Supporting Information for Proteomics DOI 10.1002/pmic.200701009

Julia Smith, Isaac Matus, Daniel Beard and Andrew Greene

Differential expression of cardiac mitochondrial proteins

ª 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com Differential expression of cardiac mitochondrial proteins

Supporting Information Table 1 Protein Score Peptide Scan Total Total TIC Total Percent MWT Count Count Xcorr Mod. coverage

ATPB_MOUSE (P56480) 1.17E+09 20 273 1005.60 2.12E+06 146 52.94 56215 ATP synthase beta chain, mitochondrial precursor (EC 3.6.3.14 ATPA_MOUSE (Q03265) 1.06E+08 11 150 515.10 1.25E+06 70 28.05 59380 ATP synthase alpha chain, mitochondrial precursor (EC 3.6.3. ACON_MOUSE (Q99KI0) 2.42E+07 14 82 326.32 6.46E+05 38 26.25 85208 Aconitate hydratase, mitochondrial precursor (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitas MDHM_MOUSE (P08249) 1.52E+07 10 80 291.28 6.52E+05 46 39.82 35224 , mitochondrial precursor (EC 1.1.1. ADT1_MOUSE (P48962) 5.10E+06 3 84 272.17 7.44E+05 46 17.75 32384 ADP/ATP translocase 1 (Adenine nucleotide translocator 1) (ANT 1) (ADP,ATP carrier protein 1) (Solute carrier family 25 member 4) (ADP,ATP carrier protein, heart/skeletal muscle isoform T1) (mAN

CH60_CRIGR (P18687) 60 2.14E+06 4 49 225.09 4.85E+05 31 11.60 60654 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein

ODPA_MOUSE (P35486) 3.89E+06 8 51 213.35 4.47E+05 32 19.45 82386 E1 component alpha subunit, somatic form, mitochondrial precursor (EC 1.2.4.1) (PDHE1-A type COX2_RAT (P00406) 3.29E+05 1 45 191.10 3.83E+05 26 8.93 25699 subunit 2 (EC 1.9.3.1) (Cytochrome c oxidase polypeptide I Differential expression of cardiac mitochondrial proteins

ETFA_MOUSE (Q99LC5) 7.71E+05 4 34 186.42 3.04E+05 15 20.00 34784 Electron transfer flavoprotein alpha-subunit, mitochondrial precursor (Alpha-ET ACADL_MOUSE (P51174) 1.57E+06 5 49 182.63 3.51E+05 24 17.14 47620 Acyl-CoA dehydrogenase, long-chain specific, mitochondrial precursor (EC 1.3.99.13) (LC COX5A_MOUSE (P12787) 9.24E+05 4 40 171.01 3.38E+05 18 21.99 15689 Cytochrome c oxidase polypeptide Va, mitochondrial precursor (EC 1.9.3 ODO1_MOUSE (Q60597) 2- 1.45E+06 7 43 161.87 2.98E+05 20 10.74 115757 oxoglutarate dehydrogenase E1 component, mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogena ODPB_MOUSE (Q9D051) 7.49E+05 6 32 132.72 2.94E+05 16 26.76 38598 Pyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1 MYG_RAT (Q9QZ76) 3.54E+05 4 27 132.60 2.47E+05 13 42.28 16694 Myoglobin THIM_MOUSE (Q8BWT1) 3- 5.07E+05 5 34 128.41 2.32E+05 16 21.88 41545 ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Beta-ketothiolase) (Acetyl- CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiola

NUAM_MOUSE (Q91VD9) 4.51E+05 4 34 127.57 2.60E+05 14 9.13 79447 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-75Kd) (CI-75

KCRS_MOUSE (Q6P8J7) 5.80E+05 6 29 123.63 2.70E+05 16 30.84 47133 Creatine kinase, sarcomeric mitochondrial precursor (EC 2.7.3.2) (S-Mt CYC_MOUSE (P62897) 4.25E+05 4 30 118.11 3.00E+05 16 40.00 11131 Cytochrome c, soma Differential expression of cardiac mitochondrial proteins

