DETERMINAZIONE DEL DIRETTORE DEL SERVIZIO N. 029/18 DEL 28 Marzo 2018 (C.D.R. 00.00.01.04) Oggetto: Progetto “Diversity of L

Servizio Ricerca per la Zootecnia

DETERMINAZIONE DEL DIRETTORE DEL SERVIZIO N. 029/18 DEL 28 Marzo 2018 (C.d.R. 00.00.01.04)

Progetto “Diversity of local pig breeds and production systems for high quality traditional Oggetto: products and sustainable pork chains – TREASURE” - Approvazione Convenzione con il Dipartimento di Medicina Veterinaria dell’Università degli studi di Sassari. WBS PA15RE03 - CUP B82I15001450006

VISTA la L.R. 13 novembre 1998, n. 31, avente ad oggetto “Disciplina del personale regionale e dell'organizzazione degli uffici della Regione” e successive modificazioni ed integrazioni;

VISTA la L.R. 8 agosto 2006, n. 13, avente ad oggetto “Riforma degli enti agricoli e riordino delle funzioni in agricoltura. Istituzione delle Agenzie AGRIS Sardegna, LAORE Sardegna e ARGEA Sardegna”;

VISTO lo Statuto dell’Agenzia per la Ricerca in Agricoltura AGRIS Sardegna approvato con deliberazione della Giunta regionale n. 25/37 del 3.07.2007 così come modificato con deliberazioni della Giunta regionale n. 58/13 del 28.10.2008 e n. 21/44 del 3.06.2010;

VISTA la L.R. 25 novembre 2014 n. 24, avente ad oggetto “Disposizioni urgenti in materia di organizzazione della Regione”;

VISTO il decreto del Presidente della Regione n. 16 dell’8.04.2016 avente ad oggetto “Legge Regionale 8 agosto 2006, n. 13, art. 30. Agenzia per la Ricerca in Agricoltura della Regione Autonoma della Sardegna (AGRIS Sardegna). Nomina Direttore Generale, con il quale sono state conferite al Dott. Roberto Zurru le funzioni di Direttore Generale dell’Agenzia;

VISTA la L.R. 13 aprile 2017, n. 5, avente ad oggetto “legge di stabilità 2017”;

VISTA la determinazione del Direttore Generale n. 141/17 del 23.06.2017 avente ad oggetto “Approvazione del bilancio di previsione per gli anni 2017-2019”, reso esecutivo con delibera della Giunta Regionale n. 36/15 del 25.07.2017;

VISTA la determinazione del Direttore Generale n. 274/17 del 21.12.2017 avente ad oggetto: “Autorizzazione all’esercizio provvisorio del bilancio dell’Agenzia Agris per l’anno 2018” per mesi quattro dall’1 gennaio al 30 aprile 2018, come da delibera di G.R. n. 3/27 del 23.01.2018;

VISTA la determinazione del Direttore Generale n. 181/15 del 24.06.2015 con cui al dott. Sandro Delogu è stato conferito l’incarico di posizione dirigenziale del Servizio ricerca per la zootecnia a far data dal 01.07.2015;

PREMESSO che  con il Grant Agreement for Research and Innovaction n. 634476 – TREASURE – H2020-SFS-2014-2015/H2020- SFS-2014-2 tra l’Agenzia REA della Commissione Europea e, tra gli altri, l’Agris Sardegna venivano concordati e definiti gli adempimenti relativi al corretto svolgimento delle attività previste nel progetto di ricerca “Diversity of local pig breeds and production systems for high quality traditional products and sustainable pork chains”del quale Agris è individuata come beneficiario;  l’Agris ha individuato il Dipartimento di Medicina Veterinaria dell’Università di Sassari – Ispezione degli Alimenti di Origine Animale – come “parte terza” per lo svolgimento delle attività previste nel WP5 Task 5,4 del programma di ricerca;

CONSIDERATO che:  occorre definire nel dettaglio i rapporti finanziari ed operativi tra i due soggetti;

AGRIS Sardigna- Agentzia pro sa chirca in agricultura AGRIS Sardegna - Agenzia per la ricerca in agricoltura Sassari, località Bonassai SS 291 km 18,6 – P.IVA. 02270290907 – tel. +39 079 2842300 - fax +39 079 389450

Servizio Ricerca per la Zootecnia DETERMINAZIONE N. 029/18 DEL 28 Marzo 2018

 a tal fine gli uffici hanno predisposto apposita bozza di convenzione e la stessa è già stata condivisa per le vie brevi dal partner scientifico;  è necessario approvare la convenzione di cui sopra, regolante i rapporti reciproci e le modalità di trasferimento delle somme assegnate, allegata alla presente determinazione per farne parte integrante e sostanziale;  in applicazione delle norme in materia di armonizzazione contabile e riaccertamento residui, tutte le risorse a destinazione vincolata finanziate per la realizzazione dei diversi progetti di ricerca, incluso quello di cui all’oggetto, potranno essere impegnate e utilizzate nell’esercizio finanziario corrente solo previa approvazione del conto Preconsuntivo e successiva variazione per la re imputazione alla competenza 2018;  in attesa del perfezionamento dell’iter burocratico per l’imputazione della spesa, si debba comunque procedere all’avvio di tutte le procedure utili al fine di garantire il regolare svolgimento dell’attività di ricerca e non determinare pregiudizio ai rigidi cronoprogrammi approvati dal soggetto finanziatore

D E T E R M I N A  di approvare, per i motivi espressi in narrativa, la convenzione tra l’Agenzia Agris Sardegna e il Dipartimento di Medicina Veterinaria dell’Università degli studi di Sassari , che si allega come parte integrante e sostanziale del presente provvedimento;  di impegnare, per i motivi di cui in narrativa, la cifra complessiva di € 6.000,00 sul bilancio 2018 disponendo sin d’ora che, ad avvenuta firma della Convenzione, il Servizio Bilancio disponga per la liquidazione e pagamento dell’importo totale entro 30 gg dalla sottoscrizione della allegata presente convenzione sul Conto di Tesoreria Unica Bankitalia, IBAN: IT26G0100003245522300034835, Causale: Progetto Treasure.  di impegnare, per i motivi di cui in narrativa, la cifra complessiva di € 6.000.00 sul bilancio 2018 a titolo di corrispettivo per le attività svolte nell’ambito del progetto disponendo sin d’ora che, ad avvenuta firma della Convenzione, il Servizio Bilancio disponga per la liquidazione e pagamento dell’importo totale sul Conto di Tesoreria Unica Banca Italia, IBAN: IT26G0100003245522300034835, Causale Progetto Treasure;  di provvedere con successivo atto al formale impegno della somma complessiva di € 6.000,00 sul competente capitolo di bilancio per l’esercizio 2018;  di assumere l’incarico di Responsabile Unico del Procedimento ai sensi dell’art. 31 del D.Lgs. n. 50/2016;  di nominare in qualità di direttore dell’esecuzione del presente contratto il dott. Marco Acciaro;  di pubblicare la presente determinazione sul sito intranet dell’Agris Sardegna e di trasmetterne copia digitale al Servizio Bilancio e Contabilità per gli adempimenti conseguenti e al Direttore generale per conoscenza.

Il Direttore del Servizio Dr. Sandro S. Delogu

2/2

CONVENZIONE PER L’ATTUAZIONE DEGLI INTERVENTI NELL’AMBITO DELLA RICERCA PER IL PROGETTO “DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS – TREASURE” (CUP B82I15001450006)

as the Beneficiary of the H2020 project TREASURE – Grant agreement no. 634476 and linked to Third Party (TP) UNISS

TRA

AGRIS SARDEGNA, come Beneficiary of the H2020 project TREASURE – Grant agreement no. 634476, codice fiscale 02270290907, Servizio Ricerca per la Zootecnia, nella persona del Direttore del Servizio Ricerca per la Zootecnia, Sandro Delogu, domiciliato per la carica presso la sede di Agris Sardegna, Località Bonassai SS 291 km 18,6, 07100 Sassari (SS);

DIPARTIMENTO DI MEDICINA VETERINARIA DELL’UNIVERSITÀ DEGLI STUDI DI SASSARI, come linked Third Party Grant Agreement N° 634476, CF e P.IVA 00196350904, nella persona del Direttore Prof. Eraldo Sanna Passino domiciliato per la carica presso la sede del Dipartimento, via Vienna n. 2/a, 07100 Sassari (SS);

PREMESSE

. Visto il Grant Agreement for Research and Innovation action N° 634476 — TREASURE — H2020-SFS- 2014-2015/H2020-SFS-2014-2 (Traditional resources for agricultural diversity and the food chain) tra l’Agenzia REA (Research Executive Agency) della Commissione Europea e, tra gli altri, Agris Sardegna nella quale vengono concordati e definiti gli adempimenti relativi al corretto svolgimento delle attività inerenti l’esecuzione del progetto di ricerca: DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS — TREASURE e per il quale Agris è individuata come beneficiario;

. visto che Agris ha individuato a sua volta l’Università degli Studi Di Sassari e precisamente il Dipartimento di Medicina Veterinaria – Ispezione degli Alimenti di Origine Animale come “linked third party” (parte terza, Grant Agreement N° 634476 Art. 14.1 pag. 28 e Consortium Agreement paragrafo 9.5 pag. 18 e suo Attachment 4) per svolgere le attività previste nel WP5 Task 5.4 del succitato progetto TREASURE, così come specificato nel Grant Agreement N° 634476;

 visto il ruolo di responsabile previsto per Agris dal progetto TREASURE, anche nei riguardi della sua parte terza UNISS si è ritenuto di regolare i rapporti finanziari e operativi con la presente convenzione;

CONVENGONO E STIPULANO quanto segue, con le premesse facenti parte integrante della Convenzione

Art. 1 – Obiettivi e finalità Le parti si impegnano a realizzare il progetto di ricerca “DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS - TREASURE” secondo le modalità e i contenuti approvati nel progetto stesso (Allegato Annex 1 Description of action part A), di cui AGRIS SARDEGNA è beneficiario e il DIPARTIMENTO DI MEDICINA 1

VETERINARIA come parte terza, secondo le modalità del Grant Agreement for Research and Innovation action N° 634476 e delle relative Linee guida, allegati entrambi alla presente convenzione per farne parte integrante e sostanziale. Ciascuna parte eseguirà le prestazioni di propria competenza in totale autonomia fiscale, gestionale e operativa, con personale responsabilità in ordine alla perfetta esecuzione dei compiti a ciascuno affidati. In particolare, con la presente Convenzione:  AGRIS si impegna a svolgere le funzioni di coordinamento, del monitoraggio e della rendicontazione della parte relativa ad AGRIS del progetto di ricerca “DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS – TREASURE” così come stabilito in Annex 1 part A, task 5.4 pag. 50

 Il DIPARTIMENTO DI MEDICINA VETERINARIA si impegna a: 1) svolgere le attività previste per quanto di sua competenza come stabilito in Annex 1 part B, pag 80 2) provvedere alla corretta rendicontazione dei costi sostenuti in relazione al progetto di ricerca; 3) trasmettere ad Agris Sardegna in modalità informatica e in copia conforme, la documentazione relativa alla rendicontazione finale in tempo utile rispetto a quanto previsto nel documento Grant Agreement N° 634476 Art. 18.1.2 pag. 32 allegato alla presente e nelle tempistiche indicate da AGRIS Sardegna.

Le parti si impegnano, inoltre, a fornire il più ampio quadro di collaborazione per la realizzazione dell’intervento.

Art. 2 – Responsabili scientifici delle attività AGRIS SARDEGNA individua come Responsabile scientifico del Progetto il Dott. Marco Acciaro, il DIPARTIMENTO DI MEDICINA VETERINARIA individua il Prof. Enrico Pietro Luigi De Santis.

Art. 3 – Finanziamento del progetto Agris Sardegna trasferirà al DIPARTIMENTO DI MEDICINA VETERINARIA, in un’unica soluzione anticipata l’importo di propria competenza ovvero: - € 6.000,00 (euro seimila/00) sul Conto di Tesoreria Unica Bankitalia, IBAN: IT26G0100003245522300034835, Causale: Progetto Treasure Alla scadenza del Progetto, il Dipartimento di Medicina veterinaria trasmetterà ad Agris Sardegna, in modalità informatica, la relazione scientifica e la rendicontazione finale, per la quota di propria competenza.

Art. 5 – Obblighi di pubblicità. Tutte le pubblicazioni e gli eventi realizzati nell’ambito dei progetti di cui alla presente Convenzione dovranno citare espressamente “This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 634476” e mostrare l’emblema dell’EU

Art. 6 – Obblighi di informazione. Il DIPARTIMENTO DI MEDICINA VETERINARIA si impegna a tenere informata AGRIS SARDEGNA di tutte le eventuali modifiche inerenti la conduzione del progetto.

Art. 7 – Obblighi di custodia. Sarà cura di AGRIS SARDEGNA conservare tutta la documentazione originale relativa al Progetto o copia conforme all’originale per quanto riguarda il DIPARTIMENTO DI MEDICINA VETERINARIA, per almeno 5 anni, in modo da essere disponibile per eventuali controlli.

Art. 8 – Riservatezza Tutta la documentazione e le informazioni di carattere tecnico e metodologico, fornite da una parte all’altra, dovranno essere considerate di carattere confidenziale e non potranno essere utilizzate per scopi diversi da quelli per i quali sono state fornite, senza previa autorizzazione della parte che le ha fornite. 2

Esse non potranno essere utilizzate, per scopi diversi da quelli per i quali sono state fornite, senza una preventiva autorizzazione scritta dal soggetto che le ha fornite. Ciascuno dei soggetti avrà cura di applicare le opportune misure per mantenere circoscritte le informazioni e le documentazioni ottenute.

Art. 9 – Controversie e foro competente Per la risoluzione di ogni eventuale controversia, relativamente all’attuazione del presente accordo, che non si possa definire in via amichevole, le parti stabiliscono di eleggere esclusivamente quale foro competente il Tribunale di Sassari.

Allegati: A) Grant agreement No 634476 B) Annex 1 Description Of Action part A Research and Innovation action NUMBER — 634476 — TREASURE C) Annex 1 Description Of Action part B Research and Innovation action NUMBER — 634476 — TREASURE D) TREASURE 634476 - Consortium Agreement

Sassari, ______

Direttore del Servizio Ricerca per la Zootecnia Dott. Sandro Delogu

Direttore del Dipartimento di Medicina Veterinaria dell’Università di Sassari Prof. Eraldo Sanna Passino

Il Direttore del Servizio Dr. Sandro Delogu

Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

SFS-07a-2014: Traditional resources for agricultural diversity and the food chain

Grant agreement for: Research and Innovation action

Grant Agreement

Action acronym: TREASURE Action full title: "DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS " Grant agreement no: 634476

1 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

EUROPEAN COMMISSION Research Executive Agency (REA)

Director

GRANT AGREEMENT

NUMBER — 634476 — TREASURE

This Agreement (‘the Agreement’) is between the following parties: on the one part, the Research Executive Agency (REA) ('the Agency'), under the power delegated by the European Commission ('the Commission')1, represented for the purposes of signature of this Agreement by Head of Unit, Research Executive Agency (REA), Industrial Leadership and Societal Challenges Department, Sustainable Resources for Food Security and Growth, Kerstin ROSENOW, and on the other part, 1. ‘the coordinator’: KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS), 5055431, established in Hacquetova ulica 17, LJUBLJANA 1000, Slovenia, SI23887729, represented for the purposes of signing the Agreement by director, Andrej SIMONČIČ and the following other beneficiaries, if they sign their ‘Accession Form’ (see Annex 3 and Article 56): 2. UNIVERZA V LJUBLJANI (UL), 5085063, established in KONGRESNI TRG 12, LJUBLJANA 1000, Slovenia, SI54162513, 3. KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM) SI2, 1625403000, established in SMIHELSKA CESTA 14, NOVO MESTO 8000, Slovenia, SI70711798, 4. IFIP-INSTITUT DU PORC ASSOCIATION (IFIP) FR3, 775681323/00051317P, established in RUE LESPAGNOL 5, PARIS 75020, France, FR13775681323, 5. INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA), 180070039, established in Rue De L'Universite 147, PARIS CEDEX 07 75338, France, FR57180070039, 6. BAUERLICHE ERZEUGERGEMEINSCHAFT SCHWABISCH HALL WV (BESH) WV, 1511131/CF8406202485, established in HALLER STRASSE 20, WOLPERTSHAUSEN 7179, Germany, DE146787977, 7. SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG), 080159438, established in SVETOSIMUNSKA CESTA 25, ZAGREB 10000, Croatia, HR76023745044, 8. SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFOS) HR6, 030036907, established in KRALJA PETRA SVACICA 1/ D, OSIJEK 31000, Croatia, HR98816779821,

1 Text in italics shows the options of the Model Grant Agreement that are applicable to this Agreement.

2 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

9. AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA (AGRIS), CF02270290907, established in LOCALITA BONASSAI SS 291 KM 18 6, SASSARI 07100, Italy, IT02270290907, 10. ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI (ANAS) IT2, 862427CF0272290584, established in VIA LAZZARO SPALLANZANI 4, ROMA 00161, Italy, IT01028581005, 11. UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI), R.D. n. 2102/1923, established in Piazza San Marco 4, Florence 50121, Italy, IT01279680480, 12. CENTRI REGIONALI PER LE TECNOLOGIEAGROALIMENTARI SCARL (CERTA) SCARL, 251120CF03496570718, established in VIA GRAMSCI 89/91, FOGGIA 71100, Italy, IT03496570718, 13. ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA (UNIBO), CF80007010376, established in VIA ZAMBONI 33, BOLOGNA 40126, Italy, IT01131710376, 14. STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA) IT14, 217562/CF00166540344, established in VIALE FAUSTINO TANARA 31/A, PARMA 43100, Italy, IT00166540344, 15. LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS), 302536989 , established in A MICKEVICIAUS GATVE 9, KAUNAS 44307, Lithuania, LT100005579315 , 16. INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC) PCUP, 503791877, established in Praça General Barbosa, Viana do Castelo 4900, Portugal, PT503761877, 17. UNIVERSIDADE DE EVORA (UEVORA) PCUP, 482/79, established in Largo dos Colegiais 2, Evora 7000-803, Portugal, PT501201920, 18. INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN (IAH), 07017537/538200, established in AUTOPUT 16, BEOGRAD 11080, Serbia, RS100014262, 19. FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG), 07029845, established in Nemanjina 6, BELGRADE 11080, Serbia, CS100198802, 20. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA), na, established in Carretera de la Coruna Km7.5, MADRID 28040, Spain, ESQ2821013F, 21. AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) ES8, established in CALLE SERRANO 117, MADRID 28006, Spain, ESQ2818002D, 22. CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC-IRTA (CREDA) ES3, 2214, established in PARC MEDITERRANI DE LA TECNOLOGIA EDIFICI ESAB C/ESTEVE TERRRADES 8, CASTELLDEFELS 08860, Spain, ESG64068463, 23. CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX), established in AUTOVIA A5 KM 372 FINCA LA ORDEN GUADAJIRA, LOBON BADAJOZ 06187, Spain, ESS0600428G, 24. INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA), established in CTRA C-59 KM 12 TORRE MARIMON, CALDES DE MONTBUI BARCELONA 08140, Spain, ESQ5855049B, 25. ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO (AECERIBER) ES5, 3474/603368, established in CALLE SAN FRANCISCO 51 1 DERECHA, ZAFRA BADAJOZ 06300, Spain, ESG06053334,

Unless otherwise specified, references to ‘beneficiary’ or ‘beneficiaries’ include the coordinator.

3 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The parties referred to above have agreed to enter into the Agreement under the terms and conditions below.

By signing the Agreement or the Accession Form, the beneficiaries accept the grant and agree to implement it under their own responsibility and in accordance with the Agreement, with all the obligations and conditions it sets out.

The Agreement is composed of:

Terms and Conditions

Annex 1 Description of the action

Annex 2 Estimated budget for the action

Annex 3 Accession Forms

Annex 4 Model for the financial statements

Annex 5 Model for the certificate on the financial statements

Annex 6 Model for the certificate on the methodology

4 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

TERMS AND CONDITIONS

TABLE OF CONTENTS

CHAPTER 1 GENERAL...... 13

ARTICLE 1 — SUBJECT OF THE AGREEMENT...... 13

CHAPTER 2 ACTION...... 13

ARTICLE 2 — ACTION TO BE IMPLEMENTED...... 13

ARTICLE 3 — DURATION AND STARTING DATE OF THE ACTION...... 13

ARTICLE 4 — ESTIMATED BUDGET AND BUDGET TRANSFERS...... 13

4.1 Estimated budget...... 13

4.2 Budget transfers...... 13

CHAPTER 3 GRANT...... 13

ARTICLE 5 — GRANT AMOUNT, FORM OF GRANT, REIMBURSEMENT RATES AND FORMS OF COSTS...... 13

5.1 Maximum grant amount...... 13

5.2 Form of grant, reimbursement rates and forms of costs...... 14

5.3 Final grant amount — Calculation...... 14

5.4 Revised final grant amount — Calculation...... 16

ARTICLE 6 — ELIGIBLE AND INELIGIBLE COSTS...... 16

6.1 General conditions for costs to be eligible...... 16

6.2 Specific conditions for direct costs to be eligible...... 17

6.3 Conditions for costs of linked third parties to be eligible...... 23

6.4 Conditions for in-kind contributions provided by third parties free of charge to be eligible...... 23

6.5 Ineligible costs...... 23

6.6 Consequences of declaration of ineligible costs...... 23

CHAPTER 4 RIGHTS AND OBLIGATIONS OF THE PARTIES...... 23

SECTION 1 RIGHTS AND OBLIGATIONS RELATED TO IMPLEMENTING THE ACTION...... 24

ARTICLE 7 — GENERAL OBLIGATION TO PROPERLY IMPLEMENT THE ACTION...... 24

7.1 General obligation to properly implement the action...... 24

7.2 Consequences of non-compliance...... 24

ARTICLE 8 — RESOURCES TO IMPLEMENT THE ACTION...... 24

ARTICLE 9 — IMPLEMENTATION OF ACTION TASKS BY BENEFICIARIES NOT RECEIVING EU FUNDING...... 24

9.1 Rules for the implementation of action tasks by beneficiaries not receiving EU funding...... 24

5 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

9.2 Consequences of non-compliance...... 24

ARTICLE 10 — PURCHASE OF GOODS, WORKS OR SERVICES...... 25

10.1 Rules for purchasing goods, works or services...... 25

10.2 Consequences of non-compliance...... 25

ARTICLE 11 — USE OF IN-KIND CONTRIBUTIONS PROVIDED BY THIRD PARTIES AGAINST PAYMENT...... 25

11.1 Rules for the use of in-kind contributions against payment...... 25

11.2 Consequences of non-compliance...... 26

ARTICLE 12 — USE OF IN-KIND CONTRIBUTIONS PROVIDED BY THIRD PARTIES FREE OF CHARGE...... 26

12.1 Rules for the use of in-kind contributions free of charge...... 26

12.2 Consequences of non-compliance...... 26

ARTICLE 13 — IMPLEMENTATION OF ACTION TASKS BY SUBCONTRACTORS...... 26

13.1 Rules for subcontracting action tasks...... 26

13.2 Consequences of non-compliance...... 27

ARTICLE 14 — IMPLEMENTATION OF ACTION TASKS BY LINKED THIRD PARTIES...... 28

14.1 Rules for calling upon linked third parties to implement part of the action...... 28

14.2 Consequences of non-compliance...... 29

ARTICLE 15 — FINANCIAL SUPPORT TO THIRD PARTIES...... 29

15.1 Rules for providing financial support to third parties...... 29

15.2 Financial support in the form of prizes...... 29

15.3 Consequences of non-compliance...... 29

ARTICLE 16 — PROVISION OF TRANS-NATIONAL OR VIRTUAL ACCESS TO RESEARCH INFRASTRUCTURE...... 29

16.1 Rules for providing trans-national access to research infrastructure...... 29

16.2 Rules for providing virtual access to research infrastructure...... 29

16.3 Consequences of non-compliance...... 29

SECTION 2 RIGHTS AND OBLIGATIONS RELATED TO THE GRANT ADMINISTRATION...... 30

ARTICLE 17 – GENERAL OBLIGATION TO INFORM...... 30

17.1 Obligation to provide information upon request...... 30

17.2 Obligation to keep information up to date and to inform about events and circumstances likely to affect the Agreement...... 30

17.3 Consequences of non-compliance...... 30

ARTICLE 18 — KEEPING RECORDS — SUPPORTING DOCUMENTATION...... 30

18.1 Obligation to keep records and other supporting documentation...... 30

18.2 Consequences of non-compliance...... 32

6 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

ARTICLE 19 — SUBMISSION OF DELIVERABLES...... 32

19.1 Obligation to submit deliverables...... 32

19.2 Consequences of non-compliance...... 32

ARTICLE 20 — REPORTING — PAYMENT REQUESTS...... 32

20.1 General obligation to submit reports...... 32

20.2 Reporting periods...... 32

20.3 Periodic reports — Requests for interim payments...... 33

20.4 Final report — Request for payment of the balance...... 34

20.5 Information on cumulative expenditure incurred...... 34

20.6 Currency for financial statements and conversion into euro...... 35

20.7 Language of reports...... 35

20.8 Consequences of non-compliance — Suspension of the payment deadline — Termination...... 35

ARTICLE 21 — PAYMENTS AND PAYMENT ARRANGEMENTS...... 35

21.1 Payments to be made...... 35

21.2 Pre-financing payment — Amount — Amount retained for the Guarantee Fund...... 35

21.3 Interim payments — Amount — Calculation...... 36

21.4 Payment of the balance — Amount — Calculation — Release of the amount retained for the Guarantee Fund...... 36

21.5 Notification of amounts due...... 37

21.6 Currency for payments...... 37

21.7 Payments to the coordinator — Distribution to the beneficiaries...... 37

21.8 Bank account for payments...... 38

21.9 Costs of payment transfers...... 38

21.10 Date of payment...... 38

21.11 Consequences of non-compliance...... 38

ARTICLE 22 — CHECKS, REVIEWS, AUDITS AND INVESTIGATIONS — EXTENSION OF FINDINGS...... 39

22.1 Checks, reviews and audits by the Commission and the Agency...... 39

22.2 Investigations by the European Anti-Fraud Office (OLAF)...... 41

22.3 Checks and audits by the European Court of Auditors (ECA)...... 41

22.4 Checks, reviews, audits and investigations for international organisations...... 41

22.5 Consequences of findings in checks, reviews, audits and investigations —Extension of findings...... 41

22.6 Consequences of non-compliance...... 43

ARTICLE 23 — EVALUATION OF THE IMPACT OF THE ACTION...... 43

23.1 Right to evaluate the impact of the action...... 43

7 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

23.2 Consequences of non-compliance...... 44

SECTION 3 RIGHTS AND OBLIGATIONS RELATED TO BACKGROUND AND RESULTS...... 44

SUBSECTION 1 GENERAL...... 44

ARTICLE 23a — MANAGEMENT OF INTELLECTUAL PROPERTY...... 44

23a.1 Obligation to take measures to implement the Commission Recommendation on the management of intellectual property in knowledge transfer activities...... 44

23a.2 Consequences of non-compliance...... 44

SUBSECTION 2 RIGHTS AND OBLIGATIONS RELATED TO BACKGROUND...... 44

ARTICLE 24 — AGREEMENT ON BACKGROUND...... 44

ARTICLE 25 — ACCESS RIGHTS TO BACKGROUND...... 45

25.1 Exercise of access rights — Waiving of access rights — No sub-licensing...... 45

25.2 Access rights for other beneficiaries, for implementing their own tasks under the action...... 45

25.3 Access rights for other beneficiaries, for exploiting their own results...... 45

25.4 Access rights for affiliated entities...... 45

25.5 Access rights for third parties...... 46

25.6 Consequences of non-compliance...... 46

SUBSECTION 3 RIGHTS AND OBLIGATIONS RELATED TO RESULTS...... 46

ARTICLE 26 — OWNERSHIP OF RESULTS...... 46

26.1 Ownership by the beneficiary that generates the results...... 46

26.2 Joint ownership by several beneficiaries...... 46

26.3 Rights of third parties (including personnel)...... 47

26.4 Agency ownership, to protect results...... 47

26.5 Consequences of non-compliance...... 48

ARTICLE 27 — PROTECTION OF RESULTS — VISIBILITY OF EU FUNDING...... 48

27.1 General obligation to protect the results...... 48

27.2 Agency ownership, to protect the results...... 48

27.3 Information on EU funding...... 48

27.4 Consequences of non-compliance...... 49

ARTICLE 28 — EXPLOITATION OF RESULTS...... 49

28.1 General obligation to exploit the results...... 49

28.2 Results that could contribute to European or international standards — Information on EU funding...... 49

28.3 Consequences of non-compliance...... 49

ARTICLE 29 — DISSEMINATION OF RESULTS — OPEN ACCESS — VISIBILITY OF EU FUNDING...... 49

29.1 General obligation to disseminate results...... 49

8 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

29.2 Open access to scientific publications...... 50

29.3 Open access to research data...... 51

29.4 Information on EU funding — Obligation and right to use the EU emblem...... 51

29.5 Disclaimer excluding Agency responsibility...... 51

29.6 Consequences of non-compliance...... 51

ARTICLE 30 — TRANSFER AND LICENSING OF RESULTS...... 51

30.1 Transfer of ownership...... 51

30.2 Granting licenses...... 52

30.3 Agency right to object to transfers or licensing...... 52

30.4 Consequences of non-compliance...... 53

ARTICLE 31 — ACCESS RIGHTS TO RESULTS...... 53

31.1 Exercise of access rights — Waiving of access rights — No sub-licensing...... 53

31.2 Access rights for other beneficiaries, for implementing their own tasks under the action...... 53

31.3 Access rights for other beneficiaries, for exploiting their own results...... 53

31.4 Access rights of affiliated entities...... 53

31.5 Access rights for the EU institutions, bodies, offices or agencies and EU Member States...... 53

31.6 Access rights for third parties...... 54

31.7 Consequences of non-compliance...... 54

SECTION 4 OTHER RIGHTS AND OBLIGATIONS...... 54

ARTICLE 32 — RECRUITMENT AND WORKING CONDITIONS FOR RESEARCHERS...... 54

32.1 Obligation to take measures to implement the European Charter for Researchers and Code of Conduct for the Recruitment of Researchers...... 54

32.2 Consequences of non-compliance...... 54

ARTICLE 33 — GENDER EQUALITY...... 54

33.1 Obligation to aim for gender equality...... 54

33.2 Consequences of non-compliance...... 55

ARTICLE 34 — ETHICS...... 55

34.1 General obligation to comply with ethical principles...... 55

34.2 Activities raising ethical issues...... 55

34.3 Activities involving human embryos or human embryonic stem cells...... 56

34.4 Consequences of non-compliance...... 56

ARTICLE 35 — CONFLICT OF INTERESTS...... 56

35.1 Obligation to avoid a conflict of interests...... 56

35.2 Consequences of non-compliance...... 56

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ARTICLE 36 — CONFIDENTIALITY...... 56

36.1 General obligation to maintain confidentiality...... 56

36.2 Consequences of non-compliance...... 57

ARTICLE 37 — SECURITY-RELATED OBLIGATIONS...... 57

37.1 Activities raising security issues...... 57

37.2 Classified deliverables...... 58

37.3 Activities involving dual-use goods or dangerous materials and substances...... 58

37.4 Consequences of non-compliance...... 58

ARTICLE 38 — PROMOTING THE ACTION — VISIBILITY OF EU FUNDING...... 58

38.1 Communication activities by beneficiaries...... 58

38.2 Communication activities by the Agency...... 59

38.3 Consequences of non-compliance...... 60

ARTICLE 39 — PROCESSING OF PERSONAL DATA...... 60

39.1 Processing of personal data by the Agency...... 60

39.2 Processing of personal data by the beneficiaries...... 60

39.3 Consequences of non-compliance...... 61

ARTICLE 40 — ASSIGNMENTS OF CLAIMS FOR PAYMENT AGAINST THE AGENCY...... 61

CHAPTER 5 DIVISION OF BENEFICIARIES’ ROLES AND RESPONSIBILITIES ...... 61

ARTICLE 41 — DIVISION OF BENEFICIARIES’ ROLES AND RESPONSIBILITIES ...... 61

41.1 Roles and responsibilities towards the Agency...... 61

41.2 Internal division of roles and responsibilities...... 61

41.3 Internal arrangements between beneficiaries — Consortium agreement...... 62

41.4 Relationship with complementary beneficiaries — Collaboration agreement...... 63

41.5 Relationship with partners of a joint action — Coordination agreement...... 63

CHAPTER 6 REJECTION OF COSTS — REDUCTION OF THE GRANT — RECOVERY — PENALTIES — DAMAGES — SUSPENSION — TERMINATION — FORCE MAJEURE...... 63

SECTION 1 REJECTION OF COSTS — REDUCTION OF THE GRANT — RECOVERY — PENALTIES...... 63

ARTICLE 42 — REJECTION OF INELIGIBLE COSTS...... 63

42.1 Conditions...... 63

42.2 Ineligible costs to be rejected — Calculation — Procedure...... 63

42.3 Effects...... 63

ARTICLE 43 — REDUCTION OF THE GRANT...... 64

43.1 Conditions...... 64

43.2 Amount to be reduced — Calculation — Procedure...... 64

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43.3 Effects...... 64

ARTICLE 44 — RECOVERY OF UNDUE AMOUNTS...... 65

44.1 Amount to be recovered — Calculation — Procedure...... 65

ARTICLE 45 — ADMINISTRATIVE AND FINANCIAL PENALTIES...... 69

45.1 Conditions...... 69

45.2 Duration — Amount of penalty — Calculation...... 69

45.3 Procedure...... 69

SECTION 2 LIABILITY FOR DAMAGES...... 70

ARTICLE 46 — LIABILITY FOR DAMAGES...... 70

46.1 Liability of the Agency...... 70

46.2 Liability of the beneficiaries...... 70

SECTION 3 SUSPENSION AND TERMINATION...... 71

ARTICLE 47 — SUSPENSION OF PAYMENT DEADLINE...... 71

47.1 Conditions...... 71

47.2 Procedure...... 71

ARTICLE 48 — SUSPENSION OF PAYMENTS...... 72

48.1 Conditions...... 72

48.2 Procedure...... 72

ARTICLE 49 — SUSPENSION OF THE ACTION IMPLEMENTATION...... 73

49.1 Suspension of the action implementation, by the beneficiaries...... 73

49.2 Suspension of the action implementation, by the Agency...... 73

ARTICLE 50 — TERMINATION OF THE AGREEMENT OR OF PARTICIPATION FOR ONE OR MORE BENEFICIARIES...... 74

50.1 Termination of the Agreement by the beneficiaries...... 74

50.2 Termination of participation for one or more beneficiaries, by the beneficiaries...... 75

50.3 Termination of the Agreement or participation for one or more beneficiaries, by the Agency...... 77

SECTION 4 FORCE MAJEURE...... 81

ARTICLE 51 — FORCE MAJEURE...... 81

51.1 Force majeure...... 81

CHAPTER 7 FINAL PROVISIONS...... 82

ARTICLE 52 — COMMUNICATIONS BETWEEN THE PARTIES...... 82

52.1 Form and means of communication...... 82

52.2 Date of communication...... 82

52.3 Addresses for communication...... 83

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ARTICLE 53 — INTERPRETATION OF THE AGREEMENT...... 83

53.1 Precedence of the Terms and Conditions over the Annexes...... 83

53.2 Privileges and immunities...... 83

ARTICLE 54 — CALCULATION OF PERIODS, DATES AND DEADLINES...... 83

ARTICLE 55 — AMENDMENTS TO THE AGREEMENT...... 83

55.1 Conditions...... 83

55.2 Procedure...... 84

ARTICLE 56 — ACCESSION TO THE AGREEMENT...... 84

56.1 Accession of the beneficiaries mentioned in the Preamble...... 84

56.2 Addition of new beneficiaries...... 84

ARTICLE 57 — APPLICABLE LAW AND SETTLEMENT OF DISPUTES...... 85

57.1 Applicable law...... 85

57.2 Dispute settlement...... 85

ARTICLE 58 — ENTRY INTO FORCE OF THE AGREEMENT...... 85

12 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

CHAPTER 1 GENERAL

ARTICLE 1 — SUBJECT OF THE AGREEMENT

This Agreement sets out the rights and obligations and the terms and conditions applicable to the grant awarded to the beneficiaries for implementing the action set out in Chapter 2.

CHAPTER 2 ACTION

ARTICLE 2 — ACTION TO BE IMPLEMENTED

The grant is awarded for the action entitled ‘DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS — TREASURE’ (‘action’), as described in Annex 1.

ARTICLE 3 — DURATION AND STARTING DATE OF THE ACTION

The duration of the action will be 48 months as of 01/04/2015 (‘starting date of the action’).

ARTICLE 4 — ESTIMATED BUDGET AND BUDGET TRANSFERS

4.1 Estimated budget

The ‘estimated budget’ for the action is set out in Annex 2.

It contains the estimated eligible costs and the forms of costs, broken down by beneficiary (and linked third party) and budget category (see Articles 5, 6, and 14).

4.2 Budget transfers

The estimated budget breakdown indicated in Annex 2 may be adjusted by transfers of amounts between beneficiaries or between budget categories (or both). This does not require an amendment according to Article 55, if the action is implemented as described in Annex 1.

The beneficiaries may not however:

- add costs relating to subcontracts not provided for in Annex 1, unless such additional subcontracts are approved in accordance with Article 13.

CHAPTER 3 GRANT

ARTICLE 5 — GRANT AMOUNT, FORM OF GRANT, REIMBURSEMENT RATES AND FORMS OF COSTS

5.1 Maximum grant amount

The ‘maximum grant amount’ is EUR 3,395,986.75 (three million three hundred and ninety five thousand nine hundred and eighty six EURO and seventy five eurocents).

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5.2 Form of grant, reimbursement rates and forms of costs

The grant reimburses 100% of the action's eligible costs (see Article 6) (‘reimbursement of eligible costs grant’) (see Annex 2).

The estimated eligible costs of the action are EUR 3,395,987.00 (three million three hundred and ninety five thousand nine hundred and eighty seven EURO).

Eligible costs (see Article 6) must be declared under the following forms ('forms of costs'):

(a) for direct personnel costs:

- as actually incurred costs (‘actual costs’) or

- on the basis of an amount per unit calculated by the beneficiary in accordance with its usual cost accounting practices (‘unit costs’).

Personnel costs for SME owners or beneficiaries that are natural persons not receiving a salary (see Article 6.2, Points A.4 and A.5) must be declared on the basis of the amount per unit set out in Annex 2 (unit costs);

(b) for direct costs for subcontracting: as actually incurred costs (actual costs);

(d) for other direct costs: as actually incurred costs (actual costs);

(e) for indirect costs: on the basis of a flat-rate applied as set out in Article 6.2, Point E (‘flat- rate costs’);

5.3 Final grant amount — Calculation

The ‘final grant amount’ depends on the actual extent to which the action is implemented in accordance with the Agreement’s terms and conditions.

This amount is calculated by the Agency — when the payment of the balance is made (see Article 21.4) — in the following steps:

Step 1 – Application of the reimbursement rates to the eligible costs

Step 2 – Limit to the maximum grant amount

Step 3 – Reduction due to the no-profit rule

Step 4 – Reduction due to improper implementation or breach of other obligations

5.3.1 Step 1 — Application of the reimbursement rates to the eligible costs

The reimbursement rate(s) (see Article 5.2) are applied to the eligible costs (actual costs, unit costs and flat-rate costs; see Article 6) declared by the beneficiaries and linked third parties (see Article 20) and approved by the Agency (see Article 21).

14 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

5.3.2 Step 2 — Limit to the maximum grant amount

If the amount obtained following Step 1 is higher than the maximum grant amount set out in Article 5.1, it will be limited to the latter.

5.3.3 Step 3 — Reduction due to the no-profit rule

The grant must not produce a profit.

‘Profit’ means the surplus of the amount obtained following Steps 1 and 2 plus the action’s total receipts, over the action’s total eligible costs.

The ‘action’s total eligible costs’ are the consolidated total eligible costs approved by the Agency.

The ‘action’s total receipts’ are the consolidated total receipts generated during its duration (see Article 3).

The following are considered receipts:

(a) income generated by the action; if the income is generated from selling equipment or other assets purchased under the Agreement, the receipt is up to the amount declared as eligible under the Agreement;

(b) financial contributions given by third parties to the beneficiary or to a linked third party specifically to be used for the action, and

(c) in-kind contributions provided by third parties free of charge and specifically to be used for the action, if they have been declared as eligible costs.

The following are however not considered receipts:

(a) income generated by exploiting the action’s results (see Article 28);

(b) financial contributions by third parties, if they may be used to cover costs other than the eligible costs (see Article 6);

(c) financial contributions by third parties with no obligation to repay any amount unused at the end of the period set out in Article 3.

If there is a profit, it will be deducted from the amount obtained following Steps 1 and 2.

5.3.4 Step 4 — Reduction due to improper implementation or breach of other obligations — Reduced grant amount — Calculation

If the grant is reduced (see Article 43), the Agency will calculate the reduced grant amount by deducting the amount of the reduction (calculated in proportion to the improper implementation of the action or to the seriousness of the breach of obligations in accordance with Article 43.2) from the maximum grant amount set out in Article 5.1.

The final grant amount will be the lower of the following two:

- the amount obtained following Steps 1 to 3 or

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- the reduced grant amount following Step 4.

5.4 Revised final grant amount — Calculation

If — after the payment of the balance (in particular, after checks, reviews, audits or investigations; see Article 22) — the Agency rejects costs (see Article 42) or reduces the grant (see Article 43), it will calculate the ‘revised final grant amount’ for the beneficiary concerned by the findings.

This amount is calculated by the Agency on the basis of the findings, as follows:

- in case of rejection of costs: by applying the reimbursement rate to the revised eligible costs approved by the Agency for the beneficiary concerned;

- in case of reduction of the grant: by calculating the concerned beneficiary’s share in the grant amount reduced in proportion to its improper implementation of the action or to the seriousness of its breach of obligations (see Article 43.2).

In case of rejection of costs and reduction of the grant, the revised final grant amount for the beneficiary concerned will be the lower of the two amounts above.

ARTICLE 6 — ELIGIBLE AND INELIGIBLE COSTS

6.1 General conditions for costs to be eligible

‘Eligible costs’ are costs that meet the following criteria:

(a) for actual costs:

(i) they must be actually incurred by the beneficiary;

(ii) they must be incurred in the period set out in Article 3, with the exception of costs relating to the submission of the periodic report for the last reporting period and the final report (see Article 20);

(iii) they must be indicated in the estimated budget set out in Annex 2;

(iv) they must be incurred in connection with the action as described in Annex 1 and necessary for its implementation;

(v) they must be identifiable and verifiable, in particular recorded in the beneficiary’s accounts in accordance with the accounting standards applicable in the country where the beneficiary is established and with the beneficiary’s usual cost accounting practices;

(vi) they must comply with the applicable national law on taxes, labour and social security, and

(vii) they must be reasonable, justified and must comply with the principle of sound financial management, in particular regarding economy and efficiency.

(b) for unit costs:

(i) they must be calculated as follows:

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{amounts per unit set out in Annex 2 or calculated by the beneficiary in accordance with its usual cost accounting practices (see Article 6.2, PointA)}

multiplied by

{the number of actual units};

(ii) the number of actual units must comply with the following conditions:

- the units must be actually used or produced in the period set out in Article 3;

- the units must be necessary for implementing the action or produced by it, and

- the number of units must be identifiable and verifiable, in particular supported by records and documentation (see Article 18).

(i) they must be calculated by applying the flat-rate set out in Annex 2, and

(ii) the costs (actual costs or unit costs) to which the flat-rate is applied must comply with the conditions for eligibility set out in this Article.

6.2 Specific conditions for costs to be eligible

Costs are eligible if they comply with the general conditions (see above) and the specific conditions set out below for each of the following budget categories:

A. direct personnel costs;

B. direct costs of subcontracting;

C. not applicable;

D. other direct costs;

E. indirect costs;

‘Direct costs’ are costs that are directly linked to the action implementation and can therefore be attributed to it directly. They must not include any indirect costs (see Point E below).

‘Indirect costs’ are costs that are not directly linked to the action implementation and therefore cannot be attributed directly to it.

A. Direct personnel costs

Types of eligible personnel costs

A.1 Personnel costs are eligible if they are related to personnel working for the beneficiary under an employment contract (or equivalent appointing act) and assigned to the action. They must be limited to salaries (including during parental leave), social security contributions, taxes and other

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costs included in the remuneration, if they arise from national law or the employment contract (or equivalent appointing act).

Beneficiaries that are non-profit legal entities2 may also declare as personnel costs additional remuneration for personnel assigned to the action (including payments on the basis of supplementary contracts regardless of their nature), if:

(a) it is part of the beneficiary’s usual remuneration practices and is paid in a consistent manner whenever the same kind of work or expertise is required;

(b) the criteria used to calculate the supplementary payments are objective and generally applied by the beneficiary, regardless of the source of funding used.

Additional remuneration for personnel assigned to the action is eligible up to the following amount:

(a) if the person works full time and exclusively on the action during the full year: up to EUR 8 000;

(b) if the person works exclusively on the action but not full-time or not for the full year: up to the corresponding pro-rata amount of EUR 8 000, or

{{EUR 8 000

divided by

the number of annual productive hours (see below)},

multiplied by

the number of hours that the person has worked on the action during the year}.

A.2 The costs for natural persons working under a direct contract with the beneficiary other than an employment contract are eligible personnel costs, if:

(a) the person works under the beneficiary’s instructions and, unless otherwise agreed with the beneficiary, on the beneficiary’s premises;

(b) the result of the work carried out belongs to the beneficiary, and

(c) the costs are not significantly different from those for personnel performing similar tasks under an employment contract with the beneficiary.

A.3 The costs of personnel seconded by a third party against payment are eligible personnel costs, if the conditions in Article 11 are met.

2 For the definition, see Article 2.1(14) of the Rules for Participation Regulation No 1290/2013: ‘non-profit legal entity’ means a legal entity which by its legal form is non-profit-making or which has a legal or statutory obligation not to distribute profits to its shareholders or individual members.

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A.4 Costs owners of beneficiaries that are small and medium-sized enterprises (‘SME owners’) who are working on the action and who do not receive a salary are eligible personnel costs, if they correspond to the amount per unit set out in Annex 2 multiplied by the number of actual hours worked on the action.

A.5 Costs of ‘beneficiaries that are natural persons’ not receiving a salary are eligible personnel costs, if they correspond to the amount per unit set out in Annex 2 multiplied by the number of actual hours worked on the action.

Calculation

Personnel costs must be calculated by the beneficiaries as follows:

{{hourly rate

multiplied by

the number of actual hours worked on the action},

plus

for non-profit legal entities: additional remuneration to personnel assigned to the action under the conditions set out above (Point A.1)}.

The number of actual hours declared for a person must be identifiable and verifiable (see Article 18).

The total number of hours declared in EU or Euratom grants, for a person for a year, cannot be higher than the annual productive hours used for the calculations of the hourly rate:

{the number of annual productive hours for the year (see below)

minus

total number of hours declared by the beneficiary for that person in that year for other EU or Euratom grants}.

The ‘hourly rate’ is one of the following:

(a) for personnel costs declared as actual costs: the hourly rate is the amount calculated as follows:

{actual annual personnel costs (excluding additional remuneration) for the person

divided by

number of annual productive hours}.

The beneficiaries must use the annual personnel costs and the number of annual productive hours for each financial year covered by the reporting period. If a financial year is not closed at the end of the reporting period, the beneficiaries must use the hourly rate of the last closed financial year available.

For the ‘number of annual productive hours’, the beneficiaries may choose one of the following:

(i) 1 720 hours for persons working full time (or corresponding pro-rata for persons not working full time);

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(ii) the total number of hours worked by the person in the year for the beneficiary, calculated as follows:

{annual workable hours of the person (according to the employment contract, applicable labour agreement or national law)

plus

overtime worked

minus

absences (such as sick leave and special leave)}.

‘Annual workable hours’ means the period during which the personnel must be working, at the employer’s disposal and carrying out his/her activity or duties under the employment contract, applicable collective labour agreement or national working time legislation.

If the contract (or applicable collective labour agreement or national working time legislation) does not allow to determine the annual workable hours, this option cannot be used;

(iii) the ‘standard number of annual hours’ generally applied by the beneficiary for its personnel in accordance with its usual cost accounting practices. This number must be at least 90% of the ‘standard annual workable hours’.

If there is no applicable reference for the standard annual workable hours, this option cannot be used.

For all options, the actual time spent on parental leave by a person assigned to the action may be deducted from the number of annual productive hours;

(b) for personnel costs declared on the basis of unit costs: the hourly rate is one of the following:

(i) for SME owners or beneficiaries that are natural persons: the hourly rate set out in Annex 2 (see Points A.4 and A.5 above), or

(ii) for personnel costs declared on the basis of the beneficiary’s usual cost accounting practices: the hourly rate calculated by the beneficiary in accordance with its usual cost accounting practices, if:

- the cost accounting practices used are applied in a consistent manner, based on objective criteria, regardless of the source of funding;

- the hourly rate is calculated using the actual personnel costs recorded in the beneficiary’s accounts, excluding any ineligible cost or costs included in other budget categories.

The actual personnel costs may be adjusted by the beneficiary on the basis of budgeted or estimated elements. Those elements must be relevant for calculating

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the personnel costs, reasonable and correspond to objective and verifiable information, and

- the hourly rate is calculated using the number of annual productive hours (see above).

B. Direct costs of subcontracting (including related duties, taxes and charges such as non-deductible value added tax (VAT) paid by the beneficiary) are eligible if the conditions in Article 13 are met.

C. Direct costs of providing financial support to third parties not applicable.

D. Other direct costs

D.1 Travel costs and related subsistence allowances (including related duties, taxes and charges such as non-deductible value added tax (VAT) paid by the beneficiary) are eligible if they are in line with the beneficiary’s usual practices on travel.

D.2 The depreciation costs of equipment, infrastructure or other assets (new or second-hand) as recorded in the beneficiary’s accounts are eligible, if they were purchased in accordance with Article 10 and written off in accordance with international accounting standards and the beneficiary’s usual accounting practices.

The costs of renting or leasing equipment, infrastructure or other assets (including related duties, taxes and charges such as non-deductible value added tax (VAT) paid by the beneficiary) are also eligible, if they do not exceed the depreciation costs of similar equipment, infrastructure or assets and do not include any financing fees.

The costs of equipment, infrastructure or other assets contributed in-kind against payment are eligible, if they do not exceed the depreciation costs of similar equipment, infrastructure or assets, do not include any financing fees and if the conditions in Article 11 are met.

The only portion of the costs that will be taken into account is that which corresponds to the duration of the action and rate of actual use for the purposes of the action.

D.3 Costs of other goods and services (including related duties, taxes and charges such as non- deductible value added tax (VAT) paid by the beneficiary) are eligible, if they are:

(a) purchased specifically for the action and in accordance with Article 10 or

(b) contributed in kind against payment and in accordance with Article 11.

Such goods and services include, for instance, consumables and supplies, dissemination (including open access), protection of results, certificates on the financial statements (if they are required by the Agreement), certificates on the methodology, translations and publications.

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D.4 The capitalised and operating costs of ‘large research infrastructure’ 3 directly used for the action are eligible, if:

(a) the value of the large research infrastructure represents at least 75% of the total fixed assets (at historical value in its last closed balance sheet before the date of the signature of the Agreement or as determined on the basis of the rental and leasing costs of the research infrastructure 4);

(b) the beneficiary’s methodology for declaring the costs for large research infrastructure has been positively assessed by the Commission (‘ex-ante assessment’);

(c) the beneficiary declares as direct eligible costs only the portion which corresponds to the duration of the action and the rate of actual use for the purposes of the action, and

(d) they comply with the conditions as further detailed in the Horizon 2020 Grant Manual.

E. Indirect costs

Indirect costs are eligible if they are declared on the basis of the flat-rate of 25% of the eligible direct costs (see Article 5.2 and Points A to D above), from which are excluded:

(a) costs of subcontracting and

(b) costs of in-kind contributions provided by third parties which are not used on the beneficiary’s premises.

Beneficiaries receiving an operating grant5 financed by the EU or Euratom budget cannot declare indirect costs for the period covered by the operating grant.

3 ‘Large research infrastructure’ means research infrastructure of a total value of at least EUR 20 million, for a beneficiary, calculated as the sum of historical asset values of each individual research infrastructure of that beneficiary, as they appear in its last closed balance sheet before the date of the signature of the Agreement or as determined on the basis of the rental and leasing costs of the research infrastructure. 4 For the definition, see Article 2(6) of Regulation (EU) No 1291/2013 of the European Parliament and of the Council of 11 December 2013 establishing Horizon 2020 - the Framework Programme for Research and Innovation (2014-2020) (OJ L 347, 20.12.2013 p.104)-(‘Horizon 2020 Framework Programme Regulation No 1291/2013’): ‘Research infrastructure’ are facilities, resources and services that are used by the research communities to conduct research and foster innovation in their fields. Where relevant, they may be used beyond research, e.g. for education or public services. They include: major scientific equipment (or sets of instruments); knowledge-based resources such as collections, archives or scientific data; e-infrastructures such as data and computing systems and communication networks; and any other infrastructure of a unique nature essential to achieve excellence in research and innovation. Such infrastructures may be ‘single-sited’, ‘virtual’ or ‘distributed’. 5 For the definition, see Article 121(1)(b) of Regulation (EU, Euratom) No 966/2012 of the European Parliament and of the Council of 25 October 2012 on the financial rules applicable to the general budget of the Union and repealing Council Regulation (EC, Euratom) No 1605/2002 (OJ L 218, 26.10.2012, p.1) (‘Financial Regulation No 966/2012’): ‘operating grant’ means direct financial contribution, by way of donation, from the budget in order to finance the functioning of a body which pursues an aim of general EU interest or has an objective forming part of and supporting an EU policy.

22 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

6.3 Conditions for costs of linked third parties to be eligible

Costs incurred by linked third parties are eligible if they fulfil — mutatis mutandis — the general and specific conditions for eligibility set out in this Article (Article 6.1 and 6.2) and Article 14.

6.4 Conditions for in-kind contributions provided by third parties free of charge to be eligible

In-kind contributions provided free of charge are eligible direct costs (for the beneficiary or linked third party), if the costs incurred by the third party fulfil — mutatis mutandis — the general and specific conditions for eligibility set out in this Article (Article 6.1 and 6.2) and Article 12.

6.5 Ineligible costs

‘Ineligible costs’ are:

(a) costs that do not comply with the conditions set out above (Article 6.1 to 6.4), in particular:

(i) costs related to return on capital;

(ii) debt and debt service charges;

(iii) provisions for future losses or debts;

(iv) interest owed;

(v) doubtful debts;

(vi) currency exchange losses;

(vii) bank costs charged by the beneficiary’s bank for transfers from the Agency;

(viii)excessive or reckless expenditure;

(ix) deductible VAT;

(x) costs incurred during suspension of the implementation of the action (see Article 49);

(b) costs declared under another EU or Euratom grant (including grants awarded by a Member State and financed by the EU or Euratom budget and grants awarded by bodies other than the Agency for the purpose of implementing the EU or Euratom budget); in particular, indirect costs if the beneficiary is already receiving an operating grant financed by the EU or Euratom budget in the same period.

6.6 Consequences of declaration of ineligible costs

Declared costs that are ineligible will be rejected (see Article 42).

This may also lead to any of the other measures described in Chapter 6.

CHAPTER 4 RIGHTS AND OBLIGATIONS OF THE PARTIES

23 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

SECTION 1 RIGHTS AND OBLIGATIONS RELATED TO IMPLEMENTING THE ACTION

ARTICLE 7 — GENERAL OBLIGATION TO PROPERLY IMPLEMENT THE ACTION

7.1 General obligation to properly implement the action

The beneficiaries must implement the action as described in Annex 1 and in compliance with the provisions of the Agreement and all legal obligations under applicable EU, international and national law.

7.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 8 — RESOURCES TO IMPLEMENT THE ACTION

The beneficiaries must have the appropriate resources to implement the action.

If it is necessary to implement the action, the beneficiaries may:

- purchase goods, works and services (see Article 10);

- use in-kind contributions provided by third parties against payment (see Article 11);

- use in-kind contributions provided by third parties free of charge (see Article 12);

- call upon subcontractors to implement action tasks described in Annex 1 (see Article 13);

- call upon linked third parties to implement action tasks described in Annex 1 (see Article 14).

In these cases, the beneficiaries retain sole responsibility towards the Agency and the other beneficiaries for implementing the action.

ARTICLE 9 — IMPLEMENTATION OF ACTION TASKS BY BENEFICIARIES NOT RECEIVING EU FUNDING

9.1 Rules for the implementation of action tasks by beneficiaries not receiving EU funding not applicable

9.2 Consequences of non-compliance not applicable

24 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

ARTICLE 10 — PURCHASE OF GOODS, WORKS OR SERVICES

10.1 Rules for purchasing goods, works or services

10.1.1 If necessary to implement the action, the beneficiaries may purchase goods, works or services.

The beneficiaries must make such purchases ensuring the best value for money or, if appropriate, the lowest price. In doing so, they must avoid any conflict of interests (see Article 35).

The beneficiaries must ensure that the Commission and the Agency, the European Court of Auditors (ECA) and the European Anti-fraud Office (OLAF) can exercise their rights under Articles 22 and 23 also towards their contractors.

10.1.2 Beneficiaries that are ‘contracting authorities’ within the meaning of Directive 2004/18/EC6 or ‘contracting entities’ within the meaning of Directive 2004/17/EC7 must comply with the applicable national law on public procurement.

10.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under Article 10.1.1, the costs related to the contract concerned will be ineligible (see Article 6) and will be rejected (see Article 42).

If a beneficiary breaches any of its obligations under Article 10.1.2, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 11 — USE OF IN-KIND CONTRIBUTIONS PROVIDED BY THIRD PARTIES AGAINST PAYMENT

11.1 Rules for the use of in-kind contributions against payment

If necessary to implement the action, the beneficiaries may use in-kind contributions provided by third parties against payment.

The beneficiaries may declare costs related to the payment of in-kind contributions as eligible (see Article 6.1 and 6.2), up to the third parties’ costs for the seconded persons, contributed equipment, infrastructure or other assets or other contributed goods and services.

The third parties and their contributions must be set out in Annex 1. The Agency may however approve in-kind contributions not set out in Annex 1 without amendment (see Article 55), if:

- they are specifically justified in the periodic technical report and

- their use does not entail changes to the Agreement which would call into question the decision awarding the grant or breach the principle of equal treatment of applicants.

6 Directive 2004/18/EC of the European Parliament and of the Council of 31 March 2004 on the coordination of procedures for the award of public work contracts, public supply contracts and public service contracts (OJ L 134, 30.04.2004, p. 114). 7 Directive 2004/17/EC of the European Parliament and of the Council of 31 March 2004 coordinating the procurement procedures of entities operating in the water, energy, transport and postal services sectors (OJ L 134, 30.04.2004, p. 1).

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11.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the costs related to the payment of the in-kind contribution will be ineligible (see Article 6) and will be rejected (see Article 42).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 12 — USE OF IN-KIND CONTRIBUTIONS PROVIDED BY THIRD PARTIES FREE OF CHARGE

12.1 Rules for the use of in-kind contributions free of charge

If necessary to implement the action, the beneficiaries may use in-kind contributions provided by third parties free of charge.

The beneficiaries may declare costs incurred by the third parties for the seconded persons, contributed equipment, infrastructure or other assets or other contributed goods and services as eligible in accordance with Article 6.4.

The third parties and their contributions must be set out in Annex 1. The Agency may however approve in-kind contributions not set out in Annex 1 without amendment (see Article 55), if:

- they are specifically justified in the periodic technical report and

- their use does not entail changes to the Agreement which would call into question the decision awarding the grant or breach the principle of equal treatment of applicants.

12.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the costs incurred by the third parties related to the in-kind contribution will be ineligible (see Article 6) and will be rejected (see Article 42).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 13 — IMPLEMENTATION OF ACTION TASKS BY SUBCONTRACTORS

13.1 Rules for subcontracting action tasks

13.1.1 If necessary to implement the action, the beneficiaries may award subcontracts covering the implementation of certain action tasks described in Annex 1.

Subcontracting may cover only a limited part of the action.

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The beneficiaries must award the subcontracts ensuring the best value for money or, if appropriate, the lowest price. In doing so, they must avoid any conflict of interests (see Article 35).

The tasks to be implemented and the estimated cost for each subcontract must be set out in Annex 1 and the total estimated costs of subcontracting per beneficiary must be set out in Annex 2. The Agency may however approve subcontracts not set out in Annex 1 and 2 without amendment (see Article 55), if:

- they are specifically justified in the periodic technical report and

- they do not entail changes to the Agreement which would call into question the decision awarding the grant or breach the principle of equal treatment of applicants.

13.1.2 The beneficiaries must ensure that their obligations under Articles 35, 36, 38 and 46 also apply to the subcontractors.

Beneficiaries that are ‘contracting authorities’ within the meaning of Directive 2004/18/EC or ‘contracting entities’ within the meaning of Directive 2004/17/EC must comply with the applicable national law on public procurement.

13.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under Article 13.1.1, the costs related to the subcontract concerned will be ineligible (see Article 6) and will be rejected (see Article 42).

If a beneficiary breaches any of its obligations under Article 13.1.2, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

27 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

ARTICLE 14 — IMPLEMENTATION OF ACTION TASKS BY LINKED THIRD PARTIES

14.1 Rules for calling upon linked third parties to implement part of the action

14.1.1 The following affiliated entities9 and third parties with a legal link to a beneficiary10 (‘linked third parties’) may implement the action tasks attributed to them in Annex 1:

- DRUSTVO REJCEV KRSKOPOLJSKIH PRASICEV, affiliated or linked to KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO

- BAUERLICHE ERZEUGERGEMEINSCHAFT SCHWABISCH HALL AG, affiliated or linked to BAUERLICHE ERZEUGERGEMEINSCHAFT SCHWABISCH HALL WV

- ZEMLJISNA ZAJEDNICA PLEMENITA OPCINA TUROPOLJSKA, affiliated or linked to SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET

- POLJOPRIVREDNO-USLUZNA ZADRUGA LETA, affiliated or linked to SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU

- UNIVERSITA DEGLI STUDI DI SASSARI, affiliated or linked to AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA

- CONSORZIO DI TUTELA DELLA CINTA SENESE, affiliated or linked to UNIVERSITA DEGLI STUDI DI FIRENZE

- LIETUVOS NYKSTANCIU UKINIU GYVUNU AUGINTOJU ASOCIACIJA, affiliated or linked to LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS

- ANCSUB, affiliated or linked to INSTITUTO POLITECNICO DE VIANA DE CASTELO

- ASSOCIACAO NACIONAL DOS CRIADORES DO PORCO ALENTEJANO, affiliated or linked to UNIVERSIDADE DE EVORA

- EKO SELO, affiliated or linked to INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN

- INSTITUT ZA HIGIJENU I TEHNOLOGIJUMESA DRUSTVENO PREDUZECE, affiliated or linked to FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE

9 For the definition, see Article 2.1(2) of the Rules for Participation Regulation No 1290/2013: 'affiliated entity' means any legal entity that is under the direct or indirect control of a participant, or under the same direct or indirect control as the participant, or that is directly or indirectly controlling a participant. ‘Control’ may take any of the following forms: (a) the direct or indirect holding of more than 50% of the nominal value of the issued share capital in the legal entity concerned, or of a majority of the voting rights of the shareholders or associates of that entity; (b) the direct or indirect holding, in fact or in law, of decision-making powers in the legal entity concerned. However the following relationships between legal entities shall not in themselves be deemed to constitute controlling relationships: (a) the same public investment corporation, institutional investor or venture-capital company has a direct or indirect holding of more than 50% of the nominal value of the issued share capital or a majority of voting rights of the shareholders or associates; (b) the legal entities concerned are owned or supervised by the same public body. 10 ‘Third party with a legal link to a beneficiary’ is any legal entity which has a legal link to the beneficiary implying collaboration that is not limited to the action.

28 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

- UNIVERSIDAD DE EXTREMADURA, affiliated or linked to INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA

The linked third parties may declare as eligible the costs they incur for implementing the action tasks in accordance with Article 6.3.

14.1.2 The beneficiaries must ensure that their obligations under Articles 18, 20, 35, 36 and 38 also apply to their linked third parties.

14.2 Consequences of non-compliance

If any obligation under Article 14.1.1 is breached, the costs of the third party will be ineligible (see Article 6) and will be rejected (see Article 42).

If any obligation under Article 14.1.2 is breached, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 15 — FINANCIAL SUPPORT TO THIRD PARTIES

15.1 Rules for providing financial support to third parties not applicable

15.2 Financial support in the form of prizes not applicable

15.3 Consequences of non-compliance not applicable

ARTICLE 16 — PROVISION OF TRANS-NATIONAL OR VIRTUAL ACCESS TO RESEARCH INFRASTRUCTURE

16.1 Rules for providing trans-national access to research infrastructure not applicable

16.2 Rules for providing virtual access to research infrastructure not applicable

16.3 Consequences of non-compliance not applicable

29 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

SECTION 2 RIGHTS AND OBLIGATIONS RELATED TO THE GRANT ADMINISTRATION

ARTICLE 17 — GENERAL OBLIGATION TO INFORM

17.1 Obligation to provide information upon request

The beneficiaries must provide — during implementation of the action or afterwards — any information requested in order to verify proper implementation of the action and compliance with the obligations under the Agreement (see Article 41.2).

17.2 Obligation to keep information up to date and to inform about events and circumstances likely to affect the Agreement

Each beneficiary must keep information stored in the 'Beneficiary Register' (in the electronic exchange system; see Article 52) up to date, in particular, its name, address, legal representatives, legal form and organisation type.

Each beneficiary must immediately inform the coordinator — which must immediately inform the Agency and the other beneficiaries — of any of the following:

(a) events which are likely to affect significantly or delay the implementation of the action or the EU's financial interests, in particular:

(i) changes in its legal, financial, technical, organisational or ownership situation or those of its linked third parties and

(ii) changes in the name, address, legal form, organisation type of its linked third parties;

(b) circumstances affecting:

(i) the decision to award the grant or

(ii) compliance with requirements under the Agreement.

17.3 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 18 — KEEPING RECORDS — SUPPORTING DOCUMENTATION

18.1 Obligation to keep records and other supporting documentation

The beneficiaries must — for a period of five years after the payment of the balance — keep records and other supporting documentation in order to prove the proper implementation of the action and the costs they declare as eligible.

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They must make them available upon request (see Article 17) or in the context of checks, reviews, audits or investigations (see Article 22).

If there are on-going checks, reviews, audits, investigations, litigation or other pursuits of claims under the Agreement (including the extension of findings; see Articles 22), the beneficiaries must keep the records and other supporting documentation until the end of these procedures.

The beneficiaries must keep the original documents. Digital and digitalised documents are considered originals if they are authorised by the applicable national law. The Agency may accept non-original documents if it considers that they offer a comparable level of assurance.

18.1.1 Records and other supporting documentation on the scientific and technical implementation

The beneficiaries must keep records and other supporting documentation on scientific and technical implementation of the action in line with the accepted standards in the respective field.

18.1.2 Records and other documentation to support the costs declared

The beneficiaries must keep the records and documentation supporting the costs declared, in particular the following:

(a) for actual costs: adequate records and other supporting documentation to prove the costs declared, such as contracts, subcontracts, invoices and accounting records. In addition, the beneficiaries' usual cost accounting practices and internal control procedures must enable direct reconciliation between the amounts declared, the amounts recorded in their accounts and the amounts stated in the supporting documentation;

(b) for unit costs: adequate records and other supporting documentation to prove the number of units declared. Beneficiaries do not need to identify the actual eligible costs covered or to keep or provide supporting documentation (such as accounting statements) to prove the amount per unit.

In addition, for direct personnel costs declared as unit costs calculated in accordance with the beneficiary's usual cost accounting practices, the beneficiaries must keep adequate records and documentation to prove that the cost accounting practices used comply with the conditions set out in Article 6.2, Point A.

The beneficiaries and linked third parties may submit to the Agency, for approval, a certificate (drawn up in accordance with Annex 6) stating that their usual cost accounting practices comply with these conditions (‘certificate on the methodology’). If the certificate is approved, costs declared in line with this methodology will not be challenged subsequently, unless the beneficiaries have concealed information for the purpose of the approval.

(c) for flat-rate costs: adequate records and other supporting documentation to prove the eligibility of the costs to which the flat-rate is applied. The beneficiaries do not need to identify the costs covered or provide supporting documentation (such as accounting statements) to prove the amount declared at a flat-rate.

In addition, for personnel costs (declared as actual costs or on the basis of unit costs), the beneficiaries must keep time records for the number of hours declared. The time records must be in writing and

31 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2 approved by the persons working on the action and their supervisors, at least monthly. In the absence of reliable time records of the hours worked on the action, the Agency may accept alternative evidence supporting the number of hours declared, if it considers that it offers an adequate level of assurance.

As an exception, for persons working exclusively on the action, there is no need to keep time records, if the beneficiary signs a declaration confirming that the persons concerned have worked exclusively on the action.

For costs declared by linked third parties (see Article 14), it is the beneficiary that must keep the originals of the financial statements and the certificates on the financial statements of the linked third parties.

18.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, costs insufficiently substantiated will be ineligible (see Article 6) and will be rejected (see Article 42), and the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 19 — SUBMISSION OF DELIVERABLES

19.1 Obligation to submit deliverables

The coordinator must submit the ‘deliverables’ identified in Annex 1, in accordance with the timing and conditions set out in it.

19.2 Consequences of non-compliance

If the coordinator breaches any of its obligations under this Article, the Agency may apply any of the measures described in Chapter 6.

ARTICLE 20 — REPORTING — PAYMENT REQUESTS

20.1 General obligation to submit reports

The coordinator must submit to the Agency (see Article 52) technical and financial reports, including requests for payment.

The reports must be drawn up using the forms and templates provided by the Agency in the electronic exchange system (see Article 52).

20.2 Reporting periods

The action is divided into the following ‘reporting periods’:

- RP1: from month 1 to month 18 - RP2: from month 19 to month 36 - RP3: from month 37 to the last month of the project

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20.3 Periodic reports — Requests for interim payments

The coordinator must submit a periodic report within 60 days following the end of each reporting period.

The periodic report must include the following:

(a) a ‘periodic technical report’ containing:

(i) an explanation of the work carried out by the beneficiaries;

(ii) an overview of the progress towards the objectives of the action, including milestones and deliverables identified in Annex 1.

This report must include explanations justifying the differences between work expected to be carried out in accordance with Annex 1 and that actually carried out.

The report must also detail the exploitation and dissemination of the results and — if required in Annex 1 — an updated ‘plan for the exploitation and dissemination of the results’;

(iii) a summary for publication by the Agency;

(iv) the answers to the ‘questionnaire’, covering issues related to the action implementation and the economic and societal impact, notably in the context of the Horizon 2020 key performance indicators and the Horizon 2020 monitoring requirements;

(b) a ‘periodic financial report’ containing:

(i) an ‘individual financial statement’ (see Annex 4) from each beneficiary and from each linked third party, for the reporting period concerned.

The individual financial statement must detail the eligible costs (actual costs, unit costs and flat-rate costs; see Article 6) for each budget category (see Annex 2).

The beneficiaries and linked third parties must declare all eligible costs, even if — for actual costs, unit costs and flat-rate costs — they exceed the amounts indicated in the estimated budget (see Annex 2). Amounts which are not declared in the individual financial statement will not be taken into account by the Agency.

If an individual financial statement is not submitted for a reporting period, it may be included in the periodic financial report for the next reporting period.

The individual financial statements of the last reporting period must also detail the receipts of the action (see Article 5.3.3).

Each beneficiary and each linked third party must certify that:

- the information provided is full, reliable and true;

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- the costs declared are eligible (see Article 6);

- the costs can be substantiated by adequate records and supporting documentation (see Article 18) that will be produced upon request (see Article 17) or in the context of checks, reviews, audits and investigations (see Article 22), and

- for the last reporting period: that all the receipts have been declared (see Article 5.3.3);

(ii) an explanation of the use of resources and the information on subcontracting (see Article 13) and in-kind contributions provided by third parties (see Articles 11 and 12) from each beneficiary and from each linked third party, for the reporting period concerned;

(iii) not applicable;

(iv) a ‘periodic summary financial statement’ (see Annex 4), created automatically by the electronic exchange system, consolidating the individual financial statements for the reporting period concerned and including — except for the last reporting period — the request for interim payment.

20.4 Final report — Request for payment of the balance

In addition to the periodic report for the last reporting period, the coordinator must submit the final report within 60 days following the end of the last reporting period.

The final report must include the following:

(a) a ‘final technical report’ with a summary for publication containing:

(i) an overview of the results and their exploitation and dissemination;

(ii) the conclusions on the action, and

(iii) the socio-economic impact of the action;

(b) a ‘final financial report’ containing:

(i) a ‘final summary financial statement’ (see Annex 4), created automatically by the electronic exchange system, consolidating the individual financial statements for all reporting periods and including the request for payment of the balance and

(ii) a ‘certificate on the financial statements’ (drawn up in accordance with Annex 5) for each beneficiary and for each linked third party, if it requests a total contribution of EUR 325 000 or more, as reimbursement of actual costs and unit costs calculated on the basis of its usual cost accounting practices (see Article 5.2 and Article 6.2, Point A).

20.5 Information on cumulative expenditure incurred not applicable

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20.6 Currency for financial statements and conversion into euro

Financial statements must be drafted in euro.

Beneficiaries and linked third parties with accounting established in a currency other than the euro must convert costs incurred in another currency into euro at the average of the daily exchange rates published in the C series of the Official Journal of the European Union, calculated over the corresponding reporting period.

If no daily euro exchange rate is published in the Official Journal of the European Union for the currency in question, it must be converted at the average of the monthly accounting rates published on the Commission’s website, calculated over the corresponding reporting period.

Beneficiaries and linked third parties with accounting established in euro must convert costs incurred in another currency into euro according to their usual accounting practices.

20.7 Language of reports

All reports (technical and financial reports, including financial statements) must be submitted in the language of the Agreement.

20.8 Consequences of non-compliance — Suspension of the payment deadline — Termination

If the reports submitted do not comply with this Article, the Agency may suspend the payment deadline (see Article 47) and apply any of the other measures described in Chapter 6.

If the coordinator breaches its obligation to submit the reports and if it fails to comply with this obligation within 30 days following a written reminder sent by the Agency, the Agreement may be terminated (see Article 50).

ARTICLE 21 — PAYMENTS AND PAYMENT ARRANGEMENTS

21.1 Payments to be made

The following payments will be made to the coordinator:

- one pre-financing payment;

- one or more interim payments, on the basis of the request(s) for interim payment (see Article 20), and

- one payment of the balance, on the basis of the request for payment of the balance (see Article 20).

21.2 Pre-financing payment — Amount — Amount retained for the Guarantee Fund

The aim of the pre-financing is to provide the beneficiaries with a float.

It remains the property of the EU until the payment of the balance.

The amount of the pre-financing payment will be EUR 1,131,995.58 (one million one hundred and thirty one thousand nine hundred and ninety five EURO and fifty eight eurocents).

35 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The Agency will — except if Article 48 applies — make the pre-financing payment to the coordinator within 30 days, either from the entry into force of the Agreement (see Article 58) or from 10 days before the starting date of the action (see Article 3), whichever is the latest.

An amount of EUR 169,799.34 (one hundred and sixty nine thousand seven hundred and ninety nine EURO and thirty four eurocents), corresponding to the 5% of the maximum grant amount (see Article 5.1), is retained by the Agency from the pre-financing payment and transferred into the ‘Guarantee Fund’.

21.3 Interim payments — Amount — Calculation

Interim payments reimburse the eligible costs incurred for the implementation of the action during the corresponding reporting periods.

The Agency will pay to the coordinator the amount due as interim payment within 90 days from receiving the periodic report (see Article 20.3), except if Articles 47 or 48 apply.

Payment is subject to the approval of the periodic report. Its approval does not imply recognition of the compliance, authenticity, completeness or correctness of its content.

The amount due as interim payment is calculated by the Agency in the following steps:

Step 1 – Application of the reimbursement rates

Step 2 – Limit to 90% of the maximum grant amount

21.3.1 Step 1 — Application of the reimbursement rates

The reimbursement rate(s) (see Article 5.2) are applied to the eligible costs (actual costs, unit costs and flat-rate costs ; see Article 6) declared by the beneficiaries and the linked third parties (see Article 20) and approved by the Agency (see above) for the concerned reporting period.

21.3.2 Step 2 — Limit to 90% of the maximum grant amount

The total amount of pre-financing and interim payments must not exceed 90% of the maximum grant amount set out in Article 5.1. The maximum amount for the interim payment will be calculated as follows:

{90% of the maximum grant amount (see Article 5.1)

minus

{pre-financing and previous interim payments}}.

21.4 Payment of the balance — Amount — Calculation — Release of the amount retained for the Guarantee Fund

The payment of the balance reimburses the remaining part of the eligible costs incurred by the beneficiaries for the implementation of the action.

If the total amount of earlier payments is greater than the final grant amount (see Article 5.3), the payment of the balance takes the form of a recovery (see Article 44).

36 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

If the total amount of earlier payments is lower than the final grant amount, the Agency will pay the balance within 90 days from receiving the final report (see Article 20.4), except if Articles 47 or 48 apply.

Payment is subject to the approval of the final report. Its approval does not imply recognition of the compliance, authenticity, completeness or correctness of its content.

The amount due as the balance is calculated by the Agency by deducting the total amount of pre- financing and interim payments (if any) already made, from the final grant amount determined in accordance with Article 5.3:

{final grant amount (see Article 5.3)

minus

{pre-financing and interim payments (if any) made}}.

At the payment of the balance, the amount retained for the Guarantee Fund (see above) will be released and:

- if the balance is positive: the amount released will be paid in full to the coordinator together with the amount due as the balance;

- if the balance is negative (payment of the balance taking the form of recovery): it will be deducted from the amount released (see Article 44.1.2). If the resulting amount:

- is positive, it will be paid to the coordinator

- is negative, it will be recovered.

The amount to be paid may however be offset — without the beneficiary’s consent — against any other amount owed to a beneficiary by the Commission or an executive agency (from the EU or Euratom budget), up to the maximum EU contribution indicated, for that beneficiary, in the estimated budget (see Annex 2).

21.5 Notification of amounts due

When making payments, the Agency will formally notify to the coordinator the amount due, specifying whether it concerns an interim payment or the payment of the balance.

For the payment of the balance, the notification will also specify the final grant amount.

In the case of reduction of the grant or recovery of undue amounts, the notification will be preceded by the contradictory procedure set out in Articles 43 and 44.

21.6 Currency for payments

The Agency will make all payments in euro.

21.7 Payments to the coordinator — Distribution to the beneficiaries

Payments will be made to the coordinator.

37 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

Payments to the coordinator will discharge the Agency from its payment obligation.

The coordinator must distribute the payments between the beneficiaries without unjustified delay.

Pre-financing may however be distributed only:

(a) if the minimum number of beneficiaries set out in the call for proposals has acceded to the Agreement (see Article 56) and

(b) to beneficiaries that have acceded to the Agreement (see Article 56).

21.8 Bank account for payments

All payments will be made to the following bank account:

Name of bank: BANK OF SLOVENIA Address of branch: 35, SLOVENSKA LJUBLJANA, Slovenia Full name of the account holder: KMETIJSKI INSTITUT SLOVENIJEAGRICULTURAL INSTITUTE OF SLOVENIA Full account number (including bank codes): IBAN code: SI56011006030348219

21.9 Costs of payment transfers

The cost of the payment transfers is borne as follows:

- the Agency bears the cost of transfers charged by its bank;

- the beneficiary bears the cost of transfers charged by its bank;

- the party causing a repetition of a transfer bears all costs of the repeated transfer.

21.10 Date of payment

Payments by the Agency are considered to have been carried out on the date when they are debited to its account.

21.11 Consequences of non-compliance

21.11.1 If the Agency does not pay within the payment deadlines (see above), the beneficiaries are entitled to late-payment interest at the rate applied by the European Central Bank (ECB) for its main refinancing operations in euros (‘reference rate’), plus three and a half points. The reference rate is the rate in force on the first day of the month in which the payment deadline expires, as published in the C series of the Official Journal of the European Union.

If the late-payment interest is lower than or equal to EUR 200, it will be paid to the coordinator only upon request submitted within two months of receiving the late payment.

Late-payment interest is not due if all beneficiaries are EU Member States (including regional and local government authorities or other public bodies acting on behalf of a Member State for the purpose of this Agreement).

38 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

Suspension of the payment deadline or payments (see Articles 47 and 48) will not be considered as late payment.

Late-payment interest covers the period running from the day following the due date for payment (see above), up to and including the date of payment.

Late-payment interest is not considered for the purposes of calculating the final grant amount.

21.11.2 If the coordinator breaches any of its obligations under this Article, the grant may be reduced (see Article 43) and the Agreement or the participation of the coordinator may be terminated (see Article 50).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 22 — CHECKS, REVIEWS, AUDITS AND INVESTIGATIONS — EXTENSION OF FINDINGS

22.1 Checks, reviews and audits by the Commission and the Agency

22.1.1 Right to carry out checks

The Commission or the Agency will — during the implementation of the action or afterwards — check the proper implementation of the action and compliance with the obligations under the Agreement, including assessing deliverables and reports.

For this purpose the Commission or the Agency may be assisted by external persons or bodies.

The Commission or the Agency may also request additional information in accordance with Article 17. The Commission or the Agency may request beneficiaries to provide such information to it directly.

Information provided must be accurate, precise and complete and in the format requested, including electronic format.

22.1.2 Right to carry out reviews

The Commission or the Agency may — during the implementation of the action or afterwards — carry out reviews on the proper implementation of the action (including assessment of deliverables and reports), compliance with the obligations under the Agreement and continued scientific or technological relevance of the action.

Reviews may be started up to two years after the payment of the balance. They will be formally notified to the coordinator or beneficiary concerned and will be considered to have started on the date of the formal notification.

If the review is carried out on a third party (see Articles 10 to 16), the beneficiary concerned must inform the third party.

The Commission or the Agency may carry out reviews directly (using its own staff) or indirectly (using external persons or bodies appointed to do so). It will inform the coordinator or beneficiary concerned of the identity of the external persons or bodies. They have the right to object to the appointment on grounds of commercial confidentiality.

39 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The coordinator or beneficiary concerned must provide — within the deadline requested — any information and data in addition to deliverables and reports already submitted (including information on the use of resources). The Commission or the Agency may request beneficiaries to provide such information to it directly.

The coordinator or beneficiary concerned may be requested to participate in meetings, including with external experts.

For on-the-spot reviews, the beneficiaries must allow access to their sites and premises, including to external persons or bodies, and must ensure that information requested is readily available.

Information provided must be accurate, precise and complete and in the format requested, including electronic format.

On the basis of the review findings, a ‘review report’ will be drawn up.

The Commission or the Agency will formally notify the review report to the coordinator or beneficiary concerned, which has 30 days to formally notify observations (‘contradictory review procedure’).

Reviews (including review reports) are in the language of the Agreement.

22.1.3 Right to carry out audits

The Commission or the Agency may — during the implementation of the action or afterwards — carry out audits on the proper implementation of the action and compliance with the obligations under the Agreement.

Audits may be started up to two years after the payment of the balance. They will be formally notified to the coordinator or beneficiary concerned and will be considered to have started on the date of the formal notification.

If the audit is carried out on a third party (see Articles 10 to 16), the beneficiary concerned must inform the third party.

