Dr. Munawar Saleem Ahmad Passport number: TP1158651 Father’s Name: Bashir Ahmad Date of Birth: October 28, 1979 Marital Status: Married NIC No.: 33202-4576865-1 Domicile: Jhang (Punjab, ) Contact (Cell): +44-743-845-5095, +92-322-4000024 Email: Till 3rd Oct [email protected] Permanent [email protected]

Current Position And Contact Address Till 3rd Oct. Research Associate and Postdoc fellow (Working on RNAi to block Na ion chanels), School of Biology, Devonshire Building, Newcastle University, Newcastle Upon Tyne, NE1 7RU, UK

After 3rd Oct. Assistant Professor, Department of Zoology, Hazara University, , KP, Pakistan. Research & Teaching (Life Sciences)

Permanent Address Village and Post office LangShumali, Tehsile and District Jhang, Punjab, Pakistan.

Experience

 Working as postdoc fellow to complete a Joint research projest of HEC Pakistan and British Council at School of Biology, Devonshire Building, Newcastle University, Newcastle Upon Tyne, NE1 7RU, UK till 3rd Oct, 2013in UK.  Teaching PhD, Mphil MSc and BSc in Hazara University, Mansehra, Pakistan, since 18- 05-2012 to todate.  Worked as faculty/staff in University of Tennessee, Knoxville, USA for six months 2010- 2011.  Worked as external examiner in BSc practicals for Punjab University, Lahore. Pakistan, 2010-2011.  Worked as visiting faculty in Department of Zoology, Punjab University, Lahore, Pakistan, 2009-2010.  Taken preparatory classes of MCAT (Medical college admission test) at “Students inn” Lahore, 2005-2009.  Worked as examiner for checking papers of FSc for Board of intermediate and secondary education Faisalabad 2002.  Worked as lecturer in Science College Jhang 2001-2003.

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Research Interests  Proteomics  Genomics  Biological control.  Bacillus thuringiensis and its proteins (cry) study.  Study of protein receptors in plasma membrane.  Comparative analysis of proteins of different insects which can serve as binding proteins.  Cloning and expression of cry genes at wild and truncated level of proteins.  Development of cheap media for growth of microbes.  Conducting bioassays with different microbes and insects.

Teaching Interests Biology, Cell and Molecular Biology, Physiology, Biochemistry, Biotechnology, Genetic Engineering, Developmental Biology, Microbiology, Entomology, Signal Transduction, Anatomy, Zoology, Insect Pest Management.

Academic Acheivements Ph.D. (30-04-2012). M.Sc. Zoology, University of the Punjab (P.U.), Lahore, Punjab, Pakistan, (2003).

Places of work during Ph.D. 1. School of Biological Sciences, University of the Punjab, Lahore 2. Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee, USA. 3. National Institute of Agriculture and Biology NIAB, Faisalabad. 4. Ayub Agriculture Research centre Faisalabad.

Ph.D Supervisors Prof. Dr. A.R. Shakoori, Pakistan and Dr. Juan Luis Jurat-Fuentes, USA.

Ph.D Thesis Title Cloning and Molecular Characterization of Cry1c Gene from Local Isolates of Bacillus Thuringiensis having dual toxicity against lepidopteran and dipteran insects..

Techniques Known Well versed with various areas of bioinformatics. Apart from that basic biotechnological techniques, have been learned in immunology, genetic engeneering, molecular biology, biochemistry, microbiology and structural biology.

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Abstract of PhD thesis

Bacillus thuringiensis (Bt.) is gram positive bacterium which forms endospores and produces parasporal crystals. Seventy two samples (soil, cattle waste, straw and insect and bird cadaver) were collected from different localities of Pakistan for isolation of Bt. Isolates were characterized by gram staining and different biochemical tests. Maximum Bt. (80%) were isolated from soil and were screened for presence of cry1Ca and cry1Cb by polymerase chain reaction (PCR). Bioassays were performed to check toxicity against Spodoptera frugiperda, Spodoptera flitura, house fly and mosquito. Three isolates were found positive for cry1Ca and were renamed as MS-SBS-Bt.1-3 while two isolates were found positive for cry1Cb and were renamed as MS-SBS-Bt.4-5. MS-SBS-Bt.1 was most toxic to Spodoptera frugiperda. Full length protein gene for cry1Ca of MS-SBS-Bt.1 was cloned in pTZ57R/T plasmid vector and then this was used as template to amplify different fragments of cry1Ca protein gene. Active toxin region of cry1Ca protein gene was expressed in pET21a+ with or without 28 N-Terminal amino acids of protein. It was found that the cry1C active toxin without first 28 amino acids is lethal to E. coli cells. Various alternative local media have successfully replaced costly commercially available media. We were able to develop an active formulation by using locally available ingredients like water, sugar, antifoam solution, wheat flour, milk powder and gum Arabica. Toxin-receptor interaction was analyzed by using four different cry proteins (cry1Ac, cry1C, cry2Aa and cry1F) and BBMVs from different insects (S. frugiperda, H. zea, CXC, YDK and H. virescens). Western blotting, ligand blotting and alkaline Phosphatase (ALP) assay was performed to identify binding-proteins/receptors. Multiple bands (30-240KDa) were observed in ligand blots and western blots while isolated bands were seen in ALP assays (68-240KDa). From LC-MS/MS data we concluded that the proteins which are possibly important in the mechanism of action of cry proteins are ALP receptors, APN receptors, ABC transporter, Cadherin-like receptors, glycerol-3- phosphate dehydrogenase, ATP synthase b, epoxide hydrolase, elongation factor-1 alpha, shock protein 90, mitochondrial prohibitin complex protein 2, NADPH cytochrome b5 reductase, V-ATPase subunit A, alpha-amylase, spectrin, clathrin, innexin and some other unknown proteins. We are reporting these proteins for the first time which can be probably the receptors of Cry proteins. Further study is required to confirm their role as receptors. 3

