Florida Bonneted Bat Acoustic Survey Kaicasa

Kaicasa

Protected Species Survey

March 2005 Revised September 2005 Revised February 2008 Revised August 6, 2018 Revised June 4, 2020

Prepared for:

Habitat for Humanity of Collier County 11145 Tamiami Trail East Naples, Florida 34113

Prepared by:

2122 Johnson Street Fort Myers, Florida 33901

TABLE OF CONTENTS

Page

TABLE OF CONTENTS

1.0 INTRODUCTION ...... 1 2.0 SURVEY METHODOLOGY ...... 1 3.0 SITE CONDITIONS ...... 3 4.0 SURVEY RESULTS AND DISCUSSION 4.1 Permit Research ...... 6 4.2 Onsite Survey ...... 9 5.0 WILDLIFE MANAGEMENT PLANS ...... 13 6.0 REFERENCES ...... 20

LIST OF TABLES: TABLES:

Table 2-1 Protected Species Survey Conditions ...... 3 Table 4-1 Observed Species, June 2020 ...... 9 Table 4-2 Potential Listed Species ...... 10

LIST OF FIGURES: FIGURES:

Figure 1 Project Location Map ...... 2 Figure 2 Protected Species Map with FLUCFCS ...... 12

LIST OF APPENDICES

Appendix A: Florida Bonneted Bat Acoustic and Visual Inspection Surveys

1.0 INTRODUCTION

Johnson Engineering ecologists conducted an updated Protected Species Survey (PSS) per Florida Fish and Wildlife Conservation Commission (FWC) and U.S. Fish and Wildlife Service (FWS) guidelines on the Habitat for Humanity of Collier County (HHCC) 100-acre Kaicasa project. The site is located in Section 12, Township 47 South, Range 29 East, north of State Road 29, and east of Farm Worker Village.

The project area is bound by a residential neighborhood known as Farm Worker Village to the west, State Road 29 and an active orange grove to the south, and to the north and east by undeveloped land and rangeland. This PSS was performed and updated to determine the extent of use by state and federal listed species on the subject property. Please refer to the Project Location Map (Figure 1).

2.0 SURVEY METHODOLOGY

Cover and vegetation association types were delineated utilizing 2003 digital aerial photography, USDA SCS soil survey maps for Collier County (USDA 1998), and onsite field investigations. The cover and vegetation assemblages were classified based on the nomenclature of the Florida Land Use Cover and Forms Classification System, Levels III and IV (FLUCFCS) (Florida Department of Transportation, 1999). This system, originally developed by the Florida Department of Transportation (FDOT), allows for a uniform but flexible means of classifying land uses important for determining the presence of wetlands and suitable habitat for protected species. Please see the FLUCFCS map and attached protected species locations.

The original PSS was conducted on March 4th and 7th, 2005. A site visit was also conducted on September 22, 2005 with Collier County staff, a panther prey survey was conducted April 9-10; and 13-15, 2007. Updated PSSs were conducted on January 22, 2008, July 26-30, 2018, and June 3, 2020. Acoustic surveys for the Florida bonneted bat (FBB) were conducted in September 2015 and June 2017. An FBB roost survey was conducted November 2015. Table 2-1 provides weather conditions during the surveys and site visits.

-1- PROJECT LOCATION

LEE COUNTY

HENDRY COUNTY BROWARD COUNTY DADE COUNTY

MONROE COUNTY

PROJECT LOCATION

LOCATION MAP

Table 2-1: Protected Species Survey and Site Visit Conditions

Date Time Began Time Ended Cloud Cover Temperature Wind 03/04/05 9:15 AM 3:45 PM Partly Cloudy Mid 60s to Low 70s South 0-5 mph 03/07/05 10:15 AM 3:30 PM Partly Cloudy Mid 60s to Low 70s South 10-15 mph 9/22/05 10:00 AM 12:00 PM Clear Mid 80s East 10-15 mph 4/9/07 9:00 AM 12:00 PM Clear Mid 70’s North 5-10 mph 1/22/08 11:00 AM 3:30 PM Clear Mid 70’s East 5-10 mph 9/24/15 Acoustic N/A Cloudy Mid 80’s NE 0-5 mph 10/2/15 Acoustic N/A Partly cloudy Mid 80’s NW 0-5 mph 11/11/15 Snag Peep N/A Cloudy Upper 70’s NE 3-7 mph 6/9/17 Acoustic N/A Cloudy Low 80’s NE 0-5 mph 6/11/17 Acoustic N/A Cloudy Mid 80’s E 5-10 mph 7/26/18 10:00 AM 1:00 PM Partly Cloudy Upper 80’s SSW 5-10 mph 7/27/18 10:00AM 12:15 PM Mostly Cloudy Low 90’s SW 0-5 mph 7/30/18 10:30 AM 1:00 PM Partly Cloudy Low to mid 80’s SW 10-15 mph 6/3/20 8:00 AM 3:00 PM Mostly Cloudy Mid 70’s to Low 80’s E 5-10 mph

The surveys were conducted utilizing the “Meandering Strip Census” methodology. A series of parallel transects were conducted in the field spaced approximately fifty (50) feet apart in width. Transects were designed to achieve a minimum eighty (80) percent survey coverage. Each FLUCFCS habitat was surveyed for signs of protected species.

3.0 SITE CONDITIONS

The subject property includes 12 vegetative communities which were surveyed for the presence of listed species. A discussion of habitat types delineated by FLUCFCS code on the Kaicasa site during the PSS with dominant vegetation species noted is provided below:

FLUCFCS Code 211, Improved Pasture (10.48 acres)

This upland community is located in the northern portion of the site. The canopy and midstory is open but includes scattered slash pine (Pinus elliottii), cabbage palm (Sabal palmetto), and wax myrtle (Morella cerifera). Herbaceous species include Bahia grass (Paspalum notatum), common ragweed (Ambrosia artemisiifolia), saw palmetto (Serenoa repens), smutgrass (Sporobolus indicus), black-root (Pterocaulon virgatum), caesarweed (Urena lobata), and runner oak (Quercus elliottii).

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FLUCFCS Code 212, Unimproved Pasture (9.74 acres) The canopy of this upland community includes scattered slash pines. Sub-canopy includes cabbage palms and wax myrtle. Herbaceous cover is similar to that of the open rangeland with Bahia grass the dominant species. Other herbaceous species include saw palmetto, paw paw (Asimina reticulata), and smutgrass.

FLUCFCS Code 411, Pine Flatwoods, (30.44 acres) Upper canopy in this coverage area includes slash pine and scattered live oak. Mid-canopy is dominated by saw palmetto, cabbage palm, rusty lyonia (Lyonia ferruginea), and American beautyberry (Calicarpa americana). Herbaceous cover includes saw palmetto, broomsedge (Andropogon virginicus), gallberry (Ilex glabra), grapevine (Vitis rotundifolia), and wild blueberry (Vaccinium myrsinites).

FLUCFCS Code 427/428, Live Oak-Cabbage Palm (1.59 acres) This upland area includes live oak (Quercus virginiana), and cabbage palm in the canopy. Midstory vegetation includes cabbage palm, wax myrtle, and Brazilian pepper (Schinus terebinthifolius). Herbaceous vegetation includes caesarweed, Bahia grass, and poison ivy (Toxicodendron radicans).

FLUCFCS Code 434, Mixed Hardwood Conifer Forest (15.92 acres) This upland area includes slash pine, live oak, red maple (Acer rubrum), and cabbage palm in the canopy. Mid story vegetation includes cabbage palm, American beautyberry, and Brazilian pepper. Herbaceous vegetation includes saw palmetto, American beautyberry, and caesarweed.

FLUCFCS Code 514, Canal (2.32 acres) This community includes the large canal adjacent to State Road 29. The canopy in this area is absent. Mid-story vegetation includes Brazilian pepper and scattered cabbage palm. Herbaceous vegetation includes arrowhead (Sagittaria lancifolia), floating hearts (Nymphoides aquatica), and maidencane (Panicum hemitomon).

FLUCFCS Code 617, Mixed Wetland Hardwoods (0.09 acres) Canopy vegetation within this wetland community includes laurel oak (Quercus laurifolia) and red maple. Mid-canopy species include Brazilian pepper, cabbage palm, and groundsel tree (Baccharis glomeruliflora). Herbaceous vegetation includes pennywort (Hydrocotyle umbellata),

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Asiatic pennywort (Centella asiatica), and swamp fern (Blechnum serrulatum) along the edges of the canal.

FLUCFCS Code 625, Hydric Pine Flatwoods (1.72 acres) This transitional wetland community includes slash pine and cabbage palm in the canopy. Midstory vegetation is predominantly wax myrtle. Herbaceous species include yellow-eyed grass (Xyris spp.), beakrush (Rhynchospora spp.), rosy camphorweed (Pluchea rosea), and sand cordgrass (Spartina bakeri).

FLUCFCS Code 641, Freshwater Marsh (8.93 acres) The canopy and sub-canopy of this wetland community is open. Herbaceous species include pickerelweed (Pontederia cordata), arrowhead, pennywort, and cordgrass.

FLUCFCS Code 643, Wet Prairie (17.20 acres) The canopy of this wetland community is open but includes widely scattered slash pine. Mid- canopy vegetation includes scattered wax myrtle. Herbaceous species include blue maidencane (Amphicarpum muhlenbergianum), beakrush, yellow-eyed grass, and saw grass (Cladium jamaicense).

FLUCFCS Code 740, Disturbed Land (1.25 acres) This disturbed upland community is open in the canopy and mid-canopy strata. Herbaceous species include bahia grass, ragweed, dog fennel, and false buttonweed (Spermacoce verticillata).

FLUCFCS Code 7401, Disturbed Land, Hydric (0.32 acres) This wetland community does not include canopy vegetation. Mid-story vegetation includes wax myrtle and groundsel tree. Herbaceous species include yellow-eyed grass, beakrush, and blue maidencane.

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4.0 SURVEY RESULTS AND DISCUSSION

4.1 Permit Research

The Kaicasa project has been reviewed for potential impacts to state and federal listed species through Section 7 of the Endangered Species Act (ESA) by FWS and FWC. Research into the potential utilization of the Kaicasa project by certain listed species was performed and reviewed during the permitting processes. Specific research and findings regarding potential impacts to wood storks (Mycteria americana) and other listed wading birds, American bald eagle (Haliaeetus leucocephalus), Florida panther (Felis concolor coryi) and Florida bonneted bat (Eumops floridanus) are provided below.

Wood Stork:

Wood storks are listed as endangered by both the U.S. Fish and Wildlife Service (FWS) and the Florida Fish and Wildlife Conservation Commission (FWC). The subject parcel is located approximately 13 miles east of the Corkscrew Swamp Sanctuary, a known wood stork rookery and is within the 18.6-mile Core Foraging Area as designated by FWS. Johnson Engineering provided FWS with an evaluation and comparison of wood stork foraging prey biomass, availability and consumption based on hydroperiods of the impact sites and proposed compensation sites during the federal permitting process. The analysis showed that the combination of onsite improvements and improvements through credits purchased from the Big Cypress Mitigation Bank will adequately compensate for onsite wood stork foraging losses. The FWS concurred with the determination that the project “may affect but is not likely to adversely affect” the wood stork.

American Bald Eagle:

The American bald eagle was delisted on August 9, 2007 and is currently protected by the Bald and Golden Eagle Protection Act and the Migratory Bird Treaty Act. Research into the location of known bald eagle nests in the vicinity of the Kaicasa project was conducted through the utilization of FWC's Internet based bald eagle nest locator. A query for nests located within a five-mile radius of the parcel revealed three nests. Bald

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eagle nest number CO 021 is located approximately one mile north-northeast of the site. Bald eagle nest number CO 034 is located approximately two miles north of the site, and nest number CO 025 is located approximately four miles south-southeast of site. These distances exceed any protection measures in effect. Therefore, no adverse impacts to eagles are anticipated.

Florida Panther:

The Florida panther is listed as endangered by FWS. The Kaicasa project is located within Primary Zone habitat as denoted by the Florida Panther MERIT Group. FWS required formal consultation under Section 7 of the ESA in a letter on July 24, 2007 for Kaicasa. Subsequently, a biological assessment including a breakdown of habitat impacts, telemetry data, panther prey analysis, analysis of adjacent and nearby lands, traffic analysis, and other information necessary was submitted to the FWS on August 28, 2007. Mitigation for impacts to panthers was negotiated during the FWS formal consultation. In 2008 FWS issued a biological opinion determining that the project’s impacts to 71.9 acres equated to the loss of 1,465.50 panther habitat units (PHU). The applicant purchased 3.81 wetland credits (equating to 34.13 PHU) from the Big Cypress Mitigation Bank and 1,431.37 PHU from the Florida Panther Conservation Bank to provide the mitigation deemed necessary by FWS.

The FWS Habitat Assessment Methodology for the Florida Panther was updated in 2009, and a recalculation of compensation for impacts to panther habitat was deemed appropriate by FWS biologists. Using the most recent habitat assessment scores and updated plans for the Kaicasa project, the recommended compensation for project-related impacts to panther habitat was calculated by FWS as 1,313 PHUs, which is 152 PHUs less than HHCC purchased to compensate for impacts associated with the Kaicasa project. FWS determined that the excess 152 PHUs purchased for the Kaicasa project could be applied by HHCC to future projects that require panther compensation.

