Aquatic Microbial Ecology 61:73

Home , Methylophaga thalassica, Piscirickettsiaceae

The following supplement accompanies the article

Bacterial diversity of Gymnodinium catenatum and its relationship to dinoflagellate toxicity

David H. Green1,*, Mark C. Hart1, Susan I. Blackburn2, Christopher J. S. Bolch3

1Scottish Association for Marine Science, Dunstaffnage Marine Laboratory, Oban PA37 1QA, UK 2CSIRO Marine and Atmospheric Research, Hobart, Tasmania 7000, Australia 3National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia

*Email: [email protected]

Aquatic Microbial Ecology 61: 73–87 (2010)

Supplementary Table S1. Phylogenetic affiliation of Gymnodinium catenatum operational taxonomic units (OTUs)a OTU G. catenatum OTU Accession Accession ID No. Abundance Closest relative in the RDP II No. (%) Taxonomic Affiliation (%)b Alphaproteobacteria; Caulobacterales; Hyphomonadaceae GCDE08_V AY701470 3.2 Oceanicaulis alexandrii C116-18 AJ309862 93.8 GCJP01_C AY701442 10.6 Hyphomonas oceanitis SCH-89 AF082797 95.4 YC499B15_A AY701430 1.1 Maricaulis maris ATCC 15268 AJ227802 96.2 YC499B15_AE AY701434 1.1 Hyphomonas oceanitis SCH-89 AF082797 95.5 Alphaproteobacteria; Rhizobiales; Phyllobacteriaceae GCTRA14_W AY701464 2.3 Ahrensia sp. DFL-11 AJ582083 99.0 YC499B15_J AY701437 3.2 Mesorhizobium amorphae ATCC 19665 AF041442 90.2 Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae CAWD101_H AY701427 7.9 Alphaproteobacterium ML6 AJ315682 95.8 GC21V_T AY701424 1.1 Roseobacter sp. LA7 AF513438 95.1 GCDE08_W AY701471 8.5 Stappia aggregata IAM12614 D88520 96.1 GCDE08_Z AY701472 1.1 Roseobacter sp. ISM AF098495 89.7 GCHU11_A AY701453 2.1 Rhodobacter apigmentans AF03543 94.6 GCHU11_G AY701455 15.8 Roseobacter sp. KT0202a AF305498 96.5 GCJP01_V AY701449 2.1 Ruegeria gelatinovorans IAM12617 D88523 93.5 GCTRA14_O AY701459 9.3 Roseobacter sp. DSS-8 AF098493 94.9 YC499B15_W AY701440 2.1 Roseobacter litoralis ATCC 49566 X78312 95.4 Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae GC21V_AC AY701418 2.1 Bacterium L12 AY345389 94.9 YC499B15_M AY701438 3.2 Thalassospira lucentensis AF358664 91.8 YC499B15_AD AY701433 1.1 Bacterium L12 AY345389 95.3 Alphaproteobacteria; Rhodospirillales; Unclassified GCJP01_M AY701445 2.1 Uncultured bacterium ZA3420c AF382120 88.4 Uncultured alpha proteobacterium GCJP01_X AY701451 2.1 AF353236 88.1 Arctic97A-7 Uncultured alpha proteobacterium GCTRA14_R AY701460 4.7 AF353236 89.4 Arctic97A-7 Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae AB06210 CAWD101_AB AY701426 1.1 Porphyrobacter sanguineus ATCC 25661 96.8 6 Gammaproteobacteria; Alteromonadales; Alteromonadaceae CAWD101_Q AY701428 5.6 Marinobacter hydrocarbonoclasticus ATCC AB019148 95.9 27132 GCJP01_P AY701446 6.4 Alteromonas macleodii KT1113 AF173965 89.6

