Summary of Mouse ARF family GTPases, GAPs, and GEFs NCBI Protein Protein Present in KO DONE (X) GTPases aka MGI # Gene ID Accession Length (aa) Hom v Hs Expression LECA Low priority (*) Allele Mouse mutant phenotypes 1 Arf1 99431 11840 NP_031502.1 181 100 Ubi X Arf1tm1.1Towa/Arf1tm1.1Towa embryonic lethality between implantation and somite formation, complete penetrance 2 Arf2 99595 11841 NP_031503.1 181 NA Ubi, hi NS Arf2tm1.1(KOMP)Wtsi/Arf2tm1.1(KOMP)Wtsi abnormal coat/hair pigmentation; increased hematocrit 3 Arf3 99432 11842 NP_031504.1 181 100 Ubi, v hi NS + Arf3tm1b(EUCOMM)Hmgu/Arf3tm1b(EUCOMM)Hmgu abnormal retina morphology; immune system phenotype 4 Arf4 99433 11843 NP_031505.1 180 96 Ubi Arf4Gt(CSH658)Byg/Arf4Gt(CSH658)Byg embryonic lethality, complete penetrance; other alleles yielding other phenotypes 5 Arf5 99434 11844 NP_031506.1 180 100 Ubi 6 Arf6 99435 11845 NP_031507.1 175 100 Ubi + X Arf6tm1Yaka/Arf6tm1Yaka liver defects, lethality (incomplete penetrance) 7 Arl1 ARFL1 99436 104303 NP_080135.2 181 99 Ubi + 8 Arl2 ARFL2 1928393 56327 NP_062696.2 184 97 Ubi + 9 Arl3 ARFL3 1929699 56350 NP_062692.1 182 98 Ubi + Arl3Gt(OST263303)Lex/Arl3Gt(OST263303)Lex postnatal lethality, kidney/pancreas defects/retinal degeneration 10 Arl4a ARL4 99437 11861 NP_031513.1 200 99 Ubi; hi testis Arl4atm1Asch/Arl4atm1Asch abnormal spermatogenesis 11 Arl4c ARL7 2445172 320982 NP_796279.2 192 100 Hi NS, eye 12 Arl4d ARL6/ARF4L 1933155 80981 NP_079680.1 201 95 Ubi Arl4dtm1a(EUCOMM)Wtsi/Arl4dtm1a(EUCOMM)Wtsi widespread bone defects 13 Arl5a ARL5 1922673 75423 NP_892039.1 179 99 Ubi Arl5atm1a(EUCOMM)Hmgu 14 Arl5b ARL8 1923119 75869 NP_083742.2 180 100 Ubi + Arl5btm1a(EUCOMM)Hmgu 15 Arl5c ARL12 3028577 217151 NP_997114.1 179 82 Thymus, spleen, low others * 16 Arl6 BBS3/RP55 1927136 56297 NP_001334173.1 186 96 Ubi + Arl6tm2Vcs/Arl6tm2Vcs many: brain, retina, sperm flagella 17 Arl8a GIE2/ARL10B 1915974 68724 NP_081099.1 186 99 Ubi Arl8aGt(OST434546)Lex + 18 Arl8b GIE1/ARL10C 1914416 67166 NP_080287.1 186 100 Ubi many, including perinatal lethality 19 Arl9 1915496 384185 NP_996818.1 133-253? ~85 Testis only * Arl9tm1.1(KOMP)Vlcg/Arl9tm1.1(KOMP)Vlcg abnormal heart 20 Arl10 ARL10A 1930788 56795 NP_064352.2 243 86 Ubi X 21 Arl11 ARLTS1 2444054 219144 NP_796311.2 176 86 Tes, lung, lym, others low * 22 Arl13a 1921698 74448 NP_083223.1 372 58 Testis only * Arl13atm1a(KOMP)Wtsi + 23 Arl13b ARL2L1/JBTS8 