-Microglobulin NCODE Random urine Collect and submit a fresh urine collection: ask patient to void in a 10 days Y Inc. (Random Urine) sterile container. Ask patient to wait until Tech checks pH of urine 57 Gervais Dr. to ensure pH is above 6.0. If pH is below 6.0, then ask patient to North York, ON M3C 1Z2 drink a glass of water and then one hour later collect urine again.

Copy of requisition for Sendout. Phone 416-391-1499 Ext.248 Check pH and if necessary adjust to pH 6.0 – 8.0 using 1M Fax Phone 416-385-1957 NaOH. Β-2-microglobulin is unstable in acidic urine. Store and send frozen on dry ice. If specimen thaws, it is unsuitable for analysis.

Division of Pre and Post Examination, Page 42 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Beta-2-Transferrin B2TB • Body Fluid – Collect nasal fluid by bending head toward chest and Beta Y SPH Special Chemistry (CSF Leak Investigation, Nasal, ear or allowing clear fluid to drip from nose into sterile container. trace Beta Trace, Beta 2 Mon - other Drainage Fluid must be delivered to lab ASAP. If fluid is collected off site, transferrin) Fri (as much as fluid must be sent frozen on ice pack. Collect blood specimen possible 10µL when fluid is collected. Send serum at 2-8 oC B2 transf minimum) 1 day Must provide Fluid type during Sunquest Order Entry; at • after 1 SST Gold top Result Entry field: Beta or RED top B2TSS=;fluid type (e.g. ;Nasal fluid, ;ear drainage etc…) trace serum (1 mL Do NOT put CSF as the fluid type. minimum) Hospitals In-Common Laboratory Beta-D-Glucan Fungitell NCODE 1 SST Gold top Do NOT aliquot specimen. Collect 3-5mL blood in a serum 5 Days Inc. separator gel tube (SST), centrifuge specimen within 2hrs of 57 Gervais Dr. collection. Store and ship serum gel tube frozen. North York, ON M3C 1Z2 Stability: Refrigerated 5 days. Frozen: 30 days (store and send frozen) Phone 416-391-1499 Ext.248 Copy of requisition for Sendout. Fax Phone 416-385-1957 HICL forward to Viracor-IBT Laboratories Beta Galactosidase, WBC BGAL 1 DRK GRN top Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room Y Children’s Hospital – Biochemical Lithium or Na Hep temperature. Copy of requisition for Sendout. Collect Monday – Genetics Lab Thursday only or consult Supervisor. Room 2F22 *whole blood* SPH Sendout person: 604-875-2307 Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Division of Pre and Post Examination, Page 43 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Beta-Glucosidase, Bloodspot BGLUB NBS Card Optimal 4 spot, minimum 1 spot. Y Children’s Hospital – Biochemical Genetics Lab Beta Glucocerebrosidase, Whatman 903 Blood Preferred: Collect using syringe and drip blood on blood spot 604-875-2307 B-Glucocerebrosidase, spot card card. Gaucher Disease Acceptable: Collect 1 – 2 mLs EDTA blood and transfer to blood spot card. EDTA blood must be transferred to blood spot card within 6 hrs of collection. If using finger/heel prick, allow blood to drip rather than touching blood spot card with patient’s finger/heel.

Obtain minimum one completely filled circle that is soaked through the back of card. Identify name of test on blood spot card. Allow blood spots to dry completely on flat surface for minimum 4 hrs. Do not expose to heat or direct sunlight. Do NOT use the pneumatic tube system to transport wet bloodspot cards. Wet bloodspot cards must NOT be packaged in biohazard bags. Once dry, place blood spot card in sealed plastic bag with a sachet of desiccant (if available). Store in 4°C if there is delay in shipping. Ship at room temperature by overnight courier to Specimen Receiving 2J20. Inform lab at 6048752307 to expect the sample.

Send copy of requisition with transport batch. Beta-Glucosidase, WBC BGLU 1 DRK GRN top 3 mL minimum. Refer patient to Children’s Hospital Lab for blood Y Children’s Hospital – Biochemical Lithium or Na Hep collection if possible. Test is only performed if BGLUB is Genetics Lab Gaucher Disease, Beta abnormal; pre-Approval by Children’s BGL Dr Hilary Vallance Room 2F22 Glucocerebrosidase, B- required and must be indicated on requisition. Glucocerebrosidase, 604-875-2307 Blood must be received at Children’s BGL by 14:00 same day, call 604-875-2307 to inform the Lab. Send copy of requisition with transport batch.

Division of Pre and Post Examination, Page 44 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Beta Glucuronidase, WBC BGLR 1 DRK GRN top Prior consultation and approval by BCCH Lab Biochemical Y Children’s Hospital – Biochemical Mucopolysaccharidosis type Lithium Hep Geneticist is required. Genetics Lab VII, Sly syndrome, MPSVII Room 2F22 *whole blood* Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room temperature. Copy of requisition for Sendout. Collect Monday – 604-875-2307 Thursday only or consult Supervisor. SPH Sendout person: Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. Beta Hydroxy Butyrate BOHB 1 PST Light green 0.5 mL lithium heparin plasma. Separate plasma from cells 24 Hour SPH Chem (ketone) ASAP. Testing needs to be done STAT. Sample stability is 12 hours refrigerated. Referring sites need to aliquot and send

frozen. Beta Mannosidase, WBC BMAN 1 DRK GRN top Minimum 3 mL whole blood. Keep at room temperature. Collect Y Children’s Hospital – Biochemical Lithium Hep and hand deliver to Sendout person before 08:30 Monday – Genetics Lab Friday or consult Supervisor before collection. Copy of requisition Room 2F22 *whole blood* for Sendout. 604-875-2307 SPH Sendout person: Send specimen to Children’s without approval with 09:30 Dynamex Courier. Then give copy of requisition for approval with “Specimen already sent to CW” written on requisition. Specimens collected after 09:30: Seek approval before sending. Same day shipping by taxi, to arrive at Children’s Hospital Lab by 12:00 hrs, (no later than 13:00 Mon-Fri, DO NOT use CW Courier).

1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package.

Division of Pre and Post Examination, Page 45 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Beta Thalassemia MDT 6 mL LAV top Refrigerate. Send on ice pack. Send copy of requisition with N - if ordered Children’s Hospital by Drs.: (Do not transport batch. Ezzat, use Ordering Dr must complete Molecular Genetics Laboratory C&W Foltz BTHCW requisition: S. Jackson, code) http://www.elabhandbook.info/phsa/Files/RequisitionForms%2f1 C.Leger, _20140722_042203_CWMG_REQ_0000_v4.2_General_Requis H.Leitch, ition%20edit.pdf Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Bethesda Units (Factor 8) F8AB 3 BLUE top citrate Full citrate draw. Specimen must be centrifuged within 2 hours Once per Y SPH Special Coag. double spun of collection. week 3.0 mL plasma double spun, aliquot in polypropylene tube. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy of requisition for Special Coagulation

Bethesda Units (Factor 9) F9IB 3 BLUE top citrate Full citrate draw. Specimen must be centrifuged within 2 hours Once per Y SPH Special Coag. double spun of collection. week 3.0 mL plasma double spun, aliquot in polypropylene tube. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy of requisition for Special Coagulation.

Miscellaneous BGL Test BGLMSO Collect LAV top in Plasmalogen is only performed upon approval by CWH Y Children’s Hospital amount specified Biochemical Genetics doctor. on requisition 1. To be used for rare biochemical tests for which there is no Preferable test code. The clinician has usually consulted with BGL as collection on to what to collect and should have indicated the tube type Monday morning on the requisition (MSP, IP or any Outpatient req. If request was made on CWH Molecular Genetics Lab requisition, order MDT only). 2. Enter “type of specimen” and “test requested” where prompted at order entry

Division of Pre and Post Examination, Page 46 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Bile Acids Assay BILE 1 DRK GRN top Whole blood preferred. Minimum 0.5 mL (non gel Lithium Children’s Hospital (Bile Salts, Total Bile Acids) Lithium Hep Heparin), serum from red top tube is acceptable. Must fast for 8 Specimen Receiving 2J20 *whole blood* hours minimum. Centrifuge and separate minimum 200 uL plasma or serum. If same day collection transport at 4 °C, if not same day collection transport frozen on dry ice. This test is restricted to pregnant patients. Order UBILE for non-pregnant patients

Bile Acids, Urine UBILE Random Urine Aliquot 2 – 5 mL urine. Freeze and send frozen Children’s Hospital

Bile Pigments, urine RTU or Random urine Must be fresh sample, received and tested in Lab within 12 hours 24 Hour SPH Urinalysis (urine Bilirubin) of collection. RMU

Bilirubin (Neonate) – Total TBIL 1 PST Light green Minimum 0.1 mL lithium heparin plasma, protect from light. 24 Hour SPH Chem top microtube part of <7 days old: order TBIL (BUCP) 7-365 days old: order BUCP (TBIL + BUC)

Bilirubin (Neonate) – Direct BUC 1 SST Gold top >200 µL serum or lithium heparin plasma. Protect from light, and Children’s Hospital <365d microtube send frozen. If TBIL is elevated, send BUC STAT to BCCH. 604-875-2303 part of or Contact Children’s Lab that sample is being sent. (BUCP) 1 PST Light green Outpatients: order BUCP (TBIL + BUC) top microtube

Bilirubin – Cord CBIL 1 SST Gold top or 0.5 mL minimum collected by nurse from Cord blood. 24 Hour SPH Chem PST Light green top

Bilirubin – Direct DBIL 1 SST Gold top 0.1 mL lithium heparin plasma or SST serum. 24 Hour SPH Chem Neonate >366 Days microtube

Bilirubin (adult) – Total TBIL 1 PST Light green 0.5 mL plasma. 24 Hour SPH Chem. top

Bilirubin – Total, Fluid FTBIL PST, SST, RED 0.5 mL fluid. 24 Hour SPH Chem FTYPE top or non FTYPE: PLEUR; DIA; PERIT; BAL; PCF preservative Synovial fluid TBIL not available. container

Biopterin BIOP Random urine Protect urine from light with aluminium foil. Freeze and send Children’s Hospital wrapped in foil frozen. Send copy of requisition with transport batch. Urine Pterins, Neopterin

Division of Pre and Post Examination, Page 47 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Biotinidase, Serum BTN 1 RED top on ice. Optimal: 3.0 mL, minimum 1.0 mL serum. Freeze Serum –70°C. Y Children’s Hospital – Biochemical Ship serum frozen, same day or overnight, on dry ice to Genetics Lab Specimen Receiving 2J20. Room 2F22 Send copy of requisition with transport batch. 604-875-2307

BK Virus PCR, plasma PPOLY 6mL LAV EDTA Minimum 3 mL plasma. After hour and referring Labs, aseptically Mon – SPH Virology Lab (Polyomavirus PCR, BKV separate within 4 hours of collection into sterile plastic cryovial. Fri PCR, JC virus) Freeze at –20c. Send Frozen. Results in 3 days * Patients <19 years old are to CBKVL 1 RED top 2 mL RED top whole blood. Patients <19 years old are to be sent Children’s Hospital Virology be sent to BCCH.* to BCCH. Samples must be sent, on ice pack, Monday to Friday 800 to 2300 or Saturday, Sunday and Statutory Holidays 800 to 1600. Specimen must be received within 48 hours of collection.

BK Virus PCR, non blood PVPCR Sterile urine Refrigerate sample and send on ice pack. Sample must be Y SPH Virology Lab (Urine BK virus, Urine BKV, container accompanied by frozen EDTA plasma sample collected on same Urine Polyomavirus PCR, JC day. Copy of requisition for Virology. Med Micro approval 1.0 mlL CSF virus PCR urine, JC virus required. CSF, BK virus CSF) Blastomyces Dermatitidis Ab BLASB 5 mL SST Gold top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control Modify BCCDC’s Specimen Description with Med Micro initials: by Medical C/O PHSA Laboratories – Lane E.g. SD0163=BLD-AMMMR Microbiologist Level Laboratory

Blastomycosis-Serology BLASB 5 mL SST Gold top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Blastomyces Dermatitidis Modify BCCDC’s Specimen Description with Med Micro initials: by Medical C/O PHSA Laboratories – Lane Ab) E.g. SD0163=BLD-AMMMR Microbiologist Level Laboratory

Bleeding Time Test no longer available.

Blood & Body Fluid Exposure PBBFE 5 mL SST Gold top Includes PHBSAG, PHBSAB, HIVCA, PHCV, and PHBCAB SPH Virology Lab Viral Serology (Occupational 1. Do NOT cancel or Credit the SCM PHCE order in Sunquest. Exposure, Needlestick) 2. Login PBBFE test code with PEHU-PHN (at SPH) or MEHU- PHN (at MSJ) as Patient ID (MRN). 3. Label samples with PBBFE barcode.

Blood Culture PBLOD 1 FA Plus and Green and Orange bottle. 2-7days SPH Microbiology 1 FN Plus set

Blood Culture, Fungus PFBLOD 1 FA Plus Green bottle. SPH Microbiology

Division of Pre and Post Examination, Page 48 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Blood Culture, PISOL Yellow Lysis tube These tubes are only available from Microbiology between 06:00- SPH Microbiology Isolator/Histoplasma 23:00.

Blood Culture – Pediatric PBLPED PF Plus Yellow bottle for Pediatric Collections. 2-7days SPH Microbiology

Blood Culture PTBBC BD BACTEC Narrow neck bottle. Second set not allowed. Only 2 sets in 6 6 weeks SPH Microbiology (TB, MAC, Mycobacterium) weeks permitted.

Blood Gas, Arterial BGASP Syringe, balance >250 µL whole blood without air bubbles. Deliver to Core Lab STAT SPH Chemistry Heparinized immediately.

Blood Gas, Capillary Capillary blood in 125 µL whole blood without air bubbles. Put on ice and deliver to glass rod. Core Lab immediately. NICU babies for Lactate: Add BLACT to BGASP battery.

Blood-Grouping Transplant Two 6 mL LAV top Red cell transfusion request form. VGH Lab Society

Blood Parasite Referral RBPREF 1 LAV top and Order on new accession # by Hematology only. BC Centre For Disease Control (Malaria Confirmation Thick and Thin C/O PHSA Laboratories – Lane Ordered By Hematology) slides. Level Laboratory – (Regionally Assisted Testing – RAT)

Blood Smear AMOR 1 LAV top 1.0 mL minimum draw for 3 mL EDTA tubes. Slide must be 24 Hour SPH Hem. (RBC Morphology) prepared within 6 hours of collection.

Blood Urea Nitrogen URE 1 PST Light green 0.5 mL lithium heparin plasma. Send on ice pack. 24 Hour SPH Chem (BUN, Urea) top 0.5 mL fluid. Synovial fluid urea not available. Blood Urea Nitrogen, fluid FURE 1 PST, SST, RED 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF (Fluid urea) top or non

Division of Pre and Post Examination, Page 49 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 1. Package using supplied Fedex Pak and complete pre Blueprint Cardiology Genetic NCODE 1LAV top included Attn: Tero-Pekka Alastalo printed Fedex Waybill, bill to recipient Fedex Acc test Kit in Kit (Order #249659606. Complete Commercial Invoice and Pro Blueprint Genetics Inc. RES1 or Forma Invoice (provided in Kit). 953 Indiana Street RES2 etc 2. Put specimens in Transport Batch, specify “SPH to for any Blueprint” in Batch. Put Fedex tracking number on Batch San Francisco, CA 94107 U.S.A. extra sheet. Store batch sheet in sendout binder in GeneDx tab. Phone: (650) 452-9340 labels 3. Specimens stable for 7 days refrigerated. Batch needed) specimens and send at Room Temp. within 3 days of collection. Do not send on Friday. 4. Batch and package maximum 3 kits in 1 Fedex Pak, or in larger TDG container if more than 3 kits received; ship each Pak or container using 1 Fedex Waybill. Blueprint Genetics - no kit NCODE 6 mL LAV top Minimum 1.0 mL Whole Blood. Copy of requisition for Sendout. Y Children’s Hospital Molecular Genetics Lab (MGL) (to be sent to BCCH) Send at RT Ordering Dr must complete Molecular Genetics Laboratory C&W 4500 Oak Street requisition and Blueprint Genetics requisition. Vancouver, BC Specimen Receiving Room 2J20 Sample to be sent to California by BCCH. Bill73 consent form must be signed

Bone Gla Protein (Osteocalcin) NCODE 1 RED top Minimum 1.0 mL serum from Red or gel tube. Stability: 72 hours Y Hospitals In-Common Laboratory refrigerated; 3 months frozen. Copy of requisition for Sendout Inc.

bench. 57 Gervais Dr. Avoid hemolysis. Spin and separate immediately and split North York, ON M3C 1Z2 into 2 aliquots. Freeze immediately. Send frozen on dry ice. Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

Borrelia By PCR BBNATB 6 mL LAV top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Borrelia Burgdorferi Nat) by Medical C/O PHSA Laboratories – Lane CSF for Borrelia PCR must be accompanied by 5 mL SST Gold (Lyme Disease PCR) Microbiologist Level Laboratory – Test Requiring *5 mL SST Gold top tube (serum used to screen for Borrelia first). Approval (TRAP) top, CSF, Fluid Specify specimen type in SPEC specimen description. acceptable * Modify BCCDC’s Specimen Description with Med Micro initials: E.g. SPEC33=BLD-AMMMR

Borrelia Serology (Lyme BBGMS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Disease serology) C/O PHSA Laboratories – Lane Serology is NOT performed on CSF. Level Laboratory

Division of Pre and Post Examination, Page 50 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Borrelia Burgdorferi Serology BBGMS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

BORRELIA BURGDORFERI BBG 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control IgG C/O PHSA Laboratories – Lane Level Laboratory

BORRELIA BURGDORFERI BBM 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control IgM C/O PHSA Laboratories – Lane Level Laboratory

Borrelia hermsii IgG IFA BHG 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Relapsing Fever Serology) C/O PHSA Laboratories – Lane Level Laboratory Brancher Enzyme NCODE 2 DRK GRN top Requires consultation. Please refer to children’s hospital Y Children’s Hospital – Biochemical Lithium Hep or Na handbook for complete instructions. Copy of requisition for send Genetics Lab Hep out. Room 2F22 *whole blood* 604-875-2307

Breath Test For H. Pylori Breath bag kit Refer patient to Lifelabs Lifelabs

Bromazepam (Lectopham) BROMB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Bromazepam Bromide NCODE No longer available. Updated Sept. 2011 Y

Brompheniramine (Dimetane) BROPB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 51 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Brucella Serology BRUCB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Brucella Abortus Serology) C/O PHSA Laboratories – Lane Level Laboratory

BSP (Bromosulphth Alein) Not available

Bupivicaine SPQ 1 RED top 2-4 mL RED top serum. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; BUPIVICAINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

DRSCB 2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Bupivicaine

Buprenorphine BUPB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 52 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

(Suboxone) DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Buprenorphine Bupropion BUPRB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Bupropion Burkholderia Pseudomallei BURKAB 5 mL SST Gold top Refrigerate. Send on ice pack. Test is sent to Reference Lab by BC Centre For Disease Control Serology (Melioidosis) BCCDC.

Buspirone SPQ 1 RED top 2-4 mL RED top serum. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; BUSPIRONE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 53 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Butabarbital (Butisol) BUTAB 1 RED top 2-4 mL serum. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Hospitals In-Common Laboratory C2 Complement NCODE 1 SST Gold top 1.0 mL serum frozen. Send on dry ice Y Inc. Put Fax Result to SPH Lab request sticker on requisition or on 57 Gervais Dr. Sunset Printout. North York, ON M3C 1Z2

Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

C3 Complement C3 1 SST Gold top 0.5 mL serum frozen. Send on dry ice. Mon – SPH Special Chem Fri

C4 Complement C4 1 SST Gold top 0.5 mL serum refrigerated. Mon – SPH Special Chem Fri

Division of Pre and Post Examination, Page 54 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

C3C4 C3C4 1 SST Gold top 0.5 mL serum frozen. Send on dry ice. Mon – SPH Special Chem Fri

C5 Complement, Functional, NCODE 1 RED Top on wet Immediately after drawing the specimen, place the tube on wet Y Specimen Process Centre Serum ice. ice. Spin down and separate serum from clot. Immediately freeze Mayo Medical Laboratories specimen. Fasting preferred. 1.0 mL serum, minimum 0.5 mL. 3050 Superior Drive NW Mayo Test ID: C5FX Stability 14 days frozen. Ensure Bill 73 is completed and copy Rochester, MN 55901 of requisition for Sendout bench. 1-800-533-1710 SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. C-Peptide CPEP 1 SST Gold top Mon – SPH Special Chemistry Minimum volume 120 uL serum in False Bottom or 13 x 75 FAST Fri Polypropylene tube, Freeze. 10 hour fasting specimen preferred. Send frozen with dry ice, must arrive at SPH frozen. Non-fasting requests +/- Glucose result from other labs: (phone referring lab if fasting status is not indicated on requisition and ask status and Glucose result.) Reminder: a FPG result is FASTING!  If glucos e re s ult NO T a va ila ble → order CPEP but do NOT give requisition to Special Chem.  If glucose result available, record glucose on requisition → order CPEP and give copy of requisition to Special Chemistry

C-Reactive Protein CRPB 1 PST Light green 1.0 mL plasma. Refrigerate. Send on ice pack within 3 days of 24 Hour SPH Chem Lab. top collection or longer if frozen.

CA 125 Cancer Antigen 125 CA125B 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

CA 15-3 Cancer Antigen CA15- CA153B 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control 3 C/O PHSA Laboratories – Lane Level Laboratory

Division of Pre and Post Examination, Page 55 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

CA 19-9 Cancer Antigen 19-9 CA19B 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory Hospitals In-Common Laboratory Cadmium, Blood CADMB 1K2EDTA Dark Prior arrangement with Laboratory is mandatory to ensure 10 days Y Inc. blue top collection tube supply is available. Test is not covered by 57 Gervais Dr. MSP, prior approval required for all Outpatients or Contact *Special Collection North York, ON M3C 1Z2 HICL for current fee ($42.50) if patient wants to self pay. tube from Supervisor* Do not spin. Store in fridge; send on ice pack as whole blood. Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 http://www.hicl.on.ca/search_tcna.asp?TCString=HCAD%20 WB Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Children’s Hospital? Cadmium, Urine CDRU Random Urine, 50 mL urine, collect and transfer in metal-free Y

metal free container. Indicate “Random”. Copy of requisition for Hospitals In-Common Laboratory container Sendout. Performed at CW if an emergency only such as suspected Inc. poisoning and after approval by CW clinical chemist 57 Gervais Dr. North York, ON M3C 1Z2 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 Caffeine CAFFB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Caffeine Calcitonin CLCT 1 RED top on ice Collect on ice, spin cold and freeze 0.5 mL serum immediately in Mon & SPH Special Chemistry False Bottom or 13 x 75 Polypropylene tube. Must arrive at Thurs SPH frozen.

Calcium CA 1 PST Light green 0.5 mL lithium heparin plasma. 24 Hour SPH Chem top

Division of Pre and Post Examination, Page 56 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Calcium – Fluid FCA 1 PST, SST, RED 0.5 mL fluid. Synovial fluid Calcium not available. 24 Hour VGH top or non FTYPE: PLEUR; DIA; PERIT; BAL; PCF preservative container

Calcium, Ionized CAI 1 SST Gold top Must be at least a ½ full draw. Do not open tube. Put tape over 24 Hour SPH Chem (Free Calcium) stopper. Send spun on ice pack.

Calcium and Phosphorus CAPO4 1 PST Light green 0.5 mL lithium heparin plasma. 24 Hour SPH Chem top Refrigerate during collection. Measure 24 hour volume. Aliquot Calcium, 24 hour urine CAU 24 hr urine, no Mon – SPH Chem 3 mL for urine creatinine, centrifuge and aliquot supernatant to 12 preservative. (Acid Fri Add CRU, x75 plastic tube. Aliquot approximately 100 mL from well mixed container UTIM 24 hour collection and adjust the pH of aliquot to pH 2-3 with 6 M acceptable) HCl (do not send whole collection) for heating at SPH. Referring sites need to include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack. Calcium, Random Urine CAR Random urine Order code includes urine creatinine and ratio. 3 mL urine, 24 Hour SPH Chem centrifuge and aliquot supernatant to 12 x75 plastic tube. Prior approval required and patient (patient Dr.) to provide written Calcium Sensing Receptor NCODE 1 x 6 mL EDTA 3-4 Y MSP approval letter before sample collection. Send at Room Betty Wong Mutation (CASR Gene months temperature within 1 day of collection or refrigerate and send on Sunnybrook Health Sciences Sequencing) ice pack. Stability 3 days at Room Temp, 7 days refrigerated. Center Molecular Genetics Lab Copy of requisitions for send out: 2075 Bayview Ave, E343 Ordering Dr must complete Sunnybrook HSC requisition and Toronto, Ontario M4N 3M5 Canada patient must sign Consent form (Copy and paste path in IE): Phone: (416) 480-6100 x89494 \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Requisitions\Sunnybrook Hospital\Sunnybrook Molecular Genetics Laboratory Requisition.pdf

http://www.genebc.ca/uploads/CWMG_REQ_0210_Coordinating _Out_of_Province_Genetic_Testing.pdf Calculi (Stones) STON Stone in sterile Refrigerate. Assign and send on Transport Batch. Specimen VGH Lab container does not require Order Entry by Anatomic Pathology.

Calculi (Gall Stone) STONG Stone in sterile Refrigerate. Assign and send on Transport Batch. Specimen VGH Lab container does not require Order Entry by Anatomic Pathology.

Division of Pre and Post Examination, Page 57 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Campylobacter PFAEC Stool in sterile Stool for culture and sensitivity 2 days SPH Microbiology container

Campylobacter Serology CMPY 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control TEST NOT AVAILABLE Top tube

Candida-Precipitin Test NCODE 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Cannabinoids In Urine THCSC Random urine >1.0 mL urine. Refrigerate if not done immediately. Order UDP if 24 Hour Y- if DRSCB SPH (THC screen, marijuana) screen ordered. Add DRSCB if Dr specifies THC confirmation ordered Part of (or aka names) and give copy of requisition for Medical UDP Biochemist approval.

Cannabinoids In Urine, DRSCB Random urine Refrigerate. Send 50 mL Urine on ice pack. Y Provincial Toxicology Centre Confirmation Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Cannabinoids confirmation 0.5 mL Lithium Heparin plasma. Ward should record information Carbamazepine Level CARBA 1 PST Lithium 24 hour SPH Chem on dosage, time of last dose and other meds. Specimen should (Tegretol) heparin be collected prior to next dose. Refrigerate. Sunquest Last Dose format: CRBDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE CRBTLD: ;HH:MM e.g. ;13:50 or UNAVOE Carbamazepine Epoxide CEPOX 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 58 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Carbon Dioxide TCO2 1 PST 0.5 mL lithium heparin plasma. 24 hour SPH Chem

Carbon Monoxide COHB 1 unopened EDTA 24 Hour SPH Chem (Carboxyhemogloblin) OR blood Gas Stat Lab Syringe

MSJ – 1 PST or MSJ Lab blood gas syringe

Carboxy-THC SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; CARBOXY THC SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

DRSCB >2mL urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Carboxy-THC

Carcinoembryonic Antigen CEAB 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Carisoprodol CARIB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Carnitine CARNS 1 SST Gold top 1 mL serum. Send frozen on ice pack. Send copy of requisition Y Children’s Hospital – Biochemical (Total And Free) with transport batch. Genetics Lab Room 2F22 604-875-2307 After removing aliquot for CRR, transfer min 2 ml to a 5 ml tube Carnitine Fractional Excretion CFE Random Urine Children’s Hospital and ship frozen Add CRR, CARNS Consult Children’s Hospital before collection. and CRE

CPT1a,P479L Variant CPTVA Blood Dot Card Optimal: 4 spots, minimum: 2 spots. For small babies, 2 spots Y Children’s Hospital (NBS 903 filter on bloodspot card are sufficient for both acylcarnitine profile CPT1a P479L variant, CPT1 card) (bloodspot) and CPT1a P479L variant. P479L mutation, CPT1 Identify both requests clearly on card. molecular, CPT1 P479L molecular, CPT1, CPT1 Do NOT use the pneumatic tube system to transport wet mutation, CPT1a P479L bloodspot cards. Wet bloodspot cards must NOT be packaged mutation, Carnitine Palmitoly in biohazard bags. Transferase mutation Send copy of requisition with transport batch.

Carotene CARO 1 SST Gold top 2 mL serum. Protected from light (wrap in foil), freeze. VGH Lab wrapped in foil

Catecholamines (Plasma) CATP 2 x 6 mL pre- DO NOT COLLECT in Outpatients – physician needs to book Y Special Chemistry (Adrenaline, Dopamine, cooled EDTA. through Medical Day Care (MSSU). Blood should be drawn in VGH Lab Epinephrine, Noradrenaline, Collect/transport the morning through an indwelling venous catheter after 30 Pressoramines) on ice. minutes of rest in a supine position. Patient shold be fasting. Collect in two precooled EDTA tubes (7mL) lavender top. Transport to the lab on ice within one hour of collection. Centrifuge at 4°C. Require minimum 5 mL plasma separated into 2 X 2.5 mL aliquots and freeze immediately in - 70°C freezer. Send frozen on dry ice. Catecholamines (24 hr. Urine) CATEU 24 HR urine Collect in collection bottle containing 15mL of 6N HCL. If not Y – If more VGH Lab (Epinephrine, collection in 15 mL collected in acid, acidifiy in lab, to pH 2 - 4, only if entire collection than 1 of 3 Add CRU, Norepinephrine and of 6 Mol/L HCL is received. Acifidication must be performed within 12 hours tests ordered Dopamine) UTIM after completion of 24 hr urine collection. Aliquot 50 mL of a well- CAT, VMA or mixed 24hr collection, refrigerate, send on ice pack. UMETA. If

Division of Pre and Post Examination, Page 60 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) only 1 of 3, no Catecholamines, Random urine CATER 50 mL aliquot of Acidifiy in lab to pH 2 - 4. Acifidication must be performed approval random urine within 36 hours of random urine collection. Minimum 12 mL. needed. Refrigerate. Send on ice pack. Cathinones, Urine DRSCB 10 mL random Refrigerate. Send on ice pack Y BCCDC Prov Tox (Bath Salt) urine Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Cathinones

CD3 CELLS CD3CT 1 LAV AND 1 DK Must also order CBCD. Room temp. Copy of req to SPH Mon – SPH Immunology (only use this code if other add GRN Na Heparin immunology. Fri CD NOT ordered, otherwise CBCD use IDEF or IMMCM)

CD4 HSPP 1 DK GRN Na Must also order CBCD. Room temp. Copy of req to SPH Mon – SPH Immunology add Heparin or ACD A immunology. Collect 2 EDTA Monday to Friday until noon. After Fri CBCD plus noon on Friday, weekends and holidays collect 1 ACD A and 1 1 LAV EDTA.

CD8 HSPP 1 DK GRN Na Must also order CBCD. Room temp. Copy of req to SPH Mon – SPH Immunology add Heparin or ACD A immunology. Collect 2 EDTA Monday to Friday until noon. After Fri CBCD plus noon on Friday, weekends and holidays collect 1 ACD A and 1 1 LAV EDTA.

CD4/CD8 Ratio HSPP 1 DK GRN Na Must also order CBCD. Room temp. Copy of req to SPH Mon – SPH Immunology (Helper/Suppressor ratio) add Heparin or ACD A immunology. Collect 2 EDTA Monday to Friday until noon. After Fri (H/S ratio) CBCD plus noon on Friday, weekends and holidays collect 1 ACD A and 1 1 LAV EDTA.

CD19 (Must Include One Of IDEF 1 DK GRN Na Room temp. Copy of req to SPH immunology. Must also order Mon – SPH Immunology CD3, CD56, CD57) add Heparin or ACD A CBCD Fri CBCD plus 1 LAV CD19/CD20 IMMCM, 1 DK GRN Na Drug Monitoring for Rituximab. Must also order CBCD. Room Mon – SPH Immunology add Heparin or ACD A temp. Copy of req to SPH immunology. Collect 2 EDTA Monday Fri CBCD plus to Friday until noon. After noon on Friday, weekends and holidays 1 LAV collect 1 ACD A and 1 EDTA.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

CD19/CD25 CAAP 1 DK GRN Na Send room temp. Must also order CBCD. VGH Lab Heparin plus add 1 LAV CBCD 2 DK GRN Na CD38 IMMCM Copy of requisition to immunology. Store at room temp. Must also Mon – SPH – Immunology Heparin or ACD A add order CBCD. If collection is on Friday, consult immunology tech. Fri plus CBCD Test available Mon-Fri. –updated Mar31/05.KL 1 LAV

CD56 IDEF 1 DK GRN Na Room temp. Copy of req to SPH immunology. Must also order Mon – SPH Immunology add Heparin or ACD A CBCD Fri CBCD plus 1 LAV CD57 IDEF 1 DK GRN Na Room temp. Copy of req to SPH immunology. Must also order Mon – SPH Immunology add Heparin or ACD A CBCD. Fri CBCD plus 1 LAV Ceruloplasmin CPL 1 SST Gold top 0.5 mL serum. Tues/Fri SPH Special Chem

CH 50 CH50 1 RED top in Clot @ 37°C for 30 minutes followed by 1 hour at 4°C. Centrifuge Y – all others VGH Lab (Classical & Alternative) ° warm (37 C) water at 4°C. For 15 mins. Separate into two plastic tubes. N – no (Complement, Total) bath. Freeze at –70°C.(Immunopath. Freezer). Send specimen on dry approval for (Complement, Total Pre-approval ice. Copy of requisition for send out. OP. Hemolytic) required before (Immunology Profile) collection. Chikungunya Virus Serology CHIK 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Chitotriosidase CHITO 1 RED top Separate in 2 aliquots and freeze Serum immediately. Send Y Children’s Hospital – Biochemical frozen. Clinical history must be Gaucher’s disease. Done 4x a Genetics Lab (Acid Phosphatase) year. Only perform in first week of March, June, September, and Room 2F22 December. Check preapproved patient list. Send copy of 604-875-2307 requisition with transport batch.

Division of Pre and Post Examination, Page 62 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Chlamydia/GC swab PCTNGS Chlamydia swab Store and send at room temp. Mon – SPH Microbiology Fri Sunquest Order Entry instructions: Must input swab site code in second screen: 24 hours to result. SDES Specimen Site Code Translation

UR Urethra

CX Cervix or Intracervix

ECX Endocervix

GENIT-SNG Genital SITE NOT GIVEN (copy of req.)

RECTSW Rectal Swab (copy of req.)

VA Vagina (copy of req.)

MISCSP-;xxxx site All other sites (copy of req.)

If CX and ECX both checked on req., use ECX site code.

Chlamydia/GC Swab, Eye DSCG Chlamydia Swab Store and send at room temperature. BCCDC Microbiology (Swab for CT/GC NAT) on eyes. Sunquest Order Entry: (Chlamydia Gonorrhea Nat SD0039: EYE (change the default Cervix CX to EYE) Swab)

Chlamydia/GC urine PCTNGU Chlamydia urine in Store refrigerated, send on ice pack. Mon – SPH Microbiology sterile container Fri 24hr to result.

Chlamydia Serology Includes: CHLGRP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Chlamydia Pneumoniae IgG C/O PHSA Laboratories – Lane Chlamydia Pneumoniae IgM Level Laboratory Chlamydia Psittaci IgG Chlamydia Psittaci IgM Chlamydia Trachomatis IgG Chlamydia Trachomatis IgM

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Chloral Hydrate DRSCB 1 RED top or 2-4 mL RED top serum or urine and refrigerate. Send on ice pack. Y Provincial Toxicology Centre Copy of requisition for send out. >2 mL urine Sunquest LIS Order Entry:. Second screen: DRSC1 =;Chloral Hydrate

Chloramphenicol CHPNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 64 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Chloride, 24 hour urine CLU 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem preservative. hour collection (do not send whole collection) centrifuge and Fri Add CRU, (Unsuitable if aliquot supernatant to 12 x75 plastic tube. Referring sites need to UTIM collected in Acid.) include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack.

Chloride, random urine CLR Random urine 3 mL aliquot of random urine, centrifuge and aliquot supernatant 24 Hour SPH Chem to 12 x75 plastic tube.

Chloroquine (Aralen) CHLQB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

Division of Pre and Post Examination, Page 65 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

or Sunquest Order Entry instructions for Urine samples: 20 mL Random Urine (see  At Container and Specimen Entry: remove “R” and add “UR” Instructions)

Chlorthalidone (Cobipress) Not available

Cholestanol CHO 1 Green Top Na Centrifuge and aliquot minimum 1 mL heparin plasma, freeze and Y Children’s Hospital Heparin on ICE send frozen. Send copy of requisition with transport batch. Put C&W will forward. Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Cholesterol – Fluid FCHOL 1 PST, SST, RED 0.5 mL fluid. 24 Hour SPH Chem top or non FTYPE: PLEUR; DIA; PERIT; BAL; PCF preservative Synovial fluid cholesterol not available. container

Cholesterol, Total serum CHOL 1 SST Gold top 0.5 mL serum. For fasting status enter PF-;Fasting for XX hours. 24 Hour SPH Chem For example, if the patient came in for bloodwork at 1000 and they had eaten at 0800 you would enter “PF-;Fasting for 2 hours”. If the fasting status is unknown because the sample has been dropped off or sent in and the patient is unavailable to ask when they last ate enter PF-UNAVOE.

Division of Pre and Post Examination, Page 66 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cholesterol Profile CHOLP 1 SST Gold top 0.5 mL serum. 24 Hour SPH Chem No triglyceride (non-fasting) For fasting status enter PF if patient has been fasting 10-14 hours. If the patient has been fasting for less than 10 hours (or not at all) enter ;Fasting for X hours. For example, if the patient came in for bloodwork at 1000 and they had eaten at 0800 you would enter ;Fasting for 2 hours”. If the fasting status is unknown because the sample has been dropped off or sent in and the patient is unavailable to ask when they last ate enter UNAVOE.

Cholinesterase And Dibucaine PSDCS 1 SST Gold top 1 mL serum. 24 hr storage – fridge Y Royal Columbian Hospital via ACE Floride Number >24 hr at –20°C. Copy of requisition for send out. Put Fax Courier (Pseudocholinesterase) Result to SPH Lab request sticker on requisition or on Sunset Printout.

Cholinesterase – RBC and 1 EDTA whole Mix thoroughly by gentle inversion. Lavender top tube MUST be Y Lifelab Cholinesterase – Plasma blood centrifuged for 15 minutes WITHIN 30 minutes of collection. (Exposure To Using a plastic pipette transfer ALL the plasma to a labeled Organophosphate Pesticides aliquot tube and cap tightly. Save the remaining tube containing Or Toxicity) Red cells with and cap tightly. Hemolyzed specimens are unacceptable for analysis. Elasticize both tubes together and send both aliquot tube and collection tube. Refrigerate. DO NOT FREEZE Copy of requisition for send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Hospitals In-Common Laboratory Chromium, Whole Blood CHRWB 1 K2EDTA Dark Prior approval required for all Outpatients; ensure collection Y Inc. Blue top tube supply is available. 57 Gervais Dr. (Tubes in 4 mL whole blood. Do not open tube or separate. Store and send North York, ON M3C 1Z2 Technical cold. Coordinator’s Phone 416-391-1499 Ext.248

office) Fax Phone 416-385-1957

Collect and transfer in metal-free container. Provide Hospitals In-Common Laboratory Chromium, Urine CHRR Random urine 10 days date of birth and gender. Indicate “Random”. Avoid Inc. mineral supplements for 5 days. 57 Gervais Dr. Performed at CW if an emergency only such as suspected North York, ON M3C 1Z2 poisoning and after approval by CW clinical chemist. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Phone 416-391-1499 Ext.248 Printout. Fax Phone 416-385-1957 Division of Pre and Post Examination, Page 67 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Chromogranin A CGAB 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Chromosome Analysis (Bone CGMVH BONE MARROW Must send STAT on the same day. Samples will be coming from VGH Cytogenetics Lab Marrow) SOLUTIONS Hematology department. JPS1 Rm 1800 855 West 12th Avenue Use Dynamex courier for every STAT.

Cytogenetics Lab – Children’s Hosp Chromosome Microarray CCGM 2 x 3.0mL EDTA Keep at room temperature. Send ASAP (within 48 hours) to Dept of Pathology & Lab. Medicine Testing (do not use CMAR whole blood. Children’s Cytogenetics Lab with copy of original requisition. 4480 Oak Street, Vancouver BC code) Test available only to some Drs. V6H 3V4. Tel: 604 875 2304

Division of Pre and Post Examination, Page 68 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Chromosome Studies CGBA ADULTS: 1 DRK Copy of requisition for sendout. N - if ordered Adults: (Karyotype) GRN Na Heparin by Drs.: Send at room temp stat on same day (use dynamex courier if VGH Cytogenetics Lab (Chromosome Analysis- Ezzat, necessary) JPS1 Rm 1800 Peripheral Blood) Foltz th 855 West 12 Avenue S. Jackson,

Adults C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Chromosome Analysis CCGBB NEWBORNS: USE Ordering Dr must have completed Children’s Cytogenetics N - if ordered Children’s Hospital Cytogenetics Lab Newborn 6 mL Sodium requisition found in: by SPH 15q11 Duplication Heparin tube. http://www.elabhandbook.info/phsa/Files/requisitionforms%2f1_20 maternity 110510_022844_Cytogenetics_bloodbiopsy_Req_CW042_v2011. (PEDIATRICI Angelman Syndrome Minimum 0.5 – 1.0 pdf AN) Bloom Syndrome mL blood is Store and send at room temperature, specimen must be received acceptable for Cri-du-chat Syndrome by Children’s Cytogenetics Lab within 72 hours of collection. CMA Follow-up newborns CGH, Comparative Genomic STAT orders from Maternity must be sent STAT, inform Hybridization Children’s Cytogenetics Lab to expect specimen 604-875-2304. DiGeorge Syndrome Send copy of requisition with transport batch. Fanconi Anemia

Karyotype, Karyotyping, Chromosome Analysis, Chromosomes Kallman’s Syndrome Mosaicism Constitutional Miscellaneous CGL Test Miller-Dieker Syndrome XX XY Mosaicism, post BMT Prader-Willi Syndrome Smith Magenis Syndrome SRY Sotos Syndrome Steroid Sulphatase Deficiency Sub-Telomeric Testing TUPLE 1 Duplication Turner’s Syndrome

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Wolf-Hirschorn Syndrome William’s Syndrome Chylomicrons FCHOL/ 1 PST, SST, RED 0.5 mL fluid. 24 Hour SPH Chem (query chylous, pseudochylous FTRIG top or non FTYPE: PLEUR; DIA; PERIT; PCF effuffsion) preservative Login and Accessioning instructions: FTYPE container 1. Pleural fluid: If “chylomicrons” or “query chylous or pseudochylous effusion” requested on fluid, log in FCHOL and FTRIG. 2. Peritoneal Fluid: If “chylomicrons” or “query “chylous ascites” requested on peritoneal fluid, log in FTRIG. 3. Drainage Fluid: If “chylomicrons” or “query chyle” requested on drainage fluid, log in FTRIG.

