Predominance of Bacillus Sp. in Soil Samples of the Southern Regions of Western Ghats, India

Total Page:16

File Type:pdf, Size:1020Kb

Predominance of Bacillus Sp. in Soil Samples of the Southern Regions of Western Ghats, India Ann Microbiol (2015) 65:431–441 DOI 10.1007/s13213-014-0876-1 ORIGINAL ARTICLE Predominance of Bacillus sp. in soil samples of the southern regions of Western Ghats, India Gowdaman Vasudevan & Venkatachalam Siddarthan & Prabagaran Solai Ramatchandirane Received: 5 August 2013 /Accepted: 12 March 2014 /Published online: 17 April 2014 # Springer-Verlag Berlin Heidelberg and the University of Milan 2014 Abstract The aim of this study was to determine the bacterial samples assessed by both cultivation-dependent and diversity in soils of the southern region of the Western Ghats, a cultivation-independent methods. ‘biodiversity hotspot’, and thereby futher our understanding of the microbial communities in this ecological niche. The Keywords Western Ghats . Diversity index . 16S rRNA . diversity and phylogeny of bacterial populations in soil sam- ARDRA . Metagenomic DNA . Bacillus species ples collected from various locations of the Tamil Nadu and Kerala regions of Western Ghats were compared using both cultivation-dependent and cultivation-independent methods. Introduction A total of 171 bacterial strains were isolated based on their morphological characteristics and their diversity indices cal- India is considered by UNESCO (2012) to have two of the culated. The distinctive amplified ribosomal DNA restriction ‘mega diversity hotspots’ in the world, namely, the Himalayas analysis (ARDRA) pattern of each isolate was determined, and the Western Ghats. The Western Ghats is a forested tract and representative isolates were then subjected to 16S rRNA of relatively smooth but very old mountain range that extends gene sequencing. On the basis of their sequence similarity, the from Central Maharashtra to the southern tip of Kerala. The isolates were distributed among three different genera belong- region supports a wide range of endemic taxa with nearly ing to Firmicutes (83.3 %), Proteobacteria (8.3 %) and high 1,500 species of flowering plants (38 %), 126 species of G+C Gram-positive bacteria (8.3 %). The highest and the amphibians (78 %), 157 species of reptiles (62 %), 508 species lowest values for the diversity indices were obtained for of birds (4 %) and 137 species of mammals (12 %) identified metagenomic DNA extracted from isolates BWGA and to date (Bawa et al. 2007). The diversity of flora and fauna of BVP, respectively; these were used for 16S rRNA gene library the Western Ghats has been reasonably well documented, but construction and analysis. Based on their phylogenetic analy- rather fewer studies have examined fungal diversity in the area sis, the predominant members of the habitat were found to (Raviraja 2005) and fewer yet have looked at the diversity of belong to the phylum Firmicutes (84.62 %). Firmicutes was bacteria. Soil bacteria are an essential component of the biotic the dominant bacterial phylum detected by both approaches, community in natural forests and are largely responsible for but the culture-independent approach detected a considerably ecosystem functioning due to their participation in most nu- higher number of uncultivable bacteria. In conclusion, in our trient transformations (Hackl et al. 2004). Although the main study of the bacterial diversity of this Western Ghats region, diversity of life has been proven to be microbial, the vast we fund that the genus Bacillus was predominant among the majority of soil bacteria still remain unidentified because only a minor percentage of naturally occurring microorganisms can be cultured (Pace 1997). Electronic supplementary material The online version of this article The diversity of microorganisms and the role they play ‘in (doi:10.1007/s13213-014-0876-1) contains supplementary material, ’ which is available to authorized users. situ remains largely unknown. For almost a century, culture- dependent methods used to study bacterial diversity have been : : * G. Vasudevan V. Siddarthan P. Solai Ramatchandirane ( ) continuously optimized to detect diverse microorganisms. Molecular Microbiology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India However, only a small proportion of the bacteria present in e-mail: [email protected] extreme environments are readily cultivable, limiting further, 432 Ann Microbiol (2015) 65:431–441 more detailed study. In