NNTM_MOUSE (Q61941) 4.76E+05 6 27 114.23 2.57E+05 12 9.06 113420 NAD(P) transhydrogenase, mitochondrial precursor (EC 1.6.1.2) (Pyridine nucleotide transhydrogenase) (Nicotinamide nucleotide transhydrogena

NUEM_MOUSE (Q9DC69) 1.77E+05 4 24 98.74 1.87E+05 10 15.55 42134 NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-39KD) (CI-39

CY1_MOUSE (Q9D0M3) 7.92E+04 2 22 96.74 1.86E+05 18 12.46 35006 Cytochrome c1, protein, mitochondrial precursor (Cytochrome c ACADS_MOUSE (Q07417) 1.47E+05 4 22 96.70 1.73E+05 20 18.14 44541 Acyl-CoA dehydrogenase, short-chain specific, mitochondrial precursor (EC 1.3.99.2) (SCAD) (Butyryl- CoA dehydrogena ETFD_MOUSE (Q921G7) 7.46E+04 2 23 91.60 1.77E+05 23 6.37 67790 Electron transfer flavoprotein- ubiquinone oxidoreductase, mitochondrial precursor (EC 1.5.5.1) (ETF-QO) (ETF- ubiquinone oxidoreductase) (ETF dehydrogenase) (Electron-transferring- flavoprotein dehydrogena

AT5F1_MOUSE (Q9CQQ7) 4.64E+04 1 28 89.74 1.85E+05 13 5.95 28608 ATP synthase B chain, mitochondrial precursor (EC 3.6.3. IDHP_MOUSE (P54071) 4.25E+05 9 27 82.41 2.12E+05 14 17.34 58437 [NADP], mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase) (IDH) (NADP(+)-specific ICDH) (IDP) (ICD Differential expression of cardiac mitochondrial proteins

HCD2_RAT (O70351) 3- 6.00E+04 2 22 82.26 1.66E+05 11 15.18 26890 hydroxyacyl-CoA dehydrogenase type II (EC 1.1.1.35) (Type II HADH) (3- hydroxy-2-methylbutyryl-CoA dehydrogenase) (EC 1.1.1.178) (Endoplasmic reticulum-associated amyloid beta-peptide binding protei

ATP5J_RAT (P21571) ATP 2.81E+04 1 19 81.67 1.81E+05 9 18.10 12280 synthase coupling factor 6, mitochondrial precursor (EC 3.6.3.14) (ATPase subunit F DHSA_MOUSE (Q8K2B3) 8.26E+04 3 19 79.84 1.81E+05 6 9.08 72336 [ubiquinone] flavoprotein subunit, mitochondrial precursor (EC 1.3.5.1) (Fp) (Flavoprotein subunit of complex I

FUMH_MOUSE (P97807) 6.28E+04 3 19 73.85 1.49E+05 10 10.74 54090 Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) () (EF ECHM_RAT (P14604) Enoyl- 2.95E+04 2 14 72.03 1.47E+05 14 10.14 31137 CoA hydratase, mitochondrial precursor (EC 4.2.1.17) (Short chain enoyl-CoA hydratase) (SCEH) (Enoyl- CoA hydratase MPCP_MOUSE (Q8VEM8) 6.69E+04 4 17 70.67 1.39E+05 8 15.58 39290 Phosphate carrier protein, mitochondrial precursor (PTP) (Solute carrier family 25 member UQCR2_MOUSE (Q9DB77) 7.57E+04 4 18 68.99 1.52E+05 9 14.48 47879 Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial precursor (EC 1.10.2.2) (Complex III subunit CMC2_MOUSE (Q9QXX4) 2.12E+04 2 13 67.62 1.21E+05 5 7.88 74266 Calcium-binding mitochondrial carrier protein Aralar2 (Mitochondrial aspartate glutamate carrier 2) (Solute carrier family 25 member 13) (Citri Differential expression of cardiac mitochondrial proteins

D3D2_RAT (P23965) 3,2- 4.66E+04 2 19 67.21 1.82E+05 11 11.93 31942 trans-enoyl-CoA isomerase, mitochondrial precursor (EC 5.3.3.8) (Dodecenoyl-CoA isomerase) (Delta(3),delta(2)- enoyl-CoA isomerase) (D3,D2-enoyl-CoA isomera