 Spin and process CID by PDEC, give decanted CID aliquot of fluid with FTRIG and /or FCHOL request to Advia bench.  If FTRIG or FCHOL requested, keep portion of unspun fluid. Label it with non barcoded portion of PDEC label, write “UNSPUN” and store it in Accessioning refrigerator. Chymex Unavailable

Chymotrypsin (Stool) At least 2 gram of Not available at BCCH. See elastase. Newborn (3 To 4 Days Of fresh stool Age)

Circulating Immune Complex CPLX 1 SST Gold top 1.0 mL serum. Separate Serum from clot and freeze within 2 VGH Lab (C1q Binding Assay) hours. Send frozen on ice pack

Citalopram (Celexa) CITALB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Centre requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Citrate, 24 hour Urine CTU 24 hour urine Refrigerate during collection. Measure 24 hr. Volume and aliquot Y – if VGH Lab add CRU, collected either in 100 mL from well mixed 24 hour collection (do not send whole inpatients UTIM acid or without collection). Freeze and send on ice pack. preservative

Citrate, random urine CTR Random urine 10 mL urine. Freeze and send on ice pack. Y – if VGH Lab inpatients

CK (Creatine Kinase) CK 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem (CPK, Creatine Phosphokinase) Hospitals In-Common Laboratory CK-MB Fraction NCODE 1 SST Gold top 0.5 mL Serum. Store and send frozen. Stability: 4 hours at room 3 days Y Inc. (CKMB) – Verify with ordering temperature, 2 days refrigerated, 6 months frozen. Plasma . 57 Gervais Dr. Dr if TROPI can be used. (Heparin) is acceptable but has up to +12.5% bias. North York, ON M3C 1Z2 Additional information on HICL website: http://www.hicl.on.ca/search_tcna.asp?TCString=CKMB Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 Send specimens on dry ice. Copy of requisition for send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

CK Isoenzyme electrophoresis NCODE 1 SST Gold top 2 mL SST serum. Pre-Approval required. Ensure Bill 73 is Y Specimen Process Center (CK Macro, CKBB, CKMM) completed and copy of requisition for Sendout bench. Mayo Medical Laboratories 3050 Superior Drive NW Mayo Test ID: CKELR SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Refer to Mayo Medical Lab: http://www.mayomedicallaboratories.com/test- catalog/Specimen/80906 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Clobazam & CLBZB 1 RED top 2-4 mL RED top serum or urine. Ward should record information Y Provincial Toxicology Center Desmethylclobazam on dosage, time of last dose, and other meds. For therapeutic

drug monitoring specimens must be drawn at trough. Serum (Frisium) must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Clomipramine & CLMPRB 1 RED top 2-4 mL RED top serum or urine. Ward should record information Y Provincial Toxicology Center Desmethylclomi-Pramine on dosage, time of last dose, and other meds. For therapeutic

drug monitoring specimens must be drawn at trough. Serum (Anafranil) must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Clonazepam CLNZB 1 RED top 2-4 mL RED top serum or urine. Ward should record information Y Provincial Toxicology Center on dosage, time of last dose, and other meds. For therapeutic (Rivotril) drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Indicate if for toxicity. Refrigerate. Send on ice pack. If patient of Dr.

Misri, already pre-approved.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Clostridium Difficile Toxin PCDTOX Stool sample in Do not freeze. Results SPH Microbiology sterile C&S in 24 container. hours.

Clot Retraction Unavailable

Clozapine CLZPB 1 RED top 2-4 mL RED top serum or urine. Ward should record information Y Provincial Toxicology Center on dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Note: hematology panel (CBCD) is usually required for monitoring clozapine. Refrigerate. Send on ice pack.

CMV For Buffy Coat 1 ACD (YELLOW Done Mon – Fri before 1100hr. After 11:00hr must phone SPH Virology Lab Replaced by CMVPCR top) tube Virology. Send whole blood specimen.

CMV Genotyping NCODE 6 mL LAV EDTA Minimum 3 mL plasma. After hours and referring Labs, aseptically Y NML via SPH Virology separate within 4 hours of collection into sterile plastic cryovial. (Ganciclovir resistance test) Freeze at –20°C. Send frozen.

CMV IgG (Cytomegalovirus – PCMVG 5 mL SST Gold top Refrigerate. Mon – SPH Virology Lab IgG) Fri twice per (CMV serology, CMV screen) week. Result in 3 days

Division of Pre and Post Examination, Page 73 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

CMV –IgM CMVSP 1 SST Gold top Refrigerate. Send on ice pack. BCCDC

CMV – PCR PCMVB 6 mL EDTA Lav 3.0 mL EDTA plasma. After hours and referring Labs, aseptically Mon – SPH Virology Lab CMV Antigenemia separate within 4 hours of collection into sterile plastic cryovial. Fri CMV Ag Freeze at –20°C. Send Frozen. Results CMV Viral Load in 2 days CMV NAT Cytomegalovirus PCR * Patients <19 years old are to CMVL 1 RED top 2 mL RED top whole blood. Patients <19 years old are to be sent Children’s Hospital Virology be sent to BCCH.* to BCCH. Samples must be sent, on ice pack, Monday to Friday 800 to 2300 or Saturday, Sunday and Statutory Holidays 800 to 1600. Specimen must be received within 48 hours of collection.

CMV PCR PVPCR Urine, BAL,tissue, Sample to SPH Virology. Call Virology when sample arrives SPH Virology etc… (non blood) CMV NAT (non blood) CO2 TCO2 1 PST 0.5 mL lithium heparin plasma. Test within 24 hours. 24 Hour SPH Chem (HCO3, Carbon dioxide, bicarbonate) CO2, Fluid FCO2 1 PST, SST, RED 0.5 mL fluid. 24 Hour SPH Chem top or non FTYPE: PLEUR; DIA; PERIT; BAL; PCF FTYPE preservative Synovial fluid CO2 not available. container

Coagulation Screen (INR, PTT) COAGB 1 CIT Full tube required. PTT spun within 2 hours and tested within 4 24 Hour SPH Coag hours of collection. INR sample must be tested within 24 hours. If either test will not be tested within allowable time: centrifuge sample within 2 hrs (PTT) or 24 hours (INR), double spin plasma, freeze and ship on ice. Sample must arrive frozen in polypropylene tube Coagulation Inhibitors PTTS 3 CITRATE tubes Full citrate draw. Determine if patient is on oral anticoagulant. 24 Hour Y SPH Special Coag (Circulating Inhibitors, Inhibitor Specimen must be centrifuged within 2 hours of collection. Screen, PTT Mixing Studies) Minimum 3.0 mL plasma double spun, aliquot in polypropylene tube. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy of requisition for Special Coag.

Division of Pre and Post Examination, Page 74 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Cobalt NCODE 2 x 6 mL Royal Prior approval is mandatory. 10 days Y Inc. Blue K2 EDTA Centrifuge sample within 30 minutes. Decant into 2 x 3mL 57 Gervais Dr. (Special collection plasma into metal-free polypropylene vial. North York, ON M3C 1Z2 tubes) Additional information on HICL website: Phone 416-391-1499 Ext.248 ***Pre-order http://www.HICL.on.ca/search_tcna.asp?Tcstring=COB supply*** Fax Phone 416-385-1957 Send on ice pack (may be sent with other specimens on dry ice) . Copy of requisition for send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Cocaethylene COCAEB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Cocaine In Urine COCS Random urine >1.0 mL urine. Refrigerate if not done immediately. Order UDS Y – if DRSCB SPH (Outpatients) or UDP if screen ordered. Add DRSCB if Dr ordered (Benzoylecgonine) part of specifies Cocaine confirmation and give copy of requisition for UDS, or Medical Biochemist approval. UDP panel

Cocaine In Urine, Confirmation DRSCB Random urine Refrigerate. Send 50 mL Urine on ice pack. Y Provincial Toxicology Center Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Cocaine confirmation

Division of Pre and Post Examination, Page 75 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Coccidioides Serology COCCB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control No longer a TRAP test, no Med Micro approval required. 11/2014 C/O PHSA Laboratories – Lane Level Laboratory

Codeine CDNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Collect & Hold Group & Screen PXGRS 2 x 7ml EDTA Ward to call Transfusion Medicine to change order to Group & SPH Transfusion Medicine (Blood – Maternity Only (PHC) Screen (GRS) Bank)

Colorado Tick Fever Serology CTF 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Complement Fixation 5 mL SST GOLD Requisition must say which virus for complement fixation. SPH Virology Lab not available top tube

Complement mediated NCODE1 1 RED Top 2 mL of each serum and EDTA-plasma, each spun down and Y Prof. Dr. Michael Kirschfink Glomerulonephritis Ab tests: separated from the cells/clot, frozen, and sent on dry ice. Put Institute of Immunology NCODE2 1 Tall EDTA Fax Result to SPH Lab request sticker on requisition or on University of Heidelberg C3Neph, C3-Nephritis, anti- Sunset Printout. Im Neuenheimer Feld 305 CFH, anti-Factor H, and C5b-9 69120 Heidelberg, Germany tel: +49 6221 56 4076/4026 fax. +49 6221 56 5586 e-mail: [email protected] Minimum 1.0 mL collection using 3 mL EDTA. 0.25 mL for micro Complete Blood Count CBC 1 LAV 24 Hour SPH Hem EDTA. Sample stable 24 hrs at RT or 36 hrs at 4°C. Division of Pre and Post Examination, Page 76 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Complete Blood Count with CBCD 1 LAV Minimum 1.0 mL collection using 3 mL EDTA. 0.25 mL for micro 24 Hour SPH Hem Differential EDTA. Sample stable 24 hrs at RT or 36 hrs at 4°C.

Comprehensive Drug Analysis DRSCB 50 mL urine Refrigerate urine Y Provincial Toxicology Center (Urine)

Congenital Immunodeficiency IDEF 1 DK GRN Na Room temp. Copy of req to SPH immunology. Ensure CBCD is Mon – SPH Immunology add Heparin ordered. Fri CBCD 1 EDTA *ACD(A) also acceptable* Routine: Direct Antiglobulin Test DAT 1 LAV 3.0 mL EDTA blood. SPH Transfusion Medicine (Blood 1-3 hrs, (Coombs Test, Direct) Bank) STAT: 1 hour 24 hours

Copper CU 1 NAVY BLUE Centrifuge x2 Y Children’s Hospital Lab metal free 1st pour into navy vacutainer tube & centrifuge again add nd vacutainer tube 2 pour into: falcon polypropylene tubes and freeze 1.0 mL CPL minimum Serum ASAP. plus If performing Gadolinium-enhanced MRI, wait 2 days after 1 SST Gold top procedure for Gadolinium to clear before collecting sample.

Sendout person: Print CPL result with pending CU for Approval. Store at 4 °C and transport on ice. If not sending within the week, freeze and transport frozen on dry ice to Specimen Receiving 2J20. See Children’s trace elements collection: http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_201 40207_113746_Blood%20Collection%20for%20Trace%20Elemen ts%20rev%20Dec%202013.doc

Division of Pre and Post Examination, Page 77 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Copper (24 Hour Urine) CUU Collected with 15 Refrigerate. Send aliquot in metal free tube. Y Children’s Hospital Lab mL 6 mol/L HCL in Indicate if for trace element analysis or lead chelation test. Copy Add CRU, ACID WASHED and paste path to Intranet browser: UTIM container \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Miscellaneous Collection Procedures\Childrens_Hospital_24 HR URINE Collection Protocol March 9_09.pdf Copy and paste above path in Intranet browser. Do not need to verify for pH anymore as of April 03/09.

Copper, Free NCODE 1K2EDTA Dark Venipuncture must be performed using trace element collection Y Quest Diagnostics/ Nichols Institute blue top process (refer to Aluminum procedure: copy and paste to your 33608 Ortega Highway Intranet browser:: San Juan, Capistrano, *Special Collection \\vch\departments\Chemistry (Dept CA 92675 tube from PHCLAB)\Accessioning\Collection Booklet\Aluminum Collection Supervisor* 1(800) 553-5445 and Processing Procedure.doc

Obtain K2EDTA tube from Accessioning Supervisor’s Office. Quest will forward to: Patient MUST present with MSP preapproval letter prior to blood collection. Blood is only stable for 14 days. National Medical Services Lab 3701 Welsh Road, Willow Grove, Centrifuge K2EDTA and aliquot 3 mL (1.2 mL min.) plasma with PA 19090 MLA pipette and tips to Simport® Polystyrene aliquot tube. Send on ice pack. Copy of requisition for send out. Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker Test is performed every Thursday. Specimen must be received by Quest within 10 days of collection.

Copper, Tissue CUTIS Tissue See tissue trace elements. Children’s Hospital Send copy of requisition with transport batch.

Cortisone CORTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 78 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

if frozen must remain frozen until it is received at SPH

Cortisol, pm COPM 1 SST Gold top Cortisol collected between 14:00-18:00. Refrigerate Serum. 24 hour SPH Special Chemistry Stable for 4 days at 2 - 8 °C. Stable for 12 months at at -20 °C but

if frozen must remain frozen until it is received at SPH

Cortisol For Dexamethasone CODX 1 SST Gold top Collect after dexamethasone suppression. 0.5 mL serum. Mon-Fri SPH Special Chemistry Suppression Refrigerate serum. Stable for 4 days at 2 - 8 °C. Stable for 12

months at at -20 °C but if frozen must remain frozen until it is received at SPH Ask patient if dexamethasone was taken the night before, collect only if medication was taken. Consult with Biochemist if the patient did not take dexamethasone.

Cortisol, Free NCODE 1 SST Gold top 1 mL serum. Freeze serum. Copy of requisition for send out. Put Y Esoteric Laboratory Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Cortisol, Baseline CORF 1 SST Gold top 0.5 mL serum. Refrigerate serum. Stable for 4 days at 2 - 8 °C. 24 hour SPH Special Chemistry Stable for 12 months at at -20 °C but if frozen must remain frozen until it is received at SPH

Cortisol, Random CORT 1 SST Gold top 0.5 mL serum. Refrigerate serum. Stable for 4 days at 2 - 8 °C. 24 hour SPH Special Chemistry Stable for 12 months at at -20 °C but if frozen must remain frozen

until it is received at SPH

Cortisol, 30 minutes COR30 1 SST Gold top 0.5 mL serum. Refrigerate serum. Stable for 4 days at 2 - 8 °C. Mon-Fri SPH Special Chemistry Stable for 12 months at at -20 °C but if frozen must remain frozen

until it is received at SPH

Division of Pre and Post Examination, Page 79 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cortisol, 60 minutes COR60 1 SST Gold top 0.5 mL serum. Refrigerate serum. Stable for 4 days at 2 - 8 °C. Mon-Fri SPH Special Chemistry Stable for 12 months at at -20 °C but if frozen must remain frozen until it is received at SPH

Cortisol, Saliva SCORT Special Collection Special collection procedure is required; refer to the instructions VGH Kit in Outpatient with each kit. Please check Salivette® kit expiration date before Supplies cupboard issuing to patient; label kit with barcode and instruct patient to beside bring kit back to our lab ASAP. Stability: 1 week refrigerated. Accessioning Copy of requisition for patient to record collection time. Window Lab Accession: receive specimen in GenLab with actual collection time. Refrigerate and send to VH on Sunquest Transport batch on ice pack.

Cortisol, 24 hour Urine CORU 24 hr urine, no Refrigerate during collection. Measure 24 hour volume. Aliquot Weekly, SPH Special Chemistry preservative 3 mL in 12 X75 plastic tube for urine creatinine, 3 mL in 12 X 75 Wed. Add CRU, plastic tube for urine Cortisol from well mixed urine (do not send UTIM (CORR random whole collection). Centrifuge and decant both aliquots. Freeze urine not Cortisol tube, refrigerate creatinine and send on both on ice pack. orderable) Referring sites need to include 24 hour volume, patient’s height and weight on requisition.

Corticotropin Releasing NCODE 1 RED top 2-4 mL RED top serum. Send frozen on dry ice. Complete Y Quest Diagnostics/ Nichols Institute Hormone Nichols Institute requisition from quest binder. 33608 Ortega Highway San Juan, Capistrano, Mandatory MSP pre-approval required for Outpatients CA 92675 (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. 1(800) 553-5445 Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker

Cotinine SPQ 1 RED top or 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre >2 mL Urine Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; COTINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 80 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cotinine, urine DRSCB 10 mL urine Refrigerate and send on ice pack Y Provincial Toxicology Lab Sunquest LIS Order Entry instructions: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Cotinine Cotrimoxazole (Septra) SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: (Trimethoprim & TRIMEB SD0177=SER or ;Urine Sulfamethoxasole) TRISUL – SPQ1=;Cotrimoxazole N/A SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Coxiella Burnetii Serology QFEVB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Q fever; Q-fever) C/O PHSA Laboratories – Lane Level Laboratory

Coxiella Serology QFEVB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Coxiella Burnetii Serology) Level Laboratory

Creatine Test not available.

Creatinine CRE 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem

Division of Pre and Post Examination, Page 81 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 0.5 mL fluid. Synovial fluid CR not available. Creatinine – Fluid FCR 1 PST, SST, RED 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF top or non

preservative PPD Dialysate FTYPE codes (get PET info from Modifier field): container DIAL24 = Dialysate 24hr DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3 Creatinine, 24 hour urine CRU 24 hour urine no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem preservative (Acid hour collection (do not send whole collection). Referring sites Fri Add UTIM container need to include 24 hour volume, patient’s height and weight on acceptable) requisition. Refrigerate and send on ice pack.

Creatinine, random urine CRR Random urine 3 mL aliquot. Refrigerated. 24 Hour SPH Chem

Creatinine Clearance CRU 24 hour urine no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem UTIM preservative (Acid hour collection (do not send whole collection). Referring sites Fri container need to include 24 hour volume, patient’s height and weight on CRE acceptable) requisition. Refrigerate and send on ice pack. CCL 1 PST 0.5 mL lithium heparin plasma. Blood should be drawn during the 24 hours of urine collection but is acceptable to collect within the 24 hours before or after the urine collection. Routine: Cross Match GRS 2 Tall LAV SPH Transfusion Medicine (Blood 1-3 hrs, Bank) STAT: 1 hr 24 Hour

Creutzfeldt-Jakob Disease CJDB min 3 mL CSF fluid Freeze immediately. Forward via Microbiology to BCCDC. If SPH – Microbiology (CJD Disease) – Madcow Micro is unable to forward, then forward to BCCDC. Send in TDG BCCDC will forward to the National Disease with accordance to TC guidelines, or see appendices for Microbiology Lab at Health Canada additional information for specimen requirement and shipping in Winnipeg. (BCCDC Creutzfelt-Jakob information under CJD. Disease 14-3-3 Protein; TC Guidelines: Protein 14.3.3) http://www.tc.gc.ca/eng/tdg/clear-part2-339.htm#app3 Complete NML requisition: https://www.nml- lnm.gc.ca/guide2/infection_engview.php?refdiagID=14

Division of Pre and Post Examination, Page 82 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Pre-warm citrate tubes prior to collection. Collect and maintain all Cryofibrinogen CRYOFB 3 BLUE top citrate 6 days SPH Special Chem tubes@37 °C. and after 1 RED top (for sample CRYOG) Pre-warm STAT Lab Centrifuge; spin Citrate @37 °C immediately receipt to separate cells from plasma. Aliquot as follows: *all in warm (37 citrate tube 1 – aliquot in to tube and put “4 °C plasma” on label °C) water bath* citrate tube 2 – aliquot in to tube and put “4 °C plasma” on label citrate tube 3 – aliquot in to 2 tubes, put “4 °C plasma” on 1 and put “37 °C plasma” on second. Deliver to Special Chem, one in 37°C heat block and one in 4°C fridge.

Referring Lab: Label tubes “citrate plasma collected @37 °C” and “Red serum collected @37 °C”. Keep/Send plasma at room temp.

SPH Accessioning: follow CRYOG for RED top.

Preferred 8 hours fasting. Collect RED top @ 37 °C Cryoglobulin CRYOG 1 RED top in O 6 days SPH Special Chem 1. Allow Red top to clot @ 37 C for 60 minutes, set a Timer. (Pyroglobulin) warm ( 37 °C) after 2. Set the STAT Lab warm centrifuge to 37°C and spin 2 to 3 water bath sample full spins to reach temperature O receipt 3. After 60 minutes, Centrifuge @37 C 4. Label 2 tubes “Serum collected @37 °C” Aliquot serum to 2 tubes, ~1.0mL minimum serum in the 4°C tube. 5. Referring Labs: send at room temp.

SPH Accessioning: add Na azide to both tubes; deliver to Special Chem, one in 37°C heat block and one in 4°C fridge.

Do NOT let the samples sit for 24 hours. If it’s left on the warm block for an extended time, DO NOT spin the whole specimen! Referring Labs without a warm centrifuge: let specimen sit for 24 hours at 37 °C. Aliquot 2mL of serum from the clot retracted Red top and centrifuge the aliquoted serum. Centrifuge the serum. Then aliquot the spun serum to 2 tubes: label 37 °C and 4°C; minimum 1.0mL in each tube. Cryptococcal Antigen PCRAG 1 SST Gold top 0.5 mL serum. Refrigerate. 2 hours SPH – Microbiology 0.5 mL joint fluid. Anticoagulant containers such as lithium Crystal – Fluid FCRYSP 1 RED top or non Mon-Fri SPH Chem heparin, EDTA or oxalate may form crystals. (Fluid Crystal) preservative STAT available on Weekend dayshift. container

Division of Pre and Post Examination, Page 83 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

CSF Cell Count FCSF 0.5 mL CSF STAT. Refrigerate. Also order CDIFP STAT SPH Hematology

CSF Culture PCSFC 0.5 mL CSF STAT to Micro. 7 days SPH Microbiology

CSF Cytology CYTCSF 0.5 mL CSF STAT to Cytology on dayshift with copy of requisition if not SPH Cytology ordered in SCM. Refrigerate in Histology/Cytology bucket after hours (weekdays after 17:00 and weekends). STAT requests during Shift – page AP Tech.

CSF Glucose CSGL 0.5 mL CSF STAT. STAT SPH Chemistry

CSF LDH CSFLD 1.0 mL CSF Freeze. Send on ice pack VGH Lab

CSF Total Protein CSFTP 0.5 mL CSF STAT. STAT SPH Chem

CSF Virology PCSF 0.5 mL CSF Refrigerate. Copy of requisition for Virology 3 days SPH Virology

Cyanide CYANB whole blood Refrigerate blood; make sure tube is well sealed. Y Provincial Toxicology Center GREY top tube –

As Full As Possible

Cyclic Amp (Nephrogenous) Unavailable – May/07

Cyclic Amp (Urine) Unavailable – May/07

Cyclobenzaprine (Flexiril) CYCLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 84 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Trough level 10-14 hours for patient on twice/day dosing or 20-24 Cyclosporin Pre Level CSA 1 LAV Mon-Fri SPH Special Chem hours for patient on once/day dosing. Sample MUST have dose times, please followup with ward or patient or referring site if information does not arrive with sample. Sunquest Last Dose format: CSADLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 CSATLD: ;HH:MM e.g. ;13:50 Ask patient for dose time and must collect within 2 hr +/- 15 Cyclosporin 2 hour Post Level CSA2 1 LAV Mon-Fri SPH Special Chem minutes post dose. Sample MUST have dose times, please followup with ward or patient or referring site if information does not arrive with sample. Sunquest Last Dose format: CSADLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 CSATLD: ;HH:MM e.g. ;13:50 Cyproheptadine CYPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

Division of Pre and Post Examination, Page 85 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cysticercosis Serology CYSTIB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Taenia Solium Serology) Level Laboratory

Cystine in Urine CYSTIN 10 mL fresh early Daily SPH Chem (Qualitative BRAND test) morning urine preferred.

Cystine (Urine) RUAM 10 mL. Minimum Must mix urine with magnetic stir bar for 5 minutes Children’s Hospital – Biochemical Random Or Screen First morning void before removing aliquot for creatinine. Ship entire remaining Genetics Lab Add CRR preferred. specimen frozen, do not split with other testing. Room 2F22 604-875-2307

Cystine (Urine) Quantitative RUAM Whole 24-Hour No preservative. Freeze total collection. Children’s Hospital – Biochemical urine. Add each sample to bottle as voided. Genetics Lab Add CRU, First morning Room 2F22 UTIM specimen preferred 604-875-2307 over 24 HR urine.

Cystine, WBC (Blood) WCY 1 DRK GRN Na Refer patient to CW if possible, call Children’s if not possible at Y Children’s Hospital Lab (order Heparin tube 604-875-2307. WCT for Whole blood must arrive at C&W within 3 hrs of collection and known prior to 1200 hrs. Notify children’s hospital lab that the specimen Cytinosis is being sent. Send copy of requisition with transport batch. patient.)

Cystine, WBC(blood) – known WCT 1 DRK GRN Na Refer patient to Children’s Hospital Lab. If not possible, call Y Children’s Hospital Lab Cytinosis patients Heparin tube Children’s at 604-875-2307 or consult Supervisor. referred out Whole blood must arrive at C&W within 1 hrs of collection and prior to 1200 hrs. Notify children’s hospital lab that the specimen is being sent. Send copy of requisition with transport batch.

Cytomegalovirus (CMV ) Blood 3 mL EDTA After hour and referring Labs, aseptically separate within 4 hours Mon – SPH Virology Lab Culture – NOT AVAILABLE, plasma of collection into sterile plastic cryovial. Freeze at –20 OC and Fri order PCMVB instead. send Frozen. Copy of requisition for send out. Results in 3 days

Cytomegalovirus – IgG PCMVG 5 mL SST Gold top Refrigerate. 2x/week SPH Virology Lab SPH Virology code result in *Cord blood is 3 days unsuitable*

Division of Pre and Post Examination, Page 86 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cytomegalovirus – IgG CMVIGB 5mL SST Gold Top Refrigerate. Send on ice pack. BC Centre For Disease Control BCCDC code C/O PHSA Laboratories – Lane Level Laboratory

Cytomegalovirus – IgM Use 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control CMVSP

CMV DNA PCR for non-blood PVPCR 1 mL CSF, Urine, Freeze. Send on ice pack. Copy of requisition for Virology. Mon – SPH Virology samples BAL, other fluids or Fri Not routinely performed. tissue Results in 2 days

CYP11B1/2 DNA Chimeric NCODE 2 Tall EDTA LAV Minimum 6 mL EDTA whole blood. Specimen Stability: Y Athena Diagnostics Inc. Gene Fusion Test Room temperature/Refrigerated: 10 days. DO NOT Freeze. nd 200 Forest Street, 2 Floor (Monogenic Hypertension, Copy of requisition for send out. Patient must sign Bill73 Marlborough, MA 01752 USA Glucocorticoid-remediable Consent AND Athena genetic informed consent: Aldosteronism) 1 (800) 394-4493 https://lisdirect.questdiagnostics.com/Consent/static/consent- forms/4%20Athena%20Diagnostics/18%20Athena.pdf Sendout bench: Use Athena Diagnostic requisition: http://www.athenadiagnostics.com/getmedia/59803f78-3214-4fea- ab9f-f129ff2fcbe8/CanadaReq_Web_10-16-2015.pdf Attach copy of MSP pre-approval letter Test panel #779 CYP11B1/CYP11B2 Chimeric Gene Fusion Test Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Division of Pre and Post Examination, Page 87 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Cytotoxic Antibody Screen HLASCB 1 RED top Keep at room temperature. Date of collection and patient’s VGH Lab (Panel Reactive Antibody – birthdate must be on the tube. Immunology Tissue Typing (CASP PRA, DSA, Donor Specific not STAT requests (Kidney Transplant and Heart Transplant) must Antibody, HLA panel orderable) be sent immediately. Label TDG container with STAT stickers reactive antibody, HLA and send on Sunquest Transport Batch. antibody screening, Lymphocytotoxic antibodies) Transplant Unit Coordinator will notify VH Immunology Tech. (a.k.a at Transplant: Effective August 31st, 2015: The core lab technologist that lymphocyte or flow receives the notification is now responsible for the receipt, crossmatch) handling and shipping of all stat requests for cytotoxic antibody testing. The process is as follows: 1. Sample (1 red top) is collected by laboratory staff or 6B nurse as per normal protocols. 2. Blood collector hands the sample off to the unit coordinator or primary nurse. 3. Nurse or unit coordinator calls the core lab at local 63222 and informs the technologist that, “Stat cytotoxic antibodies are being tubed down now for patient _____ and must be sent to VGH stat”. 4. Nurse or unit coordinator tubes the samples to the lab with a yellow STAT flasher. 5. Responsible technologist personally performs or oversees the receipt, packaging and shipping of the sample to VGH stat. Inform the taxi driver to take the samples directly to VGH with no stops. 6. Responsible technologist calls VGH accessioning at 604- 875-4111 x61363 to inform them that the sample is in transit.

Notes: • Requests received for cytotoxic antibodies without the stat notification will be processed as routine. • The laboratory will no longer call the nursing unit to inquire. If laboratory staff are unsure of the urgency, they will consult with the core supervisor only. • If the core lab supervisor is not in the laboratory the designated technologist is responsible. • If the pneumatic tube is not operational, the nurse will bring the samples to the lab, ask for the core supervisor and deliver the samples to them personally.

Division of Pre and Post Examination, Page 88 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

D-Dimer Test DDIM 1 CIT Full citrate draw. 1.0 mL plasma from full tube collection. Spun 24 Hour SPH Coag. within 2 hours of collection and tested within 8 hours of collection. If test is not performed within 8 hours, double spin plasma and store at -20°C for up to 2 weeks, ship on ice. Specimen must arrive frozen in polypropylene tube. D-Xylose Tolerance Fasting DXY0 1 GOLD Xylose drink not available. Must be booked in Accessioning. Must Test unavailable as of May 8, have fasted 8 hours 2013 D-Xylose Tolerance 60 DXY60 1 GOLD Xylose drink not available. Must be booked in Accessioning. Must Minutes Test unavailable have fasted 8 hours as of May 8, 2013 D-Xylose Tolerance 120 DXY120 1 GOLD Xylose drink not available. Must be booked in Accessioning. Must Minutes Test unavailable have fasted 8 hours as of May 8, 2013 Dehydroepiandrosterone DHEAS 1 SST Gold top 0.5 mL serum in False Bottom or 13 x 75 Polypropylene tube. Mon & SPH Special Chemistry Sulphate Send with ice pack. Thurs

Delta Amino Levulinic Acid ALAU 24 hrs. Urine Specimen is to be collected in a dark bottle. No preservative. Y VGH Lab Refrigerate. Urine should be pH 4-7. It will only be done if porphyrins are elevated. Send urine creatinine result.

Dengue Serology DVS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (do not order DVMS or C/O PHSA Laboratories – Lane DVGS separately) Level Laboratory (Dengue Virus HI)

Deoxypyridinoline Crosslinks DEOX 1 mL well mixed Must collect random first morning specimen, refrigerate sample Lifelab Victoria via Lifelab Courier (Hydroxyproline –Urine) Urine and send on ice block or 24hr collection. Copy of requisition for 12 X 75 tube for send out. For additional information see 24hr urine collection. TEST NO LONGER DEOX Revised Sept/01 AVAILABLE Aug 2013 http://www.lifelabs.com/files/BC/BC_Physician_Newsletters/2013_ 5 mL Urine 12 X 75 August_Lab_Update.pdf C-telopeptide (HICL/Lifelab) tube for CRE Desipramine & Metabolite DESIPB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center (Norpramin Or Pertofrane) dosage, time of last dose, and other meds. For therapeutic drug

by HPLC monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Division of Pre and Post Examination, Page 89 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Dextromethorphan DEXTB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Diazepham (Valium) DZPNB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug

monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Dicyclomine Not available

Division of Pre and Post Examination, Page 90 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 0.5 mL serum. 12 hour post oral dose or 6 hour post IV – confirm Digoxin Level DIGO 24 Hour SPH Chem dose time with patient or nurse. Stable for 2 days at 2-8 °C. (Lanoxin) 1 SST Gold top Sunquest Last Dose format: DIGDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE DIGTLD: ;HH:MM e.g. ;13:50 or UNAVOE Diethylamine (Antabuse DIELB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center Metabolite, Disulfiram) requisition for send out.

Dihydropteridine Reductase DHPR NBS Card Blood dot card. Y Children’s Hospital Do NOT use the pneumatic tube system to transport wet bloodspot cards. Wet bloodspot cards must NOT be packaged in biohazard bags.

Division of Pre and Post Examination, Page 91 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Dihydrotestosterone NCODE 1 RED top 2 mL RED top serum (minimum 0.6 mL). Freeze serum and send Y Specimen Process Center on dry ice. Ensure Bill 73 is completed and copy of requisition Mayo Medical Laboratories (DHT (5 Alpha Reductase Profile for Sendout bench. 3050 Superior Drive NW blocked 5-a-dihydrotestosterone Rochester, MN 55901 for SPH) SPH Sendout: must generate Mayo Clinic Order in MayoLink, 5-Alpha- see Supervisor: 1-800-533-1710 Dihydrotestosterone https://orders.mayomedicallaboratories.com/en/login?SAMLart= Allodihydrotestosterone NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG Androstanolone) QTgyREYy%0A Mayo Test ID: DHTS Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Dilantin –Free FDIL 1 PST 0.5 mL lithium heparin plasma. Collect prior to next dose. VGH (free dilantin, free phenytoin. (FDILA Confirm dose time with patient. FDIL includes total and free.) addon to PHEN)

Diluted Thrombin Time, Direct NCODE 1 CIT Instructions: Full citrate draw. Specimen must be centrifuged Daily (M- Y SPH Special Coagulation Thrombin Inhibitors: within 2 hours of collection. 1.0 mL double spun plasma in F 07:00- Argatroban, Dabigatran polypropylene aliquot tube. Freeze at -20°C, refer in sample 15:00 must arrive at SPH frozen. Copy of requisition to Special only) Coagulation. Must indicate type of Direct Thrombin Inhibitors (anticoagulant). Diltiazem (Cardizem) DILTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 92 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Diphtheria Serology DIPB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Diphtheria Immune Status) C/O PHSA Laboratories – Lane (Diphtheria Antitoxin Level) Level Laboratory

Disopyramide (Norpace Or DISB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center Phythmodan) requisition for send out.

Division of Pre and Post Examination, Page 93 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Donath Landsteiner DLB 1 RED top at Keep at 37°C. Deliver immediately to accessioning. Y Children’s Hospital ° 37 C ° Allow to clot at 37 C for 30 minutes. Centrifuge and freeze Serum. Do not discard or freeze red cells. Send red cells and Serum together on ice pack. Send copy of requisition with transport batch. Double Stranded DNA only DSDNAS 1 SST Gold top 1 mL serum. Freeze. Send on ice pack. VGH Lab

Double Stranded DNA with ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab other autoimmune tests days @ 4°C. ordered

Down’s Syndrome CCGB 4 mL DRK GRN Key word “Trisomy” in eLab Cytogenetics Lab At Children’s Na Heparin Doctor’s name and date of collection must be included. Copy of Hospital via Children’s Lab Cytogenetic Test, Blood Cytogenetics requisition for send out with transport batch.

Doxepin & DXPNB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center Desmethylodoxepine dosage, time of last dose, and other meds. For therapeutic drug (Sinequan or Triadapin) monitoring specimens must be drawn at trough. Serum must be Tricyclic Antidepressants separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Doxepin Doxylamine DOXYB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center (Dicletin) dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

Division of Pre and Post Examination, Page 94 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Drug Screen, Qualitative CDSCR At least 20 mL. Refrigerate sample. Send on ice pack. Copy of requisition for Y VH Chem (Urine or Gastric Fluid) Urine OR (Gastric send out. Fluid 10-15 mL) Drugs of Abuse – Urine UDM 10 mL random Includes MTD, OPSC, BENZ, COCS, AMPHP AND THCSC 24 hour SPH Chem (Urine Drugs of Abuse for urine Battery no longer orderable as of May 6, 2015. Maternity) Drugs of Abuse – Urine UDS Random urine >1 mL urine. Includes MTD, OPSC, BENZ, COCS, AMPHP and 24 hour SPH Chem (Urine Drugs of Abuse for FENTU Outpatient) Drugs of Abuse – Urine UDP Random urine >1 mL urine. Includes OPSC, BENZ, COCS, AMPHP, MTD, 24 hour SPH Chem (Urine Drugs of Abuse for THCSC and FENTU Psychiatry) Add MTD to all UDP orders on inpatients.

Duloxetine SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; DULOXETINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 95 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

EBV IgG EBGSB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Epstein Barr Virus) C/O PHSA Laboratories – Lane (EBV Viral Capsid Level Laboratory Antibody IgG) EBV IgM Use 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Epstein Barr Virus) EBVSP C/O PHSA Laboratories – Lane (EBV Viral Capsid code Level Laboratory Antibody IgM) EBV IgA Test no longer available. PEBVA SPH Virology Lab (Epstein Barr Virus IgA) (Epstein-Barr – Viral Capsid Antibody IgA, EBV IgA Tumor Marker) EBV PCR PEBVB 6 mL EDTA Lav 3.0 mL EDTA plasma, minimum. After hours and referring Labs, Mon – SPH Virology Lab aseptically separate within 4 hours of collection into sterile plastic Fri (blood) cryovial. Results - EBV Viral Load ° in 2 days - EBV NAT (Nucleic Acid Freeze at –20 C. Send Frozen. Test) - Epstein Barr Virus PCR * Patients <19 years old are to CEBVL 1 RED top 2 mL RED top whole blood. Patients <19 years old are to be sent Children’s Hospital Virology be sent to BCCH.* to BCCH. Samples must be sent, on ice pack, Monday to Friday 800 to 2300 or Saturday, Sunday and Statutory Holidays 800 to 1600. Specimen must be received within 48 hours of collection.

EBV DNA PCR PVPCR 1 mL CSF or fluid Freeze. Send on ice pack Mon – SPH Virology Fri (non blood)

Echinococcosis Serology ECHNB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Eastern Equine Encephalitis EEE 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control Serology

Division of Pre and Post Examination, Page 96 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Eculizumab drug level NCODE 5 mL SST Gold top 2 mL serum. Allow blood to clot for 30 minutes at room Y Cambridge Biomedical Inc. temperature, centrifuge and separate serum into two 1.8mL 1320 Soldiers Field Road (serum Eculizumab) Cryovial tubes, freeze. Send on dry ice. Boston, MA 02135, USA Stability 12 months at -20 °C. 617-456-0700 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Ehrlichiosis NAT EHNAT 6 mL EDTA Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Anaplasma PCR, (or CSF, serum; Modify BCCDC’s Specimen Description with Med Micro initials if by Medical C/O PHSA Laboratories – Lane Ehrlichia PCR, Rickettsial specify sample Approved: E.g. SD0079=BLD-AMMMR Microbiologist Level Laboratory Infections) type)

Ehrlichiosis Serology EHRL 5 mL SST Gold top Refrigerate. Send on ice pack BCCDC ZEP (Anaplasma (BCCDC Sendout) phagocytophila Serology)

Elastase (Stool) ELASB At least 100 gram Freeze if testing is delayed more than 1 day immediately. Children’s Hospital of fresh stool. Lab Accessioning

Optimal 400G

Electrolytes, plasma (NA, K, LYTE 1 PST 0.5 mL lithium heparin plasma. Refrigerate. 24 Hour SPH Chem TCO2, CL) 0.5 mL fluid. Synovial fluid electrolytes not available. Electrolytes, Fluid FLYTE 1 PST, SST, RED 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF top or non FTYPE preservative PPD Dialysate FTYPE codes (get PET info from Modifier field): container DIAL24 = Dialysate 24hr DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3 Electrolytes+BUN+CRE REN 1 PST 0.5 mL lithium heparin plasma. Refrigerate 24 Hour SPH Chem

Electrolytes RNLP 1 PST 0.5 mL lithium heparin plasma. Refrigerate 24 Hour SPH Chem +BUN+CRE+GLUC

Electrolytes, 24 hour urine UE3U 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon-Fri SPH Chem preservative hour collection (do not send whole collection). Referring sites add CRU, need to include 24 hour volume, patient’s height and weight on UTIM requisition. Refrigerate and send on ice pack.

Division of Pre and Post Examination, Page 97 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Electrolytes, random urine UE3 Random urine 3 mL random urine. Refrigerate 24 Hour SPH Chem

Electrophoresis, serum PEL 1 SST Gold top 1.0 mL serum. Includes Total protein and albumin. Refrigerate. Mon – SPH Special Chem Fri

Electrophoresis, 24 hour urine UEPQ 24 hour urine, no Refrigerate during collection. 50 mL aliquot from well mixed 24 Mon – SPH Special Chem (Urine EP 24hr, Urine Bence preservative hour collection (do not send whole collection). Referring sites Fri Add CRU, Jones 24 hr; UBJ 24hr) (unsuitable in Acid) need to include 24 hour volume, patient’s height and weight on UTIM requisition. Refrigerate and send on ice pack.

