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Pierce's Disease Research Symposium Proceedings for 2008

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Pierce's Disease Research Symposium Proceedings for 2008 Proceedings of the 2008 Pierce’s Disease Research Symposium December 15-17, 2008 Westin Gaslamp Quarter Hotel San Diego, California Organized by: California Department of Food and Agriculture Chief Editor: Thomas Esser Compiling, Formatting, Proofreading, and Editing: Peggy Blincoe, Doug West, Sean Veling, Raj Randhawa, and Janet LeMasters Cover Photograph and Design: Sean Veling Printer: Copeland Printing, Sacramento, California Funds for Printing Provided By: CDFA Pierce’s Disease and Glassy-winged Sharpshooter Board To Order Additional Copies of this Publication, Please Contact: Pierce’s Disease Control Program California Department of Food and Agriculture 1220 N Street, Room 325 Sacramento, California 95814 Telephone: (916) 651-0253 Fax: (916) 651-0275 http://www.cdfa.ca.gov/pdcp E-mail: [email protected] Cite as: Proceedings, 2008 Pierce’s Disease Research Symposium. California Department of Food and Agriculture, Sacramento, CA. Revision History: -- Nov. 24, 2008: pages 153, 154, 155, 160, 163 Disclaimer: The reports in this publication have not been subject to scientific peer review. The California Department of Food and Agriculture (CDFA) makes no warranty, expressed or implied, and assumes no legal liability for the information in this publication. The publication of the Proceedings by CDFA does not constitute a recommendation or endorsement of products mentioned. TABLE OF CONTENTS Section 1: Vector Biology and Ecology Immunohistochemistry of ß-1,4-Glucanase, the Major Enzymatic Component of Glassy-winged Sharpshooter Saliva, in Probed Grape Petioles Elaine A. Backus, John M. Labavitch, Kim Kingston, Holly Shugart, L. Carl Greve, and David Morgan................................. 3 Detection and Analysis of Xylella fastidiosa in Glassy-winged Sharpshooter Populations in Texas Blake Bextine and Daymon Hail ................................................................................................................................................. 7 Using an Orange Oil Solvent to Remove Insects from Yellow Sticky Traps: Impacts on the Detection of Xylella fastidiosa DNA in Glassy-winged Sharpshooter Samples Blake Bextine and Patrick Marshall .......................................................................................................................................... 12 Comparative Genomics: Identifying Similarities and Differences Across Three Leafhopper Vectors of Xylella fastidiosa Wayne B. Hunter ....................................................................................................................................................................... 16 Assessing the Post-Winter Threat of Glassy-winged Sharpshooter Populations Marshall W. Johnson, Kris Lynn-Patterson, Mark Sisterson, and Russell Groves .................................................................... 22 Reproductive Behavior of a Key Vector of Xylella fastidiosa in Texas Isabelle Lauzière........................................................................................................................................................................ 28 Investigation into the Incidence and Distribution of Pierce’s Disease and Its Vectors in a Previously Considered “Very Low Risk” Texas Winegrape Growing Region Isabelle Lauzière and Jacy L. Lewis.......................................................................................................................................... 30 Cold Storage of Gonatocerus ashmeadi Girault: Extended Emergence, and Parental and Progeny Fitness Roger A. Leopold ...................................................................................................................................................................... 35 Faunistic Analysis of Sharpshooters in Plum Orchards of Rio Grande Do Sul State, Brazil Cristiane Müller, Wilson Azevedo Filho, Marcos Bottom, and João R.S. Lopes...................................................................... 39 Seasonal Transmission of Xylella fastidiosa by the Glassy-winged Sharpshooter from Grapevines Infected for Various Lengths of Time Thomas M. Perring .................................................................................................................................................................... 43 Identifying the Species of Mymaridae Reared in Argentina and Mexico for Potential Introduction to California Against the Glassy-winged Sharpshooter and Preparing and Submitting for Publication a Pictorial Annotated Key to the Ater-Group Species of Gonatocerus, Egg Parasitoids of the Proconiine Sharpshooters in the Neotropical Region Serguei V. Triapitsyn................................................................................................................................................................. 49 - i - Section 2: Vector Management Age Determination and the Red Pigment in the Wings of the Glassy-winged Sharpshooter Blake Bextine.............................................................................................................................................................................53 Understanding the Dynamics of Neonicotinoid Insecticidal Activity Against the Glassy-winged Sharpshooter: Development of Target Thresholds in Grapevines Frank J. Byrne and Nick C. Toscano .........................................................................................................................................55 RNA-Interference and Control of the Glassy-winged Sharpshooter and Other Leafhopper Vectors of Xylella fastidiosa Bryce W. Falk and Cristina Rosa...............................................................................................................................................57 Are Glassy-winged Sharpshooter Populations Regulated in California? Long-term Phenological Studies for Glassy-Winged Sharpshooter in an Organic Lemon Orchard Mark S. Hoddle..........................................................................................................................................................................63 Should Neoclassical Biological Control Agents from Argentina Be Released in California for Control of the Glassy-winged Sharpshooter? Mark Hoddle and Nic Irvin........................................................................................................................................................66 Improved Detection, Monitoring, and Management of the Glassy-winged Sharpshooter Russell F. Mizell, III and Peter C. Andersen .............................................................................................................................70 Riverside County Glassy-winged Sharpshooter Area-Wide Management Program in the Coachella and Temecula Valleys Nick C. Toscano and Carmen Gispert........................................................................................................................................74 Development of a Pest Management Program for the Control of Leafhopper Vectors of Xylella fastidiosa in North Carolina Vineyards Raul Villanueva, Turner Sutton, and George Kennedy..............................................................................................................77 Section 3: Pathogen Biology and Ecology Biology of the Xylella fastidiosa-Vector Interface Rodrigo Almeida and Nabil Killiny...........................................................................................................................................83 Evolution of Xylella fastidiosa Avirulence Rodrigo Almeida and Leonora Bittleston ..................................................................................................................................87 Genetic Diversity Analysis of Xylella fastidiosa Strains Using Multiple TONB Genes and the ZOT Gene Blake Bextine, Lisa Morano, Stanley Gunawan, and Henry Schreiber Jr..................................................................................90 The Significance of Xylella fastidiosa Type I and Type IV Pili in Biofilm Structure, Bacterial Survival in Biofilms, and DNA Secretion and Uptake Thomas J. Burr and Harvey C. Hoch .........................................................................................................................................93 Differential Expression of Genes of Xylella fastidiosa in Xylem Fluid of Citrus and Grapes Donald A. Cooksey....................................................................................................................................................................97 - ii - Role of Type I Secretion in Pierce’s Disease Dean W. Gabriel...................................................................................................................................................................... 101 Understanding Control of Xylella fastidiosa Cell Aggregation: Importance in Colonization and Biofilm Development in Grapevine and Sharpshooter Foregut Harvey C. Hoch, Thomas J. Burr, Leonardo De La Fuente, Paulo Zaini, and Luciana Cursino.............................................. 106 Exploiting a Chemosensory Signal Transduction System that Controls Twitching Motility and Virulence in Xylella fastidiosa Harvey C. Hoch, Thomas J. Burr, Patricia Mowery, Luciana Cursino, Paulo Zaini, and Leonardo De La Fuente ................. 110 The Role of Type V Secretion Autotransporters in the Virulence of Xylella fastidiosa Michele M. Igo .......................................................................................................................................................................
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