CMC1_MOUSE (Q8BH59) 8.20E+04 5 16 66.36 1.54E+05 7 14.86 74300 Calcium-binding mitochondrial carrier protein Aralar1 (Mitochondrial aspartate glutamate carrier 1) (Solute carrier family 25 member

ODP2_RAT (P08461) 7.29E+04 5 17 66.15 1.30E+05 4 15.76 58539 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12) (E2) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (PDC-E2) (70 kDa mitochondrial autoantigen o

HBA_RAT (P01946) 7.52E+04 4 19 61.92 1.60E+05 8 45.26 14751 Hemoglobin alpha-1/2 subunit (Hemoglobin alpha-1/2 chain) (Alpha-1/2-glob ATPO_MOUSE (Q9DB20) 5.81E+04 2 23 59.80 2.11E+05 6 11.96 22991 ATP synthase O subunit, mitochondrial precursor (EC 3.6.3.14) (Oligomycin sensitivity conferral protein) (OS HBB1_RAT (P02091) 6.36E+03 1 11 59.49 9.72E+04 5 13.38 15421 Hemoglobin beta-1 subunit (Hemoglobin beta-1 chain) (Beta-1-globin) (Hemoglobin beta chain, major-fo ECHB_MOUSE (Q99JY0) 3.99E+04 4 16 58.77 1.06E+05 13 15.29 50999 Trifunctional beta subunit, mitochondrial precursor (TP-beta) [Includes: 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta- ketothiolas Differential expression of cardiac mitochondrial proteins

NIDM_MOUSE (Q9DCS9) 2.10E+04 2 15 57.01 1.23E+05 8 19.30 20680 NADH-ubiquinone oxidoreductase PDSW subunit (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-PDSW) (CI-PD CX6B1_MOUSE (P56391) 9.68E+03 1 15 55.00 1.17E+05 15 17.28 9643 Cytochrome c oxidase subunit VIb isoform 1 (EC 1.9.3.1) (COX VIb AATM_MOUSE (P05202) 4.01E+04 3 16 53.79 1.55E+05 9 11.50 47084 Aspartate aminotransferase, mitochondrial precursor (EC 2.6.1.1) (Transaminase A) (Glutamate oxaloacetate transaminase SCOT_MOUSE (Q9D0K2) 4.08E+04 4 16 50.02 1.28E+05 12 18.60 55688 Succinyl-CoA:3-ketoacid- coenzyme A transferase 1, mitochondrial precursor (EC 2.8.3.5) (Somatic-type succinyl CoA:3-oxoacid CoA- transferase) (Scot

HCDH_RAT (Q9WVK7) Short 6.24E+03 1 12 47.71 1.09E+05 5 9.68 34029 chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (EC 1.1.1.35) (HCDH) (Medium and short chain L-3- hydroxyacyl-coenzyme A dehydrogena

NUDM_MOUSE (Q99LC3) 1.67E+04 3 12 42.93 1.08E+05 9 13.07 40363 NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-42KD) (CI-42K

N7BM_MOUSE (Q7TMF3) 4.45E+03 1 12 42.18 8.80E+04 5 12.06 16699 NADH-ubiquinone oxidoreductase subunit B17.2 (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-B17.2) (CI-B17.2) (CIB17 ACSL1_MOUSE (P41216) 3.13E+03 1 11 40.67 7.00E+04 10 2.59 77570 Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.1.3) (Long- chain acyl-CoA synthetase 1) (LACS Differential expression of cardiac mitochondrial proteins

UCRI_MOUSE (Q9CR68) 3.30E+03 1 10 39.35 8.39E+04 4 5.56 29113 Ubiquinol-cytochrome c reductase iron-sulfur subunit, mitochondrial precursor (EC 1.10.2.2) (Rieske iron-sulfur protein) (RI SUCA_RAT (P13086) 2.93E+03 1 10 37.13 7.89E+04 10 4.86 34660 Succinyl-CoA ligase [GDP- forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alp