Electrophoresis, random urine UEP Random urine 3 mL urine. Refrigerate and send on ice pack. Mon – SPH Special Chem Fri (Urine EP, random Bence Jones, BJP random)

Entamoeba Serology AMOEB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Enterovirus PCR PVPCR Non-blood only. Freeze CSF, refrigerate stool. Send on ice pack Mon – Y SPH Virology CSF, stool, swabs Fri (non blood)

Enterovirus RNA PCR

Enterovirus Serology EVIR DO NOT Serological testing for Enteroviruses (Polio, Coxsackie, Echo) is COLLECT not currently performed in BC. DO NOT ORDER. SAMPLE

Eosinophils, random urine EOSUP Random urine 10 mL urine unspun. Must arrive in the lab less than 4 hours after 24 Hour SPH Hem collection

Eosinophils, Stool Test not available 12/2017

Ephedrine EPHDB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 98 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Epstein-Barr-Virus or EBVSP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control EBV Serology – IgM And C/O PHSA Laboratories – Lane IgG Level Laboratory

Erythrocyte Sedimentation Rate ESRB 1 BLK top Diagnosis is required. Must be full collection tube. 24 Hour SPH Hem ESR with Diagnosis

Erythrocyte Sedimentation Rate ESRNP Do NOT collect sample. No barcodes generated. ESR without Diagnosis

Erythropoietin (EPO) Level EPOB 1 RED top 2 to 4 mL serum, minimum 2 mL separate within 30 minutes of N - if ordered STEM CELL LAB – TERRY FOX collection. Send frozen on Dry Ice. Copy of requisition for send by Drs.: th CBCD 675 West 10 Avenue out. Ezzat, Vancouver, BC, V5Z 1L3 Add CB4 Foltz Add CBCD if Outpatient or no recent CBC result. Append CB4 to to most S. Jackson, Do NOT send via BCCA or Lane most recent CBC result. recent C.Leger, Level Lab CB4: TERRY FOX STEM CELL ASSAY CBC H.Leitch, 604 675-8000 local 7746 BROOKE, CHERYL order Ross(Boldt), 604-675-8146 Ramadan, H. Put Fax Result to SPH Lab request sticker on requisition or on Sun, H. Sunset Printout. Merkley & P. Yenson

Division of Pre and Post Examination, Page 99 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Erythropoietin Antibodies NCODE 1 RED top Eprex or AMGEN. Testing site must provide FedEx Account for Follow information provided by Dr or (Anti-EPO antibody; Anti EPO shipping charges. patient.

Ab) Find out if patient is/has: 1). Part of VGH study protocol. Please see PRCA 855 study protocol in research binder or 2). Adverse event (PRCA, lack of efficacy, or decreased response) with Eprex treatment. Instruct ordering physician to contact Eprex coordinator, drug safety & surveillance at 1-800- 567-3331 or (416)-382-4824 to initiate antibody testing, and coordinate with laboratory accessioning supervisor to draw appropriate specimen, and send out. Specimen must not be drawn until patient is off Eprex treatment for at least 4 days. See Eprex tab for additional info. Maria Edwards Tube in Collection Follow instructions in kit provided by Dr’s office. Accessioning Manager, Lab Support kit. Study Program Supervisor to complete Janssen Research signed “Physician PPD Development EPO-IMU-001 Acknowledgement of Patient Consent” form and fax to number indicated. Call 416-382-5105 to inform fax was sent. 2244 Danby Road Richmond, VA 23230 USDA Statement and Commercial Invoice MUST be on SPH PHC USA Letterhead. Tel: 804-254-8430 Fax: 804-253-1104 Escitalopram ESCIB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Essential Fatty Acids EFA 1 Tall LAV Collect minimum 3 mL EDTA blood. For adults, fasting overnight Biochemical Genetics Lab (8 to 10 hours). For infants <1 year, 4 to 6 hours fast or collect Children’s Hospital before next feed (2 to 4 hours). Spin and Freeze plasma. Send frozen to BC Children’s Lab. Require MSP funding approval for testing. CH will forward to Kennedy Krieger Lab. Send copy of requisition with transport batch.

Estradiol E2 1 RED top 0.5 mL red top serum in False Bottom or 13 x 75 Polypropylene Mon & SPH Special Chem tube. Send on ice pack within 48 hrs of collection or freeze and Thurs send frozen.

Estriol NCODE 1 SST Gold top 1 mL serum. Only for pregnant patients. Method measures total LifeLabs Estrogen (Estriol Estrone and Estradiol). Copy of requisition for

send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Division of Pre and Post Examination, Page 100 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Ethchlorvynol (Placidyl) ETVYB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Ethyl Glucuronide EGLUCB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 101 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Ethylene Glycol EGLY 2 mL Lithium Refrigerate Serum. Write STAT on req & container. Send Osmolar Y VGH Lab – Phone To Notify That Heparin plasma Gap. Phone ward and request they contact on-call Medical Specimen Is Being Sent Stat Test – Process (Serum is Biochemist for approval. See instructions in methanol/ethylene Immediately 875 411 L Local 68203 acceptable) glycol section TIGHTLY STOPPERED.

Euglobin Lysis Not available

Extractable Nuclear Screen ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab (Extractable Nuclear Antigen) days @ 4°C.

Extractable Nuclear Antibodies ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab days @ 4°C.

Extractable Nuclear Antibodies, NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Other send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

Factor Assays II-XII, Factor II- F2 to 2 CIT Full draw Full citrate draw. Specimen must be centrifuged within 2 hours F9: 2-3 Y SPH Special Coag XII Levels F12 of collection. 1.5 mL double spun plasma per factor assay in 2 per (Factor II, Factor V, Factor VII, polypropylene aliquot tubes. Freeze at -20°C, refer in sample week. Factor IX, Factor X, Factor XI, must arrive at SPH frozen. Copy of requisition to Special other Factor XII Or F2, F5, F7, F9, Coagulation. Factors:1 F10, F11, F12) per week

Factor V Leiden TDNA 6 mL EDTA Refrigerate. Send whole blood on ice pack. Always perform with N - if ordered VGH Special Coag (Includes Testing For PTG – (do not open or Factor V Leiden. by Drs.: 875-4111 L66400 Prothrombin Gene Mutation) centrifuge) Ezzat, Foltz S. Jackson, C.Leger, H.Leitch, A. Rahmani Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Division of Pre and Post Examination, Page 102 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Factor VIII Coagulant F8COA 2 CIT Full draw Full citrate draw. Specimen must be centrifuged within 2 hours 2-3 times SPH Special Coag of collection. 1.5 mL double spun plasma in 2 aliquots per factor per week (Factor 8, F8, Factor 8 Activity, assay in polypropylene aliquot tube. Freeze at -20°C. Copy of Factor VIII activity, Factor 8 1 requisition to Special Coagulation. stage, Factor VIII 1 stage, Factor VIII one stage) Factor VIII Antigen VWB 2 CIT – Full draw Full citrate draw. Specimen must be centrifuged within 2 hours 1-2 times Y SPH Special Coag Von Willebrand Antigen of collection. 1.5 mL double spun citrate plasma in 2 per week (Factor) polypropylene aliquot tubes. Freeze at -20°C, refer in sample Ristocetin Co-Factor must arrive at SPH frozen. Copy of requisition to Special Von Willebrand Activity Coagulation. Also Collect 2 citrate tubes for F8AG.

Factor VIII Chromogenic Assay F8CHRO 3 CIT – Full draw Full citrate draw. Specimen must be centrifuged within 2 hours Mon – Y unless SPH Special Coag. (Factor 8 Chromogenic assay, of collection. 2.5 mL citrate plasma double spun, aliquot in 2 Fri ordered by Dr also order Chromogenic Factor VIII) polypropylene tubes. Freeze at -20°C, refer in sample must S. Jackson F8COA Dayshift arrive at SPH frozen. Copy of requisition for Special

Coagulation. Referring Labs must consult SPH Hematopathologist prior to sending sample. Factor VIII Inhibitor F8AB 1.5 mL plasma – F8IB orderable by Special Coag only. See Hematology prior to Once per Y SPH Special Coag (Factor 8 Antibody Screen, also order Full Cit draw collecting. week Factor VIII Bethesda Units) F8COA Full citrate draw. Specimen must be centrifuged within 2 hours unless of collection. 1.5 mL double spun citrate plasma in from polypropylene tube. Freeze at -20°C, refer in sample must arrive Children’s at SPH frozen. Copy of requisition to Special Coagulation. Hosp.

Factor XIII (F13) F13Q 2 CIT – Full draw Full citrate draw. Specimen must be centrifuged within 2 hours 1-2 times Y SPH Special Coag of collection. 1.5 mL double spun plasma per factor assay in 2 per week Factor XIII Antigen polypropylene aliquot tubes. Freeze at -20°C, refer in sample

must arrive at SPH frozen. Copy of requisition to Special Coagulation.

Fanconi Anemia CCGB 1 DARK GREEN Minimum 3 mL whole blood. Store and ship at room temperature. Children’s Hospital Cytogenetics Specimen must arrive at Cytogenetics Lab within 72 hours of Lab (breakage studies, Na Heparin collection. Copy of requisition for Sendout with transport batch. chromosome breakage studies, chromosome fragility testing, DEB testing, Mitomycin-C testing) Fasciola Serology FASC 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Division of Pre and Post Examination, Page 103 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fasting Blood Sugar FPG 1 PST 0.5 mL lithium heparin plasma. Must be fasting (prefer 9-12 hour) 24 Hour SPH Chem (FBS, Fasting glucose, glucose fasting) FE (Iron, Serum Iron and Total TRFE 1 PST Includes Iron, Transferrin, Transferrin Saturation, and Total Iron- 24 Hour SPH Chem Iron-Binding Capacity – Binding Capacity. TIBC, Iron profile) Fat in Urine (Chyluria) FATU Fresh random No longer available as of 12/2017 Daily SPH Chem urine – 20 mL Hospitals In-Common Laboratory Fecal Calprotectin NCODE Single Stool Must have MSP pre-approved letter 10 Days Inc. sample Freeze and send on dry ice. 57 Gervais Dr. North York, ON M3C 1Z2 http://www.hicl.on.ca/search_tcna.asp?TCString=CALP%20F

Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

Fecal-Leukocytes Feces Spec to Microbiology – indicate test and “only” or “also” Microbiology SPH

Fecal Fat (fat in feces) FFSP Random Stool Analysis should be done as soon as possible. Freeze specimen if Weekda SPH Chem delay in analysis. y

Fecal Fats FATGB 24h/48h/72h stool Provide BCBIO Fecal fat collection instruction to each patient: Y BC BIO For 24h, 48h Or 72h in pre weighed http://www.bcbio.com/pdfs/D0019672_FecalFat.pdf Quantitative paint can In collection procedure section Stool weight will be * freeze on Fridays. Put Fax Result to SPH Lab request sticker done by BC BIO. on requisition or on Sunset Printout.

Division of Pre and Post Examination, Page 104 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fecal Immunochemical Test – FITB (for 1 – Zip lock Kit 1 – Sample dropped off in blue see thru zip lock bag with req.. 1 – LifeLabs only 1 FIT kit required (not x3 ) LifeLabs distributed via Login FIT (Collection Date/Time is required on

kits only) LifeLabs requisition), barcode label specimen container and send to Update June 19, 2013 LifeLabs with copy of Requisition and Sunset req. 2 –BCCDC Until further notice, this test is 2 – Envelope Kit For Outpatient: NOT AVAILABLE to Inpatients. distributed via (GI Clinic is considered BCCDC  Login FIT separately from blood work with Collect time Outpatient.) Please direct “N” and copy of requisition for patient to return. requests to Accessioning  Open zip lock bag and barcode label specimen, supervisor or Medical Biochemist.  Instruct patient put time of collection on requisition..  Instruct patient to return specimen with req. To our Lab after collection within 4 days (Test is unsuitable if not testing within 7 days at LifeLabs). http://www.lifelabs.com/files/BC/patients/Stool_- _F.I.T._Fecal_Immunochemical_Test.pdf 2 – Sample comes in as envelope kit – forward to BCCDC without login.

Felodipine SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; FELODIPINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Fenoprofen (Nalfon) FEPRB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fentanyl, Urine FENTU Random urine >1.0 mL urine. Refrigerate if not done immediately. Order UDS 24 hours Y- if SPH Chemistry (Street names: Apache, China part of (Outpatients) or UDP if screen ordered. Add DRSCB if Dr confirmation white, Jackpot and Tango AKA UDS, or specifies Fentanyl confirmation (or aka names) and give copy ordered Duragesic) UDP of requisition for Medical Biochemist approval. panel Fentanyl, Urine confirmation DRSCB Random urine Refrigerate. Send on ice pack. Y Provincial Toxicology Center See Instructions Sunquest LIS Order Entry instructions: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Fentanyl

Ferritin FER 1 SST Gold top 0.5 mL serum (1.0 mL serum if lipemic) in False Bottom or 13 x 75 Mon – SPH Special Chemistry Polypropylene tube. Sample type: serum perferred. Fri *Plasma Acceptable (Li- Hep, Na-Hep, K2EDTA)*

Division of Pre and Post Examination, Page 106 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fetal Hemoglobulin HBEIB 3 mL EDTA Approval required by Hematopathologist. Copy of requisition for N - if ordered Children’s Hospital Lab approval. Send at room temperature on Sunquest transport by Drs.: batch. Ezzat, Foltz S. Jackson, C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson Routine: Fetal Bleed Screen (PHC Draw FBSPHC 1 Tall LAV Same as MI order. Deliver to Transfusion Medicine (blood bank) SPH Transfusion Medicine (Blood 1-3 hrs, Only, Maternal Investigation) with copy of requisition. Bank) STAT: 1 hr 24 hours

Fibrinogen QFIB 1 CIT 1.0 mL plasma from full tube collection. Plasma spun within 2 24 Hour SPH Coag hours of collection and tested within 8 hours of collection. If test is not performed within 8 hours, double spin plasma and store at -20°C for up to 2 weeks, Ship on ice. Specimen must arrive frozen in polypropylene tube Fibronectin Testing (Fetal FFN SWAB Send swab at room temperature. Clinical request form should be 24 Hour SPH Lab Fibronectin Testing) included.

Filariasis Antibodies (Serology) FILAB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

FISH –22 Micro Deletion CCGB 1 GREEN top Na See Children’s Hospital Handbook or Children’s Hospital Y Children’s Lab (Test Request Usually From Heparin send Cytogenetics Laboratory Test Directory Binder for more Cytogenetics Lab PACH Clinic – Dr. Marla whole blood at information. Copy of requisition for send out with transport batch. Keiss) room temperature.

Division of Pre and Post Examination, Page 107 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Store in fridge. Dose time required. Trough level 10-14 hours for FK506 TAC 3 mL EDTA Mon – SPH Special Chem patient on twice/day dosing or 20-24 hours for patient on once/day (Tacrolimus) Fri dosing. Sample MUST have dose times, please followup with (Prograf) ward or patient or referring site if information does not arrive with sample. Sunquest Last Dose format: TACDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 TACTLD: ;HH:MM e.g. ;13:50 Flecainide FLECB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 108 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fluconazole SPQ 1 Tall EDTA Centrifuge ASAP. 2 mL EDTA plasma. Freeze plasma. Serum Y BCCDC Prov Tox also acceptable. Send on ice pack. Ask for dose time. Approval Medical

from Microbiology is required. Pre and Post dose could be Microbi. requested. Pre dose is before the dosage. Post dose is 2-hour Approval Post oral or 30 minutes Post IV. Sunquest Order Entry instructions: Free Text in the following fields at Results: SD0177 = PLS-EDTA SPQ1 = ;Fluconazole SQDLD = ;DD MMM YYYY e.g. ;15 APR 2013 SQTLD = ;HH:MM e.g. ;07:00 Flucytosine NCODE 1 RED top Centrifuge ASAP. 2 mL RED top serum (minimum 0.6 mL). Y Specimen Process Center (5-Fluorocytosine) Freeze serum and send on dry ice. Ensure Bill 73 is completed Medical Mayo Medical Laboratories (Mayo code : FLUC) and copy of requisition for Sendout bench. Microbi. 3050 Superior Drive NW SPH Sendout: must generate Mayo Clinic Order in MayoLink, Approval Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Fluid CEA CEAB Fluid in sterile Refrigerate and send on ice pack. BC Centre For Disease Control (Carcinoembryonic Antigen) add container Sunquest Order Entry: – Lane Level Laboratory FLDTYP SPECFL: ;Fluid CEA

Fluid Cell Count FCCP Non preservative 0.5 mL fluid. 24 Hour SPH Chem FDIFP Container or EDTA FTYPE: PLEUR; SYNOV; DIA; PERIT; BAL; PCF

PPD Dialysate FTYPE codes (get PET info from Modifier field): DIAL24 = Dialysate 24hr DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3

Fluid Culture and Sensitivity PFLD Fluid in sterile SPH Microbiology (Fluid C&S) container Division of Pre and Post Examination, Page 109 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Fluid-Mucin Or Mucin Clot Synovial fluid – 5 Send on ice packs. Done at RCH Monday to Friday, 0800 to 1600 RCH (Also Known As Ropes Test) mL in 1 RED top, hours. no additive

*1 DRK GRN top lithium hep acceptable*

Fluorescence In Situ NCODE 1 DK GREEN Copy of requisition is mandatory. Destination will be based on VGH Lab Hybridization (FISH) Sodium Heparin the requisition provided. CH Lab BCCA Lab RCH Lab Hospitals In-Common Laboratory Fluoride FLRD 1 DKBLUE trace 2 mL serum or plasma in a falcon poly propylene tube. Separate Y Inc. metals tube or 1 within 2 hrs. Refrigerate Serum. Send on ice pack. Copy of 57 Gervais Dr. K2EDTA trace requisition for send out. Put Fax Result to SPH Lab request North York, ON M3C 1Z2 metals tube sticker on requisition or on Sunset Printout.

Additional information on HICL website: Phone 416-391-1499 Ext.248 http://www.HICL.on.ca/search_tcna.asp?tcstring=fructo Fax Phone 416-385-1957

Fluoxetine (Prozac) FLXTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. (includes Metabolite Norfluoxetine) DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Fluoxetine Flupenthixol (Fluanxol) FLUPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 110 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Flurazepam & FLURB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center Esalkyflurazepam dosage & time of last dose. Specimen should be collected prior to (Dalmane) next dose. Refrigerate. Send on ice pack.

Flurbiprofen FLUBB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 111 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Folate, Red Cell RCF if 3 mL EDTA whole Log in and file HCT result before sending to VGH. Y VGH Lab from lab blood Send on ice pack. physician

Folate , Serum FOL Test not billable. DO NOT COLLECT

Follicle Stimulating Hormone FSH 1 SST Gold top 0.5 mL serum in False Bottom or 13 x 75 Polypropylene tube. Mon & SPH Special Chemistry (Pituitary Gonadotropins +LH) Send on ice pack. Thurs

Francisella Tularensis Serology TUL 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Free Dilantin FDILA 1 mL plasma Refrigerate Serum, send on ice pack. VGH Lab (Serum acceptable)

Division of Pre and Post Examination, Page 112 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Free Fatty Acids (Total) FFAB 1 SST Gold top or For adults, fasting overnight (8 to 12 hours). For infants <1 year, 4 14 day Y Inc. to 6 hours fast or collect before next feed (2 to 4 hours).Separate 1 RED Top 57 Gervais Dr. and freeze immediately. Store and send frozen on dry ice. If the North York, ON M3C 1Z2 specimen thaws, it is unsuitable for analysis. Specimens

containing heparin are unsuitable for analysis – heparin plasma or Phone 416-391-1499 Ext.248 patients receiving heparin therapy are unsuitable for analysis. Stability is 14 days frozen. Fax Phone 416-385-1957 Copy of requisition for Sendout. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Free Hemoglobin FHGB 1 SST Gold top Spin at 3000rpm for 10 minutes. Remove serum and spin serum Mon – SPH Special Chemistry at 3000 rpm for 10 minutes. Minimum 1.0 mL double spun serum. Fri Refrigerate, stable for 1 week.

Free Light Chains KLS 1 SST Gold top 1 mL serum. Refrigerate. Tuesday SPH Special Chemistry Lab (Kappa, Lamda, Serum FLC)

Free PSA PSAFB 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control (see Prostatic Specific C/O PHSA Laboratories – Lane Antigen, Free – Includes Level Laboratory PSA Total, PSA Free, PSA Free Ratio)

Free T3 FT3 1 SST Gold top or 1.0 mL serum. Send on ice pack. Stable for 7 days at 2-8 °C. 24 Hour SPH Special Chemistry (free triiodothyronine) 1 RED top

Free T4 FT4 1 SST Gold top or 1.0 mL serum. Send on ice pack. Stable for 7 days at 2-8 °C. 24 Hour SPH Special Chemistry (free thyroxine) 1 RED top Hospitals In-Common Laboratory Fructosamine FRUTOB 1 RED top or 2 mL serum. Send frozen on dry ice. Copy of requisition for 5 days Y Inc. send out. 1 SST Gold top 57 Gervais Dr. Additional information on HICL website: North York, ON M3C 1Z2 http://www.HICL.on.ca/search_tcna.asp?tcstring=fructo Phone 416-391-1499 Ext.248 Put Fax Result to SPH Lab request sticker on requisition or on Fax Phone 416-385-1957 Sunset Printout.

FTI Do FT4

Furosemide FUROB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 113 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

G6PD Quantitative G6AB BABIES-ONE Prior approval required. Send cold on wet ice ASAP. Copy of 1 week Y Children’s Hospital (Glucose 6 Phosphate MICRO EDTA; requisition for send out. Dehydrogenase Assay) ADULTS-ONE Refer to Children’s eLab Handbook for more information. Do NOT order. Use G6PDP. 3mL EDTA tube http://www.elabhandbook.info/phsa/Default.aspx

Gabapentin (Neurontin) GABAB 1 RED top 2-4 mL RED top serum. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Indicate if for toxicity. Refrigerate Serum. Send on ice pack. If patient of Dr. Misri, already pre-approved.

GAD65, Blood GAD65B 5 mL SST Gold top Spin and separate immediately 2-4 mL serum. Send frozen on dry 30 days Y Specimen Control 4B2.10 WMC (minimum volume ice. Approval required for all patients. Copy of requisition for send (Glutamic Acid University of Alberta Hospital 1.0 mL serum) out. Stability 8 hours refrigerated, 3 months frozen. Decarboxylase Antibody 8440 – 112 Street Put Fax Result to SPH Lab request sticker on requisition or on Edmonton, Alberta Anti-Islet Cell Antibodies) Sunset Printout. T6G 2B7 780-407-7484

GAD65, CSF NCODE 1.0 mL CSF 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler send frozen on dry ice. Mitogen Advanced Diagnostics (Glutamic Acid Laboratory Decarboxylase Antibody) Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

Division of Pre and Post Examination, Page 114 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Galactocerebrosidase (Krabbe KRA 6.0 mL DARK Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room Y Children’s Hospital – Biochemical Enzyme, WBC) GREEN top temperature. Copy of requisition for Sendout. Collect Monday – Genetics Lab Lithium Heparin Thursday only or consult Supervisor. Room 2F22 Beta-Galactocerebrosidase, tube whole blood Galactocerebroside B- SPH Sendout person: 604-875-2307 galactosidase, Send specimen to Children’s Hospital Lab by 12:00 hrs Galactosylceramidase (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. Galactose Kinase (Increased GALKSB 6 mL EDTA whole Prior consultation is required. Do not spin. Keep at room Y Children’s Hospital – Biochemical Galactose Galactose-1- blood temp. Must deliver to BCCH within 3 hours of collection so blood Genetics Lab Phosphate In Blood) arrives prior to 1400 on Mon to Fri. Copy of requisition for send Room 2F22 out. NOT AVAILABLE 604-875-2307

Galactose-1-Phosphate G1P 3 mL PST Heparin No fasting required for adults. For infants, collect prior to next Y Children’s Hospital – Biochemical tube Whole blood feed. Transfer whole blood into two plastic tubes and freeze. Genetics Lab Transport on dry ice. Erroneous results after RBC transfusion. Room 2F22 Send copy of requisition with transport batch. 604-875-2307

Galactose-1-Phosphate Uridyl Test no longer available –revised SEP.26/11 Transferase

Gamma GT GGT 1 PST 0.5 mL lithium heparin. 24 Hour SPH Chem

Gastric pH and Occult Blood OBG Gastroccult Slides No longer available as of 12/2017 Mon – SPH – Urinalysis Fri

Gastrin GSTN 1 SST Gold top on Patient must be fasting minimum 8 hours. 0.5 mL serum collected BC Centre For Disease Control ice on ice and spun cold, freeze immediately (within 1 hour of FAST collection). Send frozen on dry ice, must arrive at BCCDC frozen. Affix pink “Gastrin and IL-2R” sticker to outer box.

Division of Pre and Post Examination, Page 115 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 1. Package using supplied FedEx Pak and complete pre GeneDx Cardiology Genetic RES2 2 tall EDTA GeneDx printed FedEx Waybill, bill to recipient FedEx Acc test Kit included in Kit #249659606. Complete Commercial Invoice. 207 Perry Parkway (may have a Blueprint kit with 2. Put specimens in Transport Batch, specify “SPH to Gaithersburg, MD 20877 only 1EDTA in kit, please GeneDx” in Batch. Put FedEx tracking number on Batch collect a second EDTA and add sheet. Store batch sheet in Sendout binder in GeneDx tab. (301)519-2100 it to kit) 3. Specimens stable for 7 days. Batch specimens and send www.genedx.com at Room Temp. within 3 days of collection. Do not send on Friday. 4. Batch and package maximum 3 Genedx kits in 1 FedEx Pak, or in larger TDG container if more than 3 GeneDx kits received; ship each Pak or container using 1 FedEx Waybill. Genotyping (Cystic Fibrosis) MDT 6 mL EDTA whole Testing available Mon to Friday 0800-1630 HR. Send samples at Children’s Hospital Molecular (Cystic Fibrosis Probes) blood. Do not room temperature. Diagnostic Laboratory Rm 2F40 (do not order CFTRCW) freeze. Specimen Send copy of requisition with transport batch. 875 – 2852 can be refrigerated Ordering Dr must complete Molecular Genetics Laboratory C&W if stored overnight requisition: or over the http://www.elabhandbook.info/phsa/Files/RequisitionForms%2f1 weekend. _20140722_042203_CWMG_REQ_0000_v4.2_General_Requis ition%20edit.pdf Must include on requisition whether confirmation of diagnosis or family history or carrier testing. Obtain last dose. Send aliquot on ice pack. Microsample should Gentamicin Pre Level GENPR 1.0 mL LITHIUM VGH Lab be in conical aliquot tube not adaptor cups. Send Pre and Post Heparin plasma dose sample if both are ordered; OR serum STAT orders and Babies: send STAT. Routine orders Friday-Sunday (all shifts): send within 2 hours of collection. Routine orders Monday-Thursday (15:00-07:00): aliquot and freeze. Leave note for day send-out tech.

Off-hour STAT: send via taxi STAT. Affix bright pink sticker for notification. Sunquest Last Dose format: GENDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE GENTLD: ;HH:MM e.g. ;13:50 or UNAVOE

Division of Pre and Post Examination, Page 116 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Obtain infusion Start and Stop time. Send aliquot on ice pack. Gentamicin Post Level GPO 1.0 mL LITHIUM VGH Lab Microsample should be in conical aliquot tube NOT adaptor cups. Heparin plasma Send Pre and Post dose sample if both are ordered. OR serum STAT orders and Babies: send STAT. Routine orders Friday-Sunday (all shifts): send within 2 hours of collection. Routine orders Monday-Thursday (15:00-07:00): aliquot and freeze. Leave note for dayshift send-out tech.

Off-hour STAT: send via Taxi STAT. Affix bright pink sticker for notification. Sunquest Infusion format: ITSG: ;HH:MM (Time Started) ITFG: ;HH:MM (Time Finished) Gentamicin Random Level GR 1.0 mL LITHIUM Obtain last dose information. Send aliquot on ice pack. VGH Lab Heparin plasma Microsample should be in conical aliquot tube NOT adaptor cups. OR serum STAT orders and Babies: send STAT. Routine orders Friday-Sunday (all shifts): send within 2 hours of collection. Routine orders Monday-Thursday (15:00-07:00): aliquot and freeze. Leave note for dayshift send-out tech.

Off-hour STAT: send via taxi STAT. Affix bright pink sticker for notification. Sunquest Last Dose format: GENDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE GENTLD: ;HH:MM e.g. ;13:50 or UNAVOE

GHB (Gamma GHBTB 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Hydroxybutyrate) Collect blood within 10 hours of ingestion. If greater than 10 hours, collect urine specimens. GHB usually undetectable in the urine by 12-24 hours.

Division of Pre and Post Examination, Page 117 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Gliclazide SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; GLICLAZIDE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Glucagon Stimulation done in Medical Short Stay to diagnose See test SPH Special Chem. HGH possible Hypopituitarism. CORT and HGH collected at Baseline, Glucagon Stimulation Test 1 GOLD CORT then after Glucagon given at 30 min intervals for 4 hours. HGH in False Bottom or 13 x 75 Polypropylene tube. Freeze, send on ice pack.

Glucagon Stimulation, Fasting GGSTF 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucagon Stimulation, 10 Min GGST10 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucagon Stimulation, 20 Min GGST20 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucose, Fasting FPG 1 PST 0.5 mL lithium heparin plasma. Fasting 9 – 12 hours. 24 Hour SPH Chem

Division of Pre and Post Examination, Page 118 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

24 Hour SPH Chem 0.5 mL fluid.

Sunquest Order Entry: 1 PST, SST, RED FTYPE: PLEUR; DIA; PERIT; BAL; PCF FGL top or non Glucose, Fluid PPD Dialysate FTYPE codes (get PET info from Modifier field): FTYPE preservative DIAL24 = Dialysate 24hr container DIALON = Dialysate Overnight DIALP1 = Dialysate PET 1 DIALP2 = Dialysate PET 2 DIALP3 = Dialysate PET 3 Glucose, Random GLUC 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucose, 1h PC 1HPC 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucose, 2h PC 2HPC 1 PST 0.5 mL lithium heparin plasma 24 Hour SPH Chem

Glucose, 24hr Urine GLUU 24hr Urine No preservative. 1 mL aliquot, must order urine creatinine. VGH Glucose Tolerance Gestational GTGSB 1 PST 0.5 mL lithium heparin plasma. Must obtain Due Date 24 Hour SPH Chem Screen 50g Glucose Tolerance Gestational GGTF 1 PST 0.5 mL lithium heparin plasma. Must be fasting 9 – 12 hours. 24 Hour SPH Chem 75g Fasting This tolerance test should NOT be preformed over the weekend. Glucose tolerance procedures should only be booked and performed during Monday to Friday between 0700 to approximately noon. Please check with the Professional staff if glucose tolerance is to be performed after 12 noon. Glucose Tolerance, Gest 75g GG1 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem 60 Min Glucose Tolerance, Gest 75g GG2 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem 120 Min Glucose Tolerance, Diabetic GT2DFB 1 PST 0.5 mL lithium heparin plasma. Must be fasting 9 – 12 hours. 24 Hour SPH Chem 75g Fasting This tolerance test should NOT be preformed over the weekend. Glucose tolerance procedures should only be booked and performed during Monday to Friday between 0700 to approximately noon. Please check with the Professional staff if glucose tolerance is to be performed after 12 noon. Glucose Tol, Diabetic 75g 120 GLT2 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min

Division of Pre and Post Examination, Page 119 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Glucose Tolerance GT5HFB 1 PST 0.5 mL lithium heparin plasma. Must be fasting 9 -12 hours. 24 Hour SPH Chem Hypoglycemic 75g Fasting This tolerance test should NOT be preformed over the weekend. Glucose tolerance procedures should only be booked and performed during Monday to Friday between 0700 to approximately noon. Please check with the Professional staff if glucose tolerance is to be performed after 12 noon. Glucose Tol, Hypog 75g 60 GT5H1 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min Glucose Tol, Hypog 75g 120 GT5H2 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min Glucose Tol, Hypog 75g 180 GT5H3 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min Glucose Tol, Hypog 75g 240 GT5H4 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min Glucose Tol, Hypog 75g 300 GT5H5 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem Min Glucose Tolerance Cystic GTCFFB 1 PST 0.5 mL lithium heparin plasma. Must be fasting 9 – 12 hours. 24 Hour SPH Chem Fibrosis Fasting This tolerance test should NOT be preformed over the weekend. Glucose tolerance procedures should only be booked and performed during Monday to Friday between 0700 to approximately noon. Please check with the Professional staff if glucose tolerance is to be performed after 12 noon.

Glucose Tol, CF 60 Min GTCF1 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem

Glucose Tol, CF 120 Min GTCF2 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem

1 PST Glu Pituitary Stim Test, 0 Min PSTFB 0.5 mL lithium heparin plasma and 1 mL serum. Includes FT4, See test SPH Chem 1 GOLD TSH, PRO, FSH, LH, CORT and GH

1 PST Glu Pituitary Stim Test, 30 Min PST30B 0.5 mL lithium heparin plasma and 1 mL serum. Order Battery See test SPH Chem 1 GOLD includes GH and CORT.

1 PST Glu Pituitary Stim Test, 60 Min PST60B 0.5 mL lithium heparin plasma and 1 mL serum. Order Battery See test SPH Chem 1 GOLD includes GH and CORT.

1 PST Glu Pituitary Stim Test, 90 Min PST90B 0.5 mL lithium heparin plasma and 1 mL serum. Order Battery See test SPH Chem 1 GOLD includes GH and CORT.

Glu Pituitary Stim Test, 120 1 PST PS120B 0.5 mL lithium heparin plasma and 1 mL serum. Order Battery See test SPH Chem Min 1 GOLD includes GH and CORT.

Division of Pre and Post Examination, Page 120 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Glutathione Peroxidase Test no longer available (Suspect Selenium Deficiency-Anti-Oxidant System Investigation) Glutathione Reductase Not available as of Oct01/05 Erythrocyte

Gluthethimide (Doriden) GLUTEB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Glycine Receptor antibodies NCODE 1 RED top or 1.0 mL serum aliquot, does not need to be sent frozen if it is Y Immunology Laboratory, Churchill clean and unhemolysed, and if it arrives in Oxford within two to Hospital, SST Gold top three days. Old Road, Headington Oxford Put on requisition: RESEARCH ONLY NO CHARGE. OX3 7LJ UK

Include copy of Dr’s email correspondence with Dr Angela (Fax +44 1865 225990 CSF Vincent in Oxford. Put Fax Result to SPH Lab request sticker Tel +44 1865 225995) on requisition or on Sunset Printout.

Division of Pre and Post Examination, Page 121 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Glycolic Acid NCODE 1 SST Gold top 2 mL serum. Send frozen on dry ice. Copy of requisition for send Y Dr. Albert Fraser out. Put Fax Result to SPH Lab request sticker on requisition Queen Elizabeth Health Sciences or on Sunset Printout. Center 1278 Towel Road, Halifax, Nova Scotia, B3h 2y9

Glycols NCODE 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab Hospitals In-Common Laboratory Gold (Serum) AU 1 NAVY BLUE Centrifuge x2 15 days Y st Inc. metal free 1 pour into navy vacutainer tube nd 57 Gervais Dr. vacutainer tube 2 pouring: into falcon polypropylene tubes North York, ON M3C 1Z2 (BD368380) 2 to 4 mL Serum (or BD368381 plasma). Copy of requisition for See TRACE sendout. Phone 416-391-1499 Ext.248 ELEMENTS in Transfer serum or plasma to polypropylene vial as Fax Phone 416-385-1957 collection soon as possible. Store and send cold. procedure section for additional information

Gold (Urine) Not Available Not available at Mayo Medical Lab – updated Apr/11

Gonorrhea Complement Test not available Fixation Test Or GCFT

Gonorrhoea-Serology Test not available

Growth Hormone HGH 1 SST Gold top Minimum volume is 140 uL serum in False Bottom or 13 x 75 Mon, SPH Special Chemistry Polypropylene tube. Send with ice pack. Tues, Wed, Fri SPH Sunquest LIS Order Entry: 1. Must use Order Comment field to indicate timing. 2. Must use Order Comment to indicate drug given E.g. Order Comment = 30 MIN POST or = 30 MIN POST ARGININE or = 30 MIN POST GLUCAGON ***If Growth Hormone series is requested and lacks necessary info, give copy of requisition to Special Chemistry***

Division of Pre and Post Examination, Page 122 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Growth Hormone Suppression GHSF 1 SST Gold top Minimum volume is 140 uL mL serum (for each timed sample) in Mon, SPH Special Chemistry (glucose tolerance drink given) GHS30 False Bottom or 13 x 75 Polypropylene tube. Send with ice Tues, GHS1 pack. Wed, Fri GHS90 Growth Hormone Suppression (75g glucose tolerance drink given GHS2 after collecting Fasting specimen):

GHSF – Fasting Growth Hormone Suppression includes glucose GHS30 -30 minutes Growth Hormone Suppression GHS1 -1 hour Growth Hormone Suppression GHS90 – 90 minutes Growth Hormone Suppression GHS2 – 2 hour Growth Hormone Suppression Copy of requisition for Special Chemistry Chem: Do not perform GLUC again for referred in samples.

Guaifensin SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Guaifensin SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

H. Pylori IgG Serology HELIB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (helicobactrr serology) Level Laboratory

Haloperidol (Haldol) HLPRB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage & time of last dose. Specimen should be collected prior to next dose. Refrigerate. Send on ice pack.

Division of Pre and Post Examination, Page 123 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hantavirus Nat HANTAR 6 mL EDTA Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Hantavirus PCR, Hanta (or SST Gold, by Medical Modify BCCDC’s Specimen Description with Med Micro initials if Virus PCR) FLUID, MISC, Microbiologist Approved: E.g. SD0176=BLD-AMMMR (Sin Nombre Virus, Hantaan Specify specimen Virus) type) -Arbovirus Infections

Hanta Virus Serology HANTA 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Haptoglobin HAP 1 SST Gold top 0.5 mL serum. Refrigerate. Mon – SPH Special Chem Fri

Hardisty Test (Platelet Factor 3) No longer available.

Heat Stability Test HSTBB 1 Tall EDTA tube Pre-approval by Hematopathologist is required. Test must be Y Children’s Hospital Unstable Hemoglobin Test pre-booked by contacting the BCCH Complex Hematology Laboratory. Sample should be less than 24 hours old when tested. Transport on cold pack, by overnight courier, to have specimen arrive at C&W lab by 12:00 hrs (noon) the day after collection. Do not freeze.

A normal control must be collected at the same time. 2 mL minimum volume if Hgb > 100 g/L; 4 mL minimum volume if Hgb < 100 g/L Heinz Body Stain HEIN 1 EDTA LAV 1.0 mL Whole blood. Mon – SPH Hem Fri

Helicobacter Pylori Breath Test Contact Lifelabs for specimen requirement Lifelabs

Division of Pre and Post Examination, Page 124 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Helicobacter Pylori Serology – HELIB 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control IgG C/O PHSA Laboratories – Lane Level Laboratory

Helicobacter Pylori Stool Ag HPSA Stool in sterile 5 grams of feces. Consultation with the BCCDC Program Head BC Centre For Disease Control container (604-707-2622) is required. Only done if previous serology done C/O PHSA Laboratories – Lane and reactive or patient was treated for HP. Level Laboratory Hematocrit Order 1 LAV 1 mL EDTA or 0.25 mL in micro-EDTA CBC

Hemochromatosis HFESP 1x 6 mL EDTA Do not freeze EDTA specimen, can be stored overnight or over Children’s Hospital Molecular (HFE Sibling/Parent Genetic TALL LAV the weekend at room temperature on the Sendout rack. Testing Diagnostic Laboratory Rm 2F40 Testing) available Mon to Friday 0800-1630HR. Send at room temp.. 875 – 2852 Use this code only if Copy of MGL or Outpatient requisition for send out. Requisition indicates SPH and MSJ Sendout Tech: Result HFESP test with HFECH Sibling/Parent is HFE code after forming Transport batch. Send to Children’s without C282Y/C282Y homozygote approval.

Hemochromatosis HFE Confirm HFEFER 1 x 6 mL EDTA Do not freeze EDTA specimen, can be stored overnight or over 3 weeks See Children’s Hospital Molecular Diagnosis TALL LAV the weekend at room temperature on the Sendout rack. Stability 1 “Hemochroma Diagnostic Laboratory Rm 2F40 week, testing available Mon to Friday 0800-1630HR. Send at tosis Send (Hemochromatosis Confirm) and 875 – 2852 room temperature. Out 5 mL SST Gold top Procedure” for Copy of MGL or Outpatient requisition for send out. PHFE MSJ: Send both EDTA and GOLD SST to SPH worksheet SPH Sendout Tech: refer to special worksheet procedure prior to instructions sending sample qualified for HFECD. Send on Transport Batch.

2mL Serum for Ferritin, Iron and Transferrin Saturation on Clink. SPH Chemistry

Hemoglobin Order 1 EDTA LAV 1.0 mL minimum draw in 3 mL EDTA or 0.25 mL in micro EDTA. 24 Hour SPH Hem CBC

Hemoglobin A1C HBA1C 1 EDTA LAV 3.0 mL whole blood. Mon – SPH Special Chem (Glycosylated Hemoglobin) Fri

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hemoglobin Electrophoresis HBEIB 3 mL EDTA Approval required by Hematopathologist. Copy of requisition for N - if ordered Children’s Hospital (Thalassemia screen, approval. Send at room temperature on Sunquest transport by Drs.: Add Thalassemia investigation, batch. Ezzat, CBCD, hemoglobinopathy Foltz AMOR investigation, Hemoglobin F, S. Jackson, Hemoglobin Investigation C.Leger, HPLC) H.Leitch, Ross(Boldt), Ramadan, H. Sun, H. Merkley & P. Yenson

Hemoglobin H Bodies HINCB 3 mL EDTA Approval required by Hematopathologist. Send at room Y Children’s Hospital temperature. Copy of requisition for send out.

Hgb S Fraction, Quantitative SHBS 3 mL EDTA Hemoglobin S (STAT) quantitative (for patients in sickle cell crisis) Mon – Y SPH Special Chemistry (STAT Quantitative Hgb S ordered by Hematology with Hematopathologist approval only. Fri Fraction) Routine Hemoglobin S or electrophoresis is sent to BCCH (code HBEIB for routine orders).

Hemophilia A or B Genotype NCODE 6 mL EDTA or 7 mL Only orderable via Hemophilia clinic. Request must come from Attention: Jayne Leggo ACD B one of Hematologists Drs. Ezzat, Jackson, Ross(Boldt), Leger, Department Of Pathology, Leitch, Hoeshi , P. Yenson, Hoeshi, H. Merkley and L. Sun. Must Richardson Laboratory, Room 201 provide Hemophilia A and B genotype testing requisition (see Queen’s University, Kingston, forms/reqs) must send on same day collection. If sample is Ontario, K7L 3N6 collected on Friday, freeze whole sample and send frozen on Tel: 613-533-3187 Monday. Copy of requisition for send out.

