Modernizing Algae Cultivation Practices to Enhance Applications as Nutritional Products BIO, Montreal, July 25th 2017 Algenol Overview
Algenol Biotech LLC is an industrial biotech company developing biobased products utilizing algae (founded in 2006)
HQ, R&D and manufacturing in Fort Myers, FL
R&D facility in Berlin, Germany
Operates in multiple sectors
AgTech & Food applications
Algae based proteins o Natural colorants o Proteins for human and animal nutrition
Soil treatment o Biostimulants o Biofertilizers
Contract research, development and manufacturing
Algal product research and development
Synthetic biology: developing cyanobacteria as heterologous expression systems
Photobioreactor-based algae production tolling
Ethanol and biocrude – through synthetic biology
2 Algae as a platform meeting global needs
Biofuels and Agro Premium Feed Food Nutraceuticals Biocrude Sciences Cosmetics ($380 B) ($560 B) ($11 B) ($1,800 B) ($150 B) ($80 B)
• Biocrude • Fish feed • Bio-fertilizers • Oil, fat and • Anti-aging • Single cell • Bio-gasoline • Livestock • Biostimulants protein • Natural and proteins • Biobased feed • Soil replacement organic skin • Carotenoids diesel reclamation • Food care and pigments • Renewable • Waste water Ingredients • Bioactive jet fuel treatment compounds • Ethanol
ALGAE HAS CHARACTERISTICS SUITABLE FOR A WIDE RANGE OF PRODUCTS
Source: Bloomberg New Energy Finance, Advanced Transport Research Note, June 2015.
3 Algae Nutraceuticals
Algae Nutraceuticals
Carotenoids and Bioactive Single Cell Protein Pigments Compounds
Spirulina Beta Carotene Antioxidants Lipids Chlorella Astaxanthin Carbohydrates Lutein Peptides Lycopene Proteins Zeaxanthin Vitamins Cholorophyll
Natural Colorants
Fucoxanthin
Canthaxanthin ALGAE NUTRACEUTICALS DRIVEN BY HEALTH BENEFITS, TREND TOWARD “NATURAL” FOOD
Source: Comprehensive Report on Attractive Algae Product Opportunities Preview (Feb. 2015)
4 Algae Agroscience products
Algae for Agro Sciences
Soil Treatment Wastewater ($60 B) treatment ($90 B)
Biofertilizers & Fertilizer runoff Biostimulants reclamation
Sewage/wastewater Soil reclamation treatment
Biofilters for fish pond Carbon sequestration effluents
ALGAE IN AG SCIENCES DRIVEN BY YIELDS, SUSTAINABILITY CONCERNS & ORGANIC FARMING
5 Algenol Core Technology
Algenol can enable production of multiple algae based products
Natural Colorants Food, beverage, cosmetics
Protein for Nutraceuticals nutrition Phycocyanin, Vegan, excellent spirulina amino acid profile
Soil Personal Care Treatment Ingredients Active fertilizer Functional and ingredients, active biostimulants
Oil based products Omega 3s
6 PBR Array
7 Fort Myers, FL The Algenol Vertical Photobioreactor (PBR)
Each PBR maximizes light distribution and moderates temperature for maximum yields Low cost systems open up profitable System eliminates contamination from dirt, large-scale debris, bird feces, fly larvae and other unwanted cultivation substances
PBRs efficiently deliver carbon dioxide and nutrients to the algal culture
PBRs allow tight control over algal culture Enclosed production conditions permitting better control over desired systems industrialize algae composition algae cultivation PBRs limit contamination from other algae species to maintain monoculture conditions
Greater product concentrations at harvest compared to open ponds
System automation reduces labor costs Highly efficient algae growth Proven effectiveness across broad range of algae types
8 An Industrial Production System How we industrialized Algae cultivation
Criterion Open Pond Traditional Algenol PBR Algenol PBR Advantages Technology PBR cultivation technology system Capital Costs Low Very High Medium Low cost PBRs, optimized for commercial ops
Product Quality Variable High High Improved product consistency due to reduced risk of contaminating bacteria, algae and toxins Productivity Low to Medium High High At least 2X open pond productivity, due to higher volumetric and area production rates Scalability High Very Low High Algenol PBRs suitable and designed for large industrial operations Ability to handle wide High Low Medium to High Proven ability to handle different types of algae range of algae Ability to handle Low High High Approved GE algae; easier permitting genetically engineered (GE) algae Manage yields of Low High High Can configure PBR system conditions to components optimize specific components Contamination control Low High High Self-contained system with optimized clean-up procedures and low risk pf process upsets
Precise process control Low to Medium High High Precise control of flow, air & CO2 diffusion
Sustainability Medium to High High High Lower water and CO2 losses. Low carbon footprint.