addition, culture-dependent methods been not many reports on bacterial diversity. Therefore, the are selective and biased based on the type of media used, the aim of our study was to estimate bacterial diversity and nutrients provided and the culture conditions. This has led in identify the predominant soil bacteria in the southern regions more recent decades to the development of culture- of the Western Ghats using both culture-dependent and ad- independent methods to study the enormous diversity of un- vanced culture-independent methods in order to obtain an cultivable organisms and emphasized the need for comple- overview of the bacterial diversity. Although the culture- mentary approaches for the analysis of soil bacterial diversity independent approach is considered to be the superior tech- (Vaz-Moreira et al. 2011). nology for studying bacterial diversity, culture-dependent The relatively recent application of molecular methods to methods are considered to potentially be of more practical investigate unculturable microbes from diverse environments importance (Burmølle et al. 2009). has altered commonly accepted views of microbial diversity. Research based on the extraction of total community DNA from environmental samples followed by PCR, cloning and sequencing of 16S rRNA genes has become conventional, Materials and methods often comprising one of the first steps in the study of the bacterial diversity of an environment of importance (Oline Site description and sampling et al. 2006). The recovery and analysis of 16S rRNA genes directly from environmental DNA provides a means for in- The present-day Western Ghats constitute a continuous vestigating microbial populations in any habitat, eliminating chain of small to medium-sized mountain ranges running the dependence on the isolation of pure cultures (Amann et al. between 8 and 21°N latitude and 73 and 77 °E longitude. 1995b; Ahmad et al. 2009). The southern part of the Western Ghats region encom- Only limited investigations have been carried out on bac- passes the states of Tamil Nadu and Kerala, where many terial diversity in various forest ecosystems of the Western reserve forests can be found. Soil samples from 12 differ- Ghats. The southern regions of the Western Ghats are mostly ent sampling sites throughout the southern regions of located in the states of Tamil Nadu and Kerala. These two Western Ghats (Fig. 1) were obtained by first removing states are characterized by their abundance of forested areas, the top surface soil and then collecting samples at a soil which are largely unexplored to the fullest. To date, there have depth of about 10 cm. A small amount of each sample was Fig. 1 Experimental area depicting Western Ghats and the locations of the sampling sites Ann Microbiol (2015) 65:431–441 433 used for serial dilution plating, and the remaining was Sigma-Aldrich, St. Louis, MO). The ARDRA profiles of stored at 4 °C until further use. different isolates were compared manually and the observed DNA polymorphisms scored as dominant markers and con- Isolation and morphological characterization of bacteria verted to a binary matrix. The data were used to derive similarity measures in terms of Jaccord’s coefficient in all Enumeration of soil bacteria was achieved by serial dilu- possible pairwise combinations. The similarity matrix was tion and plating on different types of solid media at various used for cluster analysis using the UPGMA method in the strengths, such as nutrient agar (NA), yeast starch agar and NTSys-PC 2.1 software package (Exeter Publishing, soil extract agar (SEA) [see Electronic Supplementary Setauket, NY). Material (ESM) Table 1] (Bianchi and Armand 1982). Following incubation at 37 °C for 24–72 h, the isolates Extraction and purification of metagenomic DNA were selected based on their cell morphology, colony mor- phology, Gram reaction, among others, and the results Soil DNA was extracted from 5 g of each soil sample with documented. 