Effects of /I-Xylosides on Proteoglycan Biosynthesis and Morphology of PC12 Pheochromocytoma Cells and Primary Cultures of Rat Cerebellum
Effects of /i-xylosides on proteoglycan biosynthesis and morphology of PC12 pheochromocytoma cells and primary cultures of rat cerebellum R. K. MARGOLIS*, B. GOOSSEN, H. TEKOTTE, L. HILGENBERG and R. U. MARGOLIS Department of Pharmacology, State University of New York, Health Science Center, Brooklyn, New York 11203, and Department of Pharmacology, New York University Medical Center, New York, New York 10016, USA • Author for correspondence at: Department of Pharmacology, Box 29, State University of New York, Health Science Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA Summary We have examined the effects of /J-xylosides, which ively). /J-Xyloside inhibition of proteoglycan biosyn- act as exogenous acceptors for glycosaminoglycan thesis was accompanied by significant morphologi- chain initiation, on the morphology and proteogly- cal effects in NGF-treated PC12 cells, consisting of an can biosynthesis of PC 12 pheochromocytoma cells, increase in length and decrease in the branching, and on monolayer, aggregate and explant cultures of diameter and adhesion to the collagen substratum of early postnatal rat cerebellum. PC 12 cells cultured the PC 12 cell processes. p-Nitrophenyl- and for 13 days in the presence of nerve growth factor 4-methylumbeUiferyl-/S-D-xylosides produced similar (NGF) and /J-xyloside, and labeled during days 11-13 effects, which were not seen with p-nitrophenyl-/J-D- with sodium [3SS]sulfate, showed an 8- to 11-fold galactoside. /J-Xylosides also produced distinct alter- increase in [3BS]sulfate-labeled macromolecules re- ations in the adhesion and morphology of monolayer, leased into the culture medium. Most of the increase aggregate, and explant cultures of early postnatal rat was accounted for by chondroitin sulfate, which was cerebellum, which occurred together with inhibition in the form of free glycosaminoglycan chains, which of chondroitin sulfate proteoglycan biosynthesis and were not acid-precipitable.
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