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Modernizing Algae Cultivation Practices to Enhance Applications As Nutritional Products BIO, Montreal, July 25Th 2017 Algenol Overview

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Modernizing Algae Cultivation Practices to Enhance Applications As Nutritional Products BIO, Montreal, July 25Th 2017 Algenol Overview Modernizing Algae Cultivation Practices to Enhance Applications as Nutritional Products BIO, Montreal, July 25th 2017 Algenol Overview Algenol Biotech LLC is an industrial biotech company developing biobased products utilizing algae (founded in 2006) HQ, R&D and manufacturing in Fort Myers, FL R&D facility in Berlin, Germany Operates in multiple sectors AgTech & Food applications Algae based proteins o Natural colorants o Proteins for human and animal nutrition Soil treatment o Biostimulants o Biofertilizers Contract research, development and manufacturing Algal product research and development Synthetic biology: developing cyanobacteria as heterologous expression systems Photobioreactor-based algae production tolling Ethanol and biocrude – through synthetic biology 2 Algae as a platform meeting global needs Biofuels and Agro Premium Feed Food Nutraceuticals Biocrude Sciences Cosmetics ($380 B) ($560 B) ($11 B) ($1,800 B) ($150 B) ($80 B) • Biocrude • Fish feed • Bio-fertilizers • Oil, fat and • Anti-aging • Single cell • Bio-gasoline • Livestock • Biostimulants protein • Natural and proteins • Biobased feed • Soil replacement organic skin • Carotenoids diesel reclamation • Food care and pigments • Renewable • Waste water Ingredients • Bioactive jet fuel treatment compounds • Ethanol ALGAE HAS CHARACTERISTICS SUITABLE FOR A WIDE RANGE OF PRODUCTS Source: Bloomberg New Energy Finance, Advanced Transport Research Note, June 2015. 3 Algae Nutraceuticals Algae Nutraceuticals Carotenoids and Bioactive Single Cell Protein Pigments Compounds Spirulina Beta Carotene Antioxidants Lipids Chlorella Astaxanthin Carbohydrates Lutein Peptides Lycopene Proteins Zeaxanthin Vitamins Cholorophyll Natural Colorants Fucoxanthin Canthaxanthin ALGAE NUTRACEUTICALS DRIVEN BY HEALTH BENEFITS, TREND TOWARD “NATURAL” FOOD Source: Comprehensive Report on Attractive Algae Product Opportunities Preview (Feb. 2015) 4 Algae Agroscience products Algae for Agro Sciences Soil Treatment Wastewater ($60 B) treatment ($90 B) Biofertilizers & Fertilizer runoff Biostimulants reclamation Sewage/wastewater Soil reclamation treatment Biofilters for fish pond Carbon sequestration effluents ALGAE IN AG SCIENCES DRIVEN BY YIELDS, SUSTAINABILITY CONCERNS & ORGANIC FARMING 5 Algenol Core Technology Algenol can enable production of multiple algae based products Natural Colorants Food, beverage, cosmetics Protein for Nutraceuticals nutrition Phycocyanin, Vegan, excellent spirulina amino acid profile Soil Personal Care Treatment Ingredients Active fertilizer Functional and ingredients, active biostimulants Oil based products Omega 3s 6 PBR Array 7 Fort Myers, FL The Algenol Vertical Photobioreactor (PBR) Each PBR maximizes light distribution and moderates temperature for maximum yields Low cost systems open up profitable System eliminates contamination from dirt, large-scale debris, bird feces, fly larvae and other unwanted cultivation substances PBRs efficiently deliver carbon dioxide and nutrients to the algal culture PBRs allow tight control over algal culture Enclosed production conditions permitting better control over desired systems industrialize algae composition algae cultivation PBRs limit contamination from other algae species to maintain monoculture conditions Greater product concentrations at harvest compared to open ponds System automation reduces labor costs Highly efficient algae growth Proven effectiveness across broad range of algae types 8 An Industrial Production System How we industrialized Algae cultivation Criterion Open Pond Traditional Algenol PBR Algenol PBR Advantages Technology PBR cultivation technology system Capital Costs Low Very High Medium Low cost PBRs, optimized for commercial ops Product Quality Variable High High Improved product consistency due to reduced risk of contaminating bacteria, algae and toxins Productivity Low to Medium High High At least 2X open pond productivity, due to higher volumetric and area production rates Scalability High Very Low High Algenol PBRs suitable and designed for large industrial operations Ability to handle wide High Low Medium to High Proven ability to handle different types of algae range of algae Ability to handle Low High High Approved GE algae; easier permitting genetically engineered (GE) algae Manage yields of Low High High Can configure PBR system conditions to components optimize specific components Contamination control Low High High Self-contained system with optimized clean-up procedures and low risk pf process upsets Precise process control Low to Medium High High Precise control of flow, air & CO2 diffusion Sustainability Medium to High High High Lower water and CO2 losses. Low carbon footprint. 