Hemosiderin, Urine HSID 10 mL fresh urine Approval required by Hematopathologist. Minimum 5 mL urine in Mon – Y SPH Hematology (can be refrigerated conical tube. Fri overnight) Hospitals In-Common Laboratory Heparin Challenge Test LLPREB 2 x 6 mL EDTA http://www.HICL.on.ca/search_tcna.asp?Tcstring=PHLA 10 days Inc. (Lipoprotein Lipase Pre – Dose Collect Pre and 30 m Post heparin stimulation (IV heparin 100 57 Gervais Dr. Lipases Plasma Post Heparin IU/kg body weight after a 14 h fast). Avoid hemolysis. Both North York, ON M3C 1Z2 Lipoprotein Lipase Pre & Post frozen and unfrozen specimens are required. Phone 416-391-1499 Ext.248 Pre: Send 6 mL unfrozen EDTA plasma. Fax Phone 416-385-1957

Division of Pre and Post Examination, Page 126 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Heparin Challenge Test LLPO 6 mL EDTA Post: (Draw from the arm opposite to the heparin injection arm) (Lipoprotein Lipase Post – Send: (Pre & Post) Dose) 1. 0.5 mL unfrozen EDTA plasma. 2. three 1.5 mL frozen aliquots of EDTA plasma. Label tubes identifying the Pre and Post specimens. Prior consultation is required before the analysis will be performed. Contact Client Care at (416) 422-3000 Ext. 300 or [email protected] Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Routine: Heparin Induced HITB 1 SST Gold 1.0 mL serum. Ward should complete “Request for Heparin- SPH Transfusion Medicine (Blood 1-3 hrs, Thrombocytopenia (HIT Gel Test Induced Thrombocytopenia (HIT) Assay” Bank) STAT: screen) replaced 1 hr by HITS Dec 4/13 24 hours Full tube collection required. All Orders must be accompanied by Heparin Induced HITS 1 CIT (2.7mL Na 24 hours SPH Hematology the “4T Score Worksheet” available from Hematology or: Thrombocytopenia (HIT) Citrate) \\vch\departments\Chemistry (Dept Routine: Antibodies PHCLAB)\Accessioning\Requisitions\T4_HIT_request_workshee 1-3 hrs. t.pdf STAT: Copy and paste above path in Intranet browser. HIT by ELISA from Referring 1hr Minimum 1.0 mL Platelet-poor citrated plasma: Hospitals 1. Spin whole blood. 2. Transfer the top three quarters of the plasma to an aliquot tube. 3. Spin and aliquot again and remove the top three quarters of the plasma to a polypropylene plastic tube suitable for freezing. Referring Labs must complete the “4Ts probability score” form and send with sample. Please send sample frozen on dry ice, specimen must arrive frozen. Copy of requisition for Hematology.

Division of Pre and Post Examination, Page 127 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HIT 1 SST Gold top 2 – 4 mL serum. HIT (by ELISA) is to be ordered only by SPH Hematology. If Routine request during off hours, sample should (by be aliquoted and frozen. ELISA) to

be ordered by Hem Tech ONLY

Hepatitis – Acute (PHBSAG, PAHEP 5 mL SST Gold top Refrigerate. SPH Virology Lab PHBSAB, PHAM, and PHCV) Hepatitis – Chronic or Previous PCHEP 5 mL SST Gold top Refrigerate SPH Virology Lab (PHBSAG, PHBSAB, PHBCAB, and PHCV) Hepatitis – Immune Status PIHEP 5 mL SST Gold top Refrigerate SPH Virology Lab (Includes PHBSAB and PHATOT) Hepatitis A IgM PHAM 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab Fri (Hepatitis A Serology, Anti Daily Hep A, Hepatitis A Acute)

Hepatitis A IgG Antibody (Total) PHAIG 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab Hepatitis A Immune Status Fri Hepatitis A IgG 2x /week Hep A IgG Results in 3 days Hep A vaccine Anti-HAV (Total) Anti HAV (Total)

Hepatitis B Surface Antigen PHBSAG 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab (Hep B Surface Ag, Hep B Fri Ag, Hep B carrier, Hep B Acute)

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hepatitis B Surface Antigen- NCODE 3 mL LAV EDTA After hours and referring Labs, aseptically separate plasma within Mon – SPH Virology Lab Quantitative 4 hours of collection into sterile plastic cryovial. Freeze at –20°C, Fri *enter send frozen. QHBSAG as test name

Hepatitis B Surface Antibody PHBSAB 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab (anti HBs; anti-HBs, Hep B Fri vaccine, hep B screen, Hep B immune status) Hepatitis B Core Total Antibody PHBCAB 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab (Hep Bc Ab) Fri

Hepatitis B Core IgM PHBCM 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab Fri 2x/week results in 3 days

Hepatitis Be Antigen PHBEAG 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab Fri 2x/week result in 3 days

Hepatitis Be Antibody PHBEAB 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab Fri 2x/week Results in 3 days

Hepatitis B DNA Viral Load HBDNAB 6 mL LAV EDTA 3.0 mL EDTA plasma, minimum. After hours and referring Labs, Mon – SPH Virology Lab - PCR aseptically separate within 4 hours of collection into sterile plastic Fri (Hep B DNA, Hep B PCR, HBV cryovial. Freeze at –20°C, send frozen. Results Viral Load) in 5 days

Hepatitis B Anti Viral HBRESB 6 mL LAV EDTA 3 mL EDTA plasma, minimum. After hours and referring Labs, 30 days SPH Virology Lab Resistance aseptically separate within 4 hours of collection into sterile plastic (Hepatitis B Resistance, cryovial. Freeze at –20°C, send frozen. HBV DNA Resistance, Hepatitis B Genotype, Hep B Genotype, HepB Genotype)

Division of Pre and Post Examination, Page 129 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hepatitis B RNA Call Dr to confirm test (Hep C RNA?) (No Such Test Code!!!)

Hepatitis C – Antibody PHCV 5 mL SST Gold top Refrigerate Mon – SPH Virology Lab (Hepatitis C, Hep C Ab) Fri Daily

Hepatitis C RNA or PCR HPCRBB 6 mL EDTA tube Do not spin or aliquot or open tube. Send whole specimen on ice BC Centre For Disease Control Qualitative pack. 3 mL Minimum required. C/O PHSA Laboratories – Lane Qualitative no longer Send STAT if request is for Cadaver Transplant Case or other Level Laboratory available, order Quantitative STAT request has been approved by BCCDC Medical Staff. Call BCCDC at (604)-707-2828 (or after hours at 604-707-2819) if approval status is not indicated on requisition.

Hepatitis C Viral Load or PCR HPCRBB 6 mL LAV EDTA Do not spin or aliquot or open tube. Send whole specimen on ice BC Centre For Disease Control Quantitative (NAT) pack. 3 mL minimum required. C/O PHSA Laboratories – Lane (Hep C Treatment Send STAT if request is for Cadaver Transplant Case or other Level Laboratory Monitoring, Hep C nucleic STAT request has been approved by BCCDC Medical Staff. acid testing) Call BCCDC at (604)-707-2828 (or after hours at 604-707-2819) if approval status is not indicated on requisition.

Special instructions required for Needlestick injury, BBF exposure, or Immunosuppression at Order Entry second screen: e.g. SPEC40 = PLS-;Needlestick injury workup

Hepatitis C Genotyping HEPCRB 6 mL LAV EDTA Do not spin or aliquot. Send whole specimen on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Innolipa Hepatitis C Level Laboratory Genotyping, HCV Genotype)

Hapatitis C Resistance Testing NCODE 6 mL LAV EDTA Whole blood. Do not spin or separate. Place specimen in CfE BC Centre of Excellence bucket in fridge. (NS3, NS5A)

Hepatitis D Antibody HDLTAB 5 mL SST Gold Refrigerate. BC Centre For Disease Control - Delta Antibody Or Serology Top tube C/O PHSA Laboratories – Lane (Hep D Ab) Level Laboratory

Division of Pre and Post Examination, Page 130 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hepatitis D Viral Load or PCR HDVPCR 6 mL LAV EDTA Centrifuge and separate EDTA plasma aseptically within 4 hours 20 days Y NML via SPH Virology (Hep D PCR, HEP D into a sterile cryovial and freeze. Send sample to Virology to be National Microbiology Lab Quantification PCR, Hepatitis sent out 1015 Arlington Street Delta viral Load by quantitative Fill National Microbiology Lab requisition and attach copy of Winnipeg, MB, R3E 3R2 PCR) original requisition. Put Fax Result to SPH Lab request sticker Tel: 204-789-6062 on requisition or on Sunset Printout. For more information: http://www.nml- lnm.gc.ca/guide2/infection_engview.php?Refdiagid=145

Hepatitis E Virus Serology HEVB 5 mL SST Gold top Refrigerate. BC Centre For Disease Control C/O PHSA Laboratories – Lane (anti Hep E Level Laboratory

Hereditary Hemorrhagic NCODE 2 X 6ML EDTA Collect only Mon – Wed, sample stability is only 48 hours; cost of Y Molecular Genetics Division Telangiectasia (HHT) testing is $3000.00. Send at room temperature. BEFORE The Hospital For Sick Children collecting blood, patient must provide: 555 University Avenue Room 3420, Hill Wing 1. MSP pre-approval letter prior to collection. Copy of letter to Toronto, On, M5G 1X8 Accessioning Technical Leader. Tel: 416-813-7200 2. Patient’s doctor must have completed the SickKids Molecular Genetics Requisition link below: http://www.sickkids.ca/pdfs/Paediatric%20Laboratory% 20Medicine/2269-MolecGeneticsReq_BillingForm.pdf 3. Out of province genetic referral request: http://www.genebc.ca/uploads/FORMS/CWMG_REQ_0210F _v3.0_%20Shipment_Request_Form.pdf

sickkids.ca SPH Sendout Tech: complete Sickkids requisition with Billing information – bill to SPH Lab Accessioning Technical Leader. HSV DNA PCR PVPCR 1 mL CSF Freeze. Send on ice pack Mon – SPH Virology (CSF Herpes Simplex PCR, Fri CSF HSV PCR)

Herpes Simplex Virus – IgG HSVIGB 5 mL SST Gold top Refrigerate. Do not order unless patient is pregnant and may BC Centre For Disease Control have had recent HSV exposure.

Division of Pre and Post Examination, Page 131 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HERPES SIMPLEX VIRUS – HEPRB 5 mL SST Gold Refrigerate. BC Centre For Disease Control IGG, IGM Top tube (HSV IgM is no longer performed, order HSVIGB per BCCDC)

HSV Type Specific Antibody HSVTSS 5 mL SST Gold top Refrigerate BC Centre For Disease Control EIA add HSVIGB

Herpes Simplex Virus Type 1 & PHSVTY 5 mL SST Gold top Freeze serum. Send on dry ice. Charge the patient for $130. Life Lab 2 Make 2 copies of payment receipt. Attach one copy to copy - Western Blot of requisition for Sendout, second copy with original requisition. See western blot binder for the process and LifeLab requisition. Refer to LifeLabs for updated test information: http://tests.lifelabs.com/test_information.aspx?id=35254&view=coll ection Complete Lifelab contract requisition. Put Fax Result to SPH Lab request sticker on requisition. Hexosaminidase, serum HEX 1 RED top on ice. If Tay Sachs carrier testing requested, have patient fill Children’s Hospital Supplemental information form for Ashkenazi Jewish Carrier and Tay Sachs (Non-Pregnant) Tay Sachs enzyme screening: Hexosaminidase,Serum http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_201 N-Acetyl-β- Glucosaminidase 40214_032456_CWMG_REQ_0110%20v2.2%20AJ%20Carrier% 20%26%20Tay%20Sachs%20Enzyme%20Screening%20Supp% 20Info%20Form.pdf Centrifuge and separate minimum 100 µL serum and freeze at -20°C. Ship frozen on dry ice.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) WEX is the WBC hexosaminidase and collected for pregnant Hexosaminidase, White blood WEX 2 X 6 mL Na Y Children’s Hospital cells Heparin or Li women or women on contraceptives and for a pregnant couple. (Confirm Tay Sachs or Heparin Minimum 3 mL whole blood. Keep at room temperature. Collect Sandhoff’s or Carrier Status and hand deliver to Sendout person before 08:30 Monday – if Pregnant, Total Friday or consult Supervisor before collection. Copy of requisition hexosaminidase, % A for Sendout. Hexosaminidase)) Patient must complete requisition “Supplemental Information Sheet, Ashkenazi Jewish Panel & Tay-Sachs Only Carrier Screening” and must be sent with the specimen (see eLab for form) SPH Sendout person:. Send specimen to Children’s without approval with 09:30 Dynamex Courier. Then give copy of requisition for approval with “Specimen already sent to CW” written on requisition. Specimens collected after 09:30: Seek approval before sending. Same day shipping by taxi, to arrive at Children’s Hospital Lab by 12:00 hrs, (no later than 13:00 Mon-Fri, DO NOT use CW Courier). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. Hgb Electrophoresis HBEIB 3 mL EDTA Approval required by Hematopathologist. Copy of requisition for N - if ordered Children’s Hospital approval. Send at room temperature on Sunquest transport by Drs.: batch. Ezzat, Foltz S. Jackson, C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Division of Pre and Post Examination, Page 133 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Hgb F (Fetal Hgb) HBEIB 3 mL EDTA Approval required by Hematopathologist. Copy of requisition for N - if ordered Children’s Hospital approval. Send at room temperature on Sunquest transport by Drs.: 875-2307 batch. Ezzat, Foltz S. Jackson, C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

High Density Lipoprotein HDLP 1 GOLD 1.0 mL serum. Fasting preferred. Refrigerate. Daily SPH Chem (HDL Cholesterol)

Histoplasmosis, Buffy Coat CBCD 1 EDTA LAV Not routinely available. Consult Hematopathologist Mon – SPH Hem Fri

HIV Antibody (Nominal) Adult HIVCA 5 mL SST Gold top Refrigerate. Mon – SPH Virology Lab HIV Confirmatory Test code is shared with BCCDC. Fri HIV P24 Antigen If Dr specify specimen to BCCDC, Order NCODE in Sunquest Lab Human Immunodeficiency and send a copy of the requisition to BCCDC. virus antibody AIDS Antibody HIV Ag/Ab Combo Anti HIV Ag/Ab Combo HIV serology HIV diagnosis HIV third/fourth generation HIV 1 and 2 Ag/Ab Combo

HIV Antibody (Non-Nominal) NCODE 5 mL SST Gold top Refrigerate. Must send a copy of the requisition for BCCDC. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

HIV Newborn/baby SPH Virology Lab Investigation VLB 3 mL EDTA (HIV investigation – newborn, HIV PCR)

Division of Pre and Post Examination, Page 134 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

All PHC babies/children Minimum 1.5 mL plasma (or as much as possible from 3 mL (no age limit) EDTA). After Hours**Aseptically separate plasma within 4 hrs of collection into sterile plastic cryovial. Freeze at –20°C Non PHC sites if PHC Medical Microbiologist approval is required. Y >1 year of age Minimum 1.5 mL plasma. After Hours**Aseptically separate plasma within 4 hrs of collection into sterile plastic cryovial. Freeze at –20°C Minimum 1.0 mL EDTA for Babies. Non PHC if NCODE 3 mL EDTA BCCDC **Do Not Open Tube And Do Not Spin ** Store in fridge. Send to <1 year of age BCCDC on ice pack with copy of the requisition to BCCDC. HIV Proviral DNA PCR HIVPB 6 mL EDTA tube Do not spin or aliquot. Send whole specimen on ice pack. BC Centre For Disease Control Not available: Order HIVTQ if patient is not Centre For Excellence C/O PHSA Laboratories – Lane Not Available Level Laboratory

HIV Viral Load VLB 6 mL EDTA Tall 3 mL EDTA plasma, minimum– after hours and referring Labs, Mon – SPH Virology Lab HIV 1 RNA Viral Load Lav aseptically separate within 4 hours of collection into sterile plastic Fri HIV-1 RNA PCR cryovial. Freeze at –20°C, send frozen. Result in HIV-1 RNA NAT 5 days Roche HIV-1 Taqman Assay, HIV quantitative NAT, Viral Load – HIV HIV 1 Quant NAT NCODE 6 mL EDTA for Do not spin or aliquot EDTA samples. Send whole specimen on BC Centre For Disease Control (HIV RNA NAT) Prenatal. ice pack. C/O PHSA Laboratories – Lane

Level Laboratory Use only for Prenatal Send STAT if request is for Cadaver Transplant Case or other STAT request has been approved by BCCDC Medical Staff. Use HIV Viral Load VLB code Call BCCDC at (604)-707-2828 (or after hours at 604-707-2819) all for others. if approval status is not indicated on requisition. Must send a copy of the requisition to BCCDC. Do not use HIVTQ BCCDC code until further notice.

HIV 2 PCR NCODE 6mL LAV EDTA 3 mL EDTA plasma, minimum– after hours and referring Labs, Y National Laboratory for HIV in aseptically separate within 4 hours of collection into sterile plastic Ottawa (HIV 2 Viral Load) cryovial. Freeze at –20°C, send frozen. Send to SPH Virology to be Send to SPH Virology to forward to Ottawa forwarded to Ottawa

Division of Pre and Post Examination, Page 135 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HIV Pooled NAAT, RNA NAAT NCODE 5 mL SST Gold top HIV Pooled RNA Nucleic Acid Amplification Test (NAAT) is BC Centre For Disease Control entered in Sunquest by BCCDC. C/O PHSA Laboratories – Lane HIV NAAT Level Laboratory Must send a copy of the requisition to BCCDC.

HIV Viral Load, CSF PCR PVPCR 1.0 mL CSF BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

HIV Genotyping or Phenotype NCODE Collect one 6 mL 3 mL EDTA plasma, store and send frozen BC Centre For Excellence EDTA tube for VLB (HIV Drug Resistance Testing, Add VLB and HIV Genotype SPH Accessioning: ARV Resistance Testing, Anti to order VLB by SPH Virology RetroViral Resistance Testing, Before 14:30: Refrigerate EDTA in Virology rack. Put HIV Protease-RT, Integrase, gP41, Genotype Requisition in Centre for Excellence (CFE) FREEZER plasma V3) bucket after writing on the requisition “Sample sent to virology” and placing it in a biohazard bag. (CFE will retrieve Virology’s Viral Load sample once VLB is completed). Samples received from other sites cold or at room temperature – write on requisition and tube “Recd Cold/RT Mmm DD HH:mm” and refrigerate EDTA in Virology rack.

After 14:30: 1. Spin blood, aliquot plasma, and freeze in Virology freezer rack. 2. Put HIV Genotype Requisition in the Centre for Excellence (CFE) FREEZER bucket after writing on the requisition “Sample sent to virology” and placing it in a biohazard bag. If frozen plasma sample received along with the HIV Viral load sample, send all the frozen plasma tubes to Virology along with the requisition. 3. Samples received from other sites cold or at room temperature – write on requisition and tube “Recd Cold/RT Mmm DD HH:mm” and freeze in Virology rack. Note: All Centre for Excellence requisitions go in the Centre For Excellence (CFE) FREEZER bucket. No HIV Genotype plasma samples should be in the Centre For Excellence (CFE) FREEZER bucket. Result NCODE1: REFTO1=;CFE RECD1=HIDE

Division of Pre and Post Examination, Page 136 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HIV Tropism (CFE patients: NCODE 3 mL EDTA Tube Freeze whole blood EDTA, store and send frozen. BC Centre For Excellence Proviral DNA V3 Genotyping, SPH Accessioning: HIV CR5) Freeze whole blood EDTA tube in Centre for Excellence (CFE) freezer bucket along with the requisition. Result NCODE1: REFTO1=;CFE RECD1=HIDE HIV Western Blot NCODE 5 mL SST Gold top Refrigerate. Must include copy of requisition for BCCDC. BC Centre For Disease Control Order C/O PHSA Laboratories – Lane HIVWEB Level Laboratory if instructed by SPH Virology

Histoplasma Antigen Test, HISTAG Urine Send Serum with urine on ice pack. Send SST Serum with sample Y –TRAP BC Centre For Disease Control Urine (also need serum) (Specify sample type; refer to Smarterm or BCCDC collection). by Medical Will forward to Miravista Lab, Microbiologist Indianapolis. Modify BCCDC’s Specimen Description with Med Micro initials if Approved: E.g. SD0061=URN-AMMMR

Histoplasma Antigen Test, HISTAG 5 mL SST Gold top Send Serum on ice pack. Y-TRAP BC Centre For Disease Control Serum Sunquest Order Entry: by Medical Will forward to Miravista Lab, Microbiologist . Remove M container and Add GS container at Container Indianapolis. and Specimen Entry screen (Routing Screen).

. Specify sample is blood and modify BCCDC’s Specimen Description with Med Micro initials if Approved: E.g.: SD0061=BLD-AMMMR (search for other MM initials in SQ)

Histoplasmosis Serology HISTB 5 mL SST Gold top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control Modify BCCDC’s Specimen Description with Med Micro initials if by Medical C/O PHSA Laboratories – Lane (Histoplasma Serology) Approved: E.g. SD0169=BLD-AMMMR (search for other MM Microbiologist Level Laboratory initials in SQ)

HLA Typing – HLATYB Class I (ABC) – Keep specimen at room temperature. VGH Lab 1 ACD YELLOW Date & time of collection must be on the specimens & on the Tissue Typing top requisition 910 W. 10th Class II (DR) – 5 ACD YELLOW top

Division of Pre and Post Examination, Page 137 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HLA A29 NCODE 1 ACD YELLOW HLA typing for Birdshot retinopathy (should have query this Mon – VGH Lab diagnosis). Keep at room temperature. Fri Tissue Typing

HLA B1502 for Carbamazepine NCODE 1 ACD YELLOW or 4 mL Whole blood. Keep at room temperature. Date & time of VGH Lab sensitivity 1 Tall EDTA LAV collection must be on the specimens & on the requisition or print Tissue Typing. Sunset requisition. Both solutions A and B acceptable. HLA B27 Antigen – Disease B27 1 ACD YELLOW Keep specimen at room temperature. VGH Lab Association top Date & time of collection must be on the specimens & on the Tissue Typing requisition. 910 W. 10th Both solutions A A clinical diagnosis must accompany req. Revised Aug/03 and B acceptable.

HLA B51 NCODE 1 ACD (YELLOW Keep specimen at room temperature. VGH Lab top) Date & time of collection must be on the specimens & on the Tissue Typing requisition. 910 W. 10th Both solutions A A clinical diagnosis must accompany req. Updated Feb.09/11 and B acceptable.

HLA B5701 NCODE 3 mL EDTA Tube Freeze whole blood EDTA, store and send frozen. BC Centre For Excellence QA Manager, Carolyn Beatty, (Abacavir sensitivity test) SPH Accessioning: SPH Local 69145 Freeze whole blood EDTA tube in Centre for Excellence (CFE) freezer bucket along with the requisition. Result NCODE1: REFTO1=;CFE RECD1=HIDE HLA B5801 NCODE 1 ACD (YELLOW) Indicate “Query allopurinol sensitivity” as diagnosis on VGH Immunology Lab requisition. Send at room temperature, specimen viable for 48 Both solutions A hours only. 6 mL EDTA tube also acceptable. and B acceptable.

HLA DR2 & HLA DR3 NCODE 1 ACD (YELLOW) Indicate testing is for Narcolepsy or Celiac Disease on VGH Immunology Lab HLA typing for Narcolepsy requisition. Send at room temperature, specimen viable for 48 Both solutions A gene, HLA-DR, HLA-DR hours only. 6 mL EDTA tube also acceptable. and B acceptable. Phenotyping, narcolepsy HLA DNA panel

HLA Typing For Bone Marrow HLABM 2 YELLOW (ACD Keep specimen at room temperature. VGH Lab ‘B’) Date & time of collection must be on the specimens & on the Tissue Typing requisition. 910 W. 10th A clinical diagnosis must accompany req.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HLA Typing from Heart HLAPRE 1 RED top and 2 Keep specimen at room temperature. VGH Lab Transplants ACD ‘B’ Yellow top Date & time of collection must be on the specimens & on the Tissue Typing th requisition. 910 W. 10 A clinical diagnosis must accompany req.

Homocysteine HCYS 1 EDTA LAV on ice Collect on ice. Separate within 1 hour of collection. Freeze. Send VGH Lab on ice pack

Homocysteine Free RANDOM urine Urine-first morning specimen Y Children’s Hospital Freeze immediately upon collection and keep frozen. Biochemical Genetics Lab (Confirm With Ward That They + Actually Want A Free And Not Blood-fasting sample collected on ice. Must spin immediately & Ph#875-2307 Local 7436 A Total Homocysteine.) ONE Heparin tube do pff immediately. Freeze & send on dry ice. Consultation with Free Homocysteine not available (GREEN) biochemist recommended. at BCCH – Updated Feb01/05 (Preweighed Tubes Of Sulfosalicylic KL. ON ICE Pff = remove plasma. Add 50 mg sulfosalicylic acid per mL of Acid Are In The Send Out Supplies plasma vortex. Centrifuge at –10°C. Freeze and send on dry ice. Drawer.) Supernatant – label as pff

Homovanillic Acid HVAU 24 HR urine Collect in collection bottle containing 15mL of 6N HCL. If not VGH Lab 15 mL 6M HCL collected in acid, acidify in lab, to pH 2 – 4, only if entire collection CRU preservative is received. Acidification must be performed within 12 hours UTIM after completion of 24 hr urine collection. Aliquot 50 mL of a well- mixed 24hr collection, refrigerate, send on ice pack.

Homovanillic Acid HVAR 20 mL random Must have prior approval. Y VGH Lab urine Random is performed only on children. Acidify in lab to pH 2 – 4. Acidification must be performed within 36 hours of random urine collection. Minimum 12 mL refrigerate, send on ice pack HPL Not available

HPRT (Hypoxanthine-Guanine 1 Heparin tube 1 heparin tube and also tube collected from a normal patient to be Y University Of Michigan Medical Phophoribosyltransferase) (GREEN top) used as a control. Check also biochemical genetics Center APRT, and PRPP Synthetase Alberta children’s hospital. Approval required for all patients. Attn: Dr. Thomas D.Palella Levels Dept Of Internal Medicine, Div. Of Rheumatology, 5520, Msrb I, 1150 W. Medical Center Dr. , Ann Arbor, Michigan 48109 (Ph (313)747-3413

Division of Pre and Post Examination, Page 139 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

HSV (Herpes Simplex Virus, HSVIGB 5 mL SST Gold top Refrigerate. BC Centre For Disease Control HSV IgG, HSV screen) C/O PHSA Laboratories – Lane Level Laboratory

HSV (Herpes Simplex Virus) – 5 mL SST Gold top Refrigerate. BC Centre For Disease Control IgM C/O PHSA Laboratories – Lane Level Laboratory Test no longer performed HSV 1 & 2 Antibody by PHSVTY 1 SST Gold top 2 mL serum. Freeze serum. Send on dry ice. Charge the patient Lifelab Victoria Western Blot for $130. Make 2 copies of payment receipt. Attach one via Local Lifelab copy to copy of requisition for Sendout, second copy with original requisition. SPH Sendout person: complete Lifelabs special requisition: \\vch\departments\Chemistry%20(Dept%20PHCLAB)\Accessionin g\Requisitions\A1984%20SPH%20HSVT%20Reg.pdf Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. HSV NAT or PCR non blood PVPCR CSF, fluid, swabs SPH Virology

HTLV I/II Antibody or Serology HTLVB 5 mL SST Gold top Refrigerate. BC Centre For Disease Control C/O PHSA Laboratories – Lane - Human T Lymphotropic Virus *See instructions if If specimen is CSF (must have serum collected and sent): must Level Laboratory Type I/II CSF ordered* change specimen type at Order Entry: Container and Specimen Entry window – Remove GS container and add CC container. HTLV I/II PCR Testing HTLVPB 6 mL LAV EDTA Do not spin or aliquot. Send whole specimen at room temperature BC Centre For Disease Control on Monday or Tuesday only; specimen must arrive at National Lab C/O PHSA Laboratories – Lane **Contact BCCDC HIV Lab for HIV Reference Services within 48 hours of collection. Copy of Level Laboratory prior to collection at Original and NLHRS requisition for BCCDC to send to Winnipeg. BCCDC will send to: (604) 660-9709** NLHRS requisition: (copy and paste path to Intranet browser) National Laboratory for HIV **Collect only on Monday and Reference Services (NLHRS) \\vch\departments\Chemistry (Dept Tuesday** Dr. John Kim PHCLAB)\Accessioning\Requisitions\NLHRS PCR VL Req HIV National Microbiology Laboratory **Send out person-send out HTLV Testing Rev B July 23 2014.pdf 1015 Arlington Street, Room J1139 immediately after contacting Put Fax Result to SPH Lab request sticker on requisition or on Winnipeg, MB, R3E 3R2 BCCDC** Sunset Printout. (204) 789-6522 / (204) 789-6523

Division of Pre and Post Examination, Page 140 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Human Chorionic Gonadotropin HCG 1 PST Light green 1.0 mL lithium heparin plasma or serum. Refrigerate, send on ice 24 Hour SPH Chem. (pregnancy test, HCG Total, top pack. Stable for 3 days at 2-8 °C. HCG Quantitative) *1 SST Gold top or DO NOT ORDER ON MALES RED top –use order code HCGTMB acceptable*

Human Chorionic Gonadotropin HCGTMB 1 SST Gold top 1 mL serum. Freeze. Send on ice pack. BC Centre For Disease Control (HCG Tumor Marker, Beta C/O PHSA Laboratories – Lane Human Chorionic Level Laboratory Gonadotropin)

Human Herpes Virus 6 HHV6GB 5 mL SST Gold top Spin and refrigerate. Must have SPH Medical Microbiologist Y Children’s Hospital Lab Serology code consult with CW Microbiologist, test will only be performed with O r RED top (HHV6 IgG, HHV6 serology) blocked at instruction from CW Microbiologist. Must send a copy of the Order requisition with the transport batch. Entry. Children’s Hospital Lab Human Herpes Virus 6 PCR NCODE 6 mL LAV EDTA Do not spin or aliquot. Send whole specimen on ice pack. Must Y (HHV6 PCR, HHV6 NAT) have SPH Medical Microbiologist consult with CW Microbiologist, test will only be performed with instruction from CW Microbiologist. Must send a copy of the requisition with the transport batch.

Human Herpes Virus 8 DNA HHV8P 6 mL LAV EDTA 3mL EDTA plasma, minimum. After hours and referring Labs, Weekly SPH Virology Lab PCR aseptically separate within 4 hours of collection into sterile plastic (HHV8 DNA PCR) cryovial. Freeze at –20°C. Send frozen.

Human Papilloma Virus Not available

Human Placental Lactogen Not available

Hunter Enzyme, Serum HUNS 1 RED top 2-4 mL RED top serum. Prior approval is Required. Freeze Y Children’s Hospital – Biochemical (Iduronate Sulphatase, Serum) serum. Send frozen on dry ice. Send copy of requisition with Genetics Lab transport batch. Room 2F22 604-875-2307

Hydrochlorothiazide HDCLTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center (Hydrodiuril) requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Hydrochlorothiazide Hydrocodone HDCODB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Hydrocodone 1 RED top Hydrocortisone HDCTB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Hydrocortisone Hydromorphone, serum HYDMB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center (Dilaudid )

Hydromorphone, urine DRSCB 2 mL urine Refrigerate, send on ice pack Y Provincial Toxicology Center (Confirmation) Sunquest Order Entry: Remove RED default tube at Route screen and add UR container type.

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Hydroxy-Butyric- Not available, Dehydrogenase suggest LDH ISO 1 RED top Hydroxychloroquine HYDCHB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Hydroxyxhloroquine 1 RED top Hydroxyzine HYDXB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Hydroxyzine 1 RED top Ibuprofen IBUPB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center (Motrin Or Advil) requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Ibuprofen Immunoglobulin A (IgA) IGA 1 SST Gold top 0.5 mL serum. Refrigerate. Mon – SPH Special Chem Fri

Division of Pre and Post Examination, Page 143 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

IgD, Immunoglobulin D IGD 1 SST Gold top 1 mL serum. Refrigerate. No approval on patient diagnosed with Y VGH Lab IgD

IgE IGE 1 SST Gold top 1.0 mL serum in a False Bottom or 13 x 75 Polypropylene tube. Mon, SPH Special Chem No visible hemolysis. Freeze. Tues, Wed, Fri

IgG (Immunoglobulin G) IGGP 1 SST Gold top 0.5 mL serum. Refrigerate. Mon – SPH Special Chem Fri 1 RED top IgG Blocking Antibody NCODE 1.5 mL serum. Refrigerate. Copy of requisition for send out. Put Y BCBIO Lab (Blocking-Antibodies) Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

IgG CSF IGGCA 1 mL CSF Freeze. Send frozen on ice pack Y VGH Lab

IgG Subclasses IGGSB 1 SST Gold top Thursda SPH Special Chem Lab 1 mL. Serum total. Freeze and store in freezer in IGGSUB rack (IGGS1, IGGS2, IGGS3, y (refrigerated serum is stable for 8 days). IGGS4, IGG1, IGG2, IGG3,

IGG4)

IgM, Immunoglobulin M IGM 1 SST Gold top Mon – SPH Special Chem Lab 0.5 mL serum. Refrigerate Fri

Imipramine & Metabolites IMPB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center by HPLC dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Immune Cell Markers IMMCM 2 DRK GRN Na Copy of requisition to immunology. Store at room temp. If Mon – Y SPH – Immunology (Immunophenotyping, Cell add Heparin tube. collection is on Friday, consult Immunology tech. Test available Fri Markers, Flow Cytometry, CBCD 1 EDTA for CBC & Mon-Fri. B+T Cells, B And T Cell Autodiff Order IDEF if B & T cell ordered along with Immunoglobulins or Subsets, T+ B Cell) *ACD(A) also diagnosis is “Recurring infection”. acceptable*

Immunofixation IF Do NOT order. Special Chemistry will review PEL or UEP result Weekly Y SPH Special Chem (Ordered by Special Chem and order IF or UIF. UIF Tech only) Copy of requisition for Special Chemistry.

Immunoglobulin D IGD 1 SST Gold top 1 mL serum. Refrigerate. No approval on patient diagnosed with Y VGH Lab IgD

Immunoglobulins (includes: IMMUNO 1 SST Gold top 0.5 mL serum. Refrigerate. Mon – SPH Special Chem IGA, IGM, IGGP) Fri (Ig profile, immunoglobulin profile)

Immunoreactive Trypsinogen IRTDOT 2-3 blood dots on A copy of the original req with any doctor comments must be send Y Children’s Hospital Whatman 903 NBS together when requiring approval. Copy of requisition for send card (Newborn out. Screening card, Do NOT use the pneumatic tube system to transport wet Blood Dot Card) bloodspot cards. Wet bloodspot cards must NOT be packaged or in biohazard bags. Collect 3mL EDTA Allow to air dry blood dots. Fill patient information on NBS card and then apply and send NBS card at room temp and send serum frozen. blood from EDTA to NBS card.

Indomethacin (Indocin) INMETB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Division of Pre and Post Examination, Page 146 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Infliximab & Anti-Infliximab IFXB 1 RED top or Minimum volume: 1.0 mL of serum. Centrifuge and make 2 2 weeks Y Specimen Control 4B2.10 WMC Antibodies aliquots (0.5 mL each). Freeze aliquots and send on ice pack or from day University of Alberta Hospital ® 1 SST Gold top Requires (anti-INFα drug, Remicade ) dry ice (preferred). Stability: 30 days frozen, 7 days refrigerated of receipt SPH 8440 – 112 Street or at ambient temperature. at UAH. Infliximab Edmonton, Alberta Test available to Outpatients Sunquest Order Entry last screen: Last dose is REQUIRED Order Form T6G 2B7 and Referred-In locations IFXDLD= ;DD MMM YYYY e.g. 03 NOV 2014 completed by only, Inpatients must have IFXTLD=HIDE (Do NOT remove or change this) 780-407-7484 GI. Biochemist approval.

Other Health Authorities: send frozen sample to SPH Lab *specimen must be accompanied by Accessioning with completed SPH Infliximab Order form and Exception: 1. SPH Infliximab Order Form Routine requisition. specimens 2. UAH Third party requisition. from MSP billable – outpatient, non-responders only, trough is Children’s considered <2 weeks prior to next infusion Hospital do Anti-infliximab antibody test is reflexively performed based on not require the infliximab concentration. approval; STAT SPH Lab Instructions: requests – Save all remaining serum in 2 mL eppindorf tube (labeled with see reprinted barcode), store in -70°C Research freezer second Supervisorm shelf “Infliximab Extras” box. ust be sent the same Sendout Person: day. 1. Batch samples and send every Thursday. 2. Send SPH Order form 3. Send completed UAH Third party Req (attach barcode containing PHN or manually write). (copy and paste path in Intranet browser) \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Requisitions\Third PartyInflximab (FEB 2015).pdf

4. Do NOT send Sunset printout.

SPH Order Form: \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Requisitions\PHC-LA203 Infliximab Test Requisition (22Oct2014).pdf

Division of Pre and Post Examination, Page 147 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Influenza A & B Viruses 5 mL SST Gold Test Not available. SPH Virology Lab Top tube

Influenza A/B RNA PCR RESPCR 2 mL Refrigerate. Send on ice pack. Sample must be received by Mon – SPH Virology Nasopharyngeal virology by noon. Fri Wash or NP swab, tracheal aspirate or BAL

Influenza-Blood 5 mL SST Gold Test not available SPH Virology Lab Top tube Hospitals In-Common Laboratory Inhibin A NCODE 1 SST Gold top 2 – 4 mL Serum. Prior approval is required. Copy of requisition 6 days Y Inc. for send out. 57 Gervais Dr. Not useful if ordered alone. And should not be interpreted as a North York, ON M3C 1Z2 prenatal screening test. Phone 416-391-1499 Ext.248 Put Fax Result to SPH Lab request sticker on requisition or on Fax Phone 416-385-1957 Sunset Printout.

Inhibin B NCODE 1 RED top 2 mL serum, minimum 0.2 mL. Freeze serum and send on dry ice. Y Specimen Process Center Prior approval is required. Ensure Bill 73 is completed and Mayo Medical Laboratories Mayo Test ID: INHB *1SST Gold top copy of requisition for Sendout bench. 3050 Superior Drive NW acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Test for infertility in males and females. Not included as part of standard clinical practice. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Division of Pre and Post Examination, Page 148 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Inhibin (A & B) NCODE 1 RED top 2 mL serum, minimum 0.6mL. Prior approval is required. Ensure Y Specimen Process Center Bill 73 is completed and copy of requisition for Sendout bench. Mayo Medical Laboratories - Tumour Marker *1 SSTGold top 3050 Superior Drive NW acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, Mayo Test ID: INHAB see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Tumor marker useful for diagnosis/screening for aluminium cell tumor in females and for monitoring sex cord stromal tumors in males. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Innolipa Hepatitis C Genotyping HEPCRB 6 mL EDTA tube Do not spin or aliquot. Send whole specimen on ice pack. Copy BC Centre For Disease Control of requisition for send out. C/O PHSA Laboratories – Lane Level Laboratory

INR INRB 1 CIT Full tube required. INR sample must be tested within 24 hours. If SPH Coag (Prothrombin Time, PT) INR will not be tested within 24 hours: centrifuge sample within 24 hours, double spin plasma, freeze and ship on ice. Sample must arrive frozen in polypropylene tube.

Insulin INS 1 SST Gold top Minimum 8 hr fast preferred. Minimum volume 120 uL serum in Mon, SPH Special Chemistry or tube, freeze within 1 FAST False Bottom 13 x 75 Polypropylene Tues, hour of collection and send with dry ice, must arrive at SPH Wed, Fri frozen. Please consult with Special Chem supervisor if these specimen types are collected: Lithium heparin, K3EDTA, and Sodium Citrate. Non-fasting requests +/- Glucose result from other labs: (phone referring lab if fasting status is not indicated on requisition and ask for status and Glucose result) Reminder: a FPG result is FASTING!  If glucose result NOT available → order INS, do NOT give copy of requisition to Special Chem.  If glucose result available, record Glucose on requisition → order INS and give copy of requisition to Special Chemistry.

Division of Pre and Post Examination, Page 149 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Insulin Series HVSTMB 5 mL SST Gold top Freeze serum in False Bottom or 13 x 75 Polypropylene tube Mon – SPH Special Chemistry (Ca Stimulated HV Insulin) and send with dry ice, must arrive at SPH frozen. Fri Copy of requisition for Special Chemistry Sunquest LIS Order Entry: free text Sample Time in Modifier field and result STIMS (stimulation site) and STIMT (Sample Time) in second screen. Hospitals In-Common Laboratory Insulin Antibodies NCODE 1 RED top or 2 – 4 mL serum, aliquot and freeze ASAP. Send Serum frozen on 15 days Y Inc. dry ice. Copy of requisition for send out. Put Fax Result to SPH 1 SST Gold top 57 Gervais Dr. Lab request sticker on requisition or on Sunset Printout. North York, ON M3C 1Z2 Additional information on HICL website: http://www.HICL.on.ca/search_tcna.asp?tcstring=insab Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 Insulin-Like Growth Factor IGF1 5 mL SST Gold top 0.5 mL serum Freeze serum (or plasma) ASAP and send on dry Mon – SPH Special Chemistry (Somatomedin-C) ice. Specimens that arrive at 2-8C are acceptable if they were Fri shipped frozen that day.