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Florida Bonneted Bat:

The Kaicasa project is located within the FBB Consultation Area (FWS 2013). Proposed Project activities would convert 54.6 ac of FBB habitat to urban landscape. Five on-site lakes totaling 13.25 acres and four on-site preserve areas totaling 32.1 acres would provide continued FBB foraging opportunities. A total of 14 cavity trees suitable for FBB were identified during the Consultant’s roost survey, five of which are located within preserve areas and will remain intact after land clearing. All potential roost cavities on the Project site were examined by video peep or cavity-directed acoustic surveys. No evidence of FBB roosting was detected during the surveys. HHCC has agreed to implement conservation measures or Project commitments in coordination with FWS to minimize adverse effects to the FBB as a result of construction activities associated with the Kaicasa project. These commitments specifically include the inspection of potential roosts prior to removal to determine if any undocumented FBB roosts are present. Roost surveys will be conducted by a professional environmental consultant with expertise in detecting FBB roost sites using a video probe (peeper) to observe the cavity contents, including top and bottom portions of the cavity. Additional acoustic surveys may be required if cavity peeping is not possible for any cavity, or the entire cavity cannot be observed, or the contents cannot be identified. A written report of the survey dates, personnel, equipment, and survey results will be provided to FWS prior to initation of construction activities. Additionally, the purchase of mitigaion credits from Florida Panther Conservation Bank (FPCB) and Big Cypress Mitigation Bank (BCMB) has preserved approximately 168 ac of FBB foraging and potential roosting habitat, an amount equal to more than three times the Kaicasa project’s impacts.

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4.2 Onsite Survey

Prior to conducting onsite investigations, a comprehensive list of potential protected species was assembled. This list includes each habitat type observed onsite and all potential listed species known to occupy similar habitats. Additionally, a list of species observed during the June 2020 survey is provided in Table 4-1. Table 4-2 lists potential protected species by FLUCFCS Code.

The onsite species survey was conducted by traversing parallel transects through each habitat type while observing the terrain for signs of listed species. Transects were spaced to provide a minimum of 30% visual coverage of each community. Upland habitats were observed for signs of gopher tortoise, indigo snakes, bats, fox squirrels, bear, panther, and other listed species indicative of such communities. One gopher tortoise burrow was observed in the previous PSS conducted in 2008 but was not located during the 2018 survey. Subsequently a new Potentially Occupied gopher tortoise burrow was located during the 2018 survey. Both burrow locations are delineated on the attached Protected Species Map (Figure 3).

Wetlands on the Kaicasa site were observed primarily for the presence of listed wading bird species. During a September 22nd and July 2018 field investigation, several species of listed wading birds were observed utilizing the onsite wetlands, however no signs of roost or nesting activities were observed. Tables 4-1 and 4-2 delineate listed species observed during the survey.

Table 4-1. Observed Species: June 2020

Common Name Scientific Name Cattle egret Bubulcus ibis Red shouldered hawk Buteo Lineatus Northern cardinal Cardinalis cardinalis Turkey Vulture Cathartes aura Ground dove Columbina passerina Blue jay Cyanocitta cristata Red-bellied woodpecker Melanerpes carolinus Eastern cottontail Sylvilagus floridanus White-eyed vireo Vireo griseus Mourning dove Zenaida macroura

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Table 4-2. Potential Listed Species

FLUCFCS Common Name Scientific Name Observed (Y/N) 211 Gopher Frog Rana Capito N 212 Eastern Indigo Snake Drymarchon corais couperi N Gopher Tortoise Gopherus Polyphemus N Florida Sandhill Crane Grus canadensis pratensis N Whooping Crane Grus americana N Burrowing Owl Athene cunicularia N Florida Panther Felis concolor coryi N Florida Black Bear Ursus americanus floridanus N

411 Gopher Frog Rana Capito N Eastern Indigo Snake Drymarchon corais couperi N Gopher Tortoise Gopherus Polyphemus N Bald Eagle Haliaeetus leucocephalus N Florida Panther Felis concolor coryi N Florida Black Bear Ursus americanus floridanus N Big Cypress Fox Squirrel Sciurus niger avicennia N

427/428 Eastern Indigo Snake Drymarchon corais couperi N Bald Eagle Haliaeetus leucocephalus N Florida Panther Felis concolor coryi N Florida Black Bear Ursus americanus floridanus N Big Cypress Fox Squirrel Sciurus niger avicennia N

434 Eastern Indigo Snake Drymarchon corais couperi N Bald Eagle Haliaeetus leucocephalus N Florida Panther Felis concolor coryi N Florida Black Bear Ursus americanus floridanus N Gopher Tortoise Gopherus polyphemus Y

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Table 4-1. Potential Listed Species; Continued Observed FLUCFCS Common Name Scientific Name (Y/N) 512 American Alligator Alligator mississippiensis N Reddish Egret Egretta rufescens N Little Blue Heron Egretta caerulea N Tricolored Heron Egretta tricolor N Florida Sandhill Crane Grus canadensis pratensis N Whooping Crane Grus americana N Wood Stork Mycteria americana N Roseate Spoonbill Ajaja ajaia N

617 Reddish Egret Egretta rufescens N 625 Little Blue Heron Egretta caerulea N 641 Tricolored Heron Egretta tricolor N 643 Florida Sandhill Crane Grus canadensis pratensis N 7401 Whooping Crane Grus americana N Wood Stork Mycteria americana N Roseate Spoonbill Platalea ajaja N

740 Gopher Frog Rana Capito N Eastern Indigo Snake Drymarchon corais couperi N Gopher Tortoise Gopherus Polyphemus N Florida Sandhill Crane Grus canadensis pratensis N Whooping Crane Grus americana N Burrowing Owl Athene cunicularia N Florida Panther Felis concolor coryi N Florida Black Bear Ursus americanus floridanus N

-11- 211 0.38 AC 212 0.80 AC 434 6.44 AC

211 10.10 AC

641 LEGEND 7401 0.75 AC 0.25 AC

641 434 643 0.44 AC 0.31 AC 3.26 AC 411 643 0.92 AC 1.79 AC

625 641 0.70 AC 0.58 AC

411 643 12.16 AC 1.85 AC

427/428 1.59 AC 212 8.74 AC

641 2.49 AC

625 643 0.70 AC 10.30 AC 641 0.92 AC

411 11.96 AC

641 625 2.24 AC 0.32 AC 740 7401 1.25 AC 0.07 AC

411 5.40 AC GT-1-AB GT-2-AB 641 0.24 AC Old GT

434 2.32514 AC 641 9.16 AC 0.99 AC

617 0.09 AC

641 0.28 AC

KAICASA HABITAT FOR FLUCFCS WITH GOPHER TORTOISE LOCATION HUMANITY

5.0 WILDLIFE MANAGEMENT PLANS

No signs of federal or state listed species were observed on the Kaicasa property during the March 2005 Protected Species Survey. However, during the panther prey survey conducted in April 2007 tracks of an adult panther and young were documented and confirmed by Daryl Land, FWC. In addition, during the January 2008 survey a gopher tortoise burrow was located in the southern portion of the site. This gopher tortoise burrow was not located during the June 2020 survey, but two other potential gopher tortoise burrows were observed. A limpkin (Aramus guarauna) was observed during the July 2018 survey. The limpkin was delisted on January 11, 2017 but is still included in the Imperiled Species Management Plan.

Gopher Tortoise Management Plan During the July 2018 protected species survey, one potentially occupied gopher tortoise burrow was identified within the southern portion of the Kaicasa project. During the June 2020 update, that burrow and one other burrow were observed as shown in Figure 2 in an area proposed for development. Both burrows show signs of use by armadillos including loose dirt, and mushrooms and plants growing in the burrow mouth. In accordance with the January 2017 Gopher Tortoise Permitting Guidelines (Guidelines) published by the FWC, a 100% Gopher Tortoise survey will be conducted over suitable gopher tortoise habitat occurring within 25 feet of proposed development by an Authorized Gopher Tortoise Agent (Agent) prior to site clearing or construction activities. All potentially occupied and abandoned burrows will be marked in the field and GPS located. If ten or fewer gopher tortoise burrows are identified in the 100% survey, an application for a ten an offsite 10 or fewer burrows relocation permit will be submitted to FWC. If more than ten burrows are identified, then a Conservation Permit will be required from FWC. Upon issuance of the relocation/conservation permit from FWC and a vegetation removal permit from Collier County all potentially occupied gopher tortoise burrows will be excavated in accordance with the Guidelines using a combination of mechanical and hand removal methods. Mechanical excavation will be performed using a rubber tired or rubber tracked excavator with a smooth- edged bucket and supervised by an Agent. Hand excavation will be employed to clear the burrow and ensure the mechanical excavator remains a safe distance from any tortoise(s) within the

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Wading Birds FLUCFCS Codes 512 (Canal), 617 (Mixed Wetland Hardwoods), 625 (Hydric Pine Flatwoods), 641 (Freshwater Marsh), and 643 (Wet Prairie) all provide suitable foraging habitat for wading birds on the Kaicasa project site. Little blue herons, snowy egrets, limpkin and white ibis have been observed foraging in wetlands during onsite investigations. In addition, Kaicasa is located approximately 13 miles southeast of the Corkscrew Swamp Sanctuary, a known wood stork rookery. The lack of forested wetland communities onsite limit potential utilization of Kaicasa by wading birds for roosting, but its proximity to the Corkscrew Swamp Sanctuary means the site is within the core foraging area for wood storks. Continued maintenance of the hydrology and vegetation within retained wetlands in combination with proposed water management features will provide foraging opportunities for wood storks and other wading birds throughout the year. Preserve areas will also include upland connections between wetlands. Hydraulic connections between wet and dry storm water management areas and the preserved wetlands will be designed to maintain a healthy hydroperiod. This will continue to provide well-established foraging locations over a wide range of seasonal water levels. Additionally, all surface water management lakes will include littoral plantings, which provide forage and cover to small fish, macroinvertebrates, and other forage species. The lakes and wetlands will be maintained free of invasive exotic vegetation providing perpetual habitat for various wading bird species.

Florida Bonneted Bat Management Plan

Effective November 2, 2013, the US Fish and Wildlife Service (FWS) listed the Florida bonneted bat (Eumops floridanus) (FBB) as endangered under the Endangered Species Act (ESA). The Kaicasa development is located within the FWS Consultation Area for the FBB. FBBs are known to roost in both natural and artificial structures, including tree cavities, foliage, rock crevices, buildings, bridges and bat houses.

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Two acoustic FBB surveys were conducted in September 2015 and June 2017. Additionally, one roost survey was conducted in November 2015 to determine the potential usage of the Kaicasa site for roosting activities by FBB. Although the acoustic surveys showed the property may be utilized for foraging by FBB, no signs of roosting were observed.

The FWS issued an amended Biological Opinion (Service Consultation Code 41420-2007-F- 0491-R001) (BO) for the Kaicasa project on September 24, 2018, which includes conservation measures and recommendations to minimize adverse effects to the FBB as a result of construction activities associated with the project as descried below.

Onsite Mitigation

The FWS developed the Florida Bonneted Bat Consultation Guidelines in October 2019 that provide Best Management Practices (BMPs) designed to reduce impacts to FBB caused by developments. The draft BMPs suggest preserving 0.25 acres of native vegetation per every five acres of impacts, which calculates to preserving 5% of the impacted FBB habitat as native vegetation. As stated in the FWS BO, the Kaicasa project proposes to retain four onsite preserve areas totaling 32.10 acres that will provide continued FBB foraging and roosting opportunities. This equates to the preservation of over 30% of the site in its natural state. Additionally, the applicant proposes five onsite water management lakes totaling 13.25 acres, which could also be utilized by FBB for foraging. This exceeds the preservation requirements of any BMPs set forth by the FWS.

Off Site Mitigation

To offset impacts to wetlands and protected species associated with the proposed development of the Kaicasa project, the applicant requested to purchase offsite mitigation credits from the Florida Panther Conservation Bank and the Big Cypress Mitigation Bank. The purchase of mitigation credits has preserved approximately 168 acres of FBB foraging and potential roosting habitat, which exceeds the FWS BMPs.

Pre-Construction Roost Survey

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Acoustic surveys revealed that FBB may utilize the site for foraging. To ensure that potential future FBB roosts are not damaged during land clearing activities, the applicant will conduct a preconstruction FBB roost survey in accordance with FWS guidelines. The roost survey will be conducted by a qualified environmental professional and will occur no more than 90 days prior to initiation of clearing activities to ensure the most accurate and up-to-date information is used to determine the potential for FBB roosts within the project area. Cavity peeping will be performed using a video probe to observe the contents including the top and bottom portions of each cavity. The applicant will provide a written report of the survey dates, personnel, equipment and survey results to FWS. In the event that a FBB roost is detected, the applicant will immediately cease construction and consult with FWS to determine an appropriate buffer or other appropriate conservation effort.

These conservation measures were approved by FWS and will be implemented to ensure potential impacts to FBB are avoided to the greatest extent practicable.

Eastern Indigo Snake

The standard protection measures for the eastern indigo snake will be implemented during all clearing and construction phases of the project. Educational materials will be provided for all construction crews to follow. Educational materials will include pamphlets and a 24-inch by 36- inch poster that shall be conspicuously hung at the construction entrance for the project. The pamphlets and poster will include the following information: a. A description of the eastern indigo snake, its habits, and protection under Federal Law; b. Instructions not to harm, injure, harass or kill this species; c. Directions to notify the qualified biologist or designated leader if a snake is sighted; d. Directions to cease construction activity, notify the qualified biologist, and allow the snake sufficient time to move away from the site on its own before resuming construction; e. Telephone numbers of pertinent agencies to be contacted if a dead eastern indigo snake is encountered.