The Commission or the Agency may carry out audits directly (using its own staff) or indirectly (using external persons or bodies appointed to do so). It will inform the coordinator or beneficiary concerned of the identity of the external persons or bodies. They have the right to object to the appointment on grounds of commercial confidentiality.

The coordinator or beneficiary concerned must provide — within the deadline requested — any information (including complete accounts, individual salary statements or other personal data) to verify compliance with the Agreement. The Commission or the Agency may request beneficiaries to provide such information to it directly.

For on-the-spot audits, the beneficiaries must allow access to their sites and premises, including to external persons or bodies, and must ensure that information requested is readily available.

Information provided must be accurate, precise and complete and in the format requested, including electronic format.

On the basis of the audit findings, a ‘draft audit report’ will be drawn up.

40 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The Commission or the Agency will formally notify the draft audit report to the coordinator or beneficiary concerned, which has 30 days to formally notify observations (‘contradictory audit procedure’). This period may be extended by the Commission or the Agency in justified cases.

The ‘final audit report’ will take into account observations by the coordinator or beneficiary concerned. The report will be formally notified to it.

Audits (including audit reports) are in the language of the Agreement.

The Commission or the Agency may also access the beneficiaries’ statutory records for the periodical assessment of unit costs or flat-rate amounts.

22.2 Investigations by the European Anti-Fraud Office (OLAF)

Under Regulations No 883/201315 and No 2185/9616 (and in accordance with their provisions and procedures), the European Anti-Fraud Office (OLAF) may — at any moment during implementation of the action or afterwards — carry out investigations, including on-the-spot checks and inspections, to establish whether, concerning the action funded under the Agreement, there has been fraud, corruption or any other illegal activity affecting the financial interests of the EU.

22.3 Checks and audits by the European Court of Auditors (ECA)

Under Article 287 of the Treaty on the Functioning of the European Union (TFEU) and Article 161 of the Financial Regulation No 966/201217, the European Court of Auditors (ECA) may — at any moment during implementation of the action or afterwards — carry out audits.

The ECA has the right of access for the purpose of checks and audits.

22.4 Checks, reviews, audits and investigations for international organisations not applicable

22.5 Consequences of findings in checks, reviews, audits and investigations —Extension of findings

22.5.1 Findings in this grant

Findings in checks, reviews, audits or investigations carried out in the context of this grant may lead to the rejection of ineligible costs (see Article 42), reduction of the grant (see Article 43), recovery of undue amounts (see Article 44) or to any of the other measures described in Chapter 6.

15 Regulation (EU, Euratom) No 883/2013 of the European Parliament and of the Council of 11 September 2013 concerning investigations conducted by the European Anti-Fraud Office (OLAF) and repealing Regulation (EC) No 1073/1999 of the European Parliament and of the Council and Council Regulation (Euratom) No 1074/1999 (OJ L 248, 18.09.2013, p. 1). 16 Council Regulation (Euratom, EC) No 2185/1996 of 11 November 1996 concerning on-the-spot checks and inspections carried out by the Commission in order to protect the European Communities' financial interests against fraud and other irregularities (OJ L 292, 15.11.1996, p. 2). 17 Regulation (EU, EURATOM) No 966/2012 of the European Parliament and of the Council of 25 October 2012 on the financial rules applicable to the general budget of the Union and repealing Council Regulation (EC, EURATOM)) No 1605/2002 (OJ L 298, 26.10.2012, p. 1).

41 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

Rejection of costs or reduction of the grant after the payment of the balance will lead to a revised final grant amount (see Article 5.4).

Findings in checks, reviews, audits or investigations may lead to a request for amendment for the modification of Annex 1 (see Article 55).

Checks, reviews, audits or investigations that find systemic or recurrent errors, irregularities, fraud or breach of obligations may also lead to consequences in other EU or Euratom grants awarded under similar conditions (‘extension of findings from this grant to other grants’).

Moreover, findings arising from an OLAF investigation may lead to criminal prosecution under national law.

22.5.2 Findings in other grants

The Commission or the Agency may extend findings from other grants to this grant (‘extension of findings from other grants to this grant’), if:

(a) the beneficiary concerned is found, in other EU or Euratom grants awarded under similar conditions, to have committed systemic or recurrent errors, irregularities, fraud or breach of obligations that have a material impact on this grant and

(b) those findings are formally notified to the beneficiary concerned — together with the list of grants affected by the findings — no later than two years after the payment of the balance of this grant.

The extension of findings may lead to the rejection of costs (see Article 42), reduction of the grant (see Article 43), recovery of undue amounts (see Article 44), suspension of payments (see Article 48), suspension of the action implementation (see Article 49) or termination (see Article 50).

22.5.3 Procedure

The Commission or the Agency will formally notify the beneficiary concerned the systemic or recurrent errors, together with the list of grants affected by the findings.

22.5.3.1 If the findings concern eligibility of costs: the formal notification will include:

(a) an invitation to submit observations on the list of grants affected by the findings;

(b) the request to submit revised financial statements for all grants affected;

(c) the correction rate for extrapolation established by the Commission or the Agency on the basis of the systemic or recurrent errors, to calculate the amounts to be rejected if the beneficiary concerned:

(i) considers that the submission of revised financial statements is not possible or practicable or

(ii) does not submit revised financial statements.

42 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The beneficiary concerned has 90 days from receiving notification to submit observations, revised financial statements or to propose a duly substantiated alternative correction method. This period may be extended by the Commission or the Agency in justified cases.

The Commission or the Agency will determine the amounts to be rejected on the basis of the revised financial statements, subject to their approval.

If the Commission or the Agency does not receive any observations or revised financial statements, does not accept the observations or the proposed alternative correction method or does not approve the revised financial statements, it will formally notify the beneficiary concerned the application of the initially notified correction rate for extrapolation.

If the Commission or the Agency accepts the alternative correction method proposed by the beneficiary concerned, it will formally notify the application of the accepted alternative correction method.

22.5.3.2 If the findings concern improper implementation or a breach of another obligation: the formal notification will include:

(a) an invitation to submit observations on the list of grants affected by the findings and

(b) the flat-rate the Commission or the Agency intends to apply according to the principle of proportionality.

The beneficiary concerned has 90 days from receiving notification to submit observations or to propose a duly substantiated alternative flat-rate.

If the Commission or the Agency does not receive any observations or does not accept the observations or the proposed alternative flat-rate, it will formally notify the beneficiary concerned the application of the initially notified flat-rate.

If the Commission or the Agency accepts the alternative flat-rate proposed by the beneficiary concerned, it will formally notify the application of the accepted alternative flat-rate.

22.6 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, any insufficiently substantiated costs will be ineligible (see Article 6) and will be rejected (see Article 42).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 23 — EVALUATION OF THE IMPACT OF THE ACTION

23.1 Right to evaluate the impact of the action

The Commission or the Agency may carry out interim and final evaluations of the impact of the action measured against the objective of the EU programme.

Evaluations may be started during implementation of the action and up to five years after the payment of the balance. The evaluation is considered to start on the date of the formal notification to the coordinator or beneficiaries.

43 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The Commission or the Agency may make these evaluations directly (using its own staff) or indirectly (using external bodies or persons it has authorised to do so).

The coordinator or beneficiaries must provide any information relevant to evaluate the impact of the action, including information in electronic format.

23.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the Commission or the Agency may apply the measures described in Chapter 6.

SECTION 3 RIGHTS AND OBLIGATIONS RELATED TO BACKGROUND AND RESULTS

SUBSECTION 1 GENERAL

ARTICLE 23a — MANAGEMENT OF INTELLECTUAL PROPERTY

23a.1 Obligation to take measures to implement the Commission Recommendation on the management of intellectual property in knowledge transfer activities

Beneficiaries that are universities or other public research organisations must take measures to implement the principles set out in Points 1 and 2 of the Code of Practice annexed to the Commission Recommendation on the management of intellectual property in knowledge transfer activities18.

This does not change the obligations set out in Subsections 2 and 3 of this Section.

The beneficiaries must ensure that researchers and third parties involved in the action are aware of them.

23a.2 Consequences of non-compliance

If a beneficiary breaches its obligations under this Article, the Agency may apply any of the measures described in Chapter 6.

SUBSECTION 2 RIGHTS AND OBLIGATIONS RELATED TO BACKGROUND

ARTICLE 24 — AGREEMENT ON BACKGROUND

The beneficiaries must identify and agree (in writing) on the background for the action (‘agreement on background’).

‘Background’ means any data, know-how or information — whatever its form or nature (tangible or intangible), including any rights such as intellectual property rights — that:

(a) is held by the beneficiaries before they acceded to the Agreement, and

18 Commission Recommendation C (2008) 1329 of 10.4.2008 on the management of intellectual property in knowledge transfer activities and the Code of Practice for universities and other public research institutions attached to this recommendation.

44 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

(b) is needed to implement the action or exploit the results.

ARTICLE 25 — ACCESS RIGHTS TO BACKGROUND

25.1 Exercise of access rights — Waiving of access rights — No sub-licensing

To exercise access rights, this must first be requested in writing (‘request for access’).

‘Access rights’ means rights to use results or background under the terms and conditions laid down in this Agreement.

Waivers of access rights are not valid unless in writing.

Unless agreed otherwise, access rights do not include the right to sub-license.

25.2 Access rights for other beneficiaries, for implementing their own tasks under the action

The beneficiaries must give each other access — on a royalty-free basis — to background needed to implement their own tasks under the action, unless the beneficiary that holds the background has — before acceding to the Agreement —:

(a) informed the other beneficiaries that access to its background is subject to legal restrictions or limits, including those imposed by the rights of third parties (including personnel), or

(b) agreed with the other beneficiaries that access would not be on a royalty-free basis.

25.3 Access rights for other beneficiaries, for exploiting their own results

The beneficiaries must give each other access — under fair and reasonable conditions — to background needed for exploiting their own results, unless the beneficiary that holds the background has — before acceding to the Agreement — informed the other beneficiaries that access to its background is subject to legal restrictions or limits, including those imposed by the rights of third parties (including personnel).

‘Fair and reasonable conditions’ means appropriate conditions, including possible financial terms or royalty-free conditions, taking into account the specific circumstances of the request for access, for example the actual or potential value of the results or background to which access is requested and/or the scope, duration or other characteristics of the exploitation envisaged.

Requests for access may be made — unless agreed otherwise — up to one year after the period set out in Article 3.

25.4 Access rights for affiliated entities

Unless otherwise agreed in the consortium agreement, access to background must also be given — under fair and reasonable conditions (see above; Article 25.3) and unless it is subject to legal restrictions or limits, including those imposed by the rights of third parties (including personnel) —

45 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2 to affiliated entities19 established in an EU Member State or ‘associated country’ 20, if this is needed to exploit the results generated by the beneficiaries to which they are affiliated.

Unless agreed otherwise (see above; Article 25.1), the affiliated entity concerned must make the request directly to the beneficiary that holds the background.

Requests for access may be made — unless agreed otherwise — up to one year after the period set out in Article 3.

25.5 Access rights for third parties not applicable

25.6 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

SUBSECTION 3 RIGHTS AND OBLIGATIONS RELATED TO RESULTS

ARTICLE 26 — OWNERSHIP OF RESULTS

26.1 Ownership by the beneficiary that generates the results

Results are owned by the beneficiary that generates them.

‘Results’ means any (tangible or intangible) output of the action such as data, knowledge or information — whatever its form or nature, whether it can be protected or not — that is generated in the action, as well as any rights attached to it, including intellectual property rights.

26.2 Joint ownership by several beneficiaries

Two or more beneficiaries own results jointly if:

(a) they have jointly generated them and

(b) it is not possible to:

(i) establish the respective contribution of each beneficiary, or

(ii) separate them for the purpose of applying for, obtaining or maintaining their protection (see Article 27).

19 For the definition, see ‘affiliated entity’ footnote (Article 14.1). 20 For the definition, see Article 2.1(3) of the Rules for Participation Regulation No 1290/2013: ‘associated country’ means a third country which is party to an international agreement with the Union, as identified in Article 7 of Horizon 2020 Framework Programme Regulation No 1291/2013. Article 7 sets out the conditions for association of non-EU countries to Horizon 2020.

46 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

The joint owners must agree (in writing) on the allocation and terms of exercise of their joint ownership (‘joint ownership agreement’), to ensure compliance with their obligations under this Agreement.

Unless otherwise agreed in the joint ownership agreement, each joint owner may grant non-exclusive licences to third parties to exploit jointly-owned results (without any right to sub-license), if the other joint owners are given:

(a) at least 45 days advance notice and

(b) fair and reasonable compensation.

Once the results have been generated, joint owners may agree (in writing) to apply another regime than joint ownership (such as, for instance, transfer to a single owner (see Article 30) with access rights for the others).

26.3 Rights of third parties (including personnel)

If third parties (including personnel) may claim rights to the results, the beneficiary concerned must ensure that it complies with its obligations under the Agreement.

If a third party generates results, the beneficiary concerned must obtain all necessary rights (transfer, licences or other) from the third party, in order to be able to respect its obligations as if those results were generated by the beneficiary itself.

If obtaining the rights is impossible, the beneficiary must refrain from using the third party to generate the results.

26.4 Agency ownership, to protect results

26.4.1 The Agency may — with the consent of the beneficiary concerned — assume ownership of results to protect them, if a beneficiary intends — up to four years after the period set out in Article 3 — to disseminate its results without protecting them, except in any of the following cases:

(a) the lack of protection is because protecting the results is not possible, reasonable or justified (given the circumstances);

(b) the lack of protection is because there is a lack of potential for commercial or industrial exploitation, or

(c) the beneficiary intends to transfer the results to another beneficiary or third party established in an EU Member State or associated country, which will protect them.

Before the results are disseminated and unless any of the cases above under Points (a), (b) or (c) applies, the beneficiary must formally notify the Agency and at the same time inform it of any reasons for refusing consent. The beneficiary may refuse consent only if it can show that its legitimate interests would suffer significant harm.

If the Agency decides to assume ownership, it will formally notify the beneficiary concerned within 45 days of receiving notification.

No dissemination relating to these results may before the end of this period or, if the Agency takes a positive decision, until it has taken the necessary steps to protect the results.

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26.4.2 The Agency may — with the consent of the beneficiary concerned — assume ownership of results to protect them, if a beneficiary intends — up to four years after the period set out in Article 3 — to stop protecting them or not to seek an extension of protection, except in any of the following cases:

(a) the protection is stopped because of a lack of potential for commercial or industrial exploitation;

(b) an extension would not be justified given the circumstances.

A beneficiary that intends to stop protecting results or not seek an extension must — unless any of the cases above under Points (a) or (b) applies — formally notify the Agency at least 60 days before the protection lapses or its extension is no longer possible and at the same time inform it of any reasons for refusing consent. The beneficiary may refuse consent only if it can show that its legitimate interests would suffer significant harm.

If the Agency decides to assume ownership, it will formally notify the beneficiary concerned within 45 days of receiving notification.

26.5 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to the any of the other measures described in Chapter 6.

ARTICLE 27 — PROTECTION OF RESULTS — VISIBILITY OF EU FUNDING

27.1 General obligation to protect the results

Each beneficiary must examine the possibility of protecting its results and must adequately protect them — for an appropriate period and with appropriate territorial coverage — if:

(a) the results can reasonably be expected to be commercially or industrially exploited and

(b) protecting them is possible, reasonable and justified (given the circumstances).

When deciding on protection, the beneficiary must consider its own legitimate interests and the legitimate interests (especially commercial) of the other beneficiaries.

27.2 Agency ownership, to protect the results

If a beneficiary intends not to protect its results, to stop protecting them or not seek an extension of protection, The Agency may — under certain conditions (see Article 26.4) — assume ownership to ensure their (continued) protection.

27.3 Information on EU funding

Applications for protection of results (including patent applications) filed by or on behalf of a beneficiary must — unless the Agency requests or agrees otherwise or unless it is impossible — include the following:

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“The project leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 634476”.

27.4 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such a breach may also lead to any of the other measures described in Chapter 6.

ARTICLE 28 — EXPLOITATION OF RESULTS

28.1 General obligation to exploit the results

Each beneficiary must — up to four years after the period set out in Article 3 — take measures aiming to ensure ‘exploitation’ of its results (either directly or indirectly, in particular through transfer or licensing; see Article 30) by:

(a) using them in further research activities (outside the action);

(b) developing, creating or marketing a product or process;

(d) using them in standardisation activities.

This does not change the security obligations in Article 37, which still apply.

28.2 Results that could contribute to European or international standards — Information on EU funding

If results are incorporated in a standard, the beneficiary concerned must — unless the Agency requests or agrees otherwise or unless it is impossible — ask the standardisation body to include the following statement in (information related to) the standard:

“Results incorporated in this standard received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 634476”.

28.3 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced in accordance with Article 43.

Such a breach may also lead to any of the other measures described in Chapter 6.

ARTICLE 29 — DISSEMINATION OF RESULTS — OPEN ACCESS — VISIBILITY OF EU FUNDING

29.1 General obligation to disseminate results

Unless it goes against their legitimate interests, each beneficiary must — as soon as possible — ‘disseminate’ its results by disclosing them to the public by appropriate means (other than those

49 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2 resulting from protecting or exploiting the results), including in scientific publications (in any medium).

This does not change the obligation to protect results in Article 27, the confidentiality obligations in Article 36, the security obligations in Article 37 or the obligations to protect personal data in Article 39, all of which still apply.

A beneficiary that intends to disseminate its results must give advance notice to the other beneficiaries of — unless agreed otherwise — at least 45 days, together with sufficient information on the results it will disseminate.

Any other beneficiary may object within — unless agreed otherwise — 30 days of receiving notification, if it can show that its legitimate interests in relation to the results or background would be significantly harmed. In such cases, the dissemination may not take place unless appropriate steps are taken to safeguard these legitimate interests.

If a beneficiary intends not to protect its results, it may — under certain conditions (see Article 26.4.1) — need to formally notify the Agency before dissemination takes place.

29.2 Open access to scientific publications

Each beneficiary must ensure open access (free of charge online access for any user) to all peer- reviewed scientific publications relating to its results.

In particular, it must:

(a) as soon as possible and at the latest on publication, deposit a machine-readable electronic copy of the published version or final peer-reviewed manuscript accepted for publication in a repository for scientific publications;

Moreover, the beneficiary must aim to deposit at the same time the research data needed to validate the results presented in the deposited scientific publications.

(b) ensure open access to the deposited publication — via the repository — at the latest:

(i) on publication, if an electronic version is available for free via the publisher, or

(ii) within six months of publication (twelve months for publications in the social sciences and humanities) in any other case.

The bibliographic metadata must be in a standard format and must include all of the following:

- the terms "European Union (EU)" and "Horizon 2020";

- the name of the action, acronym and grant number;

- the publication date, and length of embargo period if applicable, and

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- a persistent identifier.

29.3 Open access to research data not applicable

29.4 Information on EU funding — Obligation and right to use the EU emblem

Unless the Agency requests or agrees otherwise or unless it is impossible, any dissemination of results (in any form, including electronic) must:

(a) display the EU emblem and

(b) include the following text:

“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 634476”.

When displayed together with another logo, the EU emblem must have appropriate prominence.

For the purposes of their obligations under this Article, the beneficiaries may use the EU emblem without first obtaining approval from the Agency.

This does not however give them the right to exclusive use.

Moreover, they may not appropriate the EU emblem or any similar trademark or logo, either by registration or by any other means.

29.5 Disclaimer excluding Agency responsibility

Any dissemination of results must indicate that it reflects only the author's view and that the Agency is not responsible for any use that may be made of the information it contains.

29.6 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such a breach may also lead to any of the other measures described in Chapter 6.

ARTICLE 30 — TRANSFER AND LICENSING OF RESULTS

30.1 Transfer of ownership

Each beneficiary may transfer ownership of its results.

It must however ensure that its obligations under Articles 26.2, 26.4, 27, 28, 29, 30 and 31 also apply to the new owner and that this owner has the obligation to pass them on in any subsequent transfer.

This does not change the security obligations in Article 37, which still apply.

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Unless agreed otherwise (in writing) for specifically-identified third parties or unless impossible under applicable EU and national laws on mergers and acquisitions, a beneficiary that intends to transfer ownership of results must give at least 45 days advance notice to the other beneficiaries that still have (or still may request) access rights to the results. This notification must include sufficient information on the new owner to enable any beneficiary concerned to assess the effects on its access rights.

Unless agreed otherwise (in writing), any other beneficiary may object within 30 days of receiving notification, if it can show that the transfer would adversely affect its access rights. In this case, the transfer may not take place until agreement has been reached between the beneficiaries concerned.

30.2 Granting licenses

Each beneficiary may grant licences to its results (or otherwise give the right to exploit them), if:

(a) this does not impede the rights under Article 31 and

(b) not applicable.

In addition to Points (a) and (b), exclusive licences for results may be granted only if all the other beneficiaries concerned have waived their access rights (see Article 31.1).

This does not change the dissemination obligations in Article 29 or security obligations in Article 37, which still apply.

30.3 Agency right to object to transfers or licensing

The Agency may — up to four years after the period set out in Article 3 — object to a transfer of ownership or the exclusive licensing of results, if:

(a) it is to a third party established in a non-EU country not associated with Horizon 2020 and

(b) the Agency considers that the transfer or licence is not in line with EU interests regarding competitiveness or is inconsistent with ethical principles or security considerations.

A beneficiary that intends to transfer ownership or grant an exclusive licence must formally notify the Agency before the intended transfer or licensing takes place and:

- identify the specific results concerned;

- describe in detail the new owner or licensee and the planned or potential exploitation of the results, and

- include a reasoned assessment of the likely impact of the transfer or licence on EU competitiveness and its consistency with ethical principles and security considerations.

The Agency may request additional information.

If the Agency decides to object to a transfer or exclusive licence, it must formally notify the beneficiary concerned within 60 days of receiving notification (or any additional information it has requested).

No transfer or licensing may take place in the following cases:

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- pending the Agency decision, within the period set out above;

- if the Agency objects;

- until the conditions are complied with, if the Agency objection comes with conditions.

30.4 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such a breach may also lead to any of the other measures described in Chapter 6.

ARTICLE 31 — ACCESS RIGHTS TO RESULTS

31.1 Exercise of access rights — Waiving of access rights — No sub-licensing

The conditions set out in Article 25.1 apply.

The obligations set out in this Article do not change the security obligations in Article 37, which still apply.

31.2 Access rights for other beneficiaries, for implementing their own tasks under the action

The beneficiaries must give each other access — on a royalty-free basis — to results needed for implementing their own tasks under the action.

31.3 Access rights for other beneficiaries, for exploiting their own results

The beneficiaries must give each other — under fair and reasonable conditions (see Article 25.3) — access to results needed for exploiting their own results.

Requests for access may be made — unless agreed otherwise — up to one year after the period set out in Article 3.

31.4 Access rights of affiliated entities

Unless agreed otherwise in the consortium agreement, access to results must also be given — under fair and reasonable conditions (Article 25.3) — to affiliated entities established in an EU Member State or associated country, if this is needed for those entities to exploit the results generated by the beneficiaries to which they are affiliated.

Unless agreed otherwise (see above; Article 31.1), the affiliated entity concerned must make any such request directly to the beneficiary that owns the results.

Requests for access may be made — unless agreed otherwise — up to one year after the period set out in Article 3.

31.5 Access rights for the EU institutions, bodies, offices or agencies and EU Member States

The beneficiaries must give access to their results — on a royalty-free basis — to EU institutions, bodies, offices or agencies, for developing, implementing or monitoring EU policies or programmes.

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Such access rights are limited to non-commercial and non-competitive use.

This does not change the right to use any material, document or information received from the beneficiaries for communication and publicising activities (see Article 38.2).

31.6 Access rights for third parties not applicable

31.7 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

SECTION 4 OTHER RIGHTS AND OBLIGATIONS

ARTICLE 32 — RECRUITMENT AND WORKING CONDITIONS FOR RESEARCHERS

32.1 Obligation to take measures to implement the European Charter for Researchers and Code of Conduct for the Recruitment of Researchers

The beneficiaries must take all measures to implement the principles set out in the Commission Recommendation on the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers22, in particular regarding:

- working conditions;

- transparent recruitment processes based on merit, and

- career development.

The beneficiaries must ensure that researchers and third parties involved in the action are aware of them.

32.2 Consequences of non-compliance

If a beneficiary breaches its obligations under this Article, the Agency may apply any of the measures described in Chapter 6.

ARTICLE 33 — GENDER EQUALITY

33.1 Obligation to aim for gender equality

The beneficiaries must take all measures to promote equal opportunities between men and women in the implementation of the action. They must aim, to the extent possible, for a gender balance at all levels of personnel assigned to the action, including at supervisory and managerial level.

22 Commission recommendation (EC) No 251/2005 of 11 March 2005 on the European Charter for Researchers and on a Code of Conduct for the Recruitment of Researchers (OJ L 75, 22.03.2005, p. 67).

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33.2 Consequences of non-compliance

If a beneficiary breaches its obligations under this Article, the Agency may apply any of the measures described in Chapter 6.

ARTICLE 34 — ETHICS

34.1 General obligation to comply with ethical principles

The beneficiaries must carry out the action in compliance with:

(a) ethical principles (including the highest standards of research integrity — as set out, for instance, in the European Code of Conduct for Research Integrity23 — and including, in particular, avoiding fabrication, falsification, plagiarism or other research misconduct) and

(b) applicable international, EU and national law.

Funding will not be granted for activities carried out outside the EU if they are prohibited in all Member States.

The beneficiaries must ensure that the activities under the action have an exclusive focus on civil applications.

The beneficiaries must ensure that the activities under the action do not:

(a) aim at human cloning for reproductive purposes;

(b) intend to modify the genetic heritage of human beings which could make such changes heritable (with the exception of research relating to cancer treatment of the gonads, which may be financed), or

(c) intend to create human embryos solely for the purpose of research or for the purpose of stem cell procurement, including by means of somatic cell nuclear transfer.

34.2 Activities raising ethical issues

Activities raising ethical issues must comply with the ‘ethics requirements’ set out in Annex 1.

Before the beginning of an activity raising an ethical issue, the coordinator must submit (see Article 52) to the Agency copy of:

(a) any ethics committee opinion required under national law and

(b) any notification or authorisation for activities raising ethical issues required under national law.

If these documents are not in English, the coordinator must also submit an English summary of the submitted opinions, notifications and authorisations (containing, if available, the conclusions of the committee or authority concerned).

23 The European Code of Conduct for Research Integrity of ALLEA (All European Academies) and ESF (European Science Foundation) of March 2011. http://www.esf.org/fileadmin/Public_documents/Publications/Code_Conduct_ResearchIntegrity.pdf

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If these documents are specifically requested for the action, the request must contain an explicit reference to the action title. The coordinator must submit a declaration by each beneficiary concerned that all the submitted documents cover the action tasks.

34.3 Activities involving human embryos or human embryonic stem cells not applicable

34.4 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43) and the Agreement or participation of the beneficiary may be terminated (see Article 50).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 35 — CONFLICT OF INTERESTS

35.1 Obligation to avoid a conflict of interests

The beneficiaries must take all measures to prevent any situation where the impartial and objective implementation of the action is compromised for reasons involving economic interest, political or national affinity, family or emotional ties or any other shared interest (‘conflict of interests’).

They must formally notify to the Agency without delay any situation constituting or likely to lead to a conflict of interests and immediately take all the necessary steps to rectify this situation.

The Agency may verify that the measures taken are appropriate and may require additional measures to be taken by a specified deadline.

35.2 Consequences of non-compliance

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 36 — CONFIDENTIALITY

36.1 General obligation to maintain confidentiality

During implementation of the action and for four years after the period set out in Article 3, the parties must keep confidential any data, documents or other material (in any form) that is identified as confidential at the time it is disclosed (‘confidential information’).

If a beneficiary requests, the Agency may agree to keep such information confidential for an additional period beyond the initial four years.

If information has been identified as confidential only orally, it will be considered to be confidential only if this is confirmed in writing within 15 days of the oral disclosure.

Unless otherwise agreed between the parties, they may use confidential information only to implement the Agreement.

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The beneficiaries may disclose confidential information to their personnel or third parties involved in the action only if they:

(a) need to know to implement the Agreement and

(b) are bound by an obligation of confidentiality.

This does not change the security obligations in Article 37, which still apply.

The Agency may disclose confidential information to its staff, other EU institutions and bodies or third parties, if:

(a) this is necessary to implement the Agreement or safeguard the EU’s financial interests and

(b) the recipients of the information are bound by an obligation of confidentiality.

Under the conditions set out in Article 4 of the Rules for participation Regulation No 1290/201324, the Commission must moreover make available information on the results to other EU institutions, bodies, offices or agencies as well as Member States or associated countries.

The confidentiality obligations no longer apply if:

(a) the disclosing party agrees to release the other party;

(b) the information was already known by the recipient or is given to him without obligation of confidentiality by a third party that was not bound by any obligation of confidentiality;

(d) the information becomes generally and publicly available, without breaching any confidentiality obligation, or

(e) the disclosure of the information is required by EU or national law.

36.2 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 37 — SECURITY-RELATED OBLIGATIONS

37.1 Activities raising security issues not applicable

24 Regulation (EU) No 1290/2013 of the European Parliament and of the Council of 11 December 2013 laying down the rules for participation and dissemination in "Horizon 2020 - the Framework Programme for Research and Innovation (2014-2020)" (OJ L 347, 20.12.2013 p.81).

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37.2 Classified deliverables not applicable

37.3 Activities involving dual-use goods or dangerous materials and substances not applicable

37.4 Consequences of non-compliance not applicable

ARTICLE 38 — PROMOTING THE ACTION — VISIBILITY OF EU FUNDING

38.1 Communication activities by beneficiaries

38.1.1 General obligation to promote the action and its results

The beneficiaries must promote the action and its results, by providing targeted information to multiple audiences (including the media and the public) in a strategic and effective manner.

This does not change the dissemination obligations in Article 29, the confidentiality obligations in Article 36 or the security obligations in Article 37, all of which still apply.

Before engaging in a communication activity expected to have a major media impact, the beneficiaries must inform the Agency (see Article 52).

38.1.2 Information on EU funding — Obligation and right to use the EU emblem

Unless the Agency requests or agrees otherwise or unless it is impossible, any communication activity related to the action (including in electronic form, via social media, etc.) and any infrastructure funded by the grant must:

(a) display the EU emblem and

(b) include the following text:

“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 634476”.

When displayed together with another logo, the EU emblem must have appropriate prominence.

For the purposes of their obligations under this Article, the beneficiaries may use the EU emblem without first obtaining approval from the Agency.

This does not, however, give them the right to exclusive use.

Moreover, they may not appropriate the EU emblem or any similar trademark or logo, either by registration or by any other means.

38.1.3 Disclaimer excluding Agency responsibility

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Any communication activity related to the action must indicate that it reflects only the author's view and that the Agency is not responsible for any use that may be made of the information it contains.

38.2 Communication activities by the Agency

38.2.1 Right to use beneficiaries’ materials, documents or information

The Agency may use, for its communication and publicising activities, information relating to the action, documents notably summaries for publication and public deliverables as well as any other material, such as pictures or audio-visual material that it receives from any beneficiary (including in electronic form).

This does not change the confidentiality obligations in Article 36 and the security obligations in Article 37, all of which still apply.

However, if the Agency’s use of these materials, documents or information would risk compromising legitimate interests, the beneficiary concerned may request the Agency not to use it (see Article 52).

The right to use a beneficiary’s materials, documents and information includes:

(a) use for its own purposes (in particular, making them available to persons working for the Agency or any other EU institution, body, office or agency or body or institutions in EU Member States; and copying or reproducing them in whole or in part, in unlimited numbers);

(b) distribution to the public (in particular, publication as hard copies and in electronic or digital format, publication on the internet, as a downloadable or non-downloadable file, broadcasting by any channel, public display or presentation, communicating through press information services, or inclusion in widely accessible databases or indexes);

(c) editing or redrafting for communication and publicising activities (including shortening, summarising, inserting other elements (such as meta-data, legends, other graphic, visual, audio or text elements), extracting parts (e.g. audio or video files), dividing into parts, use in a compilation);

(d) translation;

(e) giving access in response to individual requests under Regulation No 1049/200125, without the right to reproduce or exploit;

(f) storage in paper, electronic or other form;

(g) archiving, in line with applicable document-management rules, and

(h) the right to authorise third parties to act on its behalf or sub-license the modes of use set out in Points (b),(c),(d) and (f) to third parties if needed for the communication and publicising activities of the Agency.

25 Regulation (EC) No 1049/2001 of the European Parliament and of the Council of 30 May 2001 regarding public access to European Parliament, Council and Commission documents, OJ L 145, 31.5.2001, p. 43.

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If the right of use is subject to rights of a third party (including personnel of the beneficiary), the beneficiary must ensure that it complies with its obligations under this Agreement (in particular, by obtaining the necessary approval from the third parties concerned).

Where applicable (and if provided by the beneficiaries), the Agency will insert the following information:

38.3 Consequences of non-compliance

If a beneficiary breaches any of its obligations under this Article, the grant may be reduced (see Article 43).

Such breaches may also lead to any of the other measures described in Chapter 6.

ARTICLE 39 — PROCESSING OF PERSONAL DATA

39.1 Processing of personal data by the Agency

Any personal data under the Agreement will be processed by the Agency under Regulation No 45/200126 and according to the ‘notifications of the processing operations’ to the Data Protection Officer (DPO) of the Agency (publicly accessible in the DPO register).

Such data will be processed by the ‘data controller’ of the Agency for the purposes of implementing, managing and monitoring the Agreement (including checks, reviews, audits and investigations; see Article 22).

The persons whose personal data are processed have the right to access and correct their own personal data. For this purpose, they must send any queries about the processing of their personal data to the data controller, via the contact point indicated in the ‘service specific privacy statement (SSPS)’ on the Agency's websites.

They also have the right to have recourse at any time to the European Data Protection Supervisor (EDPS).

39.2 Processing of personal data by the beneficiaries

The beneficiaries must process personal data under the Agreement in compliance with applicable EU and national law on data protection (including authorisations or notification requirements).

The beneficiaries may grant their personnel access only to data that is strictly necessary for implementing, managing and monitoring the Agreement.

The beneficiaries must inform the personnel whose personal data are collected and processed by the Agency. For this purpose, they must provide them with the service specific privacy statement (SSPS) (see above), before transmitting their data to the Agency.

26 Regulation (EC) No 45/2001 of the European Parliament and of the Council of 18 December 2000 on the protection of individuals with regard to the processing of personal data by the Community institutions and bodies and on the free movement of such data (OJ L 8, 12.01.2001, p. 1).

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39.3 Consequences of non-compliance

If a beneficiary breaches any of its obligations under Article 39.2, the Agency may apply any of the measures described in Chapter 6.

ARTICLE 40 — ASSIGNMENTS OF CLAIMS FOR PAYMENT AGAINST THE AGENCY

The beneficiaries may not assign any of their claims for payment against the Agency to any third party, except if approved by the Agency on the basis of a reasoned, written request by the coordinator (on behalf of the beneficiary concerned).

If the Agency has not accepted the assignment or the terms of it are not observed, the assignment will have no effect on it.

In no circumstances will an assignment release the beneficiaries from their obligations towards the Agency.

CHAPTER 5 DIVISION OF BENEFICIARIES’ ROLES AND RESPONSIBILITIES

ARTICLE 41 — DIVISION OF BENEFICIARIES’ ROLES AND RESPONSIBILITIES

41.1 Roles and responsibilities towards the Agency

The beneficiaries have full responsibility for implementing the action and complying with the Agreement.

The beneficiaries are jointly and severally liable for the technical implementation of the action as described in Annex 1. If a beneficiary fails to implement its part of the action, the other beneficiaries become responsible for implementing this part (without being entitled to any additional EU funding for doing so), unless the Agency expressly relieves them of this obligation.

The financial responsibility of each beneficiary is governed by Articles 44, 45 and 46.

41.2 Internal division of roles and responsibilities

The internal roles and responsibilities of the beneficiaries are divided as follows:

(a) Each beneficiary must:

(i) keep information stored in the Beneficiary Register (in the electronic exchange system) up to date (see Article 17);

(ii) inform the coordinator immediately of any events or circumstances likely to affect significantly or delay the implementation of the action (see Article 17);

(iii) submit to the coordinator in good time:

- individual financial statements for itself and its linked third parties and, if required, certificates on the financial statements (see Article 20);

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- the data needed to draw up the technical reports (see Article 20);

- ethics committee opinions and notifications or authorisations for activities raising ethical issues (see Article 34);

- any other documents or information required by the Agency under the Agreement, unless the Agreement requires the beneficiary to submit this information directly to the Agency.

(b) The coordinator must:

(i) monitor that the action is implemented properly (see Article 7);

(ii) act as the intermediary for all communications between the beneficiaries and the Agency (in particular, providing the Agency with the information described in Article 17), unless the Agreement specifies otherwise;

(iii) request and review any documents or information required by the Agency and verify their completeness and correctness before passing them on to the Agency;

(iv) submit the deliverables and reports to the Agency (see Articles 19 and 20);

(v) ensure that all payments are made to the other beneficiaries without unjustified delay (see Article 21);

(vi) inform the Agency of the amounts paid to each beneficiary, when required under the Agreement (see Articles 44 and 50) or requested by the Agency.

The coordinator may not delegate the above-mentioned tasks to any other beneficiary or subcontract them to any third party.

41.3 Internal arrangements between beneficiaries — Consortium agreement

The beneficiaries must have internal arrangements regarding their operation and co-ordination to ensure that the action is implemented properly. These internal arrangements must be set out in a written ‘consortium agreement’ between the beneficiaries, which may cover:

- internal organisation of the consortium;

- management of access to the electronic exchange system;

- distribution of EU funding;

- additional rules on rights and obligations related to background and results (including whether access rights remain or not, if a beneficiary is in breach of its obligations) (see Section 3);

- settlement of internal disputes;

- liability, indemnification and confidentiality arrangements between the beneficiaries.

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The consortium agreement must not contain any provision contrary to the Agreement.

41.4 Relationship with complementary beneficiaries — Collaboration agreement not applicable

41.5 Relationship with partners of a joint action — Coordination agreement not applicable

CHAPTER 6 REJECTION OF COSTS — REDUCTION OF THE GRANT — RECOVERY — PENALTIES — DAMAGES — SUSPENSION — TERMINATION — FORCE MAJEURE

SECTION 1 REJECTION OF COSTS — REDUCTION OF THE GRANT — RECOVERY — PENALTIES

ARTICLE 42 — REJECTION OF INELIGIBLE COSTS

42.1 Conditions

42.1.1 The Agency will — at the time of an interim payment, at the payment of the balance or afterwards — reject any costs which are ineligible (see Article 6), in particular following checks, reviews, audits or investigations (see Article 22).

42.1.2 The rejection may also be based on the extension of findings from other grants to this grant, under the conditions set out in Article 22.5.2.

42.2 Ineligible costs to be rejected — Calculation — Procedure

Ineligible costs will be rejected in full.

If the Agency rejects costs without reduction of the grant (see Article 43) or recovery of undue amounts (see Article 44), it will formally notify the coordinator or beneficiary concerned the rejection of costs, the amounts and the reasons why (if applicable, together with the notification of amounts due; see Article 21.5). The coordinator or beneficiary concerned may — within 30 days of receiving notification — formally notify the Agency of its disagreement and the reasons why.

If the Agency rejects costs with reduction of the grant or recovery of undue amounts , it will formally notify the rejection in the ‘pre-information letter’ on reduction or recovery set out in Articles 43 and 44.

42.3 Effects

If the Agency rejects costs at the time of an interim payment or the payment of the balance, it will deduct them from the total eligible costs declared, for the action, in the periodic or final summary financial statement as set out in Articles 21.3 or 21.4 statement (see Articles 20.3 and 20.4). It will then calculate the interim payment or payment of the balance.

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If the Agency — after an interim payment but before the payment of the balance — rejects costs declared in a periodic summary financial statement, it will deduct them from the total eligible costs declared, for the action, in the next periodic summary financial statement or in the final summary financial statement. It will then calculate the interim payment or payment of the balance as set out in Articles 21.3 or 21.4.

If the Agency rejects costs after the payment of the balance, it will deduct the amount rejected from the total eligible costs declared, by the beneficiary, in the final summary financial statement. It will then calculate the revised final grant amount as set out in Article 5.4.

ARTICLE 43 — REDUCTION OF THE GRANT

43.1 Conditions

43.1.1 The Agency may — at the payment of the balance or afterwards — reduce the maximum grant amount (see Article 5.1), if the action has not been implemented properly as described in Annex 1 or another obligation under the Agreement has been breached.

43.1.2 The Agency may also reduce the maximum grant amount on the basis of the extension of findings from other grants to this grant, under the conditions set out in Article 22.5.2.

43.2 Amount to be reduced — Calculation — Procedure

The amount of the reduction will be proportionate to the improper implementation of the action or to the seriousness of the breach.

Before reduction of the grant, the Agency will formally notify a ‘pre-information letter’ to the coordinator or beneficiary concerned:

- informing it of its intention to reduce the grant, the amount it intends to reduce and the reasons why and

- inviting it to submit observations within 30 days of receiving notification

If the Agency does not receive any observations or decides to pursue reduction despite the observations it has received, it will formally notify confirmation of the reduction (if applicable, together with the notification of amounts due; see Article 21).

43.3 Effects

If the Agency reduces the grant at the time of the payment of the balance, it will calculate the reduced grant amount for the action and then determine the amount due as payment of the balance (see Articles 5.3.4 and 21.4).

If the Agency reduces the grant after the payment of the balance, it will calculate the revised final grant amount for the beneficiary concerned (see Article 5.4). If the revised final grant amount for the beneficiary concerned is lower than its share of the final grant amount, the Agency will recover the difference (see Article 44).

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ARTICLE 44 — RECOVERY OF UNDUE AMOUNTS

44.1 Amount to be recovered — Calculation — Procedure

The Agency will — after termination of the participation of a beneficiary, at the payment of the balance or afterwards — recover any amount that was paid but is not due under the Agreement.

Each beneficiary’s financial responsibility in case of recovery is limited to its own debt (including undue amounts paid by the Agency for costs declared by its linked third parties), except for the amount retained for the Guarantee Fund (see Article 21.4).

44.1.1 Recovery after termination of a beneficiary’s participation

If recovery takes place after termination of a beneficiary’s participation (including the coordinator), the Agency will recover the undue amount from the beneficiary concerned by formally notifying it a debit note (see Article 50.2 and 50.3). This note will specify the amount to be recovered, the terms and the date for payment.

If payment is not made by the date specified in the debit note, the Agency will recover the amount:

(a) by ‘offsetting’ it — without the beneficiary’s consent — against any amounts owed to the beneficiary concerned by the Commission or an executive agency (from the EU or Euratom budget). In exceptional circumstances, to safeguard the EU’s financial interests, the Agency may offset before the payment date specified in the debit note;

(b) not applicable, and/or

(c) by taking legal action or by adopting an enforceable decision under Article 299 of the Treaty on the Functioning of the EU (TFEU) (see Article 57).

If payment is not made by the date specified in the debit note, the amount to be recovered (see above) will be increased by late-payment interest at the rate set out in Article 21.11, from the day following the payment date in the debit note, up to and including the date the Agency receives full payment of the amount.

Partial payments will be first credited against expenses, charges and late-payment interest and then against the principal.

Bank charges incurred in the recovery process will be borne by the beneficiary, unless Directive 2007/64/EC27 applies.

44.1.2 Recovery at payment of the balance

If the payment of the balance takes the form of a recovery (see Article 21.4), the Agency will formally notify a ‘pre-information letter’ to the coordinator:

- informing it of its intention to recover, the amount due as the balance and the reasons why;

27 Directive 2007/64/EC of the European Parliament and of the Council of 13 November 2007 on payment services in the internal market amending Directives 97/7/EC, 2002/65/EC, 2005/60/EC and 2006/48/EC and repealing Directive 97/5/EC (OJ L 319, 05.12.2007, p. 1).

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- specifying that it intends to deduct the amount to be recovered from the amount retained for the Guarantee Fund;

- requesting the coordinator to submit a report on the distribution of payments to the beneficiaries within 30 days of receiving notification, and

- inviting the coordinator to submit observations within 30 days of receiving notification.

If no observations are submitted or the Agency decides to pursue recovery despite the observations it has received, it will confirm recovery (together with the notification of amounts due; see Article 21.5) and:

- pay the difference between the amount to be recovered and the amount retained for the Guarantee Fund, if the difference is positive or

- formally notify to the coordinator a debit note for the difference between the amount to be recovered and the amount retained for the Guarantee Fund, if the difference is negative. This note will also specify the terms and the date for payment.

If the coordinator does not repay the Agency by the date in the debit note and has not submitted the report on the distribution of payments: the Agency will recover the amount set out in the debit note from the coordinator (see below).

If the coordinator does not repay the Agency by the date in the debit note, but has submitted the report on the distribution of payments: the Agency will:

(a) identify the beneficiaries for which the amount calculated as follows is negative:

{{{{beneficiary’s costs declared in the final summary financial statement and approved by the Agency multiplied by the reimbursement rate set out in Article 5.2 for the beneficiary concerned

plus

its linked third parties’ costs declared in the final summary financial statement and approved by the Agency multiplied by the reimbursement rate set out in Article 5.2 for each linked third party concerned}

divided by

the EU contribution for the action calculated according to Article 5.3.1}

multiplied by

the final grant amount (see Article 5.3)},

minus

{pre-financing and interim payments received by the beneficiary}}.

(b) formally notify to each beneficiary identified according to point (a) a debit note specifying the terms and date for payment. The amount of the debit note is calculated as follows:

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{{amount calculated according to point (a) for the beneficiary concerned

divided by

the sum of the amounts calculated according to point (a) for all the beneficiaries identified according to point (a)}

multiplied by

the amount set out in the debit note formally notified to the coordinator}.

If payment is not made by the date specified in the debit note, the Agency will recover the amount:

(a) by offsetting it — without the beneficiary’s consent — against any amounts owed to the beneficiary concerned by the Commission or an executive agency (from the EU or Euratom budget).

In exceptional circumstances, to safeguard the the EU’s financial interests, the Agency may offset before the payment date specified in the debit note;

(b) by drawing on the Guarantee Fund. The Agency will formally notify the beneficiary concerned the debit note on behalf of the Guarantee Fund and recover the amount:

(i) not applicable,

(ii) by taking legal action or by adopting an enforceable decision under Article 299 of the Treaty on the Functioning of the EU (TFEU) (see Article 57).

If payment is not made by the date in the debit note, the amount to be recovered (see above) will be increased by late-payment interest at the rate set out in Article 21.11, from the day following the payment date in the debit note, up to and including the date the Agency receives full payment of the amount.

Partial payments will be first credited against expenses, charges and late-payment interest and then against the principal.

Bank charges incurred in the recovery process will be borne by the beneficiary, unless Directive 2007/64/EC applies.

44.1.3 Recovery of amounts after payment of the balance

If, for a beneficiary, the revised final grant amount (see Article 5.4) is lower than its share of the final grant amount, it must repay the difference to the Agency.

The beneficiary’s share of the final grant amount is calculated as follows:

{{{beneficiary’s costs declared in the final summary financial statement and approved by the Agency multiplied by the reimbursement rate set out in Article 5.2 for the beneficiary concerned plus

67 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2 its linked third parties’ costs declared in the final summary financial statement and approved by the Agency multiplied by the reimbursement rate set out in Article 5.2 for each linked third party concerned} divided by the EU contribution for the action calculated according to Article 5.3.1} multiplied by the final grant amount (see Article 5.3)}.

If the coordinator has not distributed amounts received (see Article 21.7), the Commission will also recover these amounts.

The Agency will formally notify a pre-information letter to the beneficiary concerned:

- informing it of its intention to recover, the due amount and the reasons why and

- inviting it to submit observations within 30 days of receiving notification.

If no observations are submitted or the Agency decides to pursue recovery despite the observations it has received, it will confirm the amount to be recovered and formally notify to the beneficiary concerned a debit note. This note will also specify the terms and the date for payment.

If payment is not made by the date specified in the debit note, the Agency will recover the amount:

(a) by offsetting it — without the beneficiary’s consent — against any amounts owed to the beneficiary concerned by the Commission or an executive agency (from the EU or Euratom budget).

In exceptional circumstances, to safeguard the EU’s financial interests, the Agency may offset before the payment date specified in the debit note;

(b) by drawing on the Guarantee Fund. The Agency will formally notify the beneficiary concerned the debit note on behalf of the Guarantee Fund and recover the amount:

(i) not applicable

(ii) by taking legal action or by adopting an enforceable decision under Article 299 of the Treaty on the Functioning of the EU (TFEU) (see Article 57).

If payment is not made by the date in the debit note, the amount to be recovered (see above) will be increased by late-payment interest at the rate set out in Article 21.11, from the day following the date for payment in the debit note, up to and including the date the Agency receives full payment of the amount.

Partial payments will be first credited against expenses, charges and late-payment interest and then against the principal.

Bank charges incurred in the recovery process will be borne by the beneficiary, unless Directive 2007/64/EC applies.

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ARTICLE 45 — ADMINISTRATIVE AND FINANCIAL PENALTIES

45.1 Conditions

Under Articles 109 and 131(4) of the Financial Regulation No 966/2012, the Agency may impose administrative and financial penalties if a beneficiary:

(a) has committed substantial errors, irregularities or fraud or is in serious breach of its obligations under the Agreement or

(b) has made false declarations about information required under the Agreement or for the submission of the proposal (or has not supplied such information).

Each beneficiary is responsible for paying the financial penalties imposed on it.

Under Article 109(3) of the Financial Regulation No 966/2012, the Agency may — under certain conditions and limits — publish decisions imposing administrative or financial penalties.

45.2 Duration — Amount of penalty — Calculation

Administrative penalties exclude the beneficiary from all contracts and grants financed from the EU or Euratom budget for a maximum of five years from the date the infringement is established by the Agency.

If the beneficiary commits another infringement within five years of the date the first infringement is established, the Agency may extend the exclusion period up to 10 years.

Financial penalties will be between 2% and 10% of the maximum EU contribution indicated, for the beneficiary concerned, in the estimated budget (see Annex 2).

If the beneficiary commits another infringement within five years of the date the first infringement is established, the Agency may increase the rate of financial penalties to between 4% and 20%.

45.3 Procedure

Before applying a penalty, the Agency will formally notify the beneficiary concerned:

- informing it of its intention to impose a penalty, its duration or amount and the reasons why and

- inviting it to submit observations within 30 days.

If the Agency does not receive any observations or decides to impose the penalty despite of observations it has received, it will formally notify confirmation of the penalty to the beneficiary concerned and — in case of financial penalties — deduct the penalty from the payment of the balance or formally notify a debit note, specifying the amount to be recovered, the terms and the date for payment.

If payment is not made by the date specified in the debit note, the Agency may recover the amount:

(a) by offsetting it — without the beneficiary’s consent — against any amounts owed to the beneficiary concerned by the Commission or an executive agency (from the EU or Euratom budget).

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In exceptional circumstances, to safeguard the EU’s financial interests, the Agency may offset before the payment date specified in the debit note;

(b) by taking legal action or by adopting an enforceable decision under Article 299 of the Treaty on the Functioning of the EU (TFEU) (see Article 57).

Partial payments will be first credited against expenses, charges and late-payment interest and then against the principal.

Bank charges incurred in the recovery process will be borne by the beneficiary, unless Directive 2007/64/EC applies.

SECTION 2 LIABILITY FOR DAMAGES

ARTICLE 46 — LIABILITY FOR DAMAGES

46.1 Liability of the Agency

The Agency cannot be held liable for any damage caused to the beneficiaries or to third parties as a consequence of implementing the Agreement, including for gross negligence.

The Agency cannot be held liable for any damage caused by any of the beneficiaries or third parties involved in the action, as a consequence on implementing the Agreement.

46.2 Liability of the beneficiaries

46.2.1 Conditions

Except in case of force majeure (see Article 51), the beneficiaries must compensate the Agency for any damage it sustains as a result of the implementation of the action or because the action was not implemented in full compliance with the Agreement.

Each beneficiary is responsible for paying the damages claimed from it.

46.2.2 Amount of damages - Calculation

The amount the Agency can claim from a beneficiary will correspond to the damage caused by that beneficiary.

46.2.3 Procedure

Before claiming damages, the Agency will formally notify the beneficiary concerned:

- informing it of its intention to claim damages, the amount and the reasons why and

- inviting it to submit observations within 30 days.

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If the Agency does not receive any observations or decides to claim damages despite the observations it has received, it will formally notify confirmation of the claim for damages and a debit note, specifying the amount to be recovered, the terms and the date for payment.

If payment is not made by the date specified in the debit note, the Agency may recover the amount:

(a) by offsetting it — without the beneficiary’s consent — against any amounts owed to the beneficiary concerned by the Commission or an executive agency (from the EU or Euratom budget).

In exceptional circumstances, to safeguard the EU’s financial interests, the Agency may offset before the payment date specified in the debit note;

(b) by taking legal action or by adopting an enforceable decision under Article 299 of the Treaty on the Functioning of the EU (TFEU) (see Article 57).

Partial payments will be first credited against expenses, charges and late-payment interest and then against the principal.

Bank charges incurred in the recovery process will be borne by the beneficiary, unless Directive 2007/64/EC applies.

SECTION 3 SUSPENSION AND TERMINATION

ARTICLE 47 — SUSPENSION OF PAYMENT DEADLINE

47.1 Conditions

The Agency may — at any moment — suspend the payment deadline (see Article 21.2 to 21.4) if a request for payment (see Article 20) cannot be approved because:

(a) it does not comply with the provisions of the Agreement (see Article 20);

(b) the technical reports or financial reports have not been submitted or are not complete or additional information is needed, or

(c) there is doubt about the eligibility of the costs declared in the financial statements and additional checks, reviews, audits or investigations are necessary.

47.2 Procedure

The Agency will formally notify the coordinator of the suspension and the reasons why.

The suspension will take effect the day notification is sent by the Agency (see Article 52).

If the conditions for suspending the payment deadline are no longer met, the suspension will be lifted — and the remaining period will resume.

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If the suspension exceeds two months, the coordinator may request the Agency if the suspension will continue.

If the payment deadline has been suspended due to the non-compliance of the technical or financial reports (see Article 20) and the revised report or statement is not submitted or was submitted but is also rejected, the Agency may also terminate the Agreement or the participation of the beneficiary (see Article 50.3.1(l)).

ARTICLE 48 — SUSPENSION OF PAYMENTS

48.1 Conditions

The Agency may — at any moment — suspend, in whole or in part, the pre-financing payment and interim payments for one or more beneficiaries or the payment of the balance for all beneficiaries, if a beneficiary:

(a) has committed or is suspected of having committed substantial errors, irregularities, fraud or serious breach of obligations in the award procedure or under this Agreement or

(b) has committed — in other EU or Euratom grants awarded to it under similar conditions — systemic or recurrent errors, irregularities, fraud or serious breach of obligations that have a material impact on this grant (extension of findings from other grants to this grant; see Article 22.5.2).

48.2 Procedure

Before suspending payments, the Agency will formally notify the coordinator:

- informing it of its intention to suspend payments and the reasons why and

- inviting it to submit observations within 30 days of receiving notification.

If the Agency does not receive observations or decides to pursue the procedure despite the observations it has received, it will formally notify confirmation of the suspension. Otherwise, it will formally notify that the suspension procedure is not continued.

The suspension will take effect the day the confirmation notification is sent by the Agency.

If the conditions for resuming payments are met, the suspension will be lifted. The Commission will formally notify the coordinator.

During the suspension, the periodic report(s) (see Article 20.3) must not contain any individual financial statements from the beneficiary concerned and its linked third parties. When the Agency resumes payments, the coordinator may include them in the next periodic report.

The beneficiaries may suspend implementation of the action (see Article 49.1) or terminate the Agreement or the participation of the beneficiary concerned (see Article 50.1 and 50.2).

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ARTICLE 49 — SUSPENSION OF THE ACTION IMPLEMENTATION

49.1 Suspension of the action implementation, by the beneficiaries

49.1.1 Conditions

The beneficiaries may suspend implementation of the action or any part of it, if exceptional circumstances — in particular force majeure (see Article 51) — make implementation impossible or excessively difficult.

49.1.2 Procedure

The coordinator must immediately formally notify to the Agency the suspension (see Article 52), stating:

- the reasons why and

- the expected date of resumption.

The suspension will take effect the day this notification is received by the Agency.

Once circumstances allow for implementation to resume, the coordinator must immediately formally notify the Agency and request an amendment of the Agreement to set the date on which the action will be resumed, extend the duration of the action and make other changes necessary to adapt the action to the new situation (see Article 55) — unless the Agreement or the participation of a beneficiary has been terminated (see Article 50).

The suspension will be lifted with effect from the resumption date set out in the amendment. This date may be before the date on which the amendment enters into force.

Costs incurred during suspension of the action implementation are not eligible (see Article 6).

49.2 Suspension of the action implementation, by the Agency

49.2.1 Conditions

The Agency may suspend implementation of the action or any part of it:

(a) if a beneficiary has committed or is suspected of having committed substantial errors, irregularities, fraud or serious breach of obligations in the award procedure or under this Agreement;

(b) if a beneficiary has committed — in other EU or Euratom grants awarded to it under similar conditions — systemic or recurrent errors, irregularities, fraud or serious breach of obligations that have a material impact on this grant (extension of findings from other grants to this grant; see Article 22.5.2), or

49.2.2 Procedure

Before suspending implementation of the action, the Agency will formally notify the coordinator:

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- informing it of its intention to suspend the implementation and the reasons why and

- inviting it to submit observations within 30 days of receiving notification.

The suspension will take effect five days after confirmation notification is received by the coordinator (or on a later date specified in the notification).

It will be lifted if the conditions for resuming implementation of the action are met.

The coordinator will be formally notified of the lifting and the Agreement will be amended to set the date on which the action will be resumed, extend the duration of the action and make other changes necessary to adapt the action to the new situation (see Article 55) — unless the Agreement has already been terminated (see Article 50).

The suspension will be lifted with effect from the resumption date set out in the amendment. This date may be before the date on which the amendment enters into force.

Costs incurred during suspension are not eligible (see Article 6).

The beneficiaries may not claim damages due to suspension by the Agency (see Article 46).

Suspension of the action implementation does not affect the Agency's right to terminate the Agreement or participation of a beneficiary (see Article 50), reduce the grant or recover amounts unduly paid (see Articles 43 and 44).

ARTICLE 50 — TERMINATION OF THE AGREEMENT OR OF PARTICIPATION FOR ONE OR MORE BENEFICIARIES

50.1 Termination of the Agreement by the beneficiaries

50.1.1 Conditions and procedure

The beneficiaries may terminate the Agreement.

The coordinator must formally notify termination to the Agency (see Article 52), stating:

- the reasons why and

- the date the termination will take effect. This date must be after the notification.

If no reasons are given or if the Agency considers the reasons do not justify termination, the Agreement will be considered to have been ‘terminated improperly’.

The termination will take effect on the day specified in the notification.

50.1.2 Effects

The coordinator must — within 60 days from when termination takes effect — submit:

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(i) a periodic report (for the open reporting period until termination; see Article 20.3) and

(ii) the final report (see Article 20.4).

If the Agency does not receive the reports within the deadline (see above), only costs which are included in an approved periodic report will be taken into account.

The Agency will calculate the final grant amount (see Article 5.3) and the balance (see Article 21.4) on the basis of the reports submitted. Only costs incurred until termination are eligible (see Article 6). Costs relating to contracts due for execution only after termination are not eligible.

Improper termination may lead to a reduction of the grant (see Article 43).

After termination, the beneficiaries’ obligations (in particular Articles 20, 22, 23, Section 3 of Chapter 4, 36, 37, 38 and 40) continue to apply.

50.2 Termination of participation for one or more beneficiaries, by the beneficiaries

50.2.1 Conditions and procedure

The participation of one or more beneficiaries may be terminated by the coordinator, on request of the beneficiary concerned or on behalf of the other beneficiaries.

The coordinator must formally notify termination to the Agency (see Article 52) and inform the beneficiary concerned.

If the coordinator’s participation is terminated without its agreement, the formal notification must be done by another beneficiary (acting on behalf of the other beneficiaries).

The notification must include:

- the reasons why;

- the opinion of the beneficiary concerned (or proof that this opinion has been requested in writing);

- the date the termination takes effect. This date must be after the notification, and

- a request for amendment (see Article 55), with a proposal for reallocation of the tasks and the estimated budget of the beneficiary concerned (see Annexes 1 and 2) and, if necessary, the addition of one or more new beneficiaries (see Article 56). If termination takes effect after the period set out in Article 3, no request for amendment must be included unless the beneficiary concerned is the coordinator. In this case, the request for amendment must propose a new coordinator.

If this information is not given or if the Agency considers that the reasons do not justify termination, the participation will be considered to have been terminated improperly.

The termination will take effect on the day specified in the notification.

50.2.2 Effects

The coordinator must — within 30 days from when termination takes effect — submit:

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(i) a report on the distribution of payments to the beneficiary concerned and

(ii) if termination takes effect during the period set out in Article 3, a ‘termination report’ from the beneficiary concerned, for the open reporting period until termination, containing an overview of the progress of the work, an overview of the use of resources, the individual financial statement and, if applicable, the certificate on the financial statement (see Article 20.3 and 20.4).

The information in the termination report must also be included in the periodic report for the next reporting period (see Article 20.3).

If the request for amendment is rejected by the Agency, because it calls into question the decision awarding the grant or breaches the principle of equal treatment of applicants the Agreement may be terminated according to Article 50.3.1(c).

If the request for amendment is accepted by the Agency, the Agreement is amended to introduce the necessary changes (see Article 55).

The Agency will calculate — on the basis of the periodic reports, the termination report and the report on the distribution of payments — if the (pre-financing and interim) payments received by the beneficiary concerned exceed the beneficiary’s EU contribution (calculated by applying the reimbursement rate(s) to the eligible costs declared by the beneficiary and its linked third parties and approved by the Agency). Only costs incurred by the beneficiary concerned until termination takes effect are eligible (see Article 6). Costs relating to contracts due for execution only after termination are not eligible.

• If the payments received exceed the amounts due:

- if termination takes effect during the period set out in Article 3 and the request for amendment is accepted, the beneficiary concerned must repay to the coordinator the amount unduly received. The Agency will formally notify the amount unduly received and request the beneficiary concerned to repay it to the coordinator within 30 days of receiving notification. If it does not repay the coordinator, the Agency will draw upon the Guarantee Fund to pay the coordinator and then notify a debit note on behalf of the Guarantee Fund to the beneficiary concerned (see Article 44);

- in all other cases (in particular if termination takes effect after the period set out in Article 3), the Agency will formally notify a debit note to the beneficiary concerned. If payment is not made by the date in the debit note, the Guarantee Fund will pay to the Agency the amount due and the Agency will notify a debit note on behalf of the Guarantee Fund to the beneficiary concerned (see Article 44);

- if the beneficiary concerned is the former coordinator, it must repay the new coordinator according to the procedure above, unless:

- termination is after an interim payment and

- the former coordinator has not distributed amounts received as pre-financing or interim payments (see Article 21.7).

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In this case, the Agency will formally notify a debit note to the former coordinator. If payment is not made by the date in the debit note, the Guarantee Fund will pay to the Agency the amount due. The Agency will then pay the new coordinator and notify a debit note on behalf of the Guarantee Fund to the former coordinator (see Article 44).

• If the payments received do not exceed the amounts due: amounts owed to the beneficiary concerned will be included in the next interim or final payment.

If the Agency does not receive the termination report within the deadline (see above), only costs included in an approved periodic report will be taken into account.

If the Agency does not receive the report on the distribution of payments within the deadline (see above), it will consider that:

- the coordinator did not distribute any payment to the beneficiary concerned and that

- the beneficiary concerned must not repay any amount to the coordinator.

Improper termination may lead to a reduction of the grant (see Article 43) or termination of the Agreement (see Article 50).

After termination, the concerned beneficiary’s obligations (in particular Articles 20, 22, 23, Section 3 of Chapter 4, 36, 37, 38 and 40) continue to apply.

50.3 Termination of the Agreement or participation for one or more beneficiaries, by the Agency

50.3.1 Conditions

The Agency may terminate the Agreement or the participation of one or more beneficiaries, if:

(a) one or more beneficiaries do not accede to the Agreement (see Article 56);

(b) a change to their legal, financial, technical, organisational or ownership situation (or those of its linked third parties) is likely to substantially affect or delay the implementation of the action or calls into question the decision to award the grant;

(c) following termination of participation for one or more beneficiaries (see above), the necessary changes to the Agreement would call into question the decision awarding the grant or breach the principle of equal treatment of applicants (see Article 55);

(d) implementation of the action is prevented by force majeure (see Article 51) or suspended by the coordinator (see Article 49.1) and either:

(i) resumption is impossible, or

(ii) the necessary changes to the Agreement would call into question the decision awarding the grant or breach the principle of equal treatment of applicants;

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(e) a beneficiary is declared bankrupt, being wound up, having its affairs administered by the courts, has entered into an arrangement with creditors, has suspended business activities, or is subject to any other similar proceedings or procedures under national law;

(f) a beneficiary (or a natural person who has the power to represent or take decisions on its behalf) has been found guilty of professional misconduct, proven by any means;

(g) a beneficiary does not comply with the applicable national law on taxes and social security;

(h) the action has lost scientific or technological relevance;

(i) not applicable;

(j) not applicable;

(k) a beneficiary (or a natural person who has the power to represent or take decisions on its behalf) has committed fraud, corruption, or is involved in a criminal organisation, money laundering or any other illegal activity affecting the EU’s financial interests;

(l) a beneficiary (or a natural person who has the power to represent or take decisions on its behalf) has — in the award procedure or under the Agreement — committed:

(i) substantial errors, irregularities, fraud or

(ii) serious breach of obligations, including improper implementation of the action, submission of false information, failure to provide required information, breach of ethical principles;

(m) a beneficiary has committed — in other EU or Euratom grants awarded to it under similar conditions — systemic or recurrent errors, irregularities, fraud or serious breach of obligations that have a material impact on this grant (‘extension of findings from other grants to this grant’).

50.3.2 Procedure

Before terminating the Agreement or participation of one or more beneficiaries, the Agency will formally notify the coordinator:

- informing it of its intention to terminate and the reasons why and

- inviting it, within 30 days of receiving notification, to submit observations and — in case of Point (l.ii) above — to inform the Agency of the measures to ensure compliance with the obligations under the Agreement.

If the Agency does not receive observations or decides to pursue the procedure despite the observations it has received, it will formally notify to the coordinator confirmation of the termination and the date it will take effect. Otherwise, it will formally notify that the procedure is not continued.

The termination will take effect:

- for terminations under Points (b), (c), (e), (g), (h), (j), and (l.ii) above: on the day specified in the notification (see above);

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- for terminations under Points (a), (d), (f), (i), (k), (l.i) and (m) above: on the day after notification is received by the coordinator.

50.3.3 Effects

(a) for termination of the Agreement:

The coordinator must — within 60 days from when termination takes effect — submit:

(i) a periodic report (for the last open reporting period until termination; see Article 20.3) and

(ii) a final report (see Article 20.4).

If the Agreement is terminated for breach of the obligation to submit the reports (see Articles 20.8 and 50.3.1(l)), the coordinator may not submit any reports after termination.

If the Agency does not receive the reports within the deadline (see above), only costs which are included in an approved periodic report will be taken into account.

The Agency will calculate the final grant amount (see Article 5.3) and the balance (see Article 21.4) on the basis of the reports submitted. Only costs incurred until termination takes effect are eligible (see Article 6). Costs relating to contracts due for execution only after termination are not eligible.

This does not affect the Agency's right to reduce the grant (see Article 43) or to impose administrative and financial penalties (Article 45).

The beneficiaries may not claim damages due to termination by the Agency (see Article 46).

After termination, the beneficiaries’ obligations (in particular Articles 20, 22, 23, Section 3 of Chapter 4, 36, 37, 38 and 40) continue to apply.

(b) for termination of the participation of one or more beneficiaries:

The coordinator must — within 60 days from when termination takes effect — submit:

(i) a report on the distribution of payments to the beneficiary concerned;

(ii) a request for amendment (see Article 55), with a proposal for reallocation of the tasks and estimated budget of the beneficiary concerned (see Annexes 1 and 2) and, if necessary, the addition of one or more new beneficiaries (see Article 56). If termination is notified after the period set out in Article 3, no request for amendment must be submitted unless the beneficiary concerned is the coordinator. In this case the request for amendment must propose a new coordinator, and

(iii) if termination takes effect during the period set out in Article 3, a termination report from the beneficiary concerned, for the open reporting period until termination, containing an overview of the progress of the work, an overview of the use of resources,

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the individual financial statement and, if applicable, the certificate on the financial statement (see Article 20).

The information in the termination report must also be included in the periodic report for the next reporting period (see Article 20.3).

If the request for amendment is rejected by the Agency because it calls into question the decision awarding the grant or breaches the principle of equal treatment of applicants, the Agreement may be terminated according to Article 50.3.1(c).

If the request for amendment is accepted by the Agency, the Agreement is amended to introduce the necessary changes (see Article 55).

• If the payments received exceed the amounts due:

- in all other cases, in particular if termination takes effect after the period set out in Article 3, the Agency will formally notify a debit note to the beneficiary concerned. If payment is not made by the date in the debit note, the Guarantee Fund will pay to the Agency the amount due and the Agency will notify a debit note on behalf of the Guarantee Fund to the beneficiary concerned (see Article 44);

- if the beneficiary concerned is the former coordinator, it must repay the new coordinator the amount unduly received, unless:

- termination takes effect after an interim payment and

- the former coordinator has not distributed amounts received as pre- financing or interim payments (see Article 21.7)

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notify a debit note on behalf of the Guarantee Fund to the former coordinator (see Article 44).

• If the payments received do not exceed the amounts due: amounts owed to the beneficiary concerned will be included in the next interim or final payment.

If the Agency does not receive the termination report within the deadline (see above), only costs included in an approved periodic report will be taken into account.

If the Agency does not receive the report on the distribution of payments within the deadline (see above), it will consider that:

- the coordinator did not distribute any payment to the beneficiary concerned, and that

- the beneficiary concerned must not repay any amount to the coordinator.

After termination, the concerned beneficiary’s obligations (in particular Articles 20, 22, 23, Section 3 of Chapter 4, 36, 37, 38 and 40) continue to apply.

SECTION 4 FORCE MAJEURE

ARTICLE 51 — FORCE MAJEURE

51.1 Force majeure

‘Force majeure’ means any situation or event that:

- prevents either party from fulfilling their obligations under the Agreement,

- was unforeseeable, exceptional situation and beyond the parties’ control,

- was not due to error or negligence on their part (or on the part of third parties involved in the action), and

- proves to be inevitable in spite of exercising all due diligence.

The following cannot be invoked as force majeure:

- any default of a service, defect in equipment or material or delays in making them available, unless they stem directly from a relevant case of force majeure,

- labour disputes or strikes, or

- financial difficulties.

Any situation constituting force majeure must be formally notified to the other party without delay, stating the nature, likely duration and foreseeable effects.

The parties must immediately take all the necessary steps to limit any damage due to force majeure and do their best to resume implementation of the action as soon as possible.

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The party prevented by force majeure from fulfilling its obligations under the Agreement cannot be considered in breach of them.

CHAPTER 7 FINAL PROVISIONS

ARTICLE 52 — COMMUNICATION BETWEEN THE PARTIES

52.1 Form and means of communication

Communication under the Agreement (information, requests, submissions, ‘formal notifications’, etc.) must:

- be made in writing and

- bear the number of the Agreement.

Until the payment of the balance: all communication must be made through the electronic exchange system and using the forms and templates provided there.

After the payment of the balance: formal notifications must be made by registered post with proof of delivery (‘formal notification on paper’).

Communications in the electronic exchange system must be made by persons authorised according to the ‘Terms and Conditions of Use of the electronic exchange system’. For naming the authorised persons, each beneficiary must have designated to the Agency — before the signature of this Agreement — a ‘Legal Entity Appointed Representative (LEAR)’. The role and tasks of the LEAR are stipulated in his/her appointment letter (see Terms and Conditions of Use of the electronic exchange system).

If the electronic exchange system is temporarily unavailable, instructions will be given on the Agency's websites.

52.2 Date of communication

Communications are considered to have been made when they are sent by the sending party (i.e. on the date and time they are sent through the electronic exchange system).

Formal notifications through the electronic exchange system are considered to have been made when they are received by the receiving party (i.e. on the date and time of acceptance by the receiving party, as indicated by the time stamp). A formal notification that has not been accepted within 10 days after sending is considered to have been accepted.

Formal notifications on paper sent byregistered post with proof of delivery (only after the payment of the balance) are considered to have been made on either:

- the delivery date registered by the postal service or

- the deadline for collection at the post office.

If the electronic exchange system is temporarily unavailable, the sending party cannot be considered in breach of its obligation to send a communication within a specified deadline.

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52.3 Addresses for communication

The electronic exchange system must be accessed via the following URL:

https://ec.europa.eu/research/participants/portal/desktop/en/projects/

The Agency will formally notify the coordinator and beneficiaries in advance any changes to this URL.

Formal notifications on paper (only after the payment of the balance) addressed to the Agency must be sent to the following address:

Research Executive Agency (REA) Sustainable Resources for Food Security and Growth COV 2 - B2 Single Entry point B-1049 Brussels Belgium

Formal notifications on paper (only after the payment of the balance) addressed to the beneficiaries must be sent to their legal address as specified in the Beneficiary Register (in the electronic exchange system).

ARTICLE 53 — INTERPRETATION OF THE AGREEMENT

53.1 Precedence of the Terms and Conditions over the Annexes

The provisions in the Terms and Conditions of the Agreement take precedence over its Annexes.

The provisions in Annex 2 take precedence over Annex 1.

53.2 Privileges and immunities not applicable

ARTICLE 54 — CALCULATION OF PERIODS, DATES AND DEADLINES

In accordance with Regulation No 1182/7128, periods expressed in days, months or years are calculated from the moment the triggering event occurs.

The day during which that event occurs is not considered as falling within the period.

ARTICLE 55 — AMENDMENTS TO THE AGREEMENT

55.1 Conditions

The Agreement may be amended, unless the amendment entails changes to the Agreement which would call into question the decision awarding the grant or breach the principle of equal treatment of applicants.

Amendments may be requested by any of the parties.

28 Regulation (EEC, Euratom) No 1182/71 of the Council of 3 June 1971 determining the rules applicable to periods, dates and time-limits (OJ L 124, 8.6.1971, p. 1).

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55.2 Procedure

The party requesting an amendment must submit a request for amendment signed in the electronic exchange system (see Article 52).

The coordinator submits and receives requests for amendment on behalf of the beneficiaries (see Annex 3).

If a change of coordinator is requested without its agreement, the submission must be done by another beneficiary (acting on behalf of the other beneficiaries).

The request for amendment must include:

- the reasons why;

- the appropriate supporting documents;

- for a change of coordinator without its agreement: the opinion of the coordinator (or proof that this opinion has been requested in writing).

The Agency may request additional information.

If the party receiving the request agrees, it must sign the amendment in the electronic exchange system within 45 days of receiving notification (or any additional information the Agency has requested). If it does not agree, it must formally notify its disagreement within the same deadline. The deadline may be extended, if necessary for the assessment of the request. If no notification is received within the deadline, the request is considered to have been rejected

An amendment enters into force on the day of the signature of the receiving party.

An amendment takes effect on the date agreed by the parties or, in the absence of such an agreement, on the date on which the amendment enters into force.

ARTICLE 56 — ACCESSION TO THE AGREEMENT

56.1 Accession of the beneficiaries mentioned in the Preamble

The other beneficiaries must accede to the Agreement by signing the Accession Form (see Annex 3) in the electronic exchange system (see Article 52), within 30 days after its entry into force (see Article 58).

They will assume the rights and obligations under the Agreement with effect from the date of its entry into force (see Article 58).

If a beneficiary does not accede to the Agreement within the above deadline, the coordinator must — within 30 days — request an amendment to make any changes necessary to ensure proper implementation of the action. This does not affect the Agency's right to terminate the Agreement (see Article 50).

56.2 Addition of new beneficiaries

In justified cases, the beneficiaries may request the addition of a new beneficiary.

84 Grant Agreement number: 634476 — TREASURE — H2020-SFS-2014-2015/H2020-SFS-2014-2

For this purpose, the coordinator must submit a request for amendment in accordance with Article 55. It must include an Accession Form (see Annex 3) signed by the new beneficiary in the electronic exchange system (see Article 52).

New beneficiaries must assume the rights and obligations under the Agreement with effect from the date of their accession specified in the Accession Form (see Annex 3).

ARTICLE 57 — APPLICABLE LAW AND SETTLEMENT OF DISPUTES

57.1 Applicable law

The Agreement is governed by the applicable EU law, supplemented if necessary by the law of Belgium.

57.2 Dispute settlement

If a dispute concerning the interpretation, application or validity of the Agreement cannot be settled amicably, the General Court — or, on appeal, the Court of Justice of the European Union — has sole jurisdiction. Such actions must be brought under Article 272 of the Treaty on the Functioning of the EU (TFEU).

As an exception, if such a dispute is between the Agency and INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN, FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE, the competent Belgian courts have sole jurisdiction.

If a dispute concerns offsetting or an enforceable decision under Article 299 TFEU (see Articles 44, 45 and 46), the beneficiaries must bring action before the General Court — or, on appeal, the Court of Justice of the European Union — under Article 263 TFEU.

ARTICLE 58 — ENTRY INTO FORCE OF THE AGREEMENT

The Agreement will enter into force on the day of signature by the Agency or the coordinator, depending on which is later.

SIGNATURES

For the coordinator For the Agency

[--TGSMark#signature-999466273_75_210--] [--TGSMark#signature-service_75_210--]

85 European Commission Research Executive Agency (REA)

Sustainable Resources for Food Security and Growth

ANNEX 1 (part A)

Research and Innovation action

NUMBER — 634476 — TREASURE Table of Contents

1.1. The project summary...... 3 1.2. The list of beneficiaries...... 4 1.3. Workplan Tables - Detailed implementation...... 6 1.3.1. WT1 List of work packages...... 6 1.3.2. WT2 List of deliverables...... 7 1.3.3. WT3 Work package descriptions...... 15 Work package 1...... 15 Work package 2...... 25 Work package 3...... 32 Work package 4...... 41 Work package 5...... 48 Work package 6...... 56 1.3.4. WT4 List of milestones...... 60 1.3.5. WT5 Critical Implementation risks and mitigation actions...... 65 1.3.6 WT6 Summary of project effort in person-months...... 68 1.3.7. WT7 Tentative schedule of project reviews...... 70 1.4. Ethics Requirements...... 71 1.1. The project summary

Project Number 1 634476 Project Acronym 2 TREASURE

One form per project General information DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH Project title 3 QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS

Starting date 4 01/04/2015

Duration in months 5 48

Call (part) identifier 6 H2020-SFS-2014-2 SFS-07a-2014 Topic Traditional resources for agricultural diversity and the food chain Fixed EC Keywords Animal genetic resources, Environment, resources and sustainability, Agrobiodiversity Free keywords traditional meat products; local feed resources; husbandry systems; pig;

Abstract 7 Research and development activities are proposed for the benefit of sustainable pork chains based on European local pig breeds and their production systems. Workprogramme is planned to respond to consumer demands for quality and healthiness of pork products with regional identity and societal demands for environment preservation and development of local agro-economy. Description and evaluation of local pig breeds, with an emphasis on untapped ones will be performed using novel genomic tools. Performance of local pig breeds will be evaluated in contrasted agro-geo-climatic conditions and production systems (indoor, outdoor, organic). Focus will be on pig feeding and management strategies and on the use of locally available feeding resources. Intrinsic quality of traditional and new regional high quality pork products and attitudes of consumers from various market areas will be assessed; in particular the motives for the choice and willingness to pay such products. Marketing strategies will be adressed in particular short chain distribution channels. All activities will be driven from the perspective of sustainability (environmental impact, animal welfare, product quality, consumer acceptability and market potential). The activities will engage innovative approaches to answer socio-economic demands of regional pork chains involving partners from different sectors. The ambition is to enhance existing and create new networks between academia and non- academia partners, within and between regions and to tackle the value chain for regional high quality pork products, focusing on diverse and so far untapped pig breeds, their production systems and pork products. Cross-fertilising interactions between research, local agriculture, businesses and end-users will be achieved with partners from these complementary sectors in all research and development activities.