Insulin-Like Growth Factor II NCODE 1 RED top 0.5 mL red top serum, aliquot and freeze within 1 hour of Y Specimen Process Center (IGF-II, IGF2, IGF 2, IGF-2, collection. Send frozen on dry ice. Ensure Bill 73 is completed Mayo Medical Laboratories (CW code Somatomedian A) and copy of requisition for Sendout bench. 3050 Superior Drive NW GF2 Rochester, MN 55901 Mayo Test ID: FIGF2 blocked SPH Sendout: must generate Mayo Clinic Order in MayoLink, 1-800-533-1710 for SPH) see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= Mayo will forward to: NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG Esoterix Endocrinology QTgyREYy%0A 4301 Lost Hills Road Mandatory MSP pre-approval required for Outpatients Calabasas Hills, CA 91301 (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Insulin-Like Growth Factor BP3B 1 RED top or 2 – 4 mL serum or Lith Heparin plasma, aliquot and freeze within 14 days Y University of Alberta Hospital, Binding Protein 3 (IGFBP3) 3 hours of collection. Send Serum frozen on dry ice. Copy of SST Gold top or Dept. of Lab Medicine &Pathology, requisition for send out. Put Fax Result to SPH Lab request Walter MacKenzie Centre, PST Light green sticker on requisition or on Sunset Printout. Specimen Control, Core Lab, Room top Additional information on AHS website: 4B210 http://www4.albertahealthservices.ca/labservices/index.asp?id=1 8440-112 Street 029&tests=I&zoneid=1&details=true Edmonton, AB T6G 2B7 CANADA 780-407-7484

Division of Pre and Post Examination, Page 150 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Insulin Receptor Antibodies NCODE 10 mL serum Freeze specimen and send on dry ice. Approval required for all Y Dr.J.Olefsky patients. Copy of requisition for send out. University Of Colorado Information outdated, Health Sciences Cener supervisor must validate prior to Mandatory MSP pre-approval required for Outpatients th B151-4200 E.9 Ave. collection. (request to be done by patient Dr.). For Outpatients, do not Denver collect without MSP preapproval if test is ordered alone. Colorado 80262 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Interferon, Beta Neutralizing INTFB 2 SST Gold top 5 mL serum (minimum 1 mL) Patient must be MS positive. UBC Research – Dr Oger’s Lab Antibody Reference lab needs to know which interferon drug patient is on. http://www.ubcneurology.com/NI%20Lab/NI%20Services.html Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Joanne Luider INF-g Pathway NCODE 2 DRK GRN top Collect and ship by FedEx on Monday. Whole blood at room Y Calgary Laboratory Services, Flow Na hep temperature. Sample must arrive in Calgary on Tuesday. (CD119 protein expression, Cytometry INF-gR1, pSTAT1 signalling) Complete Calgary Lab Services Out of Province requisition. McCaig Tower Rm 7509, Foothills Medical Centre Put Fax Result to SPH Lab request sticker on requisition or on th 1403-29 St. N.W. Sunset Printout. Calgary, Alberta, Canada T2N 2T9 Contact Performing Lab when test is picked up by FedEx. (403) 944-8995 Interferon Gamma, Antibodies NCODE 5 DRK GRN top Prior to blood collection: Y NIH/NIAID/LCID Na hep (INF gamma antibodies, anti- 1. Ordering Dr must have prior consultation with SPH Attn: Dr. Steve Holland INFγ, Abs to INF gamma, INF Biochemist and NIH Research Lab Dr. Steve Holland, 9000 Rockville Pike gamma Abs) branch chief. Bldg 10 CRC B3-4209 Bethesda, MD 20892 2. Obtain Genetics Consent form from: Joie Davis,Pediatric 301 443 5806 Nurse Practitioner, Genetics NIH/NIAID phone: 828 595 2486 3. Patient must sign Research Consent form from NIH and Bill73 for consent to send specimens to the USA. Store and send whole blood at room temperature on day of collection or next day with priority next day delivery service.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Interleukin 1a NCODE 1 RED top 2 to 4 mL serum. Minimum 2 mL serum or plasma separate and Y Specimen Process Center (IL-1, IL1, IL1a, Interleukin 1 freeze immediately in plastic vial. Patient should NOT be on any Mayo Medical Laboratories *6mL SST Gold top alpha) Corticosteroids, anti-inflammatory medications or pain killers, if 3050 Superior Drive NW or 6 mL LAV top possible, for at least 48 hours prior to collection of specimen. Rochester, MN 55901 Mayo Test ID: FINTA acceptable* Stability 365 days frozen, send on dry ice. Ensure Bill 73 is 1-800-533-1710 completed and copy of requisition for Sendout bench. MML forward to Interscience SPH Sendout: must generate Mayo Clinic Order in MayoLink, Institute see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Interleukin 1b NCODE 1 RED top or 3.0 mL serum or plasma separate and freeze immediately in Y Specimen Process Center (IL1 beta, IL-1, IL1, IL1b) plastic vial. Patient should NOT be on any Corticosteroids, anti- Mayo Medical Laboratories 6 mL LAV top inflammatory medications or pain killers, if possible, for at least 3050 Superior Drive NW Mayo Test ID: FINTB 48 hours prior to collection of specimen. Stability 365 days Rochester, MN 55901 frozen, send on dry ice. Ensure Bill 73 is completed and copy 1-800-533-1710 of requisition for Sendout bench. MML forward to Interscience SPH Sendout: must generate Mayo Clinic Order in MayoLink, Institute see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Division of Pre and Post Examination, Page 152 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Interleukin 2 (IL2, IL-2) NCODE 1 RED top 1 mL serum frozen in plastic vial. Stability 365 days frozen, send Y Specimen Process Center on dry ice. Ensure Bill 73 is completed and copy of requisition Mayo Medical Laboratories Mayo Test ID: FIL2M for Sendout bench. 3050 Superior Drive NW *1 SST Gold top or Rochester, MN 55901 1 DRK GRN top Li SPH Sendout: must generate Mayo Clinic Order in MayoLink, 1-800-533-1710 hep acceptable* see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= MML forward to ARUP NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Interleukin 2 Receptor (sCD25), IL2R 5 mL SST Gold top 1 mL serum; 0.3 mL minimum. Freeze serum and send on dry ice. BC Centre For Disease Control Soluble Affix pink “Gastrin and IL-2R” sticker to outer box.

(IL2 Receptor, IL2R, sIL2R)

Interleukin-4 (IL-4, IL 4) NCODE 1 RED top 1 mL serum frozen in plastic vial. Stability 28 days frozen. Y Specimen Process Center Ensure Bill 73 is completed and copy of requisition for Sendout Mayo Medical Laboratories Mayo Test ID: FIL4S *1 SST Gold top bench. 3050 Superior Drive NW acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= MML forwards to Viracor-IBT Lab NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Interleukin-5 (IL-5, IL5) NCODE 1 LAV top 1 mL plasma frozen in plastic vial. Ensure Bill 73 is completed Y Specimen Process Center and copy of requisition for Sendout bench. Mayo Medical Laboratories Mayo Test ID: FIL5P SPH Sendout: must generate Mayo Clinic Order in MayoLink, 3050 Superior Drive NW see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG MML forwards to Viracor-IBT Lab QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Interleukin-6 (IL-6, IL6, NCODE 1 RED top 1 mL serum frozen in plastic vial. Ensure Bill 73 is completed Y Specimen Process Center interleukin 6, Cytokines) and copy of requisition for Sendout bench. Mayo Medical Laboratories *1 SST Gold top 3050 Superior Drive NW Mayo Test ID: FIL6S acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG MML forwards to Viracor-IBT Lab QTgyREYy%0A Send frozen on dry ice. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Interleukin-6, Highly Sensitive NCODE 1 LAV top 1 mL plasma frozen in plastic vial. Gel tubes not acceptable. Y Specimen Process Center Stability 210 days frozen, send on dry ice. Ensure Bill 73 is Mayo Medical Laboratories Mayo Test ID: FIL6H *1 RED top completed and copy of requisition for Sendout bench. 3050 Superior Drive NW acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= MML forwards to Quest Diagnotic NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Interleukin-8 (IL-8, Cytokines, NCODE 1 RED top 1 mL serum frozen in plastic vial. Stability is 28 days frozen. Y Specimen Process Center IL-8, interleukin 8) Send on dry ice. Ensure Bill 73 is completed and copy of Mayo Medical Laboratories *1 SST Gold top requisition for Sendout bench. 3050 Superior Drive NW Mayo Test ID:FIL8S acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= MML forwards to Viracor-IBT Lab NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Division of Pre and Post Examination, Page 154 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Interleukin-10 (IL-10, IL10, NCODE 1 RED top 1 mL serum frozen in plastic vial. Send on dry ice. Ensure Bill Y Specimen Process Center Interleukin 10, Cytokines) 73 is completed and copy of requisition for Sendout bench. Mayo Medical Laboratories *1 SST Gold top 3050 Superior Drive NW Mayo Test ID: FIL1S acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG MML forwards to Viracor-IBT Lab QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Joanne Luider Interleukin 12 (IL-12, CD212 NCODE 2 DRK GRN top Collect and ship by FedEx on Monday. Whole blood at room Y Calgary Laboratory Services, Flow protein expression, IL-12Rb1, Na hep temperature. Sample must arrive in Calgary on Tuesday. Cytometry pSTAT4 signalling) Complete Calgary Lab Services Out of Province requisition. McCaig Tower Rm 7509, Foothills Medical Centre Put Fax Result to SPH Lab request sticker on requisition or on th 1403-29 St. N.W. Sunset Printout. Calgary, Alberta, Canada T2N 2T9 Contact Performing Lab when test is picked up by FedEx. (403) 944-8995 Interleukin 28B (IL28B) NCODE TALL LAV 2 to 4 mL Whole Blood EDTA, minimum 2.0 mL. Send Specimen Specimen Process Center Polymorphism in original tube on Ambient temperature. Ensure Bill 73 is Mayo Medical Laboratories completed and copy of requisition for Sendout bench. 3050 Superior Drive NW Mayo Test ID:OIL28 SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Patient must complete Informed consent:

TB Interferon Gamma Release TBTS 6 mL DRK GRN Order only if special request (e.g. TBQS was already performed). Y – Medical BC Centre For Disease Control Assay (IGRA) (use only Lithium Heparin Specimen must arrive at BCCDC by noon on Monday, Tuesday Microbi. C/O PHSA Laboratories – Lane by special Or Wednesday before noon. Send whole blood. Package IGRA from Level Laboratory TB Interferon Gamma Serology request – separately and affix IGRA organ label to the package. Phone Hemodialysis (T Spot, TB Tspot, T-Spot) ZEP Lab: 604-707-2628 use TBQS BCCDC-ZEP to inform specimen is en route. or Renal for routine ***Write Collection Time on specimen label** patient is IGRA) preapproved.

Division of Pre and Post Examination, Page 155 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Iodine NCODE 3mL NAVY top Transfer into Falcon Polypropylene Tubes or contaminant free 10 days Y Inc. plasma tube. Approval required. Freeze and send frozen. Copy of 57 Gervais Dr. requisition for send out. Put Fax Result to SPH Lab request North York, ON M3C 1Z2 sticker on requisition or on Sunset Printout.

Additional information on HICL website: Phone 416-391-1499 Ext.248 http://www.HICL.on.ca/search_tcna.asp?Tcstring=IODINE%20P Fax Phone 416-385-1957

Iodine Urine (Free) NCODE 50 mL 24 HR Send frozen in plastic bottle on dry ice. 24 hr volume. Requested Y Specimen Process Center collection on requisition. If test is used in conjunction with 131 iodine Mayo Medical Laboratories Mayo Test ID: UIOD uptake. Approval required for all patients. 3050 Superior Drive NW Rochester, MN 55901 Additional information on Mayo website: 1-800-533-1710 http://www.mayomedicallaboratories.com/test-

catalog/specimen/9549 Ensure Bill 73 is completed and copy of requisition for Sendout bench. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Iron (Urine) – Test not available FEU Collected with 15 Collection bottle with plastic cap and funnel must be rinsed with Y VGH Lab as of Jan 2014. mL of 6mol/L HCL concentrated nitric acid and then deionized water. Urine must not in acid washed come in contact with metal. Send CRR result with specimen. container Hospitals In-Common Laboratory Islet Cell Antibodies, Pancreatic NCODE 1 RED top 2-4 mL RED top serum. Send frozen on dry ice. Copy of 7 days Y Inc. requisition for send out. Put Fax Result to SPH Lab request 57 Gervais Dr. sticker on requisition or on Sunset Printout. North York, ON M3C 1Z2 Additional information on HICL website: http://www.HICL.on.ca/search_tcna.asp?tcstring=panab Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 Isoniazid INZB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 156 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Isopropanol-Denaturation Or 3 mL EDTA Not available. See Hematology prior to collecting. Copy of Y SPH – Immunology Unstable Hgb plasma requisition to Hem.

Isopropanol Precipitation 3 mL EDTA Not available. See Hematology prior to collecting. Copy of Y SPH – Immunology plasma requisition to Hem.

Itraconazole ITRAB 1 RED top or Centrifuge ASAP, 2-4 mL serum or EDTA plasma, Freeze. Send N BCCDC Prov Tox on ice pack. Ask for dose time. Approval from Microbiology is 6 mL LAV top NOT required. Pre and Post dose could be requested. Pre dose is before the dosage. Post dose is 2-hour Post oral or 30 minutes Post IV. Sunquest Order Entry instructions: free Text in the following fields ITRDLD = ;DD MMM YYYY e.g. ;15 APR 2013 ITRTLD = ;HH:MM e.g. ;07:00

Japanese Encephalitis Virus JEV 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control Serology Top tube

Jo – 1 (Anti Jo-1; Anti-Jo1) ANAEB 1 SST Gold top Aliquot and Freeze. Send on ice pack. Stability: 3 days @ 4°C. VGH Lab

K (Potassium) K 1 PST 0.5 mL Lithium Heparin plasma. 24 Hour SPH Chem

K (Potassium), fluid FK 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid K not available. preservative container Division of Pre and Post Examination, Page 157 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Keflex Unavailable

Ketamine KETAB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Ketoprofen KETB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Ketorolac SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; KETOROLAC SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Kleihauer Test KLB 3 mL EDTA tube Give to Hematology. Daily SPH Copy of requisition to Hem.

L/S Ratio (Lecithin/Sphingo Unavailable. Revised Aug/03. Myelin)

Labetolol LABB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Lactate, CSF CSLA CSF 0.25 mL CSF supernatant 24 Hour SPH Chem.

Lactate Dehydrogenase LD 1 PST 0.5 mL lithium heparin plasma. 24 Hour SPH Chem (LDH, LD) Lactate Dehydrogenase, Fluid FLD 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem (FLD) top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF FTYPE RED top or non Synovial Fluid LD not available. preservative container 1 RED top Lamotrigine LAMOB 2 -4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center (Lamictal) dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be

separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack. >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Lamitrigine

Division of Pre and Post Examination, Page 160 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Lamuvidine Resistance Testing LAMRB 6 mL EDTA tube Do not spin or aliquot. Send whole specimen on ice pack. BC Centre For Disease Control Test no longer available. C/O PHSA Laboratories – Lane 04/2014 Level Laboratory

Lap Score (Leukocyte Alkaline Unavailable. Revised Aug/03 Phosphatase Stain

LCMV Serology-Lymphocytic LCMV 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Choriomeningitis

LDH, CSF CSFLD 1.0 mL CSF Freeze. Send on ice pack. VGH Lab

LDH Isoenzyme LDHI 5 mL SST Gold top 2 mL serum, divided into 2 tubes each containing 1 mL. Keep at Y Specimen Process Center room temp, stability: 7 days at ambient, 48 hours refrigerated. Mayo Medical Laboratories Mayo test ID: LD_i 3050 Superior Drive NW Rochester, MN 55901 Ensure Bill 73 is completed and copy of requisition for Sendout 1-800-533-1710 bench. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Lead (Blood) PBWB 1 Li Heparin DRK Follow Trace Metal Collection Protocol. Minimum 1.0 mL whole Y Children’s Hospital GRN top tube blood, mix well. Do not centrifuge. Store and send tube upright o at 4 C within 1 week of collection. Send copy of requisition with transport batch. Children’s Hospital Lab can use both Na and Li Hep non gel tubes: http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_20 140207_113746_Blood%20Collection%20for%20Trace%20Elem ents%20rev%20Dec%202013.doc

Division of Pre and Post Examination, Page 161 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Lead (Urine) PBU Collected with 15 Prior consultation and approval by C&W Lab Physician Y Children’s Hospital mL of 6mol/L HCL required. Refrigerate and send CRR result. See CH collection Add CRU, in acid washed protocol: (copy and paste path to Intranet browser) UTIM container \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Miscellaneous Collection pH 1.5 – 4.0 Procedures\Childrens_Hospital_24 HR URINE Collection Protocol March 9_09.pdf. Verify pH must be <4. Indicate on requisition: total urine volume, date and times of collection, and if collected on Acid washed container. Transport on ice by overnight courier to have sample arrive at C&W lab by 12:00 hrs (noon) the day after collection. Lebers Hereditary Optic 6 mL EDTA Prior consultation required. Keep and send at room temperature. Y Children’s Hospital – Biochemical Neuropathy NO LONGER TAT=12weeks. Copy of requisition for send out. Genetics Lab Room 2F22 AVAILABLE 11/2014 604-875-2307

Leflunomide, Serum NCODE 1 RED top Centrifuge Red top within 2 hours of collection. Freeze serum and Y Calgary Laboratory Services Teriflunomide send on dry ice. MSP Pre- Diagnostic & Scientific Centre Leflunomide Metabolite approval Assessment of elimination in patients requiring enhanced #9 3535 Research Way NW needed elimination of the drug in patients who wish to become pregnant. Calgary, AB T2L 2K8 Renal Outpatients with MSP Preapproval can have 2 specimens 403 770-3600 collected 2 weeks apart for monitoring therapeutic level. Additional information on CLS website: http://www.calgarylabservices.com/lab-services-guide/lab- tests/AlphabeticalListing/L/Leflunomide-Metabolite.htm

Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Legionella Antigen Test LEGAGB Random urine Refrigerate. Send on ice pack. BC Centre For Disease Control Refer out

Legionella Serology LEGIOB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Refer out

Leishmaniasis LEISHB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Leishmania Serology

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Leptospira PCR NCODE 5 mL SST Gold top Refrigerate. Send on ice pack. Ask BCCDC ZEP if other Y – TRAP BC Centre For Disease Control ZEP specimen types. Modify REFTO1 with Med Micro initials if Approved: E.g. REFTO1=;Sent to BCCDC-AMMMR

Leptospira-CFT (Blood Culture) LEPTCB 6 mL DRK GRN See microbiology for more info. BC Centre For Disease Control Heparin

Leptospirosis – Serology LEPTO 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control (BCCDC Sendout) Leptospira Serology

Leucine Aminopeptidase Unavailable, suggest gamma GT.

Leukocyte Antibody 1 RED top Not Available – July 24, 2013. SPH Transfusion Medicine (Blood Bank)

Levamisole DRSCB 10 mL urine Freeze. Sample should be stored at –20°C. Copy of requisition Provincial Toxicology Center (Levamisole is an impurity see for send out. in cocaine and would be instruction Sunquest LIS Order Entry instructions: identified when cocaine is *If collection time is now (N), enter time as one minute before in ordered) order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Levamisole

Levetiracetam (Keppra®) LEVETB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

LGV PCR non blood NCODE Rectal swab Y BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory Forward to NML Winnipeg

LGV Serology LGVR 5 mL SST Gold top Refrigerate. Send on ice pack. Copy of requisition for send out. Y BC Centre For Disease Control - Lymphogranuloma Venereum C/O PHSA Laboratories – Lane Requires (LGV NAT or LGV PCR) Level Laboratory approval before Forward to NML Winnipeg ordering

Lhon Dystonia Mitochondrial MDT 6 mL EDTA Prior consultation required. Copy of requisition for send out. Y Children’s Hospital – Biochemical Mutation Keep and send at room temperature. TAT=12weeks Genetics Lab Room 2F22 (AKA Lhond Mutation) 604-875-2307

Lidocaine LDCNB 1 RED top 2-4 mL serum or urine. Test is assayed Monday to Friday usually Y Provincial Toxicology Center (Zylocaine) before 1200 hours, available on a stat basis if absolutely necessary. Ward should record information on dosage & time of last dose. Specimen should be collected prior to next dose. Refrigerate. Send on ice pack.

Liley Curve For Bilirubin LCC 3 mL Amniotic Protected from light. Put our fax # 604 806 8815 on requisition. Children’s Hosp. Lab Pigments Fluid Copy of requisition for send out. Rm 2F40, 4480 Oak Street Amniotic Fluid Bilirubin, Amniotic Fluid Analysis for Hemolytic Disease of the Newborn

Division of Pre and Post Examination, Page 164 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Lipase LIPA 1 PST 0.5 mL lithium heparin plasma (serum acceptable), refrigerate. If 24 Hour SPH Lab frozen must send frozen.

Lipase, Fluid FLIPA 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid lipase not available. preservative container

Lipid Profile LIPID 1 SST Gold top 0.5 mL serum. 24 Hour SPH Chem (CHOL, HDLC, TRIG, LDLC) For fasting status enter PF if patient has been fasting 10-14 hours. Lipid Profile, Self Pay LIPSP 1SST Gold top If the patient has been fasting for less than 10 hours (or not at all) 24 Hour SPH Chem enter ;Fasting for X hours. For example, if the patient came in for bloodwork at 1000 and they had eaten at 0800 you would enter ;Fasting for 2 hours”. If the fasting status is unknown because the sample has been dropped off or sent in and the patient is unavailable to ask when they last ate enter UNAVOE.

Lipoprotein a LPA 1 SST Gold top or 1.0 mL serum, send frozen (dry ice preferred). Weekly SPH Chemistry 1RED top

Lipoprotein Electrophoresis PLEP 1 SST Gold top Collect after a 12 to 14 hour fast. Avoid alcohol for 24 hours before Mon – Y SPH (plasma lipoprotein sample collection. Refrigerate. Do not freeze. Fri Electrophoresis)

Lipoprotein 4 mL EDTA Do not freeze. SPH Ultra Centrifugation plasma NO LONGER AVAILABLE.

Listeria Serology LISTER 5 mL SST Gold top Refrigerate. Send on ice pack. Test not available 12/2017 BC Centre For Disease Control

0.5 mL serum. Collect prior to next dose – last dose time Lithium Level LITHL 1 SST Gold top 24 Hour SPH Chem required. Sunquest Last Dose format: LITDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE LITTLD: ;HH:MM e.g. ;13:50 or UNAVOE Lithium Erythrocyte 1 RED top and 1 mL serum and 2 mL whole blood. Through level should be Lions Gate Hospital drawn at least 12 hrs after dose. Indicate time last dose of lithium 1 LAV top given.

Division of Pre and Post Examination, Page 165 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Liver Function Test 1 PST Emergency department request LFT, tests include: ALKP, ALT, 24 hour SPH Chem LFT AST, GGT, LD and TBIL. Wards must specify which Liver Function tests to order.

Liver Kidney Microsomol (Type LKM1 1 SST Gold top 0.5 mL serum. Freeze. Send frozen on ice pack VGH Lab 1)

Long Acting Thyroid Stimulants (see Thyroid Receptor Antibody) Hospitals In-Common Laboratory Long Chain Fatty Acids NCODE 1 Tall Lav on ice For Adults: fasting overnight (12 hours), For children 1 – 12 yrs, Y Inc. Very Long Chain Fatty Acids; overnight fast preferred but minimum of 4 hrs is acceptable. For 57 Gervais Dr. C26, C26:C22 ratio,C24, infants < 1 yr, collect before next feed (2 – 4 hrs). North York, ON M3C 1Z2 C24:C22 ratio, phytanic acid, Centrifuge ASAP and separate 2 mL EDTA plasma and freeze. pristanic acid) Copy of requisition for send out. Ship frozen on dry ice. Put Fax Phone 416-391-1499 Ext.248 Result to SPH Lab request sticker on requisition or on Sunset Fax Phone 416-385-1957 Printout.

Lorazepam (Ativan) LRZPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 166 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

LSD Screen LCLSD 1 RED top 2-4 mL RED top serum. Test only upon prior consultation with Y Provincial Toxicology Center chief analyst. Refrigerate Serum. Send on ice pack.

Luteinizing Hormone LH 1 SST Gold top 0.5 mL serum in False Bottom or 13 x 75 Polypropylene tube. Mon & SPH Special Chemistry (Leuteotropic Hormone, Send with ice pack within 24 hrs of collection or send frozen. Thurs Pituitary gonadotropins +FSH) Lyme C6 Peptide Serology CPEPB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

BC Centre For Disease Control Lyme Disease Serology BBGMS 5 mL SST Gold top Refrigerate. Send on ice pack.

Lyme Disease Antibodies BBGMS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Borrelia) C/O PHSA Laboratories – Lane Level Laboratory

Lyme (European) Western LEWB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Blots C/O PHSA Laboratories – Lane Level Laboratory

Lymphocyte Crossmatch LYMXMB 1 RED top Keep specimen at room temperature. VGH Lab Date & time of collection must be on the specimens & on the Tissue Typing 2 YELLOW th requisition. 910 W. 10 A clinical diagnosis must accompany req.

Macro AST Not Available Not Available At Mayo Medical Laboratories – updated Apr/11

Macroprolactin MPROLB 3 mL LT GRN Refrigerate send on ice pack Y VGH Lab (Do NOT order PROL with this (LITH Heparin) test, VH will reflex PROL)

Magnesium MG 1 PST 0.5 mL lithium heparin plasma, refrigerate. 24 Hour SPH Chem

Division of Pre and Post Examination, Page 167 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Magnesium, Fluid FMG 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top , FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid magnesium not available. preservative container Refrigerate during collection. Measure 24 hour volume. Aliquot 3 Magnesium, 24 hour urine MGU 24 hour no Mon – SPH Chem mL for urine creatinine, centrifuge and aliquot supernatant to 12 preservative (Acid Fri Add CRU, x75 plastic tube. Aliquot approximately 100 mL from well mixed container UTIM 24 hour collection and adjust the pH of aliquot to pH 2-3 with 6 M acceptable) HCl (do not send whole collection) for heating at SPH. Referring sites need to include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack. Magnesium, random urine MGRU Random urine Order code includes urine creatinine and ratio. 3 mL aliquot, 24 Hour SPH Chem centrifuge and aliquot supernatant to 12 x75 plastic tube.

Magnesium, RBC NCODE 1 K2EDTA dark Minimum 2.0 mL whole blood. Spin down sample and discard Y LifeLabs blue top plasma, buffy coat and first layer of red cells. Replace primary (erythrocyte magnesium, RBC tube cap tightly and freeze red cells in primary tube. Send frozen Mg) on dry ice through Life Labs courier. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Not covered by MSP; cost is is $33.00. Give copy of receipt to billing.

Magnesium, Stool STMG Stool in non Liquid specimen required. Test within 4 hours if stored at room 24 Hour SPH Chem preservative temp., and within 24 hours if refrigerated. 0.5mL supernatant container required, more if Millipore filtration is required.

Malathion NCODE 2-4 mL serum – Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center RED top tube.

Malaria Stain MALS 1 EDTA 1.0 mL minimum fill for 3 mL EDTA Vacutainer. Only 1 order per 24 Hour SPH Hem (Malaria screen, Malaria thin day. Add and thick) CBCD

Malignant-Hyperthermia 1 RED top Order CPK cholinesterase and dibucaine

Division of Pre and Post Examination, Page 168 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Manganese, Serum (CW 1 DRK BLUE top Copy of requisition for Sendout. Follow Children’s Hospital Trace Y Children’s Hospital code no additive metal collection protocol, (search Zinc in eLab Handbook)

MNGS – Keep “no additive” sample at room temperature in an upright use only if position to clot. Allow at least 30 minutes. Do not expose approved sample to stopper any longer than necessary for collection. by Do not ring clot with applicator stick. Children’s Centrifuge clotted sample. Lab) Remove stopper. Transfer serum or plasma into a polypropylene tube and cap immediately. Hemolyzed specimens are not suitable for serum Mn. Store at 4ºC for periods of up to one week. Freeze at -20ºC or lower for longer periods of storage to slow down possible leaching and/or adsorption. The minimum volume required of either whole blood or serum/plasma sample is 1 mL. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Hospitals In-Common Laboratory Manganese, Whole Blood MNG 1K2EDTA Dark Obtain Dark Blue K2EDTA tube from Supervisor’s Office. 10 days Y Inc. blue top Follow Children’s Hospital Trace metal collection protocol (search 57 Gervais Dr. Zinc in eLab Handbook). *Special Collection North York, ON M3C 1Z2 Use another dark blue K2EDTA tube as a primer if possible. tube from Supervisor* Mix well, store tube upright at 4°C. Do not centrifuge whole blood. Phone 416-391-1499 Ext.248 Transport upright on ice to C&W lab within one week of collection. Whole blood may be frozen. Fax Phone 416-385-1957 Send to HICL if not approved by CW: (Only send to Children’s Hospital http://www.hicl.on.ca/search_tcna.asp?TCString=MANGA%20WB under Special Approval from CW) Copy of requisition for Sendout. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Maprotyline (Ludiomil) MPRTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center (tetracyclic Antidepressant) requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Maternal-Antibodies 6 mL EDTA + If patient injected with RH gamma globulin note on form. CBS via SPH TM 10 mL blood

Maternal Investigation MI 1 Tall LAV Same as FBSPHC. Copy of requisition to Blood Bank 24 SPH Transfusion Med. hours. Routine: 1-3 hrs STAT: 1hr

Measles – IgG MIGB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - Rubeola IgG Serology C/O PHSA Laboratories – Lane Level Laboratory

Measles – IgM and IgG MEASP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - Rubeola IgM and IgG C/O PHSA Laboratories – Lane Serology Level Laboratory

Measles PCR non blood MISPCR Swab, urine Y BCCDC (measles culture) Meat Fibers in Feces MFIB Fresh random stool Test no longer available 12/2017 Daily SPH Chem

Meconium DRSCB Baby sample in Refrigerate. Send on ice pack. Y Provincial Toxicology Center sterile container

Meclozine NCODE 2-4 mL serum – Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center RED top tube.

Melas Mitochondrial Mutations 6 mL EDTA Prior consultation required. Copy of requisition for send out. Y Children’s Hospital – Biochemical Keep and send at room temperature. TAT=12weeks Genetics Lab (AKA Mitochondrial Room 2F22 Encephalomyopathy, Lactic Acidosis, Stroke-Like TEST NO LONGER AVAILABLE. 11/2014 604-875-2307 Episodes, Mitochondrial Melasmutation)

Meningococcal Serology MENS Indicate specimen BC Centre For Disease Control type at RESOE (SD0151)

Meperidine (Demerol) MEPEB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 170 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Division of Pre and Post Examination, Page 171 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Mercury (Blood) HGWB 1 DRK GRN top Li Follow Trace Metal Collection Protocol. Minimum 1.0 mL whole Y Children’s Hospital Heparin tube. blood, mix well. Do not centrifuge. Store and send at 4oC within

1 week of collection. Send copy of requisition with transport

batch.

http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_20 140207_113746_Blood%20Collection%20for%20Trace%20Elem ents%20rev%20Dec%202013.doc

Mercury (24 Hour Urine) HGU 24 HR. Urine in 15 Minimum 10 mL aliquot. All equipment used must be acid Y Children’s Hospital mL. Of 6mol/L cleaned e.g. Glassware, aliquot bottles. Add CRU, HCL acid cleaned See CH collection instructions: (copy and paste path to Intranet UTIM bottle. browser) \\vch\departments\Chemistry (Dept MIN. 50 mL. PHCLAB)\Accessioning\Miscellaneous Collection Ph 1.5 – 4.0 Procedures\Childrens_Hospital_24 HR URINE Collection Protocol March 9_09.pdf Mercury (Random Urine) HGRU 50 mL urine Order code includes urine creatinine and ratio. Y Children’s’ Hospital Add CRR

Merkel Cell Panel, Anti Merkel NCODE 1 RED top 2 mL of serum (minimum 0.5 mL), freeze. Before you collect: University of Washington Medical Cell panel Center • Charge $150.00 for shipping. Patient must arrange for 1959 NE Pacific St, payment with University of Washington prior to blood Seattle, WA 98195, USA being drawn (206)685-6066 OR • charge $365.00 for cost of test (price includes shipping). Patient must sign Bill 73 form. Copy of AMERK requisition for sendout. Add Phlebotomy fee if no other tests ordered to be done at SPH. Sendout person: Send specimen on dry ice with AMERK Requisition Merrf Mitochondrial Mutations 6 mL EDTA Prior consultation required. Copy of requisition for send out. Y Children’s Hospital – Biochemical Keep and send at room temperature. TAT=12weeks Genetics Lab (AKA Myclonic Epilepsy) Room 2F22 NO LONGER AVAILABLE. 11/2014 604-875-2307

Division of Pre and Post Examination, Page 172 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Mesantoin (Mephenytoin) MPHNB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Mesoridazine (Serentil) MESORB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Metanephrines, plasma NCODE 6 mL EDTA Pre-approval is required. Y Calgary Laboratory Services (Fractionated plasma Patient must fast and abstain from smoking for at least 4 h prior Diagnostic & Scientific Centre Metanephrines) or (Free to collection. Separate into 2 aliquots and freeze. Store and #9 3535 Research Way NW Metanephrines) send frozen on dry ice. Calgary, AB T2L 2K8 403 770-3600 Additional information on CLS website: http://www.calgarylabservices.com/lab-services-guide/lab- tests/AlphabeticalListing/M/Metanephrines-Plasma.htm Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Metformin METFB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 173 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

hours Post)

Methadone In Urine MTD Random Urine >1.0 mL urine. Refrigerate if not done immediately. Order UDS 24 Hour Y – if DRSCB SPH (Outpatients) if screen ordered. Add DRSCB if Dr specifies ordered. Dolophine part of Methadone confirmation (or aka names) and give copy of EDDP UDS and requisition for Medical Biochemist approval. 2-Ethylidene-1,5-Dimethyl- UDP 3,3-Diphenylpyrrolidine panel

Methadone In Urine, DRSCB Random urine Refrigerate. Send 50 mL Urine on ice pack. Y Provincial Toxicology Center Confirmation Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Methadone confirmation

Methamphetamine (Ice) MEPHB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 174 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

(Serum is Medical Biochemist for approval. See instructions in Stat Test – Process 875 4111 Local 68203 acceptable) methanol/ethylene glycol section Immediately Tightly Stoppered.

Methapyrilene SPQ 1 RED top 2-4 mL serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Methapyrilene SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Methaqualone (Quaalude) MTQB 1 RED top 2-4 mL serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Methaqualone (Urine) UDRG urine 50 mL Refrigerate urine. Send on ice pack. Copy of requisition for send Y VGH Lab Qualitative. out.

Methemoglobin FMHB 1 LAV/Syringe Sample must be fresh. Store at room temp. 24 Hour SPH Chem

MSJ: 1 MSJ Lab PST/Syringe

Division of Pre and Post Examination, Page 175 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Methocarbamol (Robaxin) MCBB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Methocarbamol Methotrexate MTXB 1 RED top 1 mL serum (non-gel heparin plasma acceptable). CSF and Children’s Hospital EDTA plasma also acceptable. Remove Serum or plasma from cell within 1 hour of collection (protection from light is not DO NOT USE GEL required). SEPARATORS. Obtain last dose information. Test code includes dosage, time and date of last dose. Specimens must be sent same day as collection. If the afternoon BCCH courier is missed, batch samples and send by cab prior to the end of shift (1500 or 2300), 7 days a week.Send immediately by cab If ordered STAT, affix pink stat label. Refrigerate (up to 7 days), send on ice pack. Frozen suitable for up to 1 month. 1 RED top Methotrimeprazine (Nozinan) MTTPB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Methotrimeprazine Methsuximide (Celontin) MTHSB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 176 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Methyl Salicylate SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Methyl Salicylate SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Methylphenidate (Ritalin) MTPDB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 177 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Metoprolol (Lopressor) METOB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 178 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Mexitil (Mexiletine Hcl) MXLT 1 RED top 2 mL serum. Refrigerate Serum. Send on ice pack. Copy of Y Victoria General Hosp requisition for send out. Put Fax Result to SPH Lab sticker. Tel: 727-4167

Microalbumin, Random Urine MALBR Random urine Refrigerate during collection. 50 mL aliquot from 24 hour Mon – SPH Special Chem collection (do not send whole collection). Referring sites need to Fri (Albumin-Urinary-Microalbumin) include 24 hour volume, patient’s height and weight on requisition. (Microalbumin 24 hr urine no Refrigerate and send on ice pack. Do not freeze! Albumin/Cre Ratio) preservative. Must MALBU not be bloody or in Microalbumin, 24 Hour Urine add UTIM Acid

Micro Polispora faeni No longer available. Please order FTVUL Thermophilic Vulgaris 1 RED top Midazolam MIDZB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 179 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Midazolam 1 RED top Mirtazapine MIRB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Mirtazapine Miscellaneous CGL Test MCGL Na Heparin Orderable by CW CGL only. Children’s Hospital Miscellaneous Cytogenetics Test, FISH

Mitochondrial DNA Deletions NCODE 1X 6 mL EDTA Prior consultation required. Copy of requisition for send out. Y Children’s Hospital – Biochemical Keep and send at room temperature. TAT=12weeks Genetics Lab (AKA Mitochondrial Room 2F22 Deletions, MTDNA Deletion, Long Range PCR 604-875-2307 Mutations)

Mitogen-Virol-Stimulation 30 mL Heparin Prior consultation required. Must arrive at Children’s Hospital Y Children’s Hospital – Biochemical blood before noon. Copy of requisition for send out. Genetics Lab TEST NO LONGER Room 2F22 AVAILABLE. 11/2014 604-875-2307

Mixing Studies, PT PTS 3 CIT Full citrate draw. Determine if patient is on oral anticoagulant. 24 Hour SPH Coag Specimen must be centrifuged within 2 hours of collection. Minimum 3.0 mL plasma double spun, aliquot in polypropylene tube. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy requisition for Special Coag.

Division of Pre and Post Examination, Page 180 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

MMR Serology MUIGB, 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Includes MIGB, MUIGB And MIGB and C/O PHSA Laboratories – Lane RUBEB) RUBEB Level Laboratory - Mumps, Measles And Rubella

Moclobemide (Mannerix) MOCBB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Moclobemide Miscellaneous MGL Test MMGL Orderable by CW MGL only Children’s Hospital Molecular (Molecular Genetics Lab) Genetics Lab (MGL)

Molecular Genetics for Children’s Hospital 1 x 6 mL EDTA Minimum 1.0 mL Whole Blood. Copy of requisition for Sendout. Y Children’s Hospital Molecular Genetics Lab (MGL) Achondroplasia Lavender top Approval unless specified Ordering Dr must complete Molecular Genetics Laboratory C&W Alloimmune requisition: required for 4500 Oak Street thrombocytopenia Inpatients Vancouver, BC MDT Alpha Thalassemia http://www.elabhandbook.info/phsa/Files/RequisitionForms%2f1 only. Specimen Receiving Room 2J20 _20140722_042203_CWMG_REQ_0000_v4.2_General_Requis Androgen Insensitivity Syndrome ition%20edit.pdf Angelman Syndrome Alpha and Beta Molecular Genetics Send at Room Temperature unless specified. Ashkenazi Carrier Thalassemia: Screening No approval if Beta Thalassemia ordered by Brugada Syndrome Drs.: CADASIL Ezzat, Charcot-Marie-Tooth Type Foltz 1A S. Jackson, Chimerism C.Leger, Cystic Fibrosis H.Leitch, Dystonia early onset Ross(Boldt), primary Hoeshi, P. Yensen, H. Dystrophinopathies Division of Pre and Post Examination, Page 181 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Facioscapulohumeral Merkley & Dystrophy Ramadan Familial Mediterranean Fever FMR1 Related Disorders Friedreich Ataxia GLUT1 Deficiency Syndrome Hemoglobin S,E,C Hemophilia A Hemophilia B Hereditary Neuropathy HFE Related Hemochromatosis Huntington Disease Hyper IgD Syndrome Hyperkalemic Periodic Paralysis Hypochondroplasia Hypokalemic Periodic Paralysis Kell Hemolytic Disease of Newborn Loeys-Dietz Syndrome Long QT Syndrome Marfan Syndrome Miscellaneous MGL Test Muenke Syndrome Myotonic Dystrophy Type 1 Oculopharyngeal Muscular Dystrophy Prader-Willi Syndrome Molecular Genetics RhC Hemolytic Disease of Newborn RhD Hemolytic Disease of Newborn RhE Hemolytic Disease of Newborn Sensorineural Hearing Loss Division of Pre and Post Examination, Page 182 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Spinal Muscular Atrophy Spinobulbar Muscular Atrophy Spinocerebellar Ataxia Panel Steroid 5-alpha-reductase deficiency Thanatophoric Dysplasia Transthyretin Amyloidosis TRAPS Uniparental Disomy UPD Weaver Syndrome 2 X-linked hyper IgM Syndrome X-linked Ichthyosis Zygosity Molybdenum, Urine MBR Random urine Collect and transfer in metal free container. Refrigerate and send Y Children’s Hospital on ice pack. Add CRR

Molybdenum, Whole Blood MBWB 6 mL DRK GRN Li Call Children’s before collecting test if test is performed. Follow Y Children’s Hospital Heparin Trace Metal collection protocol. Do not spin. Store upright in fridge and send upright with ice pack. Send copy of requisition with transport batch.

Mono Test MT 1 SST Gold top 0.5 mL serum. Refrigerate. 24 Hour SPH Hem

Monoacetylmorphine SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; MONOACETYLMORPHINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 183 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Morphine MRPNB 1 RED top 2-4 mL serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Morphine In Urine OPSC Random urine >1.0 mL urine. Refrigerate if not done immediately 24 Hour SPH Part of UDS and UDP panel

Morphine In Urine, Confirmation DRSCB Random urine Refrigerate. Send 50 mL Urine on ice pack. Y Provincial Toxicology Center Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED I default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Morphine confirmation

Morphology, RBC AMOR, 1 LAV 1.0 mL minimum draw for 3 mL EDTA tubes. Slide must be 24 Hour SPH Hem (slide review, blood smear) add CBC prepared within 6 hours of collection.

Morquio A,WBC WMORA 6 mL Green Prior consultation and approval by Lab Biochemical Geneticist is Y Children’s Hospital Lithium Heparin required. N-acetyl galactosamine 6- Whole blood must arrive at C&W within 3 hrs of collection and prior sulphatase, Galactose 6- to 1200 hrs. If this is not possible, refer patient to C & W hospital sulphatase, Outpatient Laboratory for collection. Mucopolysaccharidosis type IVA, MPS type IVA Keep and send at room temperature.