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Florida Black Bear

Although no signs of Florida black bear were observed during any of the surveys or site visits on the property, potential black bear encounters are possible. Kaicasa is bound to the north and east by large agricultural and undeveloped areas. Theses areas, and lands adjacent to them, are within the range of reported bear sightings. The following measures will be taken in order to protect the black bear:

a. Informational wildlife alert signs will be posted and maintained by the developer and/or Home Owners Association in perpetuity. This signage will inform residents and guests of the potential for bear encounters.

b. The developer and/or Home Owners Association shall be responsible for insuring that all residences shall be provided with wildlife proof garbage can enclosures. Garbage cans are to be stored within a secured structure until the morning before garbage collection.

c. The developer and/or Home Owners Association will be responsible to ensure that all residents shall keep all pets indoors or within a fenced yard, unless it is on a leash under the control of the owner. Dogs, cats and other domestic animals will not be permitted to roam freely within the development or any of the preserves. d. Should a bear be encountered within the development, it should be left undisturbed so that it can continue to its destination. However, if a bear continues to remain in the development for several hours, the Florida Game and Fresh Water Fish Commission will be contacted so that they can provide appropriate action to assist the bear out of the development. Under no condition is the bear to be disturbed, harmed, molested or fed. e. Bear relocation will only be used as a last resort if the bear continues to return and become a nuisance or threat to the residents. All bear encounters are not considered a nuisance or a threat, unless they remain in the area for long periods of time and continue to return over several days. The Florida Game and Fresh Water Fish Commission is responsible for determining the status of the bear and what action is necessary.

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Florida Panther

The USFWS required formal consultation under Section 7 of the Endangered Species Act of in a letter dated July 24, 2007 for Kaicasa. Subsequently, a biological assessment including a breakdown of habitat impacts, telemetry data, panther prey analysis, analysis of adjacent and nearby lands, traffic analysis, and other information was submitted to the FWS on August 28, 2007. During the panther prey survey Florida panther tracks and an adult with young were observed on August 9, 2007. Therefore, a Florida panther management plan is being provided. The management plan includes off-site mitigation, preserve management, and homeowner education.

The mitigation requirements are determined through coordination with the FWS and have been finalized. The FWS issued a Biological Opinion on July 2, 2008. At the time the Biological Opinion was issued, 71.9 acres of panther habitat were proposed to be impacted. The compensation required by the USFWS to offset these impacts was 1,465.50 panther habitat units. The mitigation proposed by the applicant included 3.81 wetland credits, equating to 34.14 panther habitat units, from Big Cypress Mitigation Bank and 1,431.36 panther habitat units from Florida Panther Conservation Bank. The panther habitat units from the Florida Panther Conservation Bank were purchased on July 23, 2008. The wetland credits are currently reserved. However, following the issuance of the Biological Opinion the project site plan was altered which decreased impacts to 70.7 acres, requiring 1,444.00 panther habitat units to offset the project impacts (Table 2). In addition, the wetland mitigation decreased to 2.85 credits, equating to 25.54 panther habitat units. Therefore, the applicant provided an excess of 12.9 panther habitat units to offset the projects impacts.

The site plan was designed to provide large, contiguous preserves which remain open to the east and therefore provide habitat for the endangered Florida panther and other wildlife as requested by Collier County and the FWS. All exotic / nuisance species identified by the Florida Exotic Pest Plant Council’s current list of most invasive plant species, and incorporated herein by reference, shall be removed by hand from the preservation areas. Maintenance will be conducted frequently enough to limit exotic/nuisance species coverage to no more than 5% total vegetative cover within the preserves. At a minimum, maintenance shall be conducted semi-annually (twice a year) for the first two years and once a year for the subsequent 3 years. Maintenance efforts

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will be increase if it appears the mitigation areas are in danger of not meeting success criteria. The maintenance program will be conducted in perpetuity.

Management will also include actions to educate residents on measures that are necessary for living in areas that Florida panthers utilize. The following measures will be taken to protect the Florida panther and limit panther/human interactions:

1. Informational wildlife alert signs will be posted and maintained by the developer and/or Home Owners Association in perpetuity. This signage will inform residents and guests of the potential for Florida panther encounters.

2. The developer and/or Home Owners Association will be responsible to insure that all residents shall keep all pets indoors or within a fenced yard, unless it is on a leash under the control of the owner. Dogs, cats and other domestic animals will not be permitted to range freely within the development or any of the preserves.

3. Should a Florida panther be encountered within the development, it should be left undisturbed so that it can continue to its destination. However, if a panther continues to remain in the development for several hours, the FFWCC will be contacted so that they can provide appropriate action to assist the panther out of the development. Under no condition is the panther to be disturbed, harmed, molested or fed.

4. Upon locating a dead, injured, or sick threatened or endangered species, initial notification must be made to the nearest Service Law Enforcement Office; Fish and Wildlife Service; 9549 Koger Boulevard, Suite 111; St. Petersburg, Florida 33702; 727- 570-5398. Secondary notification should be made to the FWC; South Region; 3900 Drane Field Road; Lakeland, Florida; 33811-1299; 1-800-282-8002.

5. Care should be taken in handling sick or injured specimens to ensure effective treatment and care or in the handling of dead specimens to preserve biological material in the best possible state for later analysis as to the cause of death. In conjunction with the care of sick or injured panthers or preservation of biological materials from a dead animal, the finder has the responsibility to carry out instructions provided by Law Enforcement to ensure that evidence intrinsic to the specimen is not unnecessarily disturbed.

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6.0 REFERENCES

Florida Department of Transportation, State Topographic Bureau, Thematic Mapping Section, 1999. Florida Land Use, Cover and Forms Classification System. Procedure No. 550- 010-001-a.

Florida Fish and Wildlife Conservation Commission Eagle Nest Locator, https://public.myfwc.com/FWRI/EagleNests/nestlocator.aspx Site Accessed 8/14/2018

Florida Fish and Wildlife Conservation Commission, 2017; Florida’s Endangered Species, Threatened Species and Species of Special Concern

Florida Fish and Wildlife Conservation Commission. 2017. Gopher Tortoise Permitting Guidelines. Tallahassee, Florida. 138pp.

Natural Resources Conservation Service. Soil Survey of Lee County, Florida. U.S. Department of Agriculture, Washington, D.C. http://www.nrcs.usda.gov/Internet/FSE_MANUSCRIPTS/florida/FL071/0/Lee.pdf Site accessed August 14, 2018

U. S. Fish and Wildlife Service, May 2007. National Bald Eagle Management Guidelines. 23 pp.

U.S. Fish and Wildlife Service Information for Planning and Conservation, Environmental Conservation Online System. 2016. http://ecos.fws.gov/ipac/gettingStarted/index. Site accessed August 14, 2018

U.S. Fish and Wildlife Service, 1999; Florida Panther Consultation Area Map

U.S. Fish and Wildlife Service, October 2019. Florida Bonneted Bat Consultation Guidelines. Vero Beach, FL. 29 pp.

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Florida Bonneted Bat Acoustic Surveys and Cavity Tree/Snag Inspections

Kaicasa

Conducted September - November 2015

Prepared for:

HABITAT FOR HUMANITY OF COLLIER COUNTY 11145 Tamiami Trail East Naples, FL 34113

Prepared by:

2122 Johnson Street Fort Myers, Florida 33901 (239) 334-0046

JEI# 20149937-000

TABLE OF CONTENTS

Page

1.0 INTRODUCTION ...... 1

2.0 METHODOLOGY 2.1 Acoustic Survey ...... 4 2.2 Acoustic Data Analysis ...... 7 2.3 Roost Cavity Survey ...... 7

3.0 RESULTS AND DISCUSSION 3.1 Acoustic Survey Results ...... 9 3.2 Roost Cavity Survey Results ...... 11 3.3 Effect Determination ...... 13 3.4 Conservation Measures to Minimize Impacts ...... 13

4.0 CONCLUSION ...... 14

5.0 REFFERENCES ...... 15

LIST OF FIGURES AND TABLES

Figure 1 Location Map ...... 3 Figure 2 Acoustic Survey Sites and Snag Locations ...... 6

Table 1 Acoustic Survey Results Summary Table ...... 10 Table 2 Cavity Inspection Survey Summary ...... 12

LIST OF APPENDICES

APPENDIX A Samples of FBB Calls Recorded During Survey APPENDIX B Photographs

Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

1.0 INTRODUCTION / PROJECT BACKGROUND

Habitat for Humanity of Collier County, Inc. received approval from the South Florida Water Management District (SFWMD) and the US Army Corps of Engineers (USACE) to develop a 100- acre site for a low income residential community known as Kaicasa through Environmental Resource Permit (ERP) No. 11-03097-P and Federal Dredge and Fill Permit SAJ-2005-07439. Due to the economic decline that occurred during the original permit process, the need for this housing has not yet been realized. As such, Habitat for Humanity of Collier County has not started the project and in early 2015 requested an extension of the referenced permits that were due to expire on January 11 and March 17, 2015, respectively.

As part of the review process for the USACE permit extension, the applicant must address the potential for utilization of the property by any new applicable federally listed species that were not considered during original permit review. Since the issuance of the original Dredge and Fill Permit, the Florida bonneted bat (FBB) (Eumops floridanus) was listed as endangered by the US Fish and Wildlife Service (FWS) (effective November 2, 2013) and therefore protected under the Endangered Species Act (ESA). Currently, the FBB has been documented at 17 locations in Charlotte, Collier, Lee, Miami-Dade, Monroe, Okeechobee, and Polk Counties (Marks and Marks, 2012). The Kaicasa project site is located in Section 12, Township 47 South, Range 29 East, in the Immokalee area of Collier County within the FWS Consultation Area for the FBB and approximately 2.8 miles north of the nearest FWS Focal Area for the FBB. The site is bordered by active range land to the north and east, State Road 29 and orange groves to the south, and the Farm Worker Village residential community to the west. A project location map is provided as Figure 1.

1 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

flights can last six hours (NatureServe, 2009) and may occur at distances of several miles from established roosts. The Florida bonneted bat produces loud calls easily recognizable by humans as they fly (Belwood, 1992). Free-tailed (Molossid) bats are known to be high fliers when foraging and acoustic surveys for bonneted bats should take this into consideration when positioning microphones for recording.

Based on previous habitat mapping conducted by Johnson Engineering, Inc., the existing conditions on the project site predominantly include pine flatwoods (30.4 ac.), wet prairie (17.2 ac.), hardwood- conifer mix (15.9 acres), improved upland pasture (10.5 ac.), unimproved pasture (9.7 ac.) and freshwater marsh (9.0 ac.), each with varying degrees of coverage by exotic vegetation, such as melaleuca (Melaleuca quinquenervia) and Brazilian pepper (Schinus terebinthifolius). To ensure that the proposed development would not negatively impact FBB roosting sites, the USACE requested FBB surveys as part of their review for the request for permit extension. This document summarizes the results of both the acoustic surveys and cavity tree/snag inspections that have been conducted on the Kaicasa property between September and November 2015 to determine the use of the property by FBB.

2 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

Figure 1: Location Map

3 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

2.0 METHODOLOGY

2.1 Acoustic Survey

The FBB acoustic surveys were conducted by Johnson Engineering Inc. (JEI) ecologists, with George and Cyndi Marks of the Florida Bat Conservancy (FBC) providing technical support and expert assistance in reviewing and confirming species identification and call types. JEI ecologists have conducted several acoustic bat surveys over the past year using Wildlife Acoustics, SM3BAT ultrasonic bat detectors and also by physically inspecting snags/cavity trees using a wireless cavity inspection camera. The FBC has also provided special training sessions for JEI ecologists on the collection, analysis and interpretation of bat calls with special emphasis on the FBB.

To date, the USFWS has not formalized a standard FBB survey protocol for determining presence/absence, roost identification, or foraging activity. Ecologists at Johnson Engineering, Inc. attended a three day seminar by the FBC to obtain training on equipment and methodologies that can be used to collect and analyze acoustic call data in conducting acoustic surveys for the FBB. The FBC assisted with confirmation of bat species identified by Johnson Engineering via acoustic recording data for the Kaicasa bat surveys.

The Kaicasa acoustic bat surveys were based partially on the methods recommended by the USFWS for the endangered Indiana bat (Myotis sodalis). The objectives of the Indiana bat summer survey guidelines are to (1) standardize range-wide survey procedures; (2) maximize the potential for detection/capture of Indiana bats at a minimum acceptable level of effort; (3) make accurate presence/absence determinations; and (4) aid in conservation efforts for the species by identifying areas where the species is present (USFWS 2014).

In February 2015, Johnson Engineering coordinated with USFWS project manager, Ted Martin and his supervisor, Constance Cassler, for guidance on acceptable bonneted bat acoustic survey protocols for other similar projects to determine if roost surveys would be necessary. The USFWS guidance focused on several factors, including:

4 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

 Detector type:  Detector placement;  Optimal microphone height and orientation based on site specific circumstances;  Suitable weather conditions and nighttime temperatures above 60° Fahrenheit;  Minimum of 5 consecutive nights per survey location;  Use of an equally spaced survey grid pattern with minimum distance of 200 meters (656 feet). USFWS subsequently advised closer spacing (400 – 450 feet apart) to search for roosts; and  Guidelines for data analysis, including calls recorded within 30 minutes of sunrise or sunset.