9 Productivity Advantages of Vertical PBR Arrays
Efficient light utilization Very Low CO2 losses Constrained CO2, Water light utilization losses
Competing & predator organisms
High exposure layer
Dark area Low Culture stress VERTICAL PBR ARRAYS TRADITIONAL OPEN PONDs
• Higher Productivity • Sustainability • Competitive costs Better light management Less fresh water use Lower total lifecycle costs Reduced competing organisms Improved nutrient utilization Higher product quality Better gas management Better CO2 utilization State-of-the-art PBRs
10 PBR Array Measurements and Controls
Fully automated systems allow precise process control
11 Business Focus: Natural Colorants
. A spirulina algae strain (Arthrospira platensis) is exempt from the US Food and Drug Administration (FDA) certification . Firm regulatory footing in the US, EU and Japan . $2 billion/7% annual growth market with strong positive drivers – high profit potential . Spirulina contains protein based phycocyanin pigments, which provide a vivid blue color . DIC-Earthrise, Parry Nutraceuticals and a few Chinese spirulina producers already sell phycocyanin produced by cultivating and harvesting open ponds . Algenol will produce cost competitive phycocyanin using commercially-proven, clean, controlled and highly productive photobioreactors
12 Business Focus: Proteins
• Protein market has huge potential for large-scale expansion of our business • Potential $15 Billion market for human nutrition alone • Spirulina has a “Super Food” profile, which consumers find attractive • Spirulina includes important amino-acids that vegetable proteins are deficient in • Spirulina’s regulatory status and consumer acceptance are important factors • Algenol production platform offers potential to deliver rich nutritional value of algae proteins at competitive cost while providing process traceability and control • Multiple valuable byproducts from the production of protein Protein % w/w 70 60 50 40 30 20 10 0
Protein % w/w
13 Synthetic Biology Capabilities
Extensive experience in development of cyanobacteria as heterologous expression systems
Proteins, enzymes and biochemicals
Ethanol and higher alcohols, biocrude
Targeted Approaches
Introductions or modifications to known genes expected or intended to result in a specific improvement to strain
Non-Targeted Approaches
Screens, selections, and adaptation to identify strains with improved properties
Mutagens can be used to increase genetic diversity
Transposon mutagenesis allow quick gene ID and full segregation
Additional traits can be added to improved strain
With modern ‘omics technologies, this is a powerful method for gene discovery
Complete molecular tool box for multiple strains
Plasmid vector construction
Conjugation and transformation
Homologous recombination
Trait stacking
14 Algenol One Stop Shop for Commercialization
Algae Algae Algae Algae Process Commercial Regulatory R&D Development Production Approvals
Key Capabilities: • 30,000 ft2 labs • Lab process • Large-scale • Experience with • Very large algal strain development outdoor process State and Federal collection capabilities scale-up regulations • Lab based algae • 4.5 acre Process Dev. • High performance • Toxicology screening capability Unit (PDU) PBR Design and screening • Synthetic Biology • Analytical chemistry Manufacturing • NEPA on 36-acres • Licensed site Fort Myers site Key Activities: • Lab screening, • Test multiple strains in • Test commercial • Secure permits for photobiology, lab outside conditions designs at pre- GE algae, e.g. cultivation • Optimize PBRs and commercial scale MCAN, state permits • Develop/improve cultivation procedures • Secure product • Secure all algae strains • Develop commercial approvals and environmental • Analyze cellular process designs certifications permits for site constituents • Perform Product QC • FDA “game plan” • Identification of best • Final designs • MCANs for new Key Deliverables: • Validation of economics strains to meet • Preliminary designs • On-spec commercial strains. commercial specs. • Pre-commercial samples batches • Regulatory approval
15 The Algenol Advantage
• Highly productive and sustainable algae production platforms • Ability to develop all natural products and customize them for individual clients • State-of-the-art laboratory facilities – United States and Germany • Advanced in-house analytics • Dedicated, high tech cultivation rooms • Integrated biology and engineering R&D • Highly skilled and specialized scientists
For more information, please contact us:
Jacques Beaudry-Losique, CBO [email protected] 703-389-0690
16