UT Southwestern George O’Brien Center Mouse models list

The UT Southwestern O’Brien Center strives to facilitate research related to the . To accomplish these goals, we maintain a number of “kidney relevant” mouse lines. All strains are maintained in live colonies and can be rapidly provided upon request through iLabs and completion of required forms. Most lines are transferable within UTSW without an MTA. Transfer outside of UTSW may require an MTA with the originating institution. If you have questions on which mouse line is best for your specific applications or the background strain of your line of interest, please contact Tom Carroll ([email protected], 214-648-4187). Please acknowledge the support of the UTSW Obrien Center (P30DK079328) in all publications.

Available mouse strains Mouse Line Optional information Germline nulls Wnt9b+/- Null allele of Wnt9b Wnt9b neo/+ Partial loss of function allele of Wnt9b Pard3 +/- Vangl2 +/- Fat4+/- Null allele of Fat4 Wnt7b 3-4 deletion Null allele of Wnt7b Wnt7b exon 3 deletion Null allele of Wnt7b Wnt7b exon 1 deletion Partial loss of function allele of Wnt7b Wnt4+/- Null allele of Wnt4 Smoc2-/- Null allele of Smoc2 Fam132a -/- Null allele of Fam132a Fam19a5 -/- Null allele of Fam19a5 (aka Tafa5) Btbd11-/- Null allele of Btbd11 Kl-/- Null allele of Klotho Conditional alleles Wnt9b exon 2 flox Cre inducible null allele of Wnt9b Wnt7b exon3 flox Cre inducible null allele of Wnt7b Wnt7b exon 1 flox Cre inducible partial loss of function allele of Wnt7b Pard3 flox Cre inducible partial loss of function allele of Par3 Lkb1 flox/flox Cre inducible null allele of Lkb1 (Stk11) Pkd1 flox/flox Cre inducible null allele of PKD1

December 30, 2019

Srf flox/flox Cre inducible null allele of Srf Tsc1 flox/flox Cre inducible null allele of Tsc1 Taz flox/flox;Yap Cre inducible null allele of Taz and Yap flox/flox Lats1/2 flox/flox Cre inducible null allele of Lats 1 and 2 Catnb flox/flox Cre inducible null allele of beta-catenin cMyc flox/flox Cre inducible null allele of cMyc Mycn flox/flox Cre inducible null allele of NMyc cMyc/Nmyc flox/flox Cre inducible null allele of cMyc and nMyc Cre activatable cDNAs Catnb exon3 flox Cre activatable allele of beta-catenin Rosa-LSL-DTR Cre activatable allele of Diptheria Toxin receptor Rosa-LSL-DTA Cre activatable allele of Diptheria Toxin A subunit Rosa LSL Yap5SA Cre activatable allele of Yap (5 serines mutated to alanine) Rosa-LSL-Wnt1GFP Cre activatable allele of Wnt1-GFP fusion Cagg-LSL-Wnt9b-IRES- Cre activatable allele of Wnt9b-IRES-GFP GFP Rosa-lsl-rtTA Cre activatable allele of reverse Tet activator (Tet-On) rtTA/tTA inducible cDNAS Tre-MycN Tet-responsive NMyc cDNA Tre-Lin28a Tet-responsive Lin28a cDNA Tre-Lin28b Tet-responsive Lin28b cDNA Tre-DTA Tet-responsive Diphtheria toxin A subunit cDNA Tre-H2B-GFP Tet-responsive 2 B-GFP fusion (nuclear GFP) Dox inducible lines KspCad-tTA Ksp-cadherin driving Tet-activator (Tet-off) cDNA (kidney epithelia driver) KspCad-rtTA Ksp-cadherin promoter driving reverse Tet-activator (Tet-On) cDNA (kidney epithelia driver) Podocin-rtTA Podocin promoter driving reverse Tet-activator (Tet-On) cDNA (podocyte driver) Pdgfrb-rtTA Pdgfrb promoter driving reverse Tet-activator (Tet-On) cDNA (/mural cell driver)

December 30, 2019

Cre lines Pkhd1Cre Cre under control of Pkhd1 promoter (late embryonic/postnatal collecting ducts) Wnt5aCre Cre under control of Wnt5a promoter (posterior mesoderm and renal stroma) Six2Cre-eGFP Cre-GFP fusion under control of Six2 promoter (nephron progenitor cells and extra-renal cell types) Foxd1Cre-eGFP Cre-GFP fusion under control of Foxd1 promoter (renal stromal progenitor cells and extra-renal cell types) Sox2Cre Cre under control of Sox2 promoter (epiblast. Has germline activity in female. Can be used for making null embryos) Rarb2Cre Cre under control of Rarb2 promoter (intermediate mesoderm and extra-renal cell types) Pax3-Cre Cre under control of Pax3 promoter (intermediate mesoderm and extra-renal cell types) Hoxb7Cre Cre under control of Hoxb7 promoter (Wolffian duct and ureteric bud/collecting ducts and extra-renal cell types including posterior neural crest) KspCadherin Cre Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments)