1 Gammaproteobacteria; Gammaproteobacteria_incertae_sedis YC499B15_AB AY701432 1.1 Microbulbifer sp. CJ11049 AF500206 83.2 Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae GC21V_D AY701422 4.3 Unidentified gamma proteobacterium DG940 AY258111 92.8 Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae GC21V_AI AY701420 1.0 Methylophaga thalassica DSM5690 X95460 94.0 Gammaproteobacteria; Unclassified GC21V_AD AY701419 1.0 Marine gamma proteobacterium HTCC2188 AY386344 95.9 GCDE08_AB AY701465 1.1 Marine gamma proteobacterium HTCC2188 AY386344 89.8 Uncultured gamma proteobacterium E01-9C- GCDE08_AE AY701467 2.1 12 AJ581349 93.1 GCHU11_C AY701454 5.3 Uncultured gamma-Proteobacterium OM241 U70702 93.3 Uncultured gamma proteobacterium GCJP01_R AY701448 17.0 AEGEAN133 AF406527 92.8 YC499B15_O AY701439 8.4 Marine gamma proteobacterium HTCC2188 AY386344 97.2 Bacteroidetes; Flavobacteria; Flavobacteriales; Cryomorphaceae GC21V_AB AY701417 4.3 Owenweeksia hongkongensis UST20020801 AB125062 84.5 Bacteroidetes; Flavobacteria; Flavobacteriales; Flavobacteriaceae GCDE08_K AY701469 2.1 Winogradskyella poriferorum UST030701-29 AY848823 96.7 GCTRA14_K AY701458 2.3 Cytophaga fucicola NN015860 AJ005973 91.7 Bacteroidetes; Sphingobacteria; Sphingobacteriales; Flammeovirgaceae CAWD101_R AY701429 1.1 Reichenbachia agariperforans KMM 3525 AB058919 91.8 GCDE08_AD AY701466 2.1 Microscilla furvescens IFO 15994 AB078079 85.8 GCJP01_D AY701443 6.4 Microscilla furvescens IFO 15994 AB078079 85.6 GCJP01_Y AY701452 2.1 Bacteroidetes bacterium GMD13F04 AY162116 91.0 GCTRA14_A AY701456 2.3 Reichenbachia agariperforans KMM 3525 AB058919 90.0 GCTRA14_S AY701461 4.7 Microscilla furvescens IFO 15994 AB078079 91.2 GCTRA14_T AY701462 4.7 Flexibacter aggregans IFO 15974 AB078038 90.9 YC499B15_D AY701435 3.2 Flexibacter aggregans IFO 15974 AB078038 90.5 YC499B15_AA AY701431 2.1 Reichenbachia agariperforans KMM 3525 AB058919 89.5 Bacteroidetes; Sphingobacteria; Sphingobacteriales; Chitinophagaceae GC21V_H AY701423 13.0 Bacteroidetes bacterium GMDJE10E6 AY162091 91.7 GC21V_U AY701425 9.8 Bacteroidetes bacterium GMDJE10E6 AY162091 91.6 GCDE08_C AY701468 4.3 Bacteroidetes bacterium GMDsbC7 AY162093 88.9 GCJP01_A AY701441 8.5 Bacteroidetes bacterium GMDJE10E6 AY162091 97.0 GCJP01_Q AY701447 6.4 Bacteroidetes bacterium GMDJE10E6 AY162091 92.2 GCTRA14_I AY701457 20.9 Bacteroidetes bacterium GMDJE10E6 AY162091 96.5 YC499B15_H AY701436 22.1 Bacteroidetes bacterium GMDJE10E6 AY162091 97.7 Bacteroidetes; Bacteroidetes_incertae_sedis; Fulvivirga GC21V_AJ AY701421 1.0 Flexibacter aggregans IFO 15974 AB078038 84.5 Planctomycetes; Planctomycetales; Planctomycetaceae GCJP01_I AY701444 8.5 Planctomyces brasiliensis DSM 5305 AJ231190 82.5 GCJP01_W AY701450 2.1 Uncultured planctomycete SIMO-1878 AY711244 93.9 Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae GC21V_A AY701416 26.1 Fucophilus fucoidanolyticus AB073978 97.1 Verrucomicrobia; Verrucomicrobiales; Verrucomicrobiaceae GCTRA14_U AY701463 4.7 Uncultured Verrucomicrobia PI-RT83 AY580845 94.9 aOTUs are designated based on the strain of G. catenatum and alphabetic code denoting the ARDRA pattern (e.g. AA). Taxonomic classification is based on the RDP II CLASSIFIER and the closest characterised relative on the RDP II (Wang et al. 2007). bRelative abundance of each OTU in its respective clone library.