Experties 1.PCR optimization 2.Primer designing 3.NCBI BLAST and clastalW homology tree analysis 4.PCR trobleshooting 5.DNA isolation and quantification by different methods. 7.Agarose gel running and interpretation. 8.Restriction of DNA and its interpretation. 9.Bactarial growth and their biochemical and molecular characterization. 10. Transformation of bacteria. 11. Cloning and expression of genes in E. coli. 12.Genotyping of different strains of bacteria. 13.FPLC protein purification analysis. 14.Media and reagents preparation. 15.Protein isolation from intact tissues and their bioassays. 16. Western blotting. 17. ligand blotting. 18. Rearing insects in lab. 19.Grown cotton, tomato, okra in greenhouse and field. 20. Done bioassays with Armyworms, Housefly, Mosquito and different bollworms of cotton. Scientific Conferences /Workshops attended 1. 19th Pakistan Congress of Zoology. April, 19-21, 1999, NARC (PARC), Islamabad, Pakistan.

2. 20th Pakistan Congress of Zoology. March 28-30, 2000, University of Sindh, Jamshoro, Pakistan.

3. Attended international conference of FAOBMB in 2005 in Aiwan-e-Iqbal.

4. 25th Pakistan Congress of Zoology. March 17-19, 2005. Agriculture University, Tandujam, Pakistan.

5. 26th Pakistan Congress of Zoology. April 4-6, 2006, University of the Punjab, Lahore. Pakistan. 6. International symposium on Nanochemistry of enzymes. September 20-21, 2006. School of Biological sciences, University of the Punjab, Lahore, Pakistan.

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7. 27th Pakistan Congress of Zoology. February 27- 1st March, 2007, Bahauddin Zakriya University, Multan, Pakistan.

8. International symposium on glycoproteins in health and diseases, May 27-29, 2008, School of Biological sciences, University of the Punjab, Lahore, Pakistan.

9. 28th Pakistan Congress of Zoology, 2008 Govt. College University, Faisalabad.

10. 29th Pakistan Congress of Zoology. 25- 27 February 2009 University of Sindh, Jamshoro, Pakistan.

11. Conference attended of Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB), International Conference (Lahore, 2005)

12. Workshop attended of FAOBMB, Proteomics & Genomics, Biochemistry International Conference, Lahore. (20-23 November, 2005)

13. Symposium attended on Nanotechnology at School of Biological Sciences, University of the Punjab, Lahore. (May, 2006)

14. Symposium attended on Biomarkers measurements in complex Matrices at School of Biological Sciences, Lahore. (25-26 April, 2007)

15. Symposium attended in MMG dept. proteomics, genomics and metabolomics in Punjab University. (Lahore, 23-25 October 2007)

16. Workshop attended on proteomics, genomics and metabolomics in Punjab University.(Lahore, 23-25 October 2007)

17. Symposium attended of 18th National Chemistry conference in Punjab University. (Lahore, 25-27 February, 2008)

18. Symposium attended on Glycoprotein in SBS, Punjab University, Lahore from 22-23 May, 2008.

19. Workshop attended on “Separation Science and Omics’’ 06-10 April, 2009 in the School of Biological Sciences, University of the Punjab, Lahore.

20. Participated in immuno-assay workshop in sheikh zaid hospital LHR.

21. Life time Fellow Zoological Society of Pakistan. Scientific Conferences /Workshops Conducted at International/National level 1. Member of organizing Committee 25th Pakistan Congress of Zoology. March 17-19, 2005. Agriculture University, Tandujam, Pakistan.

2. Member of organizing Committee 26th Pakistan Congress of Zoology. April 4-6, 2006, University of the Punjab, Lahore. Pakistan.

3. Member of organizing Committee International symposium on Nanochemistry of enzymes. September 20-21, 2006. School of Biological sciences, University of the Punjab, Lahore, Pakistan.

4. Member of organizing Committee 27th Pakistan Congress of Zoology. February 27- 1st March, 2007, Bahauddin Zakriya University, Multan, Pakistan.

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5. Member of organizing Committee International symposium on glycoproteins in health and diseases, May 27-29, 2008, School of Biological sciences, University of the Punjab, Lahore, Pakistan.