1915396 68146 NP_080853.3 427 80 Ubi embryonic lethal 24 Arl14 ARF7 1918869 71619 NP_082119.1 192 75 "ambiguous expression data" 25 Arl15 ARFRP2 2442308 218639 NP_766183.1 204 97 Ubi Arl15tm1a(EUCOMM)Wtsi 26 Arl16 1917567 70317 NP_932112.2 173 86 Ubi + X Arl16tm1(KOMP)Vlcg 27 Arfrp1 ARP/ARL18 1923938 76688 NP_001159463.1 201 97 Ubi + Arfrp1tm1Asch/Arfrp1tm1Asch prenatal lethality 28 Sar1a SAR/SARA 98230 20224 NP_033146.1 198 99 Ubi Sar1atm1e(KOMP)Wtsi + 29 Sar1b 1913647 66397 NP_079811.1 198 99 Ubi Sar1btm1a(EUCOMM)Wtsi 30 Trim23 ARD1/ARFD1/RNF46 1933161 81003 NP_001348467.1 574 98 Ubi Trim23tm1a(EUCOMM)Hmgu
Summary of Mouse ARF family GTPases, GAPs, and GEFs NCBI Protein Protein Present in KO DONE (X) GAPs aka MGI # Gene ID Accession Length (aa) Hom v Hs Expression LECA Low priority (*) Allele Mouse mutant phenotypes 1 Arfgap1 2183559 228998 NP_665703.2 414 84 Ubi + Arfgap1tm2a(EUCOMM)Wtsi 2 Arfgap2 1924288 77038 NP_001159496.1 534 92 Ubi Arfgap2tm1a(EUCOMM)Hmgu + 3 Arfgap3 1913501 66251 NP_001344685.1 525 80 Ubi Arfgap3Gt(AY0675)Wtsi 4 Acap1 2388270 216859 NP_722483.2 740 93 Thymus, spleen, limited * Acap1tm1.1(KOMP)Vlcg/Acap1tm1.1(KOMP)Vlcg decreased circulating glucose level 5 Acap2 1925868 78618 NP_084414.1 770 95 Ubi + Acap2tm1a(EUCOMM)Hmgu 6 Acap3 2153589 140500 NP_997106.1 833 94 Ubi Acap3tm1a(EUCOMM)Hmgu/Acap3tm1a(EUCOMM)Hmgu decreased body length 7 Adap1 2442201 231821 NP_766311.2 374 94 Ubi Adap1tm1a(EUCOMM)Wtsi
8 Adap2 2663075 216991 NP_742145.1 381 85 Ubi Adap2tm1a(EUCOMM)Hmgu 9 Agap1 2653690 347722 NP_835220.1 857 98 Ubi Agap1tm1a(EUCOMM)Wtsi/Agap1tm1a(EUCOMM)Wtsi preweaning lethality, incomplete penetrance; eye defects, others 10 Agap2 PIKE 3580016 216439 NP_001028435.1 1186 93 NS, thymus, limited * Agap2tm1.1Kye/Agap2tm1.1Kye mammary defects, glucose metabolism defects 11 Agap3 2183446 213990 NP_631892.2 910 96 Ubi Agap3tm1a(KOMP)Wtsi 12 Agfg1 HRB, RIP 1333754 15463 NP_034602.1 559 97 Ubi + 13 Agfg2 Hrbl, RABR 2443267 231801 NP_001290195.1 490 82 Ubi 15 Arap1 1916960 69710 NP_001035200.1 1441 89 Ubi Arap1tm1(NCOM)Cmhd/Arap1tm1(NCOM)Cmhd decreased lean body mass 16 Arap2 2684416 212285 NP_848494.2 1703 83 Ubi Arap2tm2b(EUCOMM)Wtsi/Arap2tm2b(EUCOMM)Wtsi increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number 17 Arap3 2147274 106952 NP_631945.2 1538 88 Ubi Arap3tm1.1Sve/Arap3tm1.2Sve embryonic lethality during organogenesis, complete penetrance 18 Asap1 1342335 13196 NP_034156.2 1147 95 Ubi Asap1tm1a(KOMP)Wtsi 19 Asap2 2685438 211914 NP_001128664.1 1000 94 Ubi 20 Asap3 2684986 230837 NP_001008233.1 904 81 Ubi 21 Git1 1927140 216963 NP_001004144.1 770 98 Ubi Git1tm1Bcb/Git1tm1Bcb postnatal lethality, many brain/behavior defects