MA32_MOUSE (O35658) 3.91E+03 2 8 35.57 6.87E+04 6 20.44 30644 Complement component 1, Q subcomponent-binding protein, mitochondrial precursor (Glycoprotein gC1qBP) (GC1q-R prote ALDH2_MESAU (P81178) 8.58E+03 3 11 32.84 7.92E+04 4 8.87 54006 Aldehyde dehydrogenase, mitochondrial (EC 1.2.1.3) (ALDH class 2) (ALDH1) (ALDH- CISY_MOUSE (Q9CZU6) 1.66E+03 1 8 31.71 6.54E+04 4 3.48 51407 , mitochondrial precursor (EC 2.3.3 EFTU_MOUSE (Q8BFR5) 1.82E+03 1 9 30.63 6.62E+04 2 3.13 49108 Elongation factor Tu, mitochondrial precur ACADV_RAT (P45953) Acyl- 4.20E+03 3 7 30.14 6.64E+04 3 7.53 70382 CoA dehydrogenase, very- long-chain specific, mitochondrial precursor (EC 1.3.99.-) (VLC ACTA_MOUSE (P62737) 4.90E+03 3 8 29.41 6.94E+04 3 17.16 41632 Actin, aortic smooth muscle (Alpha-actin TRY1_RAT (P00762) Anionic 1.12E+03 1 6 28.69 6.50E+04 3 8.26 25704 trypsin I precursor (EC 3.4.21.4) (Pretrypsinogen ODPA_MOUSE (P35486) 2.91E+03 2 8 25.57 7.11E+04 3 5.70 42959 Pyruvate dehydrogenase E1 component alpha subunit, somatic form, mitochondrial precursor (EC 1.2.4.1) (PDHE1-A type Differential expression of cardiac mitochondrial proteins

NUCM_MOUSE (Q91WD5) 2.41E+03 2 8 25.20 5.99E+04 5 8.71 52242 NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-49KD) (CI-49

VDAC1_MOUSE (Q60932) 7.09E+03 4 9 25.19 7.81E+04 3 18.84 32006 Voltage-dependent anion- selective channel protein 1 (VDAC-1) (mVDAC1) (mVDAC5) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal por

ATP5H_MOUSE (Q9DCX2) 3.34E+03 3 8 23.90 5.82E+04 1 10.90 18263 ATP synthase D chain, mitochondrial (EC 3.6.3. FABPH_RAT (P07483) Fatty 9.32E+02 1 7 23.50 5.66E+04 2 10.94 14316 acid-binding protein, heart (H- FABP) (Heart-type fatty acid- binding prote DLDH_MOUSE (O08749) 3.56E+03 4 7 21.91 5.80E+04 5 12.28 53839 Dihydrolipoyl dehydrogenase, mitochondrial precursor (EC 1.8.1.4) (Dihydrolipoamide dehydrogena NUGM_MOUSE (Q9DCT2) 1.31E+03 2 6 21.33 5.10E+04 3 5.79 29850 NADH-ubiquinone oxidoreductase 30 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-30KD) (CI-30

NUKM_MOUSE (Q9DC70) 7.57E+02 1 7 20.88 5.18E+04 7 12.27 24208 NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-20KD) (CI-20KD) (PSST subun

ACADM_MOUSE (P45952) 6.07E+02 1 5 20.29 5.99E+04 5 7.43 46077 Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MC IMMT_MOUSE (Q8CAQ8) 2.33E+02 1 4 18.71 3.11E+04 0 2.26 83516 Mitochondrial inner membrane protein (Mitofil Differential expression of cardiac mitochondrial proteins

NUHM_MOUSE (Q9D6J6) 2.08E+02 1 4 18.23 2.86E+04 4 6.97 27075 NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99 NUBM_MOUSE (Q91YT0) 5.86E+02 2 4 17.78 4.12E+04 4 11.96 50606 NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-51KD) (CI-51KD) (NADH dehydrogenase flavoprotein

CNOT4_MOUSE (Q8BT14) 6.35E+02 1 8 17.59 4.51E+04 8 1.75 63204 CCR4-NOT transcription complex subunit 4 (EC 6.3.2.- ) (E3 ubiquitin protein ligase CNOT4) (CCR4-associated factor 4) (Potential transcriptional repressor NOT4