Page 3 of 71 1.2. List of Beneficiaries

Project Number 1 634476 Project Acronym 2 TREASURE

List of Beneficiaries

Project Project No Name Short name Country entry exit month8 month KMETIJSKI INSTITUT SLOVENIJE - 1 KIS Slovenia 1 48 AGRICULTURAL INSTITUTE OF SLOVENIA 2 UNIVERZA V LJUBLJANI UL Slovenia 1 48 KMETIJSKO GOZDARSKA ZBORNICA 3 SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD KGZS-NM Slovenia 1 48 NOVO MESTO 4 IFIP-INSTITUT DU PORC ASSOCIATION IFIP France 1 48 INSTITUT NATIONAL DE LA RECHERCHE 5 INRA France 1 48 AGRONOMIQUE BAUERLICHE ERZEUGERGEMEINSCHAFT 6 BESH Germany 1 48 SCHWABISCH HALL WV SVEUCILISTE U ZAGREBU AGRONOMSKI 7 UNIZG Croatia 1 48 FAKULTET SVEUCILISTE JOSIPA JURJA STROSSMAYERA 8 U OSIJEKU POLJOPRIVREDNI FAKULTET U PFOS Croatia 1 48 OSIJEKU AGRIS SARDEGNA - AGENZIA PER LA 9 AGRIS Italy 1 48 RICERCA IN AGRICOLTURA ASSOCIAZIONE NAZIONALE ALLEVATORI 10 ANAS Italy 1 48 SUINI 11 UNIVERSITA DEGLI STUDI DI FIRENZE UNIFI Italy 1 48 CENTRI REGIONALI PER LE 12 CERTA Italy 1 48 TECNOLOGIEAGROALIMENTARI SCARL ALMA MATER STUDIORUM - UNIVERSITA DI 13 UNIBO Italy 1 48 BOLOGNA STAZIONE SPERIMENTALE PER L'INDUSTRIA 14 SSICA Italy 1 48 DELLE CONSERVE ALIMENTARI LIETUVOS SVEIKATOS MOKSLU 15 LUHS Lithuania 1 48 UNIVERSITETAS INSTITUTO POLITECNICO DE VIANA DE 16 IPVC Portugal 1 48 CASTELO 17 UNIVERSIDADE DE EVORA UEVORA Portugal 1 48 INSTITUT ZA STOCARSTVO BEOGRAD- 18 IAH Serbia 1 48 ZEMUN FACULTY OF AGRICULTURE - UNIVERSITY 19 UNIBG Serbia 1 48 OF BELGRADE INSTITUTO NACIONAL DE INVESTIGACION Y 20 INIA Spain 1 48 TECNOLOGIA AGRARIA Y ALIMENTARIA

Page 4 of 71 1.2. List of Beneficiaries

Project Project No Name Short name Country entry exit month8 month AGENCIA ESTATAL CONSEJO SUPERIOR DE 21 CSIC Spain 1 48 INVESTIGACIONES CIENTIFICAS CENTRE DE RECERCA EN ECONOMIA I 22 DESENVOLUPAMENT AGROALIMENTARI- CREDA Spain 1 48 UPC-IRTA CENTRO DE INVESTIGACIONES CIENTIFICAS 23 CICYTEX Spain 1 48 Y TECNOLOGICAS DE EXTREMADURA INSTITUT DE RECERCA I TECNOLOGIA 24 IRTA Spain 1 48 AGROALIMENTARIES ASOCIACION ESPANOLA DE CRIADORES DE 25 AECERIBER Spain 1 48 CERDO IBERICO

Page 5 of 71 1.3. Workplan Tables - Detailed implementation

1.3.1. WT1 List of work packages

Person- Start End WP Number 9 WP Title Lead beneficiary 10 months 11 month 12 month 13 Phenotypic and genetic characterization of regional WP1 13 - UNIBO 108.00 1 48 autochthonous pig populations in Europe Performance and management of WP2 local pig breeds in their production 1 - KIS 157.93 1 48 systems Traditional and new high quality WP3 5 - INRA 128.50 1 48 products with regional identity Consumer preferences and market WP4 24 - IRTA 103.65 1 48 research for sustainable pork chains WP5 Measures to maximise impact 12 - CERTA 79.88 1 48 WP6 Management 1 - KIS 53.14 1 48 Total 631.10

Page 6 of 71 1.3.2. WT2 list of deliverables

Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 List of relevant contacts and publications for D1.1 WP1 13 - UNIBO Report Public 18 autochthonous European pig populations List with details of genotyping data already available for regional D1.2 WP1 13 - UNIBO Report Public 18 autochthonous pig population in Europe with contacts and details Report on the dimension of the pig populations, D1.3 WP1 13 - UNIBO Report Public 18 including phenotypic characterization of the animals Confidential, only for members Repository of DNA of the consortium D1.4 WP1 13 - UNIBO Other 20 samples collected (including the Commission Services) List of the most interesting major genes and D1.5 WP1 13 - UNIBO Report Public 24 polymorphisms for the studied autochthonous breeds/population A report on genetic diversity of pig population considered Confidential, for DNA sampling only for members with integration of the consortium D1.6 WP1 13 - UNIBO Report 36 with information (including the on population with Commission genotyping data Services) already available outside of the project Confidential, only for members Sequence data on 20 of the consortium D1.7 genomes resequenced WP1 13 - UNIBO Other 36 (including the in pools Commission Services)

Page 7 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 Confidential, Protocols for the only for members identification of of the consortium D1.8 WP1 13 - UNIBO Demonstrator 36 informative markers (including the for breed traceability Commission Services) A list of informative Confidential, SNPs for the analyzed only for members populations and of the consortium D1.9 WP1 13 - UNIBO Report 48 development of (a) (including the specific genotyping Commission tool(s) Services) Confidential, Websites, only for members Registration of patents of the consortium D1.10 WP1 13 - UNIBO 48 possible patents filling, (including the etc. Commission Services) Confidential, only for members RNA-seq data in of the consortium D1.11 WP1 13 - UNIBO Other 48 several pig tissues (including the Commission Services) List of genes upregulated or Confidential, downregulated in the only for members investigated tissues of the consortium D1.12 WP1 13 - UNIBO Report 48 according to the (including the experimental designs Commission derived by RNA-seq Services) data Confidential, Microbiome data in only for members different breeds and of the consortium D1.13 production system; WP1 13 - UNIBO Report 48 (including the co-deliverable with Commission WP2.1 Services) List of bacteria Confidential, families found only for members according to the of the consortium D1.14 WP1 13 - UNIBO Report 48 experimental (including the designs derived by Commission microbiome analyses Services) Confidential, Compilation of the only for members data on productive of the consortium D2.1 WP2 1 - KIS Report 18 traits of local pig (including the breeds Commission Services) Page 8 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 Confidential, Compilation only for members of the data for of the consortium D2.2 evaluation of welfare, WP2 1 - KIS Report 19 (including the environmental impact Commission and economic output Services) Confidential, only for members Analytical review of of the consortium D2.3 production traits in WP2 1 - KIS Report 30 (including the local pig breeds Commission Services) Confidential, Report with results only for members from the studies of the consortium D2.4 WP2 1 - KIS Report 35 on innovative (including the management practices Commission Services) Confidential, only for members Report with results of the consortium D2.5 of trials with local WP2 1 - KIS Report 36 (including the feeding resources Commission Services) Confidential, Report with results only for members from the studies of the consortium D2.6 on nutritional WP2 1 - KIS Report 38 (including the requirements of local Commission pig breeds Services) Sheets with description of the studied production Confidential, systems and only for members innovative strategies of the consortium D2.7 WP2 1 - KIS Report 42 (average and (including the variability) regarding Commission the data used as Services) indicators for the tools used in task 2.2 Report on pilot Confidential, study: microbiota only for members composition in of the consortium D2.8 relation to pig breed WP2 1 - KIS Report 48 (including the and production system Commission – co-deliverable of Services) WP1.7 Report on the Confidential, D2.9 evaluation of studied WP2 1 - KIS Report only for members 48 production systems of the consortium Page 9 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 and innovative (including the strategies in regard to Commission welfare, environment, Services) economics Confidential, only for members Harmonized protocol of the consortium D3.1 for consumer studies WP3 5 - INRA Other 14 (including the in Europe (3.3) Commission Services) A toolbox for the Confidential, evaluation of sensory, only for members healthiness and of the consortium D3.2 WP3 5 - INRA Report 15 technological quality (including the of pork and pork Commission products. Services) Specifications of the D3.3 database and of the WP3 5 - INRA Report Public 18 exchange formats Report on consumer acceptability of Traditional pork products (TPP) from untapped breeds in Confidential, relation to Innovative only for members traditional pork of the consortium D3.4 WP3 5 - INRA Report 36 products (ITPP) (including the in 5 European Commission countries and Services) contrasted city-market zones, including comparison of TPP and conventional ones A report on the average and variation within breed/product of the sensory, healthy Confidential, and technological only for members qualities of pork of the consortium D3.5 WP3 5 - INRA Report 48 products from (including the various untapped Commission local breeds and Services) production systems from contrasted European regions A report on the Confidential, sensory, healthiness only for members and technological of the consortium D3.6 WP3 5 - INRA Report 48 qualities of innovative (including the traditional pork Commission products from local Services) Page 10 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 breeds and aiming improved healthy quality A report on the influence of feeding strategy and animal Confidential, management practices only for members on the carcass of the consortium D3.7 WP3 5 - INRA Report 48 composition and (including the sensory, healthy and Commission technological qualities Services) of pork and pork products Confidential, A report on prediction only for members methods of chemical of the consortium D3.8 composition of meat WP3 5 - INRA Report 48 (including the and pork products by Commission NIRs analysis Services) Database on Confidential, systematic carcass only for members and meat quality traits of the consortium D3.9 measurements in each WP3 5 - INRA Report 48 (including the local breed aiming Commission at implementing Services) breeding programmes Confidential, Standardised protocol only for members for collecting data at of the consortium D4.1 farm level for the Six WP4 24 - IRTA Demonstrator 8 (including the Production systems Commission involved Services) Updated and ungraded literature review on Confidential, public good valuation only for members methods to select of the consortium D4.2 WP4 24 - IRTA Report 12 the most appropriate (including the methodological Commission approach to our cases Services) studies Confidential, Harmonised protocol only for members for Choice experiment of the consortium D4.3 WP4 24 - IRTA Demonstrator 14 for the Six Production (including the systems involved Commission Services) Confidential, Standardized protocol only for members D4.4 for collecting data WP4 24 - IRTA Demonstrator 18 of the consortium at citizen level for (including the

Page 11 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 the Six Production Commission systems involved Services) Results of the choice Confidential, experiments to only for members estimate the total value of the consortium D4.5 WP4 24 - IRTA Report 30 of the public goods (including the produced in each Commission production system Services) Results of the Confidential, CostBenefit analysis only for members and results on of the consortium D4.6 WP4 24 - IRTA Report 36 heterogeneity between (including the the different analysed Commission regions Services) Confidential, Results of the Choice only for members test taking into of the consortium D4.7 account cross-cultural WP4 24 - IRTA Report 36 (including the differences between Commission the European regions Services) Confidential, Results of the Choice only for members test for Traditional of the consortium D4.8 products and WP4 24 - IRTA Report 36 (including the innovative traditional Commission products Services) Standardized survey Confidential, with guidelines to be only for members discussed in the focus of the consortium D4.9 groups for collecting WP4 24 - IRTA Demonstrator 36 (including the data at stakeholder Commission for the Six Production Services) systems involved Confidential, Results of the social only for members CostBenefit analysis of the consortium D4.10 WP4 24 - IRTA Report 42 by including costs and (including the benefit at farm level Commission Services) Confidential, only for members Qualitative results of of the consortium D4.11 the Q methodology WP4 24 - IRTA Report 45 (including the and Focus groups Commission Services) Review of year 1activities on D5.1 dissemination, WP5 12 - CERTA Report Public 13 demonstration, communication Page 12 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 Concept and rules D5.2 of the collective WP5 12 - CERTA Report Public 24 trademark Review of year 2 activities on D5.3 dissemination, WP5 12 - CERTA Report Public 25 demonstration, communication Local pig breeds and D5.4 WP5 12 - CERTA Report Public 28 products database Certificate of D5.5 registration of WP5 12 - CERTA Report Public 32 collective trademark Report on Training D5.6 school for dry cured WP5 12 - CERTA Report Public 34 products Good practice guide D5.7 on Sarda breed rearing WP5 12 - CERTA Other Public 35 and feeding systems Review of year 3 activities on D5.8 dissemination, WP5 12 - CERTA Report Public 37 demonstration, communication Review of year 4 activities on D5.9 dissemination, WP5 12 - CERTA Report Public 48 demonstration, communication Organisation of the D6.1 kick-off meeting; WP6 1 - KIS Report Public 1 Management plan Report formats for D6.2 technical support and WP6 1 - KIS Report Public 3 financial reports Confidential, only for members Data management of the consortium D6.3 plan; Collaborative WP6 1 - KIS Other 6 (including the platform ready Commission Services) Confidential, only for members TREASURE activity of the consortium D6.4 WP6 1 - KIS Report 18 report 1 (including the Commission Services)

Page 13 of 71 Due Deliverable WP Lead Dissemination Deliverable Title Type 15 Date (in Number 14 number 9 beneficiary level 16 months) 17 Confidential, only for members TREASURE activity of the consortium D6.5 WP6 1 - KIS Report 36 report 2 (including the Commission Services) Confidential, only for members TREASURE activity of the consortium D6.6 WP6 1 - KIS Report 48 report 3 (including the Commission Services)

Page 14 of 71 1.3.3. WT3 Work package descriptions

Work package number 9 WP1 Lead beneficiary 10 13 - UNIBO Phenotypic and genetic characterization of regional autochthonous pig populations in Work package title Europe Start month 1 End month 48

Objectives

• Describe the singularity of local pig breeds at phenotypic, genomic and functional levels • Evaluate the adaptation of local pig populations to agro-climatic conditions • Develop new methodologies for the management of the local pig populations • Develop DNA based tools for animal and meat traceability and authentication

Description of work and role of partners

WP1 - Phenotypic and genetic characterization of regional autochthonous pig populations in Europe [Months: 1-48] UNIBO, KIS, UL, IFIP, INRA, BESH, UNIZG, PFOS, AGRIS, ANAS, UNIFI, LUHS, IPVC, UEVORA, IAH, INIA, CSIC, CICYTEX, IRTA The WP aims to characterize the autochthonous local pig populations of Europe at different levels, from their phenotype to their genome, transcriptome and microbiome. Moreover, the analyses will include the characterization of specific production systems and management strategies linked to the local pig population involved in the TREASURE project.

Task 1.1 Phenotypic characterisation of regional autochthonous pig breeds with special attention to untapped populations

Partners: UNIFI (11, leader), KGZS-NM (3), IFIP (4), INRA (5), BESH (6), UNIZG (7), PFOS (8), AGRIS (9), ANAS (10), LUHS (15), IPVC (16), UEVORA (17), IAH (18), UNIBG (19), INIA (20) Start date and duration: month 1- 18 Linked deliverable(s): D1.1, D1.3

As a first step a specific design form will be prepared to investigate the situation of data already recorded in various regional autochthonous populations and local representative of breeders will be involved to collect and handle: a) the phenotypic characterization and demographical information with collecting data at herd book, register, herd or farm levels; b) already recorded, or possible to record within the Treasure project, traditional phenotypes, like morphological or production data to characterise the population (collected in WP2), c) blood or biological samples for all populations that do not have material already available for the project, with the aim of having at least 48 animals per each population. A list of key contacts for each local breed involved in the project will be created in order to encourage the building of a net of contacts necessary for the development of the project as well as for the final aim of TREASURE of creating a collective trade mark to behave as an umbrella of pig production from autochthonous population over Europe. Together with Task 2.1 of WP2, production traits already available, or eventually collected within WP2, will be evaluated by comparing production data of various local populations with performances of selected lines available from the literature data. Contact lists, information and wet and dry materials collected within this task will contribute both in the building of the network as well as feeding materials for subsequent tasks based on DNA analyses.

Participants and roles: Task leader UNIFI will coordinate the activities and especially ensure the collection of phenotypes and samples and the finalization of analyses as scheduled by the partners involved, including protocol/form for phenotypic characterisation and demographic information collection. Samples, phenotypic and demographical information will be collected from populations considered in the Treasure project with special emphasis on untapped breeds: Cinta Senese breed UNIFI (in cooperation with third party CONCS), KIS, IFIP French traditional breeds, BESH Schwäbish-Hällisches breed, UNIZG Turopolje breed (in cooperation with third party POT), PFOS Crna slavonska breed (in cooperation with third party LETA), AGRIS Sarda breed (in cooperation with third party UNISS, ANAS Italian local breeds, LUHS Lietuvos vietinės and old Lithuanian white breeds (in cooperation with third party LEFABA), IPVC Bisara breed (in cooperation with third party ANCSUB), UEVORA Alentejana breed (in cooperation with third party ANCPA), IAH Moravka and Mangulica breeds (in cooperation with third party DUD), INIA Ibérico breed, IRTA Mallorquí pig

Page 15 of 71 Task 1.2 Genetic analyses of the untapped pig populations with High Density SNP chips (MANAGEMENT TOOL) to determine basic genetic parameters.

Partners: INIA (20, leader), UNIBO (13), IRTA (24) Start date and duration: month 1- 36 Linked deliverable(s): D1.2, D1.4, D1.6

Aim of the task is the estimation of the variability and the genetic diversity of local pig populations based on high density SNP data analyses (commercial Porcine 60K SNP BeadChip, Illumina, Inc.) (MANAGEMENT TOOL). The genetical investigation of the untapped European pig populations with high density chips makes possible the estimation of different indicators as i.e. the relative homozygosity and the observed and expected genetic diversity of the studied local pig breeds, as well as the definition of the structure of meta-populations through the proportion of shared alleles/haplotypes among animals and breed or population relationships. By comparison with SNPs genotypes already available of other main pig populations in Europe, it will be feasible the identification of genome regions related to between-breed differentiation, or connected to evidence of selective sweeps or signatures of demographic events specific of these breeds. The populations/breeds that will be considered for genotyping are: Krškopolje, Gascon, Schwäbisch-Hällisches, Turopolje, Crna slavonska, Sarda, Mora Romagnola, Cinta Senese, Lietuvos vietinės, Senojo tipo Lietuvos baltosios, Bisara, Alentejana, Ribatejano, Mangulica, Moravka, Resavka, Negre Mallorquí Breed. Data on SNP already available will be provided by partners of Treasure project, as results of previous scientific researches, for Ibérico, Apulo Calabrese, Casertana and Nero Sicilano pig breeds that will not be re-genotyped. Moreover genotyping data related to wild boar, Ibérico, Duroc, Landrace, Large White and local pig breeds listed above will be also provided by partners and integrated in the project to be used for analyses of breeds/populations variability and for the genotyping comparison with pig breeds at large diffusion in Europe as well as with the wild boar. The task is based on the following steps: a) the check the availability of biological samples (from task 1.1), organize the eventual remaining necessary sampling and the extraction of DNA from at least 48 pigs DNA samples for the 18 before listed non-genotyped regional pig populations; b) the constitution of a list of already available PorcineSNP60 Beadchip data in local populations involved in the project; c) the DNA extraction and genotyping of samples collected with the PorcineSNP60 Beadchip; d) data analyses

Participants and roles: Task leader INIA will coordinate the activities, conduct the genotyping (Porcine 60K BeadChip) and ensure the collection of DNA samples, SNP data. SNP data collection and analyses will be conducted by INIA, UNIBO and IRTA.

Task 1.3 Allele frequencies of known major genes in regional autochthonous pig populations

Partners: INIA (20, leader), UNIBO (13), UL (2), PFOS (8) Start date and duration: month 1- 24 Linked deliverable(s): D1.5

Aim of the task is the investigation of the allele frequency distribution of known major genes in local pig populations through the analysis of causative polymorphisms or polymorphisms associated with other traits as determined in studies carried out in cosmopolitan breeds. The analysis would be conducted on genes regulating growth and appetite such as IGF2, MC4R and LEPR, genes regulating meat quality traits such as RYR1, PRKAG3 and CAST and genes determining coat colour such as MC1R and KIT, among others. The task is based on the following steps: a) in a first step, the most interesting major genes and polymorphisms to analyse will be identified for 23 regional autochthonous breeds/populations; b) after the identification, at least 5 polymorphisms will be genotyped for 48 pigs in each of the 23 populations, and biological material will be collected (together with task 1.2) and analysed accordingly; c) protocols for polymorphism genotyping will be set up and eventually previous available data on major genes on the same or other breeds/populations will be included in the analyses (e.g. Ibérico, Duroc, Landrace, wild boars, and other local population); d) data analysis. The pig population considered for the mutations in major or candidate genes polymorphism analyses are all of the 23 participating to the project: Krškopolje, Gascon , Schwäbisch-Hällisches, Turopolje, Crna slavonska, Sarda, Mora Romagnola, Cinta Senese, Lietuvos vietinės, Senojo tipo Lietuvos baltosios, Bisara, Alentejana, Ribatejano, Mangulica, Moravka, Resavka, Negre Mallorquí Breed, Ibérico, Mora Romagnola, Apulo Calabrese, Casertana, Nero Sicilano.

Participants and roles:

Page 16 of 71 Task leader INIA will coordinate the activities. Selection of interesting candidate polymorphisms in major genes, genotyping protocol design, DNA sample collection, genotyping and analysis will be conducted by INIA, UNIBO, UL, and PFOS.

Task 1.4 Identification of genome regions or genes responsible of adaptive traits and resilience through the comparison of genomes of local, well adapted breeds with the ones of the commercial breeds.

Partners: UNIBO (13, leader), UL (2), INRA (5), INIA(20) Start date and duration: month 1-36 Linked deliverable(s): D1.7

The task is aiming to identify the genome regions for all of the 23 pig populations participating to the project and obtained by sequencing the genome of 20 animals per breed. Sequencing will be carried out in pools and will include the following steps: a) DNA extraction from samples collected in task 1.1; b) genome resequencing with the Illumina platform (at least 20X) with DNA genetic pooling analyses; c) data analysis. Analyses will interest a total of 22 DNA pools of 20 animals each for every of all the populations identified within the project populations with the only exception of the Ibérico breed that it is already whole genome sequenced and therefore the data will be integrated within the sequencing data of Treasure project. For the other populations, whole genome raw data will be generated from paired-end libraries for each sample. Multiplex sequencing will be conducted on an Illumina Hi-Seq 2000, according to the manufacturer’s instructions. Raw data will be then processed using bioinformatics tools to extract biologically useful information. Reads will be mapped against the latest reference genome (actually assembly Sscrofa 10.2) and after polymorphisms calling will be performed with standard quality and filtering criteria. Analyses will be focused on population adaptation and resilience signatures and will be conducted based on sweep screen (Rubin et al., 2012) and integrating free available whole genome sequences from other porcine breeds and wild boars. Few markers in the regions with particular selection signatures would be selected for genotyping in a larger number of breeds/individuals to verify the results obtained from the whole genome sequencing approach. Bioinformatics analyses will also contribute to clarify the role of genomic regions with selection signature looking at the annotations in these regions. Such data are expected to provide useful markers for traceability.

Participants and roles: Task leader UNIBO will coordinate the activities, conduct the DNA sequencing and ensure the collection of whole genome sequence data. Bioinformatics and statistics analyses will be conducted by UNIBO, UL, INRA and INIA.

Task 1.5 Identification of DNA markers useful for breed authentication and traceability

Partners: UNIBO (13, leader), UL (2), INRA (5), UNIFI (11), INIA (20), IRTA (24) Start date and duration: month 12- 48 Linked deliverable(s): D1.8, D1.9, D1.10

The task aim, together with WP3, to propose guidelines for a system of management of local autochthonous pig populations based on the information available from genotyping data. Actually most of the local pig population do not have pedigree recording systems making by consequence impossible any breeding strategy to preserve the breeds/ populations while controlling the inbreeding trend, that it is a great concern in populations with small number of breeding animals, and preserving the identity of local populations. Genotyping data make that a possibility also without the recording of pedigree data (recording that it is often difficult in breeding situations of local European pig populations) and this task, together with the demonstration activities of the project of WP5, aims in exploiting the great potentiality of Porcine 60K SNP BeadChip data and genome sequencing to be applied in genetic services (that will demonstrated in WP5) in support to management of autochthonous pig European populations. Together with WP3 tasks, this task aims to the: i) Exploitation of Porcine 60K SNP BeadChip data and candidate gene SNP data; ii) Exploitation of genome sequencing data; iii) Statistical approaches; iv) development of a cheap genotyping tool containing small sets of informative SNPs. From the existing strategies of breed/population management, well developed for large populations of various species, the most suitable ones for the goal of the project will be test for at least a local pig population as example for the management of local EU pig populations. The following steps will be considered: a) Data mining of the Porcine 60K SNP BeadChip data and candidate gene SNP data (data from Tasks 1.2, 1.3); b) Mining of the genome sequencing data (data fromTask 1.4);

Page 17 of 71 c) Evaluation of the appropriate statistical approach (PCA, Candisc analysis, random forest, etc.); d) Identification of the most informative markers for the different populations and useful for specific cases e) Identification of the minimum number of SNP for breed allocation; f) Application of at least one of the strategies for populations management based on genotyping data already proposed in literature to at least one specific pig local population as a pilot proposal for the management of these breeds; g) Test in specific field conditions the usefulness of the genotyping tool developed; h) Evaluate patenting issues (patents already present in this field and patents to be developed during the project in this field).

Participants and roles: Task leader UNIBO will coordinate the activities. Genotyping tool development and testing, data analysis and statistics will be conducted by UNIBO, UL, INRA, UNIFI, INIA and IRTA.

Task 1.6 Pilot functional studies through gene expression analyses

Partners: INIA (20, leader), UL (2), IAH (18), CSIC (21), CICYTEX (23) Start date and duration: month 24- 48 Linked deliverable(s): D1.11, D1.12

Pilot functional study through gene expression analyses would be conducted in the most developed pig populations participating to the project in order to identify population-specific biological processes responsible for production traits and product quality. Identification of gene expression changes conditional on diets, crosses and inmunocastration would be conducted using RNA-Seq technologies (link with WP2). The validation of results on other pig populations will be carried out by quantitative PCR. The proposal would allow the identification of the key genes responsible of the biological processes conditional on diets and immunocastration experiments (with interaction with WP2). The following two main experiments will be performed: Experiment 1: Evaluation of pig castration methodology applicable to the production of heavy local pigs, immunocastration, and their effects on production traits and product quality. The objective of this assay will be to determine transcriptome changes driven by inmunocastration vs surgical castration 1.1-The transcriptome of 5 samples from immunocastrated males will be compared to 5 surgical castration males in Ibérico pigs. Performance, growth related parameters (feed intake, ADG and feed efficiency) N flows, blood hormones and metabolites will be determined on these animals in by CSIC and CICYTEX partners in WP2. Tissue samples (liver, fat and Longissimus dorsi muscle) collection will be conducted at slaughter weight and stored in liquid nitrogen until RNA analyses. Analyses will be done with RNA-Seq analysis on muscle tissue and validations in the other tissues and breeds. 1.2- The transcriptome of 5 samples from immunocastrated males will be compared to 5 surgical castration males in Mangulica pigs. Performance, growth related parameters will be determined on these animals in by IAH partner in WP2. Tissue samples (liver, fat and Longissimus dorsi muscle) collection will be conducted at slaughter weight and stored in liquid nitrogen until RNA analyses. Analyses will be done with RNA-Seq analysis on muscle tissue and validations in the other tissues and breeds. Experiment 2: Evaluation of the effect of the use of special local feeding resources. The objective of this assay will be to determine muscle transcriptome changes driven by the introduction of special local feeding sources such as fiber in Iberian pigs and pasture and tannin rich wood sources in Mangulica pigs. 2.1. The transcriptome of 5 vs 5 samples from Ibérico males under diets differing in fiber supplement during the feed- restricted growing phase will be analyzed through RNA-Seq analysis on muscle. Performance, growth and meat quality related parameters will be determined on these animals in by CICYTEX partner in WP2. Tissue samples (liver, fat and Longissimus dorsi muscle) will be collected and stored in liquid nitrogen until RNA analyses. Analyses will be done with RNA-Seq analysis on muscle tissue and validations in the other tissues and more animals. 2.2 The transcriptome of 5 vs 5 Mangulica males fed with pasture and tannin rich wood sources will be analyzed through RNA-Seq analysis on muscle and fat. Tissue samples (liver, fat and Longissimus dorsi muscle) will be collected and stored in liquid nitrogen until RNA analyses. Analyses will be done with RNA-Seq analysis on muscle tissue and validations by qPCR in the other tissues and more animals. RNA-Seq raw data will be generated from paired-end libraries prepared using the TruSeq RNA Sample Prep Kit (Illumina) for each sample. Multiplex sequencing of the libraries (4 samples per line generating >20M reads per sample) will be conducted on an Illumina Hi-Seq 2000, according to the manufacturer’s instructions. The RNA-Seq data will be then processed using bioinformatics tools to extract biologically useful information.

Page 18 of 71 The task is based on the following steps: a) the collection of tissue samples; b) RNA extraction and quantity and quality measures; c) RNA-Sequencing d) RNA-Seq data analyses; e) qPCR validations.

Participants and roles: The task leader INIA will coordinate the activities, design protocol for sample collection and sequencing. qPCR assays, bioinformatics data analysis and statistics will be conducted by INIA (20) and UL (2). Samples will be collected from experiments in WP2: • IAH: pasture and tannin rich wood sources in Mangulica pigs • CSIC: immunocastration in Ibérico pigs • CYCITEX: fiber supplementation in Ibérico pigs

Task 1.7 Pilot characterisation of intestinal microbiota

Partners: INRA (5, leader), KIS (1), KGZS-NM (3), IAH (18), UNBG (19), INIA (20) Start date and duration: month 12- 48 Linked deliverable(s): D1.13 (co-deliverable with D2.8), D1.14

The intestinal microbiota is emerging as a fundamental factor in host’s health and homeostasis. Besides, host’s influence via its immune system, major quantitative and qualitative changes may occur in the composition of the intestinal microbiota thanks to the influence of diet and/or other environmental factors. The aim of this task is to conduct a pilot characterisation of intestinal microbiota in order to test its usefulness to characterize pig population and production systems in collaboration with WP2. Moreover, characterizing the differential metabolic capacities and response to perturbation will potentially inform strategies to improve gut health and food safety. Experiment 1. To test the usefulness of microbiome data to differentiate production systems 1.1 Microbiome will be characterized in Krškopolje pigs under conventional and organic production systems in collaboration with KIS and KGZS-NM partners (samples obtained in the experiment conducted in WP2). Faecal samples will be collected and stored until microbiome analysis. 1.2 Microbiome will be characterized in Ibérico pigs under two different diets: in “montanera” based on acorns and standard industrial feed. Faecal samples will be provided by partners of Treasure project as results of previous scientific researches focused on Ibérico pig production systems. The samples remain stored at -80C until microbiome analysis. Experiment 2. To test the usefulness of microbiome data to differentiate pig populations 2.1 Microbiome of Moravka and Mangulica pig breeds will be tested in the same environment and production system in collaboration with UNIBG, IAH and IMHT partners (samples obtained in the experiment conducted in WP2). Faecal samples will be collected and stored until microbiome analysis. In both experiments, characterisation will be done by profiling the 16S rRNA gene diversity with a massive parallel sequencing technology using an Illumina's MySeq. The task is based on the following steps: a) the collection of faecal samples for each experiment; b) DNA extraction; c) 16S amplification; d) DNA-Sequencing e) data analyses.

Participants and roles: The task leader will coordinate the activities, design protocol for sample collection and DNA-sequencing. Data analysis and statistics will be conducted by INRA and INIA. Samples will be collected from experiments conducted in WP2 on Krskopolje pigs (KIS + KGZS-NM), Moravka and Mangulica pigs (IAH + UNIBG), Ibérico

Participation per Partner

Partner number and short name WP1 effort 1 - KIS 2.06 2 - UL 26.01 4 - IFIP 0.50 5 - INRA 11.60 6 - BESH 0.50 7 - UNIZG 1.12 8 - PFOS 3.70

Page 19 of 71 Partner number and short name WP1 effort 9 - AGRIS 0.93 10 - ANAS 1.44 11 - UNIFI 8.05 13 - UNIBO 25.64 15 - LUHS 1.02 LEFABA 0.15 16 - IPVC 0.71 ANCSUB 0.13 17 - UEVORA 0.27 ANCPA 0.13 18 - IAH 3.32 20 - INIA 10.94 21 - CSIC 0.50 23 - CICYTEX 1.48 24 - IRTA 7.80 Total 108.00

List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 List of relevant contacts and publications for D1.1 13 - UNIBO Report Public 18 autochthonous European pig populations List with details of genotyping data already available for regional D1.2 13 - UNIBO Report Public 18 autochthonous pig population in Europe with contacts and details Report on the dimension of the pig populations, D1.3 including 13 - UNIBO Report Public 18 phenotypic characterization of the animals Repository of DNA Confidential, only D1.4 13 - UNIBO Other 20 samples collected for members of Page 20 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 the consortium (including the Commission Services) List of the most interesting major genes and D1.5 polymorphisms 13 - UNIBO Report Public 24 for the studied autochthonous breeds/population A report on genetic diversity of pig population considered for Confidential, only DNA sampling for members of with integration the consortium D1.6 13 - UNIBO Report 36 with information (including the on population with Commission genotyping data Services) already available outside of the project Confidential, only Sequence data for members of on 20 genomes the consortium D1.7 13 - UNIBO Other 36 resequenced in (including the pools Commission Services) Confidential, only Protocols for the for members of identification the consortium D1.8 of informative 13 - UNIBO Demonstrator 36 (including the markers for breed Commission traceability Services) A list of Confidential, only informative SNPs for members of for the analyzed the consortium D1.9 populations and 13 - UNIBO Report 48 (including the development of (a) Commission specific genotyping Services) tool(s) Confidential, only for members of Websites, Registration of the consortium D1.10 13 - UNIBO patents filling, 48 possible patents (including the etc. Commission Services)

Page 21 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Confidential, only for members of RNA-seq data in the consortium D1.11 13 - UNIBO Other 48 several pig tissues (including the Commission Services) List of genes upregulated or Confidential, only downregulated in for members of the investigated the consortium D1.12 13 - UNIBO Report 48 tissues according (including the to the experimental Commission designs derived by Services) RNA-seq data Microbiome data Confidential, only in different breeds for members of and production the consortium D1.13 13 - UNIBO Report 48 system; co- (including the deliverable with Commission WP2.1 Services) List of bacteria Confidential, only families found for members of according to the the consortium D1.14 experimental 13 - UNIBO Report 48 (including the designs derived Commission by microbiome Services) analyses

Description of deliverables

D1.1 List of relevant contacts and publications for autochthonous European pig populations (Month 18) D1.2 List with details of genotyping data already available for regional autochthonous pig population in Europe with contacts and details (Month 18) D1.3 Report on the dimension of the pig populations, including phenotypic characterization of the animals (Month 18) D1.4 Repository of DNA samples collected (Month 20) D1.5 List of the most interesting major genes and polymorphisms for the studied autochthonous breeds/population (Month 24) D1.6 A report on genetic diversity of pig population considered for DNA sampling with integration with information on population with genotyping data already available outside of the project (Month 36) D1.7 Sequence data on 20 genomes resequenced in pools (Month 36) D1.8 Protocols for the identification of informative markers for breed traceability (Month 36) D1.9 A list of informative SNPs for the analyzed populations and development of (a) specific genotyping tool(s) (Month 48) D1.10 Registration of possible patents (Month 48) D1.11 RNA-seq data in several pig tissues (Month 48) D1.12 List of genes upregulated or downregulated in the investigated tissues according to the experimental designs derived by RNA-seq data (Month 48) D1.13 Microbiome data in different breeds and production system; co-deliverable with WP2.1 (Month 48) D1.14 List of bacteria families found according to the experimental designs derived by microbiome analyses (Month 48) D1.1 : List of relevant contacts and publications for autochthonous European pig populations [18] List of relevant contacts and publications for autochthonous European pig populations D1.2 : List with details of genotyping data already available for regional autochthonous pig population in Europe with contacts and details [18] List with details of genotyping data already available for regional autochthonous pig population in Europe with contacts and details

Page 22 of 71 D1.3 : Report on the dimension of the pig populations, including phenotypic characterization of the animals [18] Report on the dimension of the pig populations, including phenotypic characterization of the animals D1.4 : Repository of DNA samples collected [20] Repository of DNA samples collected D1.5 : List of the most interesting major genes and polymorphisms for the studied autochthonous breeds/population [24] List of the most interesting major genes and polymorphisms for the studied autochthonous breeds/population D1.6 : A report on genetic diversity of pig population considered for DNA sampling with integration with information on population with genotyping data already available outside of the project [36] A report on genetic diversity of pig population considered for DNA sampling with integration with information on population with genotyping data already available outside of the project D1.7 : Sequence data on 20 genomes resequenced in pools [36] Sequence data on 20 genomes resequenced in pools D1.8 : Protocols for the identification of informative markers for breed traceability [36] Protocols for the identification of informative markers for breed traceability D1.9 : A list of informative SNPs for the analyzed populations and development of (a) specific genotyping tool(s) [48] A list of informative SNPs for the analyzed populations and development of (a) specific genotyping tool(s) D1.10 : Registration of possible patents [48] Registration of possible patents D1.11 : RNA-seq data in several pig tissues [48] RNA-seq data in several pig tissues D1.12 : List of genes upregulated or downregulated in the investigated tissues according to the experimental designs derived by RNA-seq data [48] List of genes upregulated or downregulated in the investigated tissues according to the experimental designs derived by RNA-seq data D1.13 : Microbiome data in different breeds and production system; co-deliverable with WP2.1 [48] Microbiome data in different breeds and production system; co-deliverable with WP2.1 D1.14 : List of bacteria families found according to the experimental designs derived by microbiome analyses [48] List of bacteria families found according to the experimental designs derived by microbiome analyses

Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Experiments confirmed, scheduled Schedule of experiments and data collection MS4 13 - UNIBO 4 and data collection protocols prepared, protocol questionnaire sent over Table with number of Sample collection for samples (and therefore MS18 13 - UNIBO 18 DNA studies ended animals sampled) collected per population Genotyping of major Basic statistics MS23 13 - UNIBO 24 genes on segregation Page 23 of 71 Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) polymorphisms and minor allele frequencies Tissue collection Table with id of samples MS28 13 - UNIBO 26 ended per population Feacal samples Table with id of samples MS31 13 - UNIBO 30 collection ended per experiment Genotyping with high MS37 13 - UNIBO 36 Table with results density chip Genomics analysis Table with statistic of MS38 13 - UNIBO 36 finished genomic analysis done Table with statistic Transcriptomics MS47 13 - UNIBO 48 transcriptomics analysis analysis finished done Microbiota analyses Table with statistic of MS48 13 - UNIBO 48 finished microbiota analysis done

Page 24 of 71 Work package number 9 WP2 Lead beneficiary 10 1 - KIS Work package title Performance and management of local pig breeds in their production systems Start month 1 End month 48

Objectives

• Evaluate productivity of different local pig breeds held in production systems with varying intensity and geo-climatic conditions • Assess environmental sustainability of production systems of local pig breeds • Evaluate impact of feeding strategies, resources and management considering animal welfare, environmental impact and economic benefits • Determine nutritional requirements of local pig breeds to optimize the efficiency and reduce environmental impact • Study innovative feeding and management practices in view of productivity and product quality • Evaluate locally available feeding resources and agro-by-products in view of productivity and product quality • Gut microbiota composition as related to feeding and management (pilot study)

Description of work and role of partners

WP2 - Performance and management of local pig breeds in their production systems [Months: 1-48] KIS, KGZS-NM, IFIP, INRA, BESH, UNIZG, PFOS, AGRIS, ANAS, UNIFI, UNIBO, LUHS, IPVC, UEVORA, IAH, UNIBG, INIA, CSIC, CICYTEX, IRTA, AECERIBER The aim of WP2 is to assess data on productive traits (and other relevant data) for multi-aspect evaluation of pig production systems based on local pig breeds, and to acquire knowledge on pig nutritional requirements, use of locally available feeding resources and innovative feeding and management strategies. To reach the aim, the work will be organised in five tasks:

Task 2.1 Collection of data and samples for multicriteria evaluation of pig production systems and comparative analysis of productive traits in local pig breeds

Partners: KIS (1, leader), KGZS-NM (3), IFIP (4), INRA (5), BESH (6), UNIZG (7), PFOS (8), AGRIS (9), ANAS (10), UNIFI (11), UNIBO (13), LUHS(15) , IPVC (16), UEVORA (17), IAH (18), UNIBG (19), INIA (20), CSIC (21), CICYTEX (23), IRTA* (24), AECERIBER (25) Start date and duration: month 1-48 Linked deliverable(s): D2.1, D2.2, D2.3; D2.8 (linked with D1.13)

In task 2.1, we will carry out a collection of data (with a common design and methods) on productive traits of local pig breeds, as well as indicators of animal welfare, environmental impact and economic performance, and collection of DNA samples (to be analysed in WP1). Data will be collected in production systems of different intensity (indoor, outdoor, organic), traditional feeding resources and climates, with accent on untapped local breeds. Summary and analysis of data on productive traits, collected through survey in local pig breeds, will be performed using qualitative and quantitative statistical methods; available literature data will also be collected and reviewed in support of overall evaluation of local pig breeds (if applicable we will perform meta-analysis). There will be fifteen local breeds included in this task, data will be recorded in 30 to 150 animals per breed (depending on breeds and traits), for all available productive traits (reproductive, growth/fattening, carcass and meat quality). Intestinal (gut) microbiota composition (by profiling 16S rRNA gene diversity with massive parallel sequencing technology analysed in WP1) will be put in relation to the effects of local breeds, production systems and/or feeding. The intestinal content/faeces sampling will be performed in a restricted and controlled manner; same breed submitted to different production system or feeding, different breeds held in the same environmental conditions. Activities of WP2.1 are a key to the success of WP1, WP2, WP3 and WP4, as they will furnish the data for samples for genetic assessments in WP1, products for the evaluation in WP3 and WP4 and meat quality measurements and analyses, as well as indicators of animal welfare, and data to assess environmental impact and economic performance (WP2).

Participants and roles: KIS and CSIC are responsible for coordination, protocol/design for data collection, overall compilation and analysis of data. All the partners are responsible to carry out field surveys with data collection and sample collection on their local pig breed(s).

Page 25 of 71 Third parties: it should be noted that breeders associations are involved in this task, as third parties to partners KGZS- NM, BESH, UNIZG, PFOS, UNIFI, LUHS, IPVC, UEVORA, IAH, INIA to support/facilitate collection of data and information on their respective local breeds. Partner IRTA will subcontract The Majorcan Black Pig Breeders Association to collaborate on data collection.

Task 2.2 The impact of production system on productivity, welfare and environment

Partners: INRA (leader, 5), KIS (1), UNIBO (13) Start date and duration: month 5-48 Linked deliverable(s): D2.7; D2.9

The impact of feeding strategies, resources and animal management practices studied within WP2 will be assessed regarding the issues such as animal welfare, environmental impact and economic performance, using previously developed tools (Welfare Quality, Q-Porkchains). Life-cycle analysis (LCA) of selected pig production systems of local pig breeds with varying intensity (indoor, outdoor, organic) will be performed. “Improved” LCA could be undertaken including the uncertainty of average data (e.g. climate variations) using dynamic models. Multicriteria analysis of the different production systems, feeding strategies, resources and practices will be completed using data from other tasks of WP2 as well as literature data and the expert knowledge of the partners. Task 2.2 will be in close complementarity with tasks 4.1 and 4.2. as task 2.2 will perform multicriteria analysis of systems, strategies and practices from a technical point of view (e.g. evaluation of impact on climate change – CO2 emissions in kg CO2-e/kg pig), and tasks 4.1 and 4.2 will use some common data with task 2.2 to perform the evaluation with a cost-benefit approach, i. e. from the economic point of view (e.g. evaluation of cost regarding impact on climate change in €/kg pig).

Participants and roles: INRA Coordination, analysis of selected production systems KIS Analysis of selected production systems in Krškopolje breed UNIBO Analysis of selected production systems in Mora Romagnola breed

Task 2.3 Nutritional requirements of growing pigs, and reproductive sows in selected local pig breeds in different environmental conditions.

Partners: CSIC (leader, 21), UNIFI (11), INRA (5) Start date and duration: month 1-38 Linked deliverable(s): D2.6

Contrary to thoroughly studied highly productive modern pig breeds, the nutritional requirements of autochthonous, local pig breeds are untapped. The objective is thus to improve the knowledge of nutritional requirements in local pig breeds which differentiate strongly in performance from modern pig breeds. Studies will be performed in growing pigs (in one trial including immunocastration), and in lactating sows. These studies will be performed on two local pig breeds, one more advanced (Ibérico) and one untapped (Cinta Senese). Complementary modelling studies to determine pig nutritional requirements at different physiological stages will be performed using InraPorc® software. Although nutritional requirements of Ibérico and Cinta Senese may not be directly transferred to other breeds, the results obtained on these two model breeds (chosen for infrastructure reasons) can improve know-how on nutrition of fatty type (local) pigs at different production stages.

Participants and roles: CSIC Coordination, nutritional requirements studies in lactating Ibérico sows, nutritional requirements of immunocastrated Ibérico males and females pigs. For experiment with lactating sows CSIC will subcontract a farm (facilities, assistance) INRA Modelling studies UNIFI Nutritional requirement studies on Cinta Senese breed Third parties: breeders association of Cinta Senese is involved in this task with partner UNIFI

Task 2.4 Innovative feeding strategies or management practices and their effects on production traits and product quality

Partners: UNIFI (leader, 11), KIS (1), KGZS-NM (3), BESH (6), PFOS (8), IPVC (16), UEVORA (17), IAH (18), CSIC (21), CICYTEX (23) Start date and duration: month 1-35 Linked deliverable(s): D2.4

Page 26 of 71 Experiments will be conducted in order to evaluate the effects of innovative practices in management and feeding on production traits (and product quality – interaction with WP3) including the aspects of animal welfare and health: • innovative feeding strategies (e.g. energy restriction, compensatory growth, germinated seeds) • innovative management practices involving animal welfare and health (e.g. organic vs. conventional, hoop barns, crossing two local breeds, immunocastration in view of the new European rules on pig castration applicable to the production of heavy local pigs). The final decision regarding the specific innovative strategy assessed by each partner will be decided at the start of the project (M2.1). Participants, roles, and breeds involved: UNIFI* Coordination; experiment on feeding strategies in Cinta Senese pigs KIS and KGZS-NM* Experiments on management practices in Krškopolje pig BESH* Experiment on management practices in Schwäbish-Hällisches pigs PFOS* Experiment on management practices in Crna slavonska pigs IPVC* Experiment on feeding/management strategies in Bisara pigs UEVORA* Experiment with crossing Bisara×Alentejana (Ribatejano) IAH* Experiment on management practices in Mangulica and Moravka pigs CSIC Experiment on management/feeding strategies in Ibérico pigs CICYTEX Experiment on management practices in Ibérico pigs Third parties: *partners that will carry out the activities in collaboration with third parties

Task 2.5 Local feeding resources rich in natural antioxidants and agro-by-products in the diets for local pig breeds

Partners: KIS (1, leader), KGZS-NM (3), IFIP (4), BESH (6), UNIZG (7), IPVC (16), IAH (18), INIA (20), CICYTEX (23) Start date and duration: M1-M36 Linked deliverable(s): D2.5

Experiments will be conducted in order to evaluate the feeding of local pig breeds with locally available resources rich in natural antioxidants (e.g. pasture, grass/hay, tannin rich wood resources or extracts) on product quality, with the emphasis on enhanced health benefits for consumer (interaction with WP3). Value of agro-by-products (e.g. olives, potatoes, milk/dairy by-products, fibre sources) for fattening local pig breeds will also be assessed in this task. Each partner involved in a task will conduct one trial including locally available feeding resources. As in some cases (partners) the potential resources are still under consideration, the final decision regarding the resource and trial designs will be decided at the start of the project (M2.1). Participants and roles: KIS Coordination; study in Krškopolje pig (roughage feeds grass/hay, dairy by products) KGZS-NM* study in Krškopolje pig (roughage feeds grass/hay, dairy by products) IFIP study in Gascon breed (pasture/forest resources) BESH* study in Schwäbish-Hällisches breed (grass/pasture) UNIZG* study in Turopolje breed (pasture/forest resources) IPVC* study in Bisara breed (local agro by products e.g. potatoes) IAH* study in Moravka and Mangulica breeds (supplementing a diet with tannin rich extract, interaction with WP1.7 – for pilot study on intestinal microbiota with two breeds in the same environmental conditions) INIA* study in Ibérico breed (alperujo - olive oil by product) CICYTEX study in Ibérico breed (energy restriction with fiber in premontanera period – interaction with WP2.4- animal welfare) Third parties: *partners that will carry out the activities in collaboration with third parties

Participation per Partner

Partner number and short name WP2 effort 1 - KIS 28.36 3 - KGZS-NM 2.99 4 - IFIP 2.07 5 - INRA 24.70

Page 27 of 71 Partner number and short name WP2 effort 6 - BESH 3.57 7 - UNIZG 5.77 POT 0.56 8 - PFOS 10.22 9 - AGRIS 0.93 10 - ANAS 0.65 11 - UNIFI 3.53 13 - UNIBO 11.80 15 - LUHS 1.28 LEFABA 0.29 16 - IPVC 5.90 ANCSUB 0.27 17 - UEVORA 1.36 ANCPA 0.27 18 - IAH 6.04 DUD 0.36 19 - UNIBG 2.90 20 - INIA 0.82 UNIEX 1.29 21 - CSIC 34.05 23 - CICYTEX 6.39 24 - IRTA 0.63 25 - AECERIBER 0.93 Total 157.93

List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Confidential, only Compilation of the for members of data on productive the consortium D2.1 1 - KIS Report 18 traits of local pig (including the breeds Commission Services) Compilation of the Confidential, only data for evaluation for members of D2.2 1 - KIS Report 19 of welfare, the consortium environmental (including the

Page 28 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 impact and Commission economic output Services) Confidential, only for members of Analytical review the consortium D2.3 of production traits 1 - KIS Report 30 (including the in local pig breeds Commission Services) Confidential, only Report with results for members of from the studies the consortium D2.4 on innovative 1 - KIS Report 35 (including the management Commission practices Services) Confidential, only for members of Report with results the consortium D2.5 of trials with local 1 - KIS Report 36 (including the feeding resources Commission Services) Confidential, only Report with results for members of from the studies the consortium D2.6 on nutritional 1 - KIS Report 38 (including the requirements of Commission local pig breeds Services) Sheets with description of the studied Confidential, only production systems for members of and innovative the consortium D2.7 strategies (average 1 - KIS Report 42 (including the and variability) Commission regarding the data Services) used as indicators for the tools used in task 2.2 Report on pilot study: microbiota Confidential, only composition for members of in relation to the consortium D2.8 1 - KIS Report 48 pig breed and (including the production system Commission – co-deliverable of Services) WP1.7 Report on the Confidential, only evaluation of for members of D2.9 1 - KIS Report 48 studied production the consortium systems and (including the

Page 29 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 innovative strategies in Commission regard to welfare, Services) environment, economics

Description of deliverables

D2.1 Compilation of the data on productive traits of local pig breeds (Month 18). D2.2 Compilation of the data for evaluation of welfare, environmental impact and economic output (Month 19) D2.3 Analytical review of production traits in local pig breeds (Month 30) D2.4 Report with results from the studies on innovative management practices (Month 35) D2.5 Report with results of trials with local feeding resources (Month 36) D2.6 Report with results from the studies on nutritional requirements of local pig breeds (Month 38) D2.7 Sheets with description of the studied production systems and innovative strategies (average and variability) regarding the data used as indicators for the tools used in task 2.2 (Month 42) D2.8 Report on pilot study: microbiota composition in relation to pig breed and production system – co-deliverable of WP1.7 (Month 48) D2.9 Report on the evaluation of studied production systems and innovative strategies in regard to welfare, environment, economics (Month 48) D2.1 : Compilation of the data on productive traits of local pig breeds [18] Compilation of the data on productive traits of local pig breeds D2.2 : Compilation of the data for evaluation of welfare, environmental impact and economic output [19] Compilation of the data for evaluation of welfare, environmental impact and economic output D2.3 : Analytical review of production traits in local pig breeds [30] Analytical review of production traits in local pig breeds D2.4 : Report with results from the studies on innovative management practices [35] Report with results from the studies on innovative management practices D2.5 : Report with results of trials with local feeding resources [36] Report with results of trials with local feeding resources D2.6 : Report with results from the studies on nutritional requirements of local pig breeds [38] Report with results from the studies on nutritional requirements of local pig breeds D2.7 : Sheets with description of the studied production systems and innovative strategies (average and variability) regarding the data used as indicators for the tools used in task 2.2 [42] Sheets with description of the studied production systems and innovative strategies (average and variability) regarding the data used as indicators for the tools used in task 2.2 D2.8 : Report on pilot study: microbiota composition in relation to pig breed and production system – co-deliverable of WP1.7 [48] Report on pilot study: microbiota composition in relation to pig breed and production system – co-deliverable of WP1.7 D2.9 : Report on the evaluation of studied production systems and innovative strategies in regard to welfare, environment, economics [48] Report on the evaluation of studied production systems and innovative strategies in regard to welfare, environment, economics

Page 30 of 71 Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Experiments Minutes of meeting confirmed, scheduled MS2 1 - KIS 2 and/or email exchange; and protocols validated protocols validated Survey questionnaire Minutes of meeting for 2.1 prepared, MS3 1 - KIS 3 and/or email exchange; harmonised and Questionnaire document confirmed. Pig production systems selected for Minutes of meeting MS5 task 2.2 and data 1 - KIS 6 and/or email exchange; collection protocols validated protocols prepared Notification of WP leader Field visits and with delivery of data; MS16 1 - KIS 15 surveys completed DNA samples shipped to WP1 partners Harmonised protocols for LCA and utilisation of Q- Minutes of meeting PorkChains and and/or email exchange; MS19 1 - KIS 18 Welfare Quality® validated protocols and tools and associated calculation sheets calculation sheets prepared Compilation of references on environmental impacts and/or List of references; concise MS24 production processes 1 - KIS 24 overview of key data for feed ingredients prepared used in the different pig production systems Notification of WP leader Experiments in tasks and report; products for MS32 1 - KIS 30 2.4 and 2.5 finished WP3 processed; tissue samples shipped Notification of WP leader Experiments in task MS33 1 - KIS 32 and report; tissue samples 2.3 finished shipped

Page 31 of 71 Work package number 9 WP3 Lead beneficiary 10 5 - INRA Work package title Traditional and new high quality products with regional identity Start month 1 End month 48

Objectives

• Characterize intrinsic quality, health benefits of a variety of pork products from diverse European local pig breed production systems • To introduce innovations in traditional pork products with the aim to develop new or re-discover products of high intrinsic quality and health benefits and expand market share • Identify links between product (typical) quality with local pig breed, feeding resources, management, processing techniques, “know-how” and cultural heritage • Evaluate consumer acceptability of traditional high quality pork products and innovative traditional pork products from untapped local pig breeds in different market areas • Implement database on carcass and meat quality traits to sustain further development of breeding programmes in local breeds

Description of work and role of partners

WP3 - Traditional and new high quality products with regional identity [Months: 1-48] INRA, KIS, IFIP, BESH, UNIZG, PFOS, UNIFI, UNIBO, LUHS, IPVC, UEVORA, IAH, UNIBG, INIA, CSIC, CREDA, CICYTEX, IRTA WP3 is based on a multi-actor approach gathering together partners from academia, real sector and end-users to evaluate the intrinsic quality attributes and consumer acceptability of a variety of European traditional pork products, according to pig breed, production system, animal feeding and management practices. The aim is to identify intrinsic quality and healthiness attributes that could be improved by animal management practices (e.g. feeding) or processing techniques. The ideas will be developed based on the knowledge and “know-how” of chain actors. New methods of products evaluation will be developed, techniques known from improved breeds which have not yet been applied on products of local pig breeds. Consumer acceptability studies will be made to link quality and acceptability of high quality pork products. Furthermore, database on carcass and meat quality traits will be designed and implemented in local breeds aiming at developing breeding programmes. To address these challenges, the WP is divided in 4 tasks.

Task 3.1 Development of a toolbox for the evaluation of sensory, healthy, technological and typical qualities of pork from local pig breeds

Partners: INRA (5; leader), KIS (1), IFIP (4), BESH* (6), UNIZG* (7), PFOS* (8), UNIFI* (11), UNIBO (13), LUHS* (15), IPVC* (16), UEVORA* (17), UNIBG * (19), INIA (20), IRTA (24) *Partners that will involve their third parties to carry out the activities Start date and duration: Month 1–15 Linked deliverable(s): D3.2

The aim is to develop a single common tool for the evaluation of sensory, healthy, technological (ability of raw meat to be processed into various products) and typical qualities of pork and pork products, as well as carcass composition from local pig breeds. For each quality trait, the toolbox will include common physico-chemical and biochemical indicators by pork product categories and specific threshold values for acceptability (i.e. minimum quality level required), according to local chains and products considered. »New« indicators recently developed and validated for the evaluation of carcass composition and meat quality traits (e.g. indicators of carcass composition determined by imaging, or biomarkers of pork eating quality determined by molecular techniques in previous international research projects) will be also considered. The toolbox will be developed based on the expertise and know-how of actors (research, development, pig breeders, producers, processing industries and consumers) of all diverse traditional pork chains involved in WP3.

Participants and roles: The task leader will organize meetings including all partners to share information and expertise, and will finalize the tool thanks to regular exchanges with partners.

Page 32 of 71 Task 3.2 Evaluation of sensory, healthy, technological and typical attributes of regional pork products from untapped breeds and new products issued from local chains

Partners: UNIFI (11; leader), KIS (1), IFIP (4), INRA (5), BESH* (6), UNIZG* (7), PFOS* (8), LUHS* (15), IPVC* (16), UEVORA* (17), IAH* (18), UNIBG* (19), INIA* (20), CSIC (21), CICYTEX (23), IRTA (24) *Partners that will involve their third parties to carry out the activities Start date and duration: month 8 – 48 Linked deliverable(s): D3.5, D3.6, D3.7, D3.8

The quality evaluation toolbox developed in task 3.1 will be implemented to determine sensory, healthy, technological and typical qualities of fresh meat and various processed pork products, and carcass composition from local pig breeds and traditional systems. In this task new methodologies and techniques for evaluation of carcass composition and meat quality traits will be considered including non-invasive (imaging: computed tomography, NIRS) and rapid molecular techniques that have been recently or are currently developed by partners within national or European research projects. In particular the fresh meat and pork products submitted to chemical analyses will be utilized to perform NIRS analysis in order to create a regression equation and implement the NIRS methodology as new method to predict meat quality, with special emphasis on prediction of lipid content and fatty acid composition (UNIFI, KIS).

Participants and roles: The task leader will coordinate the activities and especially ensure the collection of animals/pork samples and the finalization of analyses as scheduled by the partners involved, including harmonization of analytical methods of carcass and meat/processed products evaluation. A range of products from local pig breeds and production systems from diverse and contrasted European regions will be considered (Lithuania, Germany, France, Slovenia, Croatia, Serbia, Italy, Spain, Portugal) with special emphasis on untapped breeds or products. Average quality level and its variation within breed according to production system will be assessed. In this regards these breeds and products would be considered: • KIS: Carcass, fresh meat and processed products from Krškopolje pigs • INRA: Carcass, fresh meat and processed products (dry cured hams) from Gascon pigs. Use of biomarkers for the early post-mortem assessment/prediction of pork quality in various breeds studied in the project in close collaboration with other partners of task 3.2. • BESH*: Carcass, pork and processed products from Schwäbisch-Hällisches pigs • UNIZG*: Slavonian kulen produced from Crna slavonska pig breed (in collaboration with PFOS) and fresh meat and processed products from Turopolje pig breed. • UNIFI* : Carcass, dry-cured ham and salami (e.g. Capocollo) from Cinta Senese pigs • IAH – UNIBG*: Carcass, kulen (dry fermented sausages) and sremska kobasica produced from Mangulica and Moravka breeds. • LUHS*: Carcass and fresh meat from Lietuvos vietinės (Lithuanian Wattle) and senojo tipo Lietuvos baltosios (old type Lithuanian White) pigs • UEVORA*: Carcass, fresh meat and cured products from ‘Ribatejano’ (cross Bisara x Alentejana local breeds) pigs • IRTA: Fresh meat (Carpaccio, loin and Sobrasada) from Negre Mallorquí pigs *Partners that will involve their third parties to carry out the activities

Furthermore focus will be given to introduce innovations in traditional pork products in order to develop new products from these untapped breeds. The main aim of these innovations is to improve healthy attributes for consumers (reduced salt and fat content, improved fat composition and level of micronutrients and bioactive molecules to fit human nutritional requirements) or improve typicality of naturally fermented products using endogenous microflora without the use of starter cultures, and without impairing the sensory properties compared with the traditional products from same breed/production system. A second objective is to maintain and expand their market share. • UNIZG*: Development of dry-cured ham and dry fermented sausages of high healthy quality (reduced salt content, less smoking) from untapped Turopolje pig breed • UNIFI*: Naturally fermented salami and dry-cured ham with low salt content produced from Cinta Senese pigs • LUHS*: Hot smoked products with lower salt content from Lietuvos vietinės and senojo tipo Lietuvos baltosios pig breeds • IPVC*: Development and evaluation of quality of innovative pork products improving healthy (reduced salt) quality attributes in Bísara pigs • UEVORA*: Development of innovative pork products improving healthy (reduced salt) and typicality (use of endogenous microflora) qualities of Ribatejano pigs • IRTA: Development of low fat pork product (Sobrasada) and Carpaccio and Fresh loin with natural species from Negre Mallorquí pig breed *Partners that will involve their third parties to carry out the activities Page 33 of 71 Some of these innovative - traditional pork products will be tested in the Consumer test (Section 3.3) together with the traditional pork products.

Moreover, carcass composition and quality of fresh pork and pork products according to animal feeding strategy and management practices studied in WP2 (including alternatives methods to males and females surgical castration) will be characterized, thereby strengthening inter WP collaboration within the project. • KIS: Influence of feeding regime on carcass composition and meat quality of Krškopolje pigs • IFIP: (collaboration with INRA): Influence of feeding practices (pasture and forest resources) on carcass composition and quality of fresh meat and dry cured hams from Gascon pigs • BESH*: Influence of management strategy (conventional vs. organic production) and feeding resources (grass) on carcass composition and meat quality of Schwäbisch-Hällisches pigs • UNIZG*: Influence of local feeding resources on carcass composition and quality of meat from Turopolje pigs • PFOS*: Influence of animal management practices (housing system) on carcass composition and quality of traditional pork products from Crna slavonska pigs • UNIFI*: Influence of feeding strategy and growth rate of pigs on carcass composition and quality of meat of pork and pork products from Cinta Senese pigs • UNIBG* Influence of animal management practices on carcass composition and physical and biochemical properties of meat and pork products (kulen and sremska kobasica) from Mangulica and Moravka breeds • IPVC*: Carcass composition and quality of pork and pork products according animal management practices (including feeding regimen) in Bisara breed • INIA*: Influence of feeding resources (olive by-products) on carcass composition and quality of pork and pork products from Ibérico pigs • CSIC: Carcass composition and meat quality from immunocastrated Ibérico males and females pigs • CICYTEX: Carcass composition and quality of pork according to animal management (immunocastration) and feeding regimen (fiber sources) in Ibérico pigs *Partners that will involve their third parties to carry out the activities

Task 3.3 Consumer sensory studies

Partners: IRTA (24, leader), INRA (5), UNIZG (7), UNIFI (11), LUHS (15), CREDA (22) Start date and duration: month 12- 36 Linked deliverable(s): D3.1, D3.4

Sensory studies taking into account the objectives of the Call will be conducted, using a harmonized protocol that will be developed in this task, thus allowing comparison of results of sensory tests. Consumer sensory studies will be done in different EU member states and territorial market areas that can be interesting as a market for traditional pork products. A questionnaire developed in task 4.3 will be used to collect basic information about the respondents (age, sex, level of studies, habits pork consumption).

Participants and roles: The task leader will coordinate and design the Consumer studies testing Traditional pork products (TPP) and Innovative traditional pork products (ITPP) in 5 European locations from untapped breeds. A harmonized protocol will be developed for IRTA and translated for each partner to their own language for the consumer test. IRTA will be in charge of coordination of data collection, data entry, data cleaning and to conduct data analysis. Consumer sensory tests will be undertaken for fresh meat and TPP including ITPP that will be developed in task 3.2 in several breed/production systems. The consumers test will be conducted in divergent European city-market zones representing very different European regions: North (Lithuania), West (France), South (Spain and Italy) and East (Croatia), in cities that are identified as potential new markets for each local-autochthonous breed: Barcelona for the products of Negre Mallorquí pig breed, Paris for the Gascon breed, Milan for the Cinta Senese breed, Kaunas for the Lietuvos vietinės and senojo tipo Lietuvos baltosios breeds and Zagreb for the Turoplje breed. The test will have two objectives: 1) Acceptability of sensory properties between TPP and ITPP and 2) Acceptability of sensory properties between TPP and conventional ones. Triangular test to evaluate whether consumer notice from a sensorial point of view the differences in the new-innovative products in relation to traditional ones, as well as preference tests between traditional and conventional and liking will be assessed. Influence of information (traditional-conventional versus traditional-innovative) on the preferences of consumers could be also evaluated by testing (1) perceived preference (tasting with no information), (2) expected preference (stated preferences based only on information) and (3) actual preference (tasting with information). This information will be developed in WP4.3 and included in the tests. The meat and products from consumer studies will be provided by local companies and from the Institutes and universities related to each production system (each partner will be on charge of it). We will use the same questionnaire in Page 34 of 71 each country translated to the language of the region. Each partner will be on charge of recruitment of 120 consumers, to provide the products, perform the test and report to EU. Data from consumer studies will be analysed using appropriate statistical techniques by IRTA in coordination with CREDA. Results will be related to the Choice experiments and characterization of consumer attitudes and willingness to pay for the same products, which will be determined within WP4. These studies will improve the understanding of consumers’ acceptability of Traditional pork products including also innovations. The information obtained from this study may support companies to commercialize traditional and innovations in Traditional products in new markets.

Task 3.4 Database on carcass and meat quality traits for further development of breeding programmes in local breeds

Partners: IFIP (4, leader), KIS (1), BESH* (6), IPVC* (16) Start date and duration: month 1- 48 Linked deliverable(s): D3.3, D3.9 *Partners that will involve their third parties to carry out the activities; BESH will subcontract costs of herdbook data acquisition

In order to further implement strategies of breeding programmes including pork quality attributes in local pig breeds, a database on systematic determination of carcass and meat quality traits will be set up and implemented, together with experts and actors of the concerned (volunteer) breeding organizations. This will be conducted in close collaboration with WP1.

Participants and roles: The task leader will coordinate the activities from the organization of the initial consultation (experience and expectations sharing) to the development of a database which will be available for the different populations involved in the project. It is essential to link this database with existing herd-book databases in order to later calculate genetics parameters (heritabilities and correlations) which are necessary to define breeding objectives: the link will be done thanks to a unique animal identifier. Today the situation is very different from one country to another. None or very few carcass and meat quality traits are recorded in local Slovenian (Krškopolje) and French populations. In contrast, some meat quality traits are already implemented in the German Schwäbisch-Hällisches pig selection program. First of all, a state of the expectations of the breeding organizations will be done in order to define realistic requirements and functionalities of the database. The realisation will include the development of a carcass and meat quality traits database and of export and import programs in order to connect it with the different herd-books. This supposes to first define exchange formats. Each breeding organization could host its own data which should be accessible via Web if possible. We intend to use a common development platform, for example DotNet framework. Carcass and meat quality data that will be collected within WP1 and WP3 will be recorded in the database; special attention will be paid to data normalisation. Furthermore, the goal is to develop a progressive database allowing subsequent addition of new measurements.

Participation per Partner

Partner number and short name WP3 effort 1 - KIS 10.97 4 - IFIP 5.10 5 - INRA 15.70 6 - BESH 4.68 BESH AG 2.96 7 - UNIZG 7.73 8 - PFOS 3.63 11 - UNIFI 7.03 CONCS 0.46 13 - UNIBO 1.28

Page 35 of 71 Partner number and short name WP3 effort 15 - LUHS 5.74 16 - IPVC 19.31 17 - UEVORA 2.67 18 - IAH 2.04 19 - UNIBG 9.31 IMHT 2.67 20 - INIA 2.03 21 - CSIC 3.00 22 - CREDA 0.39 23 - CICYTEX 3.14 24 - IRTA 18.66 Total 128.50

List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Confidential, only Harmonized for members of protocol for the consortium D3.1 5 - INRA Other 14 consumer studies (including the in Europe (3.3) Commission Services) A toolbox for Confidential, only the evaluation for members of of sensory, the consortium D3.2 healthiness and 5 - INRA Report 15 (including the technological Commission quality of pork and Services) pork products. Specifications of the database and D3.3 5 - INRA Report Public 18 of the exchange formats Report on consumer acceptability of Traditional pork Confidential, only products (TPP) for members of from untapped the consortium D3.4 5 - INRA Report 36 breeds in relation (including the to Innovative Commission traditional pork Services) products (ITPP) in 5 European countries and Page 36 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 contrasted city-market zones, including comparison of TPP and conventional ones A report on the average and variation within breed/product of Confidential, only the sensory, healthy for members of and technological the consortium D3.5 qualities of pork 5 - INRA Report 48 (including the products from Commission various untapped Services) local breeds and production systems from contrasted European regions A report on the sensory, healthiness and Confidential, only technological for members of qualities of the consortium D3.6 innovative 5 - INRA Report 48 (including the traditional pork Commission products from local Services) breeds and aiming improved healthy quality A report on the influence of feeding strategy and animal Confidential, only management for members of practices on the consortium D3.7 5 - INRA Report 48 the carcass (including the composition and Commission sensory, healthy Services) and technological qualities of pork and pork products A report on Confidential, only prediction methods for members of of chemical the consortium D3.8 composition of 5 - INRA Report 48 (including the meat and pork Commission products by NIRs Services) analysis Database on Confidential, only D3.9 5 - INRA Report 48 systematic for members of Page 37 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 carcass and meat quality traits measurements the consortium in each local (including the breed aiming at Commission implementing Services) breeding programmes

Description of deliverables

D3.1 Harmonized protocol for consumer studies in Europe (3.3) (Month 14) D3.2 Toolbox for the evaluation of sensory, healthiness and technological quality of pork and pork products, including common physico-chemical and biochemical indicators by product categories and specific threshold values for acceptability, depending on local chains and products (3.1) (Month 15) D3.3 Specifications of the database and of the exchange formats (3.4) (Month 18) D3.4 A report on consumer acceptability of Traditional pork products from untapped breeds in relation to Innovative traditional pork products and to conventional products, in 5 European countries and contrasted city- market zones (3.3) (Month 36) D3.5 A report on the average and variation within breed/product of the sensory, healthy and technological qualities of pork products from various untapped local breeds and production systems from contrasted European regions (3.2) (Month 48) D3.6 A report on the sensory, healthy and technological qualities of innovative traditional pork products from local breeds (3.2) (Month 48) D3.7 A report on the influence of feeding strategy and animal management practices on carcass composition and sensory, healthy and technological qualities of pork and pork products (3.2) (Month 48) D3.8 A report on prediction methods of chemical composition of meat and pork products by NIRs analysis (3.2) (Month 48) D3.9 Database on systematic carcass and meat quality traits measurements in each local breed aiming at implementing breeding programmes (3.4) (Month 48) D3.1 : Harmonized protocol for consumer studies in Europe (3.3) [14] Harmonized protocol for consumer studies in Europe (3.3) D3.2 : A toolbox for the evaluation of sensory, healthiness and technological quality of pork and pork products. [15] A toolbox for the evaluation of sensory, healthiness and technological quality of pork and pork products. The toolbox will include common physico-chemical and biochemical indicators by pork product categories and specific threshold values for acceptability, depending on local chains and products D3.3 : Specifications of the database and of the exchange formats [18] Specifications of the database and of the exchange formats D3.4 : Report on consumer acceptability of Traditional pork products (TPP) from untapped breeds in relation to Innovative traditional pork products (ITPP) in 5 European countries and contrasted city-market zones, including comparison of TPP and conventional ones [36] A report on consumer acceptability of Traditional pork products (TPP) from untapped breeds in relation to Innovative traditional pork products (ITPP), in 5 European countries and contrasted city-market zones, including the comparison of TPP and conventional ones D3.5 : A report on the average and variation within breed/product of the sensory, healthy and technological qualities of pork products from various untapped local breeds and production systems from contrasted European regions [48] A report on the average and variation within breed/product of the sensory, healthy and technological qualities of pork products from various untapped local breeds and production systems from contrasted European regions D3.6 : A report on the sensory, healthiness and technological qualities of innovative traditional pork products from local breeds and aiming improved healthy quality [48] A report on the sensory, healthiness and technological qualities of innovative traditional pork products from local breeds and aiming improved healthy quality D3.7 : A report on the influence of feeding strategy and animal management practices on the carcass composition and sensory, healthy and technological qualities of pork and pork products [48] Page 38 of 71 A report on the influence of feeding strategy and animal management practices on the carcass composition and sensory, healthy and technological qualities of pork and pork products D3.8 : A report on prediction methods of chemical composition of meat and pork products by NIRs analysis [48] A report on prediction methods of chemical composition of meat and pork products by NIRs analysis D3.9 : Database on systematic carcass and meat quality traits measurements in each local breed aiming at implementing breeding programmes [48] Database on systematic carcass and meat quality traits measurements in each local breed aiming at implementing breeding programmes

Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) An agreed (among actors) list of indicators of Minutes of meeting and/ MS8 sensory, healthy and 5 - INRA 8 or email exchanges; recap technological quality file of pork and pork products Schedules for availability of carcasses, meat and products (including. Minutes of meeting and/ MS9 innovative products 5 - INRA 8 or email exchanges; recap and samples issued file from WP2) and submitted to quality evaluation Literature studies on innovative methodologies of meat quality analysis Minutes of meeting and/ MS11 5 - INRA 12 and list describing or email exchanges the new techniques selected for the project Agreement on Minutes of meeting and/ harmonized protocol MS12 5 - INRA 12 or email exchanges; recap for consumer studies file in Europe Agreement (among partners) on a framework of the database (list Minutes of meeting and/ of carcass and MS13 5 - INRA 12 or email exchanges; recap meat quality file indicators to be recorded) for further implementation to breeding programs

Page 39 of 71 Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Harmonized procedures for the determination of meat and pork product quality Minutes of meeting and/ MS15 including specific 5 - INRA 14 or email exchanges; recap thresholds values of file indicators of meat quality according to the chain/product considered Specifications of the database and of Minutes of meeting and/ MS20 the exchange format 5 - INRA 18 or email exchanges (export and import of data) Recap file of data Consumer sensory collected by partners MS25 5 - INRA 24 studies finalized involved in task 3.3 and sent to task leader Preliminary database Preliminary database MS39 to verify matching 5 - INRA 36 (file) produced by task with specifications leader Meat/pork products Collection of meat / samples collected, and MS40 products samples 5 - INRA 36 sent to WP3 partners (for finalized analyses undertaken by a unique partner) Analyses of meat and Data files per breed / MS45 processed products 5 - INRA 42 experiment completed Implementation and use of a final database on carcass Database produced by MS49 5 - INRA 48 and meat quality traits task leader measurements in local breeds

Page 40 of 71 Work package number 9 WP4 Lead beneficiary 10 24 - IRTA Work package title Consumer preferences and market research for sustainable pork chains Start month 1 End month 48

Objectives

This WP aims at analysing the socioeconomic impact of the suggested changes in WP1, 2 and 3 on specific production systems and Traditional products from local breeds along the food chain. This analysis will cover a cost-benefit analysis at farm and societal level, acceptability along the food chain and a consumer analysis to better understand their attitudes and opinions towards the traditional pork products (TPP) and innovative traditional pork products (ITPP) and to evaluate their preferences and willingness-to-pay for specific pork products in different European regions. Specific objectives: • Analyse costs and benefits (private and public) of production systems along the food chain based on local and untapped pig breeds. • Evaluate consumer attitudes and preferences, motives, willingness to pay for pork traditional pork products and innovative traditional pork products from local untapped pig breeds. • Evaluate marketing strategies (short chains and/or direct sale channels) for stimulating the sales of local pork products. Route Map for a New collective trademark for European products originating from local pig breeds. • To develop buying behaviour model related to traditional and innovative traditional products in order to understand consumer decision making process.

Description of work and role of partners

WP4 - Consumer preferences and market research for sustainable pork chains [Months: 1-48] IRTA, KIS, INRA, BESH, UNIZG, UNIFI, LUHS, IAH, UNIBG, CREDA The following tasks are scheduled to address the challenges of WP4:

Task 4.1 Cost benefit analysis at farm and product level considering the private component of benefits.

Partners involved: CREDA (leader, 22), IRTA (24), INRA (5), UNIFI (11), LUHS (15), IAH (18), UNIBG (19) Start date and duration: Month 1 - 36 Linked deliverable(s): D 4.1, D 4.6

This task focuses on the analysis of the Cost-Benefit Analysis at farm and product level considering the private component of benefits and costs, and identification of management practices to optimize economic performance. From one side, within each analyzed system the production costs are identified at farm level. These costs account for the use of several production inputs (feeding, veterinary services, medical treatments, labor force, assets depreciation and other production costs). On the other side, the production revenues are also calculated mainly on the basis of the total outputs sales. As a result, the net present value (NPV) is calculated and then discounted (using a specific discount rate for each case study).

Participants and roles: The role of each partner will be to gather economic data at farm level. A meeting will be prepared in Barcelona to explain which information should be collected and in which format. A standardized questionnaire will be designed in the local language of each case study. This task is tightly related to WP2 where information is gathered co-ordinately.

Task 4.2 Cost-benefit analysis for the society considering the public component of benefits.

Partners involved: CREDA (leader, 22), IRTA (24), INRA (5), UNIFI (11), LUHS (7), IAH (18), UNIBG(19) Start date and duration: Month 6- 42 Linked deliverable(s): D 4.2, D 4.4, D 4.5, D 4.10

Cost-Benefit Analysis for the society considering the public goods (PG) component of benefits is included. The implications of traditional pork chains in the socio-agricultural ecosystem such as environmental impact, landscape maintenance, tourism activities, contamination by organic waste, abandon of local farming activity, gender issues, availability of healthier products or revival and enhancement of local traditions are economically valuated. Agricultural activities rely on a suite of supporting and regulating public goods as inputs (e.g., soil fertility and pollination) to Page 41 of 71 provide mainly food as well as a range of accompanying but non-marketed public goods (such as preservation of natural resources (air, soil, water) and other cultural elements). Agriculture can also be responsible for ecosystem dis-services (e.g. nutrient losses, biodiversity loss). These flows of services and dis-services depend directly on how agro- ecosystems are managed. We can conclude that the PGs are a list of natural and cultural elements that should be protected/corrected or increased/decreased in order to correspond to contemporary societal demands. In this context, the identification and quantification of these PGs requires simple, reliable, widely shared tools to measure their provision and justify financial reward on a scientific basis. In our specific case, firstly we need to identify PGs typology in each case study. Second, the discrete choice modelling technique is used to estimate the economic value (positive or negative) of the identified PG. These values are further used to complete the private Cost-benefit Analysis calculated in Task 4.1. The final social NPV is then calculated. The discrete choice modelling approach involves the characterization of the object of study, in our case agricultural public goods, through a series of attributes that can be combined to create hypothetical scenarios that will be evaluated by the subject. Usually, the status quo (current levels of the various attributes) with zero additional cost is one of the first proposed scenario. Other scenarios are proposed, representing changes in the levels of one or more attributes which imply an improvement or deterioration over the status quo situation and involve an extra cost for the subject that, in most cases, is paid via taxation. The target population of the study comprises citizens above the age of 18 living in the region of each agricultural system. In doing so, we focus our attention on the local demand for this type of goods. The decision is based on the impossibility of determining a priori the geographical limits of the population that would be interested in the provision of such goods by this agricultural system. Furthermore, selecting non-residents increases the bias due to the embedding effect. A meeting will be held in Barcelona for designing and guiding the partners for the empirical application.

Participants and roles: Each partner will collect information about the value of the public goods in each case study using a structured questionnaire to apply the CE valuation method.

Task 4.3 Consumer Preferences and willingness to pay for Traditional pork products and New Traditional pork products.

Partners involved: IRTA (leader, 24), CREDA (22), KIS (1), INRA (5), UNIZG (7), UNIFI (11), LUHS (15). Start date and duration: Month 8- 36 Linked deliverable(s): D 4.3, D 4.7, D 4.8

Consumer preferences and willingness to pay (WTP) for Traditional pork products and new products. A range of acceptable prices for pork products from local pig breeds will be determined according to WTP (IRTA). This task will address consumer driven definition for the concept Traditional pork products (TPP), and Innovations in Traditional pork products (ITPP) from untapped breeds. Also the main attributes that are preferred by consumers in these traditional pork production systems, in relation to conventional ones will be compared across six European countries that represent 4 different European regions: North (Lithuania), South (Spain and Italy), East (Croatia and Slovenia) and West-Centre (France). We will study the purchase intention for choice pork traditional products and for the innovative pork traditional products in a cross cultural approach in these very different European regions. To achieve this, a non-hypothetical Choice experiment (experimental economic field) will be carried out, including a session of sensory test in each region and with 120 consumers (see also WP3, Task 3.3). A harmonized protocol will be elaborated and translated to each region language and will be developed during the choice experiments in the 6 countries that participate in this task. All the Choice will be done with the same protocol along the European regions. The impact of sensory perception and information will be analysed. The innovation aspect of the experiment is in the structure of the consumer test: 1. Questionnaire (Socio-demographics and practices about purchasing traditional pork products) 2. Choice experiment (theoretical) 3. Eating evaluation of the meat products, including information of the brand-breed and without it. 4. Second choice experiment and auction (option to buy the products) 5. Questionnaire about preferences Development of the choice experiment will be done by CREDA and IRTA. Description of attributes and levels used in the choice experiment for each production system as an example of the main motives that must be developed in this task (origin, price, ethical concern, convenience, natural content, health, sensory, familiarity...) will be done. Participants and roles: each partner will be responsible to conduct the Choice experiment, recruitment of consumers, organisation of the trial, data file and the report (Deliverable). Analysis of the data: The Statistical analysis of the choice test will be done by CREDA (Choice theoretical-Auction) and IRTA (Eating test).

Task 4.4 Marketing strategies and Road Map for the New collective European trademark for products originating from local pig breeds Page 42 of 71 Partners involved: CREDA (leader, 22), IRTA (24), KIS (1), INRA (5), UNIFI (11), LUHS (15), BESH (6) Start date and duration: Month 32- 45 Linked deliverable(s): D 4.9, D 4.11

Assessment of feasible marketing strategies to provide farmers and producers with guidelines for creating added value and matching market demands. Effective promotional and marketing strategies will be defined for the potential markets through developing the role of location in promoting more beneficial sales methods for farmers in non-traditional distribution channels (short distribution channels, direct sales). From the previous analysis of the cost and benefit structure at farm level, marketing and sales channels information is also gathered. Strength, Weakness, Opportunity, and Threat (SWOT) analysis is carried out to identify the critical point of the adopted marketing channels and to allow, for each case study, proposing their appropriate marketing strategies. Diversification with more added value products and direct marketing alternatives are developed to analyze viability and feasibility. Route Map for a Brand European label for Traditional pork products. This task will address also the analysis of the stakeholder using the focus group and deep interview procedure. The HORECA sector (Hotel, Restaurants and Catering) will be specifically analyzed as a target for a potential niche market. The Q methodology will be used to study the stakeholder "subjectivity" and their viewpoint for different marketing channel. In addition, the analytical Hierarchy Process (AHP) will be applied to assess main factors including the economic agent within each case study take into consideration in their decision making. Priorities are estimated and different marketing alternative are evaluated to set the most preferred one. IRTA and CREDA will propose a Route Map for New collective trademark for products originating from local pig breeds. Participants and roles: each case study will support the development of the focus groups sessions and the qualitative collection of the information through the deep interview. Each partner will be asked to select relevant stakeholders within two types of focus groups. The first encompassing the production side and the second deals with the retailers and point of purchase. A meeting with stakeholders will be done in Germany for discussion of a New collective trademark for European products originating from local pig breeds together with BESH (Stakeholders from the Representatives of the six Traditional Pork Chains studied will be invited) (participants in task 4.3).