13 ml of extraction buffer (100 mM Tris–HCl pH 8; 100 mM EDTA pH 8, 100 mM sodium phosphate, 1.5 M NaCl, 1 % Diversity analysis CTAB) and incubated for 30 min at 37 °C before being centrifuged at 6,000 rpm for 5 min. The supernatant was A range of diversity indices (ESM Table 2) were used to collected and used for DNA isolation as described by Zhou characterize the bacterial community studies, including the et al. (1996). Metagenomic DNA consists of humic acids and ubiquitous Shannon index, the evenness index derived from other phenolic compounds that inhibit its downstream appli- the former and Simpson’s dominance index and its associated cations. Hence, purification is important for further process- equitability index (McCaig et al. 1999;ChoandKim2000; ing. Purification was preceded by treatment with a modified Dunbar et al. 2000). method of Ausubel et al. (1987). Genotypic characterization of isolates Construction of library and sequence analysis Genomic DNA was isolated from bacterial cultures grown in Purified PCR products were ligated with pTZ57R/T vector Luria Bertani (LB) broth (Hi Media, Mumbai, India) using (Fermentas) following the manufacturer’s instructions. The standard protocols (Sambrook et al. 1989). The 16S rRNA ligation reaction
Recommended publications
  • The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio In
    microorganisms Review The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease Spase Stojanov 1,2, Aleš Berlec 1,2 and Borut Štrukelj 1,2,* 1 Faculty of Pharmacy, University of Ljubljana, SI-1000 Ljubljana, Slovenia; [email protected] (S.S.); [email protected] (A.B.) 2 Department of Biotechnology, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia * Correspondence: borut.strukelj@ffa.uni-lj.si Received: 16 September 2020; Accepted: 31 October 2020; Published: 1 November 2020 Abstract: The two most important bacterial phyla in the gastrointestinal tract, Firmicutes and Bacteroidetes, have gained much attention in recent years. The Firmicutes/Bacteroidetes (F/B) ratio is widely accepted to have an important influence in maintaining normal intestinal homeostasis. Increased or decreased F/B ratio is regarded as dysbiosis, whereby the former is usually observed with obesity, and the latter with inflammatory bowel disease (IBD). Probiotics as live microorganisms can confer health benefits to the host when administered in adequate amounts. There is considerable evidence of their nutritional and immunosuppressive properties including reports that elucidate the association of probiotics with the F/B ratio, obesity, and IBD. Orally administered probiotics can contribute to the restoration of dysbiotic microbiota and to the prevention of obesity or IBD. However, as the effects of different probiotics on the F/B ratio differ, selecting the appropriate species or mixture is crucial. The most commonly tested probiotics for modifying the F/B ratio and treating obesity and IBD are from the genus Lactobacillus. In this paper, we review the effects of probiotics on the F/B ratio that lead to weight loss or immunosuppression.
    [Show full text]
  • Distribution and Characteristics of Bacillus Bacteria Associated with Hydrobionts and the Waters of the Peter the Great Bay, Sea of Japan I
    ISSN 0026-2617, Microbiology, 2008, Vol. 77, No. 4, pp. 497–503. © Pleiades Publishing, Ltd., 2008. Original Russian Text © I.A. Beleneva, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 4, pp. 558–565. EXPERIMENTAL ARTICLES Distribution and Characteristics of Bacillus Bacteria Associated with Hydrobionts and the Waters of the Peter the Great Bay, Sea of Japan I. A. Beleneva1 Zhirmunskii Institute of Marine Biology, Far East Division, Russian Academy of Sciences, ul. Pal’chevskogo, 17, Vladivostok 690041, Russia Received May 28, 2007 Abstract—Bacilli of the species Bacillus subtilis, B. pumilus, B. mycoides, B. marinus and B. licheniformis (a total of 53 strains) were isolated from 15 invertebrate species and the water of the Vostok Bay, Peter the Great Bay, Sea of Japan. Bacilli were most often isolated from bivalves (22.7%) and sea cucumbers (18.9%); they occurred less frequently in sea urchins and starfish (13.2 and 7.5%, respectively). Most of bacilli strains were isolated from invertebrates inhabiting silted sediments. No Bacillus spp. strains were isolated from invertebrates inhabiting stony and sandy environments. The species diversity of bacilli isolated from marine objects under study was low. Almost all bacterial isolates were resistant to lincomycin. Unlike B. pumilus, B. subtilis isolates were mostly resistant to benzylpenicillin and ampicillin. Antibiotic sensitivity of B. licheniformis strains was variable (two strains were resistant to benzylpenicillin and oxacillin, while one was sensitive). A significant fraction of isolated bacilli contained pigments. Pigmented strains were more often isolated from seawater sam- ples, while colorless ones predominated within hydrobionts. B. subtilis colonies had the broadest range of co- lors.