MMSA_RAT (Q02253) 8.49E+02 3 5 17.55 3.22E+04 0 8.46 57596 Methylmalonate- semialdehyde dehydrogenase [acylating], mitochondrial precursor (EC 1.2.1.27) (MMSDH) (Malonate-semialdehyde dehydrogenase [acylating]) (EC 1.2.1.1 PRAX_MOUSE (O55103) 6.73E+02 1 7 17.50 5.49E+04 3 1.08 147434 Periaxin N4BM_MOUSE (Q9CQ54) 3.64E+02 1 5 17.48 4.17E+04 3 0.00 13812 NADH-ubiquinone oxidoreductase subunit B14.5b (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-B14.5b) (CI-B14. COX41_RAT (P10888) 1.99E+02 1 5 14.41 2.76E+04 1 7.27 19073 Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor (EC 1.9.3.1) (COX IV-1) (Cytochrome c oxidase polypeptide Differential expression of cardiac mitochondrial proteins

DHSB_MOUSE (Q9CQA3) 1.72E+02 1 5 14.39 2.40E+04 2 5.04 31560 Succinate dehydrogenase [ubiquinone] iron-sulfur protein, mitochondrial precursor (EC 1.3.5.1) (Ip) (Iron-sulfur subunit of complex

HBB2_RAT (P11517) 1.34E+02 1 3 13.84 3.22E+04 0 11.97 15424 Hemoglobin beta-2 subunit (Hemoglobin beta-2 chain) (Beta-2-globin) (Hemoglobin beta chain, minor-fo THIL_MOUSE (Q8QZT1) 3.96E+02 2 4 13.24 3.74E+04 2 4.04 44576 Acetyl-CoA acetyltransferase, mitochondrial precursor (EC 2.3.1.9) (Acetoacetyl-CoA thiolas

ALBU_MERUN (O35090) 4.93E+02 2 5 12.90 3.82E+04 2 4.46 68687 Serum albumin precur SIRT3_MOUSE (Q8R104) 2.05E+02 1 4 12.63 4.05E+04 1 6.72 28424 NAD-dependent deacetylase sirtuin-3 (EC 3.5.1.-) (SIR2- like protein 3) (mSIR2 AT2A1_MOUSE (Q8R429) 7.99E+01 1 3 12.20 2.18E+04 0 1.52 109128 Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC 3.6.3.8) (Calcium pump 1) (SERCA1) (SR Ca(2+)- ATPase 1) (Calcium- transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform) (Endoplasmic reticulum

IDHC_MICME (Q9Z2K9) 7.29E+02 3 5 11.86 4.10E+04 1 8.76 46438 Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Oxalosuccinate decarboxylase) (IDH) (NADP(+)-specific ICDH) (ID UCR6_MOUSE (Q9D855) 1.03E+02 1 3 11.83 2.90E+04 2 12.26 13070 Ubiquinol-cytochrome c reductase complex 14 kDa protein (EC 1.10.2.2) (Complex III subunit Differential expression of cardiac mitochondrial proteins

NUPM_MOUSE (Q9DCJ5) 2.65E+02 2 4 11.58 2.86E+04 1 15.57 19476 NADH-ubiquinone oxidoreductase 19 kDa subunit (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-19KD) (CI-19KD) (Complex I-PGIV) (CI-PG

VDAC3_MOUSE (Q60931) 2.75E+02 1 5 11.44 4.80E+04 0 3.58 30440 Voltage-dependent anion- selective channel protein 3 (VDAC-3) (mVDAC3) (Outer mitochondrial membrane protein porin ODO2_MOUSE (Q9D2G2) 2.90E+01 1 2 11.40 1.27E+04 1 4.67 48654 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC 2.3.1.61) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex) (E2)