Based on this coordination with USFWS staff, the Indiana bat survey guidelines were adapted by Johnson Engineering to survey for the FBB at the Kaicasa project site. The entire site (100 ac.) was considered suitable habitat, but with survey focus placed on areas of permitted development impact. Passive acoustic sampling was conducted simultaneously at three (3) survey sites (see Figure 2 for locations) for seven consecutive nights starting before sunset (18:00 hours) and ending Typical SM3 deployment on 3-meter long after sunrise (08:15 hours). Acoustic data metal conduit. U-1 Microphones are situated above the shrub stratum and collection was conducted from September 24, 2015 pointed toward open habitat. through October 2, 2015.

The surveys were conducted using three full spectrum SM3BAT ™ (Wildlife Acoustics; www.wildlifeacoustics.com) bat detectors, each with U1 ultrasonic microphones mounted on a 5- meter long section of metal conduit attached to a tree. The microphone was elevated vertically above the shrub zone and facing open areas to maximize likelihood of recording ultrasonic bat calls. Following data collection, all call sequences were qualitatively identified through visual comparison with a known library of bat calls. Calls were recorded using the full spectrum WAV file format, as recommended by the equipment manufacturer for bat surveys.

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CAVITY TABLE

11 Cavity ID Latitude Longitude 1 26° 24' 16.1" N 81° 23' 1.4" W ASL-5 2 26° 24' 16.1" N 81° 23' 1.6" W 3 26° 24' 16.1" N 81° 23' 1.6" W 3a 26° 24' 16.12" N 81° 23' 1.6" W 4 26° 24' 12.6" N 81° 23' 0.3" W 5 26° 24' 12.0" N 81° 23' 0.3" W d 6 26° 24' 12.6" N 81° 23' 0.5" W x m . 7 26° 24' 9.8" N 81° 22' 55.9" W s n o

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0 FAX (239) 334-3661 DATE PROJECT NO. FILE NO. SCALE SHEET s Collier County, Florida E.B. #642 & L.B. #642 m t f Dec. 2015 20149937-000 -- AS SHOWN 2 \ \ Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

The bottom call frequency range of the FBB is unique to this species and lies between 10-17 kilohertz (kHz). This unique frequency range is a valuable aid in identifying the presence of FBBs.

2.2 Acoustic Data Analysis

Data were recorded on 32 GB SD memory cards, downloaded to a computer and converted to Zero Crossing (ZC) format using Kaleidoscope™ software provided by Wildlife Acoustics. Using the ZC file format, the data was visually reviewed using Analook software (Anabat™Systems). The program settings resulted in recordings of 0.1 to 16 seconds in length and 23,028 recordings were reviewed for detection and subsequent identification of bat species recorded. Review of potential FBB calls was conducted by Cyndi Marks of the FBC. The FBC has been conducting FBB acoustic surveys for over 10 years and is recognized as a technical expert in this field. A summary table was then created to list the number of total calls recorded, total number of confirmed and potential FBB calls, the % FBB, survey begin and end dates, sampling start and end times, number of monitoring days per station, and sunset and sunrise time for the first sampling period at each survey site.

2.3 Roost Survey (Snag/Cavity Tree Inspections) The FBB roost snag/cavity tree inspection was conducted by Johnson Engineering Inc. (JEI) ecologists in accordance with the Draft Protocol for Florida Bonneted Bat Roost Surveys (USFWS, August 2014). The draft protocol requires systematic roost searches in suitable FBB habitat by:  Running line transects through roosting habitat, visually inspecting all trees and snags for evidence of cavities.

 Spacing transects so that all trees and snags are inspected (150’– 300’ apart).

 Transects should be oriented north to south to optimize cavity detectability.

 When cavities are found, their location is recorded in the field using a GPS unit.

 Visually inspect all cavities (except cavities in active RCW trees) using a video probe (peeper) and assess the cavity contents. The camera should be oriented in such a way as to obtain views of both the top and bottom portions of the cavity.

 If bats are suspected or if contents cannot be determined, follow methods to Determine Roost Usage (below) within 2 days of identification/peeping. If the agency or applicant

7 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

does not wish to conduct acoustic roost surveys, an assumption of presence of Florida bonneted bats can be made.

 Record tree species or type of cavity structure, tree diameter and height, cavity height, cavity orientation and cavity contents.

The draft protocol indicates that roosting habitat includes forest and other areas with large or mature trees or other areas with suitable roost structures. Natural roosting structure primarily includes mature or large live or dead trees, tree snags, and trees with cavities, hollows, or crevices. Forest is defined as all types (all age classes) including: pine flatwoods, scrubby flatwoods, pine rocklands, royal palm hammocks, mixed or hardwood hammocks, cypress, sand pine scrub, or other forest types.

Based on previous habitat mapping, the potential roosting habitat on the Kaicasa site includes pine flatwoods (30.4 acres), live oak/cabbage palm (1.6 acres), hardwood-conifer mixed (15.9 acres), and hydric pine flatwoods (1.7 acres).

Two Johnson Engineering, Inc. ecologists established linear transects that were spaced between 150 feet and 250 feet apart based on visibility and the quality of habitat (presence of large mature trees). Large trees and snags were visually inspected on all sides with field binoculars to determine if cavities were present. All trees and snags observed with cavities were GPS marked in the field. Figure 2 shows the survey transects and snags/cavity trees identified during survey.

Once the cavity survey was complete, each tree was visually inspected using an IBWO Wireless Cavity Inspection Camera with Monitor and Digital Video Recorder (ver.2.4X) mounted on a proprietary two-axis radio controlled mount atop a Crain Enterprises, Inc. 35’ tall fiberglass survey rod. The two-axis mount allows the user to obtain views of the top and bottom of portion of the cavities as required by protocol.

Tree species, type of structure, approximate tree diameter and height, approximate cavity height and cavity orientation were recorded for each structure. Finally a Wildlife Acoustics Echo Meter Touch handheld detector, recorder and analyzer was used after each cavity inspection within 15 feet of the tree to determine if audible FBB chirps were present in the area.

8 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

3.0 RESULTS AND DISCUSSION

3.1 Acoustic Survey Results Three acoustic detection devices deployed for seven days each recorded five different bat species. Table 1 provides a summary of the acoustic survey results. Bat species identified during the survey data analysis and their relative abundance based on call frequency within the study area include:  Brazilian free-tailed bat (Tadarida brasiliensis) – Abundant  Evening bat (Nycticeius humeralis) – Common  Northern yellow bat (Lasiurus intermedius) - Uncommon  Big brown bat (Eptesicus fuscus) – Uncommon  Tricolored bat (Perimyotis subflavus) - Uncommon  Florida bonneted bat (Eumops floridanus) – Uncommon (88 individual calls)

The survey recorded 23,028 total bat calls. Of this total, 47 calls recorded at site Two and 42 calls recorded at site Five were confirmed as FBB calls by Johnson Engineering and the FBC. No potential FBB calls were recorded at site Four. The names of the three test sites “Two”, “Four” and “Five” were derived from the SM3 recorder number as designated by JEI. No data was deleted or omitted in this report.

Of greatest interest in searching for a roost site is the time of emergence from the roost shortly after sunset and returning to the same roost before sunrise. Bat calls recorded within 30 minutes of sunset or sunrise may be indicative of a potential roost site in the vicinity. However, FBBs are known to be fast fliers and may have a roost site several miles away even if recorded close to sunset or sunrise (Marks, pers. comm.).

FBB calls recorded at site Two occurred on the evenings of September 24 - 27. The earliest FBB call recorded at site Two was at 21:15:08 on September 25, which was approximately one hour after sunset. The latest FBB call recorded at site Two was at 02:23:32 on September 26, which was nearly five hours before sunrise.

9 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

TABLE 1 – Acoustic Survey Results Summary Table

Site 2 4 5 TOTAL Survey Begin Date 24-Sep-15 24-Sep-15 24-Sep-15 Survey End Date 2-Oct-15 2-Oct-15 2-Oct-15 Survey Nights 7 7 7 Recording Start Time 18:00 18:00 18:00 Sunset 19:15 19:15 19:15 Recording End Time 8:15 8:15 8:15 Sunrise 7:16 7:16 7:16 Total Calls Recorded 7,844 8,478 6,706 23,028 Potential Eumops 50 0 42 92 Confirmed Eumops 47 0 42 89 Percent Eumops 0.60% 0.00% 0.63% 0.39%

FBB calls recorded at site Five occurred on the evenings of September 24 - 28. The earliest FBB call recorded at site Five was at 21:14:20 on September 25, which was approximately one hour after sunset. The latest FBB call recorded at site Five was at 02:17:42 on September 26, which was nearly five hours before sunrise.

If a FBB roost was in close proximity to the meters deployed at Kaicasa, emergence would be expected within 30 minutes before or after sunset. Since the earliest FBB recording occurred approximately one hour after sunset it is unlikely that a roost is located in close proximity to the Kaicasa survey area.

Once the acoustic survey confirmed FBB were flying over or near the site, it was determined the visual inspection of potential roosts would provide clearer evidence of whether or not the Kaicasa project would potentially impact a FBB roost site.

10 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

3.2 Roost Survey (Snag/Cavity Tree Inspection) Results The Kaicasa site includes approximately 49.6 acres of forested habitat. No large areas of dead or dying trees were observed during the survey, and all cavities observed occurred in pine snags. The cavity tree inspection revealed a total of 14 pine snags with cavities or crevices. Closer inspection of the cavity trees revealed 25 cavities or crevices that were large enough to be utilized by FBB for roosting.

For this report, a “cavity” is a round or semi-round hole in the tree typically made by woodpeckers. A “crevice” is a linear crack or split typically caused by the deformation of wood as the tree decays. Table 2 provides a summary of the Cavity Inspection Survey.

No signs of staining or guano were observed on or near any of the 26 cavities or crevices. Additionally, no audible chirping was heard or detected by the Wildlife Acoustics Echo Meter Touch.

Of the 26 cavities and structures found during the survey, 16 were above 35 feet in height, and therefore could not be directly observed. In all cases where the crevice or cavity could be observed, the back of the structure was observed through the peeper camera. The top and bottom of all cavities that were large enough for the peeper camera to fit were visually inspected. No signs of FBB or other wildlife were observed while using the peeper camera.

11 TABLE 2 - CAVITY INSPECTION RESULTS SUMMARY TABLE Structure Structure Staining at Auditory Tree Tree Tree Cavity Cavity Lattitude Longitude Guano Cavity Contents / Comments ID* Type Entrance Chirping Species Diameter height Height Orientation 1 a Snag 26° 24' 16.1" N 81° 23' 1.4" W No No No Pine 16 60 55 South Too high to peep 1 b Snag 26° 24' 16.1" N 81° 23' 1.4" W No No No Pine 16 60 55 South Too high to peep 2 a Snag 26° 24' 16.1" N 81° 23' 1.6" W No No No Pine 20 70 55 South Too high to peep 2 b Snag 26° 24' 16.1" N 81° 23' 1.6" W No No No Pine 20 70 60 South Too high to peep 3 Snag 26° 24' 16.1" N 81° 23' 1.6" W No No No Pine 16 60 58 South Too high to peep 3A a Snag 26° 24' 16.12" N 81° 23' 1.6" W No No No Pine 20 60 40 South Too high to peep 3A b Snag 26° 24' 16.12" N 81° 23' 1.6" W No No No Pine 20 60 42 Southeast Too high to peep 3A c Snag 26° 24' 16.12" N 81° 23' 1.6" W No No No Pine 20 60 45 East Too high to peep 3A d Snag 26° 24' 16.12" N 81° 23' 1.6" W No No No Pine 20 60 50 East Too high to peep 4 a Snag 26° 24' 12.6" N 81° 23' 0.3" W No No No Pine 12 25 15 North Crevice, observed bottom 4 b Snag 26° 24' 12.6" N 81° 23' 0.3" W No No No Pine 12 25 20 North Crevice, observed bottom 5 a Snag 26° 24' 12.0" N 81° 23' 0.3" W No No No Pine 12 25 10 East Crevice, observed bottom 5 b Snag 26° 24' 12.0" N 81° 23' 0.3" W No No No Pine 12 25 20 South Cavity, observed top and bottom 6 a Snag 26° 24' 12.6" N 81° 23' 0.5" W No No No Pine 10 35 17 South Crevice, observed bottom 6 b Snag 26° 24' 12.6" N 81° 23' 0.5" W No No No Pine 10 35 22 Southeast Crevice, observed bottom 6 c Snag 26° 24' 12.6" N 81° 23' 0.5" W No No No Pine 10 35 25 East Cavity, observed top and bottom 6 d Snag 26° 24' 12.6" N 81° 23' 0.5" W No No No Pine 10 35 28 East Cavity, observed top and bottom 7 a Snag 26° 24' 9.8" N 81° 22' 55.9" W No No No Pine 12 55 45 West Too high to peep 7 b Snag 26° 24' 9.8" N 81° 22' 55.9" W No No No Pine 12 55 55 North Too high to peep 8 Snag 26° 24' 7.6" N 81° 23' 3.8" W No No No Pine 12 50 45 South Too high to peep 9 a Snag 26° 24' 4.7" N 81° 23' 3.2" W No No No Pine 10 55 22 Southwest Crevice, observed bottom 9 b Snag 26° 24' 4.7" N 81° 23' 3.2" W No No No Pine 10 55 40 Southwest Too high to peep 10 Snag 26° 23' 52.4" N 81° 23' 2.1" W No No No Pine 14 20 15 Southeast Cavity, observed top and bottom 11 Snag 26° 23' 51.7" N 81° 23' 3.4" W No No No Pine 14 45 45 East Too high to peep 12 Snag 26° 23' 53.9" N 81° 23' 0.5" W No No No Pine 16 55 45 North Too high to peep 13 Snag 26° 24' 11.1" N 81° 22' 54.4" W No No No Pine 12 50 50 West Too high to peep Notes: * Lower case letters represent separate cavities or crevices on the same tree ** Upper case letter "A" next to tree designation represents a separate tree. Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

3.3 Effect Determination

The USFWS has developed a 2013 draft FBB “Effect Determination Key” (Key) to assist regulatory agency reviewers in making effect determinations for projects, such as Kaicasa, located in the FBB consultation area. Based on the project location within the consultation area, project size being greater than 5 acres and presence of more than 1 acre of suitable FBB habitat, the project “may affect” FBBs according to the Key. However, based on the results of the November 2015 acoustic and visual cavity tree surveys, this project is not expected to have a negative impact on the roosting, breeding or foraging of FBBs.