    [Show full text]
  • Chapter 11: Bacteria Bacterial Groups
    Bacterial Groups u Most widely accepted taxonomic classification for bacteria is Bergey’s Manual of Systematic Bacteriology. u 5000 bacterial species identified, 3100 classified. Chapter 11: Bacteria u Bacteria are divided into four divisions (phyla) according to the characteristics of their cell walls. u Each division is divided into sections according to: u Gram stain reaction u Cell shape u Cell arrangements u Oxygen requirements u Motility u Nutritional and metabolic properties u Each section contains several genera. Four Divisions of Bacteria Classification of Bacteria Procaryotes Gram-Negative Division II Wall-Less Archaea Bacteria Bacteria Bacteria Bacteria (Gracilicutes) (Firmicutes) (Tenericutes) (Mendosicutes) Thin Cell Walls Thick cell Walls Lack cell walls Unusual cell walls Division I. Gram-Negative Bacteria Gram Negative Bacteria Spirochetes 1. Spirochetes u Helical shape. Flexible. u Contain two or more axial filaments (endoflagella). u Move in corkscrew pattern. u Medically important members: F Treponema pallidum: Syphilis F Borrelia spp.: Lyme disease, relapsing fever F Leptospira: Leptospirosis 1 Syphilis is Caused by a Spirochete Lyme Disease is Caused by a Spirochete Primary syphilitic chancre and secondary rash. Source: Tropical Medicine and Parasitology, 1997 Lyme Disease early lesion at tick bite site. Source: Medical Microbiology, 1998 2. Aerobic, Motile, Helical/Vibroid Gram- Negative Bacteria Gram Negative Bacteria u Rigid helical shape or curved rods. Aerobic, Motile, Helical/Vibroid u Lack axial filaments (endoflagella); have polar Gram-Negative Bacteria flagella instead. u Most are harmless aquatic organisms. u Genus Azospirillum fixes nitrogen in soil. u Genus Bdellovibrio attacks other bacteria. u Important pathogens include: F Campylobacter jejuni: Most common bacterial food- borne intestinal disease in the United States (2 million cases/year).
    [Show full text]
  • Technical Method
    J Clin Pathol: first published as 10.1136/jcp.38.9.1073 on 1 September 1985. Downloaded from J Clin Pathol 1985;38:1073-1084 Technical method Use of microwaves for acid and alcohol fast staining S HAFIZ, RC SPENCER, MARGARET LEE, organism depends on several factors but remains an HILARY GOOCH, BI DUERDEN Department important component in the identification of a ofMedical Microbiology, Sheffield University Medi- restricted group of organisms. cal School, Sheffield The technical problems associated with the classic Ziehl-Neelsen method include the dangers of heat- ing by flaming torch in laboratories in which volatile Certain bacteria that are characterised by a high organic solvents are used, the deposits that accumu- content of lipid in their cell walls cannot be stained late on the underside of the slide as a result of heat- by simple stains, and either heat or prolonged con- ing, and the crystalline deposits of stain that collect tact is required to drive the stain into the cells; once on the film as a result of evaporation and drying. stained they resist decolourisation by mineral acids This method also requires 20-30 minutes of or acid and alcohol. These organisms are designated laboratory time. Numerous minor modifications acid fast or acid and alcohol fast. They include have been made to the Ziehl-Neelsen method by Mycobacterium tuberculosis and related mycobac- increasing the concentration of carbolic magenta or teria, Mycobacterium leprae, and certain of the by cold staining techniques,'0-'2 but the basic prob- actinomycetes. Koch first stained the tubercle bacil- lems remain. lus by immersion in an alkaline solution of Some of the problems may be overcome by the copyright.