Participation per Partner

Partner number and short name WP4 effort 1 - KIS 9.98 5 - INRA 9.60 6 - BESH 0.43 7 - UNIZG 3.60 11 - UNIFI 6.19 15 - LUHS 5.37 18 - IAH 3.00 19 - UNIBG 5.20 22 - CREDA 36.11 24 - IRTA 24.17 Total 103.65

Page 43 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Standardised Confidential, only protocol for for members of collecting data at the consortium D4.1 24 - IRTA Demonstrator 8 farm level for the (including the Six Production Commission systems involved Services) Updated and ungraded literature review on public Confidential, only good valuation for members of methods to the consortium D4.2 24 - IRTA Report 12 select the most (including the appropriate Commission methodological Services) approach to our cases studies Confidential, only Harmonised for members of protocol for Choice the consortium D4.3 experiment for the 24 - IRTA Demonstrator 14 (including the Six Production Commission systems involved Services) Standardized Confidential, only protocol for for members of collecting data at the consortium D4.4 24 - IRTA Demonstrator 18 citizen level for (including the the Six Production Commission systems involved Services) Results of the Confidential, only choice experiments for members of to estimate the total the consortium D4.5 value of the public 24 - IRTA Report 30 (including the goods produced in Commission each production Services) system Results of the Confidential, only CostBenefit for members of analysis and results the consortium D4.6 on heterogeneity 24 - IRTA Report 36 (including the between the Commission different analysed Services) regions Results of the Confidential, only Choice test taking for members of into account cross- the consortium D4.7 24 - IRTA Report 36 cultural differences (including the between the Commission European regions Services)

Page 44 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Results of the Confidential, only Choice test for members of for Traditional the consortium D4.8 24 - IRTA Report 36 products and (including the innovative Commission traditional products Services) Standardized survey with Confidential, only guidelines to be for members of discussed in the the consortium D4.9 focus groups for 24 - IRTA Demonstrator 36 (including the collecting data at Commission stakeholder for Services) the Six Production systems involved Results of the Confidential, only social CostBenefit for members of analysis by the consortium D4.10 24 - IRTA Report 42 including costs (including the and benefit at farm Commission level Services) Confidential, only Qualitative for members of results of the Q the consortium D4.11 24 - IRTA Report 45 methodology and (including the Focus groups Commission Services)

Description of deliverables

D4.1 Standardised protocol for collecting data at farm level for the Six Production systems involved (Month 8). D 4.2 Updated and ungraded literature review on public good valuation methods to select the most appropriate methodological approach to our cases studies (Month 12) D 4.3 Harmonised protocol for Choice experiment for the Six Production systems involved (Month 14). D 4.4 Standardized protocol for collecting data at citizen level for the Six Production systems involved (Month 18). D 4.5 Results of the choice experiments to estimate the total value of the public goods produced in each production system (Month 30). D 4.6 Results of the CBA analysis and results on heterogeneity between the different analysed regions (Month 36). D 4.7 Results of the Choice test taking into account cross-cultural differences between the European regions (Month 36). D 4.8 Results of the Choice test for Traditional products and innovative traditional products (month 36). D 4.9 Standardized survey with guidelines to be discussed in the focus groups for collecting data at stakeholder for the Six Production systems involved (Month 36). D 4.10 Results of the social CBA analysis by including costs and benefit at farm level (Month 42). D 4.11 Qualitative results of the Q methodology and Focus groups (Month 45) D4.1 : Standardised protocol for collecting data at farm level for the Six Production systems involved [8] Standardised protocol for collecting data at farm level for the Six Production systems involved D4.2 : Updated and ungraded literature review on public good valuation methods to select the most appropriate methodological approach to our cases studies [12] Updated and ungraded literature review on public good valuation methods to select the most appropriate methodological approach to our cases studies D4.3 : Harmonised protocol for Choice experiment for the Six Production systems involved [14] Harmonised protocol for Choice experiment for the Six Production systems involved Page 45 of 71 D4.4 : Standardized protocol for collecting data at citizen level for the Six Production systems involved [18] Standardized protocol for collecting data at citizen level for the Six Production systems involved D4.5 : Results of the choice experiments to estimate the total value of the public goods produced in each production system [30] Results of the choice experiments to estimate the total value of the public goods produced in each production system D4.6 : Results of the CostBenefit analysis and results on heterogeneity between the different analysed regions [36] Results of the CostBenefit analysis and results on heterogeneity between the different analysed regions D4.7 : Results of the Choice test taking into account cross-cultural differences between the European regions [36] Results of the Choice test taking into account cross-cultural differences between the European regions D4.8 : Results of the Choice test for Traditional products and innovative traditional products [36] Results of the Choice test for Traditional products and innovative traditional products D4.9 : Standardized survey with guidelines to be discussed in the focus groups for collecting data at stakeholder for the Six Production systems involved [36] Standardized survey with guidelines to be discussed in the focus groups for collecting data at stakeholder for the Six Production systems involved D4.10 : Results of the social CostBenefit analysis by including costs and benefit at farm level [42] Results of the social CostBenefit analysis by including costs and benefit at farm level D4.11 : Qualitative results of the Q methodology and Focus groups [45] Qualitative results of the Q methodology and Focus groups

Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Meeting for defining MS6 the protocol with 24 - IRTA 6 Minutes of the meeting involved partners Meeting for defining Harmonised protocol on Preferences- MS14 Choice, including all 24 - IRTA 12 Minutes of the meeting partners involved in tasks 3.3 and 4.3 at IRTA-Monells Meeting for defining the protocol with involved partner MS17 24 - IRTA 15 Minutes of the meeting based on the literature review and deliverable 4.2.1 Data collection Field notebook and MS26 at farm level and 24 - IRTA 24 analysis analysis complete Data collection at Field notebook and MS27 citizen level and 24 - IRTA 24 analysis analysis complete

Page 46 of 71 Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Data collection Field notebook and MS29 Preferences-Choice 24 - IRTA 26 analysis and analysis complete Meeting with stakeholders in Germany for the Route Map of MS41 24 - IRTA 36 Minutes of the meeting New collective trademark for products originating from local pig breeds Focus Groups and Recordings of focus MS44 deep interview with 24 - IRTA 40 groups and text derived stakeholders from interviews

Page 47 of 71 Work package number 9 WP5 Lead beneficiary 10 12 - CERTA Work package title Measures to maximise impact Start month 1 End month 48

Objectives

The objective of WP5 is to assure an efficient flow of information within and outside the project to achieve the impacts expected by the call and thus strengthen the innovation capacity of TREASURE network and the society. Measures will involve all levels, from dissemination, exploitation of results, to demonstration and communication, and will include all partners, and address all relevant stakeholders and general public. The aim is promoting the generation of active networking between representatives of the Traditional Meat Products Chain from different European Regions. More in detail, the objectives of WP5 include: • the creation of a platform for functional networking within/between regions and sectors related to local pig production systems • the valorisation of traditional pork chains through the development of a new collective trademark • to raise the awareness of consumer about the quality of products from local pig breeds (focus on sensory, healthy, technological and traceability/genuineness aspects) • the spreading of concepts about sustainability of local breeds/products, as economical sustainability (incentive to the local economy), social sustainability (recovery of long-time trades and activities, valorisation of know-how cultural heritage, revival of traditions), environmental sustainability (conservation of local ecosystem and symbiotic relations between territory and rearing, lower environmental impacts in production and supply chains, i.e. landscape preservation, lower emissions, less contamination, less organic waste, recovery from land abandon, healthier products) • the advertising of innovative products from local breeds (developed or re-discovered new products of high intrinsic quality) • the diffusion of good practices guidelines within farmers (animal management practices previously identified, improving efficiency and environmental impact, allowing economic performance optimization) and the results of experimentations (new management practices) • the promotion of direct sales from farmers

Description of work and role of partners

WP5 - Measures to maximise impact [Months: 1-48] CERTA, KIS, UL, KGZS-NM, IFIP, INRA, BESH, UNIZG, PFOS, AGRIS, ANAS, UNIFI, UNIBO, SSICA, LUHS, IPVC, UEVORA, IAH, UNIBG, INIA, CSIC, CREDA, CICYTEX, IRTA, AECERIBER WP5 activities concern dissemination, demonstration, use and promotion of TREASURE results and collective trademark and will be continuously developed during the whole duration of the project. WP5 is broken down into 4 tasks, involving the whole Consortium, as detailed below.

Task 5.1 Publications and organisation of meetings

Partners: CERTA (leader, 12), all partners Start date and duration: month 1-48 Linked deliverable(s): D5.1, D5.3, D5.8, D5.9

Task 5.1 foresees the dissemination of all the scientific and technical issues produced by the project by means of the publications to scientific and professional public and by means of organisation or participation at scientific conferences. Dissemination activities will also serve for promotional acts aiming to raise the awareness of the TREASURE collective trademark (developed in 5.2).

Publications Scientific and technical partners will be involved in the production of different kind of publications, such as scientific papers in peer-reviewed national and international journals, technical and good practice guidelines, manuals, newsletters, new protocols (especially arisen from WP2 and WP3 outputs). Moreover, for less-specialist public, journal articles (online and paper), books, brochures and leaflets, developed in different European languages, will be produced. The latter will also be useful as promotional material for TREASURE collective trademark (to be developed in Task 5.2). It is worth noting that a part of an issue of the journal “Animal” dedicated to TREASURE is foreseen, which

Page 48 of 71 will be managed by INRA. In regard to the scientific publications (which will be supervised by Scientific commitee) the consortium targets to publish the results of TREASURE in the journals with high impact factor; at least one peer- reviewed paper is forseen per each scientific task.

Participation to meetings or conference organisation Some international symposia, conferences and/or events are pinpointed, e.g. Congreso Mundial del Jamón, EAAP, ICoMST, Symposium on the Mediterranean Pig (to be held in Portugal, 2016), Meeting “Haute Gastronomie”, meeting and events organized by FESERPAE (European Autochthonous Extensive Porcine Breeds Federation), leaded by Elena Diéguez Garbayo, the manager of partner AECERIBER, workshops organized by ASICI (Inter-professional association of the Ibérico pig). For each congress, attendance procedures will be checked and from time to time new suitable congresses will be found.

Organization of meetings Another activity of Task 5.1 is the organization of meetings and events, during which a special emphasis will be given to the diffusion of TREASURE collective trademark. Followed are already identified events considered by the consortium (but not limited to): • MEETINGS FOR THE SCIENTIFIC COMMUNITY AND PORK CHAIN ACTORS (development, industries): an international congress organized by partner IAH (Serbia, October 2017/2019), a session at JRP (Journees de la Recherche Porcine) in France and session at ASD (Animal science days) conference. • WORKSHOPS for professional audience, presentation of new protocols, presentation of new methodologies for the management of the local pig populations, DNA based tools for animal and meat traceability and authentication, vaccination protocol for immunocastration etc.). E.g.: IRTA workshop to be held by on Traditional products of high quality from untapped local pig breeds, and focusing on the presentation of innovative marketing strategies, new distribution channels, theoretical sessions and taste experience.

Task 5.2 Development of a new collective trademark

Partners: CERTA (leader, 12), all partners Start date and duration: month 1-36 Linked deliverable(s): D5.2, D5.5

Task 5.2 foresees the development of a new collective trademark which is thought as an »umbrella« including all the European breeds and pork chain products involved in this project. The collective trademark will be an important exploitation instrument for the project foreseeing also the future involvement of other organizations, paying attention to the development of a »sustainable pork chain«, assessing voluntary quality standards and procedural guidelines. During TREASURE project, in a first phase (by month 24), specific guidelines concerning concept and rules for the collective trademark will be developed: we aim to organize a presentation of these during the Symposium on Mediterranean Pig to be held in Portugal in 2016. The following second phase will see the analysis of intellectual property protection, with the registration of the collective trademark as output. In close relationship with Task 5.1, 5.4, promotional activities for the collective trademark will be spread during various events organised by partners and via the web platform developed in Task 5.3.

Participant and roles: CERTA will have a leading and operative role in this task, both in coordinating the design of concept and rules of the collective trademark, and in its developing, registration and dissemination. All other partners will actively contribute in the design of concept and rules of the collective trademark.

Task 5.3 Web and media dissemination

Partners: CERTA (leader, 12), all partners Start date and duration: month1-48 Linked deliverable(s): D5.4

Task 5.3 is dedicated to web and media dissemination and comprises the development of pig breeds/products database and a web platform.

Database In the first phase (by month 28) a database of local pig breeds and related traditional products will be developed. Indeed, we will include breeds and products not limited to those involved in TREASURE, but involving also other European breeds/products.

Page 49 of 71 Web platform and media dissemination Web dissemination is a key point of TREASURE, aiming to reach several target users (general public, specialists, farmers, policy makers, associations, research organizations, etc.), both at local and at global level. Web dissemination will play a fundamental role in networking among regions and sectors. For this purpose, an integrated web platform will be created and maintained, in different European languages. It will include: the database produced in the previous phase, information about pigs’ traditional products, pages for farmers and breeders associations, information about the project development and a regular project newsletter, upcoming events, advertising pages, a page dedicated to new collective trademark and an e-commerce platform for selling farmers/breed associations products. In a first phase (by month 6) a showcase website will be released, in order to give a general view on the project and its objectives. It will also contain a secured-access sections, dedicated to project partners for managerial purposes (collaborative platform), to be used as a project repository and for document exchange and sharing. In the following months, the showcase website will be powered with add-ons in order to develop the whole structure of the integrated web platform described above. Moreover, each partner will contact local and national broadcasting stations (TV and radio), in order to get interviews and reports on TREASURE project, collective trademark and on specific project activities. Also social networks will be used to promote TREASURE project (Youtube, Facebook, ResearchGate, etc.).

Participant and roles: CERTA will have a leading and operative role in the development of the database and of the web platform, while all project partners may transfer project outputs and provide content to database and website platform. All the 25 partners will also actively collaborate in media dissemination, searching for contacts with local and national TV and radio.

Task 5.4 Demonstration and transfer of knowledge

Partners: CERTA (leader, 12), all partners Start date and duration: month 1-48 Linked deliverable(s): D5.1, D5.3, D5.5, D5.6, D5.7, D5.8, D5.9

Task 5.4 will be focused on one hand on demonstration and knowledge transfer of established successful pork chains to partners in territories where local breed pork chains are at start-up stage. On the other hand, Task 5.4 activity will foresee the transfer of knowledge from academia to farmers/industry, allowing a strong networking among public and private bodies. To this purpose, transfer of knowledge resulting from project outputs will be achieved, through the organization of ad- hoc events, training courses, and also a training school for technologies of dry cured meat products processing. Best practices arisen from transfer of knowledge experiences will be disseminated through the web platform. In this task some specific involvements of some partners have already be identified: SSICA will be responsible to organise the training school for technologies of high quality dry cured meat products. SSICA, thanks to its experience with QUBIC European project will play a strategic role in networking and transfer of knowledge towards the untapped breeds and their products. Training school with aim to (not exhaustive): improve the production of typical products, assure a competitive, sustainable and regular production, considering the peculiarities of regions (in terms of production processes and techniques), create a trans-regional training system attended by firms’ operators, craft associations, expert of meat products. AGRIS will develop of a good practice guide for Sarda breed rearing and feeding systems and meat processing and organise training course for Sarda pig breeders and meat processors; organisation of workshop with TREASURE project results UNIFI will organize, with the support of their associated third party, Consorzio di Tutela della Cinta Senese, "consumer orientation" courses, in order to appreciate the quality of the "non globalized" product PUBLIC PRESENTATIONS OF TREASURE (project, collective trademark, breeds, products) at local agro-food fairs, including “degustation of products from pig local breeds): e.g. a breeders’ meeting organized in Germany by partner BESH; at yearly contest event Kuleniada to be held in east Croatia (Slavonia region) partner PFOS will organize »Sausage Olympics«, at local agro-food fairs in various regions by INRA, IFIP, KIS, KGZS-NM, UL, UNIFI, AECERIBER.

Participants and roles: CERTA will have a coordination role, assuring with its know-how a smooth running of the activities organised in WP5. Special emphasis of its efforts will be on development and promotion of the TREASURE project and its collective trademark. CERTA as coordinator will also take care that TREASURE network remains active and functional by promoting exchanges of partners as speakers or demonstrators at locally organised events.

Each partner will have a responsibility to demonstrate results on: Page 50 of 71 • publishing scientific or technical papers, articles, leaflets, brochures etc. • active role in the project network (e.g. active participation at events organised by other partners i.e. as speaker, demonstrator; organising web forums etc., CERTA as coordinator will monitor and promote the exchanges). • organising workshops or events for local farmers, advisory service or industry in their respective countries (each partner at least two in project lifetime) by including other partners as speakers or demonstrators (CERTA as coordinator will monitor and promote the exchange of partners) • active role in development of the TREASURE collective trademark (support to CERTA) • Provide material, inputs for web platform organised and maintained by CERTA Besides publishing and other ways of dissemination of TREASURE results, the partners will deliver inputs for web dissemination, collective trademark development and will organise local or international events; following are listed some of the activities/partners already identified: UL + KIS co-organisers of session at ASD (Animal science days) conference KIS + UL + KZGS • presentation of TREASURE at yearly agro-food fair AGRA • demonstration activities aimed at Association of breeders of Krškopolje (DKP) and SMEs for meat processing • study visit to get acquainted with development and functioning of successful local pig production chain IFIP + INRA • a session at JRP (Journees de Recherche Porcine) in France • workshops during meetings of the Ligeral (herd book) members • presentation at Paris International Agricultural Show • trade fairs for high quality and local food in Toulouse (Sisqa) and in Rennes (Tablées du Rheu) BESH • organization of a breeders’ meeting (of local breeds) in Germany with presentation of TREASURE project; demonstration activities for farmers UNIZG • workshops for farmers/meat processors with the support of their associated third party, Plemenita opcina turopoljska (POT) PFOS • co-organisers of session at ASD (Animal science days) conference • organization of a contest of European fermented sausages, to be held in east Croatia during Kuleniada event • workshop for farmers/meat processors with the support of their associated third party, LETA AGRIS • development of a good practice guide on the Sarda breed rearing and feeding systems and meat processing; • organisation of training course for Sarda pig breeders and meat processors; • organisation of workshop with TREASURE project results UNIFI • with the support of their associated third party, Consorzio di Tutela della Cinta Senese, organise "consumer orientation" courses, in order to appreciate the quality of the "not globalized" product • presentation at agro-food fairs Cibus-Parma, Salone del gusto-Torino CERTA coordination of all activities in WP5, relevant brochures and leaflets for dissemination events, care for active project networking creation and maintenance of web platform, development of the collective trademark SSICA • training school for the technologies of high quality dry cured meat products LUHS • events for products demonstration, in collaboration with Lithuanian Endangered Farm Animal Breeders Association, its associated third party. IPVC • demonstration activities with the support of their associated third party, ANCSUB UEVORA • organisation of 8th International symposium on Mediterranean pig • demonstration activities with the support of their associated third party, ANCPA IAH + UNIBG • organization of an international scientific congress, foreseen in October 2017; • presentation of TREASURE at fair »Fair of ethnic food and drinks« in Belgrade, the largest Serbian traditional product manufacturer show, intended for the markets of Serbia and Southeast Europe Region, in which traditional pork products can be presented in a TREASURE stand (Belgrade, Serbia). This fair is an important event also from a commercial point Page 51 of 71 of view, being the possibility to get in contact with local and international buyers and potential investors. This activity will be supported by associated third parties, Association EcoVillage and Institute of Meat Hygiene and Technology of Belgrade IRTA • organization of a workshop (presentation of innovative marketing strategies, new distribution channels, theoretical sessions and taste experience) AECERIBER • TREASURE promotion at meeting and events organized by FESERPAE (European Autochthonous Extensive Porcine Breeds Federation), and workshops organized by ASICI (Inter-professional association of the Ibérico pig) and at local fairs of Pozoblanco, Salamanca and Zafra (Spain) • demonstration activities in their annual Training Workshops addressed to Ibérico pig producers, Third parties associated with main partners provide support to the organization of events.

Participation per Partner

Partner number and short name WP5 effort 1 - KIS 5.08 2 - UL 2.15 3 - KGZS-NM 1.62 4 - IFIP 0.83 5 - INRA 1.00 6 - BESH 0.64 BESH AG 0.54 7 - UNIZG 1.27 POT 0.26 8 - PFOS 2.96 9 - AGRIS 0.00 UNISS 1.14 10 - ANAS 0.65 11 - UNIFI 0.21 CONCS 0.43 12 - CERTA 39.29 13 - UNIBO 1.28 14 - SSICA 1.71 15 - LUHS 1.46 LEFABA 0.30 16 - IPVC 1.13 17 - UEVORA 1.17 ANCPA 0.27 18 - IAH 2.57 DUD 0.36 19 - UNIBG 3.47

Page 52 of 71 Partner number and short name WP5 effort IMHT 1.14 20 - INIA 0.75 21 - CSIC 0.28 22 - CREDA 1.14 23 - CICYTEX 0.33 24 - IRTA 1.81 25 - AECERIBER 2.64 Total 79.88

List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Review of year 1activities on D5.1 dissemination, 12 - CERTA Report Public 13 demonstration, communication Concept and rules D5.2 of the collective 12 - CERTA Report Public 24 trademark Review of year 2 activities on D5.3 dissemination, 12 - CERTA Report Public 25 demonstration, communication Local pig breeds D5.4 and products 12 - CERTA Report Public 28 database Certificate of registration D5.5 12 - CERTA Report Public 32 of collective trademark Report on Training D5.6 school for dry 12 - CERTA Report Public 34 cured products Good practice guide on Sarda D5.7 12 - CERTA Other Public 35 breed rearing and feeding systems Review of year 3 activities on D5.8 dissemination, 12 - CERTA Report Public 37 demonstration, communication

Page 53 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Review of year 4 activities on D5.9 dissemination, 12 - CERTA Report Public 48 demonstration, communication

Description of deliverables

D5.1 – Review of year 1 activities on dissemination, demonstration, communication (Month 13) D5.2 – Concept and rules of the collective trademark (Month 24) D5.3 – Review of year 2 activities on dissemination, demonstration, communication (Month 25) D5.4 – Local pig breeds and products database (Month 28) D5.5 – Certificate of registration of collective trademark (Month 32) D5.6 – Report on Training school for dry cured products (Month 34) D5.7 – Good practice guide on Sarda breed rearing and feeding systems (Month 35) D5.8 – Review of year 3 activities on dissemination, demonstration, communication (Month 37) D5.9 – Review of year 4 activities on dissemination, demonstration, communication (Month 48) D5.1 : Review of year 1activities on dissemination, demonstration, communication [13] Review of year 1activities on dissemination, demonstration, communication D5.2 : Concept and rules of the collective trademark [24] Concept and rules of the collective trademark D5.3 : Review of year 2 activities on dissemination, demonstration, communication [25] Review of year 2 activities on dissemination, demonstration, communication D5.4 : Local pig breeds and products database [28] Local pig breeds and products database D5.5 : Certificate of registration of collective trademark [32] Certificate of registration of collective trademark D5.6 : Report on Training school for dry cured products [34] Report on Training school for dry cured products D5.7 : Good practice guide on Sarda breed rearing and feeding systems [35] Good practice guide on Sarda breed rearing and feeding systems D5.8 : Review of year 3 activities on dissemination, demonstration, communication [37] Review of year 3 activities on dissemination, demonstration, communication D5.9 : Review of year 4 activities on dissemination, demonstration, communication [48] Review of year 4 activities on dissemination, demonstration, communication

Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Release of showcase MS7 12 - CERTA 6 Website ready for use website Concept and rules for Minutes of meetings MS21 collective trademark 12 - CERTA 20 and/or electronic agreed communication

Page 54 of 71 Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Database on breeds TREASURE website MS30 and products of 12 - CERTA 28 section available to access TREASURE prepared Collective trademark Document proving MS34 12 - CERTA 32 registered registration Training school for Report on activity and MS35 12 - CERTA 33 dry-cured products participants list Integrated web Records of testing and MS42 platform ready for 12 - CERTA 36 training end-users Resume of assessment by Route map for MS43 12 - CERTA 36 stakeholders at meeting in collective trade mark Germany (BESH)

Page 55 of 71 Work package number 9 WP6 Lead beneficiary 10 1 - KIS Work package title Management Start month 1 End month 48

Objectives

The responsibilities of Project coordinator, General Assembly and Management Office are described in §3.2 which also describes the project management. In the first 6 months a range of activities will focus on establishing the rules for efficient management of this project (project management collaborative platform, protocols and formats for reporting within the management structure and to the commission). The overall management plan designed by this project is aimed at achieving five main objectives: • Co-ordination of scientific and financial activities according to the work plan • Monitoring results to control their scientific quality with regard to the fulfilment of the planned objectives, quality of the result, timing, spent resources and worldwide state-of-the-art • Efficiency of the organization setup to support the project, with special attention paid to financial, logistics, information, coordination issues, quality and conformity to EC rules and procedures. • Prepare the financial and technical reports for the commission This WP contributes to the proper start of the project, ensures good practice in resources management, guarantees financial good practice at the managerial level and seeks for final agreement of all partners with regard to the management of resources and will lead to easy conduct and closure of the relevant project accounts in the participating organisations. It also fosters the legal involvement of the participants and participating organisations, enables legal decision making and elaborates the contractual and legal ending of the project.

Description of work and role of partners

WP6 - Management [Months: 1-48] KIS, INRA, UNIFI, CERTA, UNIBO, INIA, CSIC, CREDA, IRTA Task 6.1 Establishment of the Collaborative Platform

Start date and duration: Month 1-6 Involved partners: KIS (Leader, 1), CERTA (12), INRA (5), UNIFI (11), UNIBO (13), INIA(20), CSIC (21), CREDA (22), IRTA (24) Linked deliverable(s): D6.1

A Collaborative Platform (restricted to partners) will be setup within the TREASURE web platform, enabling dynamic online input of results for web dissemination, creation of approval workflows, private subspaces, synchronous facilities such as instant messaging, application sharing, virtual meetings, etc. This web based collaborative working space is designed for the management of the project and aims at facilitating information exchange between the partners and at providing access to the Project repository. Data management plan will be also prepared within this task.

Task 6.2 Establishment of Management plan, common protocols and formats for reporting

Start date and duration: Month 1-3 Involved partners: KIS (Leader, 1), CERTA (12), INRA (5), UNIFI (11), UNIBO (13), INIA(20), CSIC (21), CREDA (22), IRTA (24) Linked deliverable(s): D6.1

Management plan will be prepared at the start of the project to provide partners with practical guidelines for the management of the project. The plan will be prepared under supervision of the project coordinator and with support of WP leaders. It will be submitted to the General Assembly for the agreement at kick-off meeting of the project. Common protocols and formats for reporting within the management structure and to the commission (including the establishment of a common spreadsheet/database based invoice recording and financial reporting system) will be established. A workpackage scorecard will also be prepared to enable Project Coordinator and general Assembly the assessment of the TREASURE progress.

Page 56 of 71 Task 6.3: Scientific management

Start date and duration: month 1–48 Involved partners: KIS (Leader, 1), INRA (5), UNIFI (11), UNIBO (13), INIA(20), CSIC (21), CREDA (22), IRTA (24) Linked deliverable(s): D6.1, D6.2, D6.3

The scientific and technical management will cover (1) Co-ordination of the project activities in accordance to the work plan; (2) Monitoring of the achievements to control the scientific quality of the results with regard to the EC contract as well as to the state-of-the-art; (3) Risk management through appropriate mitigation measures and alternative approaches. The scientific and technical management includes the coordination work and the strategy development and decision making by the General Assembly. These strategic roles are presented in §3.2. All the interactions of the coordinator with the European Commission (meetings, consolidated reporting) are part of scientific and technical management actions.

Task 6.4 Administrative management

Start date and duration: month1-48 Involved partners: KIS (Leader, 1), CERTA (12) Linked deliverable(s): D6.1, D6.2, D6.3

This involves: • Organisation of the “Kick-off” meeting; • Organisation of the General Assembly meetings and follow up of their decisions; • Appraisal and monitoring of the project costs in order to predict, monitor, oversee and control the overall costs incurred and EC grants (globally, by project, by activity and by participant); • Administrative support to partners • Maintenance of the consortium agreement; • Day-to-day management. • Minutes of the management meetings (e.g. General Assembly) • Support to the activities of the Committees

Task 6.5 Reporting to EC

Start date and duration: month1-48 Involved partners: KIS (Leader, 1), all partners Linked deliverable(s): D6.1, D6.2, D6.3 • Follow-up of EC payments and distribution to partners; • Consolidated annual technical and financial reports (as specified in EU-contract); • Obtaining audit certificates from the partners.

Participation per Partner

Partner number and short name WP6 effort 1 - KIS 30.96 5 - INRA 1.30 11 - UNIFI 1.46 12 - CERTA 11.70 13 - UNIBO 2.43 20 - INIA 1.10 21 - CSIC 0.68 22 - CREDA 1.86 24 - IRTA 1.65 Total 53.14 Page 57 of 71 List of deliverables

Deliverable Dissemination level Due Date (in Deliverable Title Lead beneficiary Type 15 Number 14 16 months) 17 Organisation of the D6.1 kick-off meeting; 1 - KIS Report Public 1 Management plan Report formats for technical support D6.2 1 - KIS Report Public 3 and financial reports Confidential, only for members of Data management the consortium D6.3 plan; Collaborative 1 - KIS Other 6 (including the platform ready Commission Services) Confidential, only for members of TREASURE the consortium D6.4 1 - KIS Report 18 activity report 1 (including the Commission Services) Confidential, only for members of TREASURE the consortium D6.5 1 - KIS Report 36 activity report 2 (including the Commission Services) Confidential, only for members of TREASURE the consortium D6.6 1 - KIS Report 48 activity report 3 (including the Commission Services)

Description of deliverables

D6.1 Report on the activities performed in the period from month 1 to month 18 (Month 18) D6.2 Report on the activities performed in the period from month 19 to month 36 (Month 36) D6.3 Report on the activities performed in the period from month 37 to month 48 (Month 48) D6.1 : Organisation of the kick-off meeting; Management plan [1] Organisation of the kick-off meeting; Management plan D6.2 : Report formats for technical support and financial reports [3] Report formats for technical support and financial reports D6.3 : Data management plan; Collaborative platform ready [6] Data management plan will be prepared Collaborative platform ready for use D6.4 : TREASURE activity report 1 [18] Report on the activities performed in the period from month 19 to month 36 D6.5 : TREASURE activity report 2 [36] Report on the activities performed in the period from month 19 to month 36

Page 58 of 71 D6.6 : TREASURE activity report 3 [48] Report on the activities performed in the period from month 36 to month 48

Schedule of relevant Milestones

Due Date (in Milestone number 18 Milestone title Lead beneficiary Means of verification months) Kick-off meeting Minutes of meeting; completed, MS1 1 - KIS 1 management plan Management plan protocol prepared General Assembly MS10 1 - KIS 10 Minutes of meeting meeting 1 General Assembly MS22 1 - KIS 22 Minutes of meeting meetings 2 General Assembly MS36 1 - KIS 34 Minutes of meeting meetings 3 General Assembly MS46 1 - KIS 42 Minutes of meeting meetings 4

Page 59 of 71 1.3.4. WT4 List of milestones

Milestone Lead Due Date (in Milestone title WP number 9 Means of verification number 18 beneficiary months) 17 Kick-off meeting completed, Minutes of meeting; MS1 WP6 1 - KIS 1 Management plan management plan protocol prepared Experiments Minutes of meeting and/or confirmed, MS2 WP2 1 - KIS 2 email exchange; validated scheduled and protocols protocols validated Survey questionnaire Minutes of meeting MS3 for 2.1 prepared, WP2 1 - KIS 3 and/or email exchange; harmonised and Questionnaire document confirmed. Experiments confirmed, scheduled and Schedule of experiments MS4 data collection WP1 13 - UNIBO 4 and data collection protocol protocols prepared, questionnaire sent over Pig production systems selected Minutes of meeting and/or MS5 for task 2.2 and WP2 1 - KIS 6 email exchange; validated data collection protocols protocols prepared Meeting for defining the MS6 WP4 24 - IRTA 6 Minutes of the meeting protocol with involved partners Release of MS7 WP5 12 - CERTA 6 Website ready for use showcase website An agreed (among actors) list of indicators of Minutes of meeting and/or MS8 sensory, healthy WP3 5 - INRA 8 email exchanges; recap file and technological quality of pork and pork products Schedules for availability of carcasses, meat and products Minutes of meeting and/or MS9 (including. WP3 5 - INRA 8 email exchanges; recap file innovative products and samples issued from WP2) and

Page 60 of 71 Milestone Lead Due Date (in Milestone title WP number 9 Means of verification number 18 beneficiary months) 17 submitted to quality evaluation General Assembly MS10 WP6 1 - KIS 10 Minutes of meeting meeting 1 Literature studies on innovative methodologies of meat quality Minutes of meeting and/or MS11 WP3 5 - INRA 12 analysis and list email exchanges describing the new techniques selected for the project Agreement on harmonized Minutes of meeting and/or MS12 protocol for WP3 5 - INRA 12 email exchanges; recap file consumer studies in Europe Agreement (among partners) on a framework of the database (list of carcass and meat Minutes of meeting and/or MS13 WP3 5 - INRA 12 quality indicators email exchanges; recap file to be recorded) for further implementation to breeding programs Meeting for defining Harmonised protocol on Preferences- MS14 WP4 24 - IRTA 12 Minutes of the meeting Choice, including all partners involved in tasks 3.3 and 4.3 at IRTA-Monells Harmonized procedures for the determination of meat and pork product quality including specific Minutes of meeting and/or MS15 WP3 5 - INRA 14 thresholds values email exchanges; recap file of indicators of meat quality according to the chain/product considered Field visits and Notification of WP leader MS16 WP2 1 - KIS 15 surveys completed with delivery of data; DNA

Page 61 of 71 Milestone Lead Due Date (in Milestone title WP number 9 Means of verification number 18 beneficiary months) 17 samples shipped to WP1 partners Meeting for defining the protocol with involved partner MS17 WP4 24 - IRTA 15 Minutes of the meeting based on the literature review and deliverable 4.2.1 Table with number of Sample collection samples (and therefore MS18 for DNA studies WP1 13 - UNIBO 18 animals sampled) collected ended per population Harmonised protocols for LCA and utilisation of Minutes of meeting and/or Q-PorkChains email exchange; validated MS19 and Welfare WP2 1 - KIS 18 protocols and calculation Quality® tools sheets and associated calculation sheets prepared Specifications of the database and Minutes of meeting and/or MS20 of the exchange WP3 5 - INRA 18 email exchanges format (export and import of data) Concept and rules Minutes of meetings and/or MS21 for collective WP5 12 - CERTA 20 electronic communication trademark agreed General Assembly MS22 WP6 1 - KIS 22 Minutes of meeting meetings 2 Basic statistics on Genotyping of MS23 WP1 13 - UNIBO 24 segregation polymorphisms major genes and minor allele frequencies Compilation of references on environmental impacts and/ List of references; concise MS24 or production WP2 1 - KIS 24 overview of key data processes for feed prepared ingredients used in the different pig production systems Recap file of data collected Consumer sensory MS25 WP3 5 - INRA 24 by partners involved in task studies finalized 3.3 and sent to task leader

Page 62 of 71 Milestone Lead Due Date (in Milestone title WP number 9 Means of verification number 18 beneficiary months) 17 Data collection MS26 at farm level and WP4 24 - IRTA 24 Field notebook and analysis analysis complete Data collection at MS27 citizen level and WP4 24 - IRTA 24 Field notebook and analysis analysis complete Tissue collection Table with id of samples per MS28 WP1 13 - UNIBO 26 ended population Data collection Preferences- MS29 WP4 24 - IRTA 26 Field notebook and analysis Choice and analysis complete Database on breeds and products of TREASURE website MS30 WP5 12 - CERTA 28 TREASURE section available to access prepared Feacal samples Table with id of samples per MS31 WP1 13 - UNIBO 30 collection ended experiment Notification of WP leader Experiments in and report; products for MS32 tasks 2.4 and 2.5 WP2 1 - KIS 30 WP3 processed; tissue finished samples shipped Notification of WP leader Experiments in MS33 WP2 1 - KIS 32 and report; tissue samples task 2.3 finished shipped Collective Document proving MS34 trademark WP5 12 - CERTA 32 registration registered Training school for Report on activity and MS35 WP5 12 - CERTA 33 dry-cured products participants list General Assembly MS36 WP6 1 - KIS 34 Minutes of meeting meetings 3 Genotyping with MS37 WP1 13 - UNIBO 36 Table with results high density chip Genomics analysis Table with statistic of MS38 WP1 13 - UNIBO 36 finished genomic analysis done Preliminary database to verify Preliminary database (file) MS39 WP3 5 - INRA 36 matching with produced by task leader specifications Meat/pork products samples Collection of collected, and sent to WP3 MS40 meat /products WP3 5 - INRA 36 partners (for analyses samples finalized undertaken by a unique partner) Meeting with MS41 WP4 24 - IRTA 36 Minutes of the meeting stakeholders in Page 63 of 71 Milestone Lead Due Date (in Milestone title WP number 9 Means of verification number 18 beneficiary months) 17 Germany for the Route Map of New collective trademark for products originating from local pig breeds Integrated web Records of testing and MS42 platform ready for WP5 12 - CERTA 36 training end-users Route map for Resume of assessment by MS43 collective trade WP5 12 - CERTA 36 stakeholders at meeting in mark Germany (BESH) Focus Groups and Recordings of focus groups MS44 deep interview WP4 24 - IRTA 40 and text derived from with stakeholders interviews Analyses of meat and Data files per breed / MS45 WP3 5 - INRA 42 processed products experiment completed General Assembly MS46 WP6 1 - KIS 42 Minutes of meeting meetings 4 Table with statistic Transcriptomics MS47 WP1 13 - UNIBO 48 transcriptomics analysis analysis finished done Microbiota Table with statistic of MS48 WP1 13 - UNIBO 48 analyses finished microbiota analysis done Implementation and use of a final database Database produced by task MS49 on carcass and WP3 5 - INRA 48 leader meat quality traits measurements in local breeds

Page 64 of 71 1.3.5. WT5 Critical Implementation risks and mitigation actions

Proposed risk-mitigation Risk number Description of risk WP Number measures Close interaction with farmers via local breeders associations, partners or third parties in the project will make practically feasible the collection of required data. The sample Recruiting sufficient/ collection protocols will be adequate phenotypic carefully designed to assure data and samples for the success in the objectives R1 genetic, genomic and WP1 and close interaction with transcriptomic analyses of local representative will the regional autochthonous be set up to facilitate farm pig populations accessibility and sampling practical organization. Genotyping and sequencing protocols will be carefully designed to assure quality data to success in the objectives The close interaction with farmers via breeders Recruiting sufficient/ associations, partners or adequate data in field R2 WP2 third parties in the project surveys on farms of local will facilitate the partners pig breeds the collection of required data To prevent problems of supply or availability (seasonal) of natural Supply of local feeding resources or by-products at R3 resources for experiments WP2 the moment of performing foreseen the experiments, the final decision on resources will be taken at the beginning of the project Common methodology for life cycle inventories for feed ingredients as well as for the pig unit Evaluation of pig itself will be discussed production systems and and carefully prepared R4 innovative strategies in task WP2 (user guides) before the 2.2. is highly dependent on beginning of the task itself, the other tasks of WP2 relying on previous projects (Q-PorkChains, Casdar ECOALIM in France, Welfare Quality®) Collecting sufficient / A list of pork chains / R5 adequate meat / pork WP3 products to be sampled product samples from and analysed within WP3 Page 65 of 71 Proposed risk-mitigation Risk number Description of risk WP Number measures (including availability date) will be established at start of the task. Close interaction with local various traditional pork breeders associations, chains for meat quality partners or third parties analyses in the project will make practically feasible the collection of required fresh meat/processed products samples We will take advantage of the expertise of partners Agreement between (and their involved third partners on the common parties) to reach agreement indicators to be considered upon indicators. Moreover, to evaluate the sensory, for a given indicator, R6 WP3 healthy and technological specific threshold values for qualities of meat and pork acceptance/rejection will be products to develop the considered according to the toolbox pork chain and/or type of product (based on expertise of the concerned partners) Experiments will be General sanitary or disease conducted in farms problems on pigs especially displaying outdoor pens when reared outdoors equipped with fences (risk of specific diseases, complying with regulatory e.g. Brucellosis in a herd standards to reduce risk R7 WP2, WP3 leading to slaughtering as lower as possible of all pigs, pandemia of (especially for sows’ pens). African Swine fever in any In case of, a replacement of the selected breeds to with other traditional breeds perform the Choice test) involved in the project could be undertaken. In case of pig sanitary situation impaired in Specific sanitary risk: the country: extremely, due to recent detection of cancellation of activities African Swine Fewer in scheduled with Lithuanian wild boars in Lithuania, R8 WP3 pig breeds and LUHS a risk of pandemia in partner in tasks 3.2, 3.3 the porcine populations and 4.3 (Quality analyses of Lithuania has been and consumer studies on identified fresh meat and processed product) The quality of the focus Two-three additional groups can be affected by persons will be contacted R9 unforeseen circumstances WP4 in case their presence is which prevent a participant needed to attend

Page 66 of 71 Proposed risk-mitigation Risk number Description of risk WP Number measures Difficulties by some Additional training sessions R10 partners in the use of the WP5, WP6 for partners to be held by Collaborative Platform CeRTA Prompt replacement of the partner with a partner Partner withdrawal in the with similar expertise or R11 WP6 negotiation phase split of its tasks on the other partners of the same workpackage Support of partner legal Difficulty in Consortium offices in order to achieve R12 Agreement drawing up, WP6 the best agreement as soon especially for IP issues as possible

Page 67 of 71 1.3.6. WT6 Summary of project effort in person-months

Total Person/Months per WP1 WP2 WP3 WP4 WP5 WP6 Participant 1 - KIS 2.06 28.36 10.97 9.98 5.08 30.96 87.41 2 - UL 26.01 0 0 0 2.15 0 28.16 3 - KGZS-NM 0 2.99 0 0 1.62 0 4.61 · DKP 0 0 0 0 0 0 0 4 - IFIP 0.50 2.07 5.10 0 0.83 0 8.50 5 - INRA 11.60 24.70 15.70 9.60 1 1.30 63.90 6 - BESH 0.50 3.57 4.68 0.43 0.64 0 9.82 · BESH AG 0 0 2.96 0 0.54 0 3.50 7 - UNIZG 1.12 5.77 7.73 3.60 1.27 0 19.49 · POT 0 0.56 0 0 0.26 0 0.82 8 - PFOS 3.70 10.22 3.63 0 2.96 0 20.51 · LETA 0 0 0 0 0 0 0 9 - AGRIS 0.93 0.93 0 0 0 0 1.86 · UNISS 0 0 0 0 1.14 0 1.14 10 - ANAS 1.44 0.65 0 0 0.65 0 2.74 11 - UNIFI 8.05 3.53 7.03 6.19 0.21 1.46 26.47 · CONCS 0 0 0.46 0 0.43 0 0.89 12 - CERTA 0 0 0 0 39.29 11.70 50.99 13 - UNIBO 25.64 11.80 1.28 0 1.28 2.43 42.43 14 - SSICA 0 0 0 0 1.71 0 1.71 15 - LUHS 1.02 1.28 5.74 5.37 1.46 0 14.87 · LEFABA 0.15 0.29 0 0 0.30 0 0.74 16 - IPVC 0.71 5.90 19.31 0 1.13 0 27.05

Page 68 of 71 Total Person/Months per WP1 WP2 WP3 WP4 WP5 WP6 Participant · ANCSUB 0.13 0.27 0 0 0 0 0.40 17 - UEVORA 0.27 1.36 2.67 0 1.17 0 5.47 · ANCPA 0.13 0.27 0 0 0.27 0 0.67 18 - IAH 3.32 6.04 2.04 3 2.57 0 16.97 · DUD 0 0.36 0 0 0.36 0 0.72 19 - UNIBG 0 2.90 9.31 5.20 3.47 0 20.88 · IMHT 0 0 2.67 0 1.14 0 3.81 20 - INIA 10.94 0.82 2.03 0 0.75 1.10 15.64 · UNIEX 0 1.29 0 0 0 0 1.29 21 - CSIC 0.50 34.05 3 0 0.28 0.68 38.51 22 - CREDA 0 0 0.39 36.11 1.14 1.86 39.50 23 - CICYTEX 1.48 6.39 3.14 0 0.33 0 11.34 24 - IRTA 7.80 0.63 18.66 24.17 1.81 1.65 54.72 25 - AECERIBER 0 0.93 0 0 2.64 0 3.57 Total Person/Months 108 157.93 128.50 103.65 79.88 53.14 631.10

Page 69 of 71 1.3.7. WT7 Tentative schedule of project reviews

Review Tentative Planned venue Comments, if any number 19 timing of review RV1 21 Ljubljana RV2 39 Brussels RV3 48 Brussels

Page 70 of 71 1.4. Ethics Requirements

Ethics Issue Category Ethics Requirement Description - The applicant must confirm that the ethical standards and guidelines of Horizon2020 will be rigorously applied, regardless of the country NON-EU COUNTRIES in which the research is carried out. The applicant must provide details on the material which will be imported to/exported from EU and provide the adequate authorisations. - Copies of relevant authorisations (for breeders, suppliers, users, and facilities) for animal experiments must be forwarded to the REA. In case research protocols are not defined, general information must be ANIMALS provided on the nature of the experiments, the procedures to ensure the welfare of the animals, and how the Principle of the Three Rs will be applied. If applicable, copies of training certificates/personal licenses of the staff involved in animal experiments must be provided.

Page 71 of 71 1. Project number The project number has been assigned by the Commission as the unique identifier for your project. It cannot be changed. The project number should appear on each page of the grant agreement preparation documents (part A and part B) to prevent errors during its handling. 2. Project acronym Use the project acronym as given in the submitted proposal. It can generally not be changed. The same acronym should appear on each page of the grant agreement preparation documents (part A and part B) to prevent errors during its handling. 3. Project title Use the title (preferably no longer than 200 characters) as indicated in the submitted proposal. Minor corrections are possible if agreed during the preparation of the grant agreement. 4. Starting date Unless a specific (fixed) starting date is duly justified and agreed upon during the preparation of the Grant Agreement, the project will start on the first day of the month following the entry into force of the Grant Agreement (NB : entry into force = signature by the Commission). Please note that if a fixed starting date is used, you will be required to provide a written justification. 5. Duration Insert the duration of the project in full months. 6. Call (part) identifier The Call (part) identifier is the reference number given in the call or part of the call you were addressing, as indicated in the publication of the call in the Official Journal of the European Union. You have to use the identifier given by the Commission in the letter inviting to prepare the grant agreement. 7. Abstract 8. Project Entry Month The month at which the participant joined the consortium, month 1 marking the start date of the project, and all other start dates being relative to this start date. 9. Work Package number Work package number: WP1, WP2, WP3, ..., WPn 10. Lead beneficiary This must be one of the beneficiaries in the grant (not a third party) - Number of the beneficiary leading the work in this work package 11. Person-months per work package The total number of person-months allocated to each work package. 12. Start month Relative start date for the work in the specific work packages, month 1 marking the start date of the project, and all other start dates being relative to this start date. 13. End month Relative end date, month 1 marking the start date of the project, and all end dates being relative to this start date. 14. Deliverable number Deliverable numbers: D1 - Dn 15. Type Please indicate the type of the deliverable using one of the following codes: R Document, report DEM Demonstrator, pilot, prototype DEC Websites, patent fillings, videos, etc. OTHER 16. Dissemination level Please indicate the dissemination level using one of the following codes: PU Public CO Confidential, only for members of the consortium (including the Commission Services) CI Classified, as referred to in Commission Decision 2001/844/EC 17. Delivery date for Deliverable Month in which the deliverables will be available, month 1 marking the start date of the project, and all delivery dates being relative to this start date. 18. Milestone number Milestone number:MS1, MS2, ..., MSn 19. Review number Review number: RV1, RV2, ..., RVn 20. Installation Number Number progressively the installations of a same infrastructure. An installation is a part of an infrastructure that could be used independently from the rest. 21. Installation country Code of the country where the installation is located or IO if the access provider (the beneficiary or linked third party) is an international organization, an ERIC or a similar legal entity. 22. Type of access VA if virtual access, TA-uc if trans-national access with access costs declared on the basis of unit cost, TA-ac if trans-national access with access costs declared as actual costs, and TA-cb if trans-national access with access costs declared as a combination of actual costs and costs on the basis of unit cost. 23. Access costs Cost of the access provided under the project. For virtual access fill only the second column. For trans-national access fill one of the two columns or both according to the way access costs are declared. Trans-national access costs on the basis of unit cost will result from the unit cost by the quantity of access to be provided. TREASURE 634476 Ref. Ares(2015)585151 - 11/02/2015

TABLE OF CONTENTS

2.1. EXCELLENCE ...... 2

2.1.1 OBJECTIVES ...... 2 2.1.1.1. Rationale ...... 2 2.1.1.2. Aim and main objectives ...... 2 2.1.1.3. Relation to the work programme ...... 3 2.1.2. CONCEPT AND APPROACH ...... 4 2.1.2.1. Concept...... 4 2.1.2.2. The approach and methodology ...... 6 2.1.2.3. National or international research linked with the project ...... 7 2.1.2.4. Sex and gender issues ...... 9 2.1.3. AMBITION ...... 9 2.1.3.1. Characterisation of regional autochthonous pig populations in Europe (WP1) ...... 9 2.1.3.2. Management and performance of local pig breeds in their production systems (WP2) ...... 10 2.1.3.3. Traditional and new high quality pork products with regional identity (WP3) ...... 11 2.1.3.4. Consumer preferences and market research for sustainable pork chains (WP4) ...... 13 2.1.3.5. Innovation potential ...... 14 2.2. IMPACT ...... 14

2.2.1. EXPECTED IMPACTS ...... 14 2.2.1.1 Project contribution to expected impacts...... 14 2.2.1.2. Improving innovation capacity ...... 16 2.2.1.3. Improving other environmental and socially important impacts ...... 17 2.2.1.4. Barriers and obstacles to achieve the impacts ...... 17 2.2.2. MEASURES TO MAXIMISE IMPACT (WP5) ...... 17 2.2.2.1. Dissemination and exploitation of results ...... 17 2.2.2.2. Management of intellectual property rights (IPR) ...... 18 2.2.2.3. Communication activities ...... 19 2.2.2.4. Data management ...... 19 2.3. IMPLEMENTATION ...... 20

2.3.1. WORK PLAN – WORK PACKAGES, DELIVERABLES AND MILESTONE ...... 20 2.3.1.1. Brief presentation of the overall structure of the work plan ...... 20 2.3.1.2. Timing of different workpackages (Gantt) ...... 22 2.3.2. MANAGEMENT STRUCTURE AND PROCEDURES...... 23 2.3.2.1. Management structure description ...... 23 2.3.3. CONSORTIUM AS A WHOLE ...... 26 2.3.4. CAPACITY OF PARTICIPANTS AND LINKS TO THIRD PARTIES ...... 29 2.3.4.1 Participants ...... 29 2.3.4.2. Third parties involved ...... 78 2.3.5. PLANNED USE OF RESOURCES ...... 85 2.3.6. ETHICS AND SECURITY ...... 89 2.3.6.1 Ethics ...... 89

1 TREASURE 634476

2.1. EXCELLENCE 2.1.1 Objectives 2.1.1.1. Rationale Nowadays the public opinion in Europe is not very favourable to intensive pig production which faces animal welfare and environmental problems (Kanis et al, 2003). In many European regions, intensive pig production chains are no longer sustainable and are facing a decline in pig production and in self-provision1. It is estimated that one third of global cereal production is fed to animals, and pigs as omnivores are directly concurrent to human population for available crops (FAO, 2006). It can be expected that in the future, the sustainability of pig production systems will depend on the locally available feeding resources (Godfray et al., 2010), which vary from region to region, due to contrasted geo-agro-climatic conditions. Local pig breeds are adapted to Public opinion is not favourable to specific local environment and fed various locally available intensive pig production which is feedstuffs. So, besides their genetic merit for agro-biodiversity, confronted with environmental and they represent the basis for sustainable local pork chains, animal welfare issues. especially important for the regions where available arable land and/or cereal production is limited (Herrero et al., 2009). Contrary Local pig breeds and their respective to intensive pig production, local pig breeds and their respective production systems meet high criteria production systems are able to respond to the high criteria and and expectations of modern society expectations of modern society in regard to some environmental in regard to the environment, animal aspects (including forest management and landscape conservation; welfare and food quality. Lopez-Bote 1998, Edwards 2005, Dourmad and Casabianca 2013), animal welfare, food quality and healthiness (Verbeke et al., 2010). Traditional pork products represent often culinary heritage of the regions and have an excellent image by consumers due to typical quality attributes, which cannot be assured with pigs from conventional intensive husbandry (Bonneau & Lebret, 2010). Few cases of successful local breed chains exist in Europe and the vast majority of them remain an untapped potential. In terms of scientific substantiation, their performances and products are practically untapped and market potential of their products unexploited.

2.1.1.2. Aim and main objectives The aim of the project is to improve the knowledge, skills and competences necessary to develop existing and create new sustainable pork chains based on European local pig genetic resources (local breeds), which correspond to the highest consumer demands for quality and healthiness of pork products, and to the societal demands regarding animal welfare, environment and rural development.

Main objectives of the proposal are:

 Analyse the phenotypic and functional properties encompassed in local pig genetic resources (breeds), esp. untapped, and development of DNA tools for authentication, traceability, conservation and breeding programmes (activities under WP1).  Acquire knowledge on pig nutritional requirements, use of locally available feeding resources, innovative feeding and management strategies and environmental impacts of pig production systems (activities under WP2).  Build up quality/healthiness attributes of regional pork products in line with consumer demands including innovations in traditional products to meet healty requirements, and identify links between intrinsic qualities and production systems (activities under WP3).  Perform a cost/benefit analysis at different levels of the chain and for the society, and research of market potential and strategies for regional high quality products (activities under WP4).

1 http://www.pig333.com/pig-production-data/ (accessed June,1 2014)

2 TREASURE 634476

 Transfer/exchange of knowledge and expertise among partners, dissemination to end-users and general public. Creation of functional networks within and between different regions or sectors and the creation of a new (umbrella) collective trademark for meat and products from local pig breeds (activities under WP5).

2.1.1.3. Relation to the work programme The inherent value of European local pig breeds and productions systems represent not only the exceptional agricultural biodiversity but are also prerequisite for the unique regional high quality pork products. In many cases such products are part of culinary heritage and/or belong to a category of special quality (e.g. organic, PDO, PGI, TSG) for which there is increasing consumer demand. In order to improve the supply of the The key challenge is a markets with such pork products, activities on research, innovation and development of sustainable knowledge transfer are needed. Objectives shall be reached through pork chains in geo-agro- teaming of partners from research with real sector, advisory services climatically different regions (local technical institutes or Chambers of agriculture) and end-users of Europe by using the (farmers, consumers, policy makers) and by creation of functional biodiversity resources network of stakeholders in pork chains based on local pig breeds from encompassed in European North to South of Europe. By promoting these systems, societal demands local pig breeds. for local self-provision, short distribution chains, diversified agricultural activities, animal welfare and environment can be fulfilled. In a broader societal perspective better exploitation of locally available agro-resources (genetic and feed) contributes to the preservation of rural environment and landscape, viability of rural areas, as a foundation for economic growth of regional agro- food sector.

The link between the work programme and TREASURE activities is presented in Table 1.

Table 1: TREASURE relationship with the key demands of the call Key demands by the call: TREASURE proposes to study: Phenotypic and functional properties encompassed in genetic resources Enhance description, evaluation, of (local pig breeds) and development of DNA tools for authentication, local breeds traceability, conservation and breeding programmes. Enhance management and Knowledge on nutritional requirements, use of locally available feeding performance of local breeds along with their respective production resources, innovative feeding and management strategies and environmental impact of pig production systems. systems Deploy research, demonstration, Proposal consists of original research of basic principles/mechanisms, dissemination measures to develop innovative approaches in feeding and management, product quality sustainable production schemes assessment, consumer and market research, transfer of knowledge and (environmentally and economically). expertise. Relevant socio-economic dimension is attained with integrative costbenefit evaluation, which will include both the impact of preserving traditional systems in a region and of direct economic activity of the Relevant socio-economic dimension local breed pork chain stakeholders. Better exploitation of locally available agro-resources (feeding and genetic) contributes to the preservation of rural environment and landscape, viability of rural areas, as a foundation for economic growth of local agro-food sector. Cross-fertilising interactions will be assured with strong integration, from the initial phase of the project design, of partners from Include knowledge from the formal complementary sectors i.e. academia, professionals, real sector (SMEs), and informal sectors and other stakeholders (NGO, chambers of agriculture, end-users like consumers).

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Key demands by the call: TREASURE proposes to study:

Encourage networks within/between Through teaming in project activities (between and within the research, real sector, NGO) a network on local pig breeds from North to South regions of Europe will be created2. The focus is on development of sustainable pork chains through better exploitation of local pig breeds and locally available feed resources, Address the value chain for regional high quality products enhanced quality and healthiness of pork products meeting consumer expectations, assessment of market potential taking into consideration societal demands regarding the environment and welfare of pigs. Capture more systematically the Special emphasis will be on untapped pig breed resources, not yet value of diverse and so far structured in a sustainable food chain, which demands special efforts to untapped genetic resources expand existing knowledge and develop relevant food chains. Encourage broader use in breeding, Promoting benefits to the farmers and chain as a whole is a farming and food chain. prerequisite for broader use and self-sustainability of local pig breeds. Proposal addresses animal genetic resources - local pig breeds whose Address either livestock or crop genetic resources inherent value represents not only the exceptional agricultural biodiversity but is also precondition for unique pork products.

'Multi-actor approach’ with Partners from complementary sectors i.e. academia (universities, research institutes), professionals (farmers associations, advisory adequate involvement of the services), real sector (SMEs), and other stakeholders (NGO, chambers farming sector in activities of agriculture, end-users like consumers).

2.1.2. Concept and approach 2.1.2.1. Concept After 2nd World War agricultural systems in the western society undertook an “industrial strategy” of intensification, specialisation and large scale production. Having experienced negative side effects (social, ethical and environmental) of efficiency driven animal production systems the persistency or sustainability became important. “Sustainability of agricultural system” is based on holistic philosophy and denotes resource efficiency and functional integrity and concerns environment, genetic diversity, ethical and social aspects and economic value (Olesen et al., 2000). Capital driven animal husbandry systems led to abandoning of many pig breeds that were not profitable and became endangered. In the context of the (internationally binding) preservation of biotic TREASURE is built on the inherent value diversity the interest for autochthonous (local) breeds was revived encompassed in local pig breeds, their in the past thirty years. In spite of that, these breeds are still production systems and product largely supported by special policy mechanisms in order to qualities. The idea is a new paradigm ensure their preservation (Mendelsohn, 2003). This is one of the of pig production that meets societal critical points for the future because most of the local breeds are demands regarding environment, presently not managed in a secure way and depend upon genetic diversity, ethical and social financial support from the governments for preservation aspects and economic value. programmes. The best conservation strategy is the one that makes the breed self-sustaining without the use of external subsidies (Hiemstra, 2010). Theoretically the self-sustainable condition of a local pig breed should be reached by the exploitation (sale) of products characterized by an extra added value which in return assures breeding of a sufficient number of animals to have an adequate genetic diversity (Bozzi & Crovetti, 2013). Nevertheless this condition is seldom attained in the local pig breeds and the intervention of public bodies is often considered essential for preservation (Signorello & Pappalardo, 2003). A sustainable use of local breeds is possible with better exploitation of the image and reputation of local breeds (extrinsic cues) as well as quality attributes associated with their products (intrinsic cues). Studies show that for consumers, the importance of extrinsic cues for quality inference is increasing (Grunert, 2006), while the intrinsic cues are important as a limiting factor of the acceptability and repurchase. Therefore the activities to increase market potential and value of products are the key strategy in support of in situ conservation of the breed. The link between local breed, geographical area and the product quality (its intrinsic cues) are important for the

2 TREASURE network was initiated from Mediterranean Pig network

4 TREASURE 634476 success of commercial strategies as demonstrated by the examples in Spain or Portugal, where the Table 2: List of local pig breeds involved in the project traditional local pigs (Iberico in Spain, Alentejano in Portugal) are kept in special agro-silvo-pastoral Country Native name for Alternative name ecosystems (“dehesa” in Spain, "montado" in Portugal). pig/breed (found in the literature) As reviewed by Bozzi & Crovetti (2013), there is a DE Schwäbisch-Hällisches Schwäbisch-Hällisches constant increase of general interest and research activities in local pig breeds, but also a clear gap ES Ibérico Iberian between Ibérico and many other local breeds in ES Negre Mallorquí Black Majorcan Europe which remain untapped, characterised by small FR Gascon Gascon populations and reared in geographical areas where the availability of natural resources is not abundant. FR Basque Basque Self-support in feeds and nutrients is an important issue HR Crna slavonska Black Slavonian of sustainability, particularly delicate in pig production as pigs are direct concurrent to human population for HR Turopoljska Turopolje available crops. Exploitation of local pig breeds in IT Cinta Senese Cinta Senese their production systems, which are based on local IT Mora Romagnola Mora Romagnola feeding resources, providing products with attributes that are demanded and appreciated by the consumers IT Sarda Sarda is the basis for sustainable chains. The economic IT Apulo Calabrese Apulo Calabrese potential of local or traditional breeds and their production systems is far from being optimally IT Casertana Casertana exploited and represents a challenge and opportunity IT Nero Siciliano Nero Siciliano for the pig sector in the future. LT Lietuvos vietinės Lithuanian indigenous wattle The overall concept of the proposed project is a change in the paradigm of pig production systems LT Senojo tipo Lietuvos Old type Lithuanian White suggesting development of pork chains which are more baltosios extensive with better utilisation of locally available PT Bisaro/Bisara Bisaro feed resources and using local pig breeds. Besides their biodiversity merit, local pig breeds provide PT Alentejano/ Alentejano products with special sensory quality and regional Alentejana identity searched by consumers (Guerrero et al., RS Mangulica Mangalitsa 2010). With few exceptions, local pig breeds (Table 2) are unexploited and often endangered. Their RS Moravka Moravka preservation depends on the incomes from farming SI Krškopoljski/ Krškopolje activities, and by developing sustainable pork chains, Krškopoljska local pig breeds would no longer be endangered. However, as their productivity is lower, production chains can become sustainable only when their genetic potential is optimally used, production systems optimised and their products are viable on the market which shall be addressed in the present project.

To improve market potential of high quality products from local pig breeds it is essential to gain scientific proofs of their singularity, productivity and product quality, as well as develop traceability/authentication tools and to perform relevant assessments of local feed resources, management practices, impact for environment and consumer acceptability, and marketing strategies adapted to regional circumstances.

The main ideas involved are:  to characterize local pig breeds at phenotypic, genomic and functional level  to identify genome regions/genes for adaptive traits and resilience of local pig populations  to improve management of local pig populations with genetic tools  to develop DNA based tools for traceability and authentication  to study genotype×environment interaction (effect of feeding and management on animal phenotype (including gene expression level); gut microbiota characterisation)  to construct data collection of productive traits and evaluate productivity of local pig breeds held in production systems with varying intensity and geo-climatic conditions  to assess nutritional requirements of local pig breeds

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 to evaluate locally available feeding resources in local pig breeds  to evaluate innovative feeding/management practices in local pig breeds  to perform multi-criteria assessment, including life cycle analysis (LCA), of production systems of local pig breeds  to evaluate production systems of local pig breeds in view of the attributes of their products  to develop a toolbox adapted for the evaluation of high quality products with regional identity issue from the local pig breeds  to use the toolbox on a range of pork products from different local breeds and on innovative traditional pork products  to develop the registration of quality traits in breeding programme in local pig breeds  to perform cost/benefit analyses at farm as well as local chain levels  to perform consumer and market research related to these special agro-food systems  to transfer expertise and knowledge between partners  to create functional networks between academia and non-academia sector and between various regions, also by means of creating a new “umbrella” collective trade mark

The key challenge of TREASURE is to contribute to development of sustainable pork chains in geo-agro- climatically contrasted regions of Europe by promoting the biodiversity treasure encompassed in European local pig breeds. The majority of them are untapped and their potential for development of sustainable pork chains unused. The project is based on the holistic approach by covering all the aspects important for the sustainability of pork chain, from feeding resource, inherent genetic potential of local pig breeds, feeding and management strategies, product fabrication and evaluation till product marketing; consequently diversified competences of partners and trans-disciplinary strategy in research are needed.

In relation to Technology Readiness Level (TRL) according to the provisions of HORIZON 2020 – WORK PROGRAMME 2014-2015 Annexes G, the project proposal as a whole fulfils the level TRL8 (on a scale 1-9) given that it addresses sustainability of pork chains from the primary stage (basic principles observed) to the marketing of the products (system complete and qualified), however, depending on each specific activity, the idea addressed can be at different stages of TRL. The innovation component of the project is highly pertinent and is encompassed in the original research (e.g- functional genomics; metagenomics; metabolic studies on nutritional requirements), testing of innovative management practices in local pig breeds production systems, new or (re)discovered pork products with emphasis on health benefits, novel approaches in studies related to consumer attitudes and preferences, marketing strategies, and last but not least, knowledge transfer approach with a flow through cross-fertilisation between different sectors and regions.

2.1.2.2. The approach and methodology The project is built on the inherent value encompassed in local pig breeds, their production systems and product qualities with the idea of new paradigm of pig production that meets societal demands regarding environment, genetic diversity, ethical and social aspects and economic value. Many local pig breeds/systems and consequently partners are involved in the project, from academia and non-academia, public and real sector. It is important to note that proposal puts the emphasis on the untapped local pig breeds and products from different European regions. Nevertheless, the partners (both academia and real sector) from the regions with already established examples of successful pork chains will be engaged. On one hand, these chains have a need for further developments, and on the other hand, their experience and “know-how” in establishing functional and sustainable chains can be transferred to partners/regions where local breed pork chains are at start-up stage of development.

The overall strategy to address project objectives involves:  original research of basic principles and mechanisms related to genetic characterisation of local pig breeds covered in WP1 (including functional genomics and metagenomics as related to factors of feeding and management), nutritional requirements of local pig breeds, and life-cycle analysis (LCA) of local pig breeds production systems covered in WP2;  through field survey, experiments, and literature review build up a collection of data on productive traits and other parameters relevant for multi-criteria evaluation of local pig breeds and their production systems (activity of WP2)  through field trials assess actual/existing and innovation potential of local pig breeds’ productions systems (feeding resources, management); activity of WP2;

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 by use of previously developed tools (Q-PorkChains, Welfare Quality) perform a multi-aspect (environment, welfare, productivity) evaluation of production systems (activity of WP2);  cost/benefit analyses (for farm, product and society) covered by activities of WP4  development of toolbox for product quality assessment adapted to special products of local pig breeds (sensory and nutritional/healthiness) will be the activity of WP3;  application of toolbox for quality determination/characterisation of variety of products from local pig breeds including innovative-traditional products (activities of WP3)  consumer studies and market research related to various traditional products and innovations in traditional products from local pig breeds and relevant markets (activities of WP3 and WP4)  creation of functional network with a strong involvement, from the initial phase of the project, of partners from complementary formal and informal sectors i.e. academia (universities, research institutes), professionals (farmers associations, advisory services), real sector (SMEs), and other stakeholders (NGO, chambers of agriculture, end-users like consumers) which will be possible through multi-actor cooperation in activities of all WPs  the best possible exploitation of the results through the cross-fertilising interactions within project network assured by WP5

2.1.2.3. National or international research linked with the project TREASURE addresses the aspects of high relevance for the development of local agro-food sector as demonstrated by past and on-going national projects, EU research and rural development projects in which partners of the present proposal were included; at the same time demonstrating relevant competences of the consortium (Table 2, 3) to fulfil the tasks foreseen.

Table 2: Projects (national and EU financed) related to TREASURE in which partners are/were involved Short name Full title (programme) Duration Partners Expected synergies Truefood Traditional United Europe Food 2006- INRA Intrinsic quality of pork (FP6) 2010 KIS products with regional IRTA identity Q-PorkChains Improving the quality of 2007- INRA Tools for the evaluation pork/products for consumer: dvlp 2012 IFIP of sustainability of pig of an innovative, integrated and IRTA production systems. sustainable food pro-duction chain BESH Biomarkers of meat of high quality products matching quality consumer demands (FP7) QUBIC Animal Breeding: quality, 2009- INRA Enhancing added value biodiversity, innovation, 2011 SSICA of the products competitiveness (2007 - 2013 Programme MED) TICS Innovative techniques for 2014- UNIFI Product characterisation production of “Cuore di Spalla” 2015 -new product (National) FCT Portuguese traditional meat 2012- UEVORA product quality in local products: strategies to improve 2014 IPVC pig breeds safety and quality (National) SVE-07-2012 Quality of meat of farm and wild 2012- LUHS Production systems. animals from different rearing 2015 Intrinsic quality of pork systems in relation to healthy products with regional nutrition (National) identity Animal Change An Integration, mitigation, 2011- INRA LCA of productions adaptation options for sustainable 2015 systems based on local livestock production under climate pig breeds change (FP7) LowInputBreeds Development of integrated 2009- INRA Intrinsic quality of pork livestock breeding and 2014 UL products with regional management strategies to improve identity. Breeding infra- animal health, product quality and structure for local pig performance in European organic breeds. Added value of

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Short name Full title (programme) Duration Partners Expected synergies and ‘low input’ milk, meat and products egg production (FP7) AGBCS Application of molecular genetics 2012- PFOS DNA based in Crna slavonska pig breed 2014 characterization of local (National) pig breed IBMAPSEQ Application of genomics and 2012- INIA Genomic variability of massive parallel sequencing 2014 Ibérico pig strain methods to study genetic variants Guadyerbas that regulate growth, conformation and meat quality in pigs (National) FAIM Optimising and standardising non- 2012- KIS Methods for carcass and destructive imaging and 2015 IRTA meat quality evaluation spectroscopic methods to improve PFOS the determination of body IFIP composition and meat quality in LUHS farm animals (COST) UNIFI INRA AGROTUR KRAS – destination of 2010- KIS Intrinsic quality of pork autochthonous products (CBC 2014 products with regional Programme Italy-Slovenia 2007- identity 2013) AGER Advanced research in genomics 2011- UNIBO Genome-wide and processing technologies for 2014 association. Identification the Italian heavy pig production of molecular markers for chain (National) new phenotypes SELMOL Research and innovation in animal 2007- AGRIS Linkage association genetic improvement activities with 2010 UNIBO studies. Identification of molecular techniques to the genetic markers for pig competitiveness of the national selection livestock (National) COZAC Identification of the characteristics 2010- UNIBO Meat quality aspects in of fresh pork relevant to the 2013 pig populations transformation in PDO (National) AGL2011-25360 Comparative studies on amino 2012- CSIC Nutritional requirements acid and lipid metabolism of the 2014 of local pig breeds, Ibérico ... growth, nutrient product quality issues utilization and product quality (National) RTA2010- Animal welfare indicators of the 2011- CICYTEX Innovative practices - 00062-C02-02 Ibérico acorn pig production 2014 immunocastration system in montanera... ethological, performance, reproductive and carcass and meat quality implications (National) EADGENE European Animal Disease 2004- INRA Research tools (pig EADGENE_S Genomics Network of Excellence 2013 UL microarrays) for Animal Health and Food Safety (FP6)

Table 3: International patents in which partners are involved

Short name Full title Duration Partners Expected synergies RM2005 Tracer Natural Food 2005- UNIBO Patent for the traceability of food A000235 patent products valorize and exploit quality production of local pig populations PT103508 Biotechnol. procedure to 2007- UEVORA Patent on bacterial mixture culture obtain sausages with 2011 CSIC (Staphylococcus xylosus; Lactobacillus

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SP2281247 low biogenic amines sakei) to obtain traditional sausages content from Alentejano with low biogenic amines content and Iberico pig

2.1.2.4. Sex and gender issues Sex and gender issues that are important in the context of the research work refer to animal and consumer research in which these issues shall be properly addressed to control the potential effect on the experimental results, or to cover adequately the structure of the population involved in consumer research. E.g. in case of animal research project management structure foresees Ethical committee and necessary statements are provided in Section 5: Ethics and Security. Shortly all animals (pigs) will be kept and treated in accordance with standard farm practice and abattoir routines, respecting national and/or EU legislation. Samples will be collected by specialized technicians and performed with respect to relevant national and/or EU regulations (Council Directive 98/58/EC of 20 July 1998, concerning the protection of animals kept for farming purposes and successive amendment and correction (Regulation (EC) No 806/2003); Council Directive 2008/120/EC of 18 December 2008 laying down minimum standards for the protection of pigs.) Pigs of local breeds, the object of this project, are extensively farmed, they benefit from an environment corresponding to their needs for exercise and investigatory behavior, in agreement with their physiological and ethological needs. They are cared for from a sufficient number of adequately qualified staff with appropriate knowledge and professional competence. As regards the gender issue referring to the composition of the consortium, similar proportion of female and male researchers is involved in the project; however, coordination responsibilities have a higher representation of female researchers. In regard to other important issues such as employment opportunities, working hours, career development, the management of the project will apply a policy of equal opportunities for both genders, and of family-friendly arrangements in working hours. Within the project, a care will also be taken to promote employment and training opportunities for young people in early career.

2.1.3. Ambition 2.1.3.1. Characterisation of regional autochthonous pig populations in Europe (WP1) The ambition of WP1is to demonstrate singularity of local pig populations, particularly the untapped ones at different levels: phenotypic, genomic and transcriptomic. Genetic and genomic tools will be developed that are presently not yet available and which the farmers will be able to apply to better exploit local pig breeds. New breeding programmes and management of local pig populations will be possible based on genomic information to further advance the use of their uniqueness, production systems and products.

The knowledge of the structure and genetic connection among local pig populations is a prerequisite to their characterization. Neutral genetic markers are widely used to analyze population structures and to characterize the genetic relationships and diversity among breeds and populations as already applied to the main European pig breeds. However, in the autochthonous pig Advance beyond the state-of-the-art populations, which are very well adapted to specific local  genetic basis for phenotypic agro-climatic environments, these analyses are lacking or singularity and adaptive traits absent. Today, new molecular tools such as the PorcineSNP60  gene expression related to feeding, Beadchip are available to increase the knowledge of the management structure and relationships among porcine populations. The PorcineSNP60 Beadchip was developed using the four main  DNA markers for traceability, breeds primarily used in modern pig production and wild boar authentication samples, which make the chip a very valuable resource for most  new methodology for managing local types of pig genetic studies and an excellent tool for a variety pig breeds populations of future studies in pigs, providing thousands of useful SNPs  gut microbiota characterisation in markers. In local pig breeds, the allele frequency distribution local pig breeds of known major genes (causative polymorphisms) is not known; the genes regulating growth and appetite (e.g. IGF2, MC4R, LEPR), meat quality (e.g. RYR1, PRKAG3, CAST) or coat colour (e.g. MC1R, KIT) are particularly interesting to study in view of breed characterisation. Insulin-like growth factor 2 (IGF2) gene is one of the key genes that control muscle development. Moreover the mutation A3072G constitutes a causal mutation associated with growth and carcass composition related traits (Van Laere et al., 2003). Melanocortin receptor 4 (MC4R) and Leptin receptor (LEPR) genes play a key role in the control of feed intake, energy homeostasis,

9 TREASURE 634476 body weight regulation and fat mobilization in mammals. Polymorphisms in both genes in pigs such as MC4R c.1426 A>G and LEPRc.1987C>T have been associated with feed intake, growth and fatness (López-Buesa et al., 2013, Muñoz et al., 2011). Genes regulating meat quality traits: Ryanodine receptor 1 (RYR1) constitutes one of the few known genes affecting meat quality traits in pig. The mutation C1843T is responsible of the malignant hyperthermia syndrome in pigs (Fujii et al; 1991). Protein kinase, AMP-activated, gamma 3 gene (PRKAG3) plays a key role in glycogen storage. Different polymorphisms such as R200Q, T30N, G52S or V199I and in the porcine PRKAG3 gene are considered as candidate mutations affecting meat quality (Milan et al., 2000; Ryan et al., 2012). Calpastatin gene (CAST) codifies for a specific inhibitor of μ- and m-calpain (EC 3.4.22.17) proteases. Evidences indicate that calpastatin activity post-mortem is highly related to meat tenderness and other meat quality parameters. Several polymorphisms in this gene such as Ser66Asn, Arg249Lys, Ser638Arg, etc. have been identified and associated with meat quality in different porcine population analyzed (Ciobanu et al., 2004; Rohrer et al., 2012). Coat colour genes: Melanocortin receptor 1 (MC1R), also known as Extension locus, and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), also known as I locus, are considered the key genes of the coat colour variations. Moreover, in several livestock species, coat colour alleles are associated to breed origin traceability. In pig, at least six different MC1R alleles have been described, which correspond to four Extension alleles: wild type (E+), dominant black (Ed), spotted (Ep) and red (e) (Kijas et al., 1998; Giuffra et al., 2000). Furthermore, six different alleles, at least, have also been identified for the KIT gene (Kijas et al., 2001; Rubin et al., 2012), which correspond to four I alleles: recessive allele (i), black spots (Ip), belt (IBe) and dominant white (I) (Fontanesi et al., 2010; Andersson, 2011; Fernández et al., 2003). Gene expression is the most fundamental level at which the genotype of an organism gives rise to the phenotype. The discovery of key genes underlying the biological processes responsible of the distinctness productive and product quality related traits in local pig populations requires the use of genome-wide approaches such as microarrays and RNA sequencing. Gene expression will be studied in relation to factors applied on local pig breeds in WP2, e.g. immunocastration (a novel practice in particular in the case of local pig breeds) and use of special local feeding resources (e.g. rich in natural antioxidants). In pigs, the microbial ecosystem undergoes massive fluctuations after weaning (Konstantinov et al., 2004). Major quantitative and qualitative changes in the composition of the intestinal microbiota are due to the diet, environmental factors, and the host (Isaacson & Kim, 2012). It is well known that the host specificity (genotype effect) is an important factor shaping animal microbiota (Kostic et al., 2013). The ambition is to use gut microbiota characterisation as a “footprint” of production system. As such analysis would demand a project per se, a pilot study will be performed comparing one breed in different environments and two breeds in the same environmental conditions, in close collaboration with WP2. 2.1.3.2. Management and performance of local pig breeds in their production systems (WP2) Several WP2 goals span beyond the state-of-the-art. Unlike in modern breeds, there’s lack of knowledge about nutritional requirements of local pig breeds. There is also a a need to improve quality and healthiness of regional pork products by means of innovative feeding strategies (e.g. compensatory growth, restriction) or use of locally available feed resources. Local pig breeds are often kept outdoors and fattened to higher weight/age so alternatives to physical gonadectomy (e.g. immunocastration) are of interest in view of outdoor rearing and in view of the foreseen ban on castration. Integrative life cycle (LCA) analysis of production systems based on local pig breeds is needed. To understand how interaction of Advance beyond the state-of-the-art two ecosystems (animal, bacteria) could affect productive, evaluation of productivity in varying adaptive traits, health in local pig breeds, has not been studied  agro-geo-climatic conditions yet.  nutritional requirements Unlike modern breeds, the knowledge on growth and  management and feeding performance on local pig breeds is very limited. There have been few studies showing the specific metabolic characteristics  multi-criteria evaluation of pig of autochthonous compared to conventional pig breeds production systems including LCA (Fernández-Fígares et al., 2007) and their lower rates of growth  microbiota specificity related to and lean-tissue deposition (Barea et al., 2007). This particular local pig breeds and feeding metabolic profile implies that they also have specific nutritional requirements. Optimum protein/energy ratios for growing/fattening Ibérico pigs has been studied (Nieto et al., 2012), however, additional studies on various productive phases, and on other local pig breeds are demanded to acquire the knowledge on their nutritional requirements, prior to optimise growth and performance of autochthonous pigs. Therefore, studies concerning nutritional requirements (growing pigs of Cinta Senese, and lactating Ibérico sows) will be developed. An environmental factor such as ambient temperature will be also considered, as it is a key

10 TREASURE 634476 parameter affecting productivity. Complementary studies to evaluate nutritional requirements according to physical stage will be also performed using mathematical models and software already available. Multi- criteria evaluation of pig production systems, including integrative LCA analysis of production systems of local pig breeds is needed as there is a lack of information (Dourmad & Casabianca, 2013). Our ambition is to perform it on pig production systems involving traditional breeds and innovative strategies, in order to better understand the determinants involved in the sustainability of these systems. Indeed, most of the literature focused on the evaluation of averaged conventional systems (and to a much lower extent of organic systems) based on specialized breeds for LCA (Nguyen et al., 2011, Dolman et al., 2012) and few papers considered extensive systems and/or non specialised breeds for welfare assessment (Temple et al., 2012). Q- PorkChains project evaluated the sustainability of some pig production systems in Europe but did not investigate new/alternative approaches in feeding or management strategies as we plan to do. We will evaluate strategies and practices that are well suited for these systems (e.g. immunocastration), while accounting for the phenotypes of the traditional pig breeds. The integrative analysis foreseen will provide an inventory of emissions along the life cycle of production of local pigs, with evaluation of impacts such as climate change, eutrophication, sustainability, animal health and welfare, etc. very valuable for the assessment of local pig production systems. Strategic TREASURE objectives are related to improved quality and healthiness aspects of regional pork product by means of innovation in management and feeding strategies. Thus compensatory growth (i.e. energy restriction period followed by ad libitum re-feeding, see Lebret, 2008) will be studied in local pig breeds. This manoeuvring of growth may enhance muscle protein turnover and meat tenderness due to increased proteolytic activity. Other positive consequence for meat quality could be increased intramuscular fat (key for sensory quality). According to Lebret (2008) this desirable effect depends on the onset and duration of restriction and re-feeding periods. Less productive local breeds, which demand longer productive cycles, seem more suitable to adapt such strategies for enhancing intramuscular fat deposition than conventional pigs (Pugliese et al, 2013). The production based on local pig breeds systems is able to respond not only to the high expectations of consumers for high quality products but also to the increasing demands of modern society towards animal welfare issues. In-door housing (outdoor, organic) will be investigated, not only to evaluate performances and welfare but also to identify areas for future developments. Another welfare issue is pig castration, common practice (to prevent boar taint), presently being questioned in EU. Immunocastration [active immunization against gonadotropin- releasing hormone (GnRH)] has been proven as effective in prevention of sexual development and boar taint in conventional pigs (Batorek et al., 2012). It could be an alternative, particularly in extensive husbandry systems, for heavier, older pigs’ or in the case of gilts (gonadectomized to prevent unwanted oestrus or pregnancies in outdoor systems). Immunocastration affects also pig performance, carcass and meat quality (Batorek et al., 2012). Longer productive cycles in heavier and older pigs compared to standard pig makes the use of immunocastration more complex. As such, it is a subject that needs investigation in order to develop adapted vaccination protocols and evaluate consequences for performance and product quality. For Ibérico pigs it is known that the capacity for growth and lean tissue deposition rapidly decreases as the animal increases body weight (age) so the experiments relating immunocastration and nutritional requirements foreseen within TREASURE are particularly beyond the state of the art. 2.1.3.3. Traditional and new high quality pork products with regional identity (WP3) The main ambition of WP3 is to enhance quality and health benefits of traditional pork products (TPP) from local pig breeds, as their sustainability depends upon the successful marketing of their products. Specific tools for evaluation of these products (fresh and processed, traditional or innovative) will be used which is new to the local Advance beyond the state-of-the-art pig breeds. For the first time, in the context local pig breeds, product eating quality and healthiness will be associated to  develop toolbox for product quality consumer acceptability i.e. according to their actual place on evaluation the global market. The project also aims to overcome the  quality and healthiness attributes of problems related to the heterogeneity and difficulty in traditional and innovative traditional supplying the market with high but “constant” quality products. products from local pig breeds Thanks to consumer studies, the products, while remaining "niche products", could expand to a wider market.  consumer acceptability Pork and processed pork products from local breeds that did  links between production system and not undergo selection programs for improved growth rate and quality lean meat content, are mostly found to be of higher eating quality than meat and products from conventional genotypes (Bonneau and Lebret, 2010; Pugliese and Sirtori, 2012, for reviews). However the actual knowledge on the average intrinsic quality level of fresh meat and TPP and their variability in some European untapped native pig breeds is still scarce. Besides,

11 TREASURE 634476 quality of pork and pork products includes many dimensions: eating properties, healthiness benefits for consumers, technological properties, i.e. ability of raw meat/cuts to be processed into various typical products, ‘typicality’, and consumer acceptability (including willingness to pay), that are generally not all considered when evaluating quality of pork from native breeds and local chains. A multidimensional evaluation of quality of pork and pork products from various European untapped breeds and production systems/management practices, as well as of innovative ‘traditional’ products from local breeds will be assessed in the project. For this purpose, a quality toolbox including common quality indicators but specific threshold values for acceptability of carcasses/products according to the local chain/product considered will be developed. Recent methodologies that are currently developed in the meat science sector to evaluate meat quality will be widely used on these local breeds/traditional products e.g. near-infrared spectroscopy (Prevolnik et al., 2005; Weeranantanaphan et al., 2011; Vautier et al., 2013, molecular markers (Te Pas et al., 2011; Damon et al., 2013). Average level and variability of quality of meat and TPP will be assessed in at least 10 untapped pure breeds and in crosses of two native pig breeds. Carcass composition (determined by relative proportions of lean and fat tissues), as well as eating, nutritional, technological and ‘typical’ qualities of raw meat and processed products, resulting from genotype × environment interactions, will be studied in close collaboration with research activities conducted in WP2. Indeed the production system, e.g. the housing system together with the animal management practices: feeding strategy (e.g. restriction-re-alimentation leading to compensatory growth), nature of foodstuffs, and pig management practices (e.g. immunocastration,…) interfere with breed effect to determine the final quality of meat and pork products, especially in local breeds reared in specific systems (Lopez-Bote, 1998; Lebret, 2008; Pugliese et al., 2013). Actors of local breed/traditional pork chains have a real wish to describe precisely the impact of their management practices (especially feeding management with local foodstuffs) on animal performance and quality of meat and TPP, especially for chains involved in Protected Designation of Origin approaches. Therefore, the project aims to overcome the problems related to the heterogeneity and difficulty in supplying the market with high but “constant” quality products. Moreover, innovations aiming at improving the health benefits of traditional pork products (i.e. reduced salt or fat content, improved lipid or micronutrients composition, reduced smoking), or enhancing typicality of fermented products through use of endogenous microflora will be developed. There are many studies published in relation to the consumers’ preferences of pork and pork products from conventional production, but few results refer to consumers’ studies in relation to sensorial acceptability of TPP in Europe. The term “traditional” is not always understood in the same way in every country (Guerrero Asorey, 2012) and often, the differences in consumers’ acceptability between local/traditional products vs. conventional ones are unclear. The project will address this question in 5 contrasted European market zones. The role of information about local pig breed and production method that can influence consumers’ expectations (Karolyi, 2013) and therefore be used as a differentiation tool in marketing of TPP will be also assessed. Even if there is a large diversity of TPP among Europe, innovations to maintain or try to expand the market share and availability of TPP are needed. Four main dimensions emerged around the concept of innovation: novelty-change, variety, processing-technology, origin-ethnicity and convenience (Steptoe et al., 1995). However, no research on consumer test comparing acceptability of TPP and innovative-TPP (ITPP) in a harmonised way, including motives for purchasing TPP, have been done in Europe. Recent results by Haugaard et al., 2014) also highlighted the importance of considering consumer concerns in product innovations. Thus the project also aims at evaluating consumer’s attitudes toward innovation in TPP. Results on consumer sensory studies will be associated to consumer attitudes and preferences, motives, willingness to pay for pork traditional pork products from local untapped pig breeds evaluated in WP4. The project will thus provide important information for the society about the acceptability of these traditional and new products, in five contrasted European regions. Clear indications from the new markets will be explored. This information will be useful to expand availability of ITPP related to heritage and local origin, with direct profit for farmers and SME´s of the traditional chains. These data will be further integrated to implement a road map for a new collective trade mark of traditional pork products in Europe. Besides evaluation of intrinsic quality and consumer acceptability of TPP and ITPP, phenotypic data on carcass composition, fresh meat and pork products quality obtained within WP3 will be combined with high throughput genotypes produced in WP1 for genome-wide association studies, to try to identify genome regions and SNPs controlling carcass and meat traits in local breeds. Also, within the frame of the project, a database will be developed allowing the implementation of carcass and meat quality traits in breeding programmes. Indeed, this kind of tool, connected to herdbook databases, is still not available in several local breeds, but is essential to manage the large quantity of data that are needed to estimate genetic parameters and further estimate breeding values.