3.4 Conservation Measures to Minimize Impacts

The Kaicasa project is approved for 14.32 acres of stormwater management lakes and includes onsite preserves over 23.63 acres of existing wetlands and 8.52 acres of uplands within the development. Once the project goes to construction, the onsite preserves will be enhanced through exotic removal and managed in perpetuity as required by current permit conditions, which will further enhance onsite foraging and potential roosting opportunities for the FBB. Additionally, Habitat for Humanity has already paid more than 1.5 million dollars for Kaicasa’s offsite mitigation (wetland and listed species mitigation) [3.81 credits from Big Cypress Mitigation Bank (BCMB) and 1,431.37 PHUs from Florida Panther Conservation Bank (FPCB)]. As outlined in the approved BO (Service Federal Activity Code: 41420-2007-FA-0592 / Service Consultation Code: 41420-2007-F-0491) the mitigation that has been purchased to date equates to 182.9 acres of panther habitat. This habitat also falls within the FBB consultation area and provides suitable for FBB. Therefore the mitigation purchased to date should more than offset any potential loss of FBB foraging that may result from the project. As an added conservation measure to further support the overall recovery of the species, the development is also willing to install bat houses within onsite preserves once the project goes to construction.

13 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

4.0 CONCLUSIONS

The acoustic survey revealed 89 confirmed FBB calls by Johnson Engineering and the Florida Bat Conservancy, which equates to approximately 0.39 percent of the 23,028 calls recorded. The earliest FBB calls recorded were at 21:15:08 from Site Two and 21:14:20 from Site Five, which occurred approximately two hours after sunset. The cavity survey revealed no signs of FBB roosting on the Kaicasa site.

Although the USFWS Key tabulates that the Kaicasa project “may affect” FBBs, results from acoustic and cavity inspection surveys suggest that this project is not expected to pose any negative effects on the roosting, breeding, or foraging of FBBs. The proposed onsite lakes, preserves and development areas of Kaicasa will continue providing foraging and potential FBB roosting habitat in the future. Taking this into consideration along with the mitigation that has been purchased to date, a request is made of the USFWS to consider a project determination of “May Affect, Not Likely to Adversely Affect” for the FBB.

14 Florida Bonneted Bat Acoustic and Cavity Inspection Survey Conducted September - November 2015 Habitat for Humanity of Collier County’s “Kaicasa”

5.0 REFERENCES

Belwood, J.J. 1981. Wagner’s mastiff bat, Eumops glaucinus floridanus (Molossidae) in southwestern Florida. Journal of Mammalogy 62:411-413.

Belwood, J.J. 1992. Florida mastiff bat Eumops glaucinus floridanus. Pages 216-223 in S.R. Humphrey (ed.), Rare and endangered biota of Florida. Vol. I. Mammals. University Press of Florida. Gainesville, Florida.

Marks, G.E. and C.S. Marks. 2008. Status of the Florida bonneted bat (Eumops floridanus). Final report. Submitted by the Florida Bat Conservancy for the US Fish and Wildlife Service under grant agreement number 401815G192. Florida Bat Conservancy. Bay Pines, Florida.

Marks, G.E. and C.S. Marks. 2012. Status of the Florida bonneted bat (Eumops floridanus). Final report to the US Fish and Wildlife Service by the Florida Bat Conservancy under grant agreement number 40181AG121. Florida Bat Conservancy. Bay Pines, Florida.

McDonough, M.M., L.K. Ammerman, R.M. Timm, H.H. Genoways, P.A. Larsen, and R.J. Baker. 2008. Speciation within bonneted bats (Genus Eumops): the complexity of morphological, mitochondrial, and nuclear data sets in systematics. Journal of Mammalogy. 89:1306-1315.

Morse, J. 2008. Bat species inventory in the Southwest Region. Florida Fish and Wildlife Conservation Commission, Southwest Region. Lakeland, Florida.

NatureServe. 2009. NatureServe Explorer: An online encyclopedia of life [web application]. Version 7.1. NatureServe, Arlington, Virginia. Available http://explorer.natureserve.org. (Accessed: March 31, 2014).

Robson, M. 1989. Status survey of the Florida mastiff bat. Final Performance Report, Florida Game and Fresh Water Fish Commission, Tallahassee.

Simmons, J.A., M.B. Fenton, and M.J. O’Farrell. 1979. Echolocation and pursuit of prey by bats. Science 203(4375):16-21. 5.

Timm, R.M., and H.H. Genoways. 2004. The Florida bonneted bat, Eumops floridanus (Chiroptera: Molossidae): distribution, morphometrics, systematics, and ecology. Journal of Mammalogy 85:852-86.

APPENDIX A Samples of FBB Calls Recorded During Survey

Typical FBB call recorded at 10:30:40 pm on September 24, 2015 at Site Two.

FBB feeding call recorded at 3:42:35 am on September 28, 2015 at Site Two

FBB below Brazilian free tailed bat recorded at 11:44:12 on September 26, 2015 at Site Two.

FBB below unknown bat species recorded at 12:26:21 am on September 28, 2015 at Site Two.

Insects recorded at 7:06:20 on September 30, 2015 at Site Two.

FBB below Brazilian free tailed bat recorded at 10:54:18 pm on September 24, 2015 at Site Five.

FBB below northern yellow bat recorded at 9:51:27 pm on September 27, 2015 at Site Five.

Evening bat recorded at 8:34:29 on September 24, 2015 at Site Four.

Brazilian free tailed bat recorded at 10:19:36 on September 24, 2015 at Site Four.

Brazilian free tailed bat recorded at 3:08:10 am on September 25, 2015 at Site Five.

Brazilian free tailed bat recorded at 12:53:33 am on October 1, 2015.

Tricolored bat recorded at 9:08:41 on September 28, 2015 at Site Five.

APPENDIX B PHOTOGRAPHS

. Photo of pine snags Photo of wireless during peeper peeper camera at camera survey (CT CT6 during cavity

1-4). Peeper inspection survey. camera is in vertical position to inspect shallow cavities.

Wireless peeper

Wireless peeper camera use at CT 11. camera use at CT 9. Camera is in Camera is in vertical horizontal position position to inspect to inspect deeper shallow cavities. cavities.

Florida Bonneted Bat Acoustic Survey Kaicasa

July 25, 2017

Prepared for:

Habitat for Humanity of Collier County 11145 Tamiami Trail East Naples, FL 34113

Prepared by:

2350 Stanford Court Naples, Florida 34112 (239) 434-0333

L:\20140000\20149937-000 - Habitat for Humanity of Collier Co. (Kaicasa)\FBB\Kaicasa_FBB Acoustic Survey_081017.docx

TABLE OF CONTENTS

Page

1.0 INTRODUCTION / PROJECT BACKGROUND 1.1 Project Information ...... 1 1.2 Florida Bonneted Bat ...... 3 1.3 FBB Survey History ...... 3

2.0 METHODOLOGY 2.1 Acoustic Survey ...... 5 2.2 Acoustic Data Analysis ...... 8 2.3 Acoustic Recording Nights ...... 8

3.0 RESULTS AND DISCUSSION 3.1 Summary of Results ...... 9 3.2 Effect Determination ...... 12 3.3 Conservation Measures to Minimize Impacts ...... 12 3.4 Conclusions ...... 13

4.0 REFFERENCES ...... 15

LIST OF FIGURES AND TABLES

Figure 1 Location Map ...... 2 Figure 2 Acoustic Survey Sites ...... 7

Table 2-1 Ultrasonic Microphone Calibration ...... 8 Table 3-1 Summary of Results from June 2017Acoustic Survey ...... 10 Table 3-2 Summary of Results from Sept 2015 Acoustic Survey ...... 10 Table 3-3 Sepbember 2015 FBB Calls Within Two Hours of Sunset...... 12

LIST OF APPENDICES

APPENDIX A PHOTO DOCUMENTATION APPENDIX B DETAILED WEATHER REPORT DURING ACOUSTIC SURVEY APPENDIC C BAT CALL RECORDINGS

Florida Bonneted Bat Acoustic Survey July 2017 Kaicasa

1.0 INTRODUCTION / PROJECT BACKGROUND

1.1 Project Information:

Habitat for Humanity of Collier County, Inc. received approval from the South Florida Water Management District (SFWMD) and the US Army Corps of Engineers (USACE) to develop a 100-acre site for a low income residential community known as Kaicasa through Environmental Resource Permit (ERP) No. 11-03097-P and Federal Dredge and Fill Permit SAJ-2005-07439. Due to the economic decline that occurred during the original permit process, the need for this housing has not yet been realized. As such, Habitat for Humanity of Collier County has not started the project and in early 2015 requested an extension of the referenced permits that were due to expire on January 11 and March 17, 2015, respectively.

The project is located in Section 12, Township 47 South, Range 29 East, in the Immokalee area of Collier County. The site is bordered by active range land to the north and east, State Road 29 and orange groves to the south, and the Farm Worker Village residential community to the west. A project location map is provided as Figure 1.

1 STATE OF FLORIDA IMMOKALEE RD. / C.R. 846 CHIPLEY ! PENSACOLA ! TALLAHASSEE ! PANAMA CITY LAKE CITY LEGEND ! !

ST. AUGUSTINE 1ST. S. ST NOTES Florida Bonneted ! 1. These drawings are for permitting GAINESVILLE Bat Consultation purposes only and are not intended for ! q Area construction use. 2. Bonneted Bat boundaries shown were Florida Bonneted provided by USFWS. OCALA DAYTONA BEACH PROJECT 3. The aerial photographs shown were !! (!29 Bat Focal Area provided by Collier County government BOUNDARY and were taken in 2016.

PROJECT ORLANDO VICINITY ! SEE STREET MAP AND VICINITY AERIAL TAMPA AT RIGHT ! BARTOW STREET MAP ! N.T.S.

LEE (!82 FORT PIERCE COUNTY (!29 SARASOTA ! xy833 ! IMMOKALEE RD. / C.R. 846 x S T A T ¤£41 HENDRY E R COUNTY WEST PALM BEACH O A FORT MYERS ! D ! 2 9 858 rcgis\bat locmap.mxd §75 xy ¨¦ NAPLES FORT LAUDERDALE !! (!29 MIAMI ! ¨¦§75

PROJECT ¤£41 KEY WEST BOUNDARY Gulf of ! Mexico

VICINITY AERIAL COLLIER COUNTY N.T.S. Sections 12-13 Township 47 South, Range 29 East N.T.S. Latitude: 26° 24' 1" N, Longitude: 81° 22' 59" W \\ftms01\drawings\2014\20149937-000\Environmental\a

JOHNSON ENGINEERING, INC. 2350 STANFORD COURT Location Map Kaicasa NAPLES, FLORIDA 34112 Florida Bonneted Bat Surveys PHONE (239) 434-0333

Collier County, Florida FAX (239) 334-3661 DATE PROJECT NO. FILE NO. SCALE SHEET E.B. #642 & L.B. #642 August 2017 20149937-000 -- N/A Figure 1 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

1.2 Florida Bonneted Bat

The Florida bonneted bat (Eumops floridanus) (FBB) was previously known as the Florida mastiff bat, Wagner’s mastiff bat, and mastiff bat (Eumops glaucinus floridanus). Recent genetic research confirmed that E. floridanus is a distinct species (McDonough, 2008). The FBB is the largest bat in Florida measuring 5.1 to 5.6 inches in length (NatureServe, 2009; Timm and Genoways, 2004). It is distinguished from the Brazilian free-tailed bat (Tadarida brasiliensis) by its larger size and the ears being joined at the midline of the head. (NatureServe, 2009). The Florida bonneted bat’s fur is short and color varies from black to brown to brownish-gray to cinnamon (NatureServe, 2009) (Timm and Genoways, 2004).