    [Show full text]
  • Bacteria Associated with Larvae and Adults of the Asian Longhorned Beetle (Coleoptera: Cerambycidae)1
    Bacteria Associated with Larvae and Adults of the Asian Longhorned Beetle (Coleoptera: Cerambycidae)1 John D. Podgwaite2, Vincent D' Amico3, Roger T. Zerillo, and Heidi Schoenfeldt USDA Forest Service, Northern Research Station, Hamden CT 06514 USA J. Entomol. Sci. 48(2): 128·138 (April2013) Abstract Bacteria representing several genera were isolated from integument and alimentary tracts of live Asian longhorned beetle, Anaplophora glabripennis (Motschulsky), larvae and adults. Insects examined were from infested tree branches collected from sites in New York and Illinois. Staphylococcus sciuri (Kloos) was the most common isolate associated with adults, from 13 of 19 examined, whereas members of the Enterobacteriaceae dominated the isolations from larvae. Leclercia adecarboxylata (Leclerc), a putative pathogen of Colorado potato beetle, Leptinotarsa decemlineata (Say), was found in 12 of 371arvae examined. Several opportunistic human pathogens, including S. xylosus (Schleifer and Kloos), S. intermedius (Hajek), S. hominis (Kloos and Schleifer), Pantoea agglomerans (Ewing and Fife), Serratia proteamaculans (Paine and Stansfield) and Klebsiella oxytoca (Fiugge) also were isolated from both larvae and adults. One isolate, found in 1 adult and several larvae, was identified as Tsukamurella inchonensis (Yassin) also an opportunistic human pathogen and possibly of Korean origin .. We have no evi­ dence that any of the microorganisms isolated are pathogenic for the Asian longhorned beetle. Key Words Asian longhorned beetle, Anaplophora glabripennis, bacteria The Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) a pest native to China and Korea, often has been found associated with wood- packing ma­ terial arriving in ports of entry to the United States. The pest has many hardwood hosts, particularly maples (Acer spp.), and currently is established in isolated popula­ tions in at least 3 states- New York, NJ and Massachusetts (USDA-APHIS 201 0).
    [Show full text]
  • Araştırma Isolation and Characterization of Bacillus
    Akademik Ziraat Dergisi 7(1):21-28 (2018) Araştırma ISSN: 2147-6403 DOI: http://dx.doi.org/10.29278/azd.440586 (Research) Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs Hasan Murat AKSOY1, Celal TUNCER1, Islam SARUHAN1, Ismail ERPER1, Murat OZTURK1, Izzet AKCA1 1Ondokuz Mayis University, Faculty of Agriculture, Department of Plant Protection, SAMSUN , Kabul tarihi 06 Nisan 2018 Sorumlu yazar: , e-posta:[email protected] Alınış tarihi: 19 Aralık 2017 İslam SARUHAN Abstract Ölü pentatomidlerden Bacillus megaterium Bacillus megaterium isolates, were demonstrated to izolatlarının izolasyonu, karekterizasyonu ve be efficient biocontrol agents against the green Palomena prasina nimflerine insektisital etkisi shield bug (Palomena prasina Pentatomidae). Firstly hazelnut orchards were Öz surveyed and four B. megateriumL., isolatesHeteroptera: were Bacillus megaterium obtained from P. prasina. The morphological, Palomena prasina physiological and biochemical characteristics of B. Bu çalışma, izolatlarının fındık megaterium isolates were determined according to kokarcasına ( L., Heteroptera: the standardized methodology. Additionally 16S Pentatomidae) karşıP. etkinprasina biyokontrol Bacillusajanları to megateriumolduğu gösterilmiştir. Öncelikle fındıkB. bahçelerindemegaterium survey yapılarak, 'dan dört rRNA gene sequence analyse was performed gene confirmed that isolates Sa-1, Sa-5, SAk-2 and izolatı elde edilmiştir. determine the isolates. Analysis of the 16S rRNA SAkc-19 are B. megaterium izolatlarının morfolojik, fizyolojik ve biyokimyasal homology to the type strains of B. megaterium. özellikleri standart tanı yöntemlerine göre Effectiveness of these isolates, withwas tested100% againstsequence P. sonucu;belirlenmiştir. Sa-1, Sa Ayrıca-5, SAk izolatların-2 ve SAkc tanısı- için 16S rRNAB. prasina nymphs in laboratory, at 25±1°C and 70±5 megateriumgen dizi analizi yapılmıştır.
