Lithobius: a big genus of a small subphylum (Myriapoda), phylogeny and evolutionary history
- Project report – Anne-Sarah Ganske
[email protected] Natural History Museum Vienna, 3rd Zoological Department, Austria Myriapoda – the leggiest arthropods
o Symphyla o Pauropoda
o Diplopoda Chilopoda
Scutigeromorpha Lithobiomorpha Craterostigmomorpha Scolopendromorpha Geophilomorpha
Edgecombe)&)Giribet) 2002
430 genera with more than 3200 valid species 130 genera/subgenera with 1100 valid species
chilobase.biologia.unipd.it Zapparoli)&)Edgecombe)2011 all)photographs:)wikipedia.org Genus Lithobius Leach, 1814
Scutigerina6weberi
Scutigera6coleoptrata o > 500 species/subspecies
Pseudolithobius6megaloporus o species inter-relationships not clear Bothropolys6multidentatus
Eupolybothrus6fasciatus o non-monophyletic 80 Lithobius6(Lithobius)forficatus
Australobius6scabrior
93 Lithobius6(Ezembius))giganteus
Hessebius6plumatus Lithobius6forficatus6Linnaeus,)1758 Harpolithobius6anodus LITHOBIIDAE
Pleurolithobius6patriarchalis
Lithobius6(Sigibius) microps
Lithobius6(Monotarsobius) ho l sti i HENICOPIDAE 100 Anopsobius6neozelanicus
Dichelobius6bicuspis
83 Dzhungaria6gigantea
Zygethobius6pontis 84 Lamyctes6emarginatus
Henicops6washpoolensis
Paralamyctes6validus ©))Alex)Hyde
Koch)&)Edgecombe)2008 Systematics of genus Lithobius?
Morphological analysis Molecular analysis
Scutigerina6weberi
Scutigera6coleoptrata
Pseudolithobius6megaloporus
Bothropolys6multidentatus
Eupolybothrus6fasciatus
80 Lithobius6(Lithobius)forficatus
Line)drawings)of)Lithobius6forficatus6 Linnaeus,) 1758)(Rilling) 1960) Australobius6scabrior
93 Lithobius6(Ezembius))giganteus
Hessebius6plumatus
Harpolithobius6anodus LITHOBIIDAE
Pleurolithobius6patriarchalis
Lithobius6(Sigibius) microps
Lithobius6(Monotarsobius) ho l sti i HENICOPIDAE 100 Anopsobius6neozelanicus
Dichelobius6bicuspis
83 Dzhungaria6gigantea
Zygethobius6pontis 84 Lamyctes6emarginatus
Henicops6washpoolensis
Paralamyctes6validus
Line)drawings)of)Pseudolithoibus6megaloporus6 (Stuxberg,)1875))(Applegarth) 1952) Koch)&)Edgecombe)2008 Imaging methods
Multifocus)light[micrograph SEM[photograph Volume)rendering,)L. forficatus L. forficatus,6head,) ventral)view bristles) on)epipharynx voxel)size)15.3)µm Peristomatic structures - overview
35)species)of)lithobiid)genera: Lithobius6Leach,)1814) epipharynx) Neolithobius6Stuxberg,)1875) Disphaerobius6Attems,)1926) Eupolybothrus6 Verhoeff,)1907)
anterior Head)of)Lithobius6forficatus6(ventral)view) hypopharynx Volume)rendering,)voxel)size)5.0)µm Koch)&)Edgecombe)2008)(modified) Epipharynx - overview
ventral
labral)part
transverse)bulge
bottle[shaped)epidermal) gland)openings
clypeal)part 100)µm SEM,)L. pyrenaicus
Ganske)et)al.)(submitted) Transverse bulge
10)µm
L. (Lithobius))macilentus
20)µm L.)(Lithobius) lucifugus Ganske)et)al.)(submitted) Bottle-shaped epidermal gland openings
20)µm L.)(Lithobius) mutabilis
5)µm L.)(Lithobius) validus
Ganske)et)al.)(submitted) Peristomatic structures - overview
35)species)of)lithobiid)genera: Lithobius6Leach,)1814) epipharynx) Neolithobius6Stuxberg,)1875) Disphaerobius6Attems,)1926) Eupolybothrus6 Verhoeff,)1907)
anterior Head)of)Lithobius6forficatus6(ventral)view) hypopharynx Volume)rendering,)voxel)size)5.0)µm Koch)&)Edgecombe)2008)(modified) Hypopharynx - overview
mouth)opening ventral
spines)of)the)median)crest)margin
lips)forming)median)crest
100)µm
SEM,)L. forficatus Ganske)et)al.)(submitted) Spines)of)the)median)crest)margin
50)µm L.)(Lithobius) pelidnus
5)µm 10)µm L.)(Lithobius) muticus L.)(Sigibius) microps Ganske)et)al.)(submitted) Peristomatic structures – further characters…
Ganske)et)al.)(submitted) Peristomatic structures – conference contributions
8)peristomatic)characters)with)systematic/phylogenetic)potential
high)variability)of)microstructures
Talk at 17th InternationalCongress ofMyriapodology Krabi, Thailand
Ganske A[S, Edgecombe GD, Akkari N Exploring the peristomatic structures as a source of potential phylogenetic characters for the highly diverse genus Lithobius (Lithobiomorpha: Lithobiidae)