The long-term habitat requirements of the Florida bonneted bat are poorly understood (Robson, 1989; Marks and Marks, 2008). Foraging areas for bats are diverse and include fields, ball parks, golf courses, lakes, canals, streams, and wetlands. Perennially flooded wetlands and waterbodies are used as foraging areas during dry seasons and droughts (Marks and Marks, 2008). Bonneted bats rely on speed and agility to catch insects (Simmons, et. al. 1979). An analysis of fecal samples indicates that moths (Lepidoptera), caddisflies (Tricoptera), beetles (Coleoptera), true bugs (Hemiptera), flies (Diptera), and spiders (Arachnida) appear to be important prey items (C. Marks pers. comm.). Echolocation is used to detect prey 10 to 15 feet away (Belwood, 1992). Foraging flights can last six hours (NatureServe, 2009) and may occur at distances of several miles from established roosts. The Florida bonneted bat produces loud calls easily recognizable by humans as they fly (Belwood, 1992). Free-tailed (Molossid) bats are known to be high fliers when foraging and acoustic surveys for bonneted bats should take this into consideration when positioning microphones for recording.

1.3 FBB Survey History

Since the issuance of the original Permit, the FBB was listed as endangered by the US Fish and Wildlife Service (FWS) and threatened by the Florida Fish and Wildlife Conservation Commission (FWC). The Kaicasa project site is located within the FWS Consultation Area for the FBB and approximately 2.8 miles north of the nearest FWS Focal Area for the FBB. Based on previous habitat mapping the existing conditions on the project site predominantly include pine

3 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

flatwoods (30.4 ac.), wet prairie (17.2 ac.), hardwood-conifer mix (15.9 acres), improved upland pasture (10.5 ac.), unimproved pasture (9.7 ac.) and freshwater marsh (9.0 ac.), each with varying degrees of coverage by exotic vegetation, such as melaleuca (Melaleuca quinquenervia) and Brazilian pepper (Schinus terebinthifolius).

To ensure that the proposed development would not negatively impact FBB roosting sites, the USACE required FBB surveys as part of their review for the requested permit extension. A preliminary acoustic survey was conducted in September 2015, in which three Wildlife Acoustics SM3 BAT ultrasonic recorders were deployed for eight nights. Two of the three sites recorded a total of 86 probable FBB calls out of 23,028 total calls equating to approximately 0.37%. With that information, the assumption of presence of FBB was made for the Kaicasa project. In November 2015 a cavity tree survey was performed to identify potential roost sites. All crevices and cavities were inspected for signs of use by FBB including staining, guano, and audible chirps. No signs of use by FBB were detected during the survey. Cavities and crevices were then “peeped” using a wireless camera mounted on a 35-foot tall survey rod with a remote controlled base. The design of the camera allowed the full visual inspection of the top, bottom, and back of all crevices and cavities within 38 feet of the ground. No signs of FBB were observed during the peeping survey. However, since there were cavities and crevices that were higher than 38 feet that were not fully inspected, a followup acoustic survey was requested by FWS. This document provides the results from the second acoustic survey conducted in June 2017, compiled with results from the September 2015 acoustic survey, to determine if FBB utilize the property for roosting.

4 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

2.0 METHODOLOGY

2.1 Acoustic Survey Methodology:

The FBB acoustic surveys were conducted by Johnson Engineering Inc. (JEI) ecologists from the evening of June 9, 2017 through the morning of June 15, 2017 (six nights). JEI ecologists have conducted several acoustic bat surveys over the past year using Wildlife Acoustics, SM3BAT and SM4BAT FS ultrasonic bat detectors and by physically inspecting dead snags using a remote controlled wireless cavity inspection camera allowing the operator to view the back, bottom, sides, and top of crevices and cavities. The Florida Bat Conservancy (FBC) has also provided special training sessions for JEI ecologists on the collection, analysis and interpretation of bat calls with special emphasis on the FBB.

To date, the FWS has not formalized a standard FBB survey protocol for determining presence/absence, roost identification, or foraging activity. Ecologists at Johnson Engineering, Inc. attended a three day seminar by the FBC to obtain training on equipment and methodologies that can be used to collect and analyze acoustic call data in conducting acoustic surveys for the FBB. The FBC assisted with confirmation of bat species identified by Johnson Engineering via acoustic recording data for the Kaicasa bat surveys.

The location and direction of the ultrasonic recorders and microphones was established specifically to determine whether the cavity trees located during previous surveys that were not fully inspected with video equipment contained FBB roosts.

5 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

Through email and phone conversations with Dennis Hamlin, FWS, the following protocols were provided to ensure the cavity trees were properly surveyed with acoustic equipment for the presence of FBB roosts.

A total of seven Acoustic meters would be deployed to collect data from Cavity Trees 1,2,3,7,8,9,11,12 and 13. Protocols for the survey included:

- Verify proper function of detectors and microphones before and after deployment. - Detectors should be positioned 15 meters from potential roost trees. - Microphone should be at least 1.5 meters off the ground oriented in the direction of the potential roost. - Recorder must operate for four consecutive nights from sunset to sunrise. - Appropriate weather conditions consist of: o Nightly temperature above 60 degrees o Any rain than 30 minutes duration o Wind less than 9 mph (4 m/s) Figure 2 provides an aerial photograph with the location of cavity trees that were not fully inspected during the 2015 survey, as well as the deployment location of the ultrasonic microphones and recorders. The follow-up acoustic survey was conducted using five full spectrum SM3BAT and two full spectrum SM4BAT FS (Wildlife Acoustics; www.wildlifeacoustics.com) bat detectors. The SM3BAT detectors utilized U1 ultrasonic microphones, while the SM4BAT FS detectors utilize SMM-U1 microphones. All microphones were mounted on a 5-meter tall metal conduit to elevate the microphone above the shrub level and were attached to a tree or secured in a survey tripod within 15 meters of the cavity tree, and facing toward potential roosts. Following data collection, all call sequences were qualitatively identified through visual comparison with a known library of bat calls. Calls were recorded using the full spectrum WAV file format, as recommended by the equipment manufacturer for bat surveys. The bottom call frequency range of the FBB is unique to this species and lies between 9-17 kilohertz (kHz). This unique frequency range is a valuable aid in identifying the presence of FBBs.

6 CT1, 2 & 3 q 0 250 500

GF SCALE IN FEET

CT13

GF GF CT7

S T A T CT8 E R O A D 2 9 GF CT9

TABLE 1 Map ID Latitude Longitude CT 1 26° 24' 16" N 81° 23' 1" W CT 2 26° 24' 16" N 81° 23' 2" W CT 3 26° 24' 16" N 81° 23' 2" W CT 7 26° 24' 10" N 81° 22' 56" W CT 8 26° 24' 8" N 81° 23' 4" W CT 9 26° 24' 5" N 81° 23' 3" W CT 11 26° 23' 52" N 81° 23' 3" W GF CT 12 26° 23' 54" N 81° 23' 1" W CT 13 26° 24' 11" N 81° 22' 54" W CT12 \\ftms01\drawings\2014\20149937- 000\Environmental\arcgis\[acoustic survey sites table.xlsx]Sheet1 CT11 GF

LEGEND

Project boundary Cavity Trees inspected during the June 2017 Acoustic Survey 15-meter radius (June

rcgis\acoustic survey sites Augustrcgis\acoustic 2017.mxd sites survey 2017 inspected trees) GF Acoustic recorder deployment locations NOTES 1. The aerial photograph shown was Cavity Trees previously provided by Collier County inspected visually government and was taken in 2016.

JOHNSON ENGINEERING, INC. 2350 STANFORD COURT Acoustic Survey Sites NAPLES, FLORIDA 34112 PHONE (239) 434-0333 FAX (239) 334-3661 DATE PROJECT FILE NO. SCALE SHEET E.B. #642 & L.B. #642 August 2017 20149937-000 -- As Shown Figure 2 \\ftms01\drawings\2014\20149937-000\Environmental\a Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

2.2 Acoustic Data Analysis

Data were recorded on 32 GB or 64 GB SD memory cards, downloaded to a computer and converted to Zero Crossing (ZC) format using Kaleidoscope™ software provided by Wildlife Acoustics. Using the ZC file format, the data was visually reviewed using AnaLook software (Anabat™Systems). The program settings resulted in recordings of 0.1 to 16 seconds in length and all recordings were reviewed for detection and subsequent identification of bat species recorded. Results of the survey are detailed in Section 3 below:

Photo documentation of representative detector deployments at Kaicasa is provided in Appendix A. As shown in Table 2-1 below, each microphone was calibrated prior to deployment in accordance with manufacturer guidelines to ensure proper microphone sensitivity.

Table 2-1. Ultrasonic Microphone Calibration

Microphone Standard Project Date Detector ID (dB) Actual Pass / Fail Kaicasa 6/20/2017 7 G -38 -28.16 Pass Kaicasa 6/20/2017 4 C -38 -28.4 Pass Kaicasa 6/20/2017 3 B -38 -31.2 Pass Kaicasa 6/20/2017 2 A -38 -29.7 Pass Kaicasa 6/20/2017 6 F -38 -28.9 Pass Kaicasa 6/20/2017 5 E -38 -28.7 Pass Kaicasa 6/20/2017 1 D -38 -31.3 Pass

2.3 Acoustic Recording Nights

The preliminary acoustic survey conducted in September of 2015 deployed three ultrasonic recorders for eight nights each, totaling 24 recording nights. The follow-up acoustic survey conducted in June of 2017 was intended to record at seven locations for four nights at each location totaling 28 recording nights. Ultrasonic recorders 1 – 6 were deployed for six full nights each. Ultrasonic recorder 7 (CT 8) was deployed for four nights for a total of 40 recording nights.

8 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

3.0 RESULTS AND DISCUSSION 3.1 Summary of Results

As required by the survey protocols, the acceptable weather parameters were nightly temperatures above 60 degrees Farenheit, rain duration of less than 30 minutes, and wind less than 9 mph. Appendix B provides detailed weather reports for the Immokalee Regional Airport located approximately 2.37 miles northwest of the project. Historical weather data are provided by Weather Underground (https://www.wunderground.com/history).

The follow-up acoustic survey recorded 23,857 calls and an additional 4,490 calls classified as noise by the acoustic processing software (total of 28,347 calls). All calls and noise were analysed using Kaliedascope and AnaLook software. As previously stated, the preliminary acoustic survey recorded 23,028 calls and additional 3,135 calls classified as noise. Appendix C provides samples of recordings during the June 2017 survey.

Table 3-1 provides a summary of results for the June, 2017 acoustic survey.

Call sequences from a total of seven bat species were identified during the 40 detector nights of acoustic surveys at the project site. A total of two (2) of the 23,857 calls (0.0084%) were identified as potential FBB calls during the June 2017 survey. Both of these probable FBB calls were recorded from CT 9 at 11:34:25 pm and 11:34:40 pm on June 11. These two calls were recorded immediately after one another and were time-stamped as 20170611 2334 25 and 20170611 2334 40 respectively. This is likely one FBB recording that lasted just over the 16-second recording window.

When all data from the preliminary acoustic survey are combined with the follow-up acoustic survey, there were a total of 46,885 calls recorded along with 7,625 calls classified as noise. A total of 86 probable FBB calls were recorded equating to approximately 0.1877% of the calls. Table 3-2 provides a summary of the preliminary acoustic survey results from September of 2015.

9 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

Table 3-1: Summary of Results from June 2017 Acoustic Survey

Probable Unknown/ Calls Date Date Total FBB potential FBB Meter Site Recorded Noise Deployed Collected Nights Calls Calls 1 CT 13 2,007 504 9-Jun-17 15-Jun-17 6 0 9 2 CT 11 4,888 766 9-Jun-17 15-Jun-17 6 0 2 3 CT 7 2,014 514 9-Jun-17 15-Jun-17 6 0 1 4 CT 9 5,907 800 9-Jun-17 15-Jun-17 6 2 4 5 CT 12 3,163 539 9-Jun-17 15-Jun-17 6 0 1 CT 6 1,2,3 2,634 618 9-Jun-17 15-Jun-17 6 0 3 7 CT 8 3,244 749 9-Jun-17 12-Jun-17 4 0 0

Total 23,857 4,490 40 2 20 Percent Probable FBB Calls 0.0084% Percent Unknown / Potential FBB Calls 0.0838%

Table 3-2: Summary of Results from September 2015 Acoustic Survey

Probable Unknown/ Calls Date Date Total FBB potential FBB Meter Site Recorded Noise Deployed Collected Nights Calls Calls 2 ALS 2 7,844 1,313 24-Sep-15 2-Oct-15 8 44 0 4 ALS 4 8,478 699 24-Sep-15 2-Oct-15 8 0 0 5 ALS 5 6,706 1,123 24-Sep-15 2-Oct-15 8 42 0

Total 23,028 3,135 24 86 0 Percent Probable FBB Calls 0.3735% Percent Unknown / Potential FBB Calls 0.0000%

Additionally, 20 of the 23,857 calls (approximately 0.0838%) recorded during the June 2017 acoustic survey were unknown calls with signatures below 18 kHz. Many of these calls recorded at or above 18 kHz and none of them display the regular, repeating downward sloped charactistics typical of FBB calls.

10 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

Bat species identified during the survey data analysis based on call frequency within the study area include: • Florida bonneted bat (Eumops floridanus) Rare • Brazilian free-tailed bat (Tadarida brasiliensis) Common • Evening bat (Nycticeius humeralis) Common • Northern yellow bat (Lasiurus intermedius) Common • Big brown bat (Eptesicus fuscus) Common • Seminole bat (Lasiurus seminolus) Rare • Tricolored bat (Perimyotis subflavus) Rare

Of greatest interest to FWS in searching for a roost site is the time of emergence from the roost shortly after sunset and returning to the same roost before sunrise. Documentation provided by FWS currently asserts that FBB calls recorded within two hours of emergence may be indicative of potential roosting in the vicinity. However, some experts disagree with this assertion based on the high speeds at which FBBs are known to fly. Research published in November 2016 assessed Brazilian free tailed bat flight behavior and performance and tracked flight speeds up to 99.5 mph, (McCracken 2016). At even half that speed, a FBB could be over 12 miles from its roost within 15 minutes of emergence. Thus, a FBB may have a roost site several miles away even if recorded close to sunset or sunrise (Marks, pers. comm.).