    [Show full text]
  • Bacillus Subtilis: Model Organism for Cellular Development, and Industrial Workhorse
    MICROBE PROFILE Errington and Aart, Microbiology 2020;166:425–427 DOI 10.1099/mic.0.000922 Microbe Profile: Bacillus subtilis: model organism for cellular development, and industrial workhorse Jeffery Errington* and Lizah T van der Aart Graphical abstract Life cycle, environmental importance and industrial applications of B. subtilis. DNA and life cycle: the laboratory strain of B. subtilis is naturally transformable and, in the typical example illustrated, a foreign DNA segment ‘insert’ is integrated into the amyE genetic locus by double crossover homologus recombination. A crucial facet of the life cycle of most B. subtilis and most Firmicutes is their ability to switch from a classical binary fission, with equal segregation of sister chromosomes,to endospore formation. The resultant asymmetrical division generates small prespore (red) and larger mother- cell (green) compartments with different patterns of transcription. The tough endospore that results can remain dormant for a long period of time before germinating to resume vegetative growth. Environmental interactions: B. subtilis is typically found in association with plants as both an epiphyte and also within the rhizosphere. In some parts of the world batches of spores are used extensively for plant protection in the form of a seed dressing. B. subtilis has also been studied extensively as a model system for biofilm formation, switching classically between planktonic and sessile states. Industrial applications: B. subtilis and closely related organisms are responsible for huge levels of production of hydrolytic commodity enzymes, particularly proteases and amylases. They are also popular in probiotic formulations and can be engineered for production of fine chemicals, such as the vitamin, riboflavin.
    [Show full text]
  • Engineering Bacillus Megaterium for Production of Functional Intracellular Materials Katrin Grage1, Paul Mcdermott2 and Bernd H
    Grage et al. Microb Cell Fact (2017) 16:211 DOI 10.1186/s12934-017-0823-5 Microbial Cell Factories RESEARCH Open Access Engineering Bacillus megaterium for production of functional intracellular materials Katrin Grage1, Paul McDermott2 and Bernd H. A. Rehm3* Abstract Background: Over the last 10–15 years, a technology has been developed to engineer bacterial poly(3-hydroxybu- tyrate) (PHB) inclusions as functionalized beads, for applications such as vaccines, diagnostics and enzyme immobili- zation. This has been achieved by translational fusion of foreign proteins to the PHB synthase (PhaC). The respective fusion protein mediates self-assembly of PHB inclusions displaying the desired protein function. So far, beads have mainly been produced in recombinant Escherichia coli, which is problematic for some applications as the lipopolysac- charides (LPS) co-purifed with such inclusions are toxic to humans and animals. Results: In this study, we have bioengineered the formation of functional PHB inclusions in the Gram-positive bac- terium Bacillus megaterium, an LPS-free and established industrial production host. As B. megaterium is a natural PHB producer, the PHB-negative strain PHA05 was used to avoid any background PHB production. Plasmid-mediated T7 promoter-driven expression of the genes encoding β-ketothiolase (phaA), acetoacetyl-CoA-reductase (phaB) and PHB synthase (phaC) enabled PHB production in B. megaterium PHA05. To produce functionalized PHB inclusions, the N- and C-terminus of PhaC was fused to four and two IgG binding Z-domains from Staphylococcus aureus, respectively. The ZZ-domain PhaC fusion protein was strongly overproduced at the surface of the PHB inclusions and the cor- responding isolated ZZ-domain displaying PHB beads were found to purify IgG with a binding capacity of 40–50 mg IgG/g beads.