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Altogether, research activities developed within WP3 will be helpful to maintain and even improve diversity of genetic resources and traditional food products, thereby allowing enhance sustainability of local/traditional pork production chains. 2.1.3.4. Consumer preferences and market research for sustainable pork chains (WP4) WP4 tasks represent the first application that analyses different European pig production systems based on local pig breeds from production and societal viewpoint following the same methodology, identifying and quantifying both public and private goods and services in an integrative view. Furthermore, the analysis of consumers attitudes, preferences and willingness to pay by using hedonic attributes (label, format, price, origin, certified quality...) of pork products, together with consumers acceptance by using sensory attributes (visual aspect, colour, tenderness, juiciness, flavour...) will be the first application of this nature. Real choice experiments with real money will be applied to analyse consumers’ preferences through economically incentive method. In considering the private cost-benefit analysis (at farm level), we will be able to analyze viability of each production system in different European regions. Thus, a comparative analysis of technical and economic efficiency is highly relevant and may play a significant role in the farmer decisions. Adding to this approach the social aspects of cost and benefit, it allows analysing both approaches of each production system. Accounting for the economic value of public goods provided by agricultural systems is not new (Randall, 2002; Kallas et al., 2007). However, in our case studies we will follow an Advance beyond the state-of-the-art approach that is differentiated by two main characteristics: 1)  cost benefit analysis at farm and it will allows for a comparison of the estimates economic values society level between the different European systems which is a valuable  consumers’ motives for consumption information for decision makers that is, at present, not and purchase available and 2) the estimated values will be further used for the social cost benefit analysis following a benefit transferring  marketing strategies (short chains) procedure (Plummer, 2009) that will differentiated for each  route map for collective trade mark specific case study. On the other hand, analysing consumers’ attitudes and preferences will follow a novel approach that improves the internal and external validity of the choice experiment (CE) technique used in our cases studies (i.e. obtained from stated preference method from a survey). The hypothetical CE are usually weak in particular those that are not “incentive compatible” (Lusk and Schroeder, 2004). In Europe there is an increased interest in food in general and in pork products in particular related to a region or place of origin. This is also considered an important element for European culture, identity and heritage but also development and sustainability of rural areas (Committee of the Regions, 1996). Local pig breeds are often a basis for TPP, which consumers link to regional identity and higher quality (sensory). However, some TPP can have an image of a fatty product and with poor convenience. Guerrero et al., (2009) explored consumer-driven definition for the concept of traditional foods and innovation across six European countries. They found that traditional food products were similarly perceived across Europe, but some differences were detected for the concept of innovation. However, they did not concentrate in any specific food or category. So, in this proposal we would like to develop these objectives for traditional pork products (including innovation) from local pig breeds. Some work has been done on innovation in pork products from Negre Mallorquí (Gonzalez et al., 2013), but consumers preferences for this new product were not determined. Therefore, a similar methodology as used by Guerrero et al. (2009) based on qualitative studies and focus groups (Chambers and Smith, 1991) will be tested in different regions, allowing to assess willingness to pay of consumers for TPP and ITPP in specific new market zones. Innovation will be related to healthiness benefits and sensory quality. We expect differences across European regions in the “willingness to pay for direct sale” (Sanjuan et al., 2012) which will be very useful for all traditional pork chains. Familiarity, food naturalness are recognised as drivers for traditional food consumption, whereas convenience and health as barriers to traditional food consumption (Pieniak et al., 2009). In TREASURE we will study the motives for consumption of traditional pork and their effect on choice at the moment of purchasing and of eating in six traditional pork production systems related to untapped pork breeds (Gascon, Negre Mallorquí (Negre Mallorquí breed), Krškopolje, Turoplje, Lietuvos vietinės, Lithuanian white and Cinta Senese). In our approach participants will be first motivated in monetary terms to participate and later after analysing their sensorial acceptability of the product (Cambris et al., 2009) will be asked to buy it with real money during the experiment in the different case studies. This will allow estimating a non-biased value of the consumers’ real preference toward the TREASURE products. We have to know if the innovations related to improved product healthiness can be detected by consumers and if they have an effect on perceived sensory properties. Such research with a harmonised approach in different European regions has not been done

13 TREASURE 634476 before. We also plan to assess feasible marketing strategies and to check the view of traditional pork chains representatives, including producers, retailers, HORECA sector (representatives of restaurants) etc. We will put special emphasis on short distribution channels as a target for potential new market. It is expected that results will be helpful to authorities to design appropriate agricultural policy instruments in regard to traditional chains. Another output of the project will be the development of a Route Map for a new collective trademark as a window to new markets for products from local pig breeds. 2.1.3.5. Innovation potential OECD defines innovation as “implementation of new or significantly improved product (good, service, process, method, organisation)” and denotes the connection of knowledge with exploitation of market opportunities for new or improved product. López-Claros and Mata (2010) expands its meaning to “innovation is creative use of knowledge that allows Innovation potential of TREASURE is to go farther, faster, deeper and cheaper”. The overall concept of to demonstrate that sustainable pig TREASURE is devoted to raise innovation potential to give new production systems can be built on perspective and new prospects to pig production by exploiting the local autochthonous breeds, locally treasures encompassed in local pig breeds, their production systems available feeding resources and and products. traditional products corresponding WP1: The information gathered on genetic/genomic constitution of to societal demands for animal local pig breeds, the knowledge of potentially negative alleles, welfare, environment, quality and genetic basis responsible for specific adaptive traits (resilience, health benefits of the products robustness), the development of marker panels (reasonably priced tools) useful to solve traceability problems, the use of gut microbiota as a “footprint” of feeding/management represent innovation potential to serve, set up or improve breeding programmes, in-situ management of genetic resources, and contribute to better utilisation of local pig breeds. WP2: The key innovation potential of WP2 resides in the idée fixe how to assure the sustainability of pig production system in the future (likely to depend upon availability and value of local feeding resources) by means of feeding and management practices (affecting also product quality attributes), where besides the multi-aspect assessment of husbandry systems (indoor, outdoor, organic) some innovative strategies in feeding and management of local pig breeds will be addressed. WP3: Development of toolbox for product quality assessment to valorise products from local pig breeds and comparative assessment of variety of existing or new products with regional identity (including intrinsic cue related to local pig breed) along with the relevant consumer acceptability studies represent innovation potential in service of value chain developments and marketing strategies and prospects. WP4: To be sustainable, pig production system must have economic value. At the end of the chain it is the product that needs to be accepted and re-purchased by the consumer. To identify factors important for the economic viability and to develop marketing strategy is the key innovation potential of WP4. 2.2. IMPACT 2.2.1. Expected impacts 2.2.1.1 Project contribution to expected impacts The objectives and tasks of present proposal are planned to respond to the expected impacts depicted in the call as follows:

Expected impacts WPs Expected result that will contribute to the impacts WP1 WP1 Information on genetic/genomic constitution of local pig WP2 breeds, the need to eliminate negative alleles, breed potential for WP3 Improved in-situ management genetic progress will serve to improve breeding programmes WP4 WP2 Guidelines derived from the results or experience will assist and evaluation of genetic WP5 resources by the farming farmers (associations and advisory service are partners). sector WP3 Quality traits to be introduced into the breeding programmes WP4 Improvements in management based on costs/benefits WP5 Transfer of knowledge and experience from RI activities Productivity and economic WP2 WP2 Feed represents the highest costs for farmers. Information on gains in specialised farming WP4 specific nutritional requirements of local pigs, and on the nutritive systems from the conventional WP5 value of local feed resources (natural and local agro-by-products)

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Expected impacts WPs Expected result that will contribute to the impacts and organic sectors will increase the productivity of this farming systems WP4 Costs/benefits analysis will demonstrate potential for improvements for the chain and society WP5 Transfer of knowledge and experience on how to improve productivity and economic gains WP1 WP1 Genetic characterisation would identify singularity of local pig WP2 breeds allowing a better promotion WP3 WP2 Feeding/management strategies as key factors to enhance Promotion of traditional WP4 sustainability, a must for better exploitation of local pig breeds and/or underutilised breeds WP5 WP3 Quality traits for breeding programmes to promote their use WP4 Economic evaluation/marketing strategies for promotion WP5 Transfer of knowledge and functional networks WP2 WP2 Feeding resources and animal management practices studies WP3 will contribute to enhance benefits for consumers in terms of variety WP4 of pork products, homogeneity of quality level (within WP5 chain/product) and healthiness Increased availability of WP3 Thorough assessment of intrinsic quality and consumer diverse, high quality products, acceptability of traditional pork products (TPP) from various chains e.g. with enhanced health and of innovative TPP with improved healthiness and typicity benefits for consumers attributes are key factors to further increase availability and diversity of products WP4 Consumer/market and distribution channels research is essential to enhance availability of the product on the market WP5 Increasing public awareness and thus demand WP2 WP2 Studied factors are key elements for improving productivity, WP3 product quality and thus economic gains WP4 WP3 Activities for benefits for consumers (quality, healthiness) in Economic benefits for farmers, WP5 traditional and new pork products will lead to economic benefits other types of SMEs and for the breeders, processors and local economy as such regional economies through the expansion or creation of new WP4 Consumer research and identification of marketing strategies products and markets is prerequisite for expansion of high quality products WP5 Transfer of knowledge and creation of functional networks will contribute to enhance benefits for all actors of local chains thereby contributing to regional economy profit

Broader adaption of livestock WP1 WP1 Knowledge of genetic basis responsible for specific to limiting or changing agro- WP2 adaptative traits/resilience/robustness to be adopted in breeding climatic conditions, e.g. By WP5 programmes enhancing robustness through WP2 Promotion of use of locally available feed resources the use of adaptive traits from WP5 Transfer of knowledge about intrinsic genetic potential and landraces and local breeds management strategies to adapt better to agro-climatic conditions WP1 WP1-WP5 Enhanced quality and scope of WP2 European ex-situ and in-situ The overall concept of the proposal is based on product-based WP3 valorisation of local pig genetic resources at European scale and collections/on-farm WP4 management thus will contribute to better on-farm management of their WP5 preservation WP1 WP1 Reasonably priced genetic tools useful for population WP2 management, conservation and evaluation will be provided Enhanced methodologies for WP3 WP2 Trials will address innovative management practices to management, conservation, WP4 improve productivity and product quality WP5 characterisation and WP3 New and joint approach in quality evaluation of high quality evaluation of genetic resources pork products (toolbox including common indicators but specific threshold values for acceptability according to the chain/product considered)

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Expected impacts WPs Expected result that will contribute to the impacts WP4 Cost/benefits analysis to improve management WP5 transfer of knowledge about intrinsic genetic potential and innovative practices WP1 WP1 inclusion of new phenotypic traits into breeding programmes; Increased transfer of genetic WP2 possibility of management local pig breeds based on information material into breeding WP3 from genotyping data programmes, farming WP5 WP2 farming practices pro enhanced use of local pig breeds practices, i.e. identification of useful traits (variation) in WP3 identified traits of pork product quality to be incorporated collections into breeding programmes WP5 transfer of knowledge about intrinsic genetic potential

Increased awareness on the WP4 WP4 Case studies with end-users in different market and regions. value of genetic resources, WP5 Support for policy makers in designing efficient agricultural policy engagement of end-users and with local implication in accordance with the total economic value of contribution to implementation a specific sector (public and social values) of international commitments in WP5 raise public awareness of the value of local pig breeds with the area adequate dissemination approaches WP1 WP1-WP5 WP2 The overall concept of the proposed project is a change in the More extensive use of genetic WP3 resources in agriculture paradigm of pig production suggesting a “switch” to pork chains WP4 which are more extensive with better utilisation of locally available WP5 feed resources and using local pig breeds WP1 WP1 will provide marker panels (reasonably priced tools) useful to WP2 solve traceability problems WP3 WP2 will contribute to better efficiency of local pig breeds WP4 production systems thereby giving grounds for security of provision Overall contribution to food WP5 security by supporting with pork products innovations in breeding and WP3 qualitative, consumer acceptability aspects in relation to farming innovative practices will contribute to better sales and provision WP4 consumer, market research will contribute to product availability and security in provision WP5 increasing public awareness and thus demand

2.2.1.2. Improving innovation capacity To improve the technological level in local pig breeds production systems is one of the key objectives of TREASURE. However, Concept of TREASURE respects technological innovation is only part of innovation process that leads to the key elements of innovation economic growth [these are capability to diffuse and absorb capacity (research supply, technology across public and private sectors, organisational structures absorptive capacity, diffusion and networks in the economy and demand for innovation (OECD, and linkages, demand). 2000)]. The elements of innovation capacity improvement are respected in the concept of TREASURE project. The innovation potential (R&D supply) is displayed under point 2.1.4.2 and the original research addressed in the project meets the demands of the modern society and/or consumer concerns in regard to environment, animal welfare, natural resources, culinary heritage, biodiversity, local self-provision, short distribution chains, on the subject of developments in pig production and/or value of high quality traditional pork products. Diffusion and linkages are addressed by important emphasis on dissemination and knowledge transfer and multi-actor approach through creation of network that involves the partners from academia and non-academia, public and real sectors in majority of European regions interested to develop self-sustaining conservation of local pig breeds by exploiting their inherent attributes. The challenge of creating a common (umbrella) collective trademark of products associated to local pig breeds is a novel approach of this project which is just a first step for further development of competitiveness of regional pork chains based on local pig breeds.

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2.2.1.3. Improving other environmental and socially important impacts The ambition of TREASURE project is to improve sustainability of pig production system based on local pig breeds. “Sustainability of agricultural system” is based on holistic philosophy and denotes resource efficiency and functional integrity and concerns environment, genetic diversity, ethical and social aspects and economic value (Olesen et al., 2000). TREASURE adresses all societal challlenges, from preservation of agrobiodiversity (genetic characterisation of local pig breeds), ethics (welfare), environment and climate changes (LCA analysis) to socio-economic relevance (multi-criteria evaluation of production systems, consumer acceptability studies, marketing strategies and cost/benefit analysis, consequently rural development).

2.2.1.4. Barriers and obstacles to achieve the impacts Running a project to be as effective and efficient as possible always presents challenges and obstacles or barriers. Assuming the project implementation is performed smoothly and effectively (see risk and contingency plan in section 3.2), the barriers ad obstacles that may determine whether and to what extent the expected impacts will be achieved are related to changes of EU or national legislations on  international shipping of biological samples, that could compromise planned biological analyses  changes of EU or national legislation on experiments using animals, that could make not feasible some trials  political issues that might prevent collaboration between EU and associated countries of project partners  changes of regulation on the protection of intellectual rights

In respect of the novelty introduced in H2020, i.e. multi-actor approach, in the present project a big emphasis is given to the care for allocation of resources and clearly defined roles and tasks of partners. It is worth mentioning the special situation of this project, i.e. involvement of local pig breeds associations to facilitate the access to farmers and better dissemination and exploitation of the results. A barrier, related to the nature of the material used in the project (animals) resides in the fact, that we will deal with local pig breeds, in particular untapped ones, where populations are small and dispersed which represents a challenge for organisation of research work.

2.2.2. Measures to maximise impact (WP5) The proposed measures i.e. activities (further detailed in Table 3.1 for WP5) together with multi-actor approach of the project provide a structure that assures an efficient flow of results and knowledge to achieve the expected Measures deployed in impacts (cf §2.1) by diffusion of innovation potential (=project results) across WP5, together with multi- academia and non-academia sectors as well as in the form of transfer of actor network provide a knowledge from advanced to untapped local breed chains. Measures structure which promises the adopted will cover dissemination and exploitation of results (management of efficient flow of results and database(s), publications, intellectual property rights), but also demonstration knowledge across formal and communication activities. Well structured and planned measures are and informal sectors fundamental for the innovation capacity which depends upon adequate including general public. organisational structures/networks to diffuse and absorb technology. 2.2.2.1. Dissemination and exploitation of results Exploitation of TREASURE results will occur across a wide spectrum of end users with the ultimate purpose of promoting sustainable pork chains through better exploitation of local pig breeds and locally available feed resources, enhanced quality and healthiness and market potential of pork products in line with societal demands regarding the environment and welfare of pigs. Dissemination of TREASURE results will occur throughout the life of the project. The overall objective will be not limited to improve technological level but big emphasis will be given to raise public awareness of the value of local pig breeds adopting adequate dissemination approaches. The end-users of TREASURE project outcomes will be:  academia (universities, research institutes)  real sector (SMEs) and other professionals (farmers associations, advisory services)  various stakeholders (NGO, chambers of agriculture, end-users like consumers) and policy makers  local pig breeders (breeding companies and cooperatives) and local breed pork chain stakeholders  general public Our dissemination targets are therefore much wider that merely the local pig breeders and comprise:  transfer of new knowledge within academia sector

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 transfer of knowledge and experience to non-academia sector  transfer of knowledge to informal sectors and policy makers  raise public awareness of the potentials of local pig breeds for sustainable production systems adapted better to societal demands and changing agro-climatic conditions  to increase public awareness of the value of local pig breeds and the derived products Activities to maximise the impact of TREASURE will be organised in a specific project work package (WP5), which has been structured in four tasks in order to reach the objectives stated above: Task 5.1 Publications and organization of meetings Dissemination of all the scientific and technical issues produced by the project shall be assured through scientific publications, organization of specific meetings (symposia, conferences) and/or presentation of TREASURE results at specific meetings and events. Task 5.2 Development of a new collective trademark Development of a new “umbrella” collective trademark of TREASURE project, including all the European breeds and pork chain products involved in the project. The collective trade mark will be designed for the future involvement, after the end of the project, of other organisations, and it will be a start up point for the development of a »sustainable pork chain« quality standard. Task 5.3 Web and media dissemination Web and media dissemination will consist of development of the database on local pig breeds and their products and a web platform. Dissemination will also flow through the website, media and social networks. Task 5.4 Demonstration and transfer of knowledge Transfer of knowledge will flow from academia to non-academia sector (farmers/industry) and also within non-academia sector by means of active networking i.e. organisation of events, training courses with participation of project partners and their associated third parties (but not limited to them). Activities of demonstration i.e. transfer of knowledge and experience will pass from established, successful pork chains to partners in territories where local breed pork chains are at start-up stage.

2.2.2.2. Management of intellectual property rights (IPR) A Consortium Agreement (CA), to which participants will be committed, will enter into force immediately after the project approval and prior to Grant Agreement signing, and will provide the Governance Rules. The Consortium Agreement will govern the interactions among the participants concerning IPR. Furthermore, the IPR management will be carefully defined in the Consortium Agreement, in compliance with the regulation on intellectual property and patenting of each country and each institution involved in the TREASURE consortium. The Scientific Committee will be in charge of the approval of the plans of protection, use and dissemination of knowledge generated by the project. It will be notified about any interest for patenting in order to take any action required to permit and facilitate IPR; e.g. keeping confidentiality, establishing specific agreements among involved participants, management, etc. TREASURE will follow the rules set by the EU in terms of IPR, basically: - pre-existing know-how of the partners, while remaining the sole property of their owners, will be made available to the other partners as needed for the fulfilment of the project - know-how developed by the partners during the project will be owned by the partners who will have contributed to its creation, either alone or jointly with one or more other partners. - all the necessary measures in terms of patenting, collective trade mark protection and publishing will be considered in order to protect the intellectual property. When a patent will be considered, a share of the patent will be recognized to each of the partners which will have participated to the design and development of the patent, both in terms of sustaining the patenting costs, and of benefiting from the potential commercialization of it. A protection of the collective trade mark foreseen within the TREASURE project will be a property of the consortium partners. The subject of the intellectual property rights (IPR) is of great importance. A Consortium Agreement will 18 TREASURE 634476 indicate rules of IPR sharing. TREASURE collective trade mark has been thought as an “umbrella” trademark of the project with aim of including all local pig breeds and their products involved in this project. The collective trade mark is going to be an important exploitation instrument for the project, and its promotion one of the main objectives of the project. It will be extensively advertised both through dissemination events and by means of the web platform. Concerning the dissemination of project results, as a general principle all partners will be committed to The Guidelines on Open access to Scientific confidentiality about findings and will, prior to Publication and Research Data in Horizon 2020 publication, give 45 days advance notice to the other will be respected and policy on open access to beneficiaries and receive written consent from the publications and research data adopted. Scientific Committee. In regard to scientific results, except when legitimate interests will exist, partners will disclose project results to the public as soon as possible. The Guidelines on Open access to Scientific Publication and Research Data in Horizon 20203 will be followed and article 29 of H2020 Annotated Grant agreement (AMGA)4 respected. The Scientific Committee will be responsible for monitoring over proper dissemination and exploitation of results. 2.2.2.3. Communication activities TREASURE partners will promote the project and its results, by means of communication to various audiences (through different media), taking care at the same time of the dissemination, confidentiality and security obligations depicted in AMGA (articles 27, 36, 37, 39). Strategy of communication will reside on the value of the project and its results for the benefits of the sector and society (e.g. competitiveness of the local pig breeds chains, importance for life quality, environment, new products, rural development, job opportunities,...), all in a way that they can be understood by non-specialists. Strategy will also be adapted to the public concerned or to the particularities of the regions. Relevant communication strategy is important to improve the public awareness of the assets of local pig breeds, and market potential of their high quality products. It is essential to develop promotional and communication strategies adapted to specific regional circumstances. Suitable activities will be carried out during the whole project, both internationally and locally. A comprehensive communication plan will be prepared with clear objectives and adapted to target audiences. An activity that is expected to have a ‘major media impact’ will be first notified to the Commission/Agency (monitored by Ethical Committee). An important and strategic role in TREASURE communication will be played by two instruments: the new collective trade mark, and the web platform. Continuous communication will be assured by the integrated web platform to reach various target users (general public, specialists, farmers, policy makers, associations, research organizations, etc.), both at local and at global level; this goal will be achieved also thanks to the development of the platform in different European languages. 2.2.2.4. Data management TREASURE project will create various types of data, observational from surveys or field measurements, experimental data obtained in laboratory conditions, and or metadata derived from the compilation of the literature or acquired from other sources. Data will be stored in various formats, as text, numerical data or otherwise discipline or instrument specific. Each partner will be responsible to adequately store the data acquired from the experiments or field studies, respecting good practice guidelines.5,6 There will be two levels of care for data; first one corresponds to the responsibility of partners to assure relevant organisation, documentation, security and storage of data generated within their tasks, the second is the creation of collective repositories related to TREASURE activities on collaborative platform, administered by management office. The proposal on which data, what type and format of data to be stored in the collective repositories will be prepared by the Scientific Committee, but final decisions will be taken at the General Assembly. Similarly, it will be considered by the Scientific Committee, and final decision taken by the General Assembly, which research data, what type and format of data will be available publicly (as an open-access to research data), as well as how data will be curated and preserved after the end of the project. As consortium has decided to take part in the pilot study on Open Research Data, data management plan will be prepared as a distinct deliverable (D6.3 in the first 6 months of the project).

3 http://ec.europa.eu/research/participants/data/ref/h2020/grants_manual/hi/oa_pilot/h2020-hi-oa-pilot-guide_en.pdf (10.6.2014) 4 http://ec.europa.eu/research/participants/data/ref/h2020/grants_manual/amga/h2020-amga_en.pdf (10.6.2014) 5 http://ec.europa.eu/research/participants/data/ref/h2020/grants_manual/hi/oa_pilot/h2020-hi-oa-data-mgt_en.pdf (21.6.2014) 6 http://www.icpsr.umich.edu/icpsrweb/content/deposit/guide/index.html (21.6.2014)

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2.3. IMPLEMENTATION 2.3.1. Work plan – Work packages, deliverables and milestone 2.3.1.1. Brief presentation of the overall structure of the work plan The activities to reach the objectives (detailed later on) are organised in six WPs with the following management structure: Coordination (WP6) Management office (WP6) Marjeta Čandek-Potokar, KIS Marjeta Čandek-Potokar, KIS Deputy: Luca Fontanesi, UNIBO Deputy: Prof. Loreto Gesualdo, CERTA WP5 Mesures to maximise impact Prof. Loreto Gesualdo, CERTA Deputy: Giuseppe Dalfino, CERTA WP1 WP2 WP3 WP4 Phenotypic and genetic Management and Traditional and new Consumer preferences characterization of perfomance of local pig high quality pork and market research regional autochthonous breeds in their products with regional for sustainable pork pig populations in Europe production systems identity chains Luca Fontanesi, UNIBO M. Čandek-Potokar, KIS Bénédicte Lebret, INRA M. Angels Oliver, IRTA Deputy: Ana Fernandez, Deputy: Rosa M. Nieto, Deputy: Carolina Deputy: José M. Gil, INIA CSIC Pugliese, UNIFI CREDA

Objectives of WP1 Activities to achieve objectives:  Describe the singularity of local pig breeds at Task 1.1 Phenotypic characterisation of regional phenotypic, genomic and functional level autochthonous pig breeds with special attention  Evaluate the adaptation of local pig to the untapped populations populations to agro-climatic conditions Task 1.2 Genetic analyses of the untapped pig  Develop new methodologies for the populations with High Density SNP chips management of the local pig populations (MANAGEMENT TOOL) to determine basic genetic parameters.  Develop DNA based tools for animal and meat Task 1.3 Allele frequencies of known major genes in traceability and authentication regional autochthonous pig populations Task 1.4 Identification of genome regions or genes responsible of adaptive traits and resilience through the comparison of genomes of local, well adapted breeds with the ones of the commercial breeds. Task 1.5 Identification of DNA markers useful for breed authentication and traceability Task 1.6 Pilot functional studies through gene expression analyses Task 1.7 Pilot characterisation of intestinal microbiota Objectives of WP2 Activities to achieve objectives:  Evaluate productivity of different local pig Task 2.1 Collection of data and samples for multicriteria breeds held in production systems with varying evaluation of pig production systems and intensity and geo-climatic conditions comparative analysis of productive traits in local  Assess environmental sustainability of pig breeds (including gut microbiota production systems of local pig breeds and composition) impact of feeding strategies, resources and Task 2.2 The impact of production system on productivity, management considering animal welfare, welfare and environment environmental impact and economic benefits Task 2.3 Nutritional requirements of growing pigs, and  Determine nutritional requirements of local pig lactating sows in selected local pig breeds in breeds to optimize the efficiency and reduce different environmental conditions environmental impact Task 2.4 Innovative feeding strategies or management practices and their effects on production traits  Study innovative feeding and management and product quality practices in view of productivity and product

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quality Task 2.5 Local feeding resources rich in natural  Evaluate locally available feeding resources antioxidants and agro-by-products in the diets and agro-by-products in view of productivity for local pig breeds and product quality  Characterise gut microbiota composition in relation to feeding and management (pilot study) Objectives of WP3 Activities to achieve objectives:  Characterize intrinsic quality, health benefits of Task 3.1 Development of a toolbox for the evaluation of a variety of pork products from diverse sensory, healthy, technological and typical European local pig breed production systems qualities of pork from local pig breeds  To introduce innovations in traditional pork Task 3.2 Evaluation of sensory, healthy, technological products with the aim to develop new or re- and typical attributes of regional pork products discover products of high intrinsic quality and from untapped breeds and new products issued health benefits and expand market share from local chains  Identify links between product (typical) quality Task 3.3 Consumer sensory studies with local pig breed, feeding resources, Task 3.4 Database on carcass and meat quality traits for management, processing techniques, “know- further development of breeding programmes how” and cultural heritage in local breeds  Evaluate consumer acceptability of high quality and innovative traditional pork products from untapped local pig breeds in different market areas  Implement database on carcass and meat quality traits to sustain further development of breeding programmes in local breeds Objectives of WP4 Activities to achieve objectives:  Analyse costs and benefits (private and public) Task 4.1 Cost benefit analysis at farm and product level of production systems along the food chain considering the private component of benefits. based on local and untapped pig breeds. Task 4.2 Cost-benefit analysis for the society considering  Evaluate consumer attitudes and preferences, the public component of benefits motives, willingness to pay for pork products Task 4.3 Consumer Preferences and willingness to pay from local pig breeds for Traditional pork products and New  Evaluate marketing strategies (short chains Traditional pork products and/or direct sale channels) for stimulating the Task 4.4 Marketing strategies and Road Map for an sales of local pork products European Label on Traditional Pork products. Objectives of WP5 Activities to achieve objectives:  Transfer of knowledge and expertise along the Task 5.1 Publications and organisation of meetings chain partners, general public and policy Task 5.2 Development of new collective trade mark makers Task 5.3 Web and media dissemination  Development of new collective trade mark Task 5.4 Demonstration and transfer of knowledge  Creation of platform for functional network within/between regions, sectors  Communication to various publics Objectives of WP6 Activities to achieve objectives:  Coordination of scientific and financial activities Task 6.1 Establishment of collaborative platform according to the work plan Task 6.2 Establishment of management plan, common  Monitoring results to control their scientific protocols and formats for reporting quality with regard to fulfilment of the planned Task 6.3 Scientific management objectives, quality of results, timing, spent Task 6.4 Administrative management resources, and state of the art  Efficiency of organisational setup to support the project  Prepare financial and technical reports for the commission

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2.3.1.2. Timing of different workpackages (Gantt)

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Figure 2: Graphical presentation of inter-dependency between activities

2.3.2. Management structure and procedures The implementation of TREASURE project is highly dependent on an integrated organisational structure, capable of ensuring efficient decision-making and control processes. For this purpose, TREASURE management structure is composed of different and integrated bodies, cooperating in order to provide the appropriate guidelines for in-time project completion and productive resources management. The collaboration between the members of the consortium is driven by:  The large-scale effort required in each work package,  The establishment of a wide network between pork chain actors,  The valorisation of pork chain traditional resources through a new brand,  The profitable dissemination and exploitation plan. 2.3.2.1. Management structure description An appropriate management structure has been designed for the TREASURE Project, in order to guarantee an effective result-oriented decision making structure. As TREASURE Project involves a very large and complex Consortium, the designed management structure allows all Partners to participate actively to the decision making process through the General Assembly, which will support the Coordinator in the management of strategic and crucial issues. The General Assembly guarantees the democracy of the decision making process.

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Scientific issues will be undertaken separately by a specific Scientific Committee. The separation between management and scientific issues guarantees a smoother solution of arising criticisms, involving the most appropriate and skilled people for each kind of issue. Moreover, the day-by-day activities will be carried out by a Management Office, supporting the Coordinator, which will be able to focus on scientific activities and major issues. The following figure displays TREASURE management structure.

Figure 3 TREASURE Management Structure

Management structure The objectives of project management are: • ensuring project compliance with EC rules; • ensuring the effective coordination and collaboration among the scientists within the project; • meeting the objectives of the project within the agreed budget and timeframe; • coordinating project activities and ensuring effective internal communication; • carrying out quality control on the work performed and on deliverables; • providing adequate information to decision making bodies; • informing governing bodies about problems or conflict situations; • setting up an organization to support the above objectives.

A Consortium Agreement will be prepared and will be signed by partners prior to finalising the contract with the Commission. Besides specifying the details of the management structure and of the decision-making process outlined below, it deals with issues related to the contribution of each participant in terms of tasks, human and material and financial contributions, rules for dissemination and use (confidentiality, ownership of results, patent rights, exploitation of results, dissemination of knowledge), financial provisions, and legal provisions.

Operational, decision-making and advisory bodies The following bodies constitute TREASURE management structure: General Assembly, Project Coordinator, Management Office; Work Package Leaders, Scientific Committee, Ethical committee, Quality Control Committee.

Definitions, functions and responsibilities Essentially the structure consists of three major management levels: • The governance level is represented by the General Assembly and the Project Coordinator. The Management Office will support the whole structure. • The operative level is represented by WP Leaders.

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• The transversal level is represented by the Scientific Committee, the Ethical Committee and the Quality Control Committee.

The General Assembly is the decision-making body of the project. Chaired by the project coordinator, it is composed of one representative7 per partner (including WP leaders) each having one vote for decision making. General Assembly takes strategic decisions and is responsible for all decisions concerning structural and organizational changes in order to ensure durable integration, in particular budget revision, modifications to work plan, measures towards default partners. It formally meets annually. Extraordinary meetings can be called by any of its members. Its duties include, in addition to the overall project strategy and management, the fund distribution policy, and major problems solving (e.g. expelling or admitting members). In agreement with the Scientific Committee it will also control that there is no violation of intellectual property of any kind and it will survey ethical and gender issues, with regards to scientific publications, monitor that everybody's contribution is respected and properly acknowledged. General Assembly will also supervise the work of Management Office including quality control.

The Project Coordinator is in charge of all legal and financial matters. It is represented by Dr. Marjeta Čandek-Potokar (KIS) who will adopt all the operational duties of coordination, assisted by the Management Office. Her primary role will be to represent the intermediary between the European Commission (EC) and the Consortium as well as to be promoter and supervisor of the overall progress of TREASURE. The Coordinator regularly monitors the project progress, referring to the General Assembly. All the decisions and reports are developed through its supervision. The Coordinator represents the main contact-point between project partners and EC. In each separate phase of the project the Coordinator will be responsible for: • representing the Consortium in the communication with the EC; • informing governing bodies about problems or conflict situations; • ensuring the cooperation among the partners and guaranteeing that all efforts are focused towards the objectives; • transmitting of reports and other deliverables to the Commission; • timely delivering of cost statements as well as financial audit certificates from the project partners to the Commission; • distributing of any documents/information among the partners, ensuring the constant communication flow; • administering and forwarding to the Commission any requests for the termination of a partner’s participation in the Contract, upon approval of the General Assembly; • transfer of the advance payments and further payments from the EC to the partners as per the provisional budget and the actual expenses approved by the General Assembly; • external affairs and presentation of the project; • organising and chairing various Committee meetings and taking all actions to enable proper decision making.

The Management Office assists the Project Coordinator in his daily activities. At the beginning of the project Management Office will prepare a management plan8 to provide TREASURE partners with practical guidelines for the management of the project. The plan will be prepared under the supervision of the Coordinator and submitted to General Assembly for agreement. Management Office will also prepare the workpackage scorecard to enable Coordinator and General Assembly to easily assess the progress of each workpackage, based on indicators such as punctuality to provide deliverables, to reach milestones and to send reports. It will also allow each WP leader to evaluate the state-of-progress of the respective WP and take corrective measures, when necessary.

Management Office will also prepare collaborative platform, an internet based and secured collaborative workspace where TREASURE partners will share and exchange information. Its purpose is to foster collaboration at all levels and for all bodies of project structure. Its function includes all scientific, administrative and financial information exchange and archiving and will also enable e-voting system.

Main tasks of Management Office concern: • internal communication among partners

7 The person appointed as the main contact of the partner in section 4 8 synthesis of all contractual rules and management procedure

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• project administration i.e. planning, follow-up, preparation of minutes from general assembly and committees’ meetings; • collection and consolidation of administrative and financial documents, periodic reports, • financial administration (monitoring the expenses against budget allocation, consolidation of financial summary sheets etc.) • consolidation and control of the annual cost claims according to contractual requirements, their conformance with the work done and the audit certificate to be pronounced by the partners • assistance to individual project partners on specific administrative issues • management of gender issues

The Management Office will be leaded by the Coordinator and will be operatively supported by CeRTA, a centre of competences in agri-food sector involved as partner of the project.

Coordination of Work Packages (WP) will be assumed by WP leaders who will be in charge of monitoring all activities towards the objectives of the respective WP. They will take operational decisions regarding the WP day-to-day management. They decide, in agreement with the Project Coordinator, about any temporary exchange of personnel and their duration. They are in charge of preparing the decisions to be taken by the General Assembly and ensuring that these decisions are properly implemented. They are also in charge of addressing and documenting internal risks which may impair the progress towards the objectives of the WP and suggest strategies to minimise such risks. WP1 will be lead by Luca Fontanesi (UNIBO), a deputy will be Ana I. Fernández (INIA) WP2 will be lead by Marjeta Čandek-Potokar (KIS), a deputy will be Rosa M. Nieto (CSIC) WP3 will be lead by Bénédicte Lebret (INRA), a deputy will be Carolina Pugliese (UNIFI) WP4 will be lead by Maria Angels Oliver (IRTA), a deputy will be José M. Gil (CREDA) WP5 will be lead by Loreto Gesualdo (CERTA), a deputy will be Giuseppe Dalfino (CERTA)

The responsibilities of the Work Package Leaders are: • monitoring the progress of the WP against time and budget allocations, ensuring that it fulfils the objectives listed as milestones and deliverables, alerting in case of delay or default; • gathering the material from all partners participating in their respective Work Package in order to produce the deliverables and reports according to work plan • delivery of annual management reports to the Project Coordinator; • preparation of proposals for the update of the work plan; • preparation of proposals for new Parties (if needed);

Scientific Committee is composed of the scientific leaders of the work packages. Its role is to supervise scientific aspects and exploitation of the results of TREASURE project and to propose to the Coordinator and to the General Assembly new orientations. Jointly with the General Assembly it will guarantee that IPR are properly addressed and respected. Beyond scientific monitoring, a key role of the Scientific Committee is to constantly supervise the activities related to the exploitation of results through dissemination, transfer of knowledge and the valorisation of project results, including development of new brand.

Ethical Committee is composed of 3 members. Its role is to ensure proper carrying out of animal experiments and handling of biological samples and genetic data. The Ethical Committee will supervise the attainment of favourable opinions by the relevant local Ethics Committees and will report about the attainment of such favourable opinions providing a report containing a copy of the official approvals to the Commission. Ethical committee will also monitor the communication strategy of the project and its results, and address emergent issues. Members of the Ethical Committee will be nominated after the Consortium Agreement signing.

Quality Control Committee is composed of 5 members nominated from the partners (after the Consortium Agreement signing). It will be responsible for the internal control of the progress of TREASURE work plan, to identify tasks or partners at risk (low, medium, high) and to suggest corrective actions. In the case of the conflict of interests, respective member will be replaced by the neutral member.

2.3.3. Consortium as a whole Considering the multidisciplinary, very comprehensive and multi-actor approach characterising the project, TREASURE will involve 25 partners from various sectors (academic, SMEs, advisory service, chamber of

26 TREASURE 634476 agriculture, farmers associations) and from 8 member States (Slovenia, France, Germany, Croatia, Italy, Lithuania, Portugal, Spain) and one associated country (Serbia). The aim is to create a network of excellence/competence thanks to the transfer of knowledge from the academia to non-academia, and from established pork chains and untapped breed pork chains at start-up stage. Each partner has its own expertise and can participate to the project with different roles and functions, giving a specific contribution in different project areas, clustered as follows:

To achieve scientific goals TREASURE academia partners have all the relevant competences (on animal sciences, genetics, nutrition, pig production systems, environmental impact, meat quality/technology, consumer science, agro-economics and policy) necessary to achieve the expected results, and possess the necessary complementarities. Details on their expertise are given in annexe, section 4 and are visible also in the respective responsibilities within work plan:  Public Research Institutions - Agricultural Institute of Slovenia, Slovenia - Institut National de la Recherche Agronomique, France - Institute for Animal Husbandry Belgrade-Zemun , Serbia - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Spain - Agencia Estatal Consejo Superior de Investigaciones Cientificas, Spain - Centre de Recerca en Economia i Desenvolupament Agroalimentari, Spain - Centro de Investigaciones Cientificas y Tecnologicas de Extremadura, Spain - Institut de Recerca i Tecnologia Agroalimentàries, Spain - Instituto Politecnico de Viana do Castelo, Portugal  Universities - University of Ljubljana, Biotechnical faculty, Department of Animal Science, Slovenia - University of Zagreb, Faculty of agriculture, Croatia - University of Osijek, Faculty of agriculture, Croatia - Universita di Firenze, Dipartimento di Scienze delle Produzioni Agroalimentari e dell’Ambiente, Italy - ALMA MATER STUDIORUM – University of Bologna, Department of Agriculture and Food Sciences, Italy - Lithuanian University of Health Sciences, Institute of Animal Science, Lithuania - University of Evora, Portugal - University of Belgrade, Faculty of Agriculture, Serbia

When building up the consortium a special emphasis was given to real and informal sector which operates in local pig breeds chains, as well as to centres of competence, advisory institutes, chamber of agriculture as actors assuring the best exploitation and transfer of results and knowledge (also via “collective trade mark”).  Producers - Bäuerliche Erzeugergemeinschaft Schwäbisch Hall, Germany - Eco Village, Dudovica, Serbia  Associations - Associazione Nazionale Allevatori Suini, Italy - Asociacion Española de Criadores de Cerdo Iberico, Spain  Centers of competence or advisory services - Ce.R.T.A. Centri Regionali per le Tecnologie Agroalimentari, Italy - SSICA, Stazione Sperimentale per l’Industria delle Conserve Alimentari, Italy - Chamber of Agriculture and forestry of Slovenia, Slovenia - IFIP – Institut de porc, France - AGRIS Sardegna, Agenzia Regionale per la Ricerca in Agricoltura, Italy

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In addition to partners, also linked third parties are involved in the network:  Academic Research Centres - University of Sassari, Veterinary Medicine Department (UNISS), Italy - Institute of Meat Hygiene and Technology, Serbia - University of Extremadura, Spain Moreover, in TREASURE, the involvement of informal sector, i.e. local pig breeds associations (as third parties), is foreseen as a support and further proof of the interest in the sector to develop these chains and of the need for a close collaboration between the sectors.  Associations and consortia - Association of breeders of Krškopolje pigs, Slovenia - Plemenita opcina turopoljska (POT), Croatia - Agricultural service cooperative – LETA, Croatia - Lithuanian Endangered Farm Animal Breeders Association, Lithuania - Associação Nacional de Criadores de Suínos da Raça Bísara (ANCSUB), Portugal - ANCPA (National Alentejano Pig Breeders Association), Portugal - Consorzio di Tutela della Cinta Senese, Italy

It is also worth noting the important involvement of countries, where local pig breeds can be considered untapped and where the construction of the whole chain approach in management of the breed is only at start-up stage. The role model of partners from “developed” chains9 is supposed to help in building up the capacities of the partners dealing with untapped local pig breeds. Thus in the TREASURE project a collaboration with associations, farmers, chambers of agriculture, and SMEs (field surveys and samplings, consumer studies, support in dissemination and promotional activities) is foreseen to carry out the activities. By integrating existing network of scientists and their national links, new interactions will emerge, between real and informal sectors of different regions, which is one of the important aims of the project.

TREASURE addresses the aspects of high relevance for the development of local agro-food sector as demonstrated by past and on-going national projects, EU research and rural development projects (cf 1.3.3) in which partners of the present proposal were included; at the same time it demonstrates relevant competences of the consortium to fulfil the foreseen tasks.

The Consortium of TREASURE is composed by sub-teams which will carry-out each work package and finally the whole project. The project management work package is occupied by WP leaders, while all partners’ contributions to the smooth project’s progress are required.

The Consortium is comprised of a wide and different range of expertises, with partners fitting each WP with required skills; their contribution and complementarities are summarised in table below.

9 TREASURE network was initiated from the Mediterranean pig network

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WP 1 WP 2 WP 3 WP 4 WP 5 WP 6 1.1 1.2 1.3 1.4 1.5 1.6 1.7 2.1 2.2 2.3 2.4 2.5 3.1 3.2 3.3 3.4 4.1 4.2 4.3 4.4 5.1 5.2 5.3 5.4 6.1 6.2 6.3 6.4 1-KIS C L C C L C C C C C C C C C L L L L 2-UNILJ C C C C C C C C 3-KGZS C C C C C C C C C 4-IFIP C C C C C L C C C C 5-INRA C C C L C L C L C C C C C C C C C C C C C 6-BESH C C C C C C C C C C C C 7-UNIZG C C C C C C C C C C C C 8-UNIOS C C C C C C C C C C 9-AGRIS C C C C C C 10-ANAS C C C C C C 11-UNIFI L C C C L C L C C C C C C C C C C C C 12-CERTA L L L L C C C C 13-UNIBO C C L L C C C C C C C C C C 14-SSICA C C C C 15-LUHS C C C C C C C C C C C C 16-IPVC C C C C C C C C C C C 17-UNIEV C C C C C C C C C 18-IAH C C C C C C C C C C C C C 19-UNIBG C C C C C C C C C C C 20-INIA C L L C C L C C C C C C C C C C C C 21-CSIC C C L C C C C C C C C C 22-CREDA C L L C L C C C C C C C 23-CICYTEX C C C C C C C C C 24-IRTA C C C C C L C C L C C C C C C C C 25-AECERIBER C C C C C Position of partners in the tasks of each WP (L= Leading, C= contributing)

2.3.4. Capacity of participants and links to third parties 2.3.4.1 Participants

Partner 1 - KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS)

Description of the organisation: KIS is a public research institution founded in 1898. It carries out basic and applied research, and provides laboratory service, knowledge transfer and expert work for end-users (advisory centres, government, policy makers, chamber of agriculture, farmers, industry) in the field of agriculture and food quality. KIS is organised in nine departments (Animal Science, Crop Science, Plant Protection, Fruit, vine growing and oenology, Agricultural economics, Agricultural engineering, Agroecology and Central laboratories) and employs 175 persons, of whom 84 are researchers. Research activities are carried out within two research programmes, Agrobiodiversity and Sustainable agriculture. KIS has been a coordinator of many national projects, and has participated in many projects of different EU programmes, from FP5, FP6, FP7, Life, Interreg, European Territorial Co-operation etc., and has also experience in coordination of EU projects (AGROTUR, CROPSUSTAIN). Competences: KIS has long track of experience in pig production; its expertise is particularly important on factors in pig production and how they affect product quality. The competences can be portrayed by the topics of past and current projects on pig carcass classification, novel technologies for body composition and meat quality evaluation i.e. NIRS, pig genotype for dry ham production, market research for traditional dry ham; presently KIS is involved in research related to ban on castration i.e. on immunocastration and measures for boar taint reduction and emerging problems of product quality. KIS also initiated studies of productive traits and quality of products in Krškopolje breed. Main tasks in the proposal: KIS will coordinate the project, WP6 and WP2. Participation in WP1 will comprise activities related to phenotypic characterisation of Krškopolje breed and sample collection. Research

29 TREASURE 634476 activities in WP2 will cover data collecting, overall statistical analysis and compilation, experiments with local feeding resources in cooperation with other Slovenian partners. In WP3, KIS will contribute to the development of toolbox and assessment of the quality of products from Krškopolje breed including novel technologies (NIRS). With local products it will participate in the WP4 dedicated to consumer and market research. KIS will be involved also in WP5 activities of dissemination and active project networking. KIS will be involved in ASD conference organization, presentation TREASURE at yearly agro-food fair AGRA, demonstration activity for association of breeders.

Profile of the most relevant staff members who will be undertaking the work

Marjeta Čandek-Potokar (1965, Female) Current position at KIS: scientific counsellor; coordinator of research programme “Sustainable agriculture” Main research activity in animal production (Pig, Beef), muscle biochemistry, meat quality. 2004: Assistant professor at University of Maribor, Slovenia 1997: PhD in Food science, University of Ljubljana, Slovenia 1991: MSc in Food science, Universite Blaise Pascal, Clermont-Ferrand, France 1988: Engineer of Zootechnics, University of Ljubljana, Slovenia Coordinator of 6 national projects, partner in EU projects FP5–EUPIGCLASS, FP6–TRUEFOOD, FP6-YOUNG- TRAIN, FP6–PIGCAS, COST FA1102 FAIM. Participates in tasks of pig breeding programme (carcass and meat quality evaluation), the member of scientific council of approved breeding organisation for pigs; Slovenian representative in Expert group for pig carcass classification at EC. From 2004, habilitated at Maribor University, Faculty of agriculture and life sciences, as lecturer of Technology of animal products and mentoring BSc, MSc, PhD works. Author of >50 peer-reviewed articles; h-index: 11.

Martin Škrlep (1979, Male) Current position at KIS: scientific collaborator. Research subjects related to different aspects of pig and cattle breeding including carcass quality, quality of meat and meat products, breeding conditions, immunocastration, proteomics and sensory evaluation. 2012 Assistant professor at University of Maribor, Slovenia 2010 PhD in Food Science, University of Ljubljana, Slovenia 2004 Dr. vet. med., Veterinary Faculty, University of Ljubljana, Slovenia Involved in national and EU projects (TRUEFOOD,COST FAIM) and in tasks of carcass and meat quality evaluation for pig breeding programme and carcass classification. Lecturer of Anatomy and physiology of domestic animals at Faculty of agriculture and life sciences (University of Maribor). Author of >20 peer- reviewed articles:; h-index: 7.

Nina Batorek-Lukač (1985, Female) Current position at KIS: young researcher. Research subjects related to different aspects of pig breeding including carcass quality, quality of meat and meat products, immunocastration, pig metabolism and nutrition. Graduated from Veterinary Faculty, University of Ljubljana, Slovenia in 2009 is presently a doctorant on co- tutelle Ph.D (Faculty of Agriculture and Life Sciences at University of Maribor and Agrocampus Ouest, Rennes, France) thematics: Immunocastration as a way to prevent meat boar taint: influence on growth performance, body composition and meat quality. Number of publications in international peer-reviewed journals: 5; h- index: 2

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. ČANDEK-POTOKAR M, ŠKRLEP M. Factors in pig production that impact the quality of dry-cured ham : a review. Animal, 2012, 6, 2, 327-338. 2. ČANDEK-POTOKAR M., ŽLENDER B., KRAMAR Z., ŠEGULA B., FAZARINC G., URŠIČ M. Evaluation of Slovene local pig breed Krškopolje for carcass and meat quality.Czech Journal of Animal Science, 2003,. 48,. 3, 120-128. 3. KASTELIC A., ČANDEK-POTOKAR M. Application of quality labels in support of conservation of local breeds - a challenge for Slovenian Krškopolje pig. In: ČANDEK-POTOKAR M. (Ed.).8th International Symposium on the Mediterranean Pig, Slovenia, Ljubljana, October 10-12, 2013, (Acta agriculturae slovenica, Supplement, 2013, 4). Ljubljana: Biotechnical Faculty, 2013, suppl. 4, 205-209.

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4. PREVOLNIKM., ŠKRLEP M., JANEŠ L., VELIKONJA BOLTA Š., ŠKORJANC D., ČANDEK-POTOKAR M. Accuracy of near infrared spectroscopy for prediction of chemical composition, salt content and free aminoacids in dry-cured ham.Meat science, 2011, 88, 2, 299-304. 5. BATOREK N., ŠKRLEP M., PRUNIER A., LOUVEAU I., NOBLET J., BONNEAU M., ČANDEK-POTOKAR M. Effect of feed restriction on hormones, performance, carcass traits, and meat quality in immunocastrated pigs. Journal of animal science, 2012, 90, 4593-4603.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

Project name Programme Duration AGROTUR Kras destination of autochtoneus products Slovenia-Italy; European 2010-2014 Territorial Co-operation L4-5521 Raising entire males or immunocastration: research on National project 2013-2016 measures for boar taint reduction and emerging problems of product quality TRUEFOOD Traditional United Europe Food; FP6 2006-2010 WP4 Use of genetic markers to breed pigs adapted for production of low salted dry hams V4-0864 Market prospects for traditional Prekmurje dry ham National project 2003-2005 EUPIGCLASS Standardisation of pig carcass classification in the FP5 2000-2003 EU through improved statistical procedures and new technological developments

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work KIS disposes with laboratories equipped to perform various meat/product quality analysis (Kjeldahl, Soxhlet system, atomic absorption spectrometer, GC-MS, HPLC-tandem MS, HPLCs with UV/VIS, fluorescent, detectors, NIR spectrometers 6500, Labspec 5000, Lloyd Texture analyser etc.) and has access (co-owner) to the proteomic platform (Ettan IGPhor 3IEF, SDS Page Ettan Dalt Six, Image Master).

Partner 2 - UNIVERZA V LJUBLJANI (UL)

Description of the organisation

The University of Ljubljana (UL) was established in 1919 and is the biggest public university in Slovenia with more than 50.000 undergraduate and graduate students. Due to its dedication to high quality research and teaching, University of Ljubljana belongs to the top ranked universities in Eastern Europe. Researchers at UL were partners in more than 400 EU projects, participate in 8 centres of excellence, and 7 centres of competence funded by the EU structural funds. The Biotechnical Faculty is a constitutive member of the University of Ljubljana and provides university level, advanced professional, and postgraduate education in the wide area of life sciences. The research at Biotechnical Faculty is oriented towards basic topics in biology and related sciences with the aim to bridge basic research with applied research in the field of agriculture and food processing. Among the Faculty departments the Department of Animal Science is performing basic and applied research in the field of animal production, dairy science and agricultural economics and animal biotechnology. The department is also offering undergraduate, MSc and PhD programmes in animal science. The research activities include animal genetics and genomics, animal biotechnology, immunology, animal nutrition, dairy research, biostatistics, environmental issues and agricultural economics. The aim of departmental research activities is aquisition of new knowledge for the advancement of aniaml production and sustainable use of animal genetic resources. The researchers are organized into three research programmes and are involved in a number of international research projects, and represent the department in different international scientific and expert bodies.

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Main tasks in the proposal The research team of UL, Department of Animal Science will participate in the TREASURE project within the WP 1 which is dedicated to phenotypic and genetic characterization of genetic resources involved in the project. Our main tasks will be focused on analysis of allele frequency of known major genes (IGF2, RYR1, MC4R, PRKAG3, LEPR, MC1R, KIT, CALP) affecting phenotypic traits in pigs through the analysis of causative polymorphisms; identification of genome regions and genes responsible for adaptive traits through the comparison of genome sequencing data of local - well adapted breeds with that of commercial breeds based on whole genome sequences of several animals per breed. We will attempt to identify markers useful for breed authentication and traceability (this task will link our activities to WP3) using porcine 60K SNP BeadChip data and genome sequencing data. This will allow the construction of a cheap genotyping tool containing small sets of informative SNPs. Our group will also participate in a pilot functional study with the aim to reveal specific gene expression profiles which could be associated with population-specific biological processes responsible for productive traits and product quality. The analysis will be based on RNA-Seq technology and the most promising candidate loci will be analyzed by quantitative PCR. In WP5, our group will be involved in task 5.1 (publications and participation on meetings). UNILIJ will be involved in ASD conference organization, presentation TREASURE at yearly agro-food fair AGRA, demonstration activity for association of breeders.

Profile of the most relevant staff members who will be undertaking the work

The research group at Department of Animal Science is focused on different aspects of applied animal biology with international expertise in genetics/genomics, immunology, bioinformatics and biostatistics. Peter Dovč is full professor in Genetics, Animal biotechnology and General Animal Science. He obtained his PhD from Technical University Munich in 1988, spent his post doc training at Georg August University in Giessen, Ludwig Maximilian University in Munich and was guest scientist at University of California, Davis in 1994-1995. Since 1995 he is leading the research group in Animal Genetics and Comparative genomics at Department of Animal Science, Biotechnical Faculty, University of Ljubljana. Between 2005 and 2010 he was president of the Scientific council of the National Research Agency. In 2007 he received the national research award. His main research interests are biology of lactation, genetics of complex traits related to animal production and conservation genetics. He published over 100 scientific papers and book chapters in international scientific publications. Mojca Narat is full professor in Immunology. She obtained her PhD in Microbiology and immunology from University of Ljubljana in 1994. She is also guest professor at the University of Novi Sad and head of the Cell laboratory at Department of Animal Science. Her main research interest is immune response to mycoplasma infections in birds and mammals as well as the study of causes for autoimmune diseases. Simona Sušnik Bajec is assistant professor in Animal Biotechnology. She obtained her PhD in Biochemistry and Molecular Biology from University of Ljubljana in 2001. Her main research interest is conservational genetics of Salmonid species and genomics of complex traits in aquatic organisms. Tanja Kunej is associated professor in Genetics. She got her PhD in Biochemistry and Molecular Biology from the University of Ljubljana in 2002. She set up a group working in Bioinformatics at Department of Animal Science. Her research interest is focused on integration of different types of information using bioinformatics tools. Gregor Gorjanc is assistant professor in Animal Breeding. He obtained his PhD from the University of Ljubljana in 2010 and is leading a group estimating breeding values using genomic selection. His main research interest is integration of genomic data into breeding strategies in different animal species. Tatjana Kavar is researcher in the group working on genotyping, DNA sequencing and data analysis. She got her PhD form University of Ljubljana in 2001. She is working in the field of molecular markers and population structure analysis for more than 10 years. Her research interest is focused on estimation of population parameters in different species and development of marker systems for paternity testing and pedigree verification. Jernej Ogorevc is a post-doctoral fellow in the lab and is working on special databases collecting data relevant for the study of complex traits in farm animal species.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal 1. JEVŠINEK SKOK, Daša, GODNIČ, Irena, ZORC, Minja, HORVAT, Simon, DOVČ, Peter, KOVAČ, Milena, KUNEJ, Tanja. Genome-wide in silico screening for microRNA genetic variability in livestock species. Animal genetics, ISSN 0268-9146, 2013, 44 (6): 669-677. 2. OGOREVC, Jernej, DOVČ, Peter, KUNEJ, Tanja. Comparative genomics approach to identify candidate genetic loci for male fertility. Reproduction in domestic animals, ISSN 0936-6768, 2011, 46: 229-239.

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3. KUNEJ, Tanja, WU, Xiao-Lin, MICHAL, Jennifer J., MILOŠEVIČ BERLIČ, Tamara, JIANG, Zhihua, DOVČ, Peter. The porcine mitochondrial transcription factor a gene : molecular characterization, radiation hybrid mapping and genetic diversity among 12 pig breeds. American journal of animal and veterinary sciences, ISSN 1557-4555, 2009, 4 (4): 129-135. 4. The Bovine Genome Sequencing and Analysis Consortium, ELSIK, Christine G., DOVČ, Peter, RAZPET, Andrej, et al. The genome sequence of taurine cattle : a window to ruminant biology and evolution. Science, ISSN 0036-8075, 2009, 324 (5926): 522-528. 5. KUNEJ, Tanja, WU, X-L., MILOŠEVIČ BERLIČ, Tamara, MICHAL, J.J., JIANG, Z., DOVČ, Peter. Frequency distribution of a Cys430Ser polymorphism in the Peroxisome Proliferator-Activated Receptor-Gamma Coactivator-1 (PPARGC1) in Chinese and Western pig breeds. Journal of animal breeding and genetics, ISSN 0931-2668, 2005, 122: 7-11.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

INTEGRATOMICS Time Platform: http://www.integratomics-time.com/home Web based platform for integration of different types of data, originating from different –omics studies (genomics, proteomics, metabolomics, transcriptomics, phenomics) with phenotypic data related with production or health traits. The INTEGRATOMICS Time platform builds on comparative approach and integrates also epigenetic data as methylation profiles, miRNA regulation and chromatin structure data. The package includes also visualization tools which enable interested researchers rapid and user friendly analysis of large data sets.

DNA genotyping and sequencing unit: The unit is equipped with capillary DNA Analyzer (ABI 3100lx) and real time PCR (ABI Via-7) for fast genotyping and classical DNA sequence analysis.

Partner 3 - Kmetijsko gozdarska zbornica Slovenije, Kmetijsko gozdarski zavod Novo mesto (KGZS-NM)

Description of the organisation

Agricultural and Forestry Institute Novo mesto (KGZS-NM) is a subsidiary institute of the Chamber of Agriculture and Forestry of Slovenia, the umbrella interest organization of natural and legal persons in the Republic of Slovenia engaged in agriculture, forestry and fishery. Main activities of KGZS-NM subsidiary unit in Novo mesto involve advisory service for farmers on all aspects of agriculture. As regards animal production, KGZS-NM subsidiary unit in Novo mesto is involved in advisory service and selection work on pigs and cattle in the Southeast Slovenia. The Institute has a staff of 70 people, most of them with higher degree education. KGZS-NM subsidiary unit in Novo mesto is experienced in the area of agricultural and traditional food marketing and is the only institute in Slovenia carrying out work on the development of local Krškopolje pig breed.

Main tasks in the proposal

The KGZS-NM with its experience will be involved in characterization of local Krškopolje pig breed providing various data (anatomical, metabolic, production traits, etc.) and samples (DNA, feces etc.). It will participate (together with KIS) in experiments on pig management and feeding practices (WP2) and in development of high quality meat products from Krškopolje breed (WP3). KGZS-NM with its experience in the area of agricultural and traditional food marketing will be involved in consumer acceptability studies. It will also be involved in project dissemination and demonstration activities (e.g. publishing, presentation of project results on national level, workshops with local pig breeders etc.). It will organize demonstration activities and study trip(s) for breeders of Krškopolje pig to partners in TREASURE project network. KGZS-NM will be involved in presentation TREASURE at yearly agro-food fair AGRA, demonstration activity for association of breeders etc..

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Profile of the most relevant staff members who will be undertaking the work

Andrej Kastelic (MSc.) has been working for 13 years as a specialist for pig breeding at KGZS-NM (Unit in Novo Mesto). His BSc and MSc work was accomplished on Krškopolje breed. Presently he is in charge of the Department for agricultural advice and animal production at KGZS- NM. Zdenka Kramar (MSc.) worked for many years as pig breeding advisor, lately she has been involved mainly in regional development and was responsible for several projects (ERDF-INTERREG III A project (1536-06- 400007), project 4. EKSRP and LEADER-LAS; LIFE: NATURA 2000 in Slovenia–Management Models and Information system«, partner in EU, IEE: BIOMASSTRADECENTREII). Stane Bevc graduated as zootechnical engineer in 1980 and has many years of experience in different fields of animal production, husbandry, breeding and nutrition (pigs and cattle).

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Kastelic A., Čandek – Potokar M. 2013. Aplication of quality labels in supportof conservation of local breeds – a challenge for Slovenian Krškopolje pig. Acta Agriculturae Slovenica, Supplement 4, 2013, 8th International Symposium on the Mediterranean Pig: 205-209 2. Granda S., Simončič J., Kastelic A. 2011. Naše kmetijstvo. Zbornik ob 10-letnici KGZS-Zavoda Novo mesto. Novo mesto, KGZS-Zavod NM: 295 3. Kastelic A. 2001. Telesna sestava prašičev krškopoljske pasme. Diplomska naloga. Ljubljana, Biotehniška fakulteta, Oddelek za zootehniko: 55 4. Kastelic A. 2008. Razvoj pasme in plodnost krškopoljskega prašiča. Magistrsko delo. Ljubljana, Biotehniška fakulteta, Oddelek za zootehniko: 206 5. Štuhec I., Kastelic A., Sever S., Vogrin Bračič M, Pribožič P. 2005. Ureditev hlevov za rejo prašičev na slovenskih kmetijah. The pig housing systems on Slovenian family farms. V: Spremljanje proizvodnosti prašičev. Domžale, Biotehniška fakulteta, Oddelek za zootehniko: 55-73

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal - 2002-2013: The systematic work on the Krškopolje breed, which was facing a disappearance, resulted in big achievements as demonstrated by the increase of number of farms breeding Krškopolje pig (from 30 sows held on 3 farms to the present situation with 300 sow on 70 farms) - 2013: development of quality label for the products from Krškopolje pig

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Disposes with a conference room for 60 people, has a direct access and a network to all pig breeders of Krškopolje breed. For the purposes of the project equipment for monitoring (e.g. ultrasound device for measuring thickness of back fat) is available.

Partner 4 - IFIP - INSTITUT DU PORC (IFIP)

Description of the organisation

IFIP French Institute for pig and pork Industry (http://en.ifip.asso.fr/) is an R&D company (private association) for all professional and economic organisations of pig and pork sector (pig producers, feed industry, genetic companies, slaughterhouses, butcher craftsmen and processing firms). With a team of 80 scientists, IFIP leads applied research and studies to produce knowledge. IFIP has 4 main fields of activities: economics, pig farming techniques, fresh meat and processed products and genetics. Since the 1980s, IFIP (formally ITP) has played a key role in the implementation of preservation programs of local pig breeds. The pig breeding department still provides a technical and scientific support to the LIGERAL,

34 TREASURE 634476 the herd book of the 6 French local pig breeds: development and management of the database, edition of mating plans, assessment of the genetic variability, farms visits. IFIP closely collaborates with the French local chain actors (breeders associations of the LIGERAL). IFIP is also strongly involved in breeding programs and genomics research programs of the major French breeding companies. The fresh meat and processed products department works on all aspects of quality studying pig breeding up to fresh meat preparation and meat processing in close relations with meat sector enterprises: IFIP has expertise in carcass grading, safety of pork products such as technological, sensory and nutritional quality traits. Part of the activity deals with the identification of new predictors of meat quality (NIR spectroscopy). The Computing department develops software and Web database applications in connection with the different IFIP departments and with the field actors in various areas: economics (GTE), monitoring of pig herds (GTTT), feed formulation (Porfal), methanation (Methasim), genetics (molecular data base, herd books) etc.

Main tasks in the proposal IFIP will make the link between the partners of the project and the French local chain actors, especially the Gascon breeders of the Consortium du Noir de Bigorre. In particular, IFIP will take part in the phenotypic characterization of the Gascon breed (tasks 1.1 and 2.1), in sample collection (DNA in WP 1), in setting up experiment on the Gascon breed (tasks 2.5), in the development and use of the toolbox for the evaluation of meet quality traits (tasks 3.1, 3.2). IFIP will be in charge of the development of the database on carcass and meat quality traits (task 3.4). Finally, IFIP will contribute to the dissemination of the results of the project (WP5) with a session at JRP (Journees de la Recherche Porcine) in France, with a workshops during meetings of the Ligeral (herd book) members, with a presentation at Paris International Agricultural Show, with trade fair for food security in Toulouse “Sisqa” and “Tables du Rheu” in Rennes.

Profile of the most relevant staff members who will be undertaking the work

Marie-José Mercat works at IFIP in genetics since 2001. Her main fields of activity are genomics programs and the preservation of local breeds. Regarding to the local breeds, she provides a scientific support to Herveline Lenoir and the LIGERAL, especially on genetic variability analyses, gene banks, genomics and molecular data. She has been involved in the FP7 Q-Porkchains and is responsible for the pig tasks in CRB- Anim (see projects). Concerning genomics, she coordinates projects at interface between major French breeding companies and INRA (e.g. UTOPIGE, see projects). She is responsible for SG Boar taint (see projects) using the expertise she acquired in reproduction as head of R&D for Cobiporc, an artificial insemination centre (1994-2001). She is member of the COST network PiGutNet on microbiota.

Herveline Lenoir is full time in charge of local breed topic at IFIP since 2001. She advises the breeders associations of the LIGERAL, visits farms, manages the database, produces mating plans. She is involved in VARAPE and CRB-Anim (see projects) and was involved in the FP7 Q-Porkchains. Antoine Vautier studied agronomic engineering and animal science in the University of Rennes. He works at IFIP in Le Rheu (France) since 2002 on meat quality. His main research fields are predicting methods for meat quality and porcine myology. Since 2007, he is involved in several research programs based on NIRS spectroscopy. He is involved in the technical support in fresh meat sector (Bigard, Cooperl, Socopa) and the meat processing sector (Madrange, Aoste, Fleury Michon, Herta). He has led projects on innovative tools for pork meat quality prediction (CASDAR project QUALIPORC, see below). Elias Zahlan is responsible for the IFIP computing department. Software and Web database applications are developed with database management systems and tools like Oracle, Nhibernate, OLAP, SQLSERVER, NET, Visual Studio, C++ or C#.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Vautier A., Gault E., Lhommeau T, Bozec A. (2014). Meat quality mapping of the loin: pH vs NIR spectroscopy to predict the cooking yield. 60th International Congress of Meat Science and Technology, Uruguay. 2. Vautier A., Lhommeau T, Daumas G. (2013). A faisability study for the prediction of the technological quality of ham with NIR spectroscopy. 64th EAAP, Nantes, France. 3. Mercat M.J., Hassenfratz C., Muller N. (2013). Genetic effects on boar taint ; EAAP Working Group, Production and utilization of meat from entire male pigs, Monells, Spain.

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4. Lenoir H., Mercat M.J.. Assessment of numbers, reproductive performances and genetic variability of the 6 local breeds. TechniPorc, Vol. 31, 2008, pp. 15-22. These studies are regularly updated even not published. 5. Zahlan E., Lenoir H. Development and management of the Web database of the herd books of the French pig local breeds.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

 VARAPE (2012-2014) funded by CASDAR: Development of low size breeds thanks to short circuit. This project involves several species and several French technical institutes (for pigs, H. Lenoir).  CRB-Anim (2013-2020) funded by ANR: use of semen from the national cryobank as a management tool of then genetic diversity in local breeds (for pigs M.J. Mercat., H. Lenoir).  UTOPIGE (2011-2014; genomic selection) and related projects QUALIPORC (2013-2015; high throughput meat quality tools) and SG BOAR TAINT (2012-2014; boar taint impact on reproduction) funded by ANR, BIOPORC, CASDAR, France Agrimer, Inaporc (M.J. Mercat., A. Vautier). IFIP closely collaborates with INRA (J. Riquet, B. Lebret involved in Treasure) on these projects.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Use of Microsoft technologies: SQLSERVER database, Dotnet framework, C# programming language, Visual Studio environment.

Partner 5 - INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

Description of the organisation

The French National Institute for Agricultural Research (INRA) is a public research institute, ranked number one agricultural institute in Europe. INRA carries out mission-oriented research for high-quality and healthy food, competitive and sustainable agriculture and preserved environment. INRA produces scientific knowledge that leads to economic and social innovation, and lends its expertise to public decision-making from the local to the international level. INRA-PEGASE (Physiology, Environment and Genetics for the Animal and Livestock Systems) ultimate goal is to improve farming practices to meet societal requirements regarding pig production, including product quality and sustainability of production systems. INRA-GenPhySE researches (Genetics, Physiology and Livestock Systems Unit) are dedicated to understand the genetic determinism of traits observed in farm animals and to develop methods and tools for the genetic improvement and conservation of farm animals. INRA-GABI (Animal Genetics and Integrative Biology) has launched programs to study the interplay of porcine traits with the gut microbiota and participates in an international consortium to generate the gene catalogue of the porcine intestinal metagenome.

Main tasks in the proposal INRA will be involved in all WPs of the proposal. In the frame of WP1, INRA will be involved in tasks 1.4 and 1.5 and will lead task 1.7. INRA will contribute to the analysis of the sequencing data and will conduct the pilot project to assess whether the characterization of the porcine intestinal microbiota can be used to trace a given animal to its breeding system (e.g. food and/or environment). In WP2, INRA will be involved in tasks 2.1, 2.2 (task leader) and 2.3, with experts on assessment of environmental impact of animal production systems using various methods including Life Cycle Analysis, and on modelling of pig nutritional requirements. INRA will lead WP3 dealing with evaluation of quality of pork products and will be highly involved in tasks 3.1 (leader) and 3.2 with experts in meat science (especially pork) and animal science. INRA will participate in tasks 3.3 and 4.3 to conduct a case-study on consumers in France in close collaboration with IRTA/CREDA. INRA will participate in tasks 4.1 and 4.2 complementary to activities conducted within WP2, thanks to expertise on the multidimensional assessment of sustainability of pig production systems. In WP5, INRA will take part to a session at JRP (Journees de Recherche Porcines) in France, to a workshops during meetings of the Ligeral (herd book) members, to a presentation at Paris International Agricultural Show, to trade fair for food security in Toulouse “Sisqa” and “Tables du Rheu” in Rennes.

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Profile of the most relevant staff members who will be undertaking the work

Dr. Bénédicte Lebret is research scientist at INRA-PEGASE in the topic of meat science, working on influence of pig production systems on meat quality and development of biomarkers of meat quality. She had been head of research group Growth and Meat Quality at PEGASE for 10 years, and WP leader in FP6 Q-Porkchains research project. She is Deputy Section Editor of the international journal Animal. In this project she will lead WP3 and task 3.1 and participate in tasks 3.2, 3.3 and 4.3. Dr. Florence Garcia-Launay is research scientist at INRA-PEGASE in the topic of pig production systems modelling. She evaluates the impact of various pig production systems and practices on the environmental impacts (with Life Cycle Analysis) and the economic result of pig production. She is involved in various national projects dealing with Life Cycle Analysis of pig production, and pig production systems modelling. In this project she will be leader of task 2.2 and will participate in tasks 2.1, 4.1 and 4.2. Dr. Juliette Riquet has been head of the porcine molecular genetics group for 6 years and is now leading the molecular genetics and epigenetics team of GenPhySE. She has a 20 years-experience in linkage analysis, positional cloning of major genes, genetic diversity study, fine mapping of QTLs, high throughput genotyping and SNP analysis. She is a member of the scientific committee of the CRB-Anim (The national biological resources centre for animals) structure. In this project she will be involved in tasks 1.4 and 1.5. Dr. Jordi Estellé is research scientist at INRA-GABI and has extensive experience in molecular and computational biology applied to animal genetics and genomics and on the analysis of NGS data. His research activity targets the discovery of the genetics basis of the porcine immune response and its relation with intestinal microbiota. In addition, he contributes to the production of the first gene catalog of the pig gut microbiota by whole-metagenome sequencing. In this project he will be involved and lead task 1.7.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Damon M., Denieul K., Vincent A., Bonhomme N., Wyszynska-Koko J., Lebret B. 2013. Associations between muscle gene expression pattern and technological and sensory meat traits highlight new biomarkers for pork quality assessment. Meat Science, 95, 744-754. 2. Lebret B., Prunier A., Bonhomme N., Foury A., Mormède P., Dourmad J.Y. 2011. Physiological traits and meat quality of pigs as affected by genotype and housing system. Meat Science 88, 14-22. 3. Garcia-Launay F., van der Werf H.M.G., Nguyen T.T.H., Le Tutour L., Dourmad J.Y. 2014. Evaluation of the environmental implications of the use of amino acids in pig production using Life Cycle Assessment. Livestock Science, 161, 158-175. 4. Rousseau S., Iannuccelli N., Mercat M.J., Naylies C., Thouly J.C., Servin B., Milan D., Pailhoux E., Riquet J. 2013. A genome-wide association study points out the causal implication of SOX9 in the sex- reversal phenotype in XX pigs. PLoS One, 6;8(11):e79882. 5. Mach N., Berri M., Esquerré D., Chevaleyre C., Lemonnier G., Billon Y., Lepage P., Oswald I.P., Doré J., Rogel-Gaillard C., Estellé J. 2014. Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. PLoS One 9(2):e88515.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

- Q-Porkchains (integrated research project FP6, 2007-2012): Improving the quality of pork/products for consumer: development of an innovative, integrated and sustainable food production chain of high quality products matching consumer demands. Synergies with the present project are on i) environmental evaluation of pork production systems, ii) multidimensional assessment of sustainability of pork chains (including local chains), iii) sensory, healthy and technological evaluation of pork and pork products, using conventional and new (molecular biomarkers) methodologies. - Animal change (research project FP7, 2011-2015): An Integration of mitigation and adaptation options for sustainable livestock production under climate change. This project is connected to the present one through i) the evaluation of greenhouse gas (GHG) emissions of current livestock production systems and ii) the evaluation of innovative feeding and management practices that are identified as promising mitigation options for GUG emissions. Connections are both on research topic and methodologies. - DeliSus (national project ANR-07-GANI-0001): This integrated project aimed at studying the haplotypic variability of the porcine genome at a high density. The main breeds and related synthetic lines established by French breeders were analyzed for numerous phenotypic traits. The analysis of haplotypes was carried out for a very detailed analysis of genetic diversity of breeds and detection of selective sweeps revealing regions that had responded to selection. An association study was also carried out to estimate effect of

37 TREASURE 634476 haplotypes on all measured traits. Synergies with the present project are on i) diversity analysis, ii) genome structure and linkage disequilibrium analysis, and iii) genotype/phenotype association study analysis. - METALIT (INRA MEM metaprogramme 2012-2014, P.I. J. Estellé): This project targets the implementation of computational biology tools for de novo and quantitative metagenomics characterisation in livestock gastrointestinal tract datasets. Synergies with the present project are on data analysis with implementation of suitable statistical models to identify specific metagenomic characteristics related to pig production system.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

INRA-PEGASE (www6.rennes.inra.fr/pegase) is a joint research unit with Agrocampus Ouest and has strong participation in training in animal production. It includes experimental facilities and state of the art laboratories (biochemistry, molecular biology...). The INRA MEANS (MulticritEria AssessmeNt of Sustainability) platform, dedicated to the multicriteria analysis of the sustainability of animal and cropping systems, provides tools and references for research. The Centre for Genomic Resources (www.crb-gadie.fr) managed by INRA- GABI is a technological platform for the metagenomics activities providing a secured biobanking storage for porcine stool samples. The Get-PlaGe (http://get.genotoul.fr) is a core genomics facility linked to GenPhySE unit and provide state-of-the-art technologies for NGS-based genomics, transcriptomics and metagenomics (with 2 Illumina HiSeq2000, 1 Illumina MiSeq and 1 Roche 454 GS FLX+).

Partner 6 - Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH)

Description of the organisation

BESH is a farmers’ association founded in 1988. Its purpose is to market and promote the meat of the Schwäbisch Hällischen Schweine (SH). A reasonable market size is necessary to maintain the existence of this rare pig breed. The SH pigs are renowned for their very high meat quality. The legal standing of the BESH is a ‘wirtschaftlicher Verein, an organizational form comparable to a cooperative. The association consists of 1500 members. The Bäuerliche Erzeugergemeinschaft Schwäbisch Hall AG is one of BESH’s subsidiaries. It processes the meat. It has also an office for marketing activities which is responsible for selling and maintaining a market for the meat of the SH. The BESH group is an SME and owns a slaughterhouse with a capacity of 150.000 pigs, 50.000 piglets, 50.000 lambs and 15.000 cattle and organizes the slaughtering process as well as the meat processing. The BESH group runs six own shops in Schwäbisch Hall and Stuttgart and delivers meat and meat products to more than 400 butcheries and several hundred restaurants. The whole value chain from herd book management, pig breeding, farrowing, fattening, slaughtering, meat processing is managed by the BESH group as well as some flagship stores for consumers. The whole group is owned and managed by the farmers themselves. The BESH group holds certifications for Organic, Ecoland, Demeter, IFS and NON-GM Production. It is also involved in research about rare breeds and meat quality. The BESH arranged a protection from pork as Protected geographical Indication (PGI) in 1998. The Pork production follows mandatory regulations for animal welfare, feeding, pig keeping and nature conservation since 1988. In the last five years the BESH developed a model to keep pigs outdoor in the forest and on grassland. They are fed with acorns in their ratio according to traditional pig keeping models in the region Hohenlohe. BESH Member farms are running several pig pastures in the region. The BESH group is doing research about estimating organic breeding value from the pigs, meat quality and pig keeping systems under animal welfare conditions. It has established a system with individual identification from pigs with electronic eartags on several farms and an extended set of measures about meat quality with these pigs at the stage of the slaughtering. The marketing of the pork is another key competence from the BESH group. The BESH welcomes each year thousands of visitors and shows them farms, pig pastures, markets or the slaughterhouse from the BESH.

Main tasks in the proposal

WP1: Samples for genetic analyses. in WP2: Data/ sample collection; Evaluation of grass as feeding resource for Schwäbish Hällisches pigs

38 TREASURE 634476 in WP3: Expertise for development of toolbox. Evaluation of average and variability in quality of pork and pork products from Schwäbisch-Hällische Schwe. Influence of feeding resource (grass) on pork quality (connected with WP2 experiment). Participation in development of database on systematic determinations of carcass and meat quality traits, for further implementation of breeding programs. in WP5: organization of breeders meeting in Germany, development of TREASURE project and demonstration activities for farmers and web and media dissemination.

