CUG-Binding 2 (NAPOR)

Catalog number C7948-50D Supplier United States Biological

RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein productsfrom a single structural . Substitutional RNA editing employsa variety of genetic mechanisms, the biochemical basis of whichhas been elucidated following the development of in vitro assaysthat recapitulate important elements of this process. There are two typesof substitutional RNA exist in mammals, namely A-to-I and C-to-URNA editing. The best- characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal (apo B)). Apo B RNA editing changes a CAA to a UAA stop codon,generating a truncated protein, apoB48. The functional complex includes a minimal corecomposed of apobec-1 and ACF, that functionas an adaptor protein by binding both the deaminase and the RNAsubstrate. The RNA binding also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2). CUGBP2/ NAPOR/ Brunol 3/ ETR-3, a novel RNA-binding protein (54kD) found as a component of holoenzyme Apolipoprotein B (apo B) beside apobec-1 and ACF. It plays a role in apo B mRNA editing by forming a regulatory complex with the three components of the minimal editing enzyme, apobec-1, ACF and apo B RNA. Posttranscriptional C to U RNA editing of apo B creates an in-frame stop codon in the edited transcript that in turn results in translation of a truncated protein apo B48. Apo B mRNA editing takes place in mammalian small intestine, it is accordingly an important physiological role in mammalian lipoprotein metabolism. The CUGBP2/ NAPOR is a 509aa long protein (Chr 10p13-p14) exists in three isoforms NAPOR 1, 2 and 3. NAPOR 1 and 2 are same except additional 19aa residues upstream of NAPOR 1, whereas NAPOR 3 differs from other two because of its 5 extra amino residues upstream of N-terminus of NAPOR 2, also 6aa between its second and third RNA binding domains are absent from NAPOR 3. NAPOR 3 is neuron specific in expression whereas the other two are ubiquitous. Applications Western Blot: 1-10ug/ml using ECL technique. ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.

Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Immunogen 18aa peptide from human CUGBP2. Location: ~C-terminus. Species : Mouse, rat - 100%. Formulation Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Purity Purified by immunoaffinity chromatography. Specificity Recognizes human CUGBP2. Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Hu Storage -20°C Reference Shrikant Anant et al (2001) JBC, Vol. 276, No: 50, 47338-47351; Duanxiang Li et al (2001) Genomics 74, 396-401; Lu X et al (1999) Hum. Mol. Genet. 8 (1), 53-60.

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