    [Show full text]
  • Selection, Identification and Optimization of the Growth Water Probiotic Consortium of Mangrove Ecosystems As Bioremediation and Biocontrol in Shrimp Ponds
    Selection, Identification and Optimization of The Growth, Setyati et al. JPHPI 2014, Volume 17 Nomor 3 Selection, Identification and Optimization of the Growth Water Probiotic Consortium of Mangrove Ecosystems as Bioremediation and Biocontrol in Shrimp Ponds Wilis Ari Setyati*1, Erni Martani², Triyanto², Subagiyo³, Muhammad Zainuddin⁴ 1) Departement of Marine Science, Faculty of Fisheries and Marine Science Diponegoro University, Post-graduate Student, Gadjah Mada University 2)Departement of Biotechnology, Post-graduate, Gadjah Mada University. ³)Marine Science Laboratory, Marine Science Departement, Diponegoro University. ⁴)Natural Product Laboratory, UPT (Integrated Laboratory), Diponegoro University. Faculty of Fisheries and Marine – Tembalang – Semarang Telp 024 7474698 *Coresponding author: [email protected] Accepted April 15th, 2014/Approved Agustus 10th, 2014 Abstract Shrimp aquaculture is an activity that potentially generates organic waste. The accumulation of organic matter is becoming one of the main factors causing the emergence of disease. Problem-solving approach that is most effective is through bioremediation. The aims of this study were to select, identify and cultivate bacteria from mangrove sediments from Cilacap, Rembang and Banyuwangi which potentially as probiotic consortium of bioremediation activity and biocontrol. The results showed that total of 45 isolates (proteolytic), 35 isolates (amylolytic), 35 isolates (lipolytic), and 18 isolates (cellulolytic). There were 59 bacterial isolates had antibacterial activity of vibrio (V. harveyi, V. alginolyticus, V. vulnificus and V. anguilarum). Based on the identification of 16 S-rRNA genes, 4 isolates showed that the C2 isolate was identified as Bacillus subtilis, C11 isolate was identified as Bacillus firmus, C13 and C14 isolates were identified as B. Flexus. This study concluded that cultivation of Bacillus subtilis C2 optimum at 2% molase and yeast extract 0.5% at pH 8 and 30 0C.
    [Show full text]
  • JMSCR Vol||06||Issue||12||Page 420-430||December 2018
    JMSCR Vol||06||Issue||12||Page 420-430||December 2018 www.jmscr.igmpublication.org Impact Factor (SJIF): 6.379 Index Copernicus Value: 79.54 ISSN (e)-2347-176x ISSN (p) 2455-0450 DOI: https://dx.doi.org/10.18535/jmscr/v6i12.65 Antimicrobial Assessment of Fresh Ripe and Dry Ripe Musa sapientum L. Peels against Selected Isolates Associated with Urinary Tract Infection in Port Harcourt, Nigeria Authors Konne, Felix Eedee, Nwokah, Easter Godwin, Wachukwu, Confidence Kinikanwo Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nkpolu - Oroworukwo P.M.B. 5080, Rivers State, Nigeria *Corresponding Author Konne, Felix Eedee Tel.: +2348035484367, Email: [email protected], [email protected] Abstract Banana peels is the outer coverage of the banana fruits commonly used to feed animals, fish and many others or thrown away as waste. Almost every part of a banana plant has medicinal values. Urinary Tract Infection is an infection that affects any parts of your urinary system (upper or lower). Usually its treatment and diagnosis are usually with antibiotics urine sample. Increase in bacterial resistant to conventional antibiotics has prompted the development of bacterial disease treatment strategies that are alternatives to conventional antibiotics. Aim: This study is to assess the antimicrobial properties of banana peels against selected isolates from Urinary Tract Infection sample. Methods: A Mid- stream urine samples collected from patients visiting BMSH with suspected cases of UTIs, were cultured. The isolates from culture was further analysis with agarose gel electrophoresis for the presence of 16SrRNA and Phylogenetic analysis shows Staphylococcus sciuri strain, a coagulase‐negative species, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis.
