Product datasheet Anti-BCL6 Antibody Catalog Number: A00142-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-BCL6 Antibody Name BCL6 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,IHC-F,ICC,FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence of human BCL6 (ASPADSCIQFTRHASDVLLNLNRLRSRDILTD). Purification Immunogen affinity purified. Observed MW 79KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section: 1:50-400 Immunohistochemistry in frozen section: 1:50-400 Immunocytochemistry in fixed cells: 1:50-400 Flow Cytometry: 1-3μg/1x106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information B-cell lymphoma 6 is a protein that in humans is encoded by the BCL6 gene. Like BCL2, BCL3, BCL5, BCL7A, BCL9 and BCL10, it has clinical significance in lymphoma. The protein encoded by this gene is an evolutionarily conserved zinc finger transcription factor and contains an N-terminal POZ/BTB domain. This protein acts as a sequence-specific repressor of transcription and has been shown to modulate the STAT- dependent Interleukin 4 (IL-4) responses of B cells. This protein can interact with several corepressor complexes to inhibit transcription. This gene is found to be frequently translocated and hypermutated in diffuse large B cell lymphoma (DLBCL) and contributes to the pathogenesis of DLBCL. An exon 7 skipping splice variant encodes a shorter form of the protein which lacks the first two zinc fingers of the DNA binding domain.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-BCL6 Antibody Catalog Number: A00142-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-BCL6 Antibody Catalog Number: A00142-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Western blot analysis of Bcl6 using anti- analysis of Bcl6 using anti- Bcl6 antibody (A00142-1). Bcl6 antibody (A00142-1). The sample well of each The sample well of each lane was loaded with lane was loaded with 50ug of sample under 50ug of sample under reducing conditions. Lane reducing conditions. Lane 1: human Raji whole cell 1: rat testis tissue lysate,Lane 2: human lysate,Lane 2: rat spleen MCF-7 whole cell tissue lysate,Lane 3: rat lysate,Lane 3: human brain tissue lysate,Lane 4: placenta tissue mouse testis tissue lysate,Lane 4: human lysate,Lane 5: mouse A549 whole cell spleen tissue lysate,Lane lysate,Lane 5: human 6: mouse brain tissue Caco-2 whole cell lysate. -Bcl6 antigen lysate,Lane 6: human affinity purified U2OS whole cell lysate. polyclonal antibody -Bcl6 antigen affinity (Catalog # A00142-1) at purified polyclonal 0.5 μg/mL overnight at antibody (Catalog # 4°C, then washed with A00142-1) at 0.5 μg/mL TBS-0.1%Tween 3 times overnight at 4°C, then with 5 minutes each and washed with probed with a goat anti- TBS-0.1%Tween 3 times rabbit IgG-HRP with 5 minutes each and secondary antibody . The probed with a goat anti- signal is developed using rabbit IgG-HRP an Enhanced secondary antibody . The Chemiluminescent signal is developed using detection (ECL) kit an Enhanced (Catalog # EK1002) A Chemiluminescent specific band was detection (ECL) kit detected for Bcl6 at (Catalog # EK1002) A approximately 88KD. The specific band was expected band size for detected for Bcl6 at Bcl6 is at 79KD. approximately 88KD. The expected band size for Bcl6 is at 79KD.

Figure 3. IHC analysis of Figure 4. IHC analysis of Bcl6 using anti-Bcl6 Bcl6 using anti-Bcl6 antibody (A00142-1). antibody (A00142-1). Bcl6 was detected in Bcl6 was detected in paraffin-embedded paraffin-embedded section of human tonsil section of mouse spleen tissues. anti-Bcl6 tissues. anti-Bcl6 Antibody (A00142-1) Antibody (A00142-1)

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3 Product datasheet Anti-BCL6 Antibody Catalog Number: A00142-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

overnight at 4°C. overnight at 4°C. Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody The secondary antibody The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC)(Catalog # (SABC)(Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 4

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