Division of Pre and Post Examination, Page 184 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Mucopolysaccharide, Urine MPSU 24 HR. Urine, no After removing aliquot for CRR, transfer min 2 ml to a 5 ml tube Children’s Hospital – Biochemical Add CRU, preservative and ship frozen. See Children’s Handbook for detail. Genetics Lab UTIM Room 2F22 604-875-2307 MPSU 50 mL random Add CRR urine

Mumps – IgG MUIGB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - Mumps Serology C/O PHSA Laboratories – Lane Level Laboratory

Mumps –Anti Mumps IgM and MUMPS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control IgG C/O PHSA Laboratories – Lane Level Laboratory

Mumps PCR (non blood) MISPCR Swab or 50 mL of random urine in sterile container. Refrigerate urine. Y – Med BC Centre for Disease Control Must have Med Micro Approval. Micro 50 mL urine

Murray Valley Encephalitis NCODE 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control Virus (BCCDC Sendout) (BCCDC code ZMBAT)

Mycophenolic Acid (MPA) or MPA 1 EDTA 1.0 mL EDTA plasma. Send frozen on dry ice. Ask for dose time. VGH Lab Mycophenolate (Cellcept) No approval required

Mycoplasma Antibody MYCOB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Mycoplasma Pneumoniae IgM) Level Laboratory

Mycoplasma pneumoniae NAT CMPN Genital Swab or Forward specimen to microbiology to refer out. Children’s Hospital Microbiology MSU Mycoplasma pneumoniae PCR Must send copy of requisition with transport batch. 1 RED top Hominis Ureaplasma Urealyticum, CSF

Mycoplasma Pneumoniae IgM MYCOB 5 mL SST Gold top Do not freeze. Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Division of Pre and Post Examination, Page 185 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Myelin Associated Glycoprotein AM1 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler (anti-MAG, MAG antibody) send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

Myoglobin, Urine (urine MYOG 10 mL Fresh urine No longer available as of 12/2017 24 Hours SPH Chem myoglobin) RMU CK

N-Acetyl Procainamide PROCB 1 RED top 2-4 mL serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

N-Methylhistamine, Urine NCODE 24-hour urine Must have MSP Approval letter prior to collection. Y Specimen Process Center collection Mayo Test ID:NMHIN CRU 1. Ask patient to sign Bill73 consent form. Mayo Medical Laboratories No preservative 3050 Superior Drive NW UTIM 2. Instruct patient to refrigerate urine during 24 hour collection. preferred. (see Rochester, MN 55901 Mayo for other Accessioning: processing 24 hour urine: 1-800-533-1710 preservatives). 1. 1 aliquot for CRR 2. 1 aliquot for NMHIN, spun and aliquot 5 mL (minimum 3mL), freeze immediately. Stability: Refrigerated 8 days, Frozen 14 days, Ambient 24 hours SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor, provide total 24 hour volume in Comments section of MayoLink Test orders.: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Send frozen on dry ice.

Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.).

Division of Pre and Post Examination, Page 186 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 1 SST Gold top C-Telopeptide NCODE Ask patient to pay at Cashier and make 2 copies of payment LifeLabs. (CTx) – not covered by MSP Patient must be receipt, one stapled with copy of requisition for Send Out Patient must pay fasting Tech, the second stapled to original requisition. $65 for Random CTx or Refrigerate serum and send on ice pack. Additional information: $130 for Baseline CTx. http://tests.lifelabs.com/BC/Chemistry/C_TELOPEPTIDE_RAND OM/C_TELOPEPTIDE_RANDOM__Lower_Mainland.aspx?s=1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

N-Telopeptide NCODE Serum No longer available. Revised Sept. 2011

N-Telopeptide (Urine) NCODE 2x 5 mL aliquot 24 Hour: Submit two 5 mL aliquots from 24 hour collection. Y Calgary Laboratory Services from no Provide collection date and 24 hour urine volume on req. (CW code Diagnostic & Scientific Centre preservative 24h NTEL Random sample: second morning urine collected 7:30 – 10:30 #9 3535 Research Way NW collection preferred blocked a.m. Provide collection date. Indicate Random. Calgary, AB T2L 2K8 from SPH) Or 2 x 5 mL from 403 770-3600 Store and send frozen. If the specimen thaws, it is unsuitable for second void in the analysis. Send frozen on dry ice. Approval required for all morning patients.

Additional information on CLS website: http://www.calgarylabservices.com/lab-services-guide/lab- tests/AlphabeticalListing/N/N-Telopeptide.htm Copy of requisition for send out. Must complete CLS requisition: http://www.calgarylabservices.com/files/HealthcareProfessionals/ Requisitions/REQ9012PSC.pdf Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Nabilone SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; NABILONE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

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Nadolol NADB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Naproxen NAPB 1 RED top 2-4 mL serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

NARP Mitochondrial Mutations NCODE 6 mL EDTA Prior consultation required. Copy of requisition for send out. Y Children’s Hospital – Biochemical (AKA Neurogenic Muscle Keep and send at room temperature. TAT=12weeks Genetics Lab Room 2F22 Weakness, Neuropathy, Ataxia, NO LONGER AVAILABLE. 11/2014 604-875-2307 Retinitis Pigmentosa)

Natural Killer cytotoxicity IDEF 1 GRN, 1 LAV Copy of requisition for Flow Cytometry Mon – SPH Hem (NK cell,NK assay, NK function) add Fri *ACD(A) also CBCD acceptable* Nefazadone NEFAZB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Netilmicin Not available

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Neuron Specific Enolase (NSE) NCODE 1 RED top 2 mL serum. Send frozen on dry ice. Ensure Bill 73 is Y Specimen Process Center completed and copy of requisition for Sendout bench. Mayo Medical Laboratories Mayo Test ID: NSE *1 SST Gold top 3050 Superior Drive NW acceptable* SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Additional information on Mayo website: http://www.mayomedicallaboratories.com/test- catalog/specimen/80913 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Newborn Screening NBCL NBS Card Optimal 4 dots, minimum 3 dots. Follow collection instructions on BC Children’s Hospital reverse of newborn screening card. Apply patient barcode to Newborn Screening Laboratory aka PKU screen, Neonatal Whatman 903 NBS back of NBS card. 4480 Oak Street, Room 2F27 Screen, Phenylalanine card (Newborn Do NOT use the pneumatic tube system to transport wet Vancouver, BC V6H 3V4 Ketone Urea Screen, Blood Screen cark, Blood bloodspot cards. Wet bloodspot cards must NOT be packaged Spot Screen, Blood Dot Dot Card) in biohazard bags. Screen, NBS, Newborn

Metabolic Screen Capillary collections preferred. Arterial and venous samples acceptable. Send on Transport Batch at room temperature.

NMDA Receptor Antibodies NMDAB 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, 7 – 10 Y Dr. Fritzler (Autoimmune Encephalitis) send frozen on dry ice. days Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582 Neuromyelitis Optica NMOB 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Autoantibody (NMO) send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top (Aquaporin, Devic’s Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the Antibody) HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

NCL,Infantile INCL NBS Card Optimal: 4 spots on Blood Dot Card, minimum 2 spots. Allow card Children’s Hospital to dry flat on level surface for at least 3 hours prior to packaging Neuronal Ceroid Lipofuscinocis, for shipment. PPT and TPP, Palmitoyl Do NOT use the pneumatic tube system to transport wet protein thioesterase and bloodspot cards. Wet bloodspot cards must NOT be packaged tripeptidyl peptidase, INCL, in biohazard bags. LINCL, CLN1 gene, CLN2 Identify the test clearly on card. gene, Infantile NCL, Late Ship at room temperature. Infantile NCL, Ceroid lipofuscinosis type 1

Neurotransmitters, CSF NTRAN CSF Contact Children’s Hospital. Special handling instructions Children’s Hospital – Biochemical required. CSF collection tubes containing preservative are Genetics Lab Room 2F22 CSF Neurotransmitters, 5- obtained from Biochemical Genetics, Children’s Hospital. Methyl-Tetrahydrofolate 604-875-2307 Transport specimen to Children’s Hospital via Dynamex Courier to ensure door to door delivery. Send copy of requisition with transport batch.

Neutrophil Antibody Not available as of Oct 1/94

Niacin (Vitamin B3) Not available Hospitals In-Common Laboratory Nickel, whole blood NCKL 1K2EDTA Dark Not covered by MSP, self pay for Outpatients. Do NOT open 10D Y Inc. blue top tube. Store and send whole blood cold. Put Fax Result to SPH 57 Gervais Dr. Lab request sticker on requisition or on Sunset Printout. *Special Collection North York, ON M3C 1Z2 tube from Supervisor* Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957

Nicotine (Serum) NICB 1 RED top 2 mL serum from RED top. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Nicotine (Urine) DRSCB 10 mL urine Refrigerate urine, send on ice pack. Y Provincial Toxicology Center see Sunquest LIS Order Entry: instruction *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Nicotine

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Niemann Pick Type C NCODE 6 mL EDTA Minimum volume 1.0 mL. Do not collect unless patient has Y Specimen Process Center (NPC, Niemann-Pick C) MSP pre-Approved funding letter. Test performed on Thursday Mayo Medical Laboratories at Mayo, specimen must arrive within 96 hours of collection: Send 3050 Superior Drive NW Mayo Test ID: NIEM at room temperature to Mayo on Monday or Tuesday only. Rochester, MN 55901 (Diagnosis of Niemann-Pick 1-800-533-1710 disease type C) http://www.mayoreferenceservices.org/it- mmfiles/MolGenBiochemicalDisordersInfoSheet.pdf Patient must sign Mayo Consent form. http://www.mayomedicallaboratories.com/it- mmfiles/Informed_Consent_for_Genetic_Testing_mc1235-117.pdf Ensure Bill 73 is completed and copy of requisition for Sendout bench. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Nipride Unavailable

Nitrofurantoin SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Nitrofurantoin SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Nitrazepam (Mogadon) NTRZB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Nomifensine SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Nomifensine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Non-invasive Prenatal Testing NIPTB Special Kit in Please follow NIPT procedure in Outpatient Resource binder: Gamma Dyna Care 10451 Shellbridge Way Ste 140 (NIPT, Harmony™) Outpatient cabinet 1. Order using separate Accession above hand wash Richmond, BC V6X 2W8 sink 2. Collect both tubes from the Harmony™ kit 3. Label with NIPTB barcodes 4. Receive CID in Sunquest 5. Forward all contents in kit to Sendout person

Sendout Person: 1. Send within 72 hours of collection (Stability is 7 days). Form Sunquest Transport Batch with NIPTB CID 2. Label tubes again with Harmony™ kit barcodes, covering Sunquest barcode but leaving patient demographics visible. 3. Send with FedEx Waybill included in Harmony™Kit. 4. Result PGMDNT worksheet same day. Result PGMDNR worksheet with RGMDYN English text code.

Norovirus Inpatient PVNOR Stool Forward Inpatient samples to SPH Virology. Send all Outpatient SPH Virology PCR samples to BCCDC with copy of requisition. Outpatient BCCDC

Nortriptyline NORTIB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug (Aventyl) by HPLC monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

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Norvenlafaxine SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; NORVENLAFAXINE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

NTBC Level NTBCL 6 mL Lith Heparin 1 mL plasma freeze and send on dry ice. Children’s Hospital Orfadin, Nitisinone, Green top on ice Tyrosinemia type I monitoring

Occult Blood OB Feces on OB test is only for Inpatients. Do not give to Outpatients. Order SPH Chemistry Urinalysis Hemoccult slides FIT for outpatient occult blood tests.

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OK T3 Antibody OKT3 1 – 2 mL. Serum or Refrigerate specimen. Patient information sheet from the ward ?CW forward to OHSU? plasma must accompany specimen. OKT3 Antibody Testing Lab. Send out within 7 days after being drawn. Research B-333 Ortho Mandatory MSP pre-approval required for Outpatients Pharmaceutical Corp. (request to be done by patient Dr.). For Outpatients, do not 1000 Route 202 collect without MSP preapproval if test is ordered alone. Raritan, New Jersey 08869-0602 Tel(908) 704-4490 Oligoclonal Banding, CSF OLIA 2-4 mL CSF Ensure plasma (or Serum) is available within 2 weeks. If VGH Lab add preferred plasma/Serum is unavailable, then have it collected. (IgG Synthesis Index) CSFTP (absolute min. = Send frozen both plasma and CSF (or Serum & CSF) Oligoclonal Banding, 1.5 mL CSF) together. Plasma/Serum (Log In & OLIAS) OLIAS 1.0 mL Lithium IGGP and ALB to be done at SPH. Heparin Plasma

preferred, GOLD Also Serum acceptable Order (absolute min.= 0.5 serum mL for VH) ± 2 IGGP, weeks of csf ALB. collection is acceptable Plus 1 SST Gold top for SPH

Oligosaccharides OLG 10 mL random Freeze, send frozen. Children’s Hospital – Biochemical urine Genetics Lab Urine Oligosaccharides, Urine Room 2F22 Sialic Acid, Sialic Acid,Aspartyl Glucosamine 604-875-2307

Olanzapine OLANB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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ORAP Not available

Organic Acids ORGS 20 – 25 mL. Urine Concentrated morning preferred. Urine MMA (methylmalonic Y-Adults Children’s Hospital – Biochemical (Methyl Malonic Acid Orotic acid) requests are only done on children (<19 yrs) or KNOWN Genetics Lab Add CRR Minimum 5 mLs N- Newborns acid, Homogentisic acid, N- patients with methylmalonic aciduria. Test is not offered for Room 2F22 (send STAT) acetyl aspartic, adipic, diagnosis of B12 deficiency in adults. 604-875-2307 branched chain ketoacids, After removing aliquot for CRR, transfer min 5 ml to a C&S dicarboxylic acids, container and ship frozen. ethylmonic, glutaric, hexanoyl glycine,4- Specimen must be kept frozen at all times. Process STAT. hydroxybutyric Contact Medical Biochemist for approval. Send copy of pyroglutamine acids, β- requisition with transport batch. hydroxy β-methylglutaric, isovaleric, ketones, lactic acid, methylcitric acids, methylcrotonyl glycine, methylmalonic, propionic, sebacic, suberic, suberylglycine, succinyl acetoacetate, succinyl acetone, Succinylacetone, valproate metabolites and others.)

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Orphenadrine (Norflex) ORPHB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Osmolality, plasma OSMS 1 PST 0.5 mL plasma. Refrigerate. 24 Hour SPH Chem (OSMC, calculated OSM reflex ordered if ALC, GLUC, NA, and URE ordered )

Osmolality, Stool (stool SOSM Liquid Stool in non Liquid specimen required. Test within 4 hours if stored at room 24 Hour SPH Chem osmolality) preservative temp., and within 24 hours if refrigerated. 0.5mL supernatant add STMG, STNA, STK container required, more if Millipore filtration is required.

Osmolality, Urine OSMU Urine in sterile 2.0 mL minimum. Refrigerate 24 Hour SPH Chem container

Ova and Parasites, Feces POPI Stool in SAF Ordered and Processed by Microbilogy BCCDC (Stool O&P) Fixative

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Oxalate, Plasma POXB 1 tube Na Heparin Patient must fast for 12 hours, and should avoid taking Vitamin C Y Specimen Process Center (GREEN top) place for 24 hours prior to draw. Mayo Medical Laboratories Mayo Test ID: POXA on ice and deliver 3050 Superior Drive NW Collect on ice, centrifuge within 1hr of collection at 2000 RPM at immediately to lab ° Rochester, MN 55901 4 C. Adjust plasma to pH of 2.3-2.7 (with approx 10 µL of 12N 1-800-533-1710 HCl per 1 mL of Plasma) and send frozen to Mayo. Stability is

only 7 days frozen, send ASAP. Additional information at Mayo Website: http://www.mayomedicallaboratories.com/test- catalog/Specimen/81408 Ensure Bill 73 is completed and copy of requisition for Sendout bench. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Oxalates (Urine) OXU 24 HR. Collection Acidify with HCL to pH <2.0. Mix well, measure volume, aliquot VGH Lab in 15 mL. Of and freeze. Send on ice pack. 6mol/L HCL

Oxazepam (Serax) OXZPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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All requests on patients over 16 years, must be approved by a Hematopathologist before testing.

Oxprenolol OXPRB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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Oxycodone Confirmation, Urine DRSCB 10 mL urine Outpatients: order OXYS unless requested by Biochemist. Y Provincial Toxicology Center see Refrigerate urine. Send on ice pack. Copy of requisition for send instruction out. Sunquest LIS Order Entry:

*If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED Idefault tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Oxycodone Oxycodone, Urine screen OXYS Random urine >1.0 mL urine. Refrigerate urine. Send to SPH on ice pack. SPH Chemistry Part of UDS and UDP panel

Oxygen Dissociation P50 PICO Blood Gas Special collection instruction required. See collection of venous 24 Hour SPH Chem Blood Gas Syringe with whole blood p50 at Blood Gas bench. (Oxygen Affinity Or P50) electrolyte balanced heparin.

Oxyphenbutazone (Tandearil) OXYPB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Oxysterols OXSTR 1 EDTA Centrifuge and aliquot 1 mL EDTA plasma, freeze and send Y Children’s Hospital (Biomarkers for Niemann-Pick frozen. C&W will forward. C, NPC) Call Children’s Hospital – test available ~2015.

Paliperidone SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: (Hydroxyrisperidone) SD0177=SER or ;Urine SPQ1=;Paliperidone SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Pancreatic Islet Cell Antibodies NCODE (see Islet Cell Antibodies) Pancreatic Polypeptide NCODE Prechill Tall EDTA Patient must be fasting for 8 hours. 3.0 mL EDTA collected on Specimen Process Center LAV collected on ice. No gross hemolysis or lipemia. Minimum 0.35 mL frozen Mayo Medical Laboratories Mayo test ID: HPP ice plasma, stable for 90 days. 3050 Superior Drive NW Rochester, MN 55901 Mandatory MSP pre-approval required for Outpatients 1-800-533-1710 (request to be done by patient Dr.). For Outpatients, do not

collect without MSP preapproval if test is ordered alone. Ask patient to sign Bill73 form. Order Mayo Test: https://orders.mayomedicallaboratories.com/ Additional information on Mayo Clinic website: http://www.mayomedicallaboratories.com/test- catalog/Specimen/8014

Paracoccidioides Serology PACOC 5 mL SST Gold top Refrigerate. Send on ice pack Y –TRAP BC Centre For Disease Control (Paracoccidioides Antibody) Modify BCCDC’s Specimen Description with Med Micro initials if by Medical

TEST NOT AVAILABLE at Approved: E.g. SD0100=BLD-AMMMR Microbiologist referral Lab 5/2014, check with BCCDC ZEP before collecting

Paragonimus Serology PARAG Indicate specimen Refrigerate. Send on ice pack. BC Centre For Disease Control type at RESOE. C/O PHSA Laboratories – Lane Level Laboratory

Parainfluenza-Blood 5 mL SST Gold Test Not Available. SPH Virology Lab Top tube

Paraldehyde PARDB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Parasite, Macroscopic Exam PMACRO Results SPH Microbiology (Worm or insect ID) in 24 hours

Parathion Not available

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Parathyroid Hormone, Intact PTHI 5 mL SST Gold top Prefer 8 hours fasting. Spin and separate 1.0 serum ASAP in Serum: SPH Special Chemistry (PTH, Intact; parathyrin) False Bottom or 13 x 75 Polypropylene tube. Freeze and send 24 Hours on dry ice.. Fine For Fine Needle Aspirate: Needle *MSJ - 1 SST Aspirate: GOLD top. Can be Indicate collection site at Order Entry Modifier field. Mon - Fri stored and Freeze sample in container if collected outside of Special shipped at 2-8 C if Chemistry operating hours. sent within 3 days of collection*

PTH Selective Venous Series PTHSVB 1 SST Gold top 1.0 mL serum. Send on ice pack. Copy of requisition for Chem. Mon – SPH Special Chemistry Fri Indicate vein Sites at Order Entry Modifier field and result PTSITE (second screen) with free text: e.g. PTSITE = ;High Left Internal Jugular

Parathyroid Hormone Related NCODE collect on ice- Must be fasting. Minimum 0.25 mL EDTA plasma spin down in a 60 Days Y Specimen Process Center Peptide (PTHRP) cooled 3 mL EDTA chilled centrifuge. Send frozen on dry ice. Approval required for Mayo Medical Laboratories (PTH Related Peptide) all patients. Ensure Bill 73 is completed and copy of requisition 3050 Superior Drive NW SEE Supervisor or for Sendout bench. Rochester, MN 55901 Mayo Test ID:PTHRP Biochemist for 1-800-533-1710 instructions. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Additional information on Mayo website: http://www.mayomedicallaboratories.com/test- catalog/Specimen/81774

Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Paretial-Cell-Antibody APCA 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab (see AMA + ASMA)

Paroxetine (Paxil) PARXB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Parvovirus B19 IgG PARVGB 5 mL SST Gold top Refrigerate. BC Centre For Disease Control - Fifth Disease C/O PHSA Laboratories – Lane Level Laboratory

Parvovirus B19 IgM and IgG PARVP 5 mL SST Gold top Refrigerate. . BC Centre For Disease Control - Fifth Disease C/O PHSA Laboratories – Lane Level Laboratory

Parvovirus B19 PCR PVB19B 1 RED top **Do not open tube and do not spin** Send sample to BCCH Children’s Hospital Lab Human Parvovirus B19 NAT within 48 hrs. Send copy of requisition with transport batch.

Paternity-Testing No longer available – updated 04/11/08 kl

Paul-Bunnell No longer available

PAX gene RNA Refer patient to Children’s Hospital

PCB or Polychlorinated Bi Not routinely available. For industrial claim consult physician at Ohenols WCB.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 1 RED top Phencyclidine or PCP (Angel PCPSMB 2-4 mL serum. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Dust) – Serum (Qualitative) (PCP Serum)

PCP (Angel Dust) – Urine PCPSMB 50 mL urine Refrigerate, send on ice pack. Y Provincial Toxicology Center Sunquest Order Entry: remove “R” container and add “UR” container. PCR For AML-M4 For Inversion Any PCR done at BCCA, phone for instruction and specimen Y BCCA 16 requirement.

PCR for non-blood specimens PVPCR Fluid Consult SPH Virology for specimen handling SPH Virology (fluids, CSF, urine, swabs) CSF includes: Urine Swabs Adenovirus PCR, BK virus PCR, CMV PCR, EBV PCR, Enterovirus PCR, HIV Viral Load HSV 1 & 2 PCR, JC Virus PCR, VZV PCR. 1 RED top Pentazocine (Talwin) PENTZB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Pentazocine 1 RED top Pentobarbital (Nembutal) PNTBB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Pentobarbital Pentothenic Acid (Vitamin B5) Not available 1 RED top Pericyazine PERICB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Pericyazine Perphenazine (Trilafon) PRPHB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Perphenazine Pertussis NAT CSBP Special Kit Special Pertussis Kit Obtained From The Lab (SPH Central Children’s Hospital Microbiology Processing Microbiology cupboard).

Division of Pre and Post Examination, Page 206 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

PERTUSSIS SEROLOGY PERSSB 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control ***Not Available At BCCDC*** Top tube C/O PHSA Laboratories – Lane Result with “PERSER” – Pertussis Serology Testing Is No Longer Level Laboratory Available. Pertussis Culture/PCR Is The Recommended Test For Diagnosis Of Pertussis. Specimens Must Be Collected On A Special Pertussis Kit Obtained From The Lab (SPH Central Processing Microbiology cupboard).

PG (P-Glycerol) + (LS) Unavailable. Revised Aug01/03. pH, Fluid FPH Syringe 0.5 mL fluid. 24 Hour SPH Chem FTYPE: PLEUR; DIA; PERIT; BAL; PCF Synovial fluid OSM not available. pH, Stool (stool pH) STPH Stool Fresh random stool specimen, freeze within 1 hour of collection. 24 Hour Y Children’s Hospital Must arrive at Children’s before noon the day after collection. 1 RED top Phenacetin PHACB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Phenacetin 1 RED top Phendimetrazine SPQ 2-4 mL serum or urine. Refrigerate. Send on ice pack. Sunquest Y Provincial Toxicology Center Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Phendimetrazine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 207 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Pheniramine (Dristan) PHRMB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 208 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Phenothiazines Qualitative UDRG 50 mL urine Refrigerate urine. Send on ice pack. Y VGH Lab 1 RED top Phentermine (Ionamin) PHTMB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 209 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Phentemine 1 RED top Phenylbutazone (Butazolidin) PHBZB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Phenylbutazone Phenylpropanolamine PHPLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Phenylpropanolamine 0.5 mL Lithium heparin plasma. Collect just prior to next dose – Phenytoin Level (Dilantin, PHEN 1 PST 24 Hour SPH Chem confirm last dose time with patient. Diphenylhydantoin) Sunquest Last Dose format: PHNDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE PHNTLD: ;HH:MM e.g. ;13:50 or UNAVOE Phensuximide PHNS 2-4 mL RED top Refrigerate Serum. Y Provincial Toxicology Center ***NOT AVAILABLE 06/2014 serum

Phosphate PO4 1 PST 0.5 mL plasma, fasting preferred. 24 Hour SPH Chem Division of Pre and Post Examination, Page 210 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Phosphate, fluid FPO4 1 PST Light green 0.5 mL fluid. 24 Hour VGH top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid PO4 not available. preservative container

Phosphatidylserine Antibodies, NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler IgG, IgM, IgA send frozen on dry ice. Mitogen Advanced Diagnostics Lab 1 SST Gold top HMRB 431 – 3330 Hospital Dr. NW (anti-phosphatidylserine Ab) Use Mitogen Diagnostics Laboratory Requisition (in the Calgary, AB, T2N 4N1 Requisitions Folder of the Accessioning Folder). Phone 403-220-4582 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Phosphoethanolamine PAMINO 6 mL DRK GREEN Prior consultation required. Minimum 1.0 mL blood collected on Y Children’s Hospital – Biochemical (Plasma) Li or Na heparin ice. 12 hours or overnight fast for adults and older babies. Genetics Lab 3-4 hours fast or collect before the next feed for small babies or Room 2F22 children under 1 year. 604-875-2307 Centrifuge blood within 1 hr of collection and separate plasma. Ship frozen plasma within 3 days of collection on dry. See Children’s Handbook for additional information. Copy of requisition for send out.

Phosphoethanolamine (Urine) RUAM 24 HR urine Freeze during collection. To be ordered with plasma Y Children’s Hospital – Biochemical Add CRU, phosphoethanolamine. Patient to be fasting. Genetics Lab UTIM Room 2F22 Must mix urine with magnetic stir bar for 5 minutes before removing aliquot for creatinine. Ship entire remaining 604-875-2307 specimen frozen, do not split with other testing.

Phosphoethanolamine Or RUAM Minimum 15 mL Component of Urine Amino Acids. Note: Must mix urine with Y Children’s Hospital – Biochemical Hypophosphatasia add CRR random urine magnetic stir bar for 5 minutes before removing aliquot for Genetics Lab creatinine. Ship entire specimen frozen, do not split with other Room 2F22 testing. Copy of requisition required for send out 604-875-2307

Division of Pre and Post Examination, Page 211 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Phospholipase A2 Receptor NCODE 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler (Anti-Phospholipase A2 send frozen on dry ice. Mitogen Advanced Diagnostics Lab 1 SST Gold top receptor, anti-PLA2R) HMRB 431 – 3330 Hospital Dr. NW Use Mitogen Diagnostics Laboratory Requisition (in the Calgary, AB, T2N 4N1 Requisitions Folder of the Accessioning Folder). Phone 403-220-4582 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Refrigerate during collection. Measure 24 hour volume. Aliquot 3 Phosphorus, 24 hour urine PO4U 24 hour urine, no Mon – SPH Chem mL for urine creatinine, centrifuge and aliquot supernatant to 12 preservative Fri Add CRU, x75 plastic tube. Aliquot approximately 100 mL from well mixed 24 (Acid container UTIM hour collection and adjust the pH of aliquot to pH 2-3 with 6 M HCl acceptable) (do not send whole collection) for heating at SPH. Referring sites need to include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack. Phosphorus, random urine PO4RU Random urine Order code includes urine creatinine and ratio. 3 mL random 24 Hour SPH Chem urine aliquot, centrifuge and aliquot supernatant to 12 x75 plastic tube. Refrigerate and send on ice pack

Phosphorus, Stool (stool STPHOS Stool in non Centrifuge liquid stool, 0.25mL supernatant required. 24 Hour Phosphorus) preservative container

Phytosterol NCODE Red Cells & Freeze cells and plasma. Send on dry ice. Copy of requisition for Y Dr. Howard Parsons plasma send out. Put Fax Result to SPH Lab request sticker on University Of Calgary requisition or on Sunset Printout. Dept. Of Pediatrics Room 1713 Health Sciences Center 3330 Hospital Dr., N.W. Calgary, Canada T2n 4n1

Pimozide Unavailable

Pipecolic Acid PICA 3 mL EDTA Fasting not required. Minimum sample 1 ml EDTA plasma. Children’s Hospital Pyridoxine-Responsive Seizure Spin down within 1 hour of collection, freeze, send frozen. Screen

Piroxicam SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Piroxicam SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE Division of Pre and Post Examination, Page 212 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Plasminogen Activator Inhibitor Unavailable See Hematology prior to collecting. Copy of requisition to Hem. Y Hospitals In-Common Laboratory Plasminogen NCODE 1 Citrate tube Minimum 0.5 mL Citrated Plasma. Freeze. Send frozen on dry 7 days Y Inc. ice. Copy of requisition for send out. 57 Gervais Dr. Additional information on HICL website: North York, ON M3C 1Z2 http://www.HICL.on.ca/search_tcna.asp?tcstring=plas Phone 416-391-1499 Ext.248 Put Fax Result to SPH Lab request sticker on requisition or on Fax Phone 416-385-1957 Sunset Printout.

Platelet Aggregation PLAG 8 Citrate tubes. See Hematology prior to collecting. Do not centrifuge. TAPE ON Pre- Y SPH Do not place on STOPPER. Copy of requisition to Hem. Deliver immediately to booking ice block. Hem. required

Platelet Antibody Investigation PLAB ADULT:7Ml clotted Do not spin. Send at room temperature to CBS within 24h of blood & 5X7Ml collection. Completed platelet antibody investigation request form

(see Anti-Platelet Antibody) EDTA. must accompany specimen. Blank forms found in forms/reqs section. CHILDREN UNDER 12Y: 1X7 Not available as of Oct/03. mL whole blood + 2X7Ml EDTA.

Platelet Function Analysis PFAC 3 BLUE CITRATE Collect only Monday To Friday between 0800 – 1400. Tape on Daily SPH (PFA –100) TEST NO stopper, do not spin and deliver to Hematology immediately. before

LONGER AVAILABLE 06/2014 Stable for 4 hours only. 14:00

Platelet Factor III No longer available. (Hardisty Test)

Platelets – Morphology PLTM 1 LAV 24 Hour SPH Hem add CBC

Platelet Neutralization SEE INHIBITORS Internal request by Hematopathologist only. SPH

PMMA SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; PMMA SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 213 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Division of Pre and Post Examination, Page 214 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Pneumococcal Vaccine PNEUPR 1 SST Gold top 2 to 4 mL serum, minimum 0.4 mL. Refrigerate serum (or freeze) Specimen Process Center Antibody Response (Pre) stable for 21 days only. Mayo Medical Laboratories *1 RED top 3050 Superior Drive NW Mayo Test ID: PN23 acceptable* Patient must pay $215.78 USD ($65.78 + $150.00 for S&H) for Rochester, MN 55901 testing if there is no written letter of MSP preapproval. 1-800-533-1710 1. Patient must sign Bill 73 form.

2. Complete and print OP Fee schedule. 3. Ask patient to pay at Cashier and make 2 copies of payment receipt, one stapled with copy of requisition for Sendout Tech, the second stapled to original requisition. SPH Sendout: Do not wait for Post Vaccine specimen before sending. Order Test in MayoLink and send with MayoLink requisition.

Pneumococcal Vaccine PNEUPO 1 SST Gold top 2 mL serum, minimum 0.4 mL. Refrigerate serum (or freeze) Specimen Process Center Antibody Response (Post stable for 21 days only. Mayo Medical Laboratories *1 RED top Vaccine) 3050 Superior Drive NW acceptable* Patient must pay $215.78 USD ($65.78 + $150.00 for S&H) for Rochester, MN 55901 Mayo Test ID: PN23 testing if there is no written letter of MSP preapproval. 1-800-533-1710 1. Patient must sign Bill 73 form.

2. Complete and print OP Fee schedule. 3. Ask patient to pay at Cashier and make 2 copies of payment receipt, one stapled with copy of requisition for Sendout Tech, the second stapled to original requisition. SPH Sendout: Order Test in MayoLink and send with MayoLink requisition. Polyols PLYO Random urine, no 10 mL preferred (minimum 2.0 mL) random urine. Freeze and Taldo Children’s Hospital preservative send frozen. Transaldolase deficiency PPP deficiency Pentose phosphate pathway RPI deficiency Ribose-5-phosphate deficiency

Division of Pre and Post Examination, Page 215 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory Porphobilinogen Deaminase NCODE + 6 mL DRK GRN Pre-approval is required. 10 days Y Inc. HCT top Na Heparin Order HCT and send result with specimen 57 Gervais Dr. **gel separator Keep in fridge. Do not centrifuge. Do not freeze. Protect from North York, ON M3C 1Z2 tubes are light. Copy of requisition for send out. Put Fax Result to SPH unacceptable** Lab request sticker on requisition or on Sunset Printout. Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 3 mL EDTA Additional information on HICL website: http://www.HICL.on.ca/search_tcna.asp?Tcstring=PBGD 12 mL first Porphobilinogen, Random PBGR Freshly voided first morning urine specimen acceptable up to 4 Y VGH Lab morning urine Urine hours old. Specimen must be protected from light. Adjust pH to specimen in 7.0 – 8.0 with sodium carbonate. orange top container, protect from light

Consultation with Medical/Clinical Biochemist recommended— Porphobilinogen Quantitative PBGU 24 HR urine in 5 Y VGH Lab for both cutaneous and acute porphyria investigations a random grams Anhydrous Add CRU, urine is used as the first-line test. Sodium Carbonate UTIM protect from light. Specimen must be protected from light, adjust pH to 7-8. Send 50 mL aliquot, on ice pack.

Porphyria Variegata 2 X 6 mL EDTA Prior consultation required. Send at room temp along with Y Children’s Hospital – Molecular whole blood completed Molecular Diagnostic Laboratory requisition. Blank reqs Diagnostic Laboratory Rm 2J40 – NO LONGER AVAILABLE in forms/reqs section 11/2014 4480 Oak St. PORR Random first Consultation with Medical/Clinical Biochemist recommended— Porphyrin (Urine) Copro And Y VGH Lab morning urine for initial investigations of cutaneous porphyria a random Uro Porphyrin specimen specimen in usually preferred.

PORU 24 h urine Protect from light during collection and storage. Adjust pH to 7-8; collected in 5 freeze; ship on ice pack. Add CRU, grams sodium UTIM bicarbonate or Random – send 10 mL sodium carbonate 24 h – send 50 mL Porphyrin (Stool) PSCNF Random or 24 hr Random collection in a stool jar. Protect from light. Freeze Y VGH: Random Specimen collection immediately. Test to be performed within 1-2 days of collection. -Updated: Jan/05 Quantitative: pre-weighed tin refrigerates. VGH lab assays only LIFELAB: 24HR Specimen random specimens.

Division of Pre and Post Examination, Page 216 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Porphyrins (Plasma) PPOR 3 mL EDTA Remove plasma from cells immediately after spinning. Y VGH Lab plasma Protect from light and refrigerate. Add HCT

Posaconazole POSAB 1 EDTA 1 mL EDTA plasma Centrifuge ASAP. Freeze plasma. Date and Weekly – SPH Lab time of last dose and dosage of posaconazole preferred but Wed. not mandatory.

Potassium, 24 hour urine KU 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem preservative hour collection (do not send whole collection). Referring sites Fri Add CRU, (Unsuitable if need to include 24 hour volume, patient’s height and weight on UTIM collected in Acid) requisition. Refrigerate and send on ice pack.

Potassium, random urine KR Random urine 3 mL random urine, refrigerate. 24 Hour SPH Chem

Potassium, stool (Stool STK Stool in non Liquid specimen required. Test within 4 hours if stored at room 24 Hour SPH Chem potassium) preservative temp., and within 24 hours if refrigerated. 0.5mL supernatant container required, more if Millipore filtration is required. BC Centre For Disease Control Powassan Virus Serology PVA 5 mL SST Gold top Refrigerate C/O PHSA Laboratories – Lane Level Laboratory Prazepam SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Prazepam SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Pre-Admit Group & Screen PGRS 2 x 6 mL ETDA Signed Type and Screen requisition required for testing. 2-4 SPH Transfusion Medicine (Blood (Type & Screen, PHC) hours Bank) Mon – Fri

Pre Albumin PALB 1 SST Gold top 0.5 mL serum, refrigerate. Daily SPH Chem (Transthyretin)

Prednisone PRDNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 217 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Pregnanetriol Not available suggest 17 hydroxyprogesterone Y BC Centre For Disease Control Pre Heart Transplant PHEART 3 x 5 mL SST Gold Refrigerate. Send on ice pack. C/O PHSA Laboratories – Lane EBGSB, RUBEB, TPE, top Level Laboratory TOXGSB, HSVIGB, MIGB, MUIGB, PHBSAB, PHCV, SPH Virology HIVCA, VZIGB, , PCMVG, PHBSAG, PHBCAB VZIGB to BCCDC Pre Renal Transplant PRENAL 2 x 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control EBGSB = Anti-EBV IgG top and C/O PHSA Laboratories – Lane Add TPE = Syphilis Level Laboratory MISPCR Add MISPCR (if West Nile virus ordered): HSVIGB = Anti-HSV IgG (if West 6 mL LAV top West SPEC5=PLS MIGB = Measles IgG Nile Virus Nile Virus SPVIR2=;West Nile virus, transplant MUIGB = Mumps IgG ordered: RUBEB = Rubella IgG SPVIR1=; VZIGB = Anti-VZV IgG WEST

NILE PCMVG = Anti-CMV IgG VIRUS, SPH Virology PHBSAG = HBSAG TRANSPL PHBSAB = Anti-HBS ANT) PHBCAB = Anti-HBC Total PHCV = Anti-HCV HIVCA = Anti HIV Ag/Ab HTLVB = Anti-HTLV I/II

Required requisition (and copy for Sendout): Prenatal Genetic Prenatal Biochemical Screen PREAMN Amniotic fluid Children’s Hospital Screening Laboratory Requisition Amniotic Fluid SPH Accessioning Tech: place one Child label on requisition after PDEC in SMART. Send requisition with transport batch. Division of Pre and Post Examination, Page 218 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Prenatal Biochemical Screen 1 RED top A completed Prenatal Genetic Screening Laboratory Children’s Hospital Requisition must be provided by the patient or physician; copy Serum Integrated Prenatal PREBIO *5mL SST Gold top for Sendout. (Sample of req. link): Screen Part 1 acceptable* http://www.perinatalservicesbc.ca/NR/rdonlyres/84E727DE-

Integrated screen Part 2 QUAD: B3DD-49AE-8BDE-59312BE2B16A/0/LabReqFillable.pdf includes: AFP,UE3,hCG, or Inhibin A http://www.perinatalservicesbc.ca/ScreeningPrograms/Prenatal Alpha Fetoprotein – Maternal GeneticScreening/healthcare-providers/requisition-forms- Serum reports.htm

Maternal Alpha Fetal Protein SPH Accessioning Tech: Triple Marker Screen (Serum) 1. Process PDEC in Sunquest SMART. 2. Aliquot 3.0 mL serum from Red top tube. Freeze (Includes: Serum Integrated specimen. Prenatal Screen SIPS Or 3. Place one Child label on requisition. Intergrated Prenatal Screen 4. Send on dry ice preferred. Send copy of requisition IPS) with Child CID label with Transport Batch.

Division of Pre and Post Examination, Page 219 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Prenatal Guidelines: PRSB SPECIMEN Tests performed CBS (Perinatal Screen Request, REQUIREMENTS Note: CBS Requires CBS Prenatal Group and Screen) Requisition With Due Date, Hospital Pregnancy 6 mL EDTA ·ABO/RH and Antibody screen For Delivery, And Requesting

Prenatal Patients Physician Information On This Req.

6 mL EDTA ·ABO/RH Fathers – Only Fathers That ·Red Cell Phenotype Have Been Specifically Requested By The Red Cross Will Be ·ABO/RH Tested. ·Red Cell Phenotype 6 mL EDTA ·DAT Cords – Only Cords That Have Been Specifically Requested By The ·ABO/RH and Antibody screen Red Cross Will Be Tested. ·ABO/RH and Antibody screen Scheduled Therapeutic Abortions 6 mL EDTA *Scheduled Patients ABO/RH

Infertility 6 mL EDTA

Prospective Prenatal All specimens store in fridge Patients 6 mL EDTA Prospective Fathers

EDTA- Purple/Lavender Top *Note: Due To High Volume Automation, Stat Testing Is Unavailable At Our Facility. 2 x 5 mL SST Gold Prenatal Screen Guidelines: PRENAT Refrigerate. Send on ice pack. BC Centre For Disease Control top HBVP (Hepatitis B Surface Must send PSTORE barcode to BCCDC (with/without specimen). C/O PHSA Laboratories – Lane Ag) STAT requests after hours must be approved by BCCDC Level Laboratory HIVCC (Anti HIV Ag/Ab Medical Microbiologist @ 604-661-7033 Combo) RUBEB (Rubella IgG ) TPE (Syphilis – T. Pallidum Screening or RPR))

Division of Pre and Post Examination, Page 220 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Prilocaine (Citanest) PRILB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Procainamide (Pronestyl; N- PROCB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center Acetyl Procainamide) requisition for send out.