Profile of the most relevant staff members who will be undertaking the work

Rudolf Bühler, Dipl. Ing. Agr. Senior Expert in rural development, Head and founder from the BESH and several other organizations. International project Development, Member of the EU Focus group “Genetic Resources” Christoph Zimmer, MBA Expert in pig keeping, Meat quality research, CEO from Ecoland e.V., Executive Board Member “Bund ökologischer Lebensmittelwirtschaft”, Executive Board Member “Verband Lebensmittel ohne Gentechnik” Fritz Wolf, Dipl. Ing. Agr. Farmers consultant, international project development, expert for animal welfare pig keeping systems, project developer pig pastures

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content 1. "Solidaric Business and Further Core Values in Sustainable Rural Development Projects". Key Note Speach an der Universität Oxford, Worcester College, 8th Congress on Green Economics. Oxford, 18.-19. 7. 2013. 2. Bedeutung Alter Landrassen im Ökologischen Landbau", Vortrag an der Uni Hohenheim, AK Ökologischer Landbau, Hörsaal 20, 21.1. 2013, 3. "Alte Rassen in Wert setzen - Erhaltungsstrategien für bedrohte Haustierrassen am Beispiel der Wiederbelegung des Schwäbisch-Hällischen Landschweins". Vortrag anl. der 5. Wintertagung Ökologischer Landbau Baden-Württemberg, Schloss Hohenheim / Universität Hohenheim, 14.3. 2012 4. "Methods and Strategies for Revitalization of Endangered Breeds - Proposal of a PPP Project for the Conservation of the Jinhua Pig Breed in China". Vortrag an der Zhejiang Jinhua Pig Breeding Farm, Jinhua, Provinz Zhejiang, China, 08.01. 2011 5. "Value Chain Development in the Agricultural Sector - Case Study of Farmers Association of Schwäbisch Hall" Vortrag anlässlich EXPO 2009, GTZ-Forum on Value Chain Development, Hefei, China, 26./27. April 2009.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal  Management Pilot 7: Implementation of regional pork chain concepts and new pork product concepts – Q-PorkChains (EU FP6 Project)  Management Pilot 5: Implementation of sustainability aspects in the quality pork program of a regional chain Q-PorkChains (EU FP6 Project)  Projekt “Feldprüfung Schwäbisch-Hällisches Schwein” – KF 0414801 MD6; in cooperation with Justus von Liebig University, Gießen, founded by Bundesministeriums für Wirtschaft und Technologie (BWMi) via Reserach Program “Pro Inno II”  "Ökologischer Gesamtzuchtwert Schwäbisch-Hällisches Schwein" Research Project ÖZW-SHS 020E396, founded by Bundesdesprogramm ökologischer Landbau, R.Bühler/G.Postler, 2005

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Own slaughterhouse with the possibility to take apart animals Laboratory for meat quality measurement (e.g. shear force, intramuscular fat content, opto, drip loss) Herd book breeding farms, farrowing farms and fattening farms – connected by individual registration from pigs with electronic ear tags

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Partner 7 - SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG)

Description of the organization

The University of Zagreb (UNIZG), Faculty of Agriculture (founded in 1919) is the leading institution in the Republic of Croatia, with respect to the education and researching in the field of agricultural and related sciences and the profession. It involves over 200 researchers in scientific and research work and over 12000 Engineers, Masters and Doctors of Science degrees to date. Our teachers and researchers are involved in implementation of basic, development and applied research projects, and actively participating in professional collaboration. The Faculty organization is based on 28 departments. Within departments there are 28 laboratories and 6 experimental stations intended for teaching, and scientific and technical activities. Two departments mainly engaged in the implementation of current proposal are Department of animal science, which has broad experience in animal breeding and genetics, biostatistics, meat science and animal health, and Department of marketing in agriculture, which is main research unit dealing with marketing of agricultural products and food, including consumer research and traditional food products.

Main tasks in the proposal

The UNIZG with its experience will be involved in phenotypic characterization of two local Croatian pig breeds: Black slavonian (BS) and Turopolje pig (TP) providing various data (anatomical, metabolic, production, carcass traits, meat quality etc.) and samples (DNA, faeces etc.). We will perform a dietary experiment with natural foodstuff (pasture, acorn) for pig feeding and will participate in development of high quality meat products from our local BS and/or TS pig breeds. Additionally, UNIZG with its experience in the area of agricultural and traditional food marketing will be in involved in consumer acceptability studies, by development of consumer research concept, methodology and guidelines, creation and testing of data collection tools, empirical data collection, analyses and evaluation of collected data. Finally, we will perform consumer preferences and WTP studies related to new products from our local pig breeds. As a partner we will be involved in project dissemination and demonstration activities (e.g. publishing papers, presentation of project results on national/international symposiums, workshops with local pig breeders etc.) like the workshops for farmers/meat processors with the support of our associated third party.

Profile of the most relevant staff members who will be undertaking the work

Dr. Danijel Karolyi works 16 years at the Department of animal science. He completed his graduation in 1998, MSc in 2002 and Ph.D in Animal science in 2007 (UNIZG). He is associated professor and lecturer on at several animal or meat science modules on all levels of university studies. His main research area concerns meat and meat products quality. He worked on several national and international projects, including two EUREKA projects (E!3114-AFA, E! 3983-RFA). Dr. Marija Cerjak works 15 years at the Department of agricultural marketing. She graduated (1998), completed his MSc (2001) and Ph.D (2005) in Agroeconomy (UNIZG). She is associated professor and lecturer on the subject of agricultural marketing. She worked on several national and international projects (e.g. FP6 - 513705, FP7 – 211760) as project leader or collaborator. Her main research area is food related consumer behavior. Dr. Krešimir Salajpal works at the Department of animal science as an associated professor. His main research area is animal physiology, stress response and health. Dr. Zoran Luković works at the Department of animal science as an associated professor. His main research area is pig production and breeding.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Škorput D., Gorjanc G., Đikić M., Luković, Z. (2014): Genetic parameters for litter size in Black Slavonian pigs. Spanish journal of agricultural research, 12: 89-97 2. Karolyi D., Rimac D., Salajpal K., Kljak, K., Štoković I. (2012): The influence of dietary linseed on alpha- linolenic acid and its longer-chain n-3 metabolites content in pork and back fat. Veterinarski Arhiv, 82: 327-339

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3. Druml T., Salajpal K., Dikic M., Urosevic M., Grilz-Seger G., Baumung R. (2012): Genetic diversity, population structure and subdivision of local Balkan pig breeds in Austria, Croatia, Serbia and Bosnia-Herzegovina and its practical value in conservation programs. Genetics Selection Evolution 2012, 44:5 4. Karolyi D., Salajpal K., Luković Z., Kovačić D. (2012): Physicochemical, hygienic and organoleptic characterisation of Slavonian Kulen - a traditional pork sausage from eastern Croatia. Options Mediterraneennes Series A: Mediterranean Seminars 2012 - Number 101, 281-288 5. Cerjak, Marija; Karolyi, Danijel; Kovačić, Damir (2011): Effect of information about pig breed on consumers' acceptability of dry sausage, Journal of sensory studies, 26: 128-134

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

2001-2005 Slavonian kulen technology and development project (Ministry of science) 2003 - 2006 Influence of animal diet on fatty acid composition of pork (EUREKA Project E!3114-AFA); 2006 - 2007 Alternative pork production (Ministry of agriculture) 2006-2008 Drniš ham - product specification for PGI (Ministry of agriculture) 2012 Slavonian kulen - product specification for PGI (Ministry of agriculture)

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Technical equipment for meat and meat products quality measurement (e.g. chromamete MINOLTA CR-410, Rotronic Hygropalm, Testo 220 pH meter), Laboratory for chemical analytics of food/feed, Laboratory for molecular genetics analyses Partner 8 - SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEK (PFOS)

Description of the organisation Faculty of Agriculture in Osijek is part of the J.J. Strossmayer University of Osijek and is one of its oldest members. 18. October 2010 marked exactly half a century of its foundation. Besides teaching students at undergraduate, graduate and postgraduate studies, the major activity of the Faculty is scientific research, primarily in the field of Agriculture and Biotechnology. The mission of the Faculty is development and systematic improvement of dynamic and multidisciplinary research environment in which research potentials will be exploited in maximum and creation of new and improvement of existing knowledge is a constant. These research environment provide excellent learning process and transfer up to date knowledge and skills to our students and enable them to become leading agricultural experts and to apply new knowledge in everyday production practice. Faculty with its research environment, education of agricultural experts and continuous development and skills needed for life long education serves to the community and contributes to the development of society on the whole.

Main tasks in the proposal

Faculty of Agriculture in Osijek will take part in the activities within following Work Packages (WP): WP1 - Description and evaluation of local pig genetic resources, WP2 - Management and performance of local pig breeds in their production systems, WP3 - Traditional and new high quality products with regional identity and WP5 – Dissemination and Demonstration. The researchers involved in the project proposal are skilled in the investigation of genetic and environmental influences on carcass and meat quality traits of pigs, including our own indigenous pig breed – Black Slavonian. In their line of work they studied the use of molecular markers in the prediction of carcass and meat quality traits such as RYR1, CAST, PRKAG-3, MC4R, H-FABP3 and LEPR. Currently they are involved in the research of influence of gender (including entire-males) and breed on boar taint expression in pigs. They are also experienced in the research of product quality including raw meat, traditional fermented sausages etc. Main emphasis in these investigations is the use of Black Slavonian pig as the basis for production of kulen (most appreciated fermented sausage in Croatia). In WP5 activities, PFOS will be involved particularly in the organization of an international contest of fermented sausages (»Sausage Olympics«), to be held in east

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Croatia (Slavonia region) and in a workshop for farmers/meat processors with the support of his associated third party, LETA.

Profile of the most relevant staff members who will be undertaking the work

Prof.dr.sc. Goran Kušec; experienced in evaluation of meat quality in laboratory and at slaughter house, knowledge in determination of lean content in pig carcasses, DNA isolation from different tissues and genotyping by RFLP-PCR.

Doc.dr.sc. Vladimir Margeta; genetic analysis of Black Slavonian pig breed, also involved in study of different keeping conditions which are used in today pig production.

Doc.dr.sc. Ivona Kušec Djurkin; experienced in evaluation of meat quality in laboratory and at slaughter house, DNA isolation from different tissues and genotyping by RFLP-PCR. Kristina Budimir, mag.ing.agr.; main occupation is preservation of autochthonous pig breeds and also investigation of genetic structure of Black Slavonian, i.e. Croatian autochtonuos pig breed. Boris Lukić, dipl.ing; genomic prediction of carcass and meat quality traits of pigs as well as various other ways to improve the quality of animal products.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Kušec G., Baulain U., Henning M., Köhler P., Kallweit E. (2005): Fattening, carcass and meat quality traits of hybrid pigs as influenced by MHS genotype and feeding systems. Archiv für Tierzucht, 48(1):40-49. 2. Kušec G., Kralik G., Horvat D., Petričević A., Margeta V. (2005): Differentiation of pork longissimus muscle regarding the variation in water holding capacity and correlated traits. Proceedings of the 13th International Symposium «Animal Science Days», Agripolis, Padova, 12-15.09.2005., Italian Journal of Animal Science, 4(3):79-81. 3. Kušec, G., Baulain, U., Kallweit, E., Glodek P. (2007): Influence of MHS genotype and feeding regime on allometric and temporal growth of pigs assessed by magnetic resonance imaging. Livestock Science 110(1- 2):89-100. 4. Kristina Budimir, Vladimir Margeta, Polona Margeta (2013): Breeding of the black slavonian pig in extensive conditions. Acta argiculturae Slovenica. 4: 93-96. 5. Polona Margeta, Vladimir Margeta, Kristina Budimir (2013): How black is really Black Slavonian pig?. Acta argiculturae Slovenica. 4: 25-28.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

 2006 – 2014. Early prediction of pig carcass and meat quality – Project funded by Croatian Ministry of Science and Education, Principal: Goran Kušec  2012-2014. Application of molecular genetics to improve the breeding of Black Slavonian pig – Project funded by Croatian Ministry of Agriculture, Principal: Vladimir Margeta  2012-2014. Improvement and standardization of quality of Kulen in family farms in Baranja – Project funded by Croatian Ministry of Agriculture, Principal: Goran Kušec  2012-2014. Determination of pure Black Slavonian pig using molecular genetic method – Project funded by Osijek-Baranja County, Principal: Vladimir Margeta  2013 – Today. DNA identification of meat of different animal species in traditional meat products – Project funded by Josip Juraj Strossmayer University of Osijek, Principal: Ivona Đurkin

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Faculty of Agriculture in Osijek is equipped for the basic DNA investigations: DNA isolation, PCR, electrophoresis as well as for the measurement of main meat quality traits: pH-meter, Minolta CR-300, TA.XTplus Texture Analyser, EZ-drip, Grau and Hamm water holding capacity, etc. At the Faculty of Agriculture it is also possible to analyse chemical composition of the meat and meat products, including minerals such as Fe, Zn, Se, etc.

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Partner 9 - AGRIS Sardegna (AGRIS)

Description of the organization Agris Sardegna (Regional Agricultural Research Agency: ref. Sebastiano Porcu, Roberta Comunian) has been founded to carry out research, trials, technological innovation aimed at: promote sustainable development of agricultural, agro-industrial, forest and fish resources sectors; contribute to protection and enhancement of animal, plant and microbial biodiversity; increase competitive skills in scientific research. The main sectors of activities are: Zootechnical Division (Animal Feeding and Nutrition, Farming Systems, Genetics and Biotechnology, Reproduction, Experimental farms management), and Animal Origin Products Division (Microbiology, Chemistry, Technology, and Product Enhancement and Control)

Main tasks in the proposal We will contribute on the following points included in the WPs listed below: WP1. Description and evaluation of genetic resources - phenotypic description of genetic resources of Sarda breed pig WP 5: Dissemination and development A good practice guide on the autochthonous breed pig rearing and feeding systems and meat processing will be developed, in collaboration with local farmers’ associations and producers, in order to harmonize the traditional pork meat products processing. Organisation of training course for Sarda pig breeders and meat processors and workshop with TREASURE project results. Moreover, the dissemination and demonstration of project outcomes by workshops and training courses will be carried out.

Profile of the most relevant staff members who will be undertaking the work Sebastiano Porcu Area of expertise: Zootechnician skilled in Sarda breed pig rearing and traditional Sardinian meat products characterisation. Work experience: Technician in charge of one of the experimental farms of the Agris Sardegna Animal Production Department (DiRPA), where cattle, goats and pigs are reared. He is also in charge of the meat products pilot plant of DiRPA. He has been involved in research studies aimed at the Sarda breed pig characterisation and its official recognition (D.M. 21664, 8th of June 2006). Main activities and responsibilities: Coordination of the activities carried out at the experimental farm and the pilot plant for the production of cured meat. Evaluation of morphological characteristics of swine reared in various Sardinian farm, aimed at detecting individuals having the appropriate characteristics to be included in the official Register of traditional population. Drafting and implementing of scientific projects in collaboration with other sectors of the department, national and European organizations and universities. Writing reports and scientific papers. Participation in conferences, meetings and seminars. Member of working groups/Technical Committee for the protection of Sarda breed pig and the improvement of the quality of meat products. Teaching activities in the context of training courses for technicians working in the swine sector. Teaching at the School of Specialization in Inspection of Food of Animal Origin at the Department of Veterinary Medicine - University of Sassari.

Roberta Comunian Area of expertise: Food microbiology, in particular lactic acid bacteria colonizing animal and vegetable fermented products. Work experience: Researcher in charge of the Microbiology Laboratory at the Animal Production Department (DiRPA) of Agris Sardegna Agricultural Research Agency of Sardinia Main activities and responsibilities Coordination of the Microbiology laboratory activities. Drafting and implementing of scientific projects in collaboration with other sectors of the department, national and European organizations and universities. Writing reports and scientific papers in Italian and English. Participation in conferences, meetings and seminars. Member of working groups/Technical Committee for the improvement of the quality of dairy traditional and PDO cheeses. Teaching activities in the context of training courses for technicians working in the ewes and goats dairy sector. Teaching of dairy Microbiology at the School of Specialization in Inspection of Food of Animal Origin at the Department of Veterinary Medicine - University of Sassari. Peer Review activity for some international journals.

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List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content 1. S. Porcu, R. Mazzette, C. Diaferia, G. Riu, C. Manca, M. Addis, 2013, Qualitative, chemical and nutritional traits of dry-cured ham of sarda breed pigs: effect rearing system. 8th International Symposium on the Mediterranean Pig, October 10th-12th, 2013, Lubiana, Acta Agriculturae Slovenica, Suppl. 4: 145-149 2. S. Porcu, M. Decandia, S. Pintus, P. N. Lei, M. A. Sanna, S. Ligios. Effect of feeding and rearing system on growth performance of Sarda breed pig: preliminary study. 7th International Symposium on the Mediterranean Pig, October 14th-16th, 2010, Cordoba, Andalusia, Spain. Options méditerranéennes, Series A n.101, 2012, 367-371; 3. S. Porcu, R. Mazzette, C. Manca, M. Decandia, G. Busia, G. Riu, S. Ligios. Biometric and rheologic parameters and qualitative properties of meat from ”Sarda” breed pigs: preliminary results. 7th International Symposium on the Mediterranean Pig, October 14th-16th, 2010, Cordoba, Andalusia, Spain. Options méditerranéennes, Series A n.101, 2012, 373-377; 4. S. Porcu, R. Mazzette, C. Manca, M. Decandia, D. Meloni, S. Ligios. Effects of feeding and rearing system on carcass characteristics of Sarda breed pig: preliminary study. 7th International Symposium on the Mediterranean Pig, October 14th-16th, 2010, Cordoba, Andalusia, Spain. Options méditerranéennes, Series A n.101, 2012, 379-382 5. Daga E., Mannu L., Porcu S., Comunian R., Paba A., Scintu M.F., 2007. Home-made dry sausages produced in Sardinia: an investigation on the microflora. Italian Journal of Food Science, 19(3), 297-308.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal 1. Project “Realizzazione di un allevamento sperimentale di suini di razza Sarda, finalizzato allo studio e alla salvaguardia del suino di Tipo Genetico Autoctono (TGA), alla caratterizzazione e alla valorizzazione dei prodotti tipici della salumeria tradizionale della Sardegna” (“Implementation of an experimental breeding of Sarda breed pigs, aimed at the study and preservation of the Sarda breed pig, the characterization and the enhancement of the traditional typical cured meat products of Sardinia" funded by the Assessorato dell’Agricoltura e Riforma Agro-Pastorale Regione Sardegna (Department of Agriculture of the Regional Government of Sardinia); 2. Project “Valorizzazione della filiera del suino di razza Sarda” (“Enhancement of the Sarda breed pig chain”) funded (€ 193,000.00) by the Assessorato dell’Agricoltura e Riforma Agro-Pastorale Regione Sardegna (Department of Agriculture of the Regional Government of Sardinia) 3. RISGENSAR Project “Conservazione delle risorse genetiche animali e vegetali di interesse agrario” (“Preservation of animals and plants of agricultural interest genetic resources”) funded by PSR Regional Government of Sardinia.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work Genetics and biotechnology laboratory: Thermal cyclers, Genetic Analizer for DNA sequencing, Real-Time PCR System Chemistry laboratory: GC, HPLC, NIR System, Spectrophotometer, Atomic absorption spectroscopy Microbiology laboratory: Microbiological analyses basic equipments, Thermal cyclers, DGGE System, PFGE apparatus, Ultrafreezers -80°C, microbial cultures collection Meat products pilot plant: Experimental farm in wood area for pigs rearing (40 hectars)

Partner 10 - Associazione Nazionale Allevatori Suini (ANAS)

Description of the organisation

Associazione Nazionale Allevatori Suini (ANAS), is a non-profit organization founded with the name of "Associazione Nazionale Allevatori di suini di razza Large White" in June 12, 1957 and legally recognized with D.P.R. December 23, 1962 n. 2031, published in Official Journal of the Italian Republic on 15 March 1963 n. 72. The Association changed its name to "Associazione Nazionale Allevatori Suini " on 10 December 1963. ANAS promotes and implements initiative that can contribute to the improvement, development and promotion of pigs and their products. ANAS, under the supervision of the Italian Ministry of Agriculture, is

44 TREASURE 634476 responsible for maintaining the Italian Herd Book of purebred pigs (Italian Large White, Italian Landrace, Italian Duroc, Pietrain) and the Register of minor breeds (as Belgian Landrace, Hampshire and Spot and the autochthonous pigs as Cinta Senese, Mora Romagnola, Nero Siciliano, Casertana Apulo- Calabrese, Sarda). ANAS is also responsible for the National Register of all Registers of hybrid pigs operating in Italy. These functions of ANAS are carried out in compliance with specific rules approved by the Italian Ministry of Agriculture.

Main tasks in the proposal

ANAS will contribute to the activities for the phenotyping of pigs in the testing station of Gualtieri and in dissemination activities (WP5) – publications and participation on meetings.

Profile of the most relevant staff members who will be undertaking the work

ANAS Director, Maurizio Gallo has a degree in Agricultural Science, works at ANAS since 1988 and he is ANAS Director since 2000. Mr. Gallo collaborates on the definition and management of the selection scheme of pig breeds. He organizes and manages the Italian Herd Book and the Register of minor breeds and the National Register of Registers of hybrid pigs. He also represents ANAS at EPSPA (European Pig Selection and Production Association) and EFFAB (European Forum of Farm Animal Breeders).

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Centro Genetico - Via D'Este,6 - 42044 S. VITTORIA DI GUALTIERI (Reggio Emilia) ANAS is responsible for the management of the testing station of Gualtieri and of the processing of the collected data. The tests performed in the testing station establish the genetic value of a candidate boar: for each candidate young boar, a group of three full sibs (2 females and 1 castrated male) is controlled on station. The breeding scheme implemented by Italian Herd Book maintained by ANAS improves the efficiency of the production of pork suitable for processing typical Italian hams. This program concerns three populations: Italian Large White, Landrace and Duroc.

Partner 11 - UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI)

Description of the organisation

The Department of Agrifood Production and Environmental Sciences (DISPAA) is representative of the following undergraduate degrees: Agricultural Sciences and Wildlife Sciences, and of the following graduate degrees: Agricultural Science and Technology, Rural Development, Wildlife Management, Tropical Rural Development. DISPAA is the administrative headquarters of the School of Agriculture and of the PhD in Agricultural and Environmental Sciences. The scientific activity of the Animal Science section of DISPAA (AS-UIFI), involved in the project, mainly concerns: - productive and reproductive performance and genetic improvement of farm animals; - conservation, dissemination and use of autochthonous animal germplasm; - biotechnology applied to livestock production; - animal nutrition and evaluation of animal feed; - breeding and management of domestic farm animals and wildlife and their interactions with the territory; - aquaculture management; - animal welfare; - characterization, evaluation and traceability of animal products, fresh or processed. The AS-UNIFI has, since 1991, conducted research on Cinta Senese pig breed facing all the aspects related to the conservation and exploitation of local genetic resources with particular regard to inbreeding control, nutritional requirements, quality of fresh meat and seasoned products, cooperation among suppliers and between suppliers and retailers in the food chain, and the development of specific brands. AS-UNIFI has considerable expertise in animal genetics and meat quality. AS-UNIFI has published widely and has numerous collaborative projects with farmers and industry. AS-UNIFI is comprised of 14 researchers plus technical staff.

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Main tasks in the proposal

WP1 –AS-UNIFI developed researches on phenotypic and genetic characterization of livestock breeds (mainly cattle, sheep and pigs) facing aspects related to pedigree data, morphological and production data. The expertise is focussed on local breeds with special emphasis on the needs of reduced size populations. WP2 – AS-UNIFI has been involved since the early nineties in the recovery and valorisation of Cinta Senese pig breed and numerous study have been carried out on-farm data as well as on nutritional requirements given special attention to the energy/protein balance in unimproved breeds. WP3 – AS-UNIFI has a wide experience on meat quality assessment of cattle and pigs and in these last 20 years has focussed the research activities on transformed and cured products from pig meats. WP4 – AS-UNIFI has a deep knowledge of the realities of the Cinta Senese pig breed and of trends in the market for its products. In this WP the contribution of UNIFI will be essential for the analysis of cost-benefits and consumer preferences. WP5 – AS-UNIFI will be involved in publication, participation on meetings and in the organization of "consumer orientation" courses.

Profile of the most relevant staff members who will be undertaking the work

Carolina Pugliese Gender: Female Education: Researcher since January 2004. PhD in Animal Sciences in 1998. Scientific Activity: The research topics involved: study on meat quality in several species as affected by genetic, feeding, rearing and technological factors; use of the NIRS technique for the estimation of meat quality, pig feeding, with particular regards on comprehension of the digestive physiology by means of in vivo digestibility trials; management of small populations; biodiversity and genetic conservation of indigenous breeds; sustainable farming. External reviewer in three European doctoral thesis. Publications by 30 May 2014: Total No.: 127 Books and books chapters: 7 IF 31.6 SCOPUS: 37 publications, 239 total citations, 9 h index Riccardo Bozzi Gender: Male Education: Associate Professor since 2006. Ph.D. Degree "Agrobiotechnology for the Tropical Production" obtained at the University of Florence in 1994. Research activity is especially focused on: animal breeding of cattle, sheep and swine; management of population; conservation and exploitation of autochthonous breeds; genetic variability; molecular traceability methods. National representative of the COST project “Optimising and standardising non-destructive imaging and spectroscopic methods to improve the determination of body composition and meat quality in farm animals” (FAIM 2012-2015). Scientific Activity: Publications by May 30th, 2014: Total No.: 226Books and books chapters: 14 IF 35.3 SCOPUS: 44 publications, 268 total citations, 10 h index

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. PUGLIESE C., SIRTORI F. (2012). Quality of meat and meat products produced from southern European pig breeds. Review Article. Meat Science, 90, 511-518.(IF = 2.619). 2. PUGLIESE, C., SIRTORI, F., ACCIAIOLI, A., BOZZI, R., CAMPODONI, G., FRANCI O. (2013). Quality of fresh and seasoned fat of Cinta Senese pigs as affected by fattening with chestnut. Meat Science, 93, 92-97. (IF = 2.619). 3. PUGLIESE C., SIRTORI F., CALAMAI L., FRANCI O. (2010). The evolution of volatile compounds profile of ‘‘Toscano’’ dry-cured ham during ripening as revealed by SPME-GC-MS approach. Journal of Mass Spectrometry, 45, 1056-1064. (IF = 3.153) 4. ACCIAIOLI A., SIRTORI A., PIANACCIOLI L., CAMPODONI G., PUGLIESE C., BOZZI R., FRANCI O. (2011). Total tract digestibility and nitrogen balance of diets with different protein contents in Cinta Senese pig. Animal Feed Science and Technology, 169, 134-139 (IF = 1.720) 5. BOZZI R., ALVAREZ I., CROVETTI A., FERNANDEZ I., DE PETRIS D., GOYACHE F. (2012). Assessing priorities for conservation in Tuscan cattle breeds using microsatellites. Animal 6, 2, 203–211. doi:10.1017/S1751731111001443.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal • "Characterization of salami of Cinta Senese pigs as a function of rearing system" Funded by University of Florence. (2005-2006).

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• "Characterization of PDO Toscano dry-cured ham as a function of aromatic composition” Funded by Tuscany Region. (2008-2009). • “Qualitative characterization of Slovenian dry-cured ham Kraški pršut”. Funded by the Slovenian Ministry of Research (2012). • "Innovative Techniques for the production of the "Cuore di spalla " salami". Funded by Tuscany Region. (2013). • "Optimising and standardising non-destructive imaging and spectroscopic methods to improve the determination of body composition and meat quality in farm animals". COST project (2012-2015).

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

The DISPAA provides the following facilities: - Experimental stable with six metabolic cages with various room laboratories; - chemical, biological and rheological laboratories equipped to perform the following analysis: Gas- Chromatography (GC), High-Performance Liquid Chromatography (HPLC), Near-infrared spectroscopy (NIRs), Texturometer Analysis Profile (TPA) DISPAA is a part of the following research centres: - Mass Spectrometry Centre (CISM) - Interdepartmental Research Centre for Food Valorisation (CERA) - Interdepartmental Service Centre for Biotechnologies (CIBIACI)

Partner 12 - Centri Regionali per le Tecnologie Agroalimentari (Ce.R.T.A. scarl) (CERTA)

Description of the organisation

CeRTA is a network of expertise and services in the food technology field. It comprises more than 70 laboratories distributed throughout southern Italy. CeRTA supports companies in the agrifood-related life sciences field, with the goal of providing services for successful increased competitiveness and innovation, promoting the application of research results in enterprises. CeRTA prerogative of is to act as an intermediary between Research and Enterprise, in order to generate innovation processes making less expensive technology transfer interventions, through the expertise in fundraising, the sharing of homogeneous innovation needs and the resulting distribution of costs, using the related resource critical mass. CeRTA activity aims at encouraging and facilitating the emerging of innovative needs, translating this needs in real business improvement projects and providing skills, knowledge and resources to solve business problems. Thanks to its multidisciplinary staff and its capillary distribution, CeRTA is able to offer various types of services, being able to support Agrifood companies, in: SPREADING technological and scientific informations aimed at: • identification of innovative technology solutions for business; • information on the national and European technical regulations; • information on the procedures for obtaining patents and trademarks; • information and access procedures for community, national and regional development and innovation programs. TECHNOLOGY TRANSFER through: • identification of external Centers of technological expertise for the external needs of businesses assisted; • provision of integrated services and technical-scientific equipment; • coaching companies in the identification and implementation of innovative customized choices. PROMOTION of new business activities characterized by high-profile science and technology. INSTITUTION of courses and training fellowships for high specialization. PARTICIPATION to European, national and regional projects.

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Main tasks in the proposal

CeRTA in Treasure will have a role in administrative support and in dissemination and demonstration workpackage WP5. For administrative function, CeRTA has 5 years-experience in management of public – private R&I projects, since in its mission it is a center of technological expertise created by the Italian Ministry of University and Research for the promotion of scientific-technological development of enterprises (in particular SMEs). The centers act as intermediaries for research and innovation and, through the provision of services in scientific and technological content, work to promote and support both the strengthening of the links between science and business systems and the collaboration between the SMEs, in order to raise the propensity for innovation of the production system in the concerned regions. For dissemination, CeRTA has in its mission to promote the application of research results in companies (in particular SMEs) in the field of life sciences related to Agrifood. Even more, CeRTA was born to act as technology transfer intermediary for finding solutions to technological acceleration able to promote the growth of enterprises, through the identification of technological competence centers. In details, CeRTA will be coordinator of all WP5 activities: Task 5.1 – publications and organization of meetings, with the development of brochures and leaflets for dissemination events, and the active project networking Task 5.2 – development of a new collective trademark, coordinating the development and registration of the collective trademark TREASURE Task 5.3 – web and media dissemination, coordinating and developing the web platform for the project dissemination Task 5.4 – coordinating the transfer of knowledge activities

Profile of the most relevant staff members who will be undertaking the work

Loreto GESUALDO (1960, Male) Current position at CeRTA: President Prof. Gesualdo is Full Professor of Nephrology at University of Bari. He has a consolidate management experience, since he fills the role of CeRTA's president since five years. Moreover, he has been the former President of DARe (Apulian Agrifood Technology District) and former Director of the Interdepartmental Centre for Research and Biotechnological Applications for Security and Promotion of Local Products and Quality (BIOAGROMED), University of Foggia He is currently Member of the Board of Directors of the District H-BIO Puglia Scrl - Technological District Pugliese's Health and Biotechnology . He is also a peer reviewer for 15 international journals and is on the editorial boards of five international journals, as well as being the author of 381 papers in reviewed journals, with more than 5,144 citations, an impact factor greater than 1,000 and an H-index = 38.

Giuseppe DALFINO (1980, Male) Current position at CeRTA: Technical Director Dr. Dalfino is a Plant Biotechnologist, with a PhD in Medical Biotechnology. He has experience in project management as he coordinates many projects of research and development, funded by the Italian Ministry or regional authorities, in partnership with universities and SMEs, on behalf of CeRTA and University of Bari. Currently, Dr. Dalfino, acting as Technical Director of the CeRTA, supervise a technology platform worthy over € 6 million, branched over a wide territory multiregional (Apulia, Basilicata, Calabria, Campania, Sicily and Sardinia), coordinating all corporate ordinary activities, such as, for example, participation in invitations to address the technical-scientific and related project management, and requirements of such statutory, fiscal, administrative and accounting. His scientific activity is focused on Food and Health.

Carmela COSOLA (1981, Female) Current position at CeRTA: collaborator expert in project management Dr. Cosola is a Biologist with a PhD in Medical Biotechnology. She has experience in basic and translational research, dealing with health biotechnology with the University of Bari. She has specializations and expertise in quality, environmental and food safety management systems, and in food law compliance and quality certifications consulting.

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List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

• CeRTA is involved in 18 projects under the notice issued by Decree of the Director of the Sicilian Region n.600/2009 which triggered the Measure 1.2.4 . the Rural Development Programme 2007 - 2013 of the Sicilian Region "Cooperation for development of new products, processes and technologies in the agriculture and food sector and in the forestry sector" (approved by the European Commission Decision No . EC (2008) 735 of 18 / 02 /2008). That measure, which finances the transfer, verification and testing innovation aimed at increasing the competitiveness of enterprises. The objective of the project is to promote modernization and innovation in business and the integration of the supply chain through collaboration and cooperation between primary producers ( individual or combined ) , business processing and marketing companies providing tools, technologies and services , public and private institutions operating in the field of pre-competitive research and experimentation. CeRTA is involved in training and mentoring offered by subjects of the scientific world to increase the professional skills of human resources. • Living Labs Smart Puglia 2020 – “HelpLarge” project: Apulian partnership that offers innovative semantic aimed to promote wellbeing and healthy eating for the social inclusion and active aging and healthy. Innovative methodology applied: Participatory Design. The citizens are actively involved in all stages of design, development and implementation of research products. • Savegrainpuglia: recovery, characterization, preservation and enhancement of legumes and cereal crops and forage in Apulia. • LOGIN: funded by the Ministry of Productive Development with the initiatives INDUSTRY 2015 "New Technologies for the Made in Italy", is a project aimed to set-up an innovative integrated logistic platform.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

CeRTA has 50 branches and laboratories active in the Italian territory of competence, dedicated to technology transfer and supporting SMEs willing to activate innovation processes in the Agrifood field.

Partner 13 - ALMA MATER STUDIORUM-UNIVERSITA DI BOLOGNA (UNIBO)

Description of the organisation

The Alma Mater Studiorum University of Bologna, is the oldest university in Western Europe and one of the most important institutions of higher education across Europe with 11 Schools, 33 Departments and about 87.000 students. The University places special emphasis on the excellence in education and research. Strengthening the internal network, creating interdisciplinary critical masses of scientists is one the major goals for UNIBO. The University has 7 Interdepartmental Centers dedicated to industrial research In particular Alma Food IRT is a unique access point to multi-disciplinary expertise related to the agro-food field and KBBE areas, organized in thematic pillars for a quick and easy approach. It is a reference point and a main partner at both national and international level. Alma Sequencing IRT integrates the competence and capabilities in the field of next generation sequencing (NGS), providing know-how stretching from life sciences to ethics, systems biology to environment, metagenomics to bioinformatics and high scale computing. At National level UNIBO ranks second for competitive research funding from the Italian Government. On the European side, UNIBO is member of the major European Networks and stakeholders thematic groups, such as the European and National Technology Platforms. Nowadays UNIBO counts 11,000 products research (annual average) and 200 patents in the portfolio and has obtained 91 million Euros from the Seventh Framework Programme and other programs financed by the EU (from 2007 to September, 2013). Today, the Alma Mater Studiorum is the first Italian university for the attractiveness of European funding for research (projects funded in 2007-2012), 31st in the European ranking of institutions of higher education. In particular,

49 TREASURE 634476 referring to the FP7 Cooperation program - KBBE theme projects, UNIBO has been involved in 50 successful proposals, coordinating 9 of these: the first University in Italy and among the top ten European players for European funding in the agro-food field. Referring to Horizon 2020 and related European initiatives, the University of Bologna is active within the European Innovation Partnerships; is involved as a partner in several JPIs’ projects and coordinates the Italian DEDIPAC Hub within the JPI Healthy Diet for Healthy Life. The University of Bologna is also participating to the Knowledge and Innovation Communities: in particular, in the Food&Bioeconomy area, UNIBO is affiliate member in the Climate-KIC and is a member of the Foodbest Consortium working, on a KIC-Food proposal, for an Italian Co-location Center.

Bologna University is organized in Departments. The Department of Agricultural and Food Science and Technology (DISTAL) is involved in this project and it is organised in seven areas of researches. One of these areas is the “Animal Production” to whom the scientists involved in the proposal belong. The department of DISTAL has a long story of genetic and genomic analyses at all levels with both wet and dry laboratories with strong experiences in several species and special concern for pigs. Researches are carried out for all levels of the pig meat production chain from the animals, and their breeding, to the market with special attention to quality products. Finally, the department is directly involved in the inter-departmental laboratory “Centre for Genome Biology (CGB)” that is the result of a is a collaborative effort of four research groups from the University of Bologna and it is involved in genome sequencing and bioinformatics analysis of genomic data.

Main tasks in the proposal

UNIBO is coordinating the WP1 and it is involved in all tasks from 1.1 to 1.4. This WP is aiming to describe local pig breeds based on phenotypic and genomic aspects to develop tools for animal and meat traceability and authentication. As pointed out through the list of previous research projects developed by the department and the list of publications, UNIBO, through the activity of its DISTAL Department, have a previous long experience in these topics as well as the facilities to perform the analyses. In detail DISTAL, within UNIBO, has the skills and technical possibilities to be involved in activities of collecting DNA samples, genotyping, SNP filtering, statistical analyses of SNP data, identification of major genes, resequencing and data analyses, data mining of the Porcine 60K SNP BeadChip data, candidate gene SNP data, mining of the genome sequencing data, evaluation of the appropriate statistical approach, identification of the most informative markers for the different populations and useful for specific cases, identification of the minimum number of SNP for breed allocation, development of a specific genotyping tool, test in specific field conditions the usefulnes of the genotyping tool developed.

For WP2 activities attributed to UNIBO of life cycle analysis and evaluation of impact of management and feeding strategies on animal welfare and environmental issues, the Department of DISTAL, belonging to UNIBO, and in particular the group of Animal Science demonstrated in previous researches on similar aspects its skills and ability in dealing with the meat chain of pigs at all levels.

A contribution is expected by UNIBO in WP3 mainly dedicated to the development of a toolbox. In this specific area the knowledge of DISTAL Department on pig meat parameters and quality gained with a long experience in the field well fit with the requirement of this specific tasks.

Finally, UNIBO will be involved in WP5 – dissemination (publications and participation on meetings, collective trademark development, web and media dissemination).

Profile of the most relevant staff members who will be undertaking the work

Luca Fontanesi, associate professor at UNIBO, is working on the identification and mapping of genes in livestock species and the application of molecular genetics for the improvement and traceability of animal products in pigs from more than twenty years. He has been, or he is, involved in 35 research projects, 5 of each funded by EU. He is chair of the COST Action TD1101 "A Collaborative European Network on Rabbit Genome Biology (RGB-Net)" (2011-2015) - http://www.biocomp.unibo.it/rabbit/ and has more than 120 publication cited in Scopus (June 2014). Leonardo Nanni Costa is full professor at UNIBO and involved in researches in pigs on carcass and meat quality with special attention to the quality parameters of typical PDO productions, as i.e. the Parma ham, and the effect of pre-slaughtering parameters on the final pig product for the market.

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Stefania Dall’Olio, associate professor at UNIBO, is working since 1990 on researches on the variability of gene candidate for carcass traits and meat quality in pigs and on product traceability based on the molecular approach.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Fontanesi L, Schiavo G, Galimberti G, Calò DG, and Russo V. A genomewide association study for average daily gain in Italian Large White pigs. Journal of Animal Science, 2014 Apr; 92(4):1385-94.

2. Schiavo G, Dolezal MA, Scotti E, Bertolini F, Calò DG, Galimberti G, Russo V, and L. Fontanesi. Copy number variants in Italian Large White pigs detected using high density single nucleotide polymorphisms and their association with back fat thickness. Animal Genetics, 2014, accepted for publication.

3. Fontanesi L, Schiavo G, Galimberti G, Calò DG, Scotti E, Martelli PL, Buttazzoni L, Casadio R, Russo V, A genome wide association study for backfat thickness in Italian Large White pigs highlights new regions affecting fat deposition including neuronal genes. BMC Genomics, 2012, 13:583 doi:10.1186/1471-2164- 13-583.

4. Fontanesi L., Bertolini F., Dall'Olio S., Buttazzoni L., Gallo M., Russo V., Analysis of association between the MUC4 g.8227C>G polymorphism and production traits in Italian heavy pigs using a selective genotyping approach, Animal Biotechnology, 2012, 23, pp. 147-155

5. Fontanesi L., Bertolini F., Scotti E., Trevisi P., Buttazzoni L., Dall’Olio S., Davoli R., Bosi P., Russo V., Polymorphisms in an obesity-related gene (PCSK1) are associated with fat deposition and production traits in Italian heavy pigs, Animal, 2012, 6, pp. 1913–1924

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

1. Project Innovagen of the Italian Minister of Agriculture - Application of genomics for improving selection in Italian heavy pigs : pork subunits (2011-2013) and Project Innovagen of the Italian Minister of Agriculture “Traceability of livestock production” (2011-2013); 2. Project AGER - Foundations Savings – “Advanced research in genomics and processing technologies for the Italian heavy pig production chain ( Hepiget )” (2011-2014 ) ; 3. Project Cozac of the Italian Minister of Agriculture "Identification of the characteristics of fresh pork relevant to the transformation in PDO" (2010-2013 ); 4. Project Bov-MAS (FP5 programme of the EU, QLK5-CT-2001-02379) “Quantitative trait loci affecting milk production: mapping and utilization for marker assisted selection in dairy and dual purpose cattle.(BOVMAS)” (2001-2005); 5. Strategic project FAGenomicH (Farm Animal Genomics for Humans) financed by the University of Bologna (2007-2010) (http://fagenomich.biocomp.unibo.it/), coordinated by Luca Fontanesi.

Patents RM2005 A000235 (2005- ) – “Tracer Natural Food”, patent co-invented by Luca Fontanesi (UNIBO), that uses the molecular genetics for the traceability of agro-food products.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Centre for Genome Biology (CGB), University of Bologna, Italy: The Centre for Genome Biology is a collaborative effort at the University of Bologna of four research groups whose activities require genome sequencing and bioinformatics analysis of genomic data. Projects are mainly devoted to modern biological problems where molecular sequencing or resequencing of DNA or RNA can help unravelling the complexity of cells, tissues and organisms. Facilities of the Centre for Genome Biology (CGB) are equipped with advanced

51 TREASURE 634476 molecular genetics instruments and servers for data analysis. The CGB has a PGM Ion Torrent sequencer with all supporting instruments and tools. The Centre for Genome Biology can provide expertises in molecular genetics (including next generation sequencing), genomics, genetics, statistical genomics, bioinformatics and computational biology that may be useful in most biomedical research fields.

The Department of Department of Agricultural and Food Science and Technology has both a wet and dry laboratory with specific expertises. The web lab performs researches in genetics and genomics, i.e. Sanger sequencing, PCR analyses, DNA extraction and amplifications with competencies in extracting and preparing DNA samples to be sent for more sophisticated analyses. The dry lab is involved in bioinformatics and animal breeding analyses.

Partner 14 - STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA)

Description of the organisation

SSICA – Stazione Sperimentale per l’Industria delle Conserve Alimentari is an Institution for applied research established in Parma in 1922, with the specific aim of promoting technical and technological progress in the Italian food industry. In 1999 it was transformed into a public economic institution with legal and administrative modifications. SSICA, since 2011, according to a Government Decree has been affiliated with the Chamber of Commerce of Parma still maintaining its status of national and international research organization. To date SSICA is one of the most important institutions of applied research in the food preservation sector existing in Europe. SSICA is organized in departments specific to preserved food origin (meat, fish and vegetables), safety (microbiological and chemical) and packaging. SSICA is equipped to develop new products, new processing and preservation procedures. Mission. Priority missions of SSICA include support and assistance to enterprises, scientific and technological presidium in application of regulations, to consolidate typical products, special attention to nutritional and safety issues . An important activity of SSICA is dissemination of knowledge in food preserving sector through communication, training and teaching at affiliated trade association, Institution, companies and schools . The quarterly Industria Conserve reports original works by internal and/or external researchers together with economic, scientific, technical and legislative news relating to the food preserving sector. Some technical monographs have also been published, mainly destined to technical personnel of preserved food companies. Furthermore, publications in international scientific journals are a tool for SSICA researchers to disseminate their activity. Research and pilot-scale facilities: Applied researches and pilot-scale experimental tests carried out at SSICA are focused on chemical, instrumental, technological and microbiological properties of intermediate and final outcomes. Analytical research instruments and pilot plants enable adequate industrial experimentation to be performed. Technological departments (e.g. Meat and Vegetable departments) have a broad experience in all steps of production chain from raw matter suitability to processing to final product packaging.

Main tasks in the proposal

SSICA has a large experience in the production techniques with reference to the typicality of the products and to the mapping of manufacturing parameters. The department of meat products investigated the characteristics of processed meat products and the impact of the production process back on the territory. In TREASURE project, SSICA will take part in dissemination and demonstration activities, in particular in the transfer of knowledge (WP5), through the organization of a dry cured meat products school. The aims of the latter are: a) to transfer to small and medium enterprises the information oriented to the valorisation of typical meat product of Med area, b) to promote the exchange of experiences and networking among producers, transformers and relevant stakeholders. The achievement of objectives will be assessed with the number of breeders, farmers and manufacturing structures involved. The operators concerned can acquire knowledge, awareness and new skills of traditional and innovative process.

Profile of the most relevant staff members who will be undertaking the work

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Virgili Roberta took a degree in Chemistry at the University of Parma. In 1987 she became a researcher of the staff of the Meat Department of SSICA. She is author of papers published on national and international journals and speaker in scientific congresses and workshops. Her field of investigation is the interaction between meat quality, processing and properties of the final product, including quality and safety. She is involved in national and 7th Framework Programme EU projects dealing with non-invasive assessment of meat quality, salt reduction and new formulations of meat products. In the period 2004-2014 she is contract Professor of “Technology of Meat Products” at the University of Parma and supervisor of several degree theses.

Diaferia Carlo degree in Chemistry at the University of Naples. After a master in food technology, he became researcher at SSICA. His studies concerned mass and heat transfer in dry cured meat products. Recently he participated at international and national programs regarding the rural development and traditional products . Various papers on these topics are published on national and international journal .

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content 1. P. Fantazzini, M. Gombia, P. Schembri, N. Simoncini, R. Virgili. Use of Magnetic Resonance Imaging for monitoring Parma dry-cured ham processing. Meat Science, 82, 219-227 (2009). 2. R. Benedini, G. Parolari, T. Toscani, R. Virgili. Sensory and texture properties of Italian typical dry- cured hams as related to maturation time and salt content. Meat Science, 90 (2), 431-437. (2012). 3. N. Simoncini, R. Virgili, T. Toscani, G. Spadola, P. Battilani. Autochthonous yeasts as potential biocontrol agents in dry-cured meat products. Food Control, 2014, DOI. 10.1016/j.foodcontrol.2014.04.030. 4. MED Program 2007-2013 : QUBIC Project Final Report “ The sustainable use of biodiversity in MED area : the contribution of the QUBIC Project” 5. S. Porcu, R. Mazzette, C. Diaferia, G. Riu, C. Manca, M. Addis. Qualitative, chemical and nutritional traits of dry-cured ham of sarda breed pigs : effect of rearing system. 8th Int. Symposium on the Mediterranean Pig, October 2013, Ljubljana Slovenia”

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal - MED Program 2007 – 2013 Project “Animal Breeding : Quality, Biodiversity, Innovation, Competitiveness (QUBIC)” G.A. MED08-395 - FP7 SME 2013 Project “Optimization of the salting process for the production of healthier and higher quality dry cured meat products” (PROCURED) G.A. n.605758 - FP7 SME 2013 Project “Phytochemicals to reduce nitrite in meat products” PHYTOME G.A. n. 315683 - AGER Project “Advanced research in genomics and processing technology for the Italian heavy pig production chain “ G.A. n. 2670/2011 - FP7 SME 2011 “Helping EU processors become competitive using automated and non-contact pigmeat piece quality classification” (Q-Meat) G.A. n. 286487

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

The activity of Meat Department is focused on meat products obtained by drying or fermenting (dry-cured meats), heat treatment (cooked meats), or active packaging (sliced meats). The research, developed in collaboration with the industry, associations of producers, consortia, universities and research institutes, aims to provide support to enterprises for the improvement of standards of quality and safety as well as a scientific basis for the entire meat chain, including the suitability of the raw material for processing, monitoring of processing, study of pre-portioned and packaged end products. An important activity is the dissemination of the results achieved. The main traditional activities aiming at improving the technology and the quality of Italian cold cuts go along with topics such as safety, nutrition, novel products development, packaging, shelf-life and sustainability environmental. Two pilot plants, for dried and cooked meat products respectively, have been operating at SSICA since late ’80s. Studies performed in recent years in the field of meat products paid special attention to nutritional value and safety lowering sodium and nitrites, paying attention to mycotoxin contamination and biogenic amines generation, and including bioactive molecules in the formulation (polyphenols, natural pigments and plant extracts) to prevent oxidation and to achieve chemoprotection against radicals. The department has cells for salting, resting, drying, ageing and smoking; cells are controlled by the PROGES software. The pilot plant for cooked products includes several tumblers operating under vacuum, an injecting

53 TREASURE 634476 machine, mincing equipments, a moulder for whole products, dry and steam ovens. A Software to calculate heat treatment letality is available. An updated analytical instrumentation (Instron, HPLCs, GC-MS, NIR, Orbitrap, ICP-MS, Photochem, DSC, 2D-Electrophoresis, Capillary Electrophoresis, qPCR) is employed for an extensive characterization of meat products. A trained sensory panel is available.

Partner 15 - LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS)

Description of the organisation

Institute of Animal Science of Lithuanian University of Health Sciences was founded in 1952. The research is conducted by five departments and two laboratories. The Institute has a 92 persons staff, 29 of which are scientific experts including 23 doctors and 6 postgraduated students. The main research activities of the Institute are the studies of animal breeding and genetics; biology and management of the local populations of animal genetic resources, production quality and biology of reproduction; studies on animal nutrition and on animal welfare and environmental issues related with animal production. The Institute participates in the activities of the centre Nemunas which integrates science, studies and business. The Institute has prevented total disappearing of unique Lithuanian indigenous wattle pig breed and maintains small populations of Lithuanian indigenous wattle, Lithuanian White (old genotype).

Main tasks in the proposal

Phenotypic description of Lithuanian local pig breeds (Lithuanian indigenous wattle and old type Lithuanian White). Collection of the blood samples and DNA isolation for local breed genetic characterization and comparison with other European local breeds. Evaluation of productivity of Lithuanian local pig breeds under varying growth intensity, including effect of restrictive diet. Evaluation and characterization of the carcass and meat, and lipid quality from of Lithuanian pig genetic resources. Estimation of the effect of local pig gender on carcass and meat quality. Evaluation of consumer acceptability of pork and traditional Lithuanian national pork products from local Lithuanian pig breeds. In WP5, LUHS will be involved in the development of publications and participation on meetings, and in demonstration activities.

Profile of the most relevant staff members who will be undertaking the work

Violeta Razmaitė PhD Main research interests is in the area of pig production, meat quality, wider use of local animal genetic resources and their adaptation to different production niches, food diversification using domestic and wild animal genetic resources, meat and animal fats components having influence on consumer health, consumption attitudes. She was involved in the national project “Genofondas” and coordinated the research themes of farm animal (1998-2002), and in the international project named “Evaluation of the pig breeding structure in Lithuania” approved by Ministry of Foreign Affairs of Lithuania and SIDA (Swedish International Development Cooperation Agency) – 2004-2005. She is a head of the project “Quality of meat of farm and wild animals from different rearing systems in relation to healthy nutrition“(2012-2015). Ruta Sveistiene PhD Main research interests is in the area of the genetic variation and wider use of local animal genetic resources. She is the head of Lithuanian Center for Farm Animal Genetic Resources Conservation. She was involved in EU FP6 project 40 position for Early-Career Scientists in meat quality and safety are available within an EU Specific Support Action (SSA) coordinated by EAAP within partners - YOUNG TRAIN (2005-2008). Virginija Jatkauskienė, is a researcher in the department of animal breeding and genetics. Scientific fields: genetic evaluation of animals Gintautas Juozas Svirmickas PhD Main research interests is in the area of the analysis of animal production composition and quality, the analysis of plants and animal feed, development and application of near infrared spectroscopy methods for analysis of animal and vegetable samples. Ausra Bajorinaitė Main research interests is in the area of the analysis of pork quality and consumers attitudes

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Saulius Bliznikas PhD Research activities include a quantitative and qualitative analysis of meat quality and lipid oxidation parameters - amino acids, cholesterol, malondialdehyde - by using of liquid chromatography methods Arturas Siukscius PhD The main research activities is in the area of investigation of the cryopreservation processes on boar semen quality, development of freezing methods for boar semen and methodical guidelines for pig insemination in Lithuania, and implementation of cryopreserved boar semen, establishment of the semen and embryo bank for endangered indigenous farm animals. Remigijus Juska PhD Main research interests is in the area of farm animal housing technologies, ecology of animal husbandry, impact of pig farming on environment, use of biologically active materials in the pig nutrition for improvement of pork quality. Raimondas Leikus PhD Main research interests is in the area of pig feeding

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Razmaitė V., Kerzienė S. and Šiukščius A. Pork fat composition of male hybrids from Lithuanian indigenous wattle pigs and wild boar intercross. Food Science and Technology International, 2008, 14(3). P. 251-257. 2. Razmaitė V., Švirmickas G. J. Comparison of fatty acid composition in different pig tissues. Veterinarija ir Zootechnika. 2012. T. 58 (80). P. 77-82. 3. Razmaitė V., Jatkauskienė V., Juozaitienė V. Prolificacy of old genotype Lithuanian White sows in small closed population. Acta Veterinaria (Beogr). 2012. Vol. 62 (4). P. 355-363. 4. Razmaitė V. and Švirmickas G. J Fatty acid composition in hybrid pigs as based on local fatty lithuanian breed and wild boar. Food Science and Technology International. 2012. Vol. 18(6) P. 515–522. 5. Juska R., Juskiene V., Leikus R. The influence of a free-range housing system on pig growth, carcass composition and meat quality. Journal of Applied Animal Research. 2013. Vol. 41(1). P. 39-47.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

 The National research programme “Genofondas” (1998-2002)  The international project “Evaluation of the pig breeding structure in Lithuania” approved by Ministry of Foreign Affairs of Lithuania and SIDA (Swedish International Development Cooperation Agency) (2004-2005)  FP6: EU project 40 position for Early-Career Scientists in meat quality and safety are available within an EU Specific Support Action (SSA) coordinated by EAAP within partners - YOUNG TRAIN (2005- 2008)  The project “Quality of meat of farm and wild animals from different rearing systems in relation to healthy nutrition“ (2012-2015) of the National programme “Healthy and safe food”  EU Cost FAIM 1102 “Optimising and standardising non-destructive imaging and spectroscopic methods to improve the determination of body composition and meat quality in farm animals“(2012- 2015).

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

In the laboratories of Institute of Animal Science of LUHS a unique scientific equipment is available: a gas liquid chromatograph (GC – 2010 Shimadzu), “GC solution” software for Shimadzu gas chromatograph workstations and the HPLC system Shimadzu 10 AVP (Shimadzu Corporation, Japan), Texture Analyser TA 1 (Measurement and Calibration Technologies Ametek Comp., Lloyd instruments, Largo, FL. USA), the chromameter CR-410 (Konica Minolta, Japan), Calimatic, Kjeltec system 1002 apparatus (Sweden), Soxhlet apparatus (Gerhardt, Germany), atomic absorption spectrophotometer Perkin-Elmer 603 (USA), ELISA reader and equipment for DNA purification: Microcentrifuge MSE Microcentaur, Thermomixer MKR 13, Shaker- vortex VELP scientifica, Spectrofotometer "Spectronic Biomate 3 Vv-Vis, Laminar box Hyperchan 36 and Freezer Platinum 500V for storage of DNA samples (Italy).

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Partner 16 - INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC)

Description of the organisation

The Polytechnic Institute of Viana do Castelo (IPVC) was founded in 1980. It is a regional institution, whose aims are: to supply qualified human, cultural, scientific, technical and professional training, to carry out necessary research to accomplish its mission and to cooperate with the regional community of Alto-Minho, particularly with its productive and entrepreneurial sectors. The IPVC is made up of six Schools offering 21 specialization courses, 40 undergraduate and 34 postgraduate degree courses. The undergraduate students very often are in collaboration with other national and international polytechnics and universities. IPVC has engaged in strong endeavours to establish partnerships with other European Institutions, being now nationally acknowledged as a leading research and development institution in tourism, ICT, technology transfer and innovation areas. EU funded projects have been carried out under several Programmes, including REDF-art. 10, Fifth Framework, FP7, Interreg II C Atlantic Area, POCTEP, SUDOE, Tempus, Socrates, Leonardo da Vinci, Erasmus Mundus and ALFA III. Main tasks in the proposal: Please explain how the profile matches the tasks in the proposal The partner IPVC already has the needed facilities for the Bísaro pig studies, whether land or laboratories. It explores a 17 ha farm producing varied agricultural products, and 17 more ha of forests. It also keeps herd of Bísaro pigs for research purposes. Within this project we intent to increase herd size and facilities. IPVC has research laboratories well equipped that will serve this project. It has chemical labs with analytical research equipment’s like GCMS, several GC, HPLC, sensorial labs and microbiological labs.

The main tasks proposed by IPVC partner are within WP2-Management and performance of local pig breeds in their production systems and WP3-Traditional and new high quality products with regional identity. To achieve these goals, other tasks within WP1, WP4 and WP5 will be developed as follows: WP1 Description and evaluation of local pig genetic resources (Luis Pinho, Eliana Barbosa, ANCSUB) Task 1.1 Up-grade the phenotypic characterization of local pig breeds. Task 1.2 until Task 1.7. Collection and identification of samples to be studied by UNIBO (Italy) partner (?) WP2 Management and performance of local pig breeds in their production systems (José Pedro, Joaquim Cerqueira, João Silva, Eliana Barbosa, Luis Pinho) Task 2.1 Comparative analysis through survey, review and/or meta-analysis of reproductive and fattening traits based on data collected in Bísaro farms. To support WP3 and WP4, meat quality, indicators of animal welfare, environmental impact and economic performance will be assessed. Task 2.4 Experiments to evaluate innovative feeding strategies or management practices involving animal welfare and health, and their effects on production traits and product quality. Development of an experimental swine production system, Hoop Barn model, in IPVC. This experimental system will be developed to test the effect of inclusion of special local feeding resources, a) potatoes, b) germinated seeds, c) vegetables by-products from local markets. WP3 Traditional and new high quality products with regional identity (Preciosa Pires, José Pedro, J. Silva) Task 3.1 A toolbox will be developed for the evaluation of sensory, nutritional and technological meat quality traits (besides evaluation of sensory and microbiological evaluation, IPVC proposes the study of fatty acid profile, minerals content with emphasis in sodium and potassium, lactic acid and other small organic acids, in meat and meat products). Some of these parameters could be related to determine genuineness of Bísaro pig products. Task 3.2 Quality evaluation toolbox developed in Task 3.1 will be used to determine i) sensory ii) nutritional iii) technological qualities iv) genuineness of high quality pork products from local pig breeds and production systems. Quality of pork products will be characterized according to local breed, animal feeding/management practices (link with WP2) and processing techniques. Task 3.4 Database on systematic determinations of carcass and meat quality traits will be set up and implemented, together with experts and actors of breeding organizations, as part of a wider activity aiming at implementing strategies of breeding programs in the concerned pig breeds. WP4 Consumer preferences and market research for sustainable pork chains (José C. Santos, J. Santos Silva) Task 4.1 Cost-Benefit Analysis at farm and product level, considering private costs and benefits; identification of management practices that can optimize economic performance. Task 4.2 Cost-Benefit Analysis for the society, considering public costs and benefits. Socioeconomic studies aimed at characterizing regional Bísaro pig producers. WP5 Dissemination and demonstration activities (ANCSUB, IPVC)

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Task 5.1 research results will be presented to the scientific community through scientific publications (journals, books) and contributions in international symposia. Task 5.4 demonstration activities will be targeted at transferring acquired experience/expertise of well- established and successful pork production sectors to partners in other regions where pork production sectors based on local breeds are still at a startup stage. Organization of demonstration activities with the support of the associated third party, ANCSUB.

Profile of the most relevant staff members who will be undertaking the work

José Pedro Araújo: PhD in 2006 by Santiago de Compostela Univ (USC), Spain. Dep. Anal. Chem., Nutri. and Food Sci. Spain. Research on meat quality of local breeds, their productive characterization, organic farming and welfare. Preciosa Pires: PhD in 1995 by University of Liverpool, United Kingdom. Dep. of Chemistry. Research on food chemistry including analytical chemistry – study of fat composition (fatty acid analysis). Joaquim Orlando Lima Cerqueira: PhD (2013) in veterinary sciences. Oporto University (Instit. Ciências Biomédicas Abel Salazar). Research on animal production and welfare and characterization of local breeds. Eliana Pamela Barbosa PhD (2009). Leon Univ (Faculty of Veterinária). Research in characterization and genetic improvement of portuguese native breeds, as well as the promotion and dissemination of their traditional products. Luis André Pinho: PhD (2003). Veterinary sciences. Oporto Univ (Instit. Ciências Biomédicas Abel Salazar). Research in microbiology and epidemiology of mastitis in cattle and consultancy in the field of medicine production. José Carlos Santos: PhD (2011). Agricultural Economics. Polytechnic Univ of Madrid (Faculty of Agronomy). Research in Rural Development and Sustainability, project management and appraisal, and environmental economics.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Cerqueira, J.O., Feás, X., Iglesias, A., Pacheco, L.F., Araújo, J.P. (2011). Bordaleira de Entre Douro e Minho” sheep. Divergence of the breed. Animal Production Science, 2011, 51, 635–641 2. Feas, X., Pires, J., Estevinho, M.L., Iglesias, A., Araujo, J.P., 2010. Palynological and physicochemical data characterisation of honeys produced in the Entre-Douro e Minho region of Portugal. International Journal of Food Science and Technology, 45, 1255–1262. 3. Principal component analysis of proteolytic profiles as markers of authenticity of PDO cheeses, J. S. Guerreiro, M. Barros, P. Fernandes, P. Pires, Ronald Bardsley, Food Chemistry 136, 1526–1532, 2013. 4. Chemical, Microbiological and Sensory Characterization of a Traditional Presunto (Smoked Ham) from Portuguese Autochthonous Bísaro Pigs – a Comparison with Industrial Presunto, Carina Cruz, E. Fernandes, M. Vilarinho, M. Barros, Preciosa Pires, R. Ferreira, O. Morais, Luís Correia, C. Ramos, Joana Santos, Paulo Fernandes, Peter Ho e Manuela Vaz Velho, Electron. J. Environm. Agric. Food Chem. 2(4), 2003, ISSN 1579-4377.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

Integral assessment of risk-based intervention strategies to improve the microbial safety of Portuguese traditional sausages. Projeto FCT - PTDC/AGR-TEC/3107/2012. Water Quality and Management in the Agro-Food Industries, 2011-13. Leonardo Da Vinci, 2011-1-GR1- LEO04-06776 6. Chemical, Microbiological and Sensory Characterization of a Traditional Presunto (Smoked Ham) from Portuguese Autochthonous Bísaro Pigs – a Comparison with Industrial Presunto- CEDA – Centro Europeu para a Dieta Atlântica”, 2001-2003. CCDRN Programa Operacional da Região do Norte/Operação Norte/Eixo Prioritário 2/Acções Integradas de Base Territorial/Medida 2.2 (Minho Lima) - 2.2-FDR-00006. Organisation of Piglet Bísara Breed Gastronomic Event, IPVC, 7/12/2013.

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Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

IPVC has available a zootechnical housing with various local breeds and veterinary support room laboratories (life sciences and biotechnology, animal and watering), Agricultural and forestry areas correspond to 34 ha: agricultural Greenhouses; Orchard and vineyard with drop-a-drop irrigation, arable crops and forest (17 ha). The IPVC has technical/research facilities to support teaching, research and analytical services to the community, since 1991. The Institution has as a Certified Laboratory in Microbiology (UMA-Unidade de Microbiologia Aplicada) since 2002. This is certified by NP EN ISO/IEC 17025:2005, with certified number L0359 by IPAC since 2005. Also, IPVC received financial support 1.295.558,00 euros funding, from ON.2, Programa Operacional Regional do Norte -01-0162-FEDER-000027, to equip with scientific resources their laboratories, with the aim of supporting the development of the region. Within this funding, IPVC has recent analytical resources like: GCMS, several GC, HPLC (Detector UV/Vis Diode-Array; Gama: 190 a 900 nm; Díodos: 1024), sensorial lab, between others for microbiology lab.

Partner 17 - UNIVERSIDADE DE EVORA (UEVORA)

Description of the organisation

The University of Évora is organized in 4 Schools: Arts, Sciences and Technology, Social Sciences and Nursing and offers 41 undergraduate and 120 postgraduate degrees. Research and Development (R&D) covers several scientific areas through a network of 14 Research Units, all of them submitted to international evaluation, under the coordination of the Institute for Research and Advanced Studies. Furthermore, the University of Évora established three Chairs in areas of excellence: Biodiversity, Renewable Energies and Heritage, which are sponsored by private capital holders. Over the last years, the University has fostered a close link with the community. Such interaction has been possible through the creation of working networks and dissemination of knowledge such as the participation in the Science and Technology Park as well as through the establishment of protocols and contracts for the supply of services in partnerships with Companies, Universities, Associations and Institutes. The main R&D areas are: Agronomy and Biodiversity; Geophysics, Environment and Landscaping; Materials and Surface Science; Economics and Business Studies; Computer Sciences and Software Interoperability; Social and Political Sciences, History, History of Art, Science and Cultures; Applied Mathematics; Education; Linguistics and Literature; Elderly Healthcare. The 150 running R&D projects are developed through national and international partnerships, funded by the FP7, FCT as well as by private sponsorship.

Main tasks in the proposal

Since its reopen the University of Évora in 1979 has a tradition in teaching and research in Agronomy and Animal Science. The opening of Veterinary Medicine formation in the late 90’s reinforced that large area of knowledge. Besides the education, knowledge transfer, and services to society, the University of Évora, has knowledge production throughout scientific research, being this last mission carry out through the University Research Institutes. All of the researchers participating in the tasks are involved in Animal Science, Agronomy and Veterinary Medicine teaching and are members of the Institute of Mediterranean Agricultural and Environmental Science (ICAAM) which is a Research and Development (R&D) Unit created in 1991. ICAAM is located on “Campus da Mitra”, an experimental farm covering more than 285 ha in Alentejo (southern Portugal), 12 km from the city of Évora. ICAAM’s main goal is to promote excellence in research on Mediterranean agro-ecosystems, following a multidisciplinary approach (integrating researchers from several scientific areas: Agricultural Engineering, Animal Science, Food Technology and Innovation Biology, Crop Science, Physics, Chemistry, Ecology, Economics, Landscape and Territory, Soil Science and Veterinary Medicine) and a strong cooperation with national and international research groups and local producers and professional organizations. As a result, ICAAM aims to contribute to the knowledge, innovation, development, sustainability and competitiveness of agro-silvo-pastoral Mediterranean systems. All the research staff has experience in Research projects at European and National levels (some listed below). The University and ICAAM have besides the above mentioned research farm, two farms located in south Alentejo, and a group of facilities and laboratories equipped for animal and veterinary science research (listed below). Our research

58 TREASURE 634476 team has access to several private enterprises in all the pig production chain, namely pig farms, slaughterhouses, transformation plants and commercialization enterprises. All our most relevant staff formation, all our scientific experience in the related fields and the connection between UEVORA and ICAAM with national and international research groups and local producers and professional organizations clearly matches with the proposed tasks by the UEVORA in all WP’s. Specifically, for our experimental trails, our team has long experience both in pig zootechnical trails (performance, feeding test, etc), that will be performed in WP2, and in pig carcass, meat and products analyses, that will be done in WP3, as can be proven by the international and national projects where we’ve been involved and also by our scientific publications. In WP5, UEVORA will be involved in the production of publications and participation on meetings and in demonstration activities. Moreover, UEVORA will organise the 8th International symposium on Mediterranean pig.

Profile of the most relevant staff members who will be undertaking the work

José Luís Tirapicos Nunes. PhD in Agricultural Sciences, University of Évora (1993). Main scientific areas of research: Animal reproduction, Swine science. Coordinator of international and national research projects. Miguel Nuno Geraldo Viegas dos Santos Elias. PhD in Food Engineering, University of Évora (2005). Main scientific areas of research: Food technology/food biotechnology. Founder and associate of an entrepise producer of AL pig breed traditional sausages. José Manuel Mota Ruivo Martins. PhD in Agricultural Sciences, University of Évora (2005). Main scientific areas of research: Nutrition and lipid and cholesterol metabolisms. Rui Miguel Carracha Charneca Charneca. PhD in Veterinary Sciences, University of Évora (2010). Main scientific areas of research: Swine reproduction, maternal ability of sows, Newborn piglet physiology and behaviour. Amadeu António Gomes Borges de Freitas. PhD in Agricultural Sciences, University of Évora (1999). Main scientific area of research: Animal nutrition and feeding, production systems of monogastrics. José Alberto Macedo Neves. PhD in Agricultural Sciences, University of Évora (1999). Main scientific areas of research: Meat Science and Technology.

All most relevant staff members have several publications in peer-review international and national journals and participations in national and/or international research projects, related to the proposal.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Elias, M., Potes, M.E., Roseiro, L.C., Santos, C., Gomes, A., Agulheiro-Santos,A.C.(2014). The Effect of Starter Cultures on the Portuguese Traditional Sausage “Paio do Alentejo” in Terms of Its Sensory and Textural Characteristics and Polycyclic Aromatic Hydrocarbons Profile. Journal of Food Research 3 (3): 45-56. 2. Martins, J.M.; J.A. Neves; A. Freitas; J.L. Tirapicos (2012). Effect of long term betaine supplementation on chemical and physical characteristics of three muscles from the Alentejano pig. Journal of the Science of Food and Agriculture, 92 (10), 2122-2127. 3. Charneca R. , J.L.T. Nunes and J. Le Dividich (2010). Body composition and blood parameters of newborn piglets from Alentejano and conventional (Large-White x Landrace) genotype. Spanish Journal of Agricultural Research 8 (2), 317-325. 4. Elias, M., Carrascosa, A.V. (2010). Characterization of the Paio do Alentejo, a traditional Portuguese Iberian sausage, in respect to its safety. Food Control (21): 97-102 5. Martins, J.M.; J.A. Neves; A. Freitas; J.L. Tirapicos (2010). Betaine supplementation affects the cholesterol but not the lipid profile of pigs. European Journal of Lipid Science and Technology, 112 (3), 295-303.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

 Project CAMAR CT 90 0013 – “Porcs Méditerranéens en Systèmes Sylvo-Pastoraux: Optimisation de la Production de Porcs Destinés à la Transformation en Produits Secs de Haute Qualité” (1991 - 1995).  Project Agro 226 "Optimization of the production of Alentejano pigs for the production of fresh meat for consumption".(2002 – 2005). 59 TREASURE 634476

 Project POCTI/CVT/60411 "Effect of pasture feeding of Alentejano pigs produced in “montanheira". (2005 – 2008).  Project PTDC/AGR-ALI/119075/2010. Portuguese traditional meat products: strategies to improve safety and quality. (2012-2014).  Projecto MITTIC - POCTEP: “Precision feeding of Iberic pigs” (2014-2015)

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

The University of Évora has 3 farms, one of them located where the laboratories and other support facilities are also placed (Mitra Campus). We have swine facilities both for indoor and outdoor pig production. We have well equipped laboratories on the areas of: animal nutrition and metabolism, biochemistry, molecular biology, microbiology, reproduction and lactation, regional products technology, food texture.

Partner 18 - Institut za stočarstvo, Beograd-Zemun (IAH)

Description of the organisation

Institute for animal husbandry is scientific institution specialized in fundamental, applied and development research in the field of biotechnical sciences. It is organized in 6 research departments: - Genetics and improvement of livestock; - Physiology and nutrition of livestock; - Breeding and reproduction of livestock; - Meat technology; - Livestock selection and main recording; - Livestock feed and ecology. Institute has accredited laboratory (chemical and microbiological), slaughterhouse and meat processing plant, experimental field of 300 ha of land, 5 experimental farms and feed mill. We are highly experienced in: - Research, development and application of modern methods for evaluation of breeding value and crossing of livestock; - Development and application of modern principles and livestock selection methods; - Study and determination of livestock production traits; - Study of the impact of nutrition on health condition of animals and fattening characteristics (studies are carried out on 5 experimental farms of the Institute: pig farm, cattle farm, sheep and goat farm, poultry farm and experimental feed mill); - Study of new energy and protein sources, as well as specific forms of micro elements in order to obtain final product (meat, milk, eggs) with traits of functional food; - Collecting of scientific and technical information relating to quality, especially changes on meat as consequence of nutrition, genetic characteristics and other intravital and premortal factors; - Study of the slaughter properties of slaughtered animals and processing of obtained meat (manufacturing of meat products in the Experimental slaughterhouse of the Institute); - Study of the quality of animal feed, meat and meat products in chemical and microbiological laboratory of the Institute; - Organization of various questionnaires (consumer opinion) through retail facilities of the Institute and organization of seminars.

Main tasks in the proposal

WP1. Phenotypic description of Serbian autochthonous pig breeds (Mangulica and Moravka) will be done at experimental farm of the Institute for Animal Husbandry. The sampling of blood or hair root for genetic analyses will be done at experimental pig farm of Institute and other relevant farms in Serbia.

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WP2. Breeding of autochthonous breeds of pigs in the farm of Institute and use of locally available feeding resources and collecting data (reproductive and fattening performance, meat quality, indicators of animal welfare, environmental impact and economic performance) for further analyses. Animals which are not part of the nucleus or are not selected for breeding in different housing systems (extensive and semi-intensive), will be included in the programs of commercial production in order to obtain the raw material for the production of traditional products that have importance in rural development. The share of muscle, fat and bone in the carcass of autochthonous breeds of pigs shall be determined using partial or total dissection. WP3. Samples of muscle and fat tissue would be taken for chemical analysis (after slaughtering of pigs in experimental slaughterhouse of the Institute), particularly for the determination of the individual contents of saturated, poly-and monounsaturated fatty acids, cholesterol content, the content of certain heavy metals. The analyses will be done at laboratory of the Institute (Gas chromatography, Atomic absorption spectroscopy). The study would include carcass quality and meat properties of Mangulica and Moravka pigs grown at different sites (lowland and highland) and different castrated animals (immune-castrated and surgically castrated). The samples of muscles and various samples of meat products (high quality pork products, dry fermented sausages - Kulen and Sremska sausage). Sausage manufacture will be done at meat processing plant of the Institute. WP4. Development of Cost-Benefit Analysis of rearing of breeding animals of indigenous breeds (Mangulica and Moravka) in the nucleus (main) herd after performing molecular genetic analysis and confirmation of breed. Cost-benefit analysis of the complete process of production of the finished product (Kulen and Sremska sausages). WP5. Transfer/exchange of knowledge – at International Congress, a meeting organized by the IAH in October 2017 and 2019 and publishing of peer-review papers in international journals and national journals. Furthermore, IAH has a close link to the Serbian pig breeders and meat industry and will ensure direct dissemination of project objectives and results. In addition, IAH will organize a presentation of TREASURE at fair »Fair of ethnic food and drinks« in Belgrade.

Profile of the most relevant staff members who will be undertaking the work Čedomir Radović, PhD EDUCATION 1994 – 2000: University of Belgrade, Faculty of agriculture, Department of animal science. 2000 – 2007: Post-graduate studies, University of Belgrade, Faculty of agriculture, Department of animal science. Title of MSci thesis: Evaluation of the breeding value of boars based on traits of their progeny. 2013: PhD, University of Belgrade, Faculty of agriculture, Department of animal science. Title of thesis: Phenotypic and genetic variability of quality traits of pig carcass sides and meat. 2006 Certificate of Achievement GAP- Good Agricultural Practice 2009 Certificate – Training in Animal Welfare EXPERIENCES Position in the organization: deputy director WORK EXPERIENCE Researcher; present status “research Assistant” 2001 - 2002: Research trainee 2002 - 2013: Research assistant 2014: Research associates 2007-2010: Lecturer at the Training and Information Center on Good Agricultural Practice Field research: Genetics and improvement of pig

Nikola Stanišić, PhD EDUCATION 2001 – 2007: MSciat University of Belgrade, Faculty of Agriculture (Serbia), Department of Animal Food Technology. 2012: Study-research visitin theUSA(Iowa State). This study tour was part of an project between USDA and the Ministry of Agriculture, Trade, Forestry and Water Management of the Republic of Serbia: Grades and standards of commercial meat cutting in the meat processing industry and roles of USDA/AMS. 2007 - 2013PhD at the University of Belgrade, Faculty of Agriculture, Department of Animal Food Technology. Title of thesis: THE EFFECTS OF USING CONJUGATED LINOLEIC ACID IN THE DIET OF PIGS ON FATTENING PERFORMANCE, CARCASS COMPOSITION AND QUALITY OF MUSCLE AND FAT TISSUE EXPERIENCES WORK EXPERIENCE

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Researcher; present status “research Assistant” From 2011:Researcher assistant 2007 - 2011: Research trainee Field research: Meat science and technology

Miloš Lukić EDUCATION 1995: Bachelor of Agricultural Sciences (Agricultural Faculty in Belgrade) 2002: Master of Science (Faculty of Veterinary Medicine in Belgrade) 2009: Ph.D. (Agricultural Faculty in Belgrade) EXPERIENCES Position in the organization: director WORK EXPERIENCE From 1996: Scientific researcher at the Institute for Animal Husbandry, Belgrade-Zemun 2008 - 2010: Deputy director of the Institute for Animal Husbandry, Belgrade – Zemun From 2010: Director of the Institute for Animal Husbandry, Belgrade – Zemun Field research: Production, nutrition and physiology of domestic animals (non ruminants), Good Agricultural Practice (GAP)

Marija Gogić EDUCATION 2002 – 2010: Faculty of Agriculture Belgrade (Serbia), Department for Zoo Techniques 2010 - 2013: Enrolled in Ph.D. studies at the Department for Zoo Techiques, Department forBreeding and Reproduction of domestic animals - at University of Belgrade – Faculty of Agriculture. Title of thesis: Not registered yet EXPERIENCES WORK EXPERIENCE Researcher; present status “research Assistant” 2011-2012: Scholarship from the Ministry of Science 2012-2013: Research Trainee From 2013: Research Assistant Field research: Breeding and reproduction of pigs

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Stajić S., Perunović M., Stanišić N., Žujović M., Živković D. (2013): Sucuk (turkish-style dry-fermented sausage) quality as an influence of recipe formulation and inoculation of starter cultures. Journal of Food Processing and Preservation, 37(5), 870-880. 2. Radović Č. Petrović M., Parunović N., Mijatović M., Radojković D., Stanišić N. 2010. Elaborat: Institute sausage made from pig meat from fatty and meaty breeds. Biotechnology in Animal Husbandry, 26, Spec. Issue. 95-105. 3. Parunović N., Petrović M., Matekalo-Sverak V., Radojković D., Radović Č., Stanišić N., Vranić D.(2013): Fatty caid profile, cholesterol content, atherogenic (IA) and thrombogenic (IT) health lipid indices of different fermented dry sausages. 59thm International Congress of Meat Science and Technology, 18- 23rdAugust 2013, Izmir, Turkey. Proceedings. 4. Stanišić N., Lilić S., Petrović M., Živković D., Radović Č., Petričević M., Gogić M. (2012): Proximate composition and sensory characteristics of Sremska sausage produced in a traditional smoking house. 6th Central European Congress on Food, CEFood2012, Novi Sad, Serbia, 23-26. May, Proceedings, 1319- 1324. 5. Živković D., Radulović Z., Aleksić S., Perunović M., Stajić S., Stanišić N., Radović Č. (2012): Chemical, sensory and microbiological characteristics of Sremska sausage (traditional dry-fermented Serbian sausage) as affected by pig breed. African Journal of Biotechnology, 11, 16, 3858-3867.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

1. Collection, selection and identification of animals of Moravka and Resavka breeds and work on their preservation (No. 401-00-2100/2004-09; project coordinator Prof. Dr.MilicaPetrović,FA);

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2. Comparative investigation of the traits of carcass quality and meat from autochthonous pig breeds (No. 401-00-4227/2005-09; project coordinator Prof.Milica Petrović,FA); 3. Application of molecular-genetic methods in the determination of the genetic variability within and between endangered pig breeds - Mangulica and Moravka (ААР007, STAR project coordinator Prof.MilicaPetrovic,FA); 4. Application of various breeding-selection and biotechnological methods in improvement of pigs (ЕBP: ТР31081; project coordinator Prof.Milica Petrović,FA). 5. Consolidation of data base on existing herds of autochthonous cattle, buffalo, horse, sheep, goat and pig breeds (No. 320-07-5471-1/2/2007-03; project coordinator Dr Čedomir Radović,IAH)

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Institute has accredited laboratory (chemical and microbiological, GC-FID, AAS, ELISA spectrophotometer), slaughterhouse and meat processing plant, experimental field of 300 ha of land, experimental pig farm and feed mill.

Partner 19 - FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG)

Description of the organisation

The University of Belgrade-Faculty of Agriculture has long tradition, it is a teaching and research institution whose activities cover all aspects of agricultural production and food technology: crop science, fruit science and viticulture, animal science, soil management, phytomendicine, agricultural engineering, food technology and agricultural economics. The Faculty is organized in 31 departments and eight Institutes. The Faculty has a Central Laboratory, an Office for Student affairs, Central Library, as well as a Technical and Administrative Services. From 1941/42 the FoA has an experimental school estate “Radmilovac”, of about 100 ha, a legacy of the family Vukicevic. Faculty of Agriculture organizes a number of scientific and professional meetings on a regular basis and with national and international participation (Animal Sciences, Aquaculture, Bee and honey production, Crop Sciences, Phytomendicine, Agricultural Mechanization, Food technology, Milk production, Agroeconomy), as also publishing scientific results in national and international printed and electronic publications, as well as the “Journal of Agricultural Sciences”. The primary mission of the Faculty of Agriculture is in the development of study programs in accordance with the Bologna process, its continuous modernization and harmonization with modern world trends and the state of science in engineering and biotechnical sciences and technical technological scientific field. The Faculty has a large number of laboratories that provide educational, and scientific research and professional activity: Laboratory of Electron Microscopy, Laboratory for land, Laboratory analysis of fruit, grapes and honey, Laboratory of Functional Anatomy of cultivated plants, Laboratory for Optical Microscopy, Laboratory of Virology and Mycology, Laboratory of Entomology and Agricultural Zoology, Laboratory testing of microclimate in agricultural buildings, Laboratory of the Department of technologies of agricultural products, Laboratory of Physiology of domestic animals, Laboratory for chemical and biological analysis of water histological laboratory, Laboratory for feeding domestic animals, Laboratory for feeding fish, Laboratory for phytobacteriology and Laboratory of Agrochemistry and Plant Physiology. The Faculty of Agriculture is involved and participates in the realisation of the following international projects: IPA CBC Programme: “ZOONE - Zoo Technical Networking for a Sustainable Innovation in Adriatic Euroregion”; TEMPUS: “Improving Academia - Industry Links in Food Safety and Quality”; COST: Assessment of European Agriculture Water use and trade under climate change (EURO- AGRIWAT); EPA: "SEELEGUMES (Sustainable preservation of indigenous South East European legumes and their traditional food and feed products)"; TEMPUS: International joint Master degree in Plant Medicine; FP7: Aquavalens- Microbially safe water for human consumption;FP7: ”Veg-i-Trade”- Impact of climate change and globalisation on safety of fresh produce – governing a supply chain of uncompromised food sovereignty; FP7: AREA – Advancing research in agricultural and food sciences at Faculty of Agriculture – University of Belgrade. Also, the Faculty of Agriculture participates in the implementation of 70 national projects funded by the Ministry of Education, Science and Technological Development of Republic of Serbia (16 projects of

63 TREASURE 634476 integrated and interdisciplinary research, 16 projects in the field of basic research and 38 projects in the field of technological development).

Main tasks in the proposal

WP3. Samples of muscle and fat tissue would be taken for chemical analysis (after slaughtering of pigs in experimental slaughterhouse of the Institute for Animal Husbandry), particularly for the determination of the individual contents of saturated, poly-and monounsaturated fatty acids, cholesterol content, the content of certain heavy metals. The analyses will be done at referent laboratory (Gas chromatography, Atomic absorption spectroscopy).The study would include carcass quality and meat properties of Mangulica and Moravka pigs grown at different sites (lowland and highland). The samples of muscles and various samples of meat products (high quality pork products, dry fermented sausages - Kulen and Sremska sausage). Sausage manufacture will be done at meat processing plant of the Institute.