M2OM_MOUSE (Q9CR62) 1.85E+02 1 4 11.27 4.11E+04 3 7.12 33768 Mitochondrial 2- oxoglutarate/malate carrier protein (OGCP) (Solute carrier family 25 member ELMO2_MOUSE (Q8BHL5) 2.17E+02 1 5 11.22 3.87E+04 5 1.24 83530 Engulfment and cell motility protein 2 (CED-12 homolog ETFA_HUMAN (P13804) 4.62E+01 1 2 10.89 2.12E+04 0 5.15 34824 Electron transfer flavoprotein alpha-subunit, mitochondrial precursor (Alpha-ET GRP75_CRIGR (O35501) 2.15E+02 2 4 10.42 2.58E+04 1 4.59 73346 Stress-70 protein, mitochondrial precursor (75 kDa glucose-regulated protein) (GRP AT10A_MOUSE (O54827) 1.68E+02 1 4 10.27 4.09E+04 1 1.13 168513 Probable phospholipid- transporting ATPase VA (EC 3.6.3.1) (P-locus fat- associated ATPas Differential expression of cardiac mitochondrial proteins

ACDL_HUMAN (P28330) 2.41E+02 2 4 10.20 2.95E+04 0 6.07 47543 Acyl-CoA dehydrogenase, long-chain specific, mitochondrial precursor (EC 1.3.99.13) (LCAD N5BM_RAT (Q80W89) 7.18E+01 1 3 10.15 2.36E+04 3 24.82 14531 NADH-ubiquinone oxidoreductase subunit B14.7 (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-B14.7) (CI-B14 LDHB_MOUSE (P16125) L- 3.16E+01 1 2 9.80 1.61E+04 1 6.08 36086 lactate dehydrogenase B chain (EC 1.1.1.27) (LDH-B) (LDH heart subunit) (LDH GIT1_MOUSE (Q68FF6) 2.97E+02 2 4 9.79 3.79E+04 4 4.44 84917 ARF GTPase-activating protein GIT1 (G protein- coupled receptor kinase- interactor 1) (GRK-interacting protein ARK72_RAT (Q8CG45) 1.00E+02 1 3 9.77 3.42E+04 3 8.82 40210 Aflatoxin B1 aldehyde reductase member 2 (EC 1.-.- .-) (rAFA ACOT2_MOUSE (Q9QYR9) 4.00E+01 1 2 9.69 2.06E+04 0 3.78 49438 Acyl-coenzyme A thioesterase 2, mitochondrial precursor (EC 3.1.2.2) (Acyl- CoA thioesterase 2) (Acyl coenzyme A thioester hydrolase) (Very-long-chain acyl-CoA thioesterase) (MTE-

AT2A2_MOUSE (O55143) 3.91E+01 1 2 9.33 2.09E+04 2 1.44 114439 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (EC 3.6.3.8) (Calcium pump 2) (SERCA2) (SR Ca(2+)- ATPase 2) (Calcium- transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform) (Endoplasmic reticulum

NUML_MOUSE (Q62425) 5.64E+01 1 3 9.17 2.05E+04 2 15.38 8968 NADH-ubiquinone oxidoreductase MLRQ subunit (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-MLRQ) (CI-ML Differential expression of cardiac mitochondrial proteins

RIR1_MOUSE (P07742) 1.36E+02 2 3 9.14 2.48E+04 3 2.79 89973 Ribonucleoside-diphosphate reductase large subunit (EC 1.17.4.1) (Ribonucleoside- diphosphate reductase M1 subunit) (Ribonucleotide reductase large cha

UCR10_MOUSE (Q8R1I1) 6.24E+01 1 3 8.99 2.31E+04 1 28.33 7075 Ubiquinol-cytochrome c reductase complex 7.2 kDa protein (EC 1.10.2.2) (Cytochrome C1, nonheme 7 kDa protein) (Complex III subunit X) (7.2 kDa cytochrome c1-associated protein subun

PRDX3_MOUSE (P20108) 3.31E+01 1 2 8.88 1.87E+04 0 5.53 27764 Thioredoxin-dependent peroxide reductase, mitochondrial precursor (EC 1.11.1.15) (Perioredoxin-3) (Antioxidant protein 1) (AOP- 1) (MER5 protein) (PRX I

