SANTA CRUZ BIOTECHNOLOGY, INC. CD42b (SZ2): sc-59052

BACKGROUND APPLICATIONS CD42a is a single-chain membrane glycoprotein that forms a noncovalent CD42b (SZ2) is recommended for detection of CD42b of human origin by complex with CD42b. CD42b, also known as a (GPIb a) is a Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), membrane glycoprotein that is composed of a and b chains. The CD42b immunoprecipitation [1-2 µg per 100-500 µg of total (1 ml of cell b chain is also designated CD42c, and is expressed on and megakary- lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- octes. CD42a and CD42b are also present on platelets and megakaryocytes, 1:500) and flow cytometry (1 µg per 1 x 106 cells). and the complex is a major component of the surface. The complex Suitable for use as control antibody for CD42b siRNA (h): sc-42789, CD42b acts as a receptor for von Willebrand’s factor and as a von Willebrand’s shRNA Plasmid (h): sc-42789-SH and CD42b shRNA (h) Lentiviral Particles: factor-dependent adhesion receptor. sc-42789-V. REFERENCES Molecular Weight of CD42b: 143 kDa. 1. Lopez, J.A., et al. 1988. The a and b chains of human platelet glycopro- Positive Controls: human platelet extract: sc-363773. tein Ib are both transmembrane containing a leucine-rich amino acid sequence. Proc. Natl. Acad. Sci. USA 85: 2135-2139. DATA 2. Roth, G.J. 1992. Platelets and blood vessels: the adhesion event. Immunol.

Today 13: 100-105. 200 K – 200 K – 3. Hickey, M.J. and Roth, G.J. 1993. Characterization of the encoding

human platelet glycoprotein IX. J. Biol. Chem. 268: 3438-3443. CD42b 4. Kelly, M.D., et al. 1994. Complementary DNA cloning of the alternatively CD42b expressed endothelial cell glycoprotein Ib (GPIb ) and localization of b b 90 K – 97 K – the GPIb b gene to 22. J. Clin. Invest. 93: 2417-2424.

5. Yagi, M., et al. 1994. Structural characterization and chromosomal loca- CD42b (SZ2): sc-59052. Western blot analysis of CD42b (SZ2): sc-59052. Western blot analysis of tion of the gene encoding human platelet glycoprotein Ib b. J. Biol. Chem. CD42b expression in human platelet extract. CD42b expression in human platelet extract. 269: 17424-17427. SELECT PRODUCT CITATIONS 6. Lopez, J.A., et al. 1994. Glycoprotein (GP)Ib b is the critical subunit linking GPIb a and GPIX in the GPIb-IX complex. Analysis of partial complexes. 1. Navdaev, A., et al. 2011. The rhodocetin ab subunit targets GPIb and J. Biol. Chem. 269: 23716-23721. inhibits induced platelet activation. Toxicon 57: 1041-1048. 7. Kunishima, S., et al. 1994. Bernard-Soulier syndrome Kagoshima: Ser 444Æstop mutation of glycoprotein (GP)Ib a resulting in circulating 2. Li, J., et al. 2014. Neutrophil Akt2 regulates heterotypic cell-cell interac- truncated GPIb a and surface expression of GPIb b and GPIX. Blood 84: tions during vascular inflammation. J. Clin. Invest. 124: 1483-1496. 3356-3362. 3. Tsujimoto, M., et al. 2015. aB-crystallin reduces ristocetin-induced soluble CD40 ligand release in human platelets: suppression of thromboxane A2 CHROMOSOMAL LOCATION generation. Mol. Med. Rep. 12: 357-362. Genetic : GP1BA (human) mapping to 17p13.2. 4. Szklanna, P.B., et al. 2016. Analysis of the proteins associated with platelet detergent resistant membranes. Proteomics 16: 2345-2350. SOURCE 5. Li, J., et al. 2019. Platelet protein disulfide isomerase promotes glyco- CD42b (SZ2) is a mouse monoclonal antibody raised against platelets of protein Iba-mediated platelet-neutrophil interactions under thromboinflam- human origin. matory conditions. Circulation 139: 1300-1319. PRODUCT 6. Liu, F., et al. 2019. T cell-derived soluble glycoprotein GPIba mediates PGE production in human monocytes activated with the vaccine adjuvant Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide, 2 1 MDP. Sci. Signal. 12: eaat6023. 0.1% gelatin and 1% stabilizer protein. 7. Wang, J.D., et al. 2021. Exosomal HMGB1 promoted cancer malignancy. STORAGE Cancers 13: 877. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. PROTOCOLS See our web site at www.scbt.com for detailed protocols and support RESEARCH USE products. For research use only, not for use in diagnostic procedures.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com