Division of Pre and Post Examination, Page 221 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Procyclidine (Kemadrin) PRCLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 222 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Prolactin PROL 1 SST Gold top 0.5 mL serum in False Bottom or 13 x 75 Polypropylene tube. Mon & SPH Special Chemistry Send with ice pack. Do NOT order together with MPROLB Thurs

Proliferating Cell Nuclear NCODE 1 SST Gold top 2mL serum. Freeze and send on ice pack. Stable for 1 week Y Quest Diagnostics Nichols Institute Antigen Antibodies ambient, 2 weeks refrigerated. 33608 Ortega Highway Mandatory MSP pre-approval required for Outpatients San Juan, Capistrano, (request to be done by patient Dr.). For Outpatients, do not CA 92675 collect without MSP preapproval if test is ordered alone. 1(800) 553-5445 Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker

Promazine SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Promazine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 223 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Promethazine PMTHB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 224 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Propoxyphene 1 RED top Propranolol (Inderal) PROPB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Proprandol Prostatic Specific Antigen, Free PSAFB 1 SST Gold top 1 mL serum. Freeze Serum. Send on ice pack. BC Centre For Disease Control (Includes PSA Total, PSA Free, C/O PHSA Laboratories – Lane PSA Free Ratio) Level Laboratory

Prostatic Specific Antigen PSAP 1 SST Gold top 1 mL serum in False Bottom or 13 x 75 Polypropylene tube, Mon, SPH Special Chemistry Screen freeze serum. Send with ice pack. Tues, Wed, Fri 1 mL serum. Pre-pay or collect blood then give copy of request to Prostatic Specific Antigen (Self PSASP Mon, SPH Special Chemistry 1 SST Gold top billing. Store in fridge. Pay) Tues, Wed, Fri

Protein C PCCA 2 CIT Full citrate draw. Determine if patient is on oral anticoagulant. 1-2 times Y SPH Hem (Antigen and Activity) Specimen must be centrifuged within 2 hours of collection.1.5 per week mL plasma double spun, aliquot in 2 polypropylene tubes. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy of requisition for Special Coag. For Babies, ask for Hematopathologist approval prior to collecting 1 x 1.8mL citrate by venous; ask Dr to collect if Phlebotomist is not certified to perform venous collection.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Protein C Activation Resistance No longer available To (Resistance To Activated Protein C)

Protein S SACT 2 CIT Full citrate draw. Determine if patient is on oral anticoagulant. 1-2 times Y SPH Hem (Total and Free, Specimen must be centrifuged within 2 hours of collection. 1.5 per week Protein S Ag Free) mL plasma double spun, aliquot in 2 polypropylene tubes. Freeze at -20°C, refer in sample must arrive at SPH frozen. Copy of requisition for Special Coag.

PRU 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem preservative hour collection (do not send whole collection). Referring sites Fri Protein, 24 hour urine Add CRU, (unsuitable if need to include 24 hour volume, patient’s height and weight on UTIM collected in Acid) requisition. Refrigerate and send on ice pack. Protein Creatinine ratio PRCRR Random urine Order code includes urine creatinine and ratio. 3 mL random Daily SPH Chem (random urine protein not urine, centrifuge and aliquot supernatant to 12 x75 plastic tube. orderable) Refrigerate, send on ice pack.

FTP 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF FTYPE Protein, Fluid RED top or non Synovial Fluid TP not available. preservative container

Protein (Total) TP 1 SST Gold top 0.5 mL serum 24 Hour SPH Chem

Prothrombin Gene Mutation TDNA 6 mL EDTA Refrigerate. Send whole blood on ice pack. Always perform with N - if ordered VGH Special Coag Factor V Leiden. by Drs.: (620210a) (DO NOT OPEN 875-4111 L66400 Ezzat, OR Foltz CENTRIFUGE) S. Jackson, C.Leger, H.Leitch, A. Rahmani Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Protoporphyrins – Red Blood FEP 1 LAV EDTA tube Do not centrifuge. Protect from light, wrap in tin foil. Send Y VGH Lab Cell hematocrit result. *wrapped in foil*

Protriptyline PRTRB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center (Tricyclic Antidepressants) requisition for send out. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Protriptyline Proviral DNA HIVPB EDTA whole blood Must receive within 3 days of collection. BC Centre For Disease Control Not available. Not Available: order HIVTQ if patient is not from Centre For C/O PHSA Laboratories – Lane Excellence.. Level Laboratory

Pseudocholinesterase PSDCS 1 RED top 2-4 mL RED top serum. Send frozen. Put Fax Result to SPH Royal Columbian Hosp Lab request sticker on requisition or on Sunset Printout. 1 RED top Pseudoephedrine PSEUB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Pseudoephedrine 1 RED top Psilocin SPQ 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; PSILOCIN SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

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Psittacosis (Chlamydia) CHLGRP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Serology (IgG, IgM) C/O PHSA Laboratories – Lane Trachomatis Level Laboratory Chlamydia Pneumoniae Chlamydia Psittaci Purines and Pyrimidines PPGC 10 mL Random Minimum 2 mL. Freeze urine and send frozen. Y Children’s & Women’s Health (Creatine, GAA, GMAT, urine in sterile Centre of British Columbia – Add AGAT, Guanidinoacetate, container Laboratory CRR Adenine, Hypoxanthine, 4500 Oak Street Xanthine, Vancouver, BC Deoxyandenosine, V6H 3N1 Deoxyinosine, Specimen Receiving, 2J20 Deoxyguanosine, Adenosine, Indosine, Guanosine, Succinyladenosine, Thymine, NCB-ALA Aminolevulinic Acid, Deoxyuridine, Guanine) Pyrilamine SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Pyrilamine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Pyrimethamine Not available

Pyroxide or Pyroxin Pyridoxine No longer available.

Pyruvate, Blood PYRU PYRU tubes Refer patient to Children’s Hospital laboratory – if not possible (i.e. Y Children’s Hospital (obtain from inpatients), Prior consultation with Children’s Hospital is Order BCCH) required. Obtain two special perchloric acid (PYRU) tubes Plasma from CW Biochemical Genetics Lab. Use syringe to draw lactate at blood without tourniquet. Add exactly 1 ml whole blood to same each special PYRU tube. Two tubes are required. Mix well. time. Transport immediately to lab on ice. Ensure lactate is ordered. Please refer to Children’s eLab Handbook for complete instruction. Copy of requisition for send out. Children’s Hospital Pyruvate Kinase Assay PKSB 6.0 mL EDTA Consult with Hematopathologist prior to collection. Copy of Y Do NOT order PKAB code. whole blood requisition to Hem. And Send out. Store and send at 4°C. Do 604-875-2345 x7502 not freeze. Blood transfusions within the last 3 months invalidates test results. Analysis must be performed within 5 days of collection. Children’s will forward PKAB to HICL if PKSB screen results indicate PKAB is required.

Pyruvate Kinase Screen PKSB Babies: 1.0 mL Consult with Hematopathologist prior to collection. Optimal 3.0 2-5 days Y Childlren’s Hospital (Pyruvate Spot Test) micro EDTA mL, minimum 1.0 mL whole blood. Refrigerate specimen if testing 604-875-2345 x7502 Adults: 3mL delayed. Send on wet ice (not Frozen) ice pack. Sample must be EDTA tested within 48 hrs of collection; received at Children’s before noon on Friday.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Quantiferon TB Gold TBQS ***Special Set*** Special Collection Set: “Quantiferon – TB Gold In-Tube” 3 tube BCCDC ZEP Lab (TB Quantiferon Gold) set notes: Contains Lithium (Interferon Gamma Release heparin  Lithium heparin additive in all 3 tubes, so insert the 3 tubes in Assay – IGRA) the correct order of draw. E.g. after light green PST.

 All 3 tubes need to be collected as a set, if one is wasted, all Collection sets 3 are wasted. Fill to black mark on tube (1.0mL ±0.2mL fill). stored in Outpatient Supply 1. Use a 6mL DARK GREEN tube as primer if only TBQS cabinet, next to ordered; tube can be removed once blood flow starts. Viral swabs. 2. Order of draw for set: Gray cap, Red cap, Purple cap. 3. Mix 3 tubes 8 times by turning end over end or gently shake for 5 seconds, avoid frothing. 4. Barcode label Grey (Nil) with 1st CID (bottom barcode), Red (TB Ag) with 2nd CID and Purple (Mitogen) with 3rd CID (top barcode). Keep tubes upright at Room Temp 22°C ± 5°C for up to 16 hours. 5. Send tubes to Microbiology within 16 hours of collection for incubation. Accessioning Tech SMART track to PMC Spot. 6. Microbiology incubates tubes upright at 37°C for 16-24 hours. 7. Microbiology centrifuge tubes at 2000 rcf for 15 minutes 8. Microbiology transfers plasma to cryovial labelled with matching CID and pen marked N on Gray, T on Red, M on Purple. 9. Store plasma samples up to 1 week at 2 – 8°C or -20°C for extended storage (>1 week). 10. Micro give samples, 3 tube set per biohazard bag labelled with “Attention ZEP Lab”, to Accessioning Sendout Tech. 11. Sendout Tech create Transport batch, one batch for all TBQS patients. 12. Samples must arrive at BCCDC before 18:00 Mon – Fri.

Quetiapine (Seroquel) QUETB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Quinine QUNNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Raji Cells (Part Of CIQB) No longer available VGH Lab (see Circulating Immune Complex)

Ramapril SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; RAMAPRIL SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

RAST, Allergy Screen RAST 1 RED top 2-4 mL Red top serum preferred, minimum 0.1 mL serum per Y Children’s Endocrine Lab (RAST-RIA Semi Quantitative allergen. Consult lab for specific allergen groups. Refrigerate and

Measure of Specific IgE send on ice pack if sent same day. Freeze and send on dry ice if Directed Against Selected not sent the same day. Must include Dr’s signature, diagnosis Allergen Groups) and history. (Allergen Specific IgE Copy of requisition for send out. Physician needs to fill out Antibody, Allergy Specific IgE allergen specific IgE antibody form in the forms/req section: antibody ) http://www.elabhandbook.info/PHSA/Files/RequisitionFor ms%2f1_20170424_055445_1_20140605_015854_aller gy%20req%20MAY2014.pdf

Red Cell-Fragility or Osmotic + 2 tubes10 mL Must be booked Mon – SPH Special Hem Incubated Fragility Heparin blood + 3 Fri NOT AVAILABLE. Order mL EDTA (PT + PNHFLO Control)

RBC genotyping AICBSB 6mL EDTA tube Whole blood EDTA unopened. Deliver specimen and copies of all CBS via SPH TM accompanying paperwork directly to Transfusion Medicine. (red cell genotyping)

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

RBC Membrane Flow Analysis EMARBC 1 EDTA Optimal 2 mL; Minimum 1 mL Whole blood Y Specimen Receiving, 2J20. Test must pre-booked with the CW Immunology Laboratory. (HS, Hereditary spherocytosis, Children’s & Women’s Health Centre EMA, eosin 5 maleimide, E5M, Specimen must be received and processed within 48 hours of of British Columbia – Laboratory Osmotic Fragility, OF, RBC collection. Transport in an insulated container on a cold pack. 4500 Oak Street Membrane Protein Analysis, Include a peripheral blood smear with all referred in specimens. Vancouver, BC Band 3 Protein by Flow, EMA V6H 3N1 Flow Immunology)

Remifentanyl SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; REMIFENTANYL SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Renin Aldosterone ratio ALDRB Collect after patient in a seated position for 5-15 minutes. Collect Weekly - SPH Special Chem Lab (Renin Angiotensin ratio) RNN 3 mL EDTA on ice on pre chilled EDTA tube and then placed on ice, centrifuge at result on 4°C ASAP (within 15 minutes). 1.0 mL EDTA plasma store and Tuesday send Frozen (dry ice preferred). Collect on pre chilled EDTA tube and then placed on ice, Renin (Plasma Renin activity) RNN Weekly - SPH Special Chem Lab centrifuge at 4°C ASAP (within 15 minutes). 1.0 mL EDTA plasma result on store and send Frozen (dry ice preferred). Specify posture at Tuesday time of collection: Supine = collect blood after one hour in the prone position. Upright = collect blood after two hours in the upright position 3 mL EDTA on ice (standing). Renin Series: Renin collected at different locations minutes apart. Order Entry: Must use Order Comment field to indicate Specimen Site and also add site information in Modifier field. E.g. Order Comment = Left renal or Right Renal

Division of Pre and Post Examination, Page 233 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Renin Supine RNNSU Collect on pre chilled EDTA tube and then placed on ice, Weekly - SPH Special Chem Lab 3 mL EDTA on ice centrifuge at 4°C ASAP (within 15 minutes). 1.0 mL EDTA plasma result on store and send Frozen (dry ice preferred). Collect blood after one Tuesday hour in the prone position.

Renin Upright RNNUP Collect on pre chilled EDTA tube and then placed on ice, Weekly - SPH Special Chem Lab centrifuge at 4°C ASAP (within 15 minutes). 1.0 mL EDTA plasma result on 3.0 mL EDTA on store and send Frozen (dry ice preferred). Tuesday ice Collect after the patient has been awake ambulating and/or seated in upright posture. The patient should not have been lying down at any time two hours prior to collection.

Reptilase Time No longer available

Resistance to Thyroid Hormone NCODE 6 mL Tall EDTA Minimum 3 mL EDTA whole blood. Do not spin. Store and send Y Quest Diagnostics Nichols Institute (RTH) Mutation Analysis at room temperature. Do NOT freeze. Stability 8 days at room 33608 Ortega Highway temperature. More information: (THR mutational analysis) San Juan Capistrano, CA 92690- http://www.questdiagnostics.com/testcenter/TestDetail.action?tab 6130 Name=OrderingInfo&ntc=16053 1(800) 553-5445 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker BC Centre For Disease Control Respiratory Nucleic Acid Test LUMXR Indicate specimen type at RESOE Flu PCR (SD0120)

Respiratory Syncytial Virus 5 mL SST Gold top Do not freeze. Virology Lab

RET (Proto-Oncogene) NCODE 2 tubes 6 mL Prior consultation required. Send at room temp along with Y BCCA – Molecular Genetics EDTA whole blood completed form found in between this sleeve. Copy of requisition Laboratory 3rd – Fl 600 Multiple Endocrine Neoplasia th for send out. West 10 Avenue Type 2 If cancer related send to BCCA Children’s Hospital – Molecular RET Mutation Diagnostic Laboratory Rm 2J40 – If non-cancer related send to Children’s Hospital (MEN-2) 4480 Oak St.

Reticulocyte Count RET 1 LAV Minimum 1.0 mL collection using 3 mL EDTA. 0.25 mL for micro 24 Hour SPH Hem + CBC EDTA. Sample stable 3 days.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Reticulocyte Count, manual RETM 1 LAV Minimum 1.0 mL collection using 3 mL EDTA. 0.25 mL for micro 24 Hour SPH Hem + CBC EDTA. Sample stable 3 days.

Rheumatoid Factor RF 0.5 mL Lithium Refrigerate. Send on ice pack. Sample stable for 1 week at 2- VGH Lab heparin plasma 8°C. preferred, Serum Do not need to be decanted. acceptable Rheumatoid Factor, Fluid RF add Fluid specimen Refrigerate. Send on ice pack. VGH Lab FTYPE

Rickettsia Rickettsii Antibody RICKRB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Rickettsia Serology RICKRB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Rocky Mountain Spotted Level Laboratory Fever)

Rickettsia Species Serology RICKSP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Weil-Felix, Rickettsia Serology, C/O PHSA Laboratories – Lane Scrub Typhus Serology Level Laboratory

Rickettsia Typhi Serology RICKTY 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Endemic Typhus Fever C/O PHSA Laboratories – Lane Serology) Level Laboratory

Risperidone (Respiridal) RISPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Ristocetin Induced Platelet RIPAB 5 Citrate tubes See Special Hematology (Special Coagulation) before Pre- Y SPH-Special Hematology Aggregation collecting.Put Tape ton ube top. Do not centerfuge samples. Do booking (full draw) not put on ice block. Deliver samples directly to Special required Hematology with a copy of the requisition.

Ritalin (Methylphenidate) MTPDB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Ritilinic Acid SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; Ritilinic acid SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Rivotril CLNZB (see Clonazepam) 1 SST Gold top RNP (Anti-RNP, anti-ribonucleic ANAEB 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab protein) days @ 4°C. 1 SST Gold top Ro La antibody (ENA Ab) ANAEB 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab days @ 4°C.

Rohypnol (Flunitrazepam) FLNPAM 1 RED top 2 – 4 mL serum from RED top. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Copy of requisition for send out. Collect blood within 4 to 5 hours of ingestion. If greater than 5 hours, collects urine specimens. Rohypnol usually undetectable in the urine by 24 hours.

Ross River Virus Serology RRV 5 mL SST Gold top Refrigerate. Send on ice pack BC Centre For Disease Control (BCCDC Sendout)

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Rotavirus Stool sample. Latex agglutination. Do not freeze. Indicate specific test. Send to Virology Lab Faeces in plain Prov Lab. container

Rubella – IgG Serology RUBEB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - German Measles C/O PHSA Laboratories – Lane Level Laboratory

Rubella – IgM And IgG RUBP 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Serology C/O PHSA Laboratories – Lane - German Measles Level Laboratory

Russell Viper Venom (Inhibitor) DRVTB 4 Citrate tubes. Full citrate draw. Determine if patient is on oral anticoagulant. 1-2 times SPH Special Coag. (Dilute Russell Viper Venom Specimen must be centrifuged within 2 hours of collection. per week Time, Lupus Anticoagulant, Anti Minimum 3.0 mL plasma double spun, aliquot in polypropylene Phospholipid Antibody, Anti tube. Freeze at -20, refer in sample must arrive at SPH frozen. Phospholipid Screen) Copy requisition for Special Coag.

Salbutamol SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; SALBUTAMOL SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Salicylamide SACTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

San Filippo A WBC NCODE 3 mL DRK GRN **Send patient to BCCH for collection if possible** Y Children’s Hospital – Biochemical top Na Heparin Genetics Lab (Heparan Sulphamidase) Prior consultation required. Contact CW Biochemical Genetics and Li heparin are Room 2F22 Laboratory at (604) 875-2307 for approval. acceptable 604-875-2307 Sample must arrive at BCCH before 1200. Do not collect after **no gel seperator 0900, Monday – Friday. Sendout person must obtain approval tubes** STAT and send by cab. Put CW Biochemical Gentics lab address sticker on TDG container. Alert Children’s when sending specimen. 3 ml whole blood minimum. Blood should be kept at room temp. Copy of requisition for send out.

San Filippo C WBC NCODE 3 mL DRK GRN **Send patient to BCCH for collection if possible** Y Children’s Hospital – Biochemical top Na Heparin Genetics Lab (Mucopolysaccharidosis Type Prior consultation required. Contact CW Biochemical Genetics and Li heparin are Room 2F22 III, Acetyl CoA:Alpha- Laboratory at (604) 875-2307 for approval. acceptable Glucosaminide N-Acetyl) 604-875-2307 Sample must arrive at BCCH before 1200. Do not collect after **no gel seperator 0900, Monday – Friday. Sendout person must obtain approval tubes** STAT and send by cab. Put CW Biochemical Gentics lab address sticker on TDG container. Alert Children’s when sending specimen. 3 ml whole blood minimum. Blood should be kept at room temp. Copy of requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

San Filippo D WBC NCODE 3 mL DRK GRN **Send patient to BCCH for collection if possible** Y Children’s Hospital – Biochemical top Na Heparin Genetics Lab (N-acetylgucosamine-6- Prior consultation required. Contact CW Biochemical Genetics and Li heparin are Room 2F22 sulphatase) Laboratory at (604) 875-2307 for approval. acceptable 604-875-2307 Sample must arrive at BCCH before 1200. Do not collect after **no gel seperator 0900, Monday – Friday. Sendout person must obtain approval tubes** STAT and send by cab. Put CW Biochemical Gentics lab address sticker on TDG container. Alert Children’s when sending specimen. 3 ml whole blood minimum. Blood should be kept at room temp. Copy of requisition for send out. BC Centre For Disease Control St. Louis Encephalitis Serology SLE – 5 mL SST Gold top Refrigerate. Send on ice pack. consult Note: not to be confused with SLE acronyms on requisitions, Dr supervisor must specify St Louis Encephalitis. before

ordering BC Centre For Disease Control St. Louis Encephalitis Hi SLEHI 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control SARS NCODE 5 mL SST Gold top Refrigerate. Send on ice pack. (SARS Is Not Performed AT Manual Requisition BCCDC. Any Request Received Would Require Program Head And Section Head Consult) Schillings Test Patient’s physician must make arrangements with the Nuclear Nuclear Medicine At SPH Med dept at SPH prior to patient having test.

Schistosomiasis Serology SCHISB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Scleroderma Antibody (SCL- ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab 70) days @ 4°C.

Scrub Typhus Serology SCRUB Indicate specimen Refrigerate. Send on ice pack BC Centre For Disease Control (Orientia Tsutsugamushi type at RESOE Serology)

Secobarbital (Seconal) SBARBB 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Selenium SELS 1 NAVY BLUE Centrifuge x2 Y Children’s Hospital Lab Metal free 1st pour into navy vacutainer tube & centrifuge nd Specimen Receiving 2J20. Vacutainer tube 2 pour into: falcon polypropylene tubes and freeze ASAP. 875 – 7520 Plasma from Na heparin navy blue top trace metal tube also acceptable. 1.0mL minimum Serum or heparinised plasma Store at 4 °C and transport on ice. If not sending within the week, freeze and transport frozen on dry ice to Specimen Receiving 2J20. See Children’s trace elements collection: http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_201 40207_113746_Blood%20Collection%20for%20Trace%20Elemen ts%20rev%20Dec%202013.doc

Selenium (24 Hour Urine) Collected with 15 Refrigerate and send CRR result. Y Children’s Hospital Lab mL 6mol/L HCL in Indicate if for trace element analysis or lead chelation test. Copy of NOT AVAILABLE. Order acid washed requisition for send out. . “ Serum Selenium SELS container

Semen Count Contact Lifelab or BCBIO for additional information. Do not Lifelab collect on site.

Semen Morphology Refer patient to Lifelab Lifelab Lab

Sensorineural Hearing Loss MDT 6 mL EDTA Min. 2.0 mL EDTA. Ship at room temperature. Children’s Hospital (GJB2/6) (do not order DFN Send copy of requisition with transport batch. 4500 Oak Street code) Ordering Dr must complete Molecular Genetics Laboratory C&W Vancouver, BC (Connexin 26; Connexin 30; requisition: V6H 3N1 DFNB1; DFNA3; SNHL; Rm:2J20 autosomal recessive deafness) http://www.elabhandbook.info/phsa/Files/RequisitionForms%2f1 _20140722_042203_CWMG_REQ_0000_v4.2_General_Requis ition%20edit.pdf

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Serotonin Release Assay NCODE 1 RED top 2-4 mL RED top serum. Prior consultation required. Coagulation Lab, HSC 3H42 Mcmaster University Allow to clot at room temperature. Centrifuge and transfer Serum 1200 Main Street West into plastic tube. Freeze promptly. Hamilton, Ontario, L8N 3Z5 All samples must accompany patient data form. Hematology will Tel (905) 525-9140 Ext 22414 provide patient data form with the samples, current form: http://fhs.mcmaster.ca/plateletimmunology/documents/patient_re quisition_form_may_2014.pdf Link to McMaster if above link is not working: http://fhs.mcmaster.ca/plateletimmunology/hit.html Send on dry ice to ensure received frozen. Copy of requisition for send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Serotonin Whole Blood NCODE 3ml EDTA whole Prior approval is required. Draw blood in a lavender top (EDTA) Y Specimen Process Center blood tube (s). Transfer specimen to a serotonin tube (Supply T259) Mayo Medical Laboratories Mayo test ID: SERWB containing ascorbic acid and mix well. Immediately freeze 3050 Superior Drive NW (PLUS Special specimen. Ensure Bill 73 is completed and copy of requisition Rochester, MN 55901 tubes with ascorbic for Sendout bench. 1-800-533-1710 acid from Mayo –

must pre-order Must generate Mayo Clinic Order in MayoLink, see Supervisor: supply prior to https://orders.mayomedicallaboratories.com/en/login?SAMLart= collection). NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Additional information on Mayo Website: http://www.mayomedicallaboratories.com/test- catalog/Specimen/84373 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Sertraline (Zoloft) SRTLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 241 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

antibiotic levels. Random samples may also be tested.

Serum Glutamic Do ALT SPH Pyruvic Transaminase

Sex Hormone Binding Globulin SHBGB 1 RED Top 2 mL serum in False Bottom or 13 x 75 Polypropylene tube. Tues & SPH Special Chemistry (Bioavailable Testosterone, Minimum 0.5 mL. Refrigerate. Collect before 10am preferred but Fri Free Testosterone, Free not mandatory. Bioavailable Testosterone alone will not be

Androgen Index ) assayed for male (OK for female). Physician must request Total Testosterone as well. Clarify with physician. If female, log in SHBGB. Copy of requisition to Special Chem for all Self-Pay requests. Send on ice pack within 5 days of collection or send frozen.

Sialic Acid Unavailable

Sickle Cell Screen SIC 1 EDTA tube Copy of requisition to Hem. Mon – SPH Special Hem Same tube as CBC may be used. Fri

Sildenafil SILDEB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 242 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Smith (SM, Anti-Smith, Anti- ANAEB 1 SST Gold top 1 mL serum. Aliquot and Freeze. Send on ice pack. Stability: 3 VGH Lab Sm) days @ 4°C.

Sodium NA 1 PST 0.5 plasma, refrigerate. 24 Hour SPH Chem

Sodium, fluid FNA 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid NA not available. preservative container

Sodium, 24 hour urine NAU 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – SPH Chem preservative. (Acid hour collection (do not send whole collection), centrifuge and Fri Add CRU, container aliquot supernatant to 12 x75 plastic tube. . Referring sites need UTIM acceptable) to include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack.

Sodium, random urine NAR Random urine 3 mL random urine, centrifuge and aliquot supernatant to 12 x75 24 Hour SPH Chem plastic tube, refrigerate.

Division of Pre and Post Examination, Page 243 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sodium, Stool (stool sodium) STNA Stool in non Liquid specimen required. Test within 4 hours if stored at room 24 Hour SPH Chem preservative temp., and within 24 hours if refrigerated. 0.5mL supernatant container required, more if Millipore filtration is required.

Somatomedin-C (IGF1) IGF1 1 SST Gold top Freeze 1.0 mL serum (or heparinised plasma), send on dry ice. Tues/Fri SPH Special Chemistry

Somatostatin NCODE 1 special collection Must be 10 – 12 hour fast and no antacid medication affecting Y Specimen Process Center tube with GI insulin secretion. Ensure Bill 73 is completed and copy of Mayo Medical Laboratories Mayo Test ID: FSOMA preservative requisition for Sendout bench. 3050 Superior Drive NW Rochester, MN 55901 (Special collection Must generate Mayo Clinic Order in MayoLink, see Supervisor: 1-800-533-1710 tubes) https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG Pre-order QTgyREYy%0A collection supply is necessary SEND FROZEN ON DRY ICE. APPROVAL REQUIRED FOR ALL PATIENTS. For (supplied by Mayo Lab) Additional information on Mayo Website: http://www.mayomedicallaboratories.com/test- catalog/Specimen/90172 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

Sotolol SILB 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center

Division of Pre and Post Examination, Page 244 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sphingomyelinase, WBC SMW 6.0 mL DARK Optimal volume 6 mL, minimum 3 mL whole blood. Keep at room Y Children’s Hospital (Niemann Pick A & B) GREEN top temperature. Copy of requisition for Sendout. Collect Monday – Biochemical Genetics Lab 875-2307 Lithium heparin Thursday only or consult Supervisor. tube whole blood SPH Sendout person: Send specimen to Children’s Hospital Lab by 12:00 hrs (specimen must be at Children’s Lab by 12:00 hours Monday – Friday, the day after collection). 1. Send copy of requisition with transport batch. 2. Put colour CW Biochemical Genetics Lab address sticker on TDG container 3. Call BGL at 6048752307 to notify and to expect the package. Spinocerebellar Ataxia Panel MDT 1 Tall LAV Y – for (SCA; SCA1; SCA2; SCA3; ORDERING PHYSICIAN: For predictive/pre-symptomatic Children’s Hospital Inpatients MJD; Machado-Joseph testing, patient must be referred for genetic counseling Molecular Genetics Lab Disease; Machado Joseph (Vancouver ph. 604-875-2157) disease; SCA6; CACNA1A; Optimal: 6 mL whole blood; minimum 2 mL 604-875-2852 SCA7; Ataxin; ATXN; Send at room temperature with Molecular Genetics Lab ATXN1; ATXN2; ATXN3; requisition: ATXN7; http://www.elabhandbook.info/phsa/Files/RequisitionForms%2f1 Olivopontocerebellar _20140214_031358_CWMG_REQ_0000_v4.0_General_Requis Atrophy; OPCA; ition.pdf Cerebelloparenchymal; CPD; CPD1; Menzel; Schut-Haymaker; Schut Haymaker; Holguin) Squamous Cell Carcinoma SCCB 1 RED top Freeze Serum. Send on ice pack. BC Centre For Disease Control Antigen C/O PHSA Laboratories – Lane Level Laboratory SSA (Anti- LA) ANAEB 1 SST Gold top Minimum 1.0 mL serum. Aliquot and Freeze. Send on ice pack. VGH Lab Stability: 3 days @ 4°C.

SSB (Anti – RO) ANAEB 1 SST Gold top Minimum 1.0 mL serum. Aliquot and Freeze. Send on ice pack. VGH Lab Stability: 3 days @ 4°C.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Send CBCD results if available. If results are unavailable at the Stem Cell Culture STMCBB 2 X 10 mL Heparin Pheripheral Terry Fox Lab – Stem Cell Assay time of refer-out, please log in CB4 and enter ;CBC to Terry Fox (GREEN top) blood Or Add Lab 6048776244 604-675-8000 Ext 7746 tubes. More (STAT CBCD Stem Cell Culture For needed if low approval is Terry Fox Lab Will Accept Blood Erythropoietin WBC. required) Until 1500hr Only Room temperature- send within 24-48 hours of collection. Or Must phone to inform terry fox lab that specimen is being N - if ordered Independent Growth sent. Must send on Friday even without approval. If not by Drs.: Erythropoietin approved, then call to cancel. Ezzat, Foltz Copy of requisition for send out. Complete stem cell assay reqs in S. Jackson, forms/reqs section C.Leger, H.Leitch, Ross(Boldt), Ramadan, Hoeshi, H. Merkley & P. Yenson

Bone Marrow No approval

Stercobilinogen Or No longer available. Suggest urine urobilinogen Faecal Urobilinogen

Stool Culture PFAEC Stool in sterile Stool for culture and sensitivity 2 days SPH Microbiology container, no preservative.

Stool Reducing Substances RSUBF Fresh stool Stool should be collected in a container or polyethylene lined Daily SPH Chem diaper. Must be analyzed within 1 hour of collection or freeze for NOT AVAILABLE – MAY 2015 later analysis.

Streptobacillus Serology (Rat STRMON 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Bite Fever Serology)

Strongyloides Serology STRGY 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control

Strontium Not available

Strychnine STRTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 246 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sugar Water Test SWT One Citrate tube See hematology prior to collecting. Must be collected before 1500 Y SPH Order PNHFLO DO NOT SPIN hrs. Copy of requisition to Hem.

Sulfadiazine Unavailable

Sulfatides, Urine NCODE 3 mL random Prior consultation required. Contact CW Biochemical Genetics Y Children’s Hospital – Biochemical Urine on ice Laboratory at (604) 875-2307 for approval. Genetics Lab Room 2F22 Sample must arrive at BCCH before 1200. Do not collect after 0900, Monday – Friday. Sendout person must obtain approval 604-875-2307 STAT and send by cab. Put CW Biochemical Gentics lab address sticker on TDG container. Alert Children’s when sending specimen. 3 ml urine minimum. Collect on ice, freeze and send frozen. Copy of requisition for send out.

Sulfhemoglobin SHGB 1 LAV Discontinued Jan 22/2014. Mon – SPH Special Chem Fri

Division of Pre and Post Examination, Page 247 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sulfocysteine, Urine NCODE 3 mL random Prior consultation required. Contact CW Biochemical Genetics Y Children’s Hospital – Biochemical Urine on ice Laboratory at (604) 875-2307 for approval. Genetics Lab Room 2F22 Sample must arrive at BCCH before 1200. Do not collect after 0900, Monday – Friday. Sendout person must obtain approval 604-875-2307 STAT and send by cab. Put CW Biochemical Gentics lab address sticker on TDG container. Alert Children’s when sending specimen. 3 ml urine minimum. Collect on ice, freeze within 30 minutes and send frozen. Copy of requisition for send out.

Sulfonylureas DRSCB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Acetohexamide Special requests must be indicated in Sunquest Order Entry; copy Chlorpropamide of original requisition. Tolazamide Tolbutamide Glimepiride 20 mL Urine Sunquest Order Entry instructions for Urine samples: Glipizide Glyburide 1. At Container and Specimen Entry: remove “R” and add “UR” Nateglinide Free text request in Result Entry for special orders, e.g.: Repaglinide DRSC1=URINE-;Acetohexamide (example only) or special request (example ONLY): DRSC1=URINE- ;Sulfonylureas and other insulin secretagogues. Attn Dr Schreiber Prov Tox

Sulindic SPQ 1 RED top 2-4 mL serum or urine. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Sulindic SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 248 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sulphamethoxazole SULFB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 249 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Sulpiride SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; SULPIRIDE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Sweat Chloride SWTB Refer patient to Children’s Hospital Children’d Hospital

Synapse 6 mL LAV top Do not spin. Send to Dr. Wilfred Jeffries VGH Lab and

2 RED top Synovial (Joint) Fluid Crystals FCRYSP Glass tube without Avoid Lithium Heparin, EDTA, SST or Oxalate (but fluid will be Mon – SPH Chem anticoagulant (e.g examined regardless of the additive present.) Order includes Fat Fri RED top) Globules and FTYPE. Synovial Fluid Glucose FGL GREY top Only if collected from fasting patient into a GREY top vacutainer 24 Hour SPH Chem (Synovial TP, LD and all other PLUS a concurrent plasma glucose. Chemistry Tests – Not Allowed)

Synthetase Levels Unavailable

Division of Pre and Post Examination, Page 250 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Syphillis Screening (Blood) TPS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - Treponema Pallidum screen C/O PHSA Laboratories – Lane Level Laboratory Treponema Pallidum Babies: minimum 200µL serum (~500 µL Gold SST), venous Confirmatory collection preferred. Syphilis Confirmatory MHATP MHA-TP or Microhaemagg Lutination Treponema Pallidum Test RPR (Rapid Plasma Reagin)

FTA, FTA-Abs TPS and Fluorescent Treponemal Ab TPSC Diagnosis Of Uveitis ART (VDRL) TPPA

Syphillis Screening, CSF VDRLCB 1 mL CSF Refrigerate. Send on ice pack. BC Centre For Disease Control - Treponema Pallidum screen C/O PHSA Laboratories VDRL – Lane Level Laboratory

T3 Renal Uptake - T3ru NCODE 1.0 mL serum Unavailable unless initiated by Biochemist. All patient’s Y Metropolitan Lab demographic information plus attn – dr.mincey must appear on Attn-Dr.Mincey the requisition. Freeze.

T3 (Triiodothyronine), Reverse, NCODE 1 RED top 2 to 4 mL serum preferred minimum 0.4 mL. Freeze serum and Y Specimen Process Center Serum (Reverse T3) send on dry ice. Stability 7 days ambient/refrigerated; 28 days Mayo Medical Laboratories *1 SST Gold top frozen. Ensure Bill 73 is completed and copy of requisition for 3050 Superior Drive NW Mayo Test ID: RT3 acceptable* Sendout bench. Rochester, MN 55901 SPH Sendout: must generate Mayo Clinic Order in MayoLink, 1-800-533-1710 see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Hospitals In-Common Laboratory T3 Total, NCODE 1 SST Gold top 1 mL serum frozen or on ice. Copy of requisition for send out. Inc. Put Fax Result to SPH Lab request sticker on requisition or on (Total T3 57 Gervais Dr. Sunset Printout. Triiodothyronine,Total) North York, ON M3C 1Z2

Phone 416-391-1499 Ext.248 Fax Phone 416-385-1957 T4, Free by Dialysis, Serum NCODE 1 SST Gold top 1 mL serum. Send frozen on dry ice. Ensure Bill 73 is Y Specimen Process Center completed and copy of requisition for Sendout bench. Mayo Medical Laboratories Mayo Test ID: FRT4D SPH Sendout: must generate Mayo Clinic Order in MayoLink, 3050 Superior Drive NW see Supervisor: Rochester, MN 55901 https://orders.mayomedicallaboratories.com/en/login?SAMLart= 1-800-533-1710 NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Additional information on mayo website: http://www.mayomedicallaboratories.com/test- catalog/specimen/91548 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

T7 Do FT4 SPH Special Chem

Taenia Solium Serology CYSTIB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Teichoic Acid Unavailable

Temazepam (Restoril) TMZPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Division of Pre and Post Examination, Page 252 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Terminal-Transferase 3 mL EDTA + 6 Make slides within 30 min. Send spec and slides with BCCA req BCCA (Terminal-Deoxynucleotidyl- Slides to Immun. – BCCA. Transferase)

Tetanus Antitoxin Level TETANB 5 mL SST Gold top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Tetanus Immune Status by Medical C/O PHSA Laboratories – Lane Testing is only available in special circumstances after Discontinued) Microbiologist Level Laboratory consultation with program head 604-707-2622. Lock-Jaw Modify BCCDC’s Specimen Description with Med Micro initials if Approved: E.g. SD0174=BLD-AMMMR

Tetrahydrocannibinol SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Tetrahydrocannibinol SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Thallium-Urine 24 hour NCODE 10 mL from 24 Refrigerate during collection. 10 mL aliquot from well mixed 24 Y Specimen Process Center hour urine, no hour collection. Refrigerate and send on ice pack. Must use clean Mayo Medical Laboratories preservative plastic container and cap with no metal. Stability is 28 days at 3050 Superior Drive NW 4°C. Mayo test ID: TLU Rochester, MN 55901 1-800-533-1710 Ensure Bill 73 is completed and copy of requisition for Sendout bench. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Division of Pre and Post Examination, Page 253 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 0.5 mL lithium heparine plasma. Must check dose time. Theophylline Level THEOP 1 PST VGH Lab Refrigerate plasma. Send on ice pack. (Aminophylline) Sunquest Last Dose format: THEDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE THETLD: ;HH:MM e.g. ;13:50 or UNAVOE Thermoactinomyces vulgaris FTVUL 1 SST Gold top 0.5 mL serum. Refrigerate and send on ice pack to VGH . No Carlsten Lab at Diamond Health need to aliquot if SST. Affix Lung Centre address label to the Care Center via VGH Lab (Faeni Thermophilic vulgaris) package for VGH to forward to Lung Centre. Please provide a 604-875-4111 ext 66455 *1 RED top copy of original requisition. Put Fax Result to SPH Lab request acceptable (must sticker on requisition or on Sunset Printout. be aliquoted)* Do not freeze. Thermophilic-Fungi-Precipitans THFN 1 SST Gold top 0.5 mL serum. Refrigerate and send on ice pack to VGH . No Carlsten Lab at Diamond Health (Farmer’s Lung Antigen) need to aliquot if SST. Affix Lung Centre address label to the Care Center via VGH Lab

package for VGH to forward to Lung Centre. Please provide a 604-875-4111 ext 66455 *1 RED top copy of original requisition. Put Fax Result to SPH Lab request acceptable (must sticker on requisition or on Sunset Printout. be aliquoted)* Do not freeze. Thiazide Screen DRSCB 50 mL urine Refrigerate Provincial Toxicology Center Second screen: DRSC1 =Specimen type-;Specify Drug (Specimen type e.g. URINE-;Thiazide)

Thiethylperazine SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Thiethylperazine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 254 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Thiocyanate TCYAN 1 SST Gold top 1 mL serum. Process STAT. Copy of requisition for send out. Y Provincial Toxicology Center Stat Request (process as per Contact Medical Biochemist on call for STAT approval Ethylenegycol procedure)

Thiopental THIOB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. (pentothal)

820 Sherbrook Street Sendout Tech: check order history to ensure TPMT is not Winnipeg, Manitoba, R3A 1R9 already performed. Send for Approval if history of TPMT (204) 787-1534 report. Must store in fridge, stability is 24 hours @ ambient, and 7 days @ 4°C. Ship between Mon – Wed to ensure safe arrival of the sample. Put Fax Result to SPH Lab request sticker on completed HSC requisition, copy and paste on Intranet browser: \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Requisitions\TPMT HSC Winnipeg.pdf Prometheus Laboratory Thiopurine Methyltransferase NCODE 6mL EDTA whole Test NOT routinely done. Do not send with TPMT enzyme Y 9410 Carroll Park Drive (TPMT Genetics) blood test. Consult Supervisor before sending. San Diego, CA 92121

Tel 1-888 423 5227 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Thioridazine THDZB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) (Mellaril Or Serentil) DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Thioridazine Thiothixene THIOTB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Thrombin Time TT 1 CIT 1.0 mL plasma from full tube collection. Plasma spun within 2 24 Hour SPH Hem hours of collection and tested within 8 hours of collection. If test is not performed within 8 hours, double spin plasma and store at -20°C for up to 2 weeks, ship on ice. Specimen must arrive frozen in polypropylene tube

Thrombophilia DNA (see Factor 5 Leiden)

Division of Pre and Post Examination, Page 256 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Thyroglobulin TG 1 SST Gold top 0.5 mL serum (1.0 mL serum if lipemic) in False Bottom or 13 x Serum: SPH Special Chem 75 Polypropylene tube, freeze serum. Send with ice pack within Mon, (Thyroglobulin Tumor Marker, Battery 3 days or send frozen. Tues, Thyroglobulin by Mass Spec, includes Thurs TG by Mass Spec, TG by MS) thyroglobulin Indicate collection site at Order Entry Modifier field. and Fine TG Battery includes anti- Freeze sample in container if collected outside of Special and thyroglobulin Needle thyroglobulin antibodies Chemistry operating hours. aspirate: anti-thyroglobulin antibodies BIOTINIDASE Mon - Fri Thyroid Binding Globulin TBG 1 mL. Serum No longer available as of April 2002 (0.4-0.5 mL. MINIMUM)

Thyroid Receptor Antibody TSHRA 1 SST Gold top 1 mL serum in False Bottom or 13 x 75 Polypropylene tube, Est. SPH Special Chemistry (Thyroid-Stimulating- freeze serum. Send with ice pack. weekly Antibodies) (workload (Thyroid-Stimulating- dep.) Immunoglobulin –T.S.I.G) (Anti TSH Receptor Ab) (Anti-thyrotropin receptor Ab)

Thyroid Stimulating Hormone TSH 1 SST Gold top or 2 mL serum, send on ice pack. Stable for 7 days at 2-8 °C. 24 Hour SPH Lab (TSH heterophile antibody – TSHB see 1 RED top TSHB is for suspect hypothyroid or hyperthyroid, will reflex order copy of req for Special instruction FT4, FT4 will reflex order FT3 if results and criteria are met. Chem)

Timolol TMLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Tissue Trace Elements CUTIS Tissue Refer to eLab Handbook online for updated instructions or see Children’s Hospital Hepatic Iron (Biopsy) (copper) Children’s Hospital binder on send out bench. Gem Program Iron, tissue Collection vial is the standard Microbiology urine container. 4480 Oak Street FETIS Copper, tissue Send specimens on ice pack for arrival within 2 hours of (Iron) Phone 875-2331 collection and before 3 pm, Monday to Friday or on dry ice if not sent within stated time. See tissue trace elements reqs on Children’s eLab: http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1 _20140203_120221_Tissue%20TE%20Req%20Lower%20Ma inland%20Sep20%2013.doc

Tissue Transglutaminase TTG 1 SST Gold top 1 mL serum. Freeze. Send frozen on ice pack VGH Lab (Anti Endomysial Antibodies) (Anti-Tissue Transglutaminase) (Anti-Gliadin Antibodies) (Celiac Disease Testing) (Gluten Intolerance Test)

Tissue Typing HLAT 1 RED top Room temperature VGH Lab 0.5 mL plasma. Trough ½ Hour Tobramycin Pre Dose TOBPR 1 PST 24 Hour SPH Chem Sunquest Last Dose format: TOBDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE TOBTLD: ;HH:MM e.g. ;13:50 or UNAVOE 0.5 mL plasma. Peak ½ hour post IV infusion – confirm times with Tobramycin Post Dose TPO 1 PST 24 Hour SPH Chem nurse prior to collection Sunquest Infusion format (ITST=Start ITFT=Finish): ITST: ;HH:MM ITFT: ;HH:MM 0.5 mL plasma. Tobramycin Random Level TOR 1 PST 24 Hour SPH Chem Sunquest Last Dose format: TOBDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE TOBTLD: ;HH:MM e.g. ;13:50 or UNAVOE Tocainide NCODE 1 RED top 2 mL serum from RED top. Refrigerate Y VGH Lab 875-4558

Tolbutamide (Orinase) TOLBB 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center

Sunquest Order Entry instructions for Urine samples: 20 mL Random Urine  At Container and Specimen Entry: remove “R” and add “UR”

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Toluene SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Toluene SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Topiramate (Topamax) TOPMB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Indicate if for toxicity. Refrigerate. Send on ice pack.