ATPG_MOUSE (Q91VR2) 2.83E+02 2 4 8.74 4.05E+04 1 7.82 32588 ATP synthase gamma chain, mitochondrial precursor (EC 3.6.3. MYO5B_MOUSE (P21271) 6.65E+01 1 4 8.73 1.91E+04 1 0.97 82859 Myosin-5B (Myosin Vb) NPT2B_MOUSE (Q9DBP0) 2.52E+02 3 3 8.68 3.23E+04 3 7.79 75899 Sodium-dependent phosphate transport protein 2B (Sodium/phosphate cotransporter 2B) (Na(+)/Pi cotransporter 2B) (Sodium- phosphate transport protein 2B) (Na(+)-dependent phosphate cotransporter 2B) (NaPi-2b) (Solute carrier family

NUIM_MOUSE (Q8K3J1) 3.20E+01 1 2 8.64 1.85E+04 2 8.65 23607 NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-23KD) (CI-23KD) (TYKY subun

H2A1_MOUSE (P22752) 3.41E+01 1 2 8.62 1.98E+04 1 23.20 13692 Histone H2A typ Differential expression of cardiac mitochondrial proteins

EF1A2_MOUSE (P62631) 2.29E+01 1 2 8.43 1.36E+04 0 5.23 50199 Elongation factor 1-alpha 2 (EF-1-alpha-2) (Elongation factor 1 A-2) (eEF1A-2) (Statin PDP2_RAT (O88484) 7.89E+01 1 3 8.06 3.26E+04 2 3.42 59341 [Pyruvate dehydrogenase [lipoamide]]-phosphatase 2, mitochondrial precursor (EC 3.1.3.43) (PDP 2) (Pyruvate dehydrogenase phosphatase, catalytic subunit 2) (PDPC

CAP2_MOUSE (Q9CYT6) 5.20E+01 1 3 8.01 2.16E+04 0 5.51 52548 Adenylyl cyclase-associated protein 2 (CAP ETFA_RAT (P13803) 4.23E+01 2 2 7.75 1.37E+04 0 11.78 34849 Electron transfer flavoprotein alpha-subunit, mitochondrial precursor (Alpha-ETF LAMA1_MOUSE (P19137) 9.10E+01 2 3 7.66 1.98E+04 1 1.43 337851 Laminin alpha-1 chain precursor (Laminin A cha ES1_MOUSE (Q9D172) ES1 3.08E+01 1 2 7.38 2.09E+04 2 5.73 27805 protein homolog, mitochondrial precur OPA1_MOUSE (P58281) 2.92E+01 1 2 7.17 2.04E+04 0 2.93 110955 Dynamin-like 120 kDa protein, mitochondrial precursor (Large GTP binding protein) (Larg ACOT1_MOUSE (O55137) 2.13E+01 1 2 7.09 1.50E+04 1 6.99 45799 Acyl-coenzyme A thioesterase 1 (EC 3.1.2.2) (Acyl-CoA thioesterase 1) (Inducible cytosolic acyl- coenzyme A thioester hydrolase) (Long chain acyl- CoA thioester hydrolase) (Long chain acyl-CoA hydrolase) (CTE

H2AY_RAT (Q02874) Core 4.91E+01 2 2 7.03 1.75E+04 1 13.11 39090 histone macro-H2A.1 (Histone macroH2A1) (mH2A1) (H2A.y) (H2A ACHA4_MOUSE (O70174) 3.05E+01 1 2 6.89 2.22E+04 2 4.32 70005 Neuronal acetylcholine receptor protein, alpha-4 subunit precur Differential expression of cardiac mitochondrial proteins

COXW_HUMAN (Q7KZN9) 2.77E+01 1 2 6.85 2.02E+04 2 5.39 45903 Cytochrome c oxidase assembly protein COX15 homolo LAMB1_MOUSE (P02469) 5.26E+01 1 3 6.79 2.58E+04 2 0.62 196589 Laminin beta-1 chain precursor (Laminin B1 cha KCNA5_HUMAN (P22460) 3.15E+01 1 2 6.73 2.34E+04 2 2.79 66942 Potassium voltage-gated channel subfamily A member 5 (Voltage-gated potassium channel subunit Kv1.5) (HK2) (HPCN ACTB_CAVPO (Q71FK5) 4.22E+01 2 2 6.69 1.58E+04 0 7.82 41359 Actin, cytoplasmic 1 (Beta-act