22 Git2 1347053 26431 NP_062808.3 708 92 Ubi Git2tm1Hsb/Git2tm1Hsb lung, spleen, neutrophil changes 23 Smap1 2138261 98366 NP_001344324.1 467 93 Ubi X Smap1tm1.1Sata/Smap1tm1.1Sata many phenotypes + 24 Smap2 1917030 69780 NP_598477.2 428 95 Ubi X 25 ELMOD1 3583900 270162 NP_808437.2 326 96 NS strong bias X Elmod1rda-2J/Elmod1rda-2J deafness, many others 26 ELMOD2 2445165 244548 NP_001164162.1 293 87 Ubi + X Elmod2tm1b(EUCOMM)Hmgu/Elmod2+ several 27 ELMOD3 2445168 232089 NP_001240621.1 381 79 Ubi X Elmod3tm1(KOMP)Vlcg 28 RP2 1277953 19889 NP_598430.1 347 90 Ubi
Summary of Mouse ARF family GTPases, GAPs, and GEFs NCBI Protein Protein Present in KO DONE (X) aka MGI # Gene ID Length (aa) Hom v Hs Expression LECA Low priority (*) Allele Mouse mutant phenotypes GEFs Accession 1 Arfgef1 BIG1 2442988 211673 NP_001095900.1 1846 97 Ubi Arfgef1Gt(CSH465)Byg/Arfgef1Gt(CSH465)Byg neonatal lethality, many NS defects + 2 Arfgef2 BIG2 2139354 99371 NP_001078964.1 1792 95 Ubi Arfgef2Gt(pMS1)16Wen/Arfgef2Gt(pMS1)16Wen embryonic lethality before implantation, complete penetrance 3 Cyth1 Cytohesin 1 1334257 19157 NP_035310.2 398 98 Ubi Cyth1Gt(obn)Byg/Cyth1Gt(obn)Byg 4 Cyth2 ARNO 1334255 19158 NP_035311.1 400 99 Ubi Cyth2tm1a(EUCOMM)Wtsi + 5 Cyth3 ARNO3_GRP1 1335107 19159 NP_035312.3 399 99 Ubi Cyth3tm1a(KOMP)Wtsi 6 Cyth4 2441702 72318 NP_082471.2 393 92 Ubi Cyth4tm1a(EUCOMM)Wtsi 7 Gbf1 1861607 107338 NP_849261.2 1861 94 Ubi + 8 Iqsec1 BRAG2 1196356 232227 NP_001127856.1 1099 92 Ubi Iqsec1tm1.1Hck/Iqsec1tm1.1Hck 9 Iqsec2 BRAG1 3528396 245666 NP_001108136.1 1479 98 Ubi (+?) Iqsec2tm1e(KOMP)Wtsi 10 Iqsec3 BRAG3 2677208 243621 NP_001028526.1 1195 85 NS and a few other Iqsec3tm3b(KOMP)Wtsi/Iqsec3tm3b(KOMP)Wtsi abnormal bone and body mass 11 Psd EFA6A 1920978 73728 NP_001334383.1 1025 92 Ubi Psdtm1a(EUCOMM)Wtsi 12 Psd2 EFA6C 1921252 74002 NP_082983.3 770 88 NS and a few other Psd2tm1a(KOMP)Wtsi
13 Psd3 EFA6D 1918215 234353 NP_808366.2 1004 80 Ubi 14 Psd4 EFA6B 2674093 215632 NP_001342160.1 1005 69 Ubi Psd4tm1a(EUCOMM)Wtsi 15 Fbxo8 1354696 50753 NP_056606.2 319 96 Ubi Fbxo8tm1b(EUCOMM)Wtsi When more than 1 transcript, the longest is typically used