TORCH Screen (Includes TOXGSB, 2 x 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control Toxoplasma, Rubella, CMV IgG RUBP, top C/O PHSA Laboratories – Lane And IgM and HSV IgG only) CMVSP Level Laboratory ***Also order TOXMSB If HSVIGB Symptoms Indicated*** TOXGSB TORCH STUDIES For 2 x 5 mL SST Gold 2 tubes from Mother. If Mother unavailable, take from baby: 2 mL BC Centre For Disease Control and Newborn top venously or 4 full clotted BD Microtainer tubes (minimum 0.5 mL C/O PHSA Laboratories – Lane TOXMSB Serum accepted) Level Laboratory Toxoplasmosis, Rubella, RUBP Cytomegalovirus, Herpes Virus CMVSP Send Room Temp with Serology Req’s HSVIGB

Total T4 FT4 1 SST Gold top or 1 mL serum, store and send refrigerated. Stable for 7 days at 2-8 24 Hour SPH Chemistry Replaced by free T4 or Free °C. 1 RED top Thyroxine

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Total Testosterone TES 1 RED top 2.0 mL serum. Minimum 0.5 mL. Adult males should be Mon – Fri SPH Special Chemistry (Testosterone) collected before 10:00 AM but not mandatory. If Bioavailable testosterone only, do not log in until clarification from ordering *no gel tubes* physician. Send on ice pack within 5 days of collection or send frozen.

Toxicology Testing DRSCB 1 RED top Refrigerate and send on ice pack. Provincial Toxicology (Toxicology Drug Screen, Sunquest LIS Order Entry: If urine sample: Remove RED I or Toxicology screen) default tube at Route screen and add UR container type. Random urine Second screen: DRSC1 =Specimen type-;Specify Drug (Specimen type e.g. BL=blood, URINE=urine, PLS=plasma)

Toxocara Serology Or Visceral VLVM 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Larva Migrans C/O PHSA Laboratories – Lane Level Laboratory

Toxoplasmosis – IgG Serology TOXGSB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control - Toxoplamsa Gondii IgG C/O PHSA Laboratories – Lane Level Laboratory

Toxoplasmosis –IgM Serology TOXMSB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Toxoplamsa Gondii IgM C/O PHSA Laboratories – Lane

Level Laboratory

Toxoplasma Gondii PCR TOXOP 1 mL CSF and Give specimen to SPH Microbiology along with copy of Y BC Centre For Disease Control requisition for ordering and Medical Microbiologist approval By Med. Zoonotic Diseases & Emerging 5 mL SST Gold top (Microbiology TRAP test). Micro. Pathogens for TOXMSB Modify BCCDC’s Specimen Description with Med Micro initials if TRAP Approved: E.g. SD0116=BLD-AMMMR (search for MM code)

TPNM2 (TPN Monday, Total TPNM2 1 PST Light green Tests include: URE, CRE, NA, K, CL, TCO2, GLUC, ALB, PALB, 24 Hour SPH Chem parenteral nutrition – Monday) top and TBIL, ALKP, CA, PO4, ALT, TRIG, MG, 1 SST Gold top

TPNT2 (TPN Thursday) TPNT2 1 PST Tests include: URE, CRE, NA, K, CL, TCO2, GLUC, PALB, ALKP, 24 Hour SPH Chem PO4, MG,

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Tramadol SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; TRAMADOL SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Transferrin Isoelectric Focusing TIF 1 RED top Centrifuge and separate minimum 200 µL serum and freeze. Ship Children’s Hospital serum frozen. Copy of requisition for Send out. (Carbohydrate Deficient Specimen Receiving 2J20. Glycoprotein Syndrome, CDGS, Carbohydrate deficient transferrin, Congenital Disorder of Glycosylation, Transferrin Isoforms)

Transferrin isooforms, Ref. Out TIFO 1 SST Gold top or 1 mL serum. Freeze and send on ice pack. Y Children’s Hospital Ref out to Mayo Apo CIII , Apolipoprotein CIII RED top

Tranylcypromine (Parnate) TRNLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Tranylcypromine Trazodone (Desyrel) TRZDB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage & time of last dose. Specimen should be collected prior to (Tetracyclic Antidepressant) next dose. Refrigerate. Send on ice pack. If patient of Dr. Misri, already pre-approved. DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Trazodone Treponema Pallidum TPDE Specify sample Refrigerate. Send on ice pack. BC Centre For Disease Control Microscopy type at RESOE. Treponema Pallidum NAT TPNAT 6 mL EDTA Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control (Syphillis PCR) (or CSF, FLUID, Modify BCCDC’s Specimen Description with Med Micro initials if by Medical SST, LAV, MISC; Approved: E.g. SD0080=BLD-AMMMR Microbiologist specify specimen

type) Triazolam (Halcion) TRZLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

DRSCB >2mL Urine Triazolam

Trichinosis Serology TRICHB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Trichinella Serology) Level Laboratory

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Trichloroethanol TRCHLB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center (Chloral Metabolites) requisition for send out.

Must Specify Which Drug. CLMPRB Serum must be separated within 2 hours of collection. Stable in fridge. Send on Sunquest transport batch. Use Separate Test Code DESIPB AMIB= Amitriptyline IMPB CLMPRB= Clomipramine NORTIB DESIPB= Desipramine DXPNB=Doxepine TRMPB IMPB= Imipramine NORTIB= Nortriptyline TRMPB=Trimipramine

Tricyclic Antidepressant TCADS 1-2 mL serum. Order from EMER or ICU, do STAT TCAS. Revised Jul/02 SPH Screening. Use This When RED top tube TEST NOT AVAILABLE AS OF APR 2015. Specific Antidepressant Not Known.

Tricyclic Screen, Urine LCTCA Random urine Refrigerate urine. Y Provincial Toxicology Center and Sunquest order entry: removed “R” default container and add DRSCB “UR” container.

Trifluoperazine (Stelazine) TRFLB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage & time of last dose. Specimen should be collected prior to next dose. Refrigerate. Send on ice pack.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Triglycerides TRIGF 1 SST Gold top 0.5 mL serum. 24 Hour SPH Chem For fasting status enter PF if patient has been fasting 10-14 hours. If the patient has been fasting for less than 10 hours (or not at all) enter ;Fasting for X hours. For example, if the patient came in for bloodwork at 1000 and they had eaten at 0800 you would enter ;Fasting for 2 hours”. If the fasting status is unknown because the sample has been dropped off or sent in and the patient is unavailable to ask when they last ate enter UNAVOE.

Triglycerides, fluid FTRIG 1 PST Light green 0.5 mL fluid. 24 Hour SPH Chem top, SST Gold top, FTYPE: PLEUR; DIA; PERIT; BAL; PCF RED top or non Synovial fluid TRIG not available. preservative container

Trimeprazine SPQ 1 RED top 2-4 mL serum or urine. Refrigerate. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER or ;Urine SPQ1=;Trimeprazine SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

DRSCB >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Trimeprazine 1 RED top Trimethoprim TRIMEB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out. >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Trimethoprim Trimethylamines TRMA Random urine Call Children’s Hospital for handling instructions. Y Children’s Hospital Fish Odour Disease 1 RED top Trimipramine & TRMPB 2-4 mL serum or urine. Must specify which drug. Y Provincial Toxicology Center Desmethyltrimipramine Ward should record information on dosage & time of last dose. (Surmontel) (Tricyclic Specimen should be collected prior to next dose. Refrigerate and Antidepressant) send on ice pack. >2mL urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Trimipramine 1 RED top Tripelennamine TRPLB 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) (Pyribenzamine) >2mL Urine DRSCB Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Tripelennamine Tropheryma Whippelii Serology TRWH TEST NOT AVAILABLE @BCCDC ZEP as of APR 2013. (Whipples Disease Serology)

Troponin T CTROPT 1 PST Light Green 1.0 mL lithium heparin plasma. Stable for 24 hours at 2-8 °C. 24 Hour SPH Chem Lithium Heparin

Trypanosoma Cruzi Serology TRYPB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (Chagas Disease Serology)

Trypanosoma Serology (So TRYPAN 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control African Sleeping Sickness)

Trypsin, Immuno-Reactive IMMTRB (see Immunoreactive Trypsin)

Trypsinogen (see Immuno Reactive Trypsin) Children’s Hospital Tryptase (Total) TRYPC 1 RED top 2 mL serum (minimum volue is 0.5 mL). Refridgerate and send 2 weeks Y (Mast Cell Tryptase) on ice pack. Plasma not suitable. Anaphylaxis collections must be collected 15 minutes to 3 hours post allergic reaction. Separate serum ASAP.

Undecalcified Bone Biopsy (see Hematology)

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Unsaturated Transcobalamins TRCBB 1 RED top 1 mL serum from RED top. Approval required. Minimum 0.2 mL Y Quest Diagnostics Chantilly (Transcobalamin I,II) serum. Freeze. Copy of requisition for send out. Send on ice Nichols Institute pack. Refrigerated stability is 4 days; Frozen is 30 days. 14225 Newbrook Dr. Chantilly, VA 20151-2228 More information from Quest Diagnostics: U.S.A http://www.questdiagnostics.com/testcenter/TestDetail.action?ntc= 928 Mandatory MSP pre-approval required for Outpatients 1(800) 336-3718, Client Services (request to be done by patient Dr.). For Outpatients, do not ext. 3000 collect without MSP preapproval if test is ordered alone. Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker

Unstable Hemoglobin ISOPB 2 EDTA Must be preapproved and booked. Must send within same day Y Complex Hematology (Isopropanol Stability Test) collection. Available Monday to Wednesday. –updated Nov23/04. Children’s Hospital Sunquest Order Entry last screen: ISOPB1: Y or N (preapproved) ISOPB2: Path: CDRJF, CDRNA, CDRSV, CDRPV (by which Dr.)

Urea, 24 hour urine URU 24 hour urine, no Refrigerate during collection. 3 mL aliquot from well mixed 24 Mon – Fri SPH Chem preservative hour collection (do not send whole collection), centrifuge and Add CRU, (Unsuitable if aliquot supernatant to 12 x75 plastic tube. Referring sites need to UTIM collected in Acid) include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack.

Urea, random urine URR Random urine 3 mL random urine, centrifuge and aliquot supernatant to 12 x75 24 Hour SPH Chem plastic tube. Refrigerate

Uric Acid URCA 1 PST 0.5 mL plasma 24 Hour SPH Chem

Uric Acid, fluid FURCA PST, SST, RED or 0.5 mL fluid. 24 Hour SPH Chem (fluid uric acid) non preservative FTYPE: PLEUR; DIA; PERIT; BAL; PCF container Synovial fluid uric acid not available; see Fluid Crystal if order is for Uric Acid crystals (monosodium urate) in Synovial Fluid.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Refrigerate during collection. Measure 24 hour volume. Aliquot Uric Acid, 24 hour urine UAU 24 hour urine, no Mon – Fri SPH Chem 3mL for urine creatinine, centrifuge and aliquot supernatant to 12 preservative Add CRU, x75 plastic tube. Aliquot approximately 100 mL from well mixed UTIM 24 hour collection and adjust the pH of aliquot to 9-10 using 10 mol/L NaOH (do not send whole collection) for heating at SPH.

Referring sites need to include 24 hour volume, patient’s height and weight on requisition. Refrigerate and send on ice pack. Uric Acid, random urine UARU Random urine Order code includes urine creatinine and ratio. 3 mL random 24 Hour SPH Chem urine, centrifuge and aliquot supernatant to 12 x75 plastic tube. Refrigerate

Urinalysis (Urine routine and RMU Random urine 10 mL random urine in urine tube. Must be process within 4 24 Hour SPH Chem. microscopic, Urine R&M, Urine hours. Minimum 1 mL urine. RM)

Urine Culture PPUR Sterile urine Refrigerate 1-2 days SPH Microbiology container

Urine, GC/Chlamydia PCTNGU Sterile urine Refrigerate Mon – Fri SPH Microbiology container 24 hr to result.

Urine culture if LEU or NIT + URNEG + Sterile urine + Refrigerate 24 Hour SPH Chem and Micro RMU urinalysis tube

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Urine Cytology CYTUR Sterile urine Put specimen inside biohazard bag and a copy of original SPH Cytology (Cytology urine) container requisition for Cytology in specimen bag pouch. Refrigerate in Cytology bin. SPH Outpatient process: 1. Use Order Code CYTUR, order separately from other orders (different Accession number). 2. Pre-label the Cytology container with the specimen barcode (including the ones the patient takes away if unable to void immediately). 3. Put the Master label and PHN label on the requisition. 4. Date and Time of Collection: If Patient will provide If Patient is not able to void, specimen in OP, then then A. Write collection Date A. Provide a copy of the and Time on requisition, requisition. B. Highlight the Date and B. Provide copy of Time of collection, requisition in specimen C. Instruct the patient to pouch. drop off the specimen at C. Instruct the patient to the front counter with drop off in OP Urine Requisition. drop off tray. 5. Repeat steps 1-4 if order is Cytology X3. So a total of 3 labelled containers in separate biohazard bags with a copy of the requisition in each specimen pouch. 6. Remind patient to tighten the lid properly, and ensure it is not leaking to avoid recollection. Bring urine back to our lab after collection.

Urine Reducing Substances REDCR 12 mL fresh Collect fresh random urine in Fisher brand Urisystem. Sample 24 Hour SPH Chem NOT AVAILABLE – MAY/15 RMU random urine should be analyzed immediately.

Urobilinogen URB 10 mL urine No longer available as of 12/2017 24 Hour SPH Chem

Valium (Quantitative) DZPNB 4.0 mL serum Assayed weekly. Y Provincial Toxicology Center Lab [Diazepam] (Quantitative) or 20 mL urine Ward should record information on dosage & time of last dose. (Qualitative) Specimen should be collected prior to next dose. Refrigerate.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) 0.5 mL lithium heparin plasma. Refrigerate, dose time required. Valproic Acid Level (Total) VAL 1 PST 24 Hour SPH Chem Sunquest Last Dose format: (Depakene, Valprovate, VALDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE Depakene, Dival Proex VALTLD: ;HH:MM e.g. ;13:50 or UNAVOE Sodium, Epival)

Valproic Acid (Free) NCODE 1.0 mL Lithium Refrigerate, dose time required. Do not log in valproic acid total. VGH Lab Heparin plasma Trough ½ hour Vancomycin Pre Dose VANPR 1 PST 24 Hour SPH Chem Sunquest Last Dose format: VANDLD: ;DD Mmm YYYY e.g. ;31 Mar 2014 or UNAVOE VANTLD: ;HH:MM e.g. ;13:50 or UNAVOE Peak 3 hour post IV infusion – confirm times with nurse prior to Vancomycin Post Dose VPO 1 PST 24 Hour SPH Chem collection. Sunquest Infusion format (ITSV=Start ITFV=Finish): ITSV: ;HH:MM ITFV: ;HH:MM Varicella Zoster Virus –IgG VZIGB 5 mL SST Gold top Refrigerate. BCCDC (Chickenpox IgG, VZV)

Varicella Zoster Virus – PCR PVPCR 1 mL CSF or skin Freeze CSF. Forward to Virology. Mon – Fri SPH Virology Lab (non blood) swab

Vascular Endothelial Growth NCODE 1 EDTA 0.3 mL EDTA plasma minimum, separate and send frozen. MSP Y Quest Diagnostics Nichols Institute Factor (VEGF) preapproval by patient Dr required. Stability: 4 hours RT, 48 33608 Ortega Highway hours refrigerated, 1 year Frozen. San Juan Capistrano, CA 92690- http://jdos.nicholsinstitute.com/dos/SanJuanCapistrano/test/9002 6130 9. 1(800) 553-5445 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone. Complete Quest Diagnostics’ Test Request Form and Put Fax Result to SPH Lab request sticker

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) Translational Trials Development Quantitation of Vascular NCODE 5 mL SST Gold Collect before 1100 Monday –Friday. Spin and aliquot serum. Y and Support Laboratory (TTDSL) Endothelial Growth Factor D top Send at RT overnight. Sample must arrive the next day. Cincinnati Children’s Hospital (VEGF-D) **If sample can not be sent on the same day of collection, freeze Medical Center serum and send the next day on dry ice** 240 Albert Sabin Way, S11.400, MLC 7013 Specifically for Notify performing lab prior to sending sample. (513) 636-5998. Cincinnati, OH 45229 lymphangioleiomyomatosis Complete Cincinnati Children’s Hospital Requistion form and Put Fax Result to SPH Lab request sticker Cost is $376.75 USD. https://research.cchmc.org/translationalcores/sites/bmidrupalptra nslationalcores.chmcres.cchmc.org/files/VEGF- D%20Form_Updated%20July%202015.docx

Vasoactive Intestinal NCODE 6 mL EDTA Optimal 2 mL EDTA plasma from Fasting Patient. Send frozen Y Specimen Process Center Polypeptide – VIP on dry ice. Approval required for all patients. Ensure Bill 73 is Mayo Medical Laboratories completed and copy of requisition for Sendout bench. 3050 Superior Drive NW Mayo Test ID: VIP SPH Sendout: must generate Mayo Clinic Order in MayoLink, Rochester, MN 55901 see Supervisor: 1-800-533-1710 https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A Additional information on Mayo website: http://www.mayomedicallaboratories.com/test- catalog/specimen/8150 Mandatory MSP pre-approval required for Outpatients (request to be done by patient Dr.). For Outpatients, do not collect without MSP preapproval if test is ordered alone.

VDRL Test –CSF ( Syphilis- VDRLCB CSF Refrigerate. Send on ice pack. BC Centre For Disease Control CSF Only, Serology, RPR – C/O PHSA Laboratories – Lane CSF Only) Level Laboratory

Venlafaxine VENLAB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Vigabatrin (Sabril) VIGB 1 RED top 2-4 mL serum or urine. Ward should record information on Y Provincial Toxicology Center dosage, time of last dose, and other meds. For therapeutic drug monitoring specimens must be drawn at trough. Serum must be separated from cells within 2 hours of drawing. Refrigerate. Send on ice pack.

DRSC >2mL Urine Refrigerate if not done immediately. Send urine on ice pack. Sunquest LIS Order Entry: *If collection time is now (N), enter time as one minute before in order to get to the route screen* Remove RED (R) default tube at Route screen and add UR container type. Second screen: DRSC1 = URINE-;Vigabatrin

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Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Viral Hemorrhagic Fever NCODE1 2x 6 mL EDTA Refer to “Phlebotomy and Sample Transport for Suspect Viral Y – for protocol ;EDTA 1x PST light green Hemorrhagic Fever (Including Ebola Virus Disease) Patients” collection by

Ebola 1x CIT light blue procedure. All unpacking and repacking of the secondary Med. Micro VHF protocol, Ebola PCR, PCR pressure vessel must be done in the Class II Biological Safety or Medical Ebola protocol Cabinet in Microbiology TB room. Leader. NCODE2 2 x 5 mL SST Gold ;EDTA top (label with non 2x 3 mL EDTA for Ebola PCR to BCCDC must be repackaged in 2x 6 mL EDTA to BCCDC Ebola barcode labels) another Category A TDG compliant system (e.g. STP-100 or PCR STP-130 Category A Shipping System ) by a TDG Certified 2x 5 mL Gold SST to BCCDC Employee (from Microbiology or Core Lab) according to TDG BCCDC will forward to Winnipeg NCODE3 4x blood culture ;GOLD bottles (2 sets) for requirements. SPH Med Micro will contact BCCDC Med SST for Micro Micro to arrange for specimen receipt at BCCDC.

Ebola 1x 3 mL PST for VGH must be packaged in a Category A TDG serology compliant system (e.g. STP-100 or STP-130 Category A Shipping System) by a TDG Certified Employee (from Microbiology or Core Lab) according to TDG requirements. Make plus other orders: sure the bright sticker “Suspect VHF” is on the secondary polypropylene pressure vessel lid or Disposable 2-Part CBCD Secondary Pressure Vessel (if using STP-130 system). Scan MALS the specimen (or the extra label) on a Transport Batch to VH. VHFRMP Phone VGH 604-601-4407 to inform them to expect a suspect COAGB VHF sample. PBLODx2 MSJ: Package the polypropylene Secondary Pressure Vessel SPH Lab Accessioning (TDG container) with STP-100 packing system to SPH. Make sure the bright sticker “Suspect VHF” is on the secondary polypropylene pressure vessel lid. Ensure proper TDG Category A labelling and documentation. Notify SPH Lab to expect a suspect VHF sample.

Viral Load (HIV) VLB 6 mL EDTA Lav 4 mL EDTA plasma, minimum 2.0 mL. After hours and referring Mon – Fri SPH Virology Lab Labs, aseptically separate within 4 hours of collection into sterile Result in plastic cryovial. 5 days Freeze at –20°C, send frozen.

Visceral-Larva-Migrans Of VLVM 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Antibody Against Toxocara (Toxocara Serology)

Division of Pre and Post Examination, Page 273 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Viscosity VISPB 3 RED top at 37°C Minimum 3.0 mL RED top Serum. Collect at 37°C. Deliver Mon – Fri SPH Special Hem immediately to Bone Marrow Hematology lab. Specimens may Immunology remain in 37°C water bath until next morning. Referring sites: Keep at 37°C for 1 – 3 hours (may be longer). Allow specimen to clot completely (Rim clot if necessary). Remove serum (without cells). Indicate specimen process @37°C on label. Refrigerate if sent overnight.

Vitamin A VITAB 1 RED top 2 mL serum, minimum 300 µL from RED top. Approval required Y Children’s Hospital (Retinol Or Retinoic Acid) wrapped in foil for all patients. Copy of requisition for approval. N if from

Protect barcoded specimen from light with aluminium foil, PCF (Cystic put another label on outside (on aluminium foil). Freeze and Fibrosis *1 DRK GRN send frozen. Clinic) Lithium or Na Hep acceptable* Fasting preferred Hospitals In-Common Laboratory Vitamin B1 VITB1B Tall EDTA LAV 2 mL EDTA plasma preferred minimum 1 mL plasma. Protect 10 days Y Inc. from light – wrap vial in foil. Store plasma frozen and send frozen (RBC Transketolase – 57 Gervais Dr. on dry ice. Stability 5 days refrigerated, 6 months frozen. Thiamine Pyrophosphate North York, ON M3C 1Z2 Stimulation) Additional information on HICL website: http://www.HICL.on.ca/search_tcna.asp?tcstring=vitb1 Phone 416-391-1499 Ext.248 (Thiamine) Fax Phone 416-385-1957 Copy of requisition for send out. Put Fax Result to SPH Lab

request sticker on requisition or on Sunset Printout.

Thiamin (Vitamin B1), Whole Blood requests: Specimen Process Center Mayo Test ID: TDP 1. Fasting-overnight (12-14 hours) (infants-draw prior to next Mayo Medical Laboratories feeding). Water can be taken as needed. 3050 Superior Drive NW 2. Invert 8 to 10 times to mix blood. Rochester, MN 55901 3. Transfer whole blood into plastic vial (if EDTA vial is glass) 1-800-533-1710

Freeze Whole Blood EDTA – stability 14 days frozen, ambient or HICL will forward specimens to refrigerated. Ensure Bill 73 is completed and copy of Mayo, send direct if stability/delay is requisition for Sendout bench. a concern. SPH Sendout: must generate Mayo Clinic Order in MayoLink, see Supervisor: https://orders.mayomedicallaboratories.com/en/login?SAMLart= NDB4bVdXsqHYZ01o60GsV63Il9HvdUI5NzA2QjA0QkQ2MTlG QTgyREYy%0A

Division of Pre and Post Examination, Page 274 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Vitamin B2 (Riboflavin) (Red Not available as of Oct01/05 Cell Glutathione Reductase) Hospitals In-Common Laboratory Vitamin B6 NCODE 1 Tall EDTA LAV 2 mL EDTA plasma. Protect from light – wrap vial in foil. Store 10 days Y Inc. (Pyridoxal Phosphate) and send plasma frozen on dry ice. (VITB6 57 Gervais Dr. (Pyridoxine) blocked Additional information on HICL website: North York, ON M3C 1Z2 for SPH) http://www.HICL.on.ca/search_tcna.asp?tcstring=vitb6 Phone 416-391-1499 Ext.248 Copy of requisition for send out. Put Fax Result to SPH Lab Fax Phone 416-385-1957 request sticker on requisition or on Sunset Printout.

Vitamin B12 Total See 1 SST Gold top 1.0 mL serum. Send on ice pack. Copy of requisition for Y VGH (Total B12, Supervisor approval. B12 can only be ordered after Medical Biochemist Cyanocobalamine, Approval in Sunquest GenLab ORM. Cobalamin)

Vitamin B12, Active B12ACT 1 SST Gold top 2– 4 mL serum. Send on ice pack. 3 times SPH Virology (Holotranscobalamin, Active per week. Stability: 16 hours at room temperature, 3 days @ 2-8°C, longer B12, HoloTC, Holo TC, if stored frozen at -20°C. Active B12, Active-B12)

Hospitals In-Common Laboratory Vitamin C (Ascorbic Acid) NCODE 2 mL Sodium Patient must not consume any vitamin supplements for 24 hours 10 days Y Inc. Heparin (DARK before the specimen is drawn. Collect and centrifuge at 4c. 57 Gervais Dr. GREEN) plasma. Separate and freeze ASAP. Protect from light – wrap in foil. North York, ON M3C 1Z2 Store and send frozen on dry ice.

Additional information on HICL website: Phone 416-391-1499 Ext.248 http://www.HICL.on.ca/search_tcna.asp?tcstring=vitc Fax Phone 416-385-1957 Copy of requisition for send out. Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Division of Pre and Post Examination, Page 275 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Vitamin D VITD3 1 SST Gold top 0.5 mL serum. Freeze. Send with ice packs. SPH Lab 25 Hydroxy-Cholecalciferol Children < 1 year old – send to Children’s Hospital. (Forward (Vitamin D3) referred-in specimens without Login). (VIT D or 25-Hydroxy-Vit-D) (Vitamin D 25-OH) Order VITD3R only if Sunquest would not allow VITD3 ordering.

VTD3SP Vitamin D3 Self Pay: 1. Login the test code VTD3SP 2. Generate invoice for patient to take to cashier to pay. 3. Patient comes back with the payment receipt, make a copy of the payment receipt and then collect the sample. 4. Receipt will then be attached to the billing edits (extract) to finance to reconcile receipt with the payment. (If OP Clerk forgot to generate invoice and patient did not prepay then Billing Clerk needs to make a notation on the billing edits extract to invoice the patient.)

Vitamin E VITEB 0.5 mL plasma A copy of the original requisition must be sent to professional staff Y Children’s Hospital (LITHIUM Heparin) for approval. (Alpha Tocopherol) N if from is preferred but Keep tube completely covered with foil while clotting and PCF (Cystic serum is centrifuging. Transfer plasma or serum to aliquot tube also Fibrosis acceptable. wrapped in foil, put another specimen label on the outside, on the Clinic) - protect from aluminium foil. Freeze plasma or serum immediately. Send light with frozen and completely wrapped in foil. aluminium foil

Vitamin-K Not available

VMA (Vanillylmandelic Acid, 24 VMAU 50 mL aliquot of a Collect in collection bottle containing 15mL of 6N HCL. If not Y – if more VGH Lab hour) 24 HR urine collected in acid, acidify in lab, to pH 2 – 4, only if entire collection than one of 3 Add CRU, collection in 15 mL is received. Acidification must be performed within 12 hours tests UTIM of 6 mol/L HCL after completion of 24 hr urine collection. Aliquot 50 mL of a well- ordered. CAT mixed 24hr collection, refrigerate, send on ice pack. or VMA or UMETA. If VMA, Random urine VMAR 50 mL aliquot of Acidify in lab to pH 2 – 4. Acidification must be performed only one of 3, random urine within 36 hours of random urine collection. Minimum 12 mL, no approval refrigerate, send on ice pack. needed

Division of Pre and Post Examination, Page 276 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est) UBC Lab via VGH Lab Voltage Gated Calcium NCODE 5 mL SSTtube 2-5 mL serum. Send frozen on ice pack. Y 604-822-7175 Channel antibodies Ordering physician should have filled out requisition http://med-fom- neuroimmunology.sites.olt.ubc.ca/files/2016/02/NeuroimmLab_R eq_v5.0-20170228-1.pdf Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Voltage Gated Potassium VGKCB 1 RED top or 1 mL serum minimum. Prior approval is required. Freeze serum, Y Dr. Fritzler Channel (VGKC) send frozen on dry ice. Mitogen Advanced Diagnostics 1 SST Gold top Laboratory Use Mitogen Diagnostics Laboratory Requisition (in the HMRB 431 – 3330 Hospital Dr. NW Requisitions Folder of the Accessioning Folder). Calgary, AB, T2N 4N1 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout. Phone 403-220-4582

Von Willebrand Multimer UNAVAILABLE See hematology prior to collecting. Copy of requisition to Hem. Y VGH Lab Analysis Hematology

Von Willebrand Disease NCODE 6 mL EDTA or 7 mL Only orderable via Hemophilia clinic. Request must come from Attention: Jayne Leggo Genotype Testing ACD B one of Hematologists Drs. Ezzat, Jackson, Ross(Boldt), Leger, Department Of Pathology, Leitch, P. Yenson , Hoeshi or H. Merkley. Must provide Richardson Laboratory, Room 201 Hemophilia A and B genotype testing requisition (see forms/reqs) Queen’s University, Kingston, must send on same day collection. If sample is collected on Ontario, K7L 3N6 Friday, freeze whole sample and send frozen on Monday. Tel: 613-533-3187 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

VWF Propeptide NCODE 6 mL CITRATED Only orderable from Hemophilia clinic. Request must come from Y Attention: Colleen Notley plasma one of the Hematologists Drs. Ezzat, Jackson, Ross (Boldt), Department Of Pathology, Leger, Leitch, P. Yenson, Hoeshi and H. Merkley. Forward Richardson Laboratory, Room 201 specimen to SPH Special Coag during dayshift or freeze plasma Queen’s University, Kingston, on shift or weekends. Ontario, K7L 3N6 If not performed at SPH, send frozen on dry ice to Ontario. Tel: 613-533-2822 Put Fax Result to SPH Lab request sticker on requisition or on Sunset Printout.

Division of Pre and Post Examination, Page 277 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Voriconazole VORIB 1 mL EDTA Plasma Collect trough levels 12 hours after the last dose and prior to the Weekly – SPH Special Chem Lab next dose. Centrifuge ASAP. Freeze plasma. Date and time of Wed. last dose and dosage of voriconazole preferred but not mandatory.

Warfarin (Coumadin) WRFNB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

Weil-Felix-Test RICKRB 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control (see Rickettsial Infection) C/O PHSA Laboratories – Lane Level Laboratory

West Nile Virus Serology WNGS 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control and C/O PHSA Laboratories – Lane (WNV Antibody) **Do not spin or open tube** WNMS Level Laboratory **Order only between June 01 – October 30**

West Nile Virus – PCR MISPCR 6 mL EDTA tube Do not spin or aliquot. Send whole specimen on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane (Virology PCR Amplification, Sunquest Order Entry: Level Laboratory WNV PCR, WNV NAT) SPEC5=PLS **Order only between June 01 – SPVIR2=;West Nile virus, transplant (only include TRANSPLANT October 30** comment if Pre-Renal) Example Only Western Equine Encephalitis WEE 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control Serology C/O PHSA Laboratories – Lane

Level Laboratory

Division of Pre and Post Examination, Page 278 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Whooping-Cough-Serology PERSSB 5 mL SST Gold Refrigerate. Send on ice pack. BC Centre For Disease Control (Pertussis) Top tube C/O PHSA Laboratories – Lane ***Not Available At BCCDC*** Level Laboratory

Widal Not available

Whipple’s Disease By PCR NCODE One EDTA tube Complete requisition includes patient diagnosis and/or clinical MICROBIOLOGY Will Refer Out (Tropheryma whipplei (optimal specimen history. Give to microbiology. to Winnipeg via BCCDC. Polymerase Chain Reaction) is a tissue biopsy)

ZAP-70 NCODE 2X7ML EDTA OR Copy of requisition to immunology. Store at room temp. If Y VGH – Flow Cytometry Lab 2 NA Heparin collection is on Friday, consult immunology tech. ***TEST NOT AVAILABLE*** GREEN top,or 2 ACD A.

Yellow Fever Serology YF 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Yersinia Serology YERSB 5 mL SST Gold top Refrigerate. Send on ice pack. Y –TRAP BC Centre For Disease Control Modify BCCDC’s Specimen Description with Med Micro initials if by Medical C/O PHSA Laboratories – Lane Approved: E.g. SD0113=BLD-AMMMR Microbiologist Level Laboratory

Yersinia Pestis Serology PLAGUE 5 mL SST Gold top Refrigerate. Send on ice pack. BC Centre For Disease Control C/O PHSA Laboratories – Lane Level Laboratory

Zika Virus Serology ZIKAB 6 mL EDTA Request manual requisition indicating: both the travel and clinical Y BC Centre For Disease Control 5 mL SST Gold top history, including the date of onset of symptoms. The onset date is C/O PHSA Laboratories – Lane extremely important to ensure appropriate testing. See Instructions. Level Laboratory Zika Virus NAT ZMZIKA Sample collection instructions: 1. During acute symptomatic infection order both ZIKAB and ZMZIKA and collect: a) 5ml EDTA purple top blood tube b) 5 ml gold top serum separator tube 2. If symptoms have resolved order ZIKAB and collect: a) 5 ml gold top serum separator tube only

Division of Pre and Post Examination, Page 279 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Zinc, Urine ZNU 24 HR collection in Aliquot into acid cleaned 250 mL plastic bottles. All equipment Y Children’s Hospital 15 mL OF 6 mol/L used in collection should be acid washed. Affix urine creatinine Add CRU, HCL result label with CRR result before sending out. UTIM in acid cleaned Copy of requisition for approval. bottle Copy and paste path to Intranet browser: \\vch\departments\Chemistry (Dept PHCLAB)\Accessioning\Miscellaneous Collection Procedures\Childrens_Hospital_24 HR URINE Collection Protocol March 9_09.pdf

Zinc, Serum ZN 1 NAVY BLUE Centrifuge x2 Y Childrens Hospital metal free 1st pour into navy vacutainer tube nd vacutainer tube 2 pour into falcon polypropylene tubes Specimen Receiving 2J20 1.0mL minimum Serum Store at 4 °C and transport on ice. If not sending within the week, freeze and transport frozen on dry ice. See Children’s trace elements collection: http://www.elabhandbook.info/phsa/Files/AdditionalFiles%2f1_201 40207_113746_Blood%20Collection%20for%20Trace%20Elemen ts%20rev%20Dec%202013.doc Should be drawn at least 6 to 8 hrs after zinc tablet is given. No gross hemolysis Hospitals In-Common Laboratory Zinc Protoporphyrin NCODE 1 NAVY BLUE Centrifuge x2 10 days Y st Inc. K2EDTA 1 pour into navy vacutainer tube 57 Gervais Dr. metal free nd 2 pour into falcon North York, ON M3C 1Z2 vacutainer tube Phone 416-391-1499 Ext.248 Copy of requisition for send out. Put Fax Result to SPH Lab Fax 416-385-1957 request sticker on requisition or on Sunset Printout. Ziprasidone SPQ 1 RED top 2-4 mL RED top serum or urine. Refrigerate and send on ice pack. Y Provincial Toxicology Centre Sunquest Lab LIS Order Entry: SD0177=SER or ;Urine SPQ1=; ZIPRASIDONE SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Division of Pre and Post Examination, Page 280 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Test Sunquest Specimen Instructions TAT/ Freq Approval Destination Code (Est)

Zomepirac SPQ 1 RED top 2-4 mL RED top serum. Refrigerate Serum. Send on ice pack. Y Provincial Toxicology Center Sunquest Lab LIS Order Entry second screen: SD0177=SER SPQ1=;Zomepirac SQDLD=;DD Mmm YYYY e.g. 31 Mar 2015 or UNAVOE SQTLD=;HH:MM e.g. 13:50 or UNAVOE

Zopiclone (Imovane, Lunesta) ZOPB 1 RED top 2-4 mL serum or urine. Refrigerate and send on ice pack. Copy of Y Provincial Toxicology Center requisition for send out.

PHC Test Directory Revision date: May 24, 2018

Division of Pre and Post Examination, Page 282 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Other Health Authority and Test Reference Links:

VCH: http://sunset/labrefdocs/test_information.pdf

BC Children’s Hospital eLab Handbook: http://www.elabhandbook.info/phsa/Default.aspx

BCCDC Guide to Programs and Services: http://www.elabhandbook.info/phsa

Mayo Medical Lab Test Catalog: http://www.mayomedicallaboratories.com/test-catalog/index.html

Quest Diagnostics: http://jdos.nicholsinstitute.com/dos/SanJuanCapistrano/

Mitogen Advanced Diagnostics: http://www.mitogen.ca/diagnostic.html

Hospital In Commons Laboratory: http://www.hicl.on.ca/resources.shtml

Calgary Laboratory Services (CLS) http://www.calgarylabservices.com/lab-services-guide/lab-tests/

Division of Pre and Post Examination, Page 283 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Division of Pre and Post Examination, Page 284 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Revision/Review History: Date Revision Type By Summary of Changes (Refer to LabQMS for archives) Sep 18 2017 Minor Jennifer Clarke Changed specimen requirements fro CAI, changed name of Dr. H Chan lab. Changed Fluoride to HICL. Changed Voltage Gated Calcium Channel to UBC. Sep 19 2017 Minor Jennifer Clarke Added Urine to all Prov Tox tests, added more prov Tox tests Sep 26 2017 Minor Jennifer Clarke Changed test site for Thallium, urine Oct 17 2017 Minor Jennifer Clarke Made MPFTV no longer available. Added FTVUL, PTHI comment for MSJ Oct 20 2017 Minor Jennifer Clarke Added VEGF-D Dec 12 2017 Minor Jennifer Clarke Changed C3 to frozen. Made Meat fibres, Listeria and Stool Eosinophils unavailable Dec 14 2017 Minor Jennifer Clarke Removed TG by MS as separate test. Added independent growth EPO to stem cell description, Blueprint genetics (no kit). Changed tube type on some mitogen tests. Changed fasting lipid comments Dec 18 2017 Minor Jennifer Clarke Changed storage and transport of Fecal Calprotectin to frozen Dec 27 2017 Minor Jennifer Clarke Made unavailable myoglobin, KETP, urobilinogen, OBG, FATU Feb 01 2018 Minor Jennifer Clarke Added Creatinine clearance, removed ATPOP comment from TG, added new BCCDC website, added price to VEGF-D, changed fasting comments as per Dr. S Wong. May 04 2018 Minor Jennifer Clarke TBQS-no approval, added INFg, IL-12, QHBSAG, reinstated KETP for MSJ, changed the location of the Mitogen Labs req, changed the location of HESFLO, removed Lupus from DSDNA, added Li or Na to all DRK GRN, changed dose time requirements for CSA, CSA2 and TACR, added BCCH for <19 years of age for CMV, EBV and PPOLY, added Dr. Merkley, changed sample type for FEP May 09 2018 Minor Jennifer Clarke Changed RBC Mg information, Cortisol storage, AATGPP sample requirements, methotrexate sendouts, Aldosterone sample type and added AICBSB May 10 2018 Minor Jennifer Clarke Added/changed information on San Filippo A,C,D and Amino Acids, Urine and Sulfatides urine, changed AATS to CLS May 17 2018 Minor Jennifer Clarke Changed Phosphatidylserine from BCCH to Mitogen, CW codes for Viral loads, virology updates May 22 2018 Minor Jennifer Clarke Virology and BCTox updates May 24 2018 Minor Jennifer Clarke Changed AATS address to UAH, BC Tox updates

Division of Pre and Post Examination, Page 285 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018

Hard Copy Distribution Log: Location(s) Archived Obsolete version, replaced with Current Date version by: Do not print hard copy. Emergency copy can be obtained from Accessioning Technical Coordinator’s computer C:\Test Directory\SPH Test Directory.pdf

Division of Pre and Post Examination, Page 286 of 286 Providence Health Care, Vancouver B.C., Canada Approved by: Dr. M. Trotter

PHC Test Directory Revision date: May 24, 2018