Talk at 4th InternationalCongress on InvertebrateMorphology Moscow, Russia
Ganske A[S, Edgecombe GD, Akkari N Using traditional and innovative imaging techniques to investigate internal cephalic structures in the highly diverse genus Lithobius (Chilopoda, Myriapoda) for phylogeny
Ganske A(S, Edgecombe GD, Akkari N (submitted) The peristomatic structures as a source of systematic characters in the genus Lithobius Leach, 1814 (Myriapoda, Chilopoda) ZooKeys Internal cephalic structures
HeadKofKLithobius)forficatus)(ventralKview) VolumeKrendering,KvoxelKsizeK5.0Kµm
Tentorium Mandible anterior Epipharynx Hypopharynx FirstKmaxillae Mandible - overview anterior
aciculae
gnathal)edge 180°
Tentorium Mandible Epipharynx Hypopharynx
teeth 100)µm
pulvillus SEM,)L. erythrocephalus Spines on)internal)side
absent
50)µm L.)(Lithobius) piceus
100)µm
L.)(Lithobius) fagei
50)µm L.)(Lithobius) electus
20)µm 10)µm Mandibular gnathal edge– further characters… Mandible – conference contribution
Poster at 17th InternationalCongress ofMyriapodology Krabi, Thailand
Ganske A[S, Edgecombe GD, Akkari N Investigation of the mandibular structures and evaluation of their phylogenetic significance in the genus Lithobius (Lithobiomorpha: Lithobiidae) Research activities 2017
Publications Submitted Ganske A[S, Edgecombe GE, Akkari N: The peristomatic structures as a source of systematic characters in the genus Lithobius Leach, 1814 (Myriapoda, Chilopoda), ZooKeys
Close to submission Akkari N, Ganske A[S, Komerički A, Metscher B: New avatars for Myriapods: 3D reconstructions of the holotype and the paratype as cybertypes for the species Eupolybothrus liburnicus Akkari, Komerički, Weigand, Edgecombe, Stoev 2017 (Chilopoda, Lithobiomorpha, Lithobiidae), PlosONE
In progress Exploring the mandibular structures and evaluation of their phylogenetic significance in the genus Lithobius (Lithobiomorpha: Lithobiidae)
Volume)rendering)with)highlighted)eggs Eupolybothrus6liburnicus6Akkari,)Komerički,)Weigand,)Edgecombe,)Stoev)2017 voxel) size)21)µm Research activities 2017
Conferences OralKpresentations Ganske A[S, Edgecombe GD, Akkari N: Using traditional and innovative imaging techniques to investigate internal cephalic structures in the highly diverse genus Lithobius (Chilopoda, Myriapoda) for phylogeny (ICIM4, Moscow; August) Ganske A[S, Edgecombe GD, Akkari N: Exploring the peristomatic structures as a source of potential phylogenetic characters for the highly diverse genus Lithobius (Lithobiomorpha: Lithobiidae) (17thICM, Krabi; July) Poster presentation Ganske A[S, Edgecombe GD, Akkari N: Investigation of the mandibular structures and evaluation of their phylogenetic significance in the genus Lithobius (Lithobiomorpha: Lithobiidae) (17thICM, Krabi; July) Research activities 2017
Workshops & Courses BIG4[Workshop: Morphology of Invertebrates – 3D Imaging and Novel Approaches for Biosystematics, NHM[Wien, Austria (May) Molecular Phylogenetics Course, Cambridge University, UK (April) EU ABS Regulation Training Workshop, Budapest, Hungary (February)
MuseumsKvisits
The)Natural)History)Museum,)London,) UK)(April)
Hungarian)Natural)History)Museum,)Budapest)(February)
FieldKtrips
Collecting)trip,)La)Palma,)Spain)(October)
Collecting)trip)(Myriapoda),)Carinthia,)Austria)(June) Upcoming research activities
Conferences & Events Oral presentation Ganske A[S, Akkari N: On the significance of morphological characters of head structures for the systematics of the centipede genus Lithobius (Myriapoda) (NOBIS Young Researchers’ Day, Vienna, Austria) (November 2017) Poster presentation Ganske A[S: Klein, kleiner, mikro – filigrane Strukturen in den Köpfen der Steinläufer (Carl von Schreibers Forschungspreis, Vienna, Austria) (December 2017)
Secondment Research stay at the University of Turku, Molecular analysis of genus Lithobius (spring 2018)
Others Departmental Seminar Integrative Zoology, University of Vienna, Austria Soon enrolled as PhD[student at University of Vienna, Integrative Zoology, Prof. Dr. Andreas Wanninger This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 642241.