9 Productivity Advantages of Vertical PBR Arrays Efficient light utilization Very Low CO2 losses Constrained CO2, Water light utilization losses Competing & predator organisms High exposure layer Dark area Low Culture stress VERTICAL PBR ARRAYS TRADITIONAL OPEN PONDs • Higher Productivity • Sustainability • Competitive costs Better light management Less fresh water use Lower total lifecycle costs Reduced competing organisms Improved nutrient utilization Higher product quality Better gas management Better CO2 utilization State-of-the-art PBRs 10 PBR Array Measurements and Controls Fully automated systems allow precise process control 11 Business Focus: Natural Colorants . A spirulina algae strain (Arthrospira platensis) is exempt from the US Food and Drug Administration (FDA) certification . Firm regulatory footing in the US, EU and Japan . $2 billion/7% annual growth market with strong positive drivers – high profit potential . Spirulina contains protein based phycocyanin pigments, which provide a vivid blue color . DIC-Earthrise, Parry Nutraceuticals and a few Chinese spirulina producers already sell phycocyanin produced by cultivating and harvesting open ponds . Algenol will produce cost competitive phycocyanin using commercially-proven, clean, controlled and highly productive photobioreactors 12 Business Focus: Proteins • Protein market has huge potential for large-scale expansion of our business • Potential $15 Billion market for human nutrition alone • Spirulina has a “Super Food” profile, which consumers find attractive • Spirulina includes important amino-acids that vegetable proteins are deficient in • Spirulina’s regulatory status and consumer acceptance are important factors • Algenol production platform offers potential to deliver rich nutritional value of algae proteins at competitive cost while providing process traceability and control • Multiple valuable byproducts from the production of protein Protein % w/w 70 60 50 40 30 20 10 0 Protein % w/w 13 Synthetic Biology Capabilities Extensive experience in development of cyanobacteria as heterologous expression systems Proteins, enzymes and biochemicals Ethanol and higher alcohols, biocrude Targeted Approaches Introductions or modifications to known genes expected or intended to result in a specific improvement to strain Non-Targeted Approaches Screens, selections, and adaptation to identify strains with improved properties Mutagens can be used to increase genetic diversity Transposon mutagenesis allow quick gene ID and full segregation Additional traits can be added to improved strain With modern ‘omics technologies, this is a powerful method for gene discovery Complete molecular tool box for multiple strains Plasmid vector construction Conjugation and transformation Homologous recombination Trait stacking 14 Algenol One Stop Shop for Commercialization Algae Algae Algae Algae Process Commercial Regulatory R&D Development Production Approvals Key Capabilities: • 30,000 ft2 labs • Lab process • Large-scale • Experience with • Very large algal strain development outdoor process State and Federal collection capabilities scale-up regulations • Lab based algae • 4.5 acre Process Dev. • High performance • Toxicology screening capability Unit (PDU) PBR Design and screening • Synthetic Biology • Analytical chemistry Manufacturing • NEPA on 36-acres • Licensed site Fort Myers site Key Activities: • Lab screening, • Test multiple strains in • Test commercial • Secure permits for photobiology, lab outside conditions designs at pre- GE algae, e.g. cultivation • Optimize PBRs and commercial scale MCAN, state permits • Develop/improve cultivation procedures • Secure product • Secure all algae strains • Develop commercial approvals and environmental • Analyze cellular process designs certifications permits for site constituents • Perform Product QC • FDA “game plan” • Identification of best • Final designs • MCANs for new Key Deliverables: • Validation of economics strains to meet • Preliminary designs • On-spec commercial strains. commercial specs. • Pre-commercial samples batches • Regulatory approval 15 The Algenol Advantage • Highly productive and sustainable algae production platforms • Ability to develop all natural products and customize them for individual clients • State-of-the-art laboratory facilities – United States and Germany • Advanced in-house analytics • Dedicated, high tech cultivation rooms • Integrated biology and engineering R&D • Highly skilled and specialized scientists For more information, please contact us: Jacques Beaudry-Losique, CBO [email protected] 703-389-0690 16.
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