TAF9_RAT (Q5BKE0) 2.94E+01 1 2 6.56 2.24E+04 2 0.00 28653 Transcription initiation factor TFIID subunit 9 (Transcription initiation factor TFIID 31 kDa subunit) (TAFII-31) (TAFII-32) (TAFII UCRH_MOUSE (P99028) 2.03E+01 1 2 6.51 1.56E+04 1 17.65 10080 Ubiquinol-cytochrome c reductase complex 11 kDa protein, mitochondrial precursor (EC 1.10.2.2) (Mitochondrial hinge protein) (Cytochrome C1, nonheme 11 kDa protein) (Complex III subunit VI

FPPS_MOUSE (Q920E5) 2.73E+01 1 2 6.34 2.15E+04 2 8.31 40142 Farnesyl pyrophosphate synthetase (FPP synthetase) (FPS) (Farnesyl diphosphate synthetase) (Cholesterol- regulated 39 kDa protein) (CR 39) [Includes: Dimethylallyltranstransferase (EC 2.5.1.1); Geranyltranstransferase (EC 2.5.1.1

NCKX6_MOUSE (Q925Q3) 4.59E+01 2 2 6.29 1.82E+04 2 7.75 64038 Sodium/potassium/calcium exchanger 6 precursor (Na(+)/K(+)/Ca(2+)-exchange protein 6) (Solute carrier family 24 member Differential expression of cardiac mitochondrial proteins

MYOG_MOUSE (P12979) 2.80E+01 1 3 6.19 1.51E+04 1 4.09 24862 Myogenin (MYOD1-related prote IDH3A_MOUSE (Q9D6R2) 2.49E+01 1 2 6.01 2.07E+04 1 4.70 39297 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor (EC 1.1.1.41) (Isocitric dehydrogenase) (NAD(+)- specific IC

FIBA_RAT (P06399) 3.81E+01 2 2 5.98 1.59E+04 2 6.68 86403 Fibrinogen alpha chain precursor [Contains: Fibrinopeptide MK10_MOUSE (Q61831) 4.72E+01 2 2 5.91 2.00E+04 2 10.65 52171 Mitogen-activated protein kinase 10 (EC 2.7.1.37) (Stress-activated protein kinase JNK3) (c-Jun N- terminal kinase 3) (MAP kinase p49 3F

RPC62_MOUSE (Q9D483) 4.35E+01 2 2 5.68 1.91E+04 1 6.62 60295 DNA-directed RNA polymerase III 62 kDa polypeptide (EC 2.7.7.6) (RNA polymerase C subunit 3) (RPC3) (RNA polymerase III C62 subun

ATP7A_CRIGR (P49015) 3.80E+01 2 2 5.68 1.67E+04 2 3.06 159985 Copper-transporting ATPase 1 (EC 3.6.3.4) (Copper pump 1) (Fragme SAFB2_MOUSE (Q80YR5) 3.68E+01 2 2 5.52 1.67E+04 2 3.75 111364 Scaffold attachment factor SAFB1_RAT (O88453) 4.62E+01 2 2 5.43 2.13E+04 2 5.50 104211 Scaffold attachment factor B (Scaffold attachment factor B1) (SAF RL27A_MOUSE (P14115) 2.25E+01 1 2 5.24 2.15E+04 0 9.79 16176 60S ribosomal protein L27a (L H2AO_HUMAN (P20670) 2.18E+01 1 2 5.22 2.09E+04 0 22.83 13837 Histone H2A.o (H2A/o) (H2A.2) (H2a-615 FTHFD_MOUSE (Q8R0Y6) 3.56E+01 2 2 5.15 1.73E+04 2 3.67 98271 10-formyltetrahydrofolate dehydrogenase (EC 1.5.1.6) (10-FTHFDH) (Aldehyde dehydrogenase 1 family member Differential expression of cardiac mitochondrial proteins

DYR2_HUMAN (Q92630) 1.95E+01 1 2 4.93 1.98E+04 2 4.56 59629 Dual-specificity tyrosine- phosphorylation regulated kinase 2 (EC 2.7.1.112) (EC 2.7.1.37 CYC2_MOUSE (P00015) 2.16E+01 1 2 4.66 2.32E+04 2 18.00 11308 Cytochrome c, testis-speci total 341 2069