As stated above, a total of two probable FBB calls were recorded at 11:34 pm on June 11, 2017. This recording is three hours, sixteen minutes past sunset (8:18 pm). Additionally, data from the September 2015 acoustic survey was reviewed and revealed that a total of five calls were recorded within two hours (120 minutes) of sunset. Four of those calls came from Site 2 and one from Site 5. Table 3-3 provides the site, call number, date, time, and minutes after sunset for calls recorded within 120 minutes after sunset. The table shows that the call recorded closest to sunset was 20150925_211420 recorded 116 minutes past sunset.

11 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

Table 3-3: September 2015 FBB Calls Within Two Hours of Sunset Minutes Site Call Date Time Sunset After Sunset ALS 2 20150925_211508 9/25/2015 21:15:08 19:18 117 ALS 2 20150925_211518 9/25/2015 21:15:18 19:18 117 ALS 2 20150925_211630 9/25/2015 21:16:30 19:18 118 ALS 2 20150925_211829 9/25/2015 21:18:29 19:18 120 ALS 5 20150925_211420 9/25/2015 21:14:20 19:18 116

If a FBB roost was in close proximity to the survey area, it would be expected that multiple FBB calls would have been detected per night during the survey.

3.2 Effect Determination

The FWS has developed a 2013 draft FBB “Effect Determination Key” (Key) to assist regulatory agency reviewers in making effect determinations for projects located in the FBB consultation area. Based on the project location within the consultation area, project size being greater than 5 acres and presence of more than 1 acre of suitable FBB habitat, the project “may affect” FBBs according to the Key. However, based on the low number of FBB calls detected during the September 2015 and June 2017 acoustic surveys, as well as the negative results of the Roost Survey from November 2015, this project is not expected to adversely affect the roosting, breeding or foraging of FBBs.

3.3 Conservation Measures to Minimize Impacts

The SFWMD ERP (Permit No. 11-03097-P) requires approximately 23.63 acres of enhanced and preserved wetlands and 8.52 acres of uplands retained onsite along with 17.32 acres of stormwater management lakes. Additional offsite mitigation includes 2.89 freshwater herbaceous credits from the Big Cypress Mitigation Bank along with the conservation and

12 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

restoration of 182.94 acres of high quality panther habitat in the Primary Zone (FWS BO, July, 2008)through the purchase of 1,431.37 Panther Habitat Units throuth the Florida Panther Conservation Bank. This combination of habitats provides enhanced forage and roosting opportunities for FBB within onsite preserves and offsite mitigation areas.

FBBs have been documented roosting in trees and man-made structures. Longleaf pines (Pinus palustris), royal palms (Roystonea regia), as well as numerous man-made structures, can provide suitable roosting habitat (Belwood, 1981; Belwood, 1992; Marks and Marks, 2008). Roosts have been documented in Spanish-style tile roofs (Belwood, 1992); in attics, rock or brick chimneys, and fireplaces (NatureServe, 2009); and in bat houses (Marks and Marks, 2008). Therefore, construction of Kaicasa may provide man-made suitable roosting habitat. Existing and proposed surface water management lakes also provide foraging habitat for FBBs should they expand their range to include this project in the future.

3.4 Conclusions

The purpose of this acoustic survey was to ensure no colonies or roosts of FBB were impacted through the construction of this project. It is expected that recording an active FBB roost with calibrated ultrasonic microphones would provide several calls (up to hundreds per recorder-night) with characteristics typical to known FBB recordings. Additionally, recordings of a FBB roost would likely result in many recordings occurring during emergence of the bats near sunset.

The preliminary acoustic survey conducted in September 2015 revealed a total of 23,028 calls with 88 noted as probable FBB calls. Of those, five were recorded within two hours of sunset with the nearest recording occurring 116 minutes past sunset.

The follow-up acoustic survey conducted in June 2017 revealed that two of the 23,857 total call sequences recorded (approximately 0.0084% of the total recorded calls) were classified as probable FBB calls by Johnson Engineering. No probable FBB calls were recorded within two hours of sunset. An additional 20 recordings included data below 18 kHz which did not show characteristic regular, repeated, downward sloping calls, and therefore are not considered as calls in this report.

13 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

The project includes the onsite preservation and enhancement of approximately 23.63 acres of wetlands and 8.52 acres of uplands along with 17.32 acres of stormwater management lakes. Additional offsite mitigation includes 2.89 freshwater herbaceous credits purchased from the Big Cypress Mitigation Bank along with the conservation and restoration of 182.94 acres of high quality panther habitat in the Primary Zone. This combination of habitats provides enhanced forage and roosting opportunities for FBB within onsite preserves and offsite mitigation areas now and into the future.

Although FBB have been recorded at this location, acoustic and cavity tree surveys have shown no evidence of FBB roosts or colonies on the property. This documentation combined with the enhancements to forage and roost habitat associated with wetland and panther mitigation, suggests that this project will have no adverse affect on FBB.

14 Florida Bonneted Bat Acoustic Survey July 22, 2017 Kaicasa

4.0 REFERENCES

Belwood, J.J. 1981. Wagner’s mastiff bat, Eumops glaucinus floridanus (Molossidae) in southwestern Florida. Journal of Mammalogy 62:411-413.

McCracken, Gary F., K. Safi, T.H. Kunz, D.N. Dechmann, S.M. Swartz, M. Wikelski. 2016. Airplane tracking documents the fastest flight speeds recorded for bats. DOI: 10.1098/rsos.160398 (http://rsos.royalsocietypublishing.org/content/3/11/160398). (date accessed 07/25/17)

Morse, J. 2008. Bat species inventory in the Southwest Region. Florida Fish and Wildlife Conservation Commission, Southwest Region. Lakeland, Florida.

Robson, M. 1989. Status survey of the Florida mastiff bat. Final Performance Report, Florida Game and Fresh Water Fish Commission, Tallahassee.

Simmons, J.A., M.B. Fenton, and M.J. O’Farrell. 1979. Echolocation and pursuit of prey by bats. Science 203(4375):16-21. 5.

Timm, R.M., and H.H. Genoways. 2004. The Florida bonneted bat, Eumops floridanus (Chiroptera: Molossidae): distribution, morphometrics, systematics, and ecology. Journal of Mammalogy 85:852-86.

US Department of the Interior, Fish and Wildlife Service, Biological Opinion for the Construction and Operation of the Development Known as Kaicasa. 2008. 54-pgs.

Appendix A Photo Documentation

1 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix A - Photo Documentation July 25, 2017

Typical deployment of SM3 acoustic meter near CT 11. Microphone is approximately 15 feet above ground to reduce interference with surrounding vegetation.

Acoustic recorder microphones were deployed to face the target cavity trees. In this case, a cavity tree cluster including CT 1, 2, and 3. Florida Bonneted Bat Acoustic Survey Kaicasa Appendix A - Photo Documentation July 25, 2017

Typical SM3 acoustic meter deployment.

Deployment of SM3 acoustic recorder at CT 8. Florida Bonneted Bat Acoustic Survey Kaicasa Appendix A - Photo Documentation July 25, 2017

Photograph of CT13. Acoustic meters were mounted to point directly toward cavity trees. Information from the manufacturer indicate that the SM3 and SM4 acoustic meters have an effective range of up to 200 yards.

Photograph of deployment for CT13. Microphones were secured so that they were out of canopy and midstory vegetation to maximize reliability.

Appendix B Detailed Weather Report During Acoustic Survey

2 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 9-Jun-17 7:35 PM 87.8 °F WSW 5.8 mph N/A Mostly Cloudy 9-Jun-17 7:55 PM 84.2 °F East 9.2 mph N/A Overcast 9-Jun-17 8:15 PM 80.6 °F East 5.8 mph N/A Overcast 9-Jun-17 8:35 PM 80.6 °F SE 5.8 mph N/A Scattered Clouds 9-Jun-17 8:55 PM 78.8 °F SE 8.1 mph N/A Partly Cloudy 9-Jun-17 9:15 PM 78.8 °F SE 4.6 mph N/A Clear 9-Jun-17 9:35 PM 77.0 °F ESE 5.8 mph N/A Clear 9-Jun-17 9:55 PM 77.0 °F East 6.9 mph N/A Clear 9-Jun-17 10:15 PM 77.0 °F ESE 6.9 mph N/A Clear 9-Jun-17 10:35 PM 77.0 °F East 5.8 mph N/A Clear 9-Jun-17 10:55 PM 75.2 °F ESE 5.8 mph N/A Scattered Clouds 9-Jun-17 11:15 PM 75.2 °F ESE 4.6 mph N/A Overcast 9-Jun-17 11:35 PM 75.2 °F SE 5.8 mph N/A Overcast 9-Jun-17 11:55 PM 75.2 °F SE 5.8 mph N/A Overcast 10-Jun-17 12:15 AM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 12:35 AM 75.2 °F Calm Calm N/A Partly Cloudy 10-Jun-17 12:55 AM 75.2 °F SE 3.5 mph N/A Clear 10-Jun-17 1:15 AM 75.2 °F Calm Calm N/A Clear 10-Jun-17 1:35 AM 75.2 °F Calm Calm N/A Clear 10-Jun-17 1:55 AM 75.2 °F Calm Calm N/A Clear 10-Jun-17 2:15 AM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 2:35 AM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 2:55 AM 77.0 °F Calm Calm N/A Scattered Clouds 10-Jun-17 3:15 AM 77.0 °F Calm Calm N/A Scattered Clouds 10-Jun-17 3:35 AM 77.0 °F Calm Calm N/A Clear 10-Jun-17 3:55 AM 77.0 °F Calm Calm N/A Clear 10-Jun-17 4:15 AM 75.2 °F Calm Calm N/A Partly Cloudy 10-Jun-17 4:35 AM 75.2 °F East 3.5 mph N/A Clear 10-Jun-17 4:55 AM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 5:15 AM 75.2 °F Calm Calm N/A Overcast 10-Jun-17 5:35 AM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 5:55 AM 75.2 °F Calm Calm N/A Partly Cloudy 10-Jun-17 6:15 AM 75.2 °F NE 3.5 mph N/A Mostly Cloudy 10-Jun-17 6:35 AM 75.2 °F ENE 3.5 mph N/A Overcast 10-Jun-17 6:55 AM 75.2 °F East 4.6 mph N/A Mostly Cloudy 10-Jun-17 7:15 AM 75.2 °F ESE 5.8 mph N/A Mostly Cloudy

1 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 10-Jun-17 7:35 PM 77.0 °F North 5.8 mph N/A Clear 10-Jun-17 7:55 PM 77.0 °F North 4.6 mph N/A Clear 10-Jun-17 8:15 PM 77.0 °F NNE 5.8 mph N/A Clear 10-Jun-17 8:35 PM 77.0 °F Calm Calm N/A Partly Cloudy 10-Jun-17 8:55 PM 75.2 °F NE 3.5 mph N/A Partly Cloudy 10-Jun-17 9:15 PM 75.2 °F East 3.5 mph N/A Partly Cloudy 10-Jun-17 9:35 PM 75.2 °F East 3.5 mph N/A Partly Cloudy 10-Jun-17 9:55 PM 75.2 °F East 4.6 mph N/A Partly Cloudy 10-Jun-17 10:15 PM 75.2 °F Calm Calm N/A Scattered Clouds 10-Jun-17 10:35 PM 75.2 °F Calm Calm N/A Partly Cloudy 10-Jun-17 10:55 PM 75.2 °F East 3.5 mph N/A Clear 10-Jun-17 11:15 PM 75.2 °F Calm Calm N/A Partly Cloudy 10-Jun-17 11:35 PM 75.2 °F Calm Calm N/A Mostly Cloudy 10-Jun-17 11:55 PM 75.2 °F NNE 3.5 mph N/A Overcast 11-Jun-17 12:15 AM 75.2 °F NNE 4.6 mph N/A Mostly Cloudy 11-Jun-17 12:35 AM 75.2 °F Calm Calm N/A Mostly Cloudy 11-Jun-17 12:55 AM 75.2 °F NNE 3.5 mph N/A Mostly Cloudy 11-Jun-17 1:15 AM 75.2 °F NE 4.6 mph N/A Mostly Cloudy 11-Jun-17 1:35 AM 75.2 °F ENE 3.5 mph N/A Mostly Cloudy 11-Jun-17 1:55 AM 75.2 °F NE 4.6 mph N/A Overcast 11-Jun-17 2:15 AM 75.2 °F ENE 4.6 mph N/A Mostly Cloudy 11-Jun-17 2:35 AM 75.2 °F ENE 4.6 mph N/A Mostly Cloudy 11-Jun-17 2:55 AM 75.2 °F ENE 4.6 mph N/A Overcast 11-Jun-17 3:15 AM 75.2 °F Calm Calm N/A Overcast 11-Jun-17 3:35 AM 75.2 °F Calm Calm N/A Overcast 11-Jun-17 3:55 AM 75.2 °F ENE 3.5 mph N/A Mostly Cloudy 11-Jun-17 4:15 AM 75.2 °F East 4.6 mph N/A Mostly Cloudy 11-Jun-17 4:35 AM 77.0 °F East 4.6 mph N/A Overcast 11-Jun-17 4:55 AM 77.0 °F East 3.5 mph N/A Overcast 11-Jun-17 5:15 AM 77.0 °F East 3.5 mph N/A Mostly Cloudy 11-Jun-17 5:35 AM 77.0 °F ENE 3.5 mph N/A Unknown 11-Jun-17 5:55 AM 75.2 °F ENE 4.6 mph N/A Mist 11-Jun-17 6:15 AM 75.2 °F NE 3.5 mph N/A Mist 11-Jun-17 6:35 AM 75.2 °F Calm Calm N/A Mostly Cloudy 11-Jun-17 6:55 AM 75.2 °F ENE 4.6 mph N/A Overcast 11-Jun-17 7:15 AM 77.0 °F NE 4.6 mph N/A Mostly Cloudy