    [Show full text]
  • Screening of Antagonistic Bacterial Isolates from Hives of Apis Cerana in Vietnam Against the Causal Agent of American Foulbrood
    1202 Chiang Mai J. Sci. 2018; 45(3) Chiang Mai J. Sci. 2018; 45(3) : 1202-1213 http://epg.science.cmu.ac.th/ejournal/ Contributed Paper Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae Sasiprapa Krongdang [a,b], Jeffery S. Pettis [c], Geoffrey R. Williams [d] and Panuwan Chantawannakul* [a,e,f] [a] Bee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. [b] Interdisciplinary Program in Biotechnology, Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand. [c] USDA-ARS, Bee Research Laboratory, Beltsville, MD, 20705, USA. [d] Department of Entomology & Plant Pathology, Auburn University, Auburn, AL, 36849, USA. [e] Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. [f] International College of Digital Innovation, Chiang Mai University, 50200, Thailand. * Author for correspondence; e-mail: [email protected] Received: 15 February 2017 Accepted: 20 June 2017 ABSTRACT American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P.
    [Show full text]
  • The Genera Staphylococcus and Macrococcus
    Prokaryotes (2006) 4:5–75 DOI: 10.1007/0-387-30744-3_1 CHAPTER 1.2.1 ehT areneG succocolyhpatS dna succocorcMa The Genera Staphylococcus and Macrococcus FRIEDRICH GÖTZ, TAMMY BANNERMAN AND KARL-HEINZ SCHLEIFER Introduction zolidone (Baker, 1984). Comparative immu- nochemical studies of catalases (Schleifer, 1986), The name Staphylococcus (staphyle, bunch of DNA-DNA hybridization studies, DNA-rRNA grapes) was introduced by Ogston (1883) for the hybridization studies (Schleifer et al., 1979; Kilp- group micrococci causing inflammation and per et al., 1980), and comparative oligonucle- suppuration. He was the first to differentiate otide cataloguing of 16S rRNA (Ludwig et al., two kinds of pyogenic cocci: one arranged in 1981) clearly demonstrated the epigenetic and groups or masses was called “Staphylococcus” genetic difference of staphylococci and micro- and another arranged in chains was named cocci. Members of the genus Staphylococcus “Billroth’s Streptococcus.” A formal description form a coherent and well-defined group of of the genus Staphylococcus was provided by related species that is widely divergent from Rosenbach (1884). He divided the genus into the those of the genus Micrococcus. Until the early two species Staphylococcus aureus and S. albus. 1970s, the genus Staphylococcus consisted of Zopf (1885) placed the mass-forming staphylo- three species: the coagulase-positive species S. cocci and tetrad-forming micrococci in the genus aureus and the coagulase-negative species S. epi- Micrococcus. In 1886, the genus Staphylococcus dermidis and S. saprophyticus, but a deeper look was separated from Micrococcus by Flügge into the chemotaxonomic and genotypic proper- (1886). He differentiated the two genera mainly ties of staphylococci led to the description of on the basis of their action on gelatin and on many new staphylococcal species.
    [Show full text]