WP4. Development of Cost-Benefit Analysis of rearing of breeding animals of indigenous breeds (Mangulica and Moravka) in the nucleus (main) herd after performing molecular genetic analysis and confirmation of breed. Cost-benefit analysis of the complete process of production of the finished product (Kulen and Sremska sausages).

WP5. Transfer/exchange of knowledge – at International Congress, publishing of peer-review papers in international journals and national journals, Serbian pig breeders, meat industry. From the results of the project, breeders and meat industry can expect increase in the value of breeding stock in the market who are found to belong to endangered breeds and their offspring; Increasing genetic variability within breeds (reducing the coefficient of inbriding) will enhance the production capacity throat; Preparation of high-quality meat and meat products containing more unsaturated fatty acids compared to saturated, will affect the value of the same (higher price of the product); The activities planned will answer the question of rearing and feeding of fattening pigs indigenous breeds.

Profile of the most relevant staff members who will be undertaking the work

Milica Petrović, PhD EDUCATION 1973 – 1978: Graduated engineer of agriculture, Faculty of Agriculture, University in Novi Sad 1978 – 1982: MSc in the field of genetics and improvement of farm animals, Faculty of Agriculture, University in Belgrade 1983 – 1986: PhD in agricultural sciences, Faculty of Agriculture, University in Novi Sad. Title of PhD thesis: Reproductive traits of Swedish Landrace and its crosses with Large White during life exploitation EXPERIENCES WORK EXPERIENCE 1978 - 1983: Assistant trainee University of Belgrade-Faculty of Agriculture 1983 – 1987: Assistant 1987 – 1993: Docent 1993 – 1998: Associate professor From 1998: Full professor Current Fields of research: The effect of different factors (genetic and environmental) on quality of carcasses, carcass sides and meat of indigenous pig breeds (Moravka and Mangulica) and of meat genotypes.

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Estimation of the breeding value of pigs.

Dragan Radojković, PhD EDUCATION 1984 – 1990: Graduated engineer of agriculture, Faculty of Agriculture, University of Belgrade 1991 – 2000: MSc in the field of genetics and improvement of farm animals, Faculty of Agriculture, University in Belgrade 2001 – 2007: PhD in agricultural sciences, Faculty of Agriculture, University of Belgrade. Title of PhD thesis: Variability of fertility traits and estimation of breeding value of pigs EXPERIENCES WORK EXPERIENCE From 1991: University of Belgrade-Faculty of Agriculture 1991 - 2001: Assistant trainee 2001 – 2008: Assistant From 2008: Docent Current Fields of research: The effect of different factors (genetic and environmental) on quality of carcasses, carcass sides and meat of indigenous pig breeds (Moravka and Mangulica) and of modern pig breeds. Breeding value Estimation of pigs.

Radomir Savić, Msc EDUCATION 2003 - 2006: Graduated engineer of agriculture, University in Pristina, Faculty of Agriculture From 2008: Student of PhD studies, University in Belgrade, Faculty of Agriculture Title of PhD thesis: Phenotypic and genetic variability of fertility traits of the boars (2012, in progress) EXPERIENCES WORK EXPERIENCE Agriculture company “Halas Jožef”a.d., Ada, Vojvodina, Serbia 2007-2008:Farm production (pigs, cattle and sheeps) 2007-2008: Manager of livestock production A. D. “Doža Djerdj”, Bačka Topola, Vojvodina, Serbia 2008-2013: Farm production (pigs) 2008-2009: Manager of Department for reproduction and selection in livestock 2009-2013: Manager of livestock production University in Belgrade- Faculty of Agriculture From 2013: Assistant Current Fields of research: Breeding and reproduction in pigs: male fertility and sperm traits; The effect of different factors (genetic and environmental) on quality of carcasses, carcass sides and meat of indigenous pig breeds (Moravka and Mangulica) and of meat genotypes

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content 1. Petrović Milica, Radović Č., Parunović N., Mijatović M., Radojković D., Aleksić S., Stanišić N., Popovac M. (2010): Quality traits of carcass sides and meat of moravka and mangalitsa pig breeds. Biotechnology in Animal Husbandry, Vol 26, 1-2, p. 21 – 27. 2. Petrović M., Radović Č., Parunović N., Mijatović M., Radojković D., Stanišić N. (2010): Kulen sausage of meat from Mangalitsa and Moravka pig breeds. Technical solution – Significantly improved technology. Biotechnology in Animal Husbandry 26 (spec. issue), p. 81-94. 3. Petrović, M., Č. Radović, N. Parunović, D. Radojković, and R. Savić (2012): Composition of carcass sides and quality of meat from swallow-belly Mangalitsa reared in two systems. Biotechnology in Animal Husbandry, 28, 2, 303-311. 4. Parunović N., Petrović M., Matekalo-Sverak V., Trbović D., Mijatović M., Radović (2012): Fatty acid profile and cholesterol content of M. longissimus of free-range and conventionally reared Mangalitsa pigs. South African Journal of Animal Science, 42, No. 2, 101-113. 5. Parunović N., Petrović M., Matekalo-Sverak V., Radović Č., Stanišić N. (2013): Carcass properties, chemical content and fatty acid composition of the musculus longissimus of different pig genotypes. South African Journal of Animal Science, 43, No. 2, 123-136.

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List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

1. Collection, selection and identification of animals of Moravka and Resavka breeds and work on their preservation (No. 401-00-2100/2004-09; Ministry of Agriculture, Forestry and Water management of Republic of Serbia, project coordinator prof. dr. Milica Petrović, FA); 2. Investigation of slaughter traits and quality of meat products manufactured from meat of Moravka breed pigs", (2006-2008, Ministry of Agriculture, Forestry and Water management of Republic of Serbia, project coordinator prof. dr. Milica Petrović, FA ); 3. Application of molecular-genetic methods in the determination of the genetic variability within and between endangered pig breeds - Mangalitsa and Moravka (ААР007, STAR, project coordinator prof. dr. Milica Petrović, FA); 4. Development and implementation of new biotechnologies for increase of production of quality pig meat (EBP:20087; Ministry of Science and technological development of Republic of Serbia, head of the national project prof. dr. Milica Petrović, FA) 5. Application of various breeding-selection and biotechnological methods in improvement of pigs (BP: 31081; Ministry of Science and technological development of Republic of Serbia, head of the national project prof. dr. Milica Petrović, FA).

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work University of Belgrade-Faculty of Agriculture has a large number of laboratories, equipment for marking carcass, planimeter, PH-meter, apparatus for determining the color of the meat, ultrasound to determine fat thickness, area of MLD, ultrasound of gestation, air box for storage and transport of sperm, software packages for data processing and evaluation of breeding values pigs, cameras, computers.

Partner 20 - INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA)

Description of the organisation The National Institute for Agricultural and Food Research and Technology (INIA) is assigned to the State Secretariat of Research, Development and Innovation of the Ministry of Economy and Competitiveness. As a Public Research Organization it has a dual function, being responsible for the management and coordination of agrifood research at the national level and for the execution of research projects, in close collaboration with the relevant socioeconomic sectors. Within the European level, INIA holds Spain´s representation in those EU forums (SCAR , EIARD , AgriNatura , EURAGRI) where proposals are made to develop and implement an agrifood research scientific policy and has been involved in over 40 EU projects and activities. The INIA team involved in the project belongs to the Pig Breeding and Conservation Research Group of the Animal Breeding Department. The main expertise of the department is the research on genetic conservation and improvement of farm animals, combining classical quantitative genetics tools with new advances in genomics and statistics.

Main tasks in the proposal INIA team will co-lead WP1 (Description and evaluation of genetic resources) which will involve breed characterization using the most innovative technologies for genome, transcriptome and microbiome analyses 1.2, 1.3, 1.4, 1.5, 1.6 and 1.7 tasks. INIA will also be involved in WP2: 2.1, 2.5 and 2.7 tasks and WP3: 3.1 and 3.2 tasks by contributing to the data analysis of phenotypic records and molecular markers. Moreover, in WP5 INIA will be involved in the development of publications, participation on meetings and web and media dissemination. The Pig Breeding and Conservation Research Group focus their research on the applications of genetic markers in pig conservation and breeding, in particular on the molecular authentication of Iberian pigs and their products, and on the genetic architecture of complex and new traits. In recent years INIA, through its center in Zafra which is located in the traditional breed production area, has established partnerships with sectorial organizations to perform applied projects in genetic improvement, traceability and feeding systems and meat quality in close collaboration with the University of Extremadura.

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Profile of the most relevant staff members who will be undertaking the work Dr. Ana I. Fernández is a specialist in molecular genetics technologies applied to animal breeding and conservation and she is currently involved in massive data analyses of whole genome sequencing, RNA-Seq and high density SNP data in different livestock species. She leads and participates in national and European research projects and has a good track in supervising PhD students. Her main areas of expertise include molecular marker development, genome scans, candidate gene analyses and transcriptome and genome analyses. Dr. Juan María Garcia-Casco has broad experience in the development and operation of animal breeding programs specific to Iberian pig. Also, he has worked on the application of molecular techniques for breed authentication and on methods to determine the traceability of feeding systems. He has recently been involved in initiatives related to improving the efficiency and profitability of feeding. Dr. Cristina Óvilo is molecular geneticist and her main research interest is the study of Genetic Basis of productive and reproductive traits in swine. Her research projects are related with the identification of markers, genes and metabolic pathways associated with phenotypic traits. Other research lines include nutrigenomics and prenatal programming studies. Dr. Carmen Rodríguez has more than 25 years of experience in pig research, with special emphasis in the implementation of animal breeding and conservation programs in Iberian pig. She has worked on QTL detection, candidate genes and on the application of molecular techniques for Iberian breed authentication. Dr. Estefânia Alves works on animal genetics research and is proficient in applying molecular biology techniques to the study of the genetic architecture of traits of interest in pigs, such as reproductive and quality traits. In addition, she has participated in several projects focusing on the development of molecular markers for breed characterization and authentication of Iberian pig products genetic origin. Dra. Almudena Fernández is a specialist in the development and application of statistical algorithms for the analysis of molecular data and quantitative traits based on large datasets. She has broad experience in the management genetic conservation programs either with or without the assistance of molecular markers. Dr. Luis Silió has more than 30 years of experience in animal breeding, with particular expertise in conservation of farm animal genetic resources and integration of quantitative and molecular genetics. He has been involved in the direction of research projects and contracts with public and private funding. He also was responsible for the Spanish National Committee for Project Evaluation (Animal Production, Aquaculture and Fishing) between 1995 and 2000, and was Head of the Animal Breeding department (INIA) from 2003 to 2010. Laboratory technicians and assistants: Carmen Barragán, Rita María Benitez, Yolanda Nuñez, Carmen Carabaño and Fabián García have broad experience in molecular genetics techniques and DNA/RNA analyses.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Pérez-Montarelo D., Madsen O., Alves E., Rodríguez M.C., Folch J.M., Noguera J.L., Groenen M., Fernandez A.I. 2013. Identification of genes regulating growth and fatness traits in pig through hypothalamic transcriptome analysis. Physiological Genomics; 00151.2013. 2. Fernández A.I. , Barragán C., Fernández A., Rodríguez MC., Villanueva B. 2014. Copy number variants in a highly inbred Iberian porcine strain. Animal Genetics 45(3):357-66. 3. Rey A.I., Amazan D., López-Bote C.J., García-Casco J.M. 2014. Quantification of γ - and α - tocopherol isomers in combination with pattern recognition model as a tool for differentiating dry- cured shoulders of Iberian pigs raised on different feeding systems. J Sci Food Agric. doi: 10.1002/jsfa.6603 4. Silió L., Rodríguez M.C., Fernández A., Barragán C., Benítez R., Ovilo C., Fernández A.I. 2013. Measuring inbreeding and inbreeding depression on pig growth from pedigree or SNP-derived metrics. J Anim Breed Genet 130: 349-360. 5. Óvilo L., Benítez R., Fernández A., Nuñez Y., Fernández A.I., Rodríguez C., Isabel B., Rey A.I., López- Bote C., Silió L. Longissimus dorsi transcriptome analysis of purebred and crossbred Iberian pigs differing in muscle characteristics. Accepted in BMC Genomics

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

National project S2009/AGR-1704: “New production systems to maximize the meat quality and welfare” (2009-2013)

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National project: RTA2011-00113-00-00: “Application of new genomic tools for Iberian pig selection” (2012-2014) National project AGL2011-29821-C02-02: “Application of Genomics and Massive Parallel Sequencing methods to the study of genetic variants that regulate growth, conformation and meat quality in pigs” (2012- 2014) National project RZ2012-00006-00-00: “Developing a reduced SNP panel for breed authentication of Iberian pigs registered in the Herd book of the Iberian pig breed” (2013-2015) National project: RTA2008-00026-C07-00: “Evaluating the feeding received by Iberian pigs in the final stage of bait using different instrumental analysis techniques. Applicability and reliability” (2009-2012)

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Molecular genetics labs equipped for DNA isolation and treatment, RNA isolation and treatment, PCR, electrophoresis, post-PCR treatments, pyrosequencing and quantitative PCR. HP PROLIANT ML350G6 and PowerEdge T620, Intel® Xeon® E-26XX v2 Servers and CLC Genomics Workbench software for massive sequencing data analyses Iberian pig DNA bank

Partner 21 - AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)

Description of the organisation Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC) is the largest public multidisciplinary research organization in Spain. It has a staff of more than 13,000 employees, among these about 3,300 are permanent researchers and about 4,300 are pre- and post-doctoral researchers. The CSIC has 132 institutes or centres distributed throughout Spain. In addition it has 52 Joint Research Units with universities or other research institutions. There is also a delegation in Brussels. It has considerable experience in both participating and managing R&D projects and training of research personnel. Under the 7th Framework Programme CSIC has signed 724 actions (including 62 coordinated by CSIC and 45 ERC projects). Funding wise, CSIC is listed the 6th organisation in Europe in the 7th Framework Programme. As to the number of projects signed by CSIC within each programme, the distributions is People 36,4%, Cooperation 32,1%, Capacities 25,2% and Ideas 6,3%. If we take into account funding, the ranking would be different: Cooperation 45%, IDEAS 28%, People 19%, Capacities 8%. In addition, CSIC presents a large participation in other European programmes as LIFE+, INTERREG, EMRP, RFCS, ERANET, etc.

Main tasks in the proposal The main tasks that this team will develop in our proposal will be related to the nutrition and metabolism of local pig breeds (Iberian pigs). During the last years, the members of the team have leaded or collaborate in more than 15 projects related to these issues, and have produced more than 40 SCI papers in the field, many of them within the first quartile according to the JCR. We have also presented nearly 100 communications and posters at the most relevant international congress and symposia related to the field (International Symposium of Energy and Protein Metabolism and Nutrition, Digestive Physiology of Pigs, EAAP, International Symposium of the Mediterranean Pig, etc.). We have also directed 8 PhD Thesis in this area. We have contributed to establish nutritional (protein and energy) requirements for growing Iberian pigs from weaning to slaughter, and to characterize the metabolic profile of this obese pig breed. In WP5, CSIC will be involved in the development of publications and participation of meetings, and in web and media dissemination.

Profile of the most relevant staff members who will be undertaking the work

Surname: Nieto Liñán Name: Rosa Mª Nationality: Spanish

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Gender: Female Degree in Biology University of Granada, Spain (1989) Ph.D. in Biology University of Granada, Spain (1994) Postdoctoral Fellow (1994-1996) Rowett Research Institute, Aberdeen, UK Tenured Scientist, 1998, Estación Experimental del Zaidín, CSIC, Granada, Spain Research Scientist, 2008, Estación Experimental del Zaidín, CSIC, Granada, Spain Department: Physiology and Biochemistry of Animal Nutrition

Expertise: Protein and energy nutrition and metabolism of monogastric animals, particularly pigs. Metabolism studies, nutrient flows and monitoring by means of stable isotopes (C13, N15) of metabolic pathways. Participated in more than 20 research projects and contracts on the field (leader of 4). Co-author of 55 SCI papers, more than 15 non SCI papers and more than 50 communications / posters presentations in international congress or symposia. Supervisor or co-supervisor of 7 PhD Thesis in the field.

Surname: Fernández-Fígares Ibáñez Name: Ignacio Nationality: Spanish Gender: Male Degree in Pharmacy University of Granada, Spain (1989) Ph.D. in Pharmacy University of Granada, Spain (1995) Postdoctoral Fellow (1995-1998) Centro de Investigación y Formación Agraria. Conserjería de Agricultura y Pesca. Junta de Andalucía, Granada, Spain Postdoctoral Fellow (1999-2001) USDA-ARS. Beltsville. MD., USA Scientist (2001-2005; Ramón & Cajal Programme), Estación Experimental del Zaidín, CSIC, Granada, Spain Tenured Scientist, 2005, Estación Experimental del Zaidín, CSIC, Granada, Spain Department: Physiology and Biochemistry of Animal Nutrition Expertise: Nutrition and metabolism of monogastric animals, particularly pigs. Cell culture and metabolism: hepatocytes and adipocytes. Metabolic modifiers Participated in more than 20 research projects and contracts on the field (leader of 4). Co-author of 31 SCI papers, more than 10 non SCI papers and more than 50 communications/ posters presentations in international congress or symposia. Supervisor co-supervisor of 4 PhD Thesis in the field.

Surname: Lachica López Name: Manuel Nationality: Spanish Gender: Male Degree in Biology University of Granada, Spain (1988) Ph.D. in Biology University of Granada, Spain (1993) Tenured Scientist (1998-1999), E (Kika) de la Garza Institute for Goat Research, Langston University (OK), USA Postdoctoral Fellow (2000-2001), Estación Experimental del Zaidín, CSIC, Granada, Spain Tenured Scientist (2002), Estación Experimental del Zaidín, CSIC, Granada, Spain Department: Physiology and Biochemistry of Animal Nutrition Expertise: Protein and energy nutrition and metabolism of animals. Metabolism studies, by means of stable isotopes and calorimeters. Participated in more than 20 research projects and contracts on the field (leader of 1) Co-author of 35 SCI papers, 9 non SCI papers and more than 80 communications/posters presentations in international congress or symposia. Supervisor, co-supervisor of 3 PhD Thesis in the field.

Surname: Seiquer Gómez-Pavón Name: Isabel Nationality: Spanish Gender: Female Degree in Pharmacy University of Granada, Spain (1984) Ph.D. in Pharmacy University of Granada, Spain (1991) Postdoctoral Fellow (1992-1993) Unité de recherche sur les dyslipidémies et l`athérosclérose, Hôpital Henri Mondor, Paris, France/Department of Biochemistry and Molecular biology of Malaga University, Spain. University lecturer (1994-1996), University of Jaen, Spain. Researcher, 1997, Estación Experimental del Zaidín, CSIC, Granada, Spain. Tenured Scientist, 2007, Estación Experimental del Zaidín, CSIC, Granada, Spain Department: Physiology and Biochemistry of Animal Nutrition Expertise: Mineral nutrition and metabolism of humans and monogastric animals, mineral bioavailability, food processing and technology, cell cultures, oxidative stress.

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Participated in more than 15 research projects. Co-author of 66 SCI papers, more than 15 non SCI papers and more than 100 communication/ posters presentations in international and national congress or symposia. Supervisor, co-supervisor of 5 PhD Thesis.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Fernández-Fígares, I., Lachica, M., Nieto, R., Rivera-Ferre M.G., Aguilera, J.F. 2007. Serum profile of metabolites and hormones in obese (Iberian) and lean (Landrace) growing gilts fed balanced or lysine-deficient diets. Livestock Science, 110: 73-81. 2. Conde-Aguilera, J.A., Aguinaga, M.A., Aguilera, J.F., Nieto, R. 2011. Nutrient and energy retention in weaned Iberian piglets fed diets with different protein concentrations. Journal of Animal Science, 89: 754-763. 3. R. Nieto, L. Lara, R. Barea, R. García-Valverde, M.A. Aguinaga, J.A. Conde-Aguilera and J.F. Aguilera. 2012. Response analysis of the Iberian pig growing from birth to 150 kg body weight to changes in protein and energy supply. Journal of Animal Science 90: 3809-3820. 4. Gómez-Carballar, F., Lara, L., Nieto, R. and Aguilera, J.F. 2013. Response of the Iberian sow to protein supply and feeding level during late gestation. Animal Feed Science and Technology 181: 72- 79. 5. Gómez-Carballar, F., Lara, L., Nieto, R. and Aguilera, J.F. 2013. Effect of increasing lysine supply during last third of gestation on reproductive performance of Iberian sows. Spanish Journal of Agricultural Research 11: 798-807

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal - Protein and energy requirement for weaned pigs, maintenance energy requirements from birth to slaughter, and relevance of intestinal amino acid absorption of microbial origin (Ref. AGL 2005-01652). National Project (2005-2008). Research leader: R. Nieto -Studies on the efficiency of nutrient and energy utilization and changes in digestive physiology of the Iberian piglet during suckling and post-weaning periods. (Ref. AGR-395). Regional Project (2006-2009). Research leader: J.F. Aguilera -Comparative studies on amino acid and lipid metabolism of the Iberian and a conventional pig breed: effects on growth, nutrient utilization and product quality (Ref. AGL2011-25360). National Project (2012- 2014). Research leader. R. Nieto -Studies on the capacity for protein deposition of the heavy Iberian pig. Research Contract (2014-2015). Research leader: J.F. Aguilera -Addition of natural metabolic modifiers to pig diets to enhance productivity (Ref. RECUPERA2020 3.3.1). National Project (2013-2015). Research leader: I. Fernández-Fígares.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work Our department has facilities related to the studies foreseen in the project, including a barn for housing individually up to 48 growing pigs, and metabolic cages for nutrient flows studies, particularly focused on N. We have also well-equipped labs with current equipment (high precision scales, pH meters, fume cabinets, ovens, etc.) and chromatographs for amino acids (HPLC) and fatty acid analysis (GC). A mass GC-mass spectrometer is also available.

Partner 22 - CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC-IRTA (CREDA)

Description of the organisation The Centre for Research in Agro-food Economics and Development (CREDA, www.creda.es) is a recently created institute participated by the Technical University of Catalonia (UPC) and by the Agricultural Technology Research Institute (IRTA). Researchers at CREDA have participated in quite a few competitive projects both at national and European levels (FP4, FP5, FP6 and FP7). Research interests of the team covers

70 TREASURE 634476 a wide range of topics from the production to the consumption sides. Researchers at CREDA have extensively worked in farm production economics: economic valuation of agricultural externalities, technical and environmental efficiency studies, adoption of innovations, investment and production decisions under public subsidies, decision in uncertainty environment, etc. Most of the researches has been carried out using microdata at farm level. Results from research have been published in a wide range of scientific journals, books or conference proceedings, in the fields of consumer behaviour, agricultural marketing, agricultural economics and agri-business, although special attention has been also paid to the dissemination of results among producers and policy makers. Results from research have been published in a wide range of scientific journals, books or conference proceedings, in the fields of consumer behavior, agricultural marketing, agricultural economics and agri-business, although special attention has been also paid to the dissemination of results among producers and policy makers.

Main tasks in the proposal CREDA will mainly contribute to WP 4. As this WP is closely related to WP2 and 3, CREDA will marginally participate in those WPOs. Taking into account the expertise of CREDA, their tasks will focus on the analysis of the socioeconomic impact of the suggested changes in WP1, 2 and 3 on specific production systems and breeds along the food chain. This analysis will cover a cost-benefit analysis at farm and societal level, acceptability along the food chain and a consumer analysis to better their decision making process related to new products and to evaluate their preferences and willingness-to-pay for specific pork products. CREDA will also deal with dissemination WP5 with organization and participation of meeting.

Profile of the most relevant staff members who will be undertaking the work

José María Gil is a professor of Agricultural markets, agro-food marketing, agro-food market analysis at the Agricultural engineering school of the polytechnic university of Barcelona and Director of the Center for Agro- food Economy and Development-CREDA. He has developed his research activity focusing on price transmission analysis, supply chain studies and food demand and consumer behavior. He has been responsible for several contracts with regional authorities dealing with farms economics. He has recently worked in one project related to the potentialities of defining income insurance in horticulture products. He participated in a project to design a benchmarking tool to help farmers to adopt adequate management strategies. Zein Kallas: Lecturer of Agribusiness management, Agricultural markets, environmental economics at the at the Agricultural engineering school of the polytechnic university of Barcelona and Researcher at the Center for Agro-food Economy and Development-CREDA. He has experience in the economic valuation of non- commodities goods and services (externalities) from agricultural systems; Adoption of organic farming; Production and investment decision under public subsidies in the cereals, oilseeds and protein crops sector in Spain. He also working on agro-food market research and economic valuation methods in analyzing animal welfare aspects and consumer behavior. Lluc Mercadé: is an economist researcher at the Center for Agro-food Economy and Development-CREDA. He has participated in numerous economic studies related to the agricultural sector, for public administration and private companies. He also has experience in research activity. Before joining CREDA, he worked in the Economic Studies Unit of the Ministry of Agriculture of the Government of Catalonia. He has an extensive knowledge of the Catalan agriculture sector and experience in working with the main statistic sources related to the agricultural sector as the Farm Accountancy Data Network (FADN), the Farm Structure Surveys (FSS), and the Economic Accounts for Agriculture (EAA) or input and output prices data. He also has experience in designing questionnaires specifically addressed to agricultural producers and experience in quantitative analysis.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Market potential and quality of meat and meat products from entire males. European perspectives of banning pig castration. BOARMARKET). RTA-2011-00027-C02-01. 2. Study on the improved methods for animal-friendly production, in particular on alternatives to the castration of pigs and on alternatives to the dehorning of cattle ALCASDE. SANCO D5 JK/dj D (2008)450346 (2009-2010). 3. Experimental network, exchange and transfer for the development of organic farming in crop production to Catalan farmers. EFA10/08 REDBIO (2007-2013).

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4. Social and environmental externalities valuation in agricultural sytems. (2004-2006). Funding: Ministerio de Ciencia y Tecnología y el FEDER. MULTIAGRO (AGL2003-07446-C03-01) (2004- 2006). 5. Sustainability analysis of agricultural systems in the PAC frame FUTURPAC. AGL2006-05587-C04- 01/AGRof ((2006-2009).

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

Kallas, Z.; Gómez-Limón, J.A. and Arriaza, M. (2007). “Are Citizens willing to pay for Agriculture multifunctionality?” Agricultural Economics, 36(3) 307-321. Serra, T.; Chambers, R.; Oude-Lansink, A. (2013) “Measuring technical and environmental efficiency in a state-contingent framework” Paper presented at the Agricultural and Consumer Economics Departmental Seminar, the University of Illinois, Urbana, Illinois. 13 de Febrer. Guesmi, B.; Serra, T. (2013). “Technical and environmental efficiency of Catalan arable crop farms” . 13th European Workshop on Efficiency and Productivity Analysis (EWEPA) Conference, Helsinki, Finland. 17 al 20 de Juny. Kallas, Z.; Gómez-Limón, J.A. and Arriaza, M. (2008). “Demand for non-commodity outputs from extensive agricultural systems”. Mediterranean journal of economics, agriculture and environment 7(1): 4-13. Kallas Z.; Gil J.M.; Panella-Riera N.; Blanch M.; Tacken G.; Chevillon N P.; De Roest K. & Oliver, M.A. (2012) Facing new EU policies towards animal welfare improvement. the relative importance of pig castration. Eurochoices, 11(3), 36-43.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

CREDA is in charge of the socioeconomic analysis. No specific infrastructure equipment is necessary. We have the statistical software needed to carry out the analysis in the assigned tasks.

Partner 23 - CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX)

Description of the organisation

CICYTEX (Scientific and Technological Research Center of Extremadura) is a R+D+I public institution of the local government of Extremadura (a southwestern region of Spain) focused on applied research in agriculture, livestock farming, agri-food and forestry. Projects include research and technological transfer to Sectors related to these activities. The main research areas are the following: a) Agriculture and forestry: Extensive farming. Animal production. Extensive livestock production Extensive land use. Forest production (cork ,wood and charcoal). Biodiversity and conservation. Germplasm banking, Phytopathology. Pastures. Irrigation. Fruit growing. Bioenergy. Precision agriculture. b) Agri-food industry: New methods to increase products' life cycle, transformation processes, minimally processed products, post-harvest technologies, enology processes and viticultural techniques, product quality, new products, use of by-products and industrial processes, hydrostatic high pressures for food treatment.

Main tasks in the proposal Francisco I. Hernández García and Mercedes Izquierdo Cebrián, involved in the conduction of research Task 2.4, match the required expertise of this task in relation to “… management practices involving animal welfare and health (e.g. considering new European rules on pig castration applicable to the production of heavy local pigs, immunocastration), and their effects on production traits and product quality “, since they have been working in projects related to this area, as it can be seen later in this document. In relation to this, Dr. Francisco I. Hernandez as a long experience in pig reproduction and have further focused on the development of male and female immunocastration protocols specific for heavy Iberian pigs. Moreover, Dr. Izquierdo has an extended experience in animal breeding and in pig carcass quality and composition measured either in the carcass or in live animals with ultrasound techniques. Furthermore, they performed studies focused in the effect of immunocastration on carcass and meat cut yields in extensively reared Iberian pigs.

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In relation to Task 2.5 “Experiments to evaluate the effect of inclusion of special local feeding resources (e.g. pasture, grass, hay, hop, acorns) and agro-by-products (e.g. olive, milk and dairy by-products, fibre sources) for feeding local pig breeds will be performed”, Dr. J. Matias, the researcher in charge of this task, has a great experience in agricultural by-product valorization, including residues from paddy sector (straw and husk). Thus, today he is the coordinator of an European Project (LIFE: WALEVA) in which valorization of biomass residues from paddy (straw rice) will be studied by converting them into levulinic acid. On the other hand, this researcher has a wide experience in biomass chemical characterization, biochemical conversion and physical transformation (pellets), as described later on. In relation to WP5, CICYTEX will be involved in the development of publications and participation on meetings.

Profile of the most relevant staff members who will be undertaking the work

Francisco I. Hernández-García has a broad experience in animal reproduction. Main areas of expertise: Sperm quality evaluation and cryopreservation. Artificial insemination. Laparoscopic intrauterine insemination in sheep. Utero-ovarian ultrasonography and follicular dynamics in cattle, goats, sheep and swine. Ultrasonographyc evaluation of body condition and body composition traits in swine. Development of estrous induction/synchronization treatments in cows, goats, ewes and pigs. Piglet perinatology. Development of male and female immunocastration protocols specific for heavy Iberian pigs.

Mercedes Izquierdo-Cebrián has ample experience in quantitative genetics, Statistics, in vivo prediction of carcass composition (fat and muscle deposition) of Iberian pigs by using ultrasound techniques. She also has experience in genetic selection for reproductive and maternal traits in ruminants and swine, application of breeding programs for carcass composition, and in the prediction of intramuscular fat by using ultrasound techniques and mathematical models. She also has been working in the study of carcass composition of immunocastrate males and females. Currently she is in charge of the Swine Researh Department of CICYTEX.

Javier Matías has a wide experience in agricultural by-product valorization, including residues from the paddy sector (rice straw and husk). He is currently the coordinator of an European project (LIFE: WALEVA) in which valorization of biomass residues from straw rice will be studied by converting them into levulinic acid. On the other hand, this researcher has a broad experience in biomass chemical characterization, physical transformation (pelleting) and biochemical conversion.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Hernández-García F.I., Juan L. Duarte, Miguel A. Pérez, Cristina Raboso, Ana I. Del Rosario, Mercedes Izquierdo. 2013. Successful long-term pre-pubertal immunocastration of purebred Iberian gilts reared in extensive systems. 8th International Congress on Mediterranean Pig. Ljubljana (Slovenia), October 2013. Acta Agriculturae Slovenica - Supplement 4:123-126. (http://aas.bf.unilj.si/zootehnika/supl/4-2013/cont-suppl4.htm) 2. Izquierdo M, Miguel A. Pérez, Ana I. Del Rosario, Pedro Rodriguez, Javier García, Juan L. Duarte, Antoni Dalmau, Hernández-García F.I. 2013. The effect of immunocastration on carcass and meat cut yields in extensively reared Iberian gilts. 8th International Congress on Mediterranean Pig. Ljubljana (Slovenia), October 2013. Acta Agriculturae Slovenica - Supplement 4:151-154. (http://aas.bf.unilj.si/zootehnika/supl/4-2013/cont-suppl4.htm) 3. D. Ayuso, A. González, F. Hernández, J. M. Corral, and M. Izquierdo. 2013. Prediction of carcass composition, ham and foreleg weights, and lean meat yields of Iberian pigs using ultrasound measurements in live animals. Journal of Animal Science, vol. 91, pp. 1884-1992. 4. Matías, J., González, J, Cabanillas, J, Royano, L. 2013. Influence of NPK fertilisation, clone and harvest date on agronomic performance of Jerusalem artichoke crop in Guadiana Basin (Southwestern Spain). Industrial Crops and Products, Vol: 48, 191- 197 5. Matías, J., González, J, Royano, L, Barrena, R.A. 2011. Analysis of sugars by liquid chromatography- mass spectrometry in Jerusalem artichoke tubers for bioethanol production optimization. Biomass and Bioenergy, Vol 35, 2006- 2012.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

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TITLE: "Animal welfare indicators in extensive production systems of acorn-fed Iberian pigs and alternatives to surgical castration of males and females: consequences on ethological, productive, reproductive and carcass and meat quality traits” (RTA2010-00062-C02-00). FINANCIAL SUPPORT: INIA. DURATION: 2010-2013. - SUBPROJECT II (RTA 2010-0062-C02-02): BUDGET of Subproyect II: 81,468 €. COORDINATOR of Subproject II: Francisco I. Hernández García. Subproject II resulted in the development of two immunocastration protocols for pre-pubertal and adult (pre-finishing) Iberian gilts reared in extensive systems that were 100% effective and kept their effect up to at least 16 months of age.

TITLE “Effect of birth weight litter variation and finishing system on fat and muscular tissue deposition measured with ultrasound, muscle fibre characteristics and carcass and meat quality in purebred and crossbred Iberian pigs”. FINANCIAL SUPPORT: INIA (RTA-2011-00042-00-00). DURATION: 2012-2015. BUDGET: 44.900 €. COORDINATOR: Mercedes Izquierdo Cebrián

TITLE: “Evaluation of real-time ultrasonography as a non-invasive technology for the determination of carcass composition traits in iberian pigs". FINANCIAL SUPPORT: INIA (RTA-2007-000-93-00-00). DURATION: 2008-2010. BUDGET : 54,024 €. COORDINATOR: Mercedes Izquierdo Cebrián

TITLE: “Improvement of agricultural systems and tailoring of crops to optimize the competitiveness and sustainability of farms”. FINANCIAL SUPPORT: Operational Programme for Cross-border Cooperation: Spain- Portugal, 2007-2013. AGRISOS: 0450-AGRI-SOS-6-E Coordination: Agricultural Technological Institute of Castilla y León and Javier Matías.

TITLE: “From Whatever Residue into Levulinic Acid – an innovative way to turn waste into resource”. FINANCIAL SUPPORT: LIFE Programme WALEVA LIFE13 ENV/ES/001165-WALEVA. DURATION 2014-2017. Coordination: Técnicas Reunidas S.A., and CICYTEX coordinator: Javier Matías.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

Swine research farm: 800 hectars of “dehesa” rangeland (sparsely forested, Mediterranean grasslands of Southwestern Iberian peninsula) for the montanera (free-ranging acorn feeding) of Iberian pigs. Swine maternity, artificial insemination and electronic feeding units. Special corrals with video-surveillance for animal welfare and reproductive behavior observation of Iberian pigs. Ultrasound study unit and image analysis lab. Animal reproduction lab: Complete equipment for reproductive and body composition ultrasonography and image analysis in ruminants and swine. Phase-contrast, fluorescence microscope for seminal analyses. Computer-Aided Semen Analysis (CASA) system. Equipment for semen evaluation and cryopreservation. Animal genetics lab: Freezers (-80ºC; -28ºC). PCR, SSCP and RT-PCR equipment. DNA-sequencer. Electrophoresis equipment. Vegetal material resources: Inductively coupled plasma optical emission spectrometry (ICP-OES) (Varian 715- ES). Microwave Digestion System (Milestone Start D). TruSpec CHNS Carbon/Hydrogen/Nitrogen/Sulphur Elemental Analyzer (LECO). Liquid chromatography–mass spectrometry (Varian MS 310 TQ). Semi industrial pelleting machine (100 kg/hour).

Partner 24 - INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA)

Description of the organisation

IRTA is a research institute owned by the Government of Catalonia ascribed to the Department of Agriculture. It is regulated by Law 04/2009, passed by the Catalan Parliament on 15 April 2009, and it is ruled by private regulations. The institute has more than 500 employees with the main aim to contribute to the modernization, competitiveness and sustainable development of agriculture, food and aquaculture sectors, the supply of healthy and quality foods for consumers and, improving the welfare of the population. The target of IRTA is to contribute in research, technology transfer and dissemination in international projects, focusing in

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Europe but open to working with America, Asiatic and Soviet countries either with public or private founding. The Animal Breeding and Genetics Program of IRTA is a scientific team focused on the study and selection of economically and socially important traits in animal production systems, as well as on the conservation and breeding improvement of swine and other animal genetic resources. Regarding Pig Breeding and Genetics, during the last 5 years the Program has leaded more than 15 research competitive projects or R+D contracts with a shared objective of increasing sustainability of pig production systems by improving pork quality, feed efficiency and robustness. The Product Quality program of IRTA has long experience on research on meat quality - technological, sensorial and nutritional – in relation to animal genetics, diet and ante and post- mortem treatments. The group has also worked on consumer studies on the field of preferences and acceptability and has leaded or coordinated national and international projects. It has collaborated with private companies of the pig and meat sector since its creation.

Main tasks in the proposal

IRTA will mainly participate in WP1 (Genetic resources), WP3 (Quality of products), WP4 (Consumer preferences and market research) and WP5 (Organization of a workshop and web and media dissemination).

Dr. Maria Angels Oliver is the Coordinator of the IRTA-Product Quality Program. She is a senior scientist with an international background in the field of meat quality (technological, nutritional and sensorial quality) and its relation with genetic and sustainability. She has wide expertise on consumer research (acceptability and attitudes) on pork (boar taint, genetics, diet) and beef (genetics, diet). She has participated either as coordinator or WP leader in international projects (ALCASDE, PIGCAS, YOUNG TRAIN, OTRAC, CAMPIG, BOARCHECK), and has more than 60 scientific publications (h index=26). Also she has been a lecturer in several Universities and at Congresses inside and outside Europe. She will be the leader of WP4 and will participate in WP3 as leader of Task 3.3. Dr. Maria Font i Furnols is the Director of the Carcass Quality Subprogram at IRTA. She is a senior scientist and has published more than 45 scientific publications with a strong background on boar taint research, consumer studies and statistics. Since 1995, she has participated in projects related to boar taint and consumer research (FP5 – AIR, FP6 - PIGCAS -, tenders from SANCO – ALCASDE - and recently CAMPIG, where she was responsible to conduct the statistical analysis of the consumer studies done in five European countries and in China and Russia). She will participate in WP3 and WP4. Dr. Marta Gil is researcher at the Product Quality program. She is a senior scientist with background in meat quality (tenderness, sensory, aging) and its relationship with muscle biochemistry and in relation to genetics and diet. She has worked on studies of characterization of meat from local breeds and has published more than 25 scientific articles and participated in more than 15 international (FP6, FP7) and national research projects. She will participate in WP3 and WP4.

Dr. Raquel Quintanilla is the Coordinator of the IRTA-Animal Breeding and Genetics Program. She has more than 15 years-experience as animal genetics researcher and professor at Spanish and French Institutions. Expertise in statistical genetics & genomics focused to the study of genetic mechanisms underlying economically and socially important traits in pigs. She has leaded three Spanish National projects on genetics of pigs’ lipid metabolism and pork quality, and has participated in other ten Spanish and European projects plus several R+D contracts with companies. She has (co)authored more than 45 scientific publications (630 citations) in SCI journals. She will participate in WP1. Dr. Joan Tibau i Font is a senior scientist at the Animal Breeding and Genetics Program, with close to 30 years as animal genetics professor at the Girona University, and involved in 40 research contracts and several Spanish and EU public founded projects. Topics: optimization of breeding programs (including the use of “omics” and re(pro)duction technologies) , genotype characterization, sustainable animal production systems, assessment of environmental impact of meat production (including processing) and qualitative characterization (and promotion) of meat and meat products . He will participate in WP1

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

1. Font-i-Furnols, M., Tous, N., Esteve-Garcia, E., Gispert, M. (2012) Do all the consumers accept the marbling in the same way? The relation beetween visual and sensory acceptability of pork. Meat Science, 91, 448-453. 2. Gonzàlez, J., Jaume, J., Fàbrega, E., Gispert, M., Gil, M., Oliver, A., Llonch, P., Guàrdia, M.D., Realini, C. E., Arnau, J., Tibau, J. (2013) Majorcan Black Pig as a traditional pork production system:

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Improvements in slaughterhouse procedures and elaboration of pork carpaccio as an alternative product. Meat Science 95, 727-732 3. Oliver, M.A., Nute, G.R., Font i Furnols, M., San Julián, R., Campo, M.M., Sañudo, C., Cañeque, V., Guerrero, L., Alvarez, I., Díaz, M.T., Branscheid, W., Wicke, M., Montossi, F.(2006). Eating quality of beef from different production systems, assessed by German, Spanish and British consumers. Meat Science 74 435-442. 4. Panella-Riera, N., Gispert, M., Gil, M., Soler, J., Tibau, J., Oliver, M.A., Fabrega, E. 2012 Effect of feed deprivation and lairage time on carcass and meat quality traits on entire male pigs. Livestock science, 146: 29-37. 5. Pena R., D. Gallardo, M.D. Guàrdia, J. Reixach, J. Arnau, M. Amills, R. Quintanilla. (2013) Appearance, flavor and texture attributes of pig dry-cured hams have a complex polygenic genomic architecture. Journal of Animal Science 91(3):1051-1058

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

- Q-PORK-CHAINS Contract FP6-036245-2, 2007-2011 – Improving the quality of pork and pork products for the consumer: Development of innovative, integrated, and sustainable food production chains of high quality pork products matching consumer demands. - OTRAC, INTERREG, 2009-2012 - Development of a cross-border organization breed/ territory/product from Genetics to Meat of Quality for a sustainable economic development in the Pyrenees area (Gascony bovine breed model). - ALCASDE, SANCO/D5/2008/SI2.517191, 2009 - Study on the improved methods for animal- friendly production, in particular on alternatives to the castration of pigs and on alternatives to the dehorning of cattle. - ECO-FCE, FP7-311794, KBBE-2012-6, 2013-2016 - A whole systems approach to optimising feed efficiency and reducing the ecological footprint of monogastrics. - CAMPIG, SANCO/2012/G3/SI2.639774, 2012-2014 - Consumer acceptance in EU and 3rd countries of meat from non-surgically castrated male pigs.

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

- NIRS Bruker Optics Matrix-F duplex spectrometer with several fibre optic probes. - Helical Computed Tomography equipment, General Electric, model Hi Speed Zx/I

Partner 25 - AECERIBER (AECERIBER)

Description of the organisation

Spanish Association of Iberian Pig Breeders. National non-profit entity, with the objectives of promotion, dissemination, defense and selection of the Iberian porcine breed. It has been recognized as a Pure Breed association by the EU and has the tasks of developing the Genealogic Book of the Iberian breed and to perform the official selection of the breed. It comprises more than 700 farmers and a total of 1500 Iberian pig farms. It is considered the sectorial representative for the regional authorities and the Ministry of Agriculture, Feeding and Environment.

Main tasks in the proposal The task developed by our organization in the case of the project succeed, will involve the collection of productive trait data from different strains of the Iberian breed and to participate in diffusion events. We regularly organize workshops among Iberian pig breeders and contribute to the diffusion of technical knowledge through the publication of our journal “Solo cerdo Ibérico” twice a year, as indicated below.

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We can contribute to the diffusion of relevant production practices through local fairs (Zafra, Salamanca, Pozoblanco, Spain) and will be able to provide productive data form our associated breeders. AECERIBER will be especially involved in WP5 dissemination: Task 5.1 - publications and dissemination in newsletters, participation on meetings and workshops, organization of meetings. Promotion of TREASURE at meeting and events organized by FESERPAE (European Autochthonous Extensive Porcine Breeds Federation), and workshops organized by ASICI (Inter-professional association of the Iberian pig) and at local fairs of Pozoblanco, Salamanca and Zafra (Spain) Task 5.4 – transfer of knowledge (training workshops and events, demonstration in local fairs)

Profile of the most relevant staff members who will be undertaking the work

The personal undertaking the work will be Technical and administrative staff belonging to the association and with a high or medium university Degree.

Name: Elena Surname: Diéguez Garbayo Gender: Female Born in Madrid, Spain. Degree in Veterinary (UCM, 1982, Madrid, Spain) Manager of AECERIBER since 1987 Vicepresident of DPO “Dehesa de Extremadura” since 2007 President of European Autochthonous Extensive Porcine Breeds Federation (FESERPAE), since 2004.

Name: Ana Surname: Sánchez Palop Gender: Female Born in Zafra, Badajoz, Spain. Degree in Direction and Administration (University Salamanca, Spain, 2001) Responsible for the General Admistration of AECERIBER since 2006.

List of up to 5 relevant publications, and/or products, services (including widely-used datasets or software), or other achievements relevant to the call content

 Elena Diéguez Garbayo. El sector del Ibérico se enfrenta al reto de una nueva norma de calidad. Tierras de Salamanca. August, 2013.  Elena Diéguez Garbayo. El sector del Ibérico y el congreso mundial del jamón. 2011. Cárnica 2000, 83: 25-26  Elena Diéguez Garbayo. ANÁLISIS SECTORIAL DEL CERDO IBÉRICO: Norma de Calidad, Censos y Precios. Solo cerdo Ibérico, April 2013: 63-82.  Elena Diéguez Garbayo. ANÁLISIS SECTORIAL DEL CERDO IBÉRICO: Norma de Calidad, Censos y Precios. Solo cerdo Ibérico, October 2013: 101-106  Elena Diéguez Garbayo. ANÁLISIS SECTORIAL DEL CERDO IBÉRICO: Norma de Calidad, Censos y Precios. Solo cerdo Ibérico, April 2014: 47-57.

List of up to 5 relevant previous projects or activities, connected to the subject of this proposal

Organization of difussion events: Workshop on updating pig farms is regularly organizated by us (Jornadas de Modernización de las explotaciones de cerdo Ibérico”.

Web page of the association: http://www.aeceriber.es/

Publication of the journal Sólo Cerdo Ibérico (Only Iberian pig), twice a year (April, October)

Description of any significant infrastructure and/or any major items of technical equipment, relevant to the proposed work

AECERIBER will participate in collection of breed’s data and in dissemination and transfer of knowledge activities: no specific infrastructure equipment is necessary.

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2.3.4.2. Third parties involved

Partner 1 - KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS)

Partner 2 - UNIVERZA V LJUBLJANI (UL)

Partner 3 - Kmetijsko gozdarska zbornica Slovenije, Kmetijsko gozdarski zavod Novo mesto (KGZS-NM)

Partner 4 - IFIP - INSTITUT DU PORC (IFIP)

Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

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Partner 5 - INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

Partner 6 - Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project Y should not be sub-contracted) We will subcontract certain tasks of our activity within the project. • Tasks 1.1 /2.1 /3.4 herdbook data collection from the pigs for the tests Does the participant envisage that part of its work is performed by linked third parties Y THE BESH AG (PIC: 938385955) is a shareholders company and daughter organisation from the BESH w.V. – BESH w.V. holds about 60% from the shares of BESH AG. The work from BESH w.V. and BESH AG is quite close. BESH w.V. handles in general the organisational parts and is in charge for the living animals and slaughtering. BESH AG is doing the meat processing. Only parts from the tasks will be performed by the BESH AG. • Tasks 1.1 /3.1 /3.4 Meat quality sample collection on slaughterhouse level • Tasks 3.1 / 3.4 Meat quality testing with certain tests • Task 5 Marketing / Brand development / Conference Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 7 - SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG)

Partner 8 - SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEK (PFOS)

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Our third party will be a non-academia organisation, namely “Agricultural service cooperative – LETA” (association Crna slavonska (Black Slavonian) breed). Leta is agricultural service cooperative founded in September 2013 in Vukojevci (near Osijek).. It was established by 9 initial founders, which are also the members. The director of this organisation is dr.Vladimir Margeta. The basic activity of the organisation is the breeding of Black Slavonian pigs, indigenous Croatian breed. The members of association possess 110 hectares of land (mainly grassland and pastures), 130 heads of pigs. The members of association are involved in projects such as “Sustainable production system in the breeding of Black Slavonian pigs” whose leader Martina Petrović, the member of the association, won the first prize “The best young agricultural producer” which is a recognition of the efforts in the care shown in preservation of indigenous breed and the traditional values in agriculture. PFOS has a legal agreement with the cooperative for research and development and promotion of the breed In TREASURE project, LETA supply experimental animals, feed, and accommodation for animals involved in the research. It will also support PFOS in demonstration activities. It will thus be involved in tasks WP1.1, WP2.1, WP2.4, WP3.1, WP3.2, WP5.2, WP5.3, WP5.4. Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 9 - AGRIS Sardegna (AGRIS)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project N should not be sub-contracted) If yes, please describe and justify the tasks to be subcontracted Does the participant envisage that part of its work is performed by linked third parties Y University of Sassari, Veterinary Medicine Department (UNISS), Sect. Inspection of Food of Animal Origin (contact person: Rina Mazzette): its competences are focused on food safety and food hygiene, including researches on chemical and microbial contaminants, microbial prevalence and molecular characterization (PCR, PFGE, RT-PCR, microarray), virulence profile, persistence and traceability of pathogens, and shelf-life studies of fresh meat and meat products (traditional and updated) from autochthonous breed chain. UNISS-VMD and AGRIS have a legal relationship, since 2012, for a Joint Research Unit (Centre for Animal Biodiversity), legally recognised by a public authority (notary public), and economically supported by Sardinia Region with European funds. The foreseen tasks performed by UNISS-VMD, as linked third party, will allow to achieve specific objectives, concerning the training and dissemination activities regarding WP5. Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 10 - Associazione Nazionale Allevatori Suini (ANAS)

Partner 11 - UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI)

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The Consorzio di Tutela della Cinta Senese (CONCS) was set up in 2000 with the objectives of safeguarding the Cinta Senese, an ancient Italian local pig breed, reared in Tuscan region. Since 2012, when the PDO on Cinta Senese meat was approved, it is the official body in charge for safeguarding, protecting and promoting the Designation of Origin of "Cinta Senese" fresh meat. Nowadays the Consortium associates 116 producers of Cinta Senese meat. CONCS is a third party linked to UNIFI by an official agreement. CONCS is essential for UNIFI in carrying out the studies on Cinta Senese in terms of: knowledge, animal and structures supply, transfer of results. In Treasure, CONCS will be involved in the following tasks: Task 3.1 A toolbox will be developed for the evaluation of sensory, nutritional and technological meat quality traits. For each quality trait, the toolbox will include common indicators by pork product categories and specific threshold values for acceptability according to local chain and product. Since the toolbox will be developed based on the expertise and “know-how” of the chain actors, the the knowledge of the CONCS will be crucial in order to achieve the goals of this task. Task 5.4 demonstration and transfer of knowledge Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 12 - Centri Regionali per le Tecnologie Agroalimentari (Ce.R.T.A. scarl) (CERTA)

Partner 13 - ALMA MATER STUDIORUM-UNIVERSITA DI BOLOGNA (UNIBO)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project Y should not be sub-contracted) Subcontracting is foreseen to cover activities of resequencing pig genomes from DNA pools using the latest technologies available in the market when this activity will be needed for the project considering the throughput, read length, error rate an other technical parameters that are important to successfully complete tasks and obtain reliable results for WP1 taking into account the principle of "best value for money". Next generation sequencing technologies are rapidly changing and this is commonly known by scientists working in this field. The participant has next generation sequencing technologies but it does not have the latest ones already available in the market. Moreover, as mentioned before, as additional improvements might be expected in a quite short period of time, the participant cannot assure that it will be possible to acquire and have the newest technologies in house to satisfactorily guarantee to the project the "best value for money" for next generation sequencing activities. Therefore, we envisaged subcontracting activities to specialized service providers (to be appointed as soon this activity will be needed for the project) that might guarantee the lowest cost for resequencing activities (respecting the best technical parameters for the obtained reads) and, in this way, guarantee to the project the principle of "best value for money”. Does the participant envisage that part of its work is performed by linked third parties N If yes, please describe the third party, the link of the participant to the third party, and describe and justify the foreseen tasks to be performed by the third party Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

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Partner 14 - STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA)

Partner 15 - LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS)

Partner 16 - INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project N should not be sub-contracted) If yes, please describe and justify the tasks to be subcontracted Does the participant envisage that part of its work is performed by linked third parties Y ANCSUB (third party) and IPVC (partner) are located in the same geographical area (North Portugal) and have an established collaboration legal agreement. Indeed, from past both institutions have collaborated in scientific/field studies, final student projects developed in farms, and events organization for dissemination/evaluation of local products. ANCSUB (third party) will be responsible for: a) Collecting biological samples from their associated producers for genetic characterization (WP1) and meat samples for quality evaluation (WP3); b) Collecting data about farm and product management practices (WP4); c) Collaboration in dissemination and demonstration activities through expositions fairs about meat products (e.g. Vinhais-North Portugal) and promote visits with the producers to the IPVC experimental pig system (WP5). Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 17 - UNIVERSIDADE DE EVORA (UEVORA)

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The third party ANCPA (National Alentejano Pig Breeders Association) head office is located in the same city (Évora) of the partner (University of Évora, UEVORA) and provides technical support to Alentejano pig breeders and it’s responsible (in coordination with another association) to manage the Herd book of the breed. There is collaboration protocol between the UEVORA and ANCPA that involves collaboration both in the education (students training) and research. ANCPA will collaborate in biological samples and data collection in several tasks. ANCPA will also provide information (eg. Genealogical) regarding on the studied/sampled animals and will be the link to the Alentejano pig farms both for their agreement and collaboration on the studies or data collection. ANCPA will also collaborate in disseminations and demonstration activities (WP5). It will thus be involved in tasks WP1.1, WP2.1, WP2.4, WP5.1, WP5.2, WP5.3, WP5.4. Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 18 - Institut za stočarstvo, Beograd-Zemun (IAH)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project N should not be sub-contracted) If yes, please describe and justify the tasks to be subcontracted Does the participant envisage that part of its work is performed by linked third parties Y Our linked third party is the Association “Eco Village”, Dudovica, Serbia. “Eco Village” Association was founded in 25.04.2010, being its main objectives the following: - to raise indigenous pig breeds (Mangulica and Moravka); - to improve production, processing and marketing of traditional agricultural products; - to drive rural tourism. Every year “Eco Village” Association exhibits indigenous pig breeds at the Agricultural Fair in Novi Sad (Serbia). IAH and Eco Village have an established legal agreement. Eco Village will provide autochthonous pig breeds (Mangulica and Moravka) and will be involved in the dissemination and transfer of technology (i.e. immunocastration, meat products technology, etc.) It will thus be involved in tasks WP1.1, WP2.1, WP2.4, WP2.5, WP5.1, WP5.2, WP5.3, WP5.4. Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 19 - FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project N should not be sub-contracted) If yes, please describe and justify the tasks to be subcontracted Does the participant envisage that part of its work is performed by linked third parties Y Out third party is the Institute of Meat Hygiene and Technology with which we have an established cooperation agreement. It is a research-development institution that organizes and conducts scientific research work of an interdisciplinary character in the field of biotechnology. Research results are applied and implemented during their production, in cooperation with meat and food industry operators. Control of food safety and quality is carried out in the Laboratory for Biotechnological Research, Food Safety and Quality Investigation. Laboratory testing of samples comprises microbiological, parasitological, immune- enzymatic, molecular-biological, physical-chemical and organoleptic (sensory) investigations, and includes all tests stipulated in relevant regulations on safety and compliance and regulations on quality with which individual foodstuffs must comply. Decision of the European Commission "Commission Regulation EC 1168/2002" from the 2002nd year, the Institute of Meat Hygiene and Technology is authorized institutions for the issuance of certificates of authenticity for the export of beef from the Republic of Serbia to the European Union. For TREASURE project the Institute of Meat Hygiene and Technology will perform analyses of meat and meat products (WP3.1, WP3.2) and participate in co-organisation of dissemination events (e.g. conference) in WP5. Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement)

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If yes, please describe the third party and their contributions

Partner 20 - INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA)

Partner 21 - AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project Y should not be sub-contracted) We will subcontract certain tasks of our activity within the project. We will organize an experiment with lactating sows for which our Institute we have no facilities to work with. Therefore, we will perform the experiment in a commercial farm from a private company that has the appropriate facilities to do this kind of experiments. So, although the experiment will be designed and developed by our team, we will use the facilities of this private company and they also will provide us with advice in the management of the experimental animals. The subcontracting costs are 3000 EUR. Does the participant envisage that part of its work is performed by linked third parties N If yes, please describe the third party, the link of the participant to the third party, and describe and justify the foreseen tasks to be performed by the third party Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 22 - CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC-IRTA (CREDA)

Partner 23 - CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project N should not be sub-contracted) If yes, please describe and justify the tasks to be subcontracted

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Does the participant envisage that part of its work is performed by linked third parties N If yes, please describe the third party, the link of the participant to the third party, and describe and justify the foreseen tasks to be performed by the third party Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 24 - INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA)

Does the participant plan to subcontract certain tasks (please note that core tasks of the project Y should not be sub-contracted) The Majorcan Black Pig Breeders Association will be subcontracted to collaborate, jointly with IRTA on data collection (WP2), on data recording for the phenotypic characterisation (WP1) and on the characterisation of the production systems (WP2). The organisation's main role will be to facilitate the contacts with farmers and pave a way for a positive and fruitful collaboration between them and the research team, which is crucial because farmers are usually reluctant to external colaborations. Their tasks will also include a collaboration in identifying and collecting biological samples from representative animals (housed outdoor) for genetic analyses. Does the participant envisage that part of its work is performed by linked third parties N If yes, please describe the third party, the link of the participant to the third party, and describe and justify the foreseen tasks to be performed by the third party Does the participant envisage the use of contributions in kind provided by third parties (Articles N 11 and 12 of the General Model Grant Agreement) If yes, please describe the third party and their contributions

Partner 25 - AECERIBER (AECERIBER)

2.3.5. Planned use of resources Table 3.4b: ‘Other direct cost’ items (travel, equipment, other goods and services, large research infrastructure) 1-KIS10 Cost (EUR) Justification Travel 7,600.00 project meetings, local travel costs for experiments, dissemination activities Equipment Other goods and services 21,000.00 consumables for experimental costs and analysis; publications/dissemination Total 28,600.00

2-UL Cost (EUR) Justification Travel 4,200.00 General Assembly (4x) and WP meetings (2x), dissemination activities Equipment

10- partners for which »other direct costs« do not exceed 15% of personnel costs

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Other goods and services 11,000.00 SNP chip analysis, qPCR, sequencing software; publications Total 15,200.00

3-KGZS-NM Cost (EUR) Justification Travel 5,300.00 project meetings, travels for activities, dissemination activities Equipment 1,000.00 scale for weighing pigs Other goods consumables for experiments (WP2) and costs of WP5 activities incurred by and services 8,000.00 the beneficiary and by the third party Total 14,300.00

4-IFIP11 Cost (EUR) Justification Travel 4,200.00 project meetings, dissemination activities Equipment Other goods and services 5,000.00 Sensory and laboratory analyses Total 9,200.00

5-INRA12 Cost (EUR) Justification travels for project meetings and for sampling, data collection, and consumer studies (6 researchers from 3 different INRA locations involved in the project), Travel 13,300.00 for dissemination activities Equipment DNA sequencing (microbiome), chemicals, small lab goods, small computer Other goods supplies, consumer studies, focus group organization, and services 23,100.00 publications/dissemination Total 36,400.00

6-BESH Cost (EUR) Justification Travel 5,700.00 Project meetings, farm visits, dissemination activities Equipment Other goods Costs for meat & DNA samples, feed and feed analsyses, Conference Venue and services 5,500.00 Place & technique; publications/dissemination Total 11,200.00

7-UNIZG Cost (EUR) Justification Travel 6,500.00 project meetings, local travels for field work, dissemination activities Equipment Other goods Chemicals, consumables for experiments, third party costs, and services 7,150.00 publications/dissemination Total 13,650.00

8-PFOS Cost (EUR) Justification Travel 4,200.00 project meetings, local travels for field work, dissemination activities Equipment Other goods Chemicals, consumables for experiments, third party costs, and services 9,100.00 publications/dissemination Total 13,300.00

11-12 partners for which »other direct costs« do not exceed 15% of personnel costs

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9-AGRIS Cost (EUR) Justification Travel 5,200.00 General assembly, WP meetings, local travel costs, dissemination Equipment Other goods and services 7,500.00 costs of organization of dissemination events Total 12,700.00

10-ANAS Cost (EUR) Justification Travel 4,200.00 project meetings Equipment Other goods and services 3,000.00 goods for biological sample collection; organisation of workshops Total 7,200.00

11-UNIFI Cost (EUR) Justification Travel 5,600.00 project meetings, local travels for project work, dissemination activities 8,000.00 1) Mini server to store and analyze data 2) Fences for the stables employed Equipment to carry out the trial described in task 2.2 Other goods 63,600.00 experimental animals and foodstuffs for trials; chemicals for task 3.2 3; and services recruitments of consumers and costs of publications, dissemination Total 77,200.00

12-CERTA13 Cost (EUR) Justification Travel 5,700.00 travels for meetings Equipment Other goods and services 5,350.00 administrative consumables Total 11,050.00

13-UNIBO Cost (EUR) Justification Travel 8,300.00 General assembly, scientific meeting, farm sampling Equipment Other goods material for resequencing and verification activities in house and publication and services 42,000.00 in open access scientific journals Total 50,300.00

14-SSICA Cost (EUR) Justification Travel 2,800.00 Project meetings Equipment Other goods promotional material and services, material for laboratory activities, external and services 5,500.00 expertise Total 8,300.00

15-LUHS Cost (EUR) Justification Travel 4,920.00 project meetings, local travels for field work, dissemination activities Equipment Other goods consumables for experiments (animals, feed, chemicals …), publications and and services 11,002.00 dissemination activities Total 15,922.00

13 partners for which »other direct costs« do not exceed 15% of personnel costs

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16-IPVC Cost (EUR) Justification Travel 4,200.00 General assembly meetings, WP meetings, dissemination activities Equipment 3,800.00 Feeding experiments: hoop barn; Other goods Costs of animals, feeding, transport between farms and processing plants, and services 24,720.00 dissemination, slaughterhouse service, publications and dissemination Total 32,720.00

17-UEVORA Cost (EUR) Justification Travel 7,184.00 travels for project meetings and data/samples collection, dissemination Equipment Other goods costs of animals, transportation, feeding, chemicals, materials for publications and services 26,732.00 and dissemination activities Total 33,916.00

18-IAH Cost (EUR) Justification Travel 4,200.00 project meetings; local travels for project, dissemination Equipment Other goods consumables for feed, chemicals, sample colleting, raw material for sausages; and services 15,550.00 costs of publications and dissemination activities Total 19,750.00

19-UNIBG Cost (EUR) Justification Travel 4,200.00 GA, WP meetings, local travel for project, dissemination Equipment Other goods consumables for chemicals, sample colleting, costs of publications and and services 4,200.00 dissemination activities Total 8,400.00

20-INIA Cost (EUR) Justification project meetings; sampling in farms (tissues and faecal samples); for Travel 8,200.00 dissemination Equipment Material for sampling and DNA/RNA isolation (cryotubes, RNA later, liquid Nitrogen) for DNA and RNA analyses included in tasks of WP1cca. 6,000€. INIA will conduct the genotyping with the Porcine 60K BeadChip (≈100€ per chip) of samples from all the breeds and genotyping of major gene polymorphisms (> 800 samples) cca. 84,000€. INIA will conduct the transcriptome analyses in 4 different experiments (40 animals; 5 vs 5 for each experiment) for tasks in WP1 through RNA-seq technology cca. 22,000€, additional qPCR validations would also be performed cca. 6,000€. Material for sampling collection, animals, food and chemicals for tasks in WP2 and 3. INIA will take the responsibility of submitting at least Other goods two publications in Open Reading Journals of a part of the main results of and services 135,000.00 the project cca. 2,000€) Total 143,200.00

21-CSIC Cost (EUR) Justification Travel 4,200.00 Travelling cost for consortium and scientific meetings, for dissemination Equipment Other goods Cost of animals, feeds and chemicals necessary to perform experiments and and services 20,470.00 analytical work. Publication and dissemination costs. Total 24,670.00

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22-CREDA Cost (EUR) Justification Travel 9,100.00 project meetings, dissemination Equipment Other goods Field work, consumer studies, focus groups. Publication and dissemination and services 25,400.00 costs. Total 34,500.00

23-CICYTEX Cost (EUR) Justification Travel 4,200.00 project meeting, dissemination Equipment Other goods Consumables to perform experiments and analytical work. Publication and and services 5,000.00 dissemination costs. Total 9,200.00

24-IRTA Cost (EUR) Justification Travel 13,290.00 travels for project meetings, experiments, dissemination activities Equipment Meat from Majorcan black pigs, disposable plastic material, laboratory and pilot plant (15,000 EUR), consumables for laboratory analysis, chemicals and glassware (5,000 EUR), recruitment of 120 consumers, renting premises in Barcelona (4,500 EUR), material (meat, plasticware, questionnaires) for consumer tests (2,600 EUR), renting of premises in Barcelona for choice experiment(4,000 EUR), material for choice experiment (1,500 EUR), funding for attendance of 10 key stakeholders from traditional pork chains to the New Collective Trademark meeting in Germany (6,000 EUR), material for the Workshop (for 50 attendees) on new marketing strategies for traditional pork products: documentation, coffees, meals (2,800 EUR), material for data Other goods collection and sampling (1,500 EUR), laboratory consumables for biological and services 44,400.00 samples processing and conservation (1,5000 EUR) Total 57,690.00

25-AECERIBER Cost (EUR) Justification Travel 2,800.00 meetings within the consortium Equipment Other goods Materials for data collection (WP2) and for organization of events within and services 3,000.00 WP5 Total 5,800.00

2.3.6. Ethics and security 2.3.6.1 Ethics TREASURE project is focused on the study of different European local pig breeds, to enhance available knowledge at phenotypic, genomic and functional level in order to promote a more sustainable pig production system, in regard to environment, animal welfare and food quality and healthiness.

All animals (pigs) will be kept and treated in accordance with standard farm practice and abattoir routines, respecting national and/or EU legislation. Samples will be collected by specialized technicians both on live (e.g. blood) or after slaughter on dead animals, from different tissues. Sample collection will be performed according to national regulations of each country and taking care of the well-being of animals, as described below. Partners of TREASURE will conform to EU legislation concerning protection of farming animals, and within them the followings:  Council Directive 98/58/EC of 20 July 1998, concerning the protection of animals kept for farming purposes and successive amendment and correction (Regulation (EC) No 806/2003)

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 Council Directive 2008/120/EC of 18 December 2008 laying down minimum standards for the protection of pigs.

Pigs of local breeds, the object of this project, are extensively farmed, they benefit from an environment corresponding to their needs for exercise and investigatory behavior, in agreement with their physiological and ethological needs. They are cared for from a sufficient number of adequately qualified staff with appropriate knowledge and professional competence.

In addition, all handling with pigs included in the project, will be carried out according to Directive 2010/63/EU, which updates and replaces the 1986 Directive 86/609/EEC, on the protection of animals used for scientific purposes. The aim of the Directive is to improve the welfare of animals used for experimental purposes, and anchors firmly the principle of the Three Rs, to Replace, Reduce and Refine in the use of animals in experiments. Reduction: Researcher will evaluate the minimal number of animal to obtain significant results for the outcome of the project. Refinement: Care will be taken to respect the legal and good practice standards for housing and handling with pigs. In the majority of cases, pigs will be kept in their regular environment. However all procedures will be conducted minimizing animal distress (e.g. blood sample collection). Replacement is not relevant in TREASURE project, because for the scope of the project i.e. foreseen studies, there is no replacement possible, i.e. other (laboratory) species can’t be used.

To ensure that all above-mentioned European and national regulations will be respected, TREASURE Consortium will appoint an Ethical Committee, composed by 3 members selected among the Partners with relevant qualification according to national and/or EU regulations (Directive 2010/63/EU). The Ethical Committee will report about the observance of rules of animal welfare and protection during the practices foreseen by the project in a specific chapter of the Periodic Progress Reports and the Final Report.

TREASURE Ethical Committee will monitor that ethical standards, EU regulations (Directive 2010/63/EU) and Guidelines of Horizon 2020 will be rigorously applied, regardless of the country in which the research is carried out.

Research protocols involving pigs are not defined in details yet. Most of the research will be done with pigs reared in usual production conditions; in such case Directive 2010/63/EU is not relevant. Animals will not be sacrificed for research purposes. Animals will be slaughtered in commercial or authorized abattoirs following the EU legislation. The potential ethical issues that will be addressed are the following: 1) Collection of blood from live animals. Collection of blood from live animals will be carried out only if necessary i.e. if not possible to substitute it with collection of blood at slaughter in commercial abattoirs. In such case, blood samples for research purposes will be collected by experienced and professional veterinarians, following good veterinary practices and EU legislation (approval of Ethical Commitee and competent national authority will be obtained). 2) Collection of feces from live animals. It is not subjected to ethical protocols if carried out without any direct inspection of the rectum and without any invasive stimulation practices. TREASURE ethical committee will evaluate trial protocols and instruct if feces collection protocols should be submitted to the competent national authority before trials will be conducted. Whenever possible, the collection of feces (intestinal content) after slaughter will substitute the collection of feces on live animals. 3) Feeding trials will be conducted without any distress to the animals changing only the diet components while assuring all needed nutrients to the animals, according to the current available knowledge on diet requirements. TREASURE Ethical committee will evaluate if any ethical approval might be needed according to the national and EU legislation. 4) Immunocastration (vaccination against GnRH) of the pigs is not subject to ethical protocols. The vaccination will be carried out by professional veterinarians who will apply the standard protocols for the use of the approved vaccine Improvac® (http://www.ema.europa.eu/). 5) Castration of the animals. Castration of male piglets is a routine practice in pig breeding allowed in first 7 days post partum (Council Directive 2008/120/EC laying down minimum standards for protection of pigs). TREASURE does not involve partners from CH, N where anaesthesia/analgesia is required; in the case of Serbia, relevant legislation (Sl. glasnik RS 6/2010 and 57/2014) agrees with Council Directive 2008/120/EC. 6) Metabolism cages. Experiments with animals in metabolic cages are foreseen in growing Iberian pigs. In this particular case, the approval has been already obtained by the competent authority (but will also be considered by TREASURE Ethical commitee).

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The partners from EU countries are directly subject to EU legislation regarding ethical standards in research. As regards Serbia (non-EU country); a requirement for ethical committee approval is implemented also in their respective national legislation (Law on animal welfare i.e. Zakon o dobrobiti životinja, Sl. Glasnik RS 41/2009; http://demo.paragraf.rs/WebParagrafDemo/#str_11). In addition, Serbia signed EU convention for the protection of vertebrate animals used for experimental and other scientific purposes (entry in force in 2011). Nonetheless, TREASURE Ethic committee will take care, that the ethical standards and guidelines of Horizon2020 will be rigorously applied, regardless of the country in which the research will be carried out. Concerning that, both Serbian partners have signed the relevant attestation.

In regard to the material imported to EU (e.g. Serbia), if/when relevant, import permits will be obtained from the competent authority in accordance with Regulation 1774/2002/EC which forsees under Article 23 (1) (a), that Member States’ competent authorities may authorise the use of animal by-products of all categories for research purposes (i.e. national rules apply). However, only DNA is foreseen to be sent (extracted with reagents that destroy potential biological contaminants) for the analysis of DNA markers.

None material will be exported from EU to non-EU countries.

Ethics issues table

1. HUMAN EMBRYOS/FOETUSES Page

Does your research involve Human Embryonic Stem Cells (hESCs)? No Will they be directly derived from embryos within this project? No Are they previously established cells lines? No Does your research involve the use of human embryos? No Does your research involve the use of human foetal tissues / cells? No 2. HUMANS Page Does your research involve human participants? No Are they volunteers for experiments in social or human sciences No research? Are they persons unable to give informed consent? No Are they vulnerable individuals or groups? No Are they children/minors? No Are they patients? No Are they healthy volunteers for medical studies? No Does your research involve physical interventions on the study No participants? Does it involve invasive techniques? No Does it involve collection of biological samples? No If your research involves processing of genetic information, please also complete the section “Protection of personal data” [Box 4].

3. HUMAN CELLS / TISSUES Page Does your research involve human cells or tissues? If your No research involves human embryos/foetuses, please also complete the section “Human Embryos/Foetuses” [Box 1]. Are they available commercially? No Are they obtained within this project? No

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3. HUMAN CELLS / TISSUES Page Are they obtained within another project? No Are they deposited in a biobank? No

4. PROTECTION OF PERSONAL DATA Page Does your research involve personal data collection and/or No processing? Does it involve the collection and/or processing of sensitive No personal data (e.g.: health, sexual lifestyle, ethnicity, political opinion, religious or philosophical conviction)? Does it involve processing of genetic information? No Does it involve tracking or observation of participants? No Does your research involve further processing of previously No collected personal data (secondary use)?

5. ANIMALS Page Does your research involve animals? Yes 24-33 Are they vertebrates? Yes 24-33 Are they non-human primates? No Are they genetically modified? No Are they cloned farm animals? No Are they endangered species? No Please indicate the species involved (Max. number of characters 1000): Sus scrofa; (Local pig breeds)

6. NON-EU COUNTRIES Page Does your research involve non-EU countries? Yes 1 Countries:(Maximum number of characters allowed: 1000 Serbia Do you plan to use local resources (e.g. animal and/or human tissue 24-33 samples, genetic material, live animals, human remains, materials of Yes historical value, endangered fauna or flora samples, etc.)? Do you plan to import any material - including personal data - from non-EU countries into the EU? If you consider importing data, please No also complete the section "Protection of Personal Data" [Box 4]. Specify material and countries involved (Maximum number of characters allowed: 1000) Blood samples will be taken during life, tissue samples will be taken post-mortem (after slaughter) following the usual life cycle in farming and standard slaughter procedures Do you plan to export any material - including personal data -from No the EU to non-EU countries? If you consider exporting data, please also complete the section "Protection of Personal Data" [Box 4]. Specify material and countries involved (Maximum number of characters allowed: 1000) If your research involves low and/or lower middle income No countries, are benefits-sharing measures foreseen? Could the situation in the country put the individuals taking part in No

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6. NON-EU COUNTRIES Page the research at risk? 7. ENVIRONMENT PROTECTION Page vi Directive 2001/18/EC - vii Directive 2009/41/EC - viii Regulation EC No 1946/2003 - ix Directive 2008/56/EC x Council Directive 92/43/EEC -xi Council Directive 79/409/EEC - xii Council Regulation EC No 338/97 Does your research involve the use of elements that may cause No harm to the environment, to animals or plants? Does your research deal with endangered fauna and/or flora No and/or protected areas? Does your research involve the use of elements that may cause No harm to humans, including research staff? 8. DUAL USE Page Does your research have the potential for military applications? No 9. MISUSE Page Does your research have the potential for No malevolent/criminal/terrorist abuse? 10. OTHER ETHICS ISSUES Page Are there any other ethics issues that should be taken into No consideration? Please specify Maximum number of characters 1000 I confirm that I have taken into account all ethics issues described above and if any ethics issues apply, I have attached the required documents.

"The Agency reserves the right to verify and will reject any costs charged by a participant related to tasks that the H2020 model Grant Agreement reserves for the project Coordinator."

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CONSORTIUM AGREEMENT

THIS CONSORTIUM AGREEMENT is based upon REGULATION (EU) No 1290/2013 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 11 December 2013 laying down the rules for the participation and dissemination in “Horizon 2020 – the Framework Programme for Research and Innovation (2014-2020)” (hereinafter referred to as “the Rules”), and the European Commission Multi-beneficiary General Model Grant Agreement and its Annexes, and is made on 1/2/2015, hereinafter referred to as the Effective Date between:

1. KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS), 5055431, established in Hacquetova ulica 17, LJUBLJANA 1000, Slovenia, SI23887729, represented for the purposes of signing this Agreement by director, Andrej SIMONČIČ,

“the coordinator” and the following other beneficiaries,

2. UNIVERZA V LJUBLJANI (UL), 5085063, established in KONGRESNI TRG 12, LJUBLJANA 1000, Slovenia, SI54162513,

3. KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM) SI2, 1625403000, established in SMIHELSKA CESTA 14, NOVO MESTO 8000, Slovenia, SI70711798,

4. IFIP-INSTITUT DU PORC ASSOCIATION (IFIP) FR3, 775681323/00051317P, established in RUE LESPAGNOL 5, PARIS 75020, France, FR13775681323,

5. INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA), 180070039, established in Rue De L'Universite 147, PARIS CEDEX 07 75338, France, FR57180070039,

6. Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH), AZ15-1113-1, established in Haller Str. 20, Wolpertshausen 74549, Germany, DE146787977,

7. SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG), 080159438, established in SVETOSIMUNSKA CESTA 25, ZAGREB 10000, Croatia, HR76023745044,

TREASURE 634476 - Consortium Agreement

8. SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFOS) HR6, 030036907, established in KRALJA PETRA SVACICA 1/ D, OSIJEK 31000, Croatia, HR98816779821,

9. AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA (AGRIS), CF02270290907, established in LOCALITA BONASSAI SS 291 KM 18,6, SASSARI 07100, Italy, IT02270290907,

10. ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI (ANAS) IT2, 862427CF0272290584, established in VIA LAZZARO SPALLANZANI 4, ROMA 00161, Italy, IT01028581005,

11. UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI), R.D. n. 2102/1923, established in Piazza San Marco 4, Florence 50121, Italy, IT01279680480,

12. CENTRI REGIONALI PER LE TECNOLOGIEAGROALIMENTARI SCARL (CERTA) SCARL, 251120CF03496570718, established in VIA GRAMSCI 89/91, FOGGIA 71100, Italy, IT03496570718,

13. ALMA MATER STUDIORUM – UNIVERSITA DI BOLOGNA (UNIBO), CF80007010376, established in VIA ZAMBONI 33, BOLOGNA 40126, Italy, IT01131710376,

14. STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA) IT14, 217562/CF00166540344, established in VIALE FAUSTINO TANARA 31/A, PARMA 43100, Italy, IT00166540344,

15. LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS), 302536989 , established in A MICKEVICIAUS GATVE 9, KAUNAS 44307, Lithuania, LT100005579315 ,

16. INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC), established in Praça General Barbosa, Viana do Castelo 4900, Portugal, PT503761877,

TREASURE 634476 - Consortium Agreement

17. UNIVERSIDADE DE EVORA (UEVORA) PCUP, 482/79, established in Largo dos Colegiais 2, Evora 7000-803, Portugal, PT501201920,

18. INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN (IAH), 07017537/538200, established in AUTOPUT 16, BEOGRAD 11080, Serbia, RS100014262,

19. FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG), 07029845, established in Nemanjina 6, BELGRADE 11080, Serbia, RS100198802,

20. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA), established in Carretera de la Coruna Km7.5, MADRID 28040, Spain, ESQ2821013F,

21. AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) ES8, established in CALLE SERRANO 117, MADRID 28006, Spain, ESQ2818002D

22. CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC- IRTA (CREDA) ES3, 2214, established in PARC MEDITERRANI DE LA TECNOLOGIA EDIFICI ESAB C/ESTEVE TERRRADES 8, CASTELLDEFELS 08860, Spain, ESG64068463,

23. CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX), established in AUTOVIA A5 KM 372 FINCA LA ORDEN GUADAJIRA, LOBON BADAJOZ 06187, Spain, ESS0600428G,

24. INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA), established in CTRA C-59 KM 12 TORRE MARIMON, CALDES DE MONTBUI BARCELONA 08140, Spain, ESQ5855049B,

25. ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO (AECERIBER) ES5, 3474/603368, established in CALLE SAN FRANCISCO 51 1 DERECHA, ZAFRA BADAJOZ 06300, Spain, ESG06053334, hereinafter, jointly or individually, referred to as ”Parties” or ”Party”

TREASURE 634476 - Consortium Agreement

relating to the Action entitled

DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS in short

TREASURE hereinafter referred to as “Project” or Action

WHEREAS: The Parties, having considerable experience in the field concerned, have submitted a proposal for the Project to the Funding Authority as part of the Horizon 2020 – the Framework Programme for Research and Innovation (2014-2020) The Parties wish to specify or supplement binding commitments among themselves in addition to the provisions of the specific Grant Agreement to be signed by the Parties and the EC (hereinafter “Grant Agreement”). The Parties agree that this Consortium Agreement concerning the Project supersedes all pre- existing Agreements, Letters of Intent, Memorandums of Understanding, etc. between some or all of the Parties. The Parties are aware that this Consortium Agreement is based upon the DESCA model consortium agreement.

NOW, THEREFORE, IT IS HEREBY AGREED AS FOLLOWS:

Section 1: Definitions

1.1 Definitions Words beginning with a capital letter shall have the meaning defined either herein or in the Rules or in the Grant Agreement including its Annexes.

1.2 Additional Definitions “Consortium Plan” Consortium Plan means the description of the Action and the related agreed budget as first defined in the Grant Agreement and its Annexes which may be updated by the General Assembly.

"Funding Authority" Funding Authority means the body awarding the grant for the Project.

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“Defaulting Party” Defaulting Party means a Party which the General Assembly has identified to be in breach of this Consortium Agreement and/or the Grant Agreement as specified in Section 4.2 of this Consortium Agreement.

“Needed” Needed means:

For the implementation of the Project: Access Rights are Needed if, without the grant of such Access Rights, carrying out the tasks assigned to the recipient Party would be impossible, significantly delayed, or require significant additional financial or human resources.

For the exploitation of own Results: Access Rights are Needed if, without the grant of such Access Rights, the Exploitation of own Results would be technically or legally impossible.

“Legitimate interest/s” Party’s interes/s of any kind that may be claimed in the cases provided for in this Consortium Agreement. To this end the Party must prove that failure to take into account of its interest/s would result in its suffering disproportionately great harm.

“Software”

Software means sequences of instructions to carry out a process in, or convertible into, a form executable by a computer and fixed in any tangible medium of expression.

Section 2: Purpose

The purpose of this Consortium Agreement is to specify with respect to the Project the relationship among the Parties, in particular concerning the organisation of the work between the Parties, the management of the Project and the rights and obligations of the Parties concerning inter alia liability, Access Rights and dispute resolution.

Section 3: Entry into force, duration and termination

3.1 Entry into force An entity becomes a Party to this Consortium Agreement upon signature of this Consortium Agreement by a duly authorised representative. This Consortium Agreement shall have effect from the Effective Date identified at the beginning of this Consortium Agreement.

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An entity becomes a Party to the Consortium Agreement upon signature of the accession document (Attachment 2) by the new Party and the Coordinator. Such accession shall have effect from the date identified in the accession document.

3.2 Duration and termination This Consortium Agreement shall continue in full force and effect until complete fulfilment of all obligations undertaken by the Parties under the Grant Agreement and under this Consortium Agreement.