6. Member of organizing Committee 28th Pakistan Congress of Zoology, 2008 Govt. College University, Faisalabad.

7. Member of organizing Committee 29th Pakistan Congress of Zoology. 25- 27 February 2009 University of Sindh, Jamshoro, Pakistan.

8. Member of organizing Committee 30th Pakistan Congress of Zoology. February-march 2010, University of agriculture, Faisalabad., Pakistan. Papers Published and Presented 1. Isolation and Molecular Characterization of Cry1C Gene Harboring Bacillus thuringiensis From Different Habitats and Localities of Pakistan. Pakistan Journal of Zoology, 45(1), pp. 261-271, 2013.(http://zsp.com.pk/pdf45/261-271%20_35_%20PJZ- 1218-13%20Munawar-PJZ%201.pdf) 2. Cloning and Molecular Characterization of cry1Ca and cry1Cb Genes from locally isolated Bacillus thuringiensis strains. Pakistan Journal of Zoology, Issue 45(3), pp. 759-778, 2013 (http://zsp.com.pk/pdf45/759-767%20_25_%20PJZ-1356- 13%2025-5-13%20Munawar-PJZ%202%20Revised%2008-05-2013.pdf). 3. Cloning of Cry1C Protein gene of HD137, page 32, 28th Pakistan Congress of Zoology, 2008 Govt. College University, Faisalabad.

4. Formulation of Biopesticide and Cloning of Cry1C, Protein gene of HD137. page 39, 29th Pakistan Congress of Zoology. 25- 27 February, 2009, University of Sindh, Jamshoro, Pakistan

5. Isolation of Bacillus thuringiensis from marine environment and there characterization, 27th Pakistan Congress of Zoology. February 27- 1st March, 2007, Bahauddin Zakriya University, Multan, Pakistan.

6. Cloning of Different Lengths of Toxic Domains of Cry1C Gene from HD137 Strain of Bacillus thuringiensis 30th Pakistan Congress of Zoology, 2010, University of agriculture, Faisalabad.

7. In pipeline to be published: IDENTIFICATION OF CRY1C AND CRY1FA BINDING PROTEINS IN SPODOPTERA FRUGIPERDA USING PROTEOMICS Ahmad, M.S.1’2, Oppert, C.J. 1, Shakoori, A.R.2, Jurat-Fuentas, J.L. 1 8. In pipeline to be published: IDENTIFICATION OF CRY2Aa AND CRY1FA BINDING PROTEINS IN HELIOTHES VIRESCINS USING PROTEOMICS Ahmad, M.S.1’2, Oppert, C.J. 1, Shakoori, A.R.2, Jurat-Fuentas, J.L. 1

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Achievements and Awards 1. Won indigenous PhD scholarship of Higher Education Commission of Pakistan (HEC) for 5 years in 2004. 2. Won IRSIP PhD scholarship of Higher Education Commission of Pakistan (HEC) for 6 months in USA. 3. Got full awards for presenting papers in zoological conferences in 2007, 2008, 2009 and 2010 from University of the Punjab, Lahore, Pakistan. 4. Won Charlace Wallace postdoc fellowship for three months from British Council for year 2012-2013 in University of New Castle, UK. 5. Won a research project worth Rs 0.5m from Higher Education Commission (HEC) of Pakistan for 2012 (Relating the naturally occurring Bacillus thuringiensis (Bt.) with survey of pests (with special focus on lepidoptern and dipteran insects) of economically important crops (tomato, potato, tobacco, cabbage and tea) of Hazara region.)

M.PHIL STUDENT SUPERVISED & AWARDED

REGISTRATIO S.NO NAME OF NAME OF TITLE N DATE - . STUDENT UNIVERISTY COMPLETIO N DATE Genetic evaluation of Lady Amherest, Hazara University, Registration Chrysolophus amherstia Golden Mansehra, KP Date Pheasant, Chrysolophus pictus and 19-12-2012 1 Lubna Yellow Golden, Chrysolophus pictus on Completion the bases of RAPD markers in Date Pheasantry, KP, Pakistan. 12-06-2013 Hazara University, Registration Mansehra, KP Date Seroprevalence of anaplasmosis in sheep, 19-12-2012 2 Muhammad Kashif Ovis aries (L) by ELISA test in Peshawar, Completion Pakistan. Date 12-06-2013

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M.PHIL STUDENTS IN PROGRESS

S.N NAME OF TITLE NAME OF UNIVERSITY REGISTRATI O. STUDENT ON DATE Hazara University, Synopsis Prevalence and characterization of hcv in Mansehra, KP Approved from 1 Khaista Muneer district upper dir, Pakistan BOS Zoo

12-06-2013 Hazara University, Synopsis Insect fauna of tobacco, maize, okra and Mansehra, KP Approved from 2 GAUHAR RAHMAN brinjal in hazara region, Pakistan BOS Zoo

12-06-2013 Hazara University, Synopsis Isolation and characterization of bacillus Mansehra, KP Approved from 3 