2 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 11-Jun-17 7:35 PM 82.4 °F ENE 8.1 mph N/A Clear 11-Jun-17 7:55 PM 82.4 °F ENE 5.8 mph N/A Clear 11-Jun-17 8:15 PM 80.6 °F ENE 5.8 mph N/A Clear 11-Jun-17 8:35 PM 80.6 °F East 5.8 mph N/A Clear 11-Jun-17 8:55 PM 80.6 °F East 4.6 mph N/A Clear 11-Jun-17 9:15 PM 80.6 °F East 4.6 mph N/A Clear 11-Jun-17 9:35 PM 78.8 °F ENE 4.6 mph N/A Clear 11-Jun-17 9:55 PM 80.6 °F ENE 5.8 mph N/A Clear 11-Jun-17 10:15 PM 78.8 °F East 5.8 mph N/A Clear 11-Jun-17 10:35 PM 78.8 °F East 4.6 mph N/A Clear 11-Jun-17 10:55 PM 78.8 °F East 5.8 mph N/A Clear 11-Jun-17 11:15 PM 77.0 °F Calm Calm N/A Unknown 11-Jun-17 11:35 PM 77.0 °F Calm Calm N/A Unknown 11-Jun-17 11:55 PM 77.0 °F Calm Calm N/A Partly Cloudy 12-Jun-17 12:15 AM 77.0 °F ENE 3.5 mph N/A Mostly Cloudy 12-Jun-17 12:35 AM 77.0 °F NE 3.5 mph N/A Partly Cloudy 12-Jun-17 12:55 AM 77.0 °F ENE 4.6 mph N/A Clear 12-Jun-17 1:15 AM 77.0 °F ENE 4.6 mph N/A Clear 12-Jun-17 1:35 AM 75.2 °F ENE 4.6 mph N/A Unknown 12-Jun-17 1:55 AM 75.2 °F ENE 3.5 mph N/A Unknown 12-Jun-17 2:15 AM 75.2 °F ENE 5.8 mph N/A Mist 12-Jun-17 2:35 AM 77.0 °F ENE 3.5 mph N/A Mist 12-Jun-17 2:55 AM 77.0 °F ENE 4.6 mph N/A Mist 12-Jun-17 3:15 AM 77.0 °F ENE 4.6 mph N/A Mist 12-Jun-17 3:35 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 3:55 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 4:15 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 4:35 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 4:55 AM 77.0 °F Calm Calm N/A Mist 12-Jun-17 5:15 AM 77.0 °F Calm Calm N/A Mist 12-Jun-17 5:35 AM 77.0 °F ENE 4.6 mph N/A Mist 12-Jun-17 5:55 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 6:15 AM 77.0 °F NE 3.5 mph N/A Mist 12-Jun-17 6:35 AM 77.0 °F NE 4.6 mph N/A Mist 12-Jun-17 6:55 AM 77.0 °F Calm Calm N/A Clear 12-Jun-17 7:15 AM 77.0 °F Calm Calm N/A Clear

3 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 12-Jun-17 7:35 PM 78.8 °F NE 4.6 mph N/A Clear 12-Jun-17 7:55 PM 78.8 °F NNE 3.5 mph N/A Clear 12-Jun-17 8:15 PM 77.0 °F Calm Calm N/A Clear 12-Jun-17 8:35 PM 77.0 °F Calm Calm N/A Clear 12-Jun-17 8:55 PM 77.0 °F North 4.6 mph N/A Clear 12-Jun-17 9:15 PM 77.0 °F Calm Calm N/A Clear 12-Jun-17 9:35 PM 77.0 °F NW 3.5 mph N/A Clear 12-Jun-17 9:55 PM 77.0 °F North 4.6 mph N/A Clear 12-Jun-17 10:15 PM 77.0 °F NNE 5.8 mph N/A Clear 12-Jun-17 10:35 PM 77.0 °F NE 4.6 mph N/A Clear 12-Jun-17 10:55 PM 75.2 °F Calm Calm N/A Clear 12-Jun-17 11:15 PM 75.2 °F NNE 3.5 mph N/A Clear 12-Jun-17 11:35 PM 75.2 °F NE 4.6 mph N/A Clear 12-Jun-17 11:55 PM 75.2 °F Calm Calm N/A Partly Cloudy 13-Jun-17 12:15 AM 75.2 °F Calm Calm N/A Clear 13-Jun-17 12:35 AM 75.2 °F NE 4.6 mph N/A Clear 13-Jun-17 12:55 AM 75.2 °F NNE 4.6 mph N/A Clear 13-Jun-17 1:15 AM 75.2 °F NE 3.5 mph N/A Clear 13-Jun-17 1:35 AM 75.2 °F ENE 3.5 mph N/A Clear 13-Jun-17 1:55 AM 75.2 °F ENE 4.6 mph N/A Clear 13-Jun-17 2:15 AM 75.2 °F NE 3.5 mph N/A Clear 13-Jun-17 2:35 AM 75.2 °F NE 4.6 mph N/A Clear 13-Jun-17 2:55 AM 75.2 °F NE 4.6 mph N/A Clear 13-Jun-17 3:15 AM 75.2 °F Calm Calm N/A Clear 13-Jun-17 3:35 AM 73.4 °F Calm Calm N/A Clear 13-Jun-17 3:55 AM 73.4 °F Calm Calm N/A Clear 13-Jun-17 4:15 AM 73.4 °F Calm Calm N/A Clear 13-Jun-17 4:35 AM 73.4 °F ENE 4.6 mph N/A Clear 13-Jun-17 4:55 AM 73.4 °F East 3.5 mph N/A Clear 13-Jun-17 5:15 AM 75.2 °F East 4.6 mph N/A Clear 13-Jun-17 5:35 AM 73.4 °F East 4.6 mph N/A Clear 13-Jun-17 5:55 AM 75.2 °F East 5.8 mph N/A Unknown 13-Jun-17 6:15 AM 75.2 °F East 6.9 mph N/A Clear 13-Jun-17 6:35 AM 75.2 °F Calm Calm N/A Clear 13-Jun-17 6:55 AM 75.2 °F North 3.5 mph N/A Clear 13-Jun-17 7:15 AM 75.2 °F ENE 4.6 mph N/A Clear

4 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 13-Jun-17 7:35 PM 75.2 °F NW 3.5 mph N/A Scattered Clouds 13-Jun-17 7:55 PM 75.2 °F North 4.6 mph N/A Scattered Clouds 13-Jun-17 8:15 PM 75.2 °F Variable 4.6 mph N/A Clear 13-Jun-17 8:35 PM 75.2 °F Calm Calm N/A Clear 13-Jun-17 8:55 PM 73.4 °F NNW 4.6 mph N/A Mostly Cloudy 13-Jun-17 9:15 PM 73.4 °F Calm Calm N/A Partly Cloudy 13-Jun-17 9:35 PM 73.4 °F North 3.5 mph N/A Clear 13-Jun-17 9:55 PM 73.4 °F NNE 4.6 mph N/A Clear 13-Jun-17 10:15 PM 73.4 °F North 4.6 mph N/A Clear 13-Jun-17 10:35 PM 73.4 °F Calm Calm N/A Clear 13-Jun-17 10:55 PM 73.4 °F NNE 4.6 mph N/A Clear 13-Jun-17 11:15 PM 73.4 °F NNE 3.5 mph N/A Clear 13-Jun-17 11:35 PM 73.4 °F NNE 3.5 mph N/A Clear 13-Jun-17 11:55 PM 73.4 °F Calm Calm N/A Clear 14-Jun-17 12:15 AM 73.4 °F NE 3.5 mph N/A Clear 14-Jun-17 12:35 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 12:55 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 1:15 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 1:35 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 1:55 AM 73.4 °F Calm Calm N/A Partly Cloudy 14-Jun-17 2:15 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 2:35 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 2:55 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 3:15 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 3:35 AM 73.4 °F Calm Calm N/A Clear 14-Jun-17 3:55 AM 71.6 °F NW 3.5 mph N/A Partly Cloudy 14-Jun-17 4:15 AM 71.6 °F Calm Calm N/A Partly Cloudy 14-Jun-17 4:35 AM 71.6 °F Calm Calm N/A Clear 14-Jun-17 4:55 AM 71.6 °F Calm Calm N/A Clear 14-Jun-17 5:15 AM 71.6 °F Calm Calm N/A Clear 14-Jun-17 5:35 AM 71.6 °F Calm Calm N/A Clear 14-Jun-17 5:55 AM 71.6 °F Calm Calm N/A Partly Cloudy 14-Jun-17 6:15 AM 71.6 °F Calm Calm N/A Partly Cloudy 14-Jun-17 6:35 AM 71.6 °F Calm Calm N/A Partly Cloudy 14-Jun-17 6:55 AM 71.6 °F NNE 4.6 mph N/A Fog 14-Jun-17 7:15 AM 71.6 °F NNE 3.5 mph N/A Partly Cloudy

5 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix B - Weather Conditions July 25, 2017

Date Time Temp Wind Dir Wind Speed Precip Conditions 14-Jun-17 7:35 PM 82.4 °F East 4.6 mph N/A Clear 14-Jun-17 7:55 PM 82.4 °F East 4.6 mph N/A Clear 14-Jun-17 8:15 PM 80.6 °F Calm Calm N/A Clear 14-Jun-17 8:35 PM 80.6 °F NE 4.6 mph N/A Clear 14-Jun-17 8:55 PM 78.8 °F North - N/A Clear 14-Jun-17 9:15 PM 78.8 °F East 4.6 mph N/A Unknown 14-Jun-17 10:35 PM 77.0 °F East 3.5 mph N/A Clear 14-Jun-17 10:55 PM 78.8 °F ESE 3.5 mph N/A Mostly Cloudy 14-Jun-17 11:15 PM 78.8 °F East 4.6 mph N/A Mostly Cloudy 14-Jun-17 11:35 PM 78.8 °F ESE 5.8 mph N/A Scattered Clouds 14-Jun-17 11:55 PM 78.8 °F East 3.5 mph N/A Mostly Cloudy 15-Jun-17 12:15 AM 77.0 °F SE 5.8 mph N/A Mostly Cloudy 15-Jun-17 12:35 AM 77.0 °F SSE 4.6 mph N/A Mostly Cloudy 15-Jun-17 12:55 AM 77.0 °F SSE 3.5 mph N/A Partly Cloudy 15-Jun-17 1:15 AM 77.0 °F Calm Calm N/A Clear 15-Jun-17 1:35 AM 77.0 °F NE 3.5 mph N/A Clear 15-Jun-17 1:55 AM 77.0 °F Calm Calm N/A Clear 15-Jun-17 2:15 AM 77.0 °F ENE 3.5 mph N/A Clear 15-Jun-17 2:35 AM 75.2 °F East 4.6 mph N/A Clear 15-Jun-17 2:55 AM 77.0 °F Calm Calm N/A Clear 15-Jun-17 3:15 AM 77.0 °F Calm Calm N/A Clear 15-Jun-17 3:35 AM 77.0 °F East 3.5 mph N/A Clear 15-Jun-17 3:55 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 4:15 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 4:35 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 4:55 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 5:15 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 5:35 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 5:55 AM 75.2 °F ESE 4.6 mph N/A Clear 15-Jun-17 6:15 AM 75.2 °F ESE 3.5 mph N/A Clear 15-Jun-17 6:35 AM 75.2 °F Calm Calm N/A Clear 15-Jun-17 6:55 AM 75.2 °F NNE 4.6 mph N/A Clear 15-Jun-17 7:15 AM 77.0 °F Calm Calm N/A Clear

Appendix C Bat Call Recordings

3 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix C - Sample Bat Recordings July 25, 2017

Probable FBB Call at 23:34:25 from CT9, June 11, 2017.

Probable FBB Call recorded at 23:34:40 from CT9, June 11, 2017.

Seminole bat from CT 13, June 9, 2017.

1 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix C - Sample Bat Recordings July 25, 2017

Tricolored bat from CT 13, June 11, 2017.

Brazilian free-tailed bat with unknown recordings at 17 kHz from CT 9, June 13, 2017.

Evening bat from CT 13, June 9, 2017.

2 Florida Bonneted Bat Acoustic Survey Kaicasa Appendix C - Sample Bat Recordings July 25, 2017

Northern yellow bat from CT 13, June 10, 2017.

Big brown bat from CT 13, June 11, 2017.