However, this Consortium Agreement or the participation of one or more Parties to it may be terminated in accordance with the terms of this Consortium Agreement. If the Grant Agreement - is not signed by the Funding Authority or a Party, or - is terminated (according to article 50.1), or - if a Party's participation in the Grant Agreement is terminated, this Consortium Agreement shall automatically terminate in respect of the affected Party/ies, subject to the provisions surviving the expiration or termination under Section 3.3 of this Consortium Agreement and The Parties agree that if a Party requests to terminate its participation in this Consortium Agreement, this request will be considered as a request for termination in the Grant Agreement, according to article 50.2. The provisions of the Grant Agreement and of this Consortium Agreement regarding termination shall apply as hereafter complemented. If a Party wishes to terminate its participation in the Grant Agreement and this Consortium Agreement, it shall send a request in writing to the Coordinator. Such request shall fully set out the reasons for which such withdrawal is deemed necessary. The Coordinator submits the request to the General Assembly, who may require that certain conditions are fulfilled by the withdrawing Party, in the interest of the Project. In case of one Party’s withdrawal, the other Parties shall use reasonable endeavours to reach a timely agreement on how to reallocate the requesting Party's tasks under the Consortium Plan, and their related budget and EC contribution, so that the overall objectives of the Project can still be met after the Party’s withdrawal. Following the decisions above, the Coordinator shall promptly notify the Commission, for its approval and any needed Grant Agreement amendment procedure.

3.3 Survival of rights and obligations The provisions relating to Access Rights and Confidentiality, for the time period mentioned therein, as well as for Liability, Applicable law and Settlement of disputes shall survive the expiration or termination of this Consortium Agreement. Termination shall not affect any rights or obligations of a Party leaving the Consortium incurred prior to the date of termination, unless otherwise agreed between the General Assembly and the leaving Party. This includes the obligation to provide all input, deliverables and documents for the period of its participation.

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Section 4: Responsibilities of Parties

4.1 General principles Each Party undertakes to take part in the efficient implementation of the Project, and to cooperate, perform and fulfil, promptly and on time, all of its obligations under the Grant Agreement and this Consortium Agreement as may be reasonably required from it and in a manner of good faith as prescribed by Belgian law. Each Party undertakes to notify promptly, in accordance with the governance structure of the Project, any significant information, fact, problem or delay likely to affect the Project. Each Party shall promptly provide all information reasonably required by a Consortium Body or by the Coordinator to carry out its tasks. Each Party shall take reasonable measures to ensure the accuracy of any information or materials it supplies to the other Parties.

4.2 Breach Save in the case of Force Majeure, in the event that a responsible Consortium Body identifies a breach by a Party of its obligations under this Consortium Agreement or the Grant Agreement (e.g.: improper implementation of the Project), the Coordinator or, if the Coordinator is in breach of its obligations, the Party appointed by the General Assembly, will give formal notice to such Party requiring that such breach will be remedied within 30 calendar days from the date of receipt of the written notice by the Party. If such breach is substantial and is not remedied within that period or is not capable of remedy, the General Assembly may decide to declare the Party to be a Defaulting Party and to decide on the consequences thereof which may include termination of its participation.

4.3 Involvement of third parties A Party that enters into a subcontract or otherwise involves third parties (including but not limited to Linked Third Parties) in the Project remains responsible for carrying out its relevant part of the Project and for such third party’s compliance with the provisions of this Consortium Agreement and of the Grant Agreement. It has to ensure that the involvement of third parties does not affect the rights and obligations of the other Parties under this Consortium Agreement and the Grant Agreement.

4.4. Granting and revoking user rights in the Participant Portal To enable the access to documents and necessary transactions for the Project at the Participant Portal, each Party can assign the user rights to any specifically identified person, provided that appropriate confidentiality obligations are in place, according to its needs. To avoid unauthorised access to information and unauthorised transactions for a Project, each Party shall immediately revoke user rights to a person leaving the Project or said Party. In case a Party terminates the participation in the Project, the Coordinator shall immediately revoke user rights to all contact persons of that Party.

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Section 5: Liability towards each other

5.1 No warranties In respect of any information or materials (incl. Results and Background) supplied by one Party to another Party, or to another Party's Linked Third Party under the Project, no warranty or representation of any kind is made, given or implied as to the sufficiency or fitness for purpose nor as to the absence of any infringement of any proprietary rights of third parties. Therefore, - the recipient Party shall in all cases be entirely and solely liable for the use to which it puts such information and materials, and - no Party granting Access Rights shall be liable in case of infringement of proprietary rights of a third party resulting from any other Party (or its Linked Third Party) exercising its Access Rights.

5.2 Limitations of contractual liability No Party shall be responsible to any other Party for any indirect or consequential loss or similar damage such as, but not limited to, loss of profit, loss of revenue or loss of contracts, provided such damage was not caused by a wilful act or by a breach of confidentiality. A Party’s aggregate liability towards the other Parties collectively shall be limited to once the Party’s share of the total costs of the Project as identified in Annex 2 of the Grant Agreement provided such damage was not caused by a wilful act or gross negligence. The terms of this Consortium Agreement shall not be construed to amend or limit any Party’s statutory or non-contractual liability.

5.3 Damage caused to third parties Each Party shall be solely liable for any loss, damage or injury to third parties resulting from the performance of the said Party’s obligations by it or on its behalf under this Consortium Agreement or from its use of Results or Background.

5.4 Force Majeure No Party shall be considered to be in breach of this Consortium Agreement if it is prevented from fulfilling its obligations under the Consortium Agreement by Force Majeure. Each Party will notify the competent Consortium Bodies of any Force Majeure without undue delay. If the consequences of Force Majeure for the Project are not overcome within 6 weeks after such notification, the transfer of tasks - if any - shall be decided by the competent Consortium Bodies.

Section 6: Governance structure

6.1 General structure The organisational structure of the Consortium shall comprise the following Consortium Bodies: General Assembly is the decision-making body of the Consortium.

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Coordinator is the legal entity acting as the intermediary between the Parties and the Funding Authority. The Coordinator shall, in addition to its responsibilities as a Party, perform the tasks assigned to it as described in the Grant Agreement and this Consortium Agreement. Management Support Team (also referred to as Management Office) assists the General Assembly and the Coordinator. Work Package (WP) Leaders are responsible for the progress of their WP with regard to the overall work plan and shall report to the Coordinator and General Assembly. Scientific Committee is the advisory body which is responsible for the scientific progress of work and for the appropriate exploitation and dissemination of results. It is composed of WP leaders. Ethical Committee is the advisory body which will be responsible that ethical standards and guidelines of Horizon2020 will be rigorously applied, regardless of the country in which the research is carried out. Members will be appointed by the General Assembly at kick-off meeting. Quality Control Committee is the advisory body which will perform the internal control of the project progress, in particular to identify tasks or partners at risk and propose corrective measures. Members will be appointed by the General Assembly at kick-off meeting.

6.2 Members The General Assembly shall consist of one representative of each Party (hereinafter referred to as “Member”) whose initial names and contact details are listed in Attachment 5. Each Member shall be deemed to be duly authorised to deliberate, negotiate and decide on all matters listed in Section 6.3.6 of this Consortium Agreement. The Coordinator shall chair all meetings of the General Assembly, unless decided otherwise by the General Assembly. The Parties agree to abide by all decisions of the General Assembly. This does not prevent the Parties from submitting a dispute for resolution in accordance with the provisions of settlement of disputes in Section 11.8 of this Consortium Agreement.

6.3 Operational procedures for the General Assembly

6.3.1 Representation in meetings Any Member: - should be present or represented at any meeting; - may appoint a substitute by presenting a proxy statement to the chairperson of the General Assembly, in order to attend the meeting and vote; such substitute shall be bound by the non- disclosure obligations such as described in section 10 of this Consortium Agreement; - shall participate in a cooperative manner in the meetings.

6.3.2 Preparation and organisation of meetings 6.3.2.1 Convening meetings: The chairperson shall convene ordinary meetings of the General Assembly at least once a year and shall also convene extraordinary meetings at any time upon written request of any Member. 6.3.2.2 Notice of a meeting:

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The chairperson shall give notice in writing (via email is admitted, but with acknowledgement of receipt) of a meeting to each Member as soon as possible and no later than 14 calendar days preceding an ordinary meeting and 7 calendar days preceding an extraordinary meeting. 6.3.2.3 Sending the agenda: The chairperson shall send each Member a written original agenda no later than 14 calendar days preceding the ordinary meeting, or 7 calendar days before an extraordinary meeting. 6.3.2.4 Adding agenda items: Any agenda item requiring a decision by the Members must be identified as such on the agenda. Any Member may add an item to the original agenda by written notification to all of the other Members no later than 7 calendar days preceding the ordinary and 5 days preceding an extraordinary meeting. 6.3.2.5 During a meeting of the General Assembly the Members present or represented can unanimously agree to add a new item to the original agenda. 6.3.2.6 Any decision may also be taken without a meeting if the chairperson circulates to all Members a written document which is then signed by the defined majority of Members (see Section 6.3.3 of this Consortium Agreement). Such document shall include the deadline for responses. 6.3.2.7 Meetings of the General Assembly may also be held by teleconference or other telecommunication means. 6.3.2.8 Decisions will only be binding once the relevant part of the minutes has been accepted according to Section 6.3.5 of this Consortium Agreement.

6.3.3 Voting rules and quorum 6.3.3.1 The General Assembly shall not deliberate and decide validly unless two-thirds (2/3) of its Members are present or represented (quorum). If the quorum is not reached, the chairperson shall convene extraordinary meeting within 15 calendar days. If in this meeting the quorum is not reached once more, the General Assembly shall be entitled to decide even if less than quorum of Members are present or represented. 6.3.3.2 Each Member shall have one vote. 6.3.3.3 Defaulting Parties may not vote, nor shall their presence account for the necessary quorum. The Coordinator may not vote on decisions regarding a proposal to the Comission for a change of Coordinator. 6.3.3.4 Decisions shall be taken by a majority of two-thirds (2/3) of the votes cast. Decisions listed in Article 6.3.6 (iii) shall be taken by the unanimity of votes. 6.3.3.5 The present and represented Members may decline to participate in a vote of the General Assembly by stating that they abstain, in which case they shall not be counted for the purposes of determining the majority of the votes as described in Section 6.2.3.4. 6.3.3.6 When a decision has been adopted or rejected, it may be reconsidered and proposed in another further meeting of the General Assembly only if the request comes at the initiative of at least two-thirds (2/3) of the Members

6.3.4 Veto rights 6.3.4.1 A Member which can show that its own work, time for performance, costs, liabilities,

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intellectual property rights or other legitimate interests would be severely affected by a decision of the General Assembly may exercise a veto with respect to the corresponding decision or relevant part of the decision. 6.3.4.2 When the decision is foreseen on the original agenda, a Member may veto such a decision during the meeting only. 6.3.4.3 When a decision has been taken on a new item added to the agenda before or during the meeting, a Member may veto such decision during the meeting and within 15 days after the draft minutes of the meeting are sent. The exercise of the veto shall be supported by a written justification by the Party exercising such veto: - within 10 calendar days after the meeting at which the veto is exercised or - within 10 calendar days after the draft minutes are sent In both cases the written justification will be made available to all Parties. 6.3.4.4 In case of exercise of veto, the Members shall make every effort to resolve the matter which occasioned the veto to the general satisfaction of all Members. 6.3.4.5 A Party may not veto decisions relating to its identification as a Defaulting Party. The Defaulting Party may not veto decisions relating to its participation and termination in the consortium or the consequences of them. 6.3.4.6 A Party requesting to leave the consortium may not veto decisions relating thereto.

6.3.5 Minutes of meetings 6.3.5.1 The chairperson shall produce written minutes of each meeting which shall be the formal record of all decisions taken. He shall send draft minutes to all Members within 10 calendar days of the meeting. 6.3.5.2 The minutes shall be considered as accepted if, within 15 calendar days from sending, no Member has sent an objection in writing to the chairperson with respect to the accuracy of the draft of the minutes. 6.3.5.3 The chairperson shall send the accepted minutes to all the Members of the General Assembly, and to the Coordinator, who shall safeguard them. If requested the Coordinator shall provide authenticated duplicates to Parties.

6.3.6 Decisions of the General Assembly The General Assembly shall be free to act on its own initiative to formulate proposals and take decisions in accordance with the procedures set out herein. The following decisions shall be taken by the General Assembly:

(i) Content, finances and intellectual property rights - Proposals for changes to Annexes 1 and 2 of the Grant Agreement to be agreed by the Funding Authority - Changes to the Consortium Plan - Modifications to Attachment 1 (Background Included) - Additions to Attachment 3 (List of Third Parties for simplified transfer according to Section 8.2.2) - Proposals for amicable settlement of disputes

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(ii) Evolution of the consortium - Entry of a new Party to the consortium and approval of the settlement on the conditions of the accession of such a new Party - Withdrawal of a Party from the consortium and the approval of the settlement on the conditions of the withdrawal - Identification of a breach by a Party of its obligations under this Consortium Agreement or the Grant Agreement - Declaration of a Party to be a Defaulting Party - Remedies to be performed by a Defaulting Party - Termination of a Defaulting Party’s participation in the consortium and measures relating thereto

(iii) Decision to be taken by unanimity - Proposal to the Funding Authority for suspension of all or part of the project - Proposal to the Funding Authority for termination of the Project and the Consortium Agreement - Proposal to the Funding Authority for a change of the Coordinator

(iv) Appointments General Assembly shall agree on the Members of the Ethical Committee, Quality Control Committee and Management Support Team, upon the proposal by the Coordinator.]

In the case of abolished tasks as a result of a decision of the General Assembly, Members shall rearrange the tasks of the Parties concerned. Such rearrangement shall take into consideration the legitimate commitments taken prior to the decisions, which cannot be cancelled.

6.4 Coordinator

6.4.1 The Coordinator shall be the intermediary between the Parties and the Funding Authority and shall perform all tasks assigned to it as described in the Grant Agreement and in this Consortium Agreement.

6.4.2 In particular, the Coordinator shall be responsible for: - monitoring compliance by the Parties with their obligations - keeping the address list of Members and other contact persons updated and available - collecting, reviewing and submitting information on the progress of the Project and reports and other deliverables (including financial statements and related certification) to the Funding Authority - preparing the meetings, proposing decisions and preparing the agenda of General Assembly meetings, chairing the meetings, preparing the minutes of the meetings and monitoring the implementation of decisions taken at meetings - transmitting promptly documents and information connected with the Project, - administering the financial contribution of the Funding Authority and fulfilling the financial tasks described in Section 7.3

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- providing, upon request, the Parties with official copies or originals of documents which are in the sole possession of the Coordinator when such copies or originals are necessary for the Parties to present claims. If one or more of the Parties is late in submission of any project deliverable, the Coordinator may nevertheless submit the other parties’ project deliverables and all other documents required by the Grant Agreement to the Funding Authority in time. 6.4.3 If the Coordinator fails in its coordination tasks, the General Assembly may propose to the Funding Authority to change the Coordinator. 6.4.4 The Coordinator shall not be entitled to act or to make legally binding declarations on behalf of any other Party or of the consortium, unless explicitly stated otherwise in the Grant Agreement or this Consortium Agreement. 6.4.5 The Coordinator shall not enlarge its role beyond the tasks specified in this Consortium Agreement and in the Grant Agreement.

6.5 Management Support Team (Management Office) The Management Support Team shall be proposed by the Coordinator. It shall be appointed by the General Assembly and shall assist and facilitate the work of the General Assembly. The Management Support Team shall provide assistance to the Coordinator for executing the decisions of the General Assembly. It shall be responsible for the day-to-day management of the Project.

Section 7: Financial provisions

7.1 General Principles

7.1.1 Distribution of Financial Contribution The financial contribution of the Funding Authority to the Project shall be distributed by the Coordinator according to: - the Consortium Plan - the approval of reports by the Funding Authority, and - the provisions of payment in Section 7.3.

A Party shall be funded only for its tasks carried out in accordance with the Consortium Plan.

7.1.2 Justifying Costs In accordance with its own usual accounting and management principles and practices, each Party shall be solely responsible for justifying its costs with respect to the Project towards the Funding Authority. Neither the Coordinator nor any of the other Parties shall be in any way liable or responsible for such justification of costs towards the Funding Authority.

7.1.3 Funding Principles

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A Party which spends less than its allocated share of the budget as set out in the Consortium Plan or – in case of reimbursement via unit costs - implements less units than foreseen in the Consortium Plan will be funded in accordance with its actual duly justified eligible costs only. A Party that spends more than its allocated share of the budget as set out in the Consortium Plan will be funded only in respect of duly justified eligible costs up to an amount not exceeding that share.

7.1.4 Financial Consequences of the termination of the participation of a Party A Party leaving the consortium shall refund all payments it has received except the amount of contribution accepted by the Funding Authority or another contributor. Furthermore a Defaulting Party shall, within the limits specified in Section 5.2 of this Consortium Agreement, bear any reasonable and justifiable additional costs occurring to the other Parties in order to perform its and their tasks.

7.2 Budgeting The budget set out in the Consortium Plan shall be valued in accordance with the usual accounting and management principles and practices of the respective Parties.

7.3 Payments 7.3.1 Payments to Parties are the exclusive tasks of the Coordinator. In particular, the Coordinator shall: - notify the Party concerned promptly of the date and composition of the amount transferred to its bank account, giving the relevant references - perform diligently its tasks in the proper administration of any funds and in maintaining financial accounts - undertake to keep the Community financial contribution to the Project separated from its normal business accounts, its own assets and property, except if the Coordinator is a Public Body or is not entitled to do so due to statutory legislation. - With reference to Articles 21.2 and 21.3.2 of the Grant Agreement, no Party shall before the end of the Project receive more than its allocated share of the maximum grant amount from which the amounts retained by the Funding Authority for the Guarantee Fund and for the final payment have been deducted. 7.3.2 The payment schedule, which contains the transfer of pre-financing and interim payments to Parties, will be handled according to the following: - Funding of costs included in the Consortium Plan will be paid to Parties after receipt from the Funding Authority without undue delay and in conformity with the provisions of the Grant Agreement. Costs accepted by the Funding Authority will be paid to the Party concerned. - The Coordinator is entitled to withhold any payments due to a Party identified by a responsible Consortium Body to be in breach of its obligations under this Consortium Agreement or the Grant Agreement or to a Beneficiary which has not yet signed this Consortium Agreement.

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- The Coordinator is entitled to recover any payments already paid to a Defaulting Party. The Coordinator is equally entitled to withhold payments to a Party when this is suggested by or agreed with the Funding Authority.

Section 8: Results

8.0 Ownership of Results Results are owned by the Party that generates them.

8.1 Joint ownership of Results

Two or more beneficiaries own results jointly if they have jointly generated them, and it is not possible to establish the respective contribution of each Beneficiary or separate them for the purpose of applying for, obtaining or maintaining their protection. In case of joint ownership, joint owners shall agree, in writing, on the allocation and terms of exercise of their joint ownership through joint ownership agreement. Unless otherwise agreed: - each of the joint owners shall be entitled to use their jointly owned Results for non-commercial research or educational activities on a royalty-free basis, and without requiring the prior consent of the other joint owner(s), and - each of the joint owners shall be entitled to otherwise Exploit the jointly owned Results and to grant non-exclusive licenses to third parties (without any right to sub-license), if the other joint owners are given: (a) at least 45 calendar days advance notice; and (b) fair and reasonable compensation. Notwithstanding letter a), in case sub-licenses are granted to third parties for commercial purposes, the prior written consent of the joint owners shall be obtained.

8.2 Transfer of Result/s 8.2.1 Each Party may transfer ownership of its own Result/s and of its own share of jointly owned Result/s following the procedures of the Grant Agreement Article 30. 8.2.2 Each Party may identify specific third parties it intends to transfer the ownership of its Result/s to in Attachment (3) to this Consortium Agreement. The other Parties hereby waive their right to object to a transfer to listed third parties according to the Grant Agreement Article 30.1. 8.2.3 The transferring Party shall, however, at the time of the transfer, inform the other Parties of such transfer and shall ensure that the rights of the other Parties will not be affected by such transfer. Any addition to Attachment (3) after signature of this Agreement requires a decision of the General Assembly. 8.2.4 The Parties recognize that in the framework of a merger or an acquisition of an important part of its assets, it may be impossible under applicable EU and national laws on mergers and acquisitions for a Party to give the full 45 calendar days prior notice for the transfer as foreseen

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in the Grant Agreement. In this case, notice of transfer will be given as soon as possible but no later than 30 (thirty) days after the transfer.

8.2.5 The obligations above apply only for as long as other Parties still have - or still may request - Access Rights to the Results.

8.3 Dissemination

8.3.1 Dissemination of own Results 8.3.1.1 During the Project and for a period of 1 year after the end of the Project, the dissemination of own Results by one or several Parties including but not restricted to publications and presentations, shall be governed by the procedure of Article 29.1 of the Grant Agreement subject to the following provisions. Prior notice of any planned publication shall be given, including copy of the proposed publication, to the other Parties at least 30 calendar days before the publication. Any objection to the planned publication shall be made in accordance with the Grant Agreement in writing to the Coordinator and to the Party or Parties proposing the dissemination within 15 calendar days after receipt of the notice. If no objection is made within the time limit stated above, the publication is permitted. Notwithstanding the generality of the foregoing, for short written or oral communications on conferences, seminars, workshops, meetings a prior notice of at least 15 calendar days and 7 calendar days for objection, shall apply. 8.3.1.2 An objection is justified if (a) the protection of the objecting Party's Results or Background would be adversely affected (b) the objecting Party's legitimate academic or commercial interests in relation to the Results or Background would be significantly harmed. The objection has to include a precise request for necessary modifications. An objection is not considered as justified if the publication or communication concerns operating recommendations of products, combined or not, or results (e.g. data) indicating a risk for health or environment. 8.3.1.3 If an objection has been raised the involved Parties shall discuss how to overcome the justified grounds for the objection on a timely basis (for example by amendment to the planned publication and/or by protecting information before publication) and the objecting Party shall not unreasonably continue the opposition if appropriate measures are taken following the discussion. The objecting Party can request a publication delay of not more than 90 calendar days from the time it raises such an objection. After 90 calendar days the publication is permitted, provided that Confidential Information of the objecting Party has been removed from the Publication as indicated by the objecting Party.

8.3.2 Dissemination of another Party’s unpublished Results or Background A Party shall not include in any dissemination activity another Party's Results or Background without obtaining the owning Party's prior written approval, unless they are already published.

8.3.3 Cooperation obligations The Parties undertake to cooperate to allow the timely submission, examination, publication and

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defence of any dissertation or thesis for a degree which includes their Results or Background subject to the confidentiality and publication provisions agreed in this Consortium Agreement.

8.3.4 Use of names, logos or trademarks 8.3.4.1 of the Parties Nothing in this Consortium Agreement shall be construed as conferring rights to use in advertising, publicity or otherwise the name of the Parties or any of their logos or trademarks without their prior written approval. 8.3.4.2 of the Project If one or several logos that include the name of the Project are created or if a Party files a trademark that includes the name of the Project, each Party shall be entitled to use any logo or trademark related to the project royalty free and on non exclusive basis for the execution of the Project only, even if the logo or trademark has been filed by a single Party only. The Parties shall agree on further use of the logo of the project and its possible needed protection measures in a specific agreement.

8.3.5 Dissemination and communication policy The Parties undertake to promote the action and disseminate its results respecting the guidelines and rules established by the General Assembly and Grant Agreement. In particular, according to Article 29.2 of the Grant Agreement, each Party shall ensure open access to all peer-reviewed publications: - deposit the publication (at the latest on publication) in a repository for scientific publications; - ensure open access to this publication at the latest within six months of publication (and the publishing Party should aim to give access to the research data needed to validate the Results published).

Section 9: Access Rights

9.1 Background included

9.1.1 In Attachment 1, the Parties have identified and agreed on the Background for the Project and have also, where relevant, informed each other that Access to specific Background is subject to legal restrictions or limits. Anything not identified in Attachment 1 shall not be the object of Access Right obligations regarding Background. 9.1.2 Any Party can propose to the General Assembly to modify its Background in Attachment 1.

9.2 General Principles

9.2.1 Each Party shall implement its tasks in accordance with the Consortium Plan and shall bear sole responsibility for ensuring that its acts within the Project do not knowingly infringe third party property rights.

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9.2.2 Any Access Rights granted expressly exclude any rights to sublicense unless expressly agreed otherwise in writing by all Parties concerned. 9.2.3 Access Rights shall be free of any administrative transfer costs. 9.2.4 Access Rights are granted on a non-exclusive basis. 9.2.5 Results and Background shall be used only for the purposes for which Access Rights to it have been granted. 9.2.6 All requests for Access Rights shall be made in writing. The granting of Access Rights may be made conditional on the acceptance of specific conditions aimed at ensuring that these rights will be used only for the intended purpose(s), only for as long as it is necessary for those purposes, and that appropriate confidentiality obligations are in place. 9.2.7 The requesting Party must show that the Access Rights are Needed.

9.3 Access Rights for implementation Access Rights to Results and Background Needed for the performance of the own work of a Party under the Project shall be granted on a royalty-free basis, unless otherwise agreed for Background in Attachment 1.

9.4 Access Rights for Exploitation 9.4.1 Access Rights to Results if Needed for Exploitation of a Party's own Results shall be granted on fair and reasonable conditions. Access rights to Results for internal non-commercial research activities and for non-commercial educational purposes shall be granted on an royalty-free-basis. 9.4.2 Access Rights to Background if Needed for Exploitation of a Party's own Results, including for research on behalf of a third party, shall be granted on Fair and Reasonable conditions. 9.4.3 A request for Access Rights may be made up to 12 (twelve) months after the end of the Project or, in the case of Section 9.7.2.1.2, after the termination of the requesting Party’s participation in the Project.

9.5 Access Rights for Linked Third Parties (Affiliated Entities and Third party with a legal link to a beneficiary) Linked Third Parties have Access Rights under the conditions of the Grant Agreement Articles 25.4 and 31.4., if they are identified in Attachment 4 to this Consortium Agreement]. Such Access Rights must be requested by the Linked Third Party from the Party that holds the Background or Results. Access Rights to Linked Third Parties shall be granted on Fair and Reasonable conditions and upon written bilateral agreement. Linked Third Parties which obtain Access Rights in return fulfil all confidentiality and other obligations accepted by the Parties under the Grant Agreement or this Consortium Agreement as if such Linked Third Parties were Parties. Access Rights may be refused to Linked Third Parties if such granting is contrary to the legitimate interests of the Party which owns the Background or the Results. Access Rights granted to any Linked Third Party are subject to the continuation of the Access Rights of the Party to which it is affiliated, and shall automatically terminate upon termination of the Access Rights granted to such Party.

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Upon cessation of the status of Linked Third Party, any Access Rights granted to such Linked Third Party shall lapse. Further arrangements with Linked Third Parties may be negotiated in separate agreements.

9.6 Additional Access Rights For the avoidance of doubt any grant of Access Rights not covered by the Grant Agreement or this Consortium Agreement shall be at the absolute discretion of the owning Party and subject to such terms and conditions as may be agreed between the owning and receiving Parties.

9.7 Access Rights for Parties entering or leaving the consortium 9.7.1 New Parties entering the consortium As regards Results developed before the accession of the new Party, the new Party will be granted Access Rights on the conditions applying for Access Rights to Background. 9.7.2 Parties leaving the consortium 9.7.2.1 Access Rights granted to a leaving Party 9.7.2.1.1 Defaulting Party Access Rights granted to a Defaulting Party and such Party's right to request Access Rights shall cease immediately upon receipt by the Defaulting Party of the formal notice of the decision of the General Assembly to terminate its participation in the consortium.

9.7.2.1.2 Non-defaulting Party A non-defaulting Party leaving voluntarily and with the other Parties' consent shall have Access Rights to the Results developed until the date of the termination of its participation. It may request Access Rights within the period of time specified in Section 9.4.3. 9.7.2.2 Access Rights to be granted by any leaving Party Any Party leaving the Project shall continue to grant Access Rights pursuant to the Grant Agreement and this Consortium Agreement as if it had remained a Party for the whole duration of the Project.

9.8 Specific Provisions for Access Rights to Software For the avoidance of doubt, the general provisions for Access Rights provided for in this Section 9 are applicable also to Software. Parties’ Access Rights to Software do not include any right to receive source code or object code ported to a certain hardware platform or any right to receive respective Software documentation in any particular form or detail, but only as available from the Party granting the Access Rights.

9.9 Specific Provisions for Material Transfer Parties agree that the transfer of material for the performance of the Project shall be implemented by a Material transfer Agreement as set out in Attachment 7.

9.10 Specific Provisions for Access right to databases generated in the Project Consortium has opted to take part in the pilot study on Open Research Data which implies an obligation to prepare Data Management Plan as a distinct deliverable.

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Parties agree that the Scientific Committee is responsible to prepare data management plan(s) in agreement with Horizon 2020 rules and guidelines throughout the duration of the Project. The proposals on data management by the Scientific Committee will be voted at the General Assembly.

Section 10: Non-disclosure of information

10.1 All information in whatever form or mode of communication, which is disclosed by a Party (the “Disclosing Party”) to any other Party (the “Recipient”) in connection with the Project during and for its implementation which has been explicitly marked as “confidential” at the time of disclosure, or when disclosed orally has been identified as confidential at the time of disclosure and has been confirmed and designated in writing within 15 calendar days from oral disclosure at the latest as confidential information by the Disclosing Party, is “Confidential Information”.

10.2 The Recipients hereby undertake in addition and without prejudice to any commitment of non-disclosure under the Grant Agreement, for a period of 4 years after the end of the Project: - not to use Confidential Information otherwise than for the purpose for which it was disclosed; - not to disclose Confidential Information to any third party without the prior written consent by the Disclosing Party; - to ensure that distribution of Confidential Information internally within their organisation shall take place on a strict need-to-know basis; and - to return to the Disclosing Party on demand all Confidential Information which has been supplied to or acquired by the Recipients including all copies thereof and to delete all information stored in a machine readable form. The Recipients may keep a copy to the extent it is required to keep, archive or store such Confidential Information because of compliance with applicable laws and regulations or for the proof of on-going obligations.

10.3 The Recipients shall be responsible for the fulfilment of the above obligations on the part of their employees or third parties involved in the Project and shall ensure that they remain so obliged, as far as legally possible, during and after the end of the Project and/or after the termination of the contractual relationship with the employee or third party.

10.4 The above shall not apply for disclosure or use of Confidential Information, if and in so far as the Recipient can show that: - the Confidential Information becomes publicly available by means other than a breach of the Recipient’s confidentiality obligations; - the Disclosing Party subsequently informs the Recipient that the Confidential Information is no longer confidential; - the Confidential Information is communicated to the Recipient without any obligation of confidence by a third party who is to the best knowledge of the Recipient in lawful possession thereof and under no obligation of confidence to the Disclosing Party;

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- the disclosure or communication of the Confidential Information is foreseen by provisions of the Grant Agreement; - the Confidential Information, at any time, was developed by the Recipient completely independently of any such disclosure by the Disclosing Party; or - the Confidential Information was already known to the Recipient prior to disclosure or - the Recipient is required to disclose the Confidential Information in order to comply with applicable laws or regulations or with a court or administrative order, subject to the provision Section 10.7 hereunder.

10.5 The Recipient shall apply the same degree of care with regard to the Confidential Information disclosed within the scope of the Project as with its own confidential and/or proprietary information, but in no case less than reasonable care.

10.6 Each Party shall promptly advise the other Party in writing of any unauthorised disclosure, misappropriation or misuse of Confidential Information after it becomes aware of such unauthorised disclosure, misappropriation or misuse.

10.7 If any Party becomes aware that it will be required, or is likely to be required, to disclose Confidential Information in order to comply with applicable laws or regulations or with a court or administrative order, it shall, to the extent it is lawfully able to do so, prior to any such disclosure - notify the Disclosing Party of said request, and - comply with the Disclosing Party’s reasonable instructions to protect the confidentiality of the information.

Section 11: Miscellaneous

11.1 Attachments, inconsistencies and severability This Consortium Agreement consists of this core text and Attachment 1 (Background included) Attachment 2 (Accession document) Attachment 3 (List of Third Parties for simplified transfer according to Section 8.2.2) Attachment 4 (Identified Linked Third Parties) Attachment 5 (List of initial members of Consortium bodies and other contact persons) Attachment 6 (List of the Party's bank account details) Attachment 7 (Material Transfer Agreement) In case the terms of this Consortium Agreement are in conflict with the terms of the Grant Agreement, the terms of the latter shall prevail. In case of conflicts between the attachments and the core text of this Consortium Agreement, the latter shall prevail. Should any provision of this Consortium Agreement become invalid, illegal or unenforceable, it shall not affect the validity of the remaining provisions of this Consortium Agreement. In such a case, the Parties concerned shall be entitled to request that a valid and practicable provision be negotiated which fulfils the purpose of the original provision.

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11.2 No representation, partnership or agency Except as otherwise provided in Section 6.4.4, no Party shall be entitled to act or to make legally binding declarations on behalf of any other Party or of the Consortium. Nothing in this Consortium Agreement shall be deemed to constitute a joint venture, agency, partnership, interest grouping or any other kind of formal business grouping or entity between the Parties.

11.3 Notices and other communication Any notice to be given under this Consortium Agreement shall be in writing to the addresses and recipients as listed in the most current address list kept by the Coordinator based on the initial list of members and other contact persons in Attachment 5.

Formal notices: If it is required in this Consortium Agreement (Sections 4.2, 9.7.2.1.1, and 11.4) that a formal notice, consent or approval shall be given, such notice shall be signed by an authorised representative of a Party and shall either be served personally or sent by mail with recorded delivery or telefax with receipt acknowledgement. Other communication: Other communication between the Parties may also be effected by other means such as e-mail with acknowledgement of receipt, which fulfils the conditions of written form. Any change of persons or contact details shall be notified immediately by the respective Party to the Coordinator. The address list shall be accessible to all concerned. Any change to the bank account details shall be immediately notified by the respective Party to the Coordinator.

11.4 Assignment and amendments Except as set out in Section 8.2, no rights or obligations of the Parties arising from this Consortium Agreement may be assigned or transferred, in whole or in part, to any third party without the other Parties’ prior formal approval. Amendments and modifications to the text of this Consortium Agreement not explicitly listed in Section 6.3.6 require a separate written agreement to be signed between all Parties.

11.5 Mandatory national law Nothing in this Consortium Agreement shall be deemed to require a Party to breach any mandatory statutory law under which the Party is operating.

11.6 Language This Consortium Agreement is drawn up in English, which language shall govern all documents, notices, meetings, arbitral proceedings and processes relative thereto. Any translation shall be for convenience only and shall have no legal effects.

11.7 Applicable law This Consortium Agreement shall be construed in accordance with and governed by the laws of Belgium excluding its conflict of law provisions.

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11.8 Settlement of disputes The Parties shall endeavour to settle amicably any dispute controversy or claim arising under, out of or relating to this contract and any subsequent amendments of this contract, including without limitation, its formation, validity, binding effect, interpretation, performance, breach of termination, as well as non-contractual claims. If and to the extent that, any such dispute, controversy or claims has not been settled amicably within 90 calendar days of the commencement, the courts of Brussels shall have exclusive jurisdiction.

Section 12: Signatures

AS WITNESS:

The Parties have caused this Consortium Agreement to be duly signed by the undersigned authorised representatives in 25 separate signature pages the day and year first above written. The Coordinator shall send a copy of each original signature page, duly executed, to each Party together with the text of this Consortium Agreement within 60 days from the receipt of the last original signature page.

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1. KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS)

Signature

Name Andrej SIMONČIČ

Title Director

Date

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2. UNIVERZA V LJUBLJANI (UL)

Signature

Name Ivan SVETLIK

Title Rector

Date

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3. KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM)

Signature

Name Jože SIMONČIČ

Title Director

Date

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4. IFIP-INSTITUT DU PORC ASSOCIATION (IFIP)

Signature

Name Bernard FOSTIER

Title Director

Date

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5. INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)

Signature

Name Patrick HERPIN

Title President of the INRA Research Center Rennes

Date

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6. Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH)

Signature

Name Rudolf BUEHLER

Title Chairman

Date

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7. SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG)

Signature

Name Tajana KRIČKA

Title Dean

Date

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8. SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFOS)

Signature

Name Vlado GUBERAC

Title Dean

Date

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9. AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA (AGRIS)

Signature

Name Raffaele CHERCHI

Title Director

Date

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10. ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI (ANAS)

Signature

Name Andrea CRISTINI

Title President

Date

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11. UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI)

Signature

Name Paolo NANNIPIERI

Title Director of Department

Date

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12. CENTRI REGIONALI PER LE TECNOLOGIEAGROALIMENTARI SCARL (CERTA)

Signature

Name Loreto GESUALDO

Title President

Date

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13. ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA (UNIBO)

Signature

Name Andrea SEGRÈ

Title Director of Department

Date

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14. STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA)

Signature

Name Andrea ZANLARI

Title President

Date

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15. LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS)

Signature

Name Vaiva LESAUSKAITE

Title Vice-Rector for Research

Date

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16. INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC)

Signature

Name Carlos RODRIGUES

Title Director

Date

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17. UNIVERSIDADE DE EVORA (UEVORA) PCUP

Signature

Name Paulo QUARESMA

Title Vice rector

Date

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18. INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN (IAH)

Signature

Name Čedomir RADOVIĆ

Title Vice-director

Date

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19. FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG)

Signature

Name Milica PETROVIĆ

Title Dean

Date

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20. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA)

Signature

Name Manuel LAINEZ ANDRÉS

Title Director

Date

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21. AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)

Signature

Name Alicia CASTRO

Title Vice President for Scientific and Technical Research

Date

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22. CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI- UPC-IRTA (CREDA)

Signature

Name José M. GIL

Title Director

Date

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23. CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX)

Signature

Name Germán PUEBLA OVANDO

Title Director

Date

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24. INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA)

Signature

Name Jordi DE LA CUESTA FERNANDEZ

Title Chief Financial Officer

Date

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25. ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO (AECERIBER)

Signature

Name Elena DIÉGUEZ GARBAYO

Title Secretary

Date

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[Attachment 1: Background included] According to the Grant Agreement (Article 24) Background is defined as “data, know-how and/or information whatever their form or nature, tangible or intangible, including any rights such as intellectual property rights which are (i) held by participants prior to their accession to the Action (ii) identified by the participants in accordance with article 42 of Horizon 2020 Rules for Participation (Regulation (EU) No 1290/2013 and (iii) needed for carrying out the Action or for exploiting the results of the Action. Because of this need, Access Rights have to be granted in principle, but parties must identify and agree amongst them on the Background for the project. This is the purpose of this attachment.

PARTY No.1 As to KMETIJSKI INSTITUT SLOVENIJE -AGRICULTURAL INSTITUTE OF SLOVENIA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.2 As to UNIVERZA V LJUBLJANI, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

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PARTY No.3 As to KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.4 As to IFIP-INSTITUT DU PORC ASSOCIATION, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits.

Software tools and database applications can’t be shared due to restricted user licences. Each Party involved in carcass and meat quality database development should have its own license for software; if, after the end of the Action, Party prefers to host their data at IFIP hosting and maintenance costs will be charged. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

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PARTY No.5 As to INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. Background: Results pertaining to the EU-FP6 project Q-Porkchains (2007-2012) and other studies on biomarkers of technological and sensory quality traits of pork (associations between gene expression and quality trait levels), data regarding combinations of biomarkers of pork quality as predictors of meat quality grades. Pre-existing molecular and computational biology approaches for porcine gut metagenomics developed at INRA, from faeces sampling and microbiota DNA extraction to sequencing and bioinformatics analyses of the datasets. Specific limitations and/or conditions for implementation (Article 25.2 Grant Agreement): Molecular tools (biomarkers) as well as mathematical equations for evaluation or prediction of meat quality have been obtained in programs co-financed by other French partners, which share intellectual property rights on these data. Specific limitations and/or conditions for exploitation (Article 25.3 Grant Agreement): Mathematical equations for evaluation or prediction of meat quality may be subject to use under licences agreement for exploitation. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.6 As to Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V., it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

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PARTY No.7 As to SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.8 As to SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.9 As to AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits,

TREASURE 634476 - Consortium Agreement

- to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.10 As to ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.11 As to UNIVERSITA DEGLI STUDI DI FIRENZE, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

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PARTY No.12 As to CENTRI REGIONALI PER LE TECNOLOGIEAGROALIMENTARI SCARL, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.13 As to ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA, it is agreed between the parties that, to the best of their knowledge, Option 1: The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. Genotyping data on Italian local and commercial pig breeds and populations obtained from previous projects and useful for activities included in WP1. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.14 As to STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits,

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- to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. E.g. the infrastructure (know-how and facilities) for processing meat into products which shall be Needed by another Party to implement the tasks under the Action. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.15 As to LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.16 As to INSTITUTO POLITECNICO DE VIANA DE CASTELO, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

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PARTY No.17 As to UNIVERSIDADE DE EVORA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.18 As to INSTITUT ZA STOCARSTVO BEOGRAD ZEMUN, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.19 As to FACULTY OF AGRICULTURE – UNIVERSITY OF BELGRADE, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits,

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PARTY No.20 As to INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. Background: Pre-existing molecular biology approaches for genotyping of porcine candidate genes developed at INIA. Genotyping and sequencing data on Iberian pig populations obtained from previous projects and useful for activities included in WP1. Software tools can’t be shared due to restricted user licences. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.21 As to AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

TREASURE 634476 - Consortium Agreement

PARTY No.22 As to CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARIUPC- IRTA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.23 As to CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

PARTY No.24 As to INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits,

TREASURE 634476 - Consortium Agreement

PARTY No.25 As to ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO, it is agreed between the parties that, to the best of their knowledge, The following background is hereby identified and agreed upon for the Project. Specific limitations and/or conditions, shall be as mentioned hereunder: - to implement their own tasks under the Action, Access Rights to the Background which shall be Needed by another Party shall be given on a royalty-free basis, unless it is subject to legal restrictions or limits, - to exploit their own results, Access Rights to the Background which shall be Needed by another Party shall be given under fair and reasonable conditions, unless it is subject to legal restrictions or limits. In such cases an acknowledgement should be provided in any communication or dissemination relevant to the received Background. This represents the status at the time of signature of this Consortium Agreement.

TREASURE 634476 - Consortium Agreement

[Attachment 2: Accession document]

Accession of a new Party to

TREASURE 634476 Consortium Agreement

[official name of the new Party as identified in the Grant Agreement]

Herby consents to become a Party to the Consortium Agreement identified above and accepts all the rights and obligations of a Party starting [date]

KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS), 5055431, established in Hacquetova ulica 17, LJUBLJANA 1000, Slovenia, SI23887729, represented by director, Andrej SIMONČIČ,

Hereby certifies that the Consortium has accepted in the meeting held on [date] the accession of the [official name of the new Party as identified in the Grant Agreement] to the Consortium starting [date].

This accession document has been done in two (2) originals to be duly signed by the undersigned authorised representatives.

[Date and place]

[Name of new Party] Signature Name Title

TREASURE 634476 - Consortium Agreement

[Attachment 3: List of Third Parties for simplified transfer according to Section 8.2.2.]

DRUSTVO REJCEV KRSKOPOLJSKIH PRASICEV ZEMLJISNA ZAJEDNICA PLEMENITA OPCINA TUROPOLJSKA POLJOPRIVREDNO-USLUZNA ZADRUGA LETA CONSORZIO DI TUTELA DELLA CINTA SENESE LIETUVOS NYKSTANCIU UKINIU GYVUNU AUGINTOJU ASOCIACIJA ASSOCIACAO NACIONAL DOS CRIADORES DO PORCO ALENTEJANO ASSOCIACAO NACIONAL DE CRIADORES DE SUINOS DE RACA BISARA EKO SELO CONSORTIUM DU NOIR DE BIGORRE

TREASURE 634476 - Consortium Agreement

[Attachment 4: Identified Linked Third Parties according to section 9.5]

Linked third party to beneficiary Short Beneficiary Short name name DRUSTVO REJCEV KRSKOPOLJSKIH DKP 3. KMETIJSKO GOZDARSKA KGZS-NM PRASICEV ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO Bäuerliche Erzeugergemeinschaft BESH AG 4. Bäuerliche BESH Schwäbisch Hall AG Erzeugergemeinschaft Schwäbisch Hall w. V. ZEMLJISNA ZAJEDNICA PLEMENITA POT 7. SVEUCILISTE U ZAGREBU UNIZG OPCINA TUROPOLJSKA AGRONOMSKI FAKULTET POLJOPRIVREDNO-USLUZNA LETA 8. SVEUCILISTE JOSIPA JURJA PFOS ZADRUGA LETA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU UNIVERSITA DEGLI STUDI DI UNISS 9. AGRIS SARDEGNA - AGRIS SASSARI AGENZIA PER LA RICERCA IN AGRICOLTURA CONSORZIO DI TUTELA DELLA CONCS 11. UNIVERSITA DEGLI STUDI UNIFI CINTA SENESE DI FIRENZE LIETUVOS NYKSTANCIU UKINIU LEFABA 15. LIETUVOS SVEIKATOS LUHS GYVUNU AUGINTOJU ASOCIACIJA MOKSLU UNIVERSITETAS ASSOCIACAO NACIONAL DE ANCSUB 16. INSTITUTO POLITECNICO IPVC CRIADORES DE SUINOS DE RACA DE VIANA DE CASTELO BISARA ASSOCIACAO NACIONAL DOS ANCPA 17. UNIVERSIDADE DE EVORA UEVORA CRIADORES DO PORCO ALENTEJANO EKO SELO DUD 18. INSTITUT ZA IAH STOCARSTVO BEOGRAD- ZEMUN Inštitut za higijenu i tehnologiju mesa IMHT 19. FACULTY OF UNIBG društveno preduzeće AGRICULTURE - UNIVERSITY OF BELGRADE UNIVERSIDAD DE EXTREMADURA UNIEX 20. INSTITUTO NACIONAL DE INIA INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA

TREASURE 634476 - Consortium Agreement

Attachment 5: List of members of Consortium Bodies and other contact persons

Members of the General Assembly

Name Email Party

Marjeta [email protected] 1 KMETIJSKI INSTITUT SLOVENIJE - ČANDEK AGRICULTURAL INSTITUTE OF POTOKAR SLOVENIA (KIS), Hacquetova ulica 17, SI-1000 Ljubljana

Peter DOVČ [email protected] 2 UNIVERZA V LJUBLJANI (UL), BIOTECHNICAL FACULTY, Jamnikarjeva 101, SI-1000 Ljubljana

Andrej [email protected] 3 KMETIJSKO GOZDARSKA KASTELIC ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM), Šmihelska cesta 14, SI-8000 Novo mesto

Marie-José [email protected] 4 IFIP-INSTITUT DU PORC MERCAT ASSOCIATION (IFIP), La Motte au Vicomte, B.P. 35104, 35651 Le Rheu Cedex, France

Benedicte [email protected] 5 INSTITUT NATIONAL DE LA LEBRET [email protected] RECHERCHE AGRONOMIQUE (INRA), UMR1348 PEGASE, F- 35590 Saint-Gilles

Christoph [email protected] 6 Bäuerliche Erzeugergemeinschaft ZIMMER Schwäbisch Hall w. V. (BESH), Haller Str. 20, Wolpertshausen 74549, Germany

TREASURE 634476 - Consortium Agreement

Name Email Party

Danijel [email protected] 7 SVEUCILISTE U ZAGREBU KAROLYI AGRONOMSKI FAKULTET (UNIZG), Svetosimunska cesta 25, Zagreb, HR-10000

Goran [email protected] 8 SVEUCILISTE JOSIPA JURJA KUSEC STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFO, Kralja Petra Svacica 1/D, HR-31000 Osijek

Sebastiano [email protected] 9 AGRIS SARDEGNA - AGENZIA PER PORCU LA RICERCA IN AGRICOLTURA (AGRIS), Localita Bonassai SS 291 km 18,6, IT-07100 Sassari

Maurizio [email protected] 10 ASSOCIAZIONE NAZIONALE GALLO ALLEVATORI SUINI (ANAS), Via Lazzaro Spallanzani 4, IT- 00161Roma

Carolina [email protected] 11 UNIVERSITA DEGLI STUDI DI PUGLIESE FIRENZE (UNIFI), Via delle Cascine 5, IT-50144 Firenze

Loreto [email protected] 12 CENTRI REGIONALI PER LE GESUALDO TECNOLOGIE AGROALIMENTARI SCARL (CERTA), Via Gramsci 89/91, IT-71100 Foggia

Luca [email protected] 13 ALMA MATER STUDIORUM - FONTANESI UNIVERSITA DI BOLOGNA (UNIBO), Viale Fanin 46, IT-40127 Bologna

TREASURE 634476 - Consortium Agreement

Name Email Party

Carlo [email protected] 14 STAZIONE SPERIMENTALE PER DIAFERIA L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA), Viale Faustino Tanara 31/A, IT-43100Parma

15 LIETUVOS SVEIKATOS MOKSLU Violeta [email protected] UNIVERSITETAS (LUHS), R. Zebenkos RAZMAITE [email protected] 12, LT-82317 Baisogala, Radviliskis distr.

José Pedro [email protected] 16 INSTITUTO POLITECNICO DE ARAÚJO VIANA DE CASTELO (IPVC), Praça General Barbosa, PT-4900 , Viana do Castelo

17 UNIVERSIDADE DE EVORA Rui (UEVORA), Dpt Medicina [email protected] CHARNECA Veterinaria, Apartado 94, PT- 7002-554 Evora

Čedomir [email protected] 18 INSTITUT ZA STOCARSTVO RADOVIĆ BEOGRAD-ZEMUN (IAH), Autoput 16, RS-11080 Beograd

Radomir [email protected] 19 FACULTY OF AGRICULTURE - SAVIĆ UNIVERSITY OF BELGRADE (UNIBG), Nemanjina 6 , RS-11080, Beograd

Ana Isabel [email protected] 20 INSTITUTO NACIONAL DE FERNÁNDEZ INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA), Dpt. de Mejora Genetica Animal. Ctra. de la Coruña, Km. 7.5, ES- 28040 Madrid

TREASURE 634476 - Consortium Agreement

Name Email Party

Rosa Maria [email protected] 21 AGENCIA ESTATAL CONSEJO NIETO SUPERIOR DE INVESTIGACIONES LIÑÁN CIENTIFICAS (CSIC), Estacion Zaidin, C/Profesor Albareda s/n, ES- 18008 Granada

Jose M. GIL [email protected] 22 CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC-IRTA (CREDA) Parc Mediterrani de la Tecnologia Edifici ESAB C/Esteve Terrrades 8, ES-08860, CASTELLDEFELS

Mercedes [email protected] 23 23. CENTRO DE INVESTIGACIONES IZQUIERDO CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX), Autovia A5 KM 372 Finca La Orden Guadajira , ES-06187 Lobon Badajoz

Maria [email protected] 24 INSTITUT DE RECERCA I Angels TECNOLOGIA OLIVER AGROALIMENTARIES (IRTA), Finca Camps i Arm, ES-17121 Monells

Elena [email protected] 25 ASOCIACION ESPANOLA DE DIÉGUEZ CRIADORES DE CERDO IBERICO GARBAYO (AECERIBER), Calle San Francisco 51 1 Derecha , ES-06300 , Zafra Badajoz

TREASURE 634476 - Consortium Agreement

List of contacts

1-KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS) Role Name Email Address Primary Coordinator Marjeta meta.candek- Hacquetova ulica 17, Contact ČANDEK [email protected] SI-1000 Ljubljana POTOKAR* LEAR, Coordinator Tine ŠTEBE [email protected] Hacquetova ulica 17, Contact SI-1000 Ljubljana Project Financial Meta ČUFAR [email protected] Hacquetova ulica 17, Signatory SI-1000 Ljubljana Project Specific Legal Andrej [email protected] Hacquetova ulica 17, Authorised Signatory SIMONČIČ SI-1000 Ljubljana Coordinator Contact Martin ŠKRLEP [email protected] Hacquetova ulica 17, SI-1000 Ljubljana Team Member Nina BATOREK [email protected] Hacquetova ulica 17, LUKAČ SI-1000 Ljubljana Team Member Urška TOMAŽIN [email protected] Hacquetova ulica 17, SI-1000 Ljubljana *General Assembly Member

2-UNIVERZA V LJUBLJANI (UL) Role Name Email Address Participant Peter DOVČ* [email protected] BF, Jamnikarjeva 101, SI- Contact 1000 Ljubljana LEAR Staška MRAK [email protected] Kongresni trg 12, SI-1000 Participant JAMNIK Ljubljana Contact Project Specific Ivan SVETLIK [email protected] Kongresni trg 12, SI-1000 Legal Authorised Ljubljana Signatory *General Assembly Member

TREASURE 634476 - Consortium Agreement

3-KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM) GA Role Name Email Address YES Participant Andrej [email protected] Šmihelska cesta 14, Contact KASTELIC* SI-8000 Novo mesto LEAR NO Participant Zdenka [email protected] Šmihelska cesta 14, Contact KRAMAR SI-8000 Novo mesto NO Project Specific Jože [email protected] Šmihelska cesta 14, Legal Authorised SIMONČIČ SI-8000 Novo mesto Signatory NO Project Financial Barbara [email protected] Šmihelska cesta 14, Signatory DERGANC SI-8000 Novo mesto *General Assembly Member

4. IFIP-INSTITUT DU PORC ASSOCIATION (IFIP) Role Name Email Address Participant Marie-José [email protected] La Motte au Vicomte, Contact MERCAT* B.P. 35104, F-35651 Le Rheu Cedex Participant Valerie [email protected] 5 rue l’Espagnol, F- Contact HEBERT 75020 PARIS Cedex LEAR, Project Bernard [email protected] 5 rue l’Espagnol, F- Specific Legal FOSTIER 75020 PARIS Cedex Authorised Signatory, Project Financial Signatory *General Assembly Member

TREASURE 634476 - Consortium Agreement

5. INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) Role Name Email Address Participant Benedicte [email protected] UMR1348 PEGASE Contact LEBRET* [email protected] F-35590 Saint-Gilles LEAR Virginie [email protected] CHAOY Project Specific Patrick [email protected] Legal Authorised HERPIN Signatory Project Financial Edwige [email protected] Signatory LASSALAS Team Member Juliette [email protected] UMR1388 GenPhySE, RIQUET 24 chemin de Borde- Rouge 31326 Auzeville, France Team Member Jordi [email protected] UMR1313 GABI, ESTELLÉ Domaine de Vilvert, FR-78530 Jouy-en- Josas Team Member Florence florence.garcia- UMR1348 PEGASE F- GARCIA- [email protected] 35590 Saint-Gilles LAUNAY Team Member Diane [email protected] UAR1209 PlaGe, 24 ESQUERRE chemin de Borde- Rouge, F- 31326 Auzeville Team Member Ludovic [email protected] UMR 1348 PEGASE, BROSSARD 35590 Saint-Gilles, France Team Member Justine [email protected] UMR 1348 PEGASE, FAURE 35590 Saint-Gilles, France Team Member Bertrand [email protected] UMR1388 GenPhySE, SERVIN 24 chemin de Borde- Rouge 31326 Auzeville, France *General Assembly Member

TREASURE 634476 - Consortium Agreement

6. Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH) Role Name Email Address Participant Christoph [email protected] Haller Str. 20, Contact ZIMMER* Wolpertshausen 74549, Germany LEAR Christiane [email protected]; Haller Str. 20, Participant CLAUS [email protected] Wolpertshausen Contact 74549, Germany Participant Rudolf BUEHLER [email protected] Haller Str. 20, Contact Wolpertshausen 74549, Germany Team Mathias PETIG [email protected] Haller Str. 20, Member Wolpertshausen 74549, Germany *General Assembly Member

7. SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG) Role Name Email Address Participant Svetosimunska cesta 25, Danijel KAROLYI* [email protected] Contact Zagreb, HR-10000 LEAR, [email protected] Svetosimunska cesta 25, Participant Ivona FILIPOVIĆ [email protected] Zagreb, HR-10000 Contact Project Specific Legal Authorised Svetosimunska cesta 25, Signatory Tajana KRIČKA [email protected] Zagreb, HR-10000 Project Financial Signatory Participant Svetosimunska cesta 25, Marija CERJAK [email protected] Contact Zagreb, HR-10000 Svetosimunska cesta 25, Team Member Zoran LUKOVIĆ [email protected] Zagreb, HR-10000 Svetosimunska cesta 25, Team Member Krešimir SALAJPAL [email protected] Zagreb, HR-10000 *General Assembly Member

TREASURE 634476 - Consortium Agreement

8. SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFOS) Role Name Email Address Participant Contact Goran [email protected] Kralja Petra Svacica 1/D, KUSEC* HR-31000 Osijek LEAR Sonja MARIĆ [email protected] Kralja Petra Svacica 1/D, HR-31000 Osijek Project Specific Legal Vlado [email protected] Kralja Petra Svacica 1/D, Authorised Signatory GUBERAC HR-31000 Osijek Project Financial Signatory Participant Contact Vladimir [email protected] Kralja Petra Svacica 1/D, MARGETA HR-31000 Osijek Team Member Boris LUKIC [email protected] Kralja Petra Svacica 1/D, HR-31000 Osijek Team Member Kristina [email protected] Kralja Petra Svacica 1/D, BUDIMIR HR-31000 Osijek Participant Contact Ivona [email protected] Kralja Petra Svacica 1/D, DJURKIN HR-31000 Osijek *General Assembly Member

9. AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA (AGRIS) Role Name Email Address Participant Sebastiano [email protected] Localita Bonassai SS Contact PORCU* 291 km 18,6, IT- 07100 Sassari LEAR Antonio PIRISI [email protected] Localita Bonassai SS Participant 291 km 18,6, IT- Contact 07100 Sassari Project Specific Raffaele CHERCHI [email protected] Viale Adua n. 2/c, IT- Legal Authorised 07100 Sassari Signatory Project Financial Signatory Team member Roberta [email protected] Localita Bonassai SS COMUNIAN 291 km 18,6, IT- 07100 Sassari *General Assembly Member

TREASURE 634476 - Consortium Agreement

10. ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI (ANAS) Role Name Email Address Participant Contact Maurizio [email protected] Via Lazzaro LEAR GALLO* Spallanzani 4, IT- 00161Roma Project Specific Legal Andrea CRISTINI [email protected] Via Lazzaro Authorised Signatory Spallanzani 4, IT- Project Financial 00161Roma Signatory *General Assembly Member

11. UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI) Role Name Email Address Participant Carolina [email protected] Via delle Cascine 5, Contact PUGLIESE* IT-50144 Firenze LEAR Marigrazia [email protected] c/o NIC - Nuovo CATANIA Ingresso Careggi, Padiglione 3 - Largo Brambilla, 3, IT-50134 - Firenze Participant Riccardo BOZZI [email protected] Via delle Cascine 5, Contact IT-50144 Firenze Team member Doria BENVENUTI [email protected] Via delle Cascine 5, IT-50144 Firenze Team member Silvano LANCINI [email protected] Via delle Cascine 5, IT-50144 Firenze Project Paolo NANNIPIERI [email protected] Piazza San Marco 4, Specific Legal IT-50121Florence Authorised Signatory Project Financial Signatory *General Assembly Member

TREASURE 634476 - Consortium Agreement

12. CENTRI REGIONALI PER LE TECNOLOGIE AGROALIMENTARI SCARL (CERTA) Role Name Email Address Loreto GESUALDO* [email protected] Via Gramsci 89/91, IT-71122 Foggia Participant Giuseppe DALFINO [email protected] Via Gramsci 89/91, Contact IT-71122 Foggia LEAR Participant Carmela COSOLA [email protected] Via Gramsci 89/91, Contact IT-71122 Foggia Project Specific Loreto GESUALDO [email protected] Via Gramsci 89/91, Legal Authorised IT-71122 Foggia Signatory *General Assembly Member

13. ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA (UNIBO) Role Name Email Address Participant Luca FONTANESI* [email protected] Viale Fanin 46, IT- Contact 40127 Bologna LEAR Chiara BASALTI [email protected] Project Specific Andrea SEGRÈ distal.dipartimento.respammini Via Zamboni 33, Legal Authorised [email protected] IT-40126 Bologna Signatory Project Financial Signatory Team Member Leonardo NANNI [email protected] Viale Fanin 46, IT- COSTA 40127 Bologna Participant Simone [email protected] Viale Fanin 46, IT- Contact MACCAFERRI 40127 Bologna Participant Sabrina GINEPRI [email protected] Viale Fanin 46, IT- Contact 40127 Bologna Participant Nadia GRANDINI [email protected] Viale Fanin 46, IT- Contact 40127 Bologna Team Member Stefania DALLOLIO [email protected] Viale Fanin 46, IT- 40127 Bologna *General Assembly Member

TREASURE 634476 - Consortium Agreement

14. STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA) Role Name Email Address Participant Carlo DIAFERIA* [email protected] Viale Faustino Tanara Contact 31/A, IT-43100Parma LEAR Monica ERMES [email protected] Viale Faustino Tanara Project Financial 31/A, IT-43100Parma Signatory Project Specific Andrea ZANLARI [email protected] Viale Faustino Tanara Legal Authorised 31/A, IT-43100Parma Signatory Team Member Roberta VIRGILI [email protected] Viale Faustino Tanara 31/A, IT-43100Parma *General Assembly Member

15. LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS) Role Name Email Address R. Zebenkos 12, LT- Participant Violeta [email protected] 82317 Baisogala, Contact RAZMAITE* [email protected] Radviliskis distr. Ingrida A Mickeviciaus Gatve 9, LEAR [email protected] ULOZIENE LT-44307Kaunas Project Specific Vaiva A Mickeviciaus Gatve 9, Legal Authorised [email protected] LESAUSKAITE LT-44307Kaunas Signatory R. Zebenkos 12, LT- Participant Ruta [email protected] 82317 Baisogala, Contact ŠVEISTIENE Radviliskis distr. R. Zebenkos 12, LT- Violeta Team member [email protected] 82317 Baisogala, JUSKIENE Radviliskis distr *General Assembly Member

TREASURE 634476 - Consortium Agreement

16. INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC) Role Name Email Address Participant Contact José Pedro [email protected] Praça General Barbosa ARAÚJO* , PT-4900, Viana do Castelo LEAR Hugo [email protected] Largo 9 de Abril | Ap. DELGADO 186, PT- 4901-911 Viana do Castelo Project Specific Legal Carlos [email protected] Praça General Authorised Signatory RODRIGUES Barbosa, PT-4900 , Project Financial Viana do Castelo Signatory Team member Preciosa PIRES [email protected] Avenida do Atlantico , PT-4900-348, Viana do Castelo Team member Joaquim [email protected] Refoios do Lima, PT- Orlando LIMA 4900-706 Ponte de CERQUEIRA Lima Team member Eliana Pamela [email protected] Refoios do Lima , PT- BARBOSA 4900-706 Ponte de Lima *General Assembly Member

17. UNIVERSIDADE DE EVORA (UEVORA) Role Name Email Address Apartado 94, PT- Participant Contact Rui CHARNECA* [email protected] 7002-554 Evora Project Financial Rui GONÇALVES Largo dos Colegiais 2, [email protected] Signatory PINGO PT-7000-803 Evora LEAR Largo dos Colegiais 2, Paulo QUARESMA [email protected] Project Specific Legal PT-7000-803 Evora Authorised Signatory José TIRAPICOS Apartado 94, PT- Participant Contact [email protected] NUNES 7002-554 Evora Amadeu BORGES Apartado 94, PT - Team member [email protected] FREITAS 7002-554 Évora Miguel SANTOS Apartado 94, PT - Team member [email protected] ELIAS 7002-554 Évora José Manuel Apartado 94, PT - Team member [email protected] MARTINS 7002-554 Évora Apartado 94, PT - Team member José Alberto NEVES [email protected] 7002-554 Évora *General Assembly Member

TREASURE 634476 - Consortium Agreement

18. INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN (IAH) GA Role Name Email Address YES Participant Čedomir [email protected] Autoput 16, Contact RADOVIĆ* RS-11080 Beograd Project Specific Legal Authorised Signatory Project Financial Signatory NO LEAR Nikola [email protected] Autoput 16, Participant STANIŠIĆ RS-11080 Beograd Contact NO Participant Miloš LUKIĆ [email protected] Autoput 16, Contact RS-11080 Beograd NO Participant Marija [email protected] Autoput 16, Contact GOGIĆ RS-11080 Beograd *General Assembly Member

19. FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG) Role Name Email Address Participant Contact Radomir SAVIĆ* [email protected] Nemanjina 6 , RS-11080, Beograd LEAR Katarina [email protected] Nemanjina 6 , SMAKIĆ RS-11080, Beograd Project Specific Legal Milica PETROVIĆ [email protected] Nemanjina 6 , Authorised Signatory RS-11080, Beograd Participant Contact Project Financial Todor VULIĆ [email protected] Nemanjina 6 , Signatory RS-11080, Beograd Team Member Dragan [email protected] Nemanjina 6 , RADOJKOVIĆ RS-11080, Beograd *General Assembly Member

TREASURE 634476 - Consortium Agreement

20. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA) Role Name Email Address Participant Ana Isabel [email protected] Dpt. de Mejora Contact FERNÁNDEZ* Genetica Animal. Ctra. de la Coruña, Km. 7.5, ES-28040 Madrid Participant Juan María [email protected] Centro nacional de I+D Contact GARCÍA del cerdo iberico. CASCO Ctra. de los Santos-Ex 101, km 4.7, apartado 60, ES-06300 Zafra, Badajoz. LEAR Rocio [email protected] Relaciones LANSAC internacionales. Ctra. de la Coruña, Km. 7,5, ES-28040 Madrid Project Financial Arantzazu [email protected] Secretaria General. Signatory CABELLO s Ctra. de la Coruña, Km. LOPEZ 7,5, ES-28040 Madrid Participant Mercedes [email protected] SGIT. Contact MARTINEZ Ctra. de la Coruña, Km. 7,5, ES-28040 Madrid Participant Cristina [email protected] Dpt. de Mejora Contact OVILO Genetica Animal. Ctra. de la Coruña, Km. 7.5, ES-28040 Madrid Project Specific Manuel [email protected] Direction. Legal Authorised LAINEZ Ctra. De la Coruña, Km. Signatory ANDRÉS 7.5, ES-28040 Madrid *General Assembly Member

TREASURE 634476 - Consortium Agreement

21. AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) Role Name Email Address Participant Rosa Maria [email protected] C/Profesor Contact NIETO Albareda s/n, ES- LIÑÁN* 18008 Granada LEAR Ana Maria DE [email protected] Serrano 117, ES- LA FUENTE 28006 Madrid Participant Programas [email protected] Serrano 117, ES- Contact EUROPEOS 28006 Madrid Project Specific Alicia [email protected] Serrano 117, ES- Legal Authorised CASTRO 28006 Madrid Signatory Project Financial Antonio Javier [email protected] Serrano 117, ES- Signatory SANCHEZ- 28006 Madrid HERENCIA Participant Gerencia EEZ [email protected] C/Profesor Contact Albareda s/n, ES- 18008 Granada Participant Ignacio [email protected] C/Profesor Contact FERNANDEZ- Albareda s/n, ES- FIGARES 18008 Granada Team member Isabel [email protected] C/Profesor SEIQUER Albareda s/n, ES- 18008 Granada Team member Manuel [email protected] C/Profesor LACHICA Albareda s/n, ES- 18008 Granada Team member Luis LARA [email protected] C/Profesor Albareda s/n, ES- 18008 Granada *General Assembly Member

TREASURE 634476 - Consortium Agreement

22. CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC-IRTA (CREDA) Role Name Email Address Participant Contact Jose M. GIL* [email protected] Parc Mediterrani de la Tecnologia LEAR Edifici ESAB C/Esteve Terrrades 8, Project Specific Legal ES-08860, Castelldefels Authorised Signatory Project Financial Signatory Team Member Zein KALLAS [email protected] Parc Mediterrani de la Tecnologia Edifici ESAB C/Esteve Terrrades 8, ES-08860, Castelldefels *General Assembly Member

23. CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX) Role Name Email Address Participant Mercedes mercedes.izquierdo@ Autovia A5 KM 372 Finca Contact IZQUIERDO* gobex.es La Orden Guadajira , ES- 06187 Lobon Badajoz LEAR Francisco francisco.hinojalj@go Autovia A5 KM 372 Finca HINOJAL bex.es La Orden Guadajira , ES JUAN Project Specific Germán director.cicytex@gob Autovia A5 KM 372 Finca Legal Authorised PUEBLA ex.es La Orden Guadajira , ES Signatory OVANDO Project Financial Signatory Team Member Javier MATIAS javier.matias@gobex. Autovia A5 KM 372 Finca es La Orden Guadajira , ES Team Member Francisco francisco.hernandez@ Autovia A5 KM 372 Finca HERNÁNDEZ gobex.es La Orden Guadajira , ES *General Assembly Member

TREASURE 634476 - Consortium Agreement

24. INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA) Role Name Email Address Participant Maria Angels [email protected] Finca Camps i Armet Contact OLIVER* ES-17121 Monells LEAR Silvia [email protected] Ctra C-59 KM 12 FERNANDEZ Torre Marimon , ES- LOPEZ 08140, Caldes De Montbui Barcelona Project Specific Jordi DE LA [email protected] Ctra C-59 KM 12 Legal Authorised CUESTA Torre Marimon , ES- Signatory FERNANDEZ 08140, Caldes De Montbui Barcelona Participant Marta GIL [email protected] Finca Camps i Armet Contact ES-17121 Monells Participant Lluis SALVA- [email protected] Finca Camps i Armet Contact VILA ES-17121 Monells Participant Cristina [email protected] Finca Camps i Armet Contact GONZALEZ ES-17121 Monells *General Assembly Member

25. ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO (AECERIBER) Role Name Email Address Participant Elena DIÉGUEZ [email protected] Calle San Francisco 51 Contact GARBAYO 1 Derecha , ES-06300 LEAR , Zafra Badajoz Project Specific Legal Authorised Signatory Project Financial Signatory Team Member Ana SÁNCHEZ [email protected] Calle San Francisco 51 PALOP 1 Derecha , ES-06300, Zafra Badajoz *General Assembly Member

TREASURE 634476 - Consortium Agreement

Attachment 6: List of the Party's bank accounts

1. KMETIJSKI INSTITUT SLOVENIJE - AGRICULTURAL INSTITUTE OF SLOVENIA (KIS) Name of bank: BANKA SLOVENIJE Address of branch: 35, Slovenska, 1000 Ljubljana, Slovenia Full name of the account Kmetijski institut Slovenije – Agricultural Institute of Slovenia holder: Full account number 01100 6030348219 (including bank codes): SWIFT/BIC code BSLJSI2X IBAN code: SI56011006030348219

2. UNIVERZA V LJUBLJANI (UL) Name of bank: BANKA SLOVENIJE

Address of branch: 35, Slovenska, 1000 Ljubljana, Slovenia Full name of the account Univerza v Ljubljani holder: Full account number 01100 6000022236 (including bank codes): Reference BF-TREASURE

SWIFT/BIC code BSLJSI2X

IBAN code: SI5601100 6000022236

3. KMETIJSKO GOZDARSKA ZBORNICA SLOVENIJE, KMETIJSKO GOZDARSKI ZAVOD NOVO MESTO (KGZS-NM) Name of bank: BANKA SLOVENIJE

Address of branch: 35, Slovenska, 1000 Ljubljana, Slovenia Full name of the account Kmetijsko gozdarska zbornica Slovenije, Kmetijsko gozdarski zavod holder: Novo mesto Full account number 01100 6030235311 (including bank codes): SWIFT/BIC code BSLJSI2X

IBAN code: SI56011006030235311

TREASURE 634476 - Consortium Agreement

4. IFIP-INSTITUT DU PORC ASSOCIATION (IFIP) Name of bank: Crédit Agricole de Paris et d'Ile-de-France

Address of branch: CAF Paris EST, 20 Rue de Hector Malot, 75012 Paris, France Full name of the account IFIP – INSTITUT DE PORC holder: Full account number 18206002800022041100114 (including bank codes): SWIFT/BIC code AGRIFRPP882

IBAN code: FR7618206002800022041100114

5. INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) Name of bank: Trésor Public DRFIP Bretagne et Ille et Vilaine, Cité administrative, Avenue Janvier, Address of branch: BP 72102, 35021 Rennes Cedex 9, France Full name of the account INRA, Agent Comptable Secondaire, Domaine de la Motte, BP29, holder: 35650 Le Rheu, France Full account number 10071 35000 00001004696 15 (including bank codes): SWIFT/BIC code TRPUFRP1

IBAN code: FR76 1007 1350 0000 0010 0469 615

6. Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. (BESH) Name of bank: Südwestbank

Address of branch: Im Haal 8, D- 74523 Schwäbisch Hall Full name of the account Bäuerliche Erzeugergemeinschaft Schwäbisch Hall w. V. holder: Full account number 600907000604365004 (including bank codes): SWIFT/BIC code SWBSDESS

IBAN code: DE31600907000604365004

TREASURE 634476 - Consortium Agreement

7. SVEUCILISTE U ZAGREBU AGRONOMSKI FAKULTET (UNIZG) Name of bank: Zagrebačka banka d.d.

Address of branch: Trg bana Josipa Jelačića 10, 10000 Zagreb, Croatia Full name of the account Sveučilište u Zagrebu, Agronomski Fakultet holder: Full account number 2360000-1101221840 (including bank codes): SWIFT/BIC code ZABAHR2X

IBAN code: HR1823600001101221840

8. SVEUCILISTE JOSIPA JURJA STROSSMAYERA U OSIJEKU POLJOPRIVREDNI FAKULTET U OSIJEKU (PFOS) Name of bank: HYPO ALPE-ADRIA-BANK d.d.

Address of branch: Slavonska avenija 6, 10000 Zagreb, Croatia Full name of the account POLJOPRIVREDNI FAKULTET U OSIJEKU holder: Full account number 25000091102019470 (including bank codes): SWIFT/BIC code HAABHR22

IBAN code: HR1725000091102019470

9. AGRIS SARDEGNA - AGENZIA PER LA RICERCA IN AGRICOLTURA (AGRIS) Name of bank: UNICREDIT SPA

Address of branch: Via Budapest, 20, 07100 Sassari, Italy Full name of the account AGRIS Sardegna holder: Full account number 0200817204000102726902 (including bank codes): SWIFT/BIC code UNCRITMM

IBAN code: IT26W0200817204000102726902

TREASURE 634476 - Consortium Agreement

10. ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI (ANAS) Name of bank: BANCA PROSSIMA S.P.A.

Address of branch: Piazza Paolo Ferrari, 10, Filiale di Milano – 20121 Milano, Italy Full name of the account ASSOCIAZIONE NAZIONALE ALLEVATORI SUINI holder: Full account number 0335901600100000060612 (including bank codes): SWIFT/BIC code BCITITMX

IBAN code: IT23K0335901600100000060612

11. UNIVERSITA DEGLI STUDI DI FIRENZE (UNIFI) Name of bank: Unicredit Banca di Roma S.p.A.

Address of branch: Via dei Vecchietti, 11, 50123 Firenze, Italy Full name of the account Università degli Studi di Firenze holder: Full account number 0200802837000041126939 (including bank codes): SWIFT/BIC code UNCRITM1F86

IBAN code: IT88A0200802837000041126939

12. CENTRI REGIONALI PER LE TECNOLOGIEAGROALIMENTARI SCARL (CERTA) Name of bank: BANCA MONTE DEI PASCHI DI SIENA S.P.A.

Address of branch: Corso Giuseppe Garibaldi n. 6 - 71121 Foggia (FG) - Italy Full name of the account CENTRI REGIONALI PER LE TECNOLOGIE holder: Full account number 0103015700 000001213251 (including bank codes): SWIFT/BIC code PASCITMMFOG

IBAN code: IT42K0103015700 000001213251

TREASURE 634476 - Consortium Agreement

13. ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA (UNIBO) Name of bank: UNICREDIT BANKA SPA

Address of branch: Via Trauzzi Alberto, 15/A, IT-40127 Bologna Full name of the account Alma mater studiorum, Universita di Bologna, Dipartimento di Scienze holder: e Tecnologie Agro-Alimentari Full account number R 02008 02428 000102272872 (including bank codes): Reference 309018

SWIFT/BIC code UNCRITM1MR9

IBAN code: IT88R0200802428000102272872

14. STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI (SSICA) Name of bank: CARIPARMA CREDIT AGRICOLE SPA

Address of branch: Piazza Garibaldi, 1, 43121 Parma, Italy Full name of the account STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE holder: ALIMENTARI Full account number 06230 12700 000036171783 (including bank codes): SWIFT/BIC code CRPPIT2P452

IBAN code: IT25D0623012700 000036171783

15. LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (LUHS) Name of bank: AB DNB

Address of branch: J. Basanavičiaus g. 26, Vilnius Full name of the account LIETUVOS SVEIKATOS MOKSLU UNIVERSITETAS (Lithuanian University holder: of Health Sciences) Full account number 4010044800020070 (including bank codes): SWIFT/BIC code AGBLLT2X

IBAN code: LT594010044800020070

TREASURE 634476 - Consortium Agreement

16. INSTITUTO POLITECNICO DE VIANA DE CASTELO (IPVC) Name of bank: Instituto de Gestão da Tesouraria e do Credito Publico I P

Address of branch: Av. da República, 57 - 6º, 1050 - 189 Lisboa. PORTUGAL Full name of the account INSTITUTO POLITÉCNICO VIANA DO CASTELO - IPVC holder: Full account number 0781 0112 0000000770348 (including bank codes): SWIFT/BIC code IGCPPTPL

IBAN code: PT50078101120000000770348

17. UNIVERSIDADE DE EVORA (UEVORA) PCUP Name of bank: Millennium BCP

Address of branch: Rua do Conde de Serra da Tourega, 1, 7000-875 Évora, Portugal Full name of the account Conselho de Gestão da Universidade de Évora holder: Full account number 0033 00000020800699505 (including bank codes): SWIFT/BIC code BCOMPTPL

IBAN code: PT50 0033 00000020800699505

18. INSTITUT ZA STOCARSTVO BEOGRAD-ZEMUN (IAH) Name of bank: AIK BANKA AD NIS

Address of branch: Nikole Pasića 42, 18000 Niš, SERBIA Full name of the account INSTITUT ZA STOČARSTVO holder: Full account number 105050120000062319 (including bank codes): SWIFT/BIC code AIKBRS22

IBAN code: RS35105050120000062319

TREASURE 634476 - Consortium Agreement

19. FACULTY OF AGRICULTURE - UNIVERSITY OF BELGRADE (UNIBG) Name of bank: NATIONAL BANK OF SERBIA

Address of branch: Kralja Petra 12, 11000 Beograd, Serbia Full name of the account UNIVERSITY OF BELGRADE – FACULTY OF AGRICULTURE holder: Full account number 908500100014415751 (including bank codes): SWIFT/BIC code NBSRRSBG

IBAN code: RS35908500100014415751

20. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA (INIA) Name of bank: BANCO DE ESPAÑA

Address of branch: Alcala 50, 28014 Madrid, Spain Full name of the account holder: Tesoro publico aportaciones de la UE Full account number (including bank codes): 9000 0001 20 0253 107033 Reference INIA-TREASURE

SWIFT/BIC code ESPBESMMXXX

IBAN code: ES4490000001200253107033

21. AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) Name of bank: BANCO DE ESPAÑA

Address of branch: Alcala 50, 28014 Madrid, Spain Full name of the account Tesoro publico aportaciones de la UE holder: Full account number 9000 0001 20 0253 107033 (including bank codes): Reference CSIC-TREASURE

SWIFT/BIC code ESPBESMM

IBAN code: ES4490000001200253107033

TREASURE 634476 - Consortium Agreement

22. CENTRE DE RECERCA EN ECONOMIA I DESENVOLUPAMENT AGROALIMENTARI-UPC- IRTA (CREDA) BBVA Name of bank: BANCO BILBAO VIZCAYA ARGENTARIA, S.A. Plaza Cataluña, 5 – 1st floor Address of branch: 08002 Barcelona Spain Full name of the account FUNDACIO CREDA holder: Full account number 01826035460201507937 (including bank codes): SWIFT/BIC code BBVAESMMXXX

IBAN code: ES1301826035460201507937

23. CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE EXTREMADURA (CICYTEX) Name of bank: CAJA RURAL DE EXTREMADURA, S.C.C.

Address of branch: 1 Avda. Lusitania, 2, 06800 Merida (Badajoz), Spain Full name of the account CENTRO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS DE holder: EXTREMADURA (CICYTEX) Full account number 3009 0045 1223 4706 7221 (including bank codes): SWIFT/BIC code BCOEESMM009

IBAN code: ES5630090045122347067221

24. INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES (IRTA) Name of bank: BANCO DE SABADELL, S.A.

Address of branch: Avinguda Diagonal 407, 2, 08008 Barcelona, Spain Full name of the account INSTITUT DE RECERCA I TECNOLOGIA AGROALIMENTARIES holder: Full account number 00815172820001052013 (including bank codes): SWIFT/BIC code BSABESBB

IBAN code: ES7300815172820001052013

TREASURE 634476 - Consortium Agreement

25. ASOCIACION ESPANOLA DE CRIADORES DE CERDO IBERICO (AECERIBER) Name of bank: IBERCAJA BANCO, S.A.

Address of branch: Av. Antonio Chacón, 2, 06300 Zafra (Badajoz), Spain Full name of the account AECERIBER holder: Full account number 2085 4518 03 0330506237 (including bank codes): SWIFT/BIC code CAZRES2Z

IBAN code: ES7220854518030330506237

TREASURE 634476 - Consortium Agreement

Attachment 7: Material Transfer Agreement

Simple Letter Agreement for the Transfer of Materials

In response to the RECIPIENT’s request for the MATERIAL [insert description]

the PROVIDER asks that the RECIPIENT and the RECIPIENT SCIENTIST agree to the following before the RECIPIENT receives the MATERIAL: - The above MATERIAL is the property of the PROVIDER and is made available exclusively in the frame of the tasks described in TREASURE 634476 project. - THIS MATERIAL IS NOT FOR USE IN HUMAN SUBJECTS. - The MATERIAL will be used for not-for-profit research purposes only. - The MATERIAL will not be further distributed to others without the PROVIDER’s written consent. The RECIPIENT shall refer any request for the MATERIAL to the PROVIDER. To the extent supplies are available, the PROVIDER or the PROVIDER SCIENTIST agree to make the MATERIAL available, under a separate Simple Letter Agreement to other scientists for teaching or not-for-profit research purposes only. - The RECIPIENT agrees to acknowledge the source of the MATERIAL in any publications reporting use of it. - Any MATERIAL delivered pursuant to this Agreement is understood to be experimental in nature and may have hazardous properties. THE PROVIDER MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE MATERIAL WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. Unless prohibited by law, RECIPIENT assumes all liability for claims for damage against it by third parties which may arise from the use, storage or disposal of the MATERIAL except that, to the extent permitted by law, the PROVIDER shall be liable to the RECIPIENT when the damage is caused by the gross negligence or wilful misconduct of the PROVIDER. - The RECIPIENT agrees to use the MATERIAL in compliance with all applicable statutes and regulations. - The MATERIAL is provided at no cost.

The PROVIDER, RECIPIENT and RECIPIENT SCIENTIST must sign both copies of this letter and return one signed copy to the PROVIDER. The PROVIDER will then send the MATERIAL.

PROVIDER INFORMATION and AUTHORISED SIGNATURE Provider Scientist: Provider Organisation: Address: Name of Authorised Official:

TREASURE 634476 - Consortium Agreement

Title of Authorised Official:

Certification of Authorised Official: This Simple Letter Agreement __has / __has not [check one] been modified. If modified, the modifications are attached.

Signature of Authorised Official and Date

RECIPIENT INFORMATION and AUTHORISED SIGNATURE Provider Scientist: Provider Organisation: Address: Name of Authorised Official: Title of Authorised Official: Signature of Authorised Official: Date:

Certification of Recipient Scientist: I have read and understood the conditions outlined in this Agreement and I agree to abide by them in the receipt and use of the MATERIAL.

Signature of Recipient Scientist and Date