T-CreERT2 Tamoxifen inducible Cre under control of Brachyury promoter (mesoderm) Foxd1CreERT2 Tamoxifen inducible Cre under control of Foxd1 promoter (renal stroma and extra-renal cell types) Osr1CreERT2 Tamoxifen inducible Cre under control of Osr1 promoter (intermediate mesoderm and extra-renal cell types) Six2CreERT2 Tamoxifen inducible Cre under control of Six2 promoter (nephron progenitors and extra-renal cell types) Tcf21 CreERT2 Tamoxifen inducible Cre under control of Tcf21 (pod1) promoter (nephron progenitors, stromal progenitors and extra-renal cell types) CaggCreERT2 Tamoxifen inducible Cre under control of Cagg promoter (ubiquitous in embryo and adult) Smoc2CreERT2 Tamoxifen inducible Cre under control of Smoc2 promoter (outer medullary stroma and broad extra-renal stromal activity)

December 30, 2019

KspCreERT2 Tamoxifen inducible Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments) Slc34a1CreERT2 Tamoxifen inducible Cre under control of Slc34a1 promoter (S1 and S2 segment of proximal tubule) Gli1CreERT2 Tamoxifen inducible Cre under control of Gli1 promoter (subset of embryonic and adult renal stroma and extra renal cell types) Reporter lines Mouse Line Optional information Constitutive reporters Bat-Gal Beta-galactosidase transgene under control of optimized Lef/Tcf binding sites. Reports subset of beta-catenin activity Smoc2-tdTomato tdTomato transgene under control of Smoc2 promoter. Red fluorescence in outer medullary stroma (embryo and adult) and extra renal cell types Mef2c-GFP GFP transgene under control of Mef2c promoter. Green fluorescence in medullary stroma and extra renal cell types Gucy1a3-GFP GFP transgene under control of Gucy1a3 promoter. Green fluorescence in medullary stroma and extra renal cell types Cited1-tomato tdTomato transgene under control of Cited1 promoter. Red fluorescence in nephron progenitor cells and extra renal cell types Pax8-YFP GFP transgene under control of Pax8 promoter. Yellow fluorescence in renal vesicles, Mullerian duct and extra renal cell types Tgfb3-GFP GFP transgene under control of Tgfb promoter. Green fluorescence in cortical stroma, macrophages and extra renal cell types Meis1-GFP GFP transgene under control of Meis1 promoter. Green fluorescence in all renal stroma and extra renal cell types Cre inducible reporters Rosa-LSL- Lacz Beta-galactosidase transgene under control of Rosa26 promoter upon Cre mediated excision of triple polyadenylation site. Rosa-LSL- Myristoylated (membrane) tomato and Myristoylated myrTom/myrGFP (membrane) GFP transgenes under control of Rosa26 promoter. All membranes are red until Cre mediated excision of triple Poly A which allows expression of membrane GFP.

December 30, 2019

Rosa-LSL- Myristoylated (membrane) GFP and Myristoylated myrGFP/myrTom (membrane) Tomato transgenes under control of Rosa26 promoter. All membranes are green until Cre mediated excision of triple Poly A which allows expression of membrane tomato. Rosa-LSL- Tomato Tomato transgene under control of Rosa26 promoter. Cells fluoresce red upon Cre mediated excision of triple polyadenylation site. Rosa-LSL- YFP YFP transgene under control of Rosa26 promoter. Cells fluoresce yellow upon Cre mediated excision of triple polyadenylation site. Rosa-Confetti YFP, CFP and tomato transgenes under control of Rosa26 promoter. Cells stochastically express one of three fluorophores upon Cre mediated excision of triple polyadenylation site. EF1a-LSL-Crb3GFP Crb3-GFP fusion transgene under control of EF1a promoter. Cells express GFP in apical side of membrane upon Cre mediated excision of triple polyadenylation site. Complete Crb3 cDNA is also expressed. Expression may have gain of function phenotypes. Doxycycline inducible reporters Tre-H2bGFP cDNA encoding Histone 2b GFP fusion under control of tet-responsive element. Nuclear GFP can be activated (or inactivated) when combined with tet- repressor or activator lines and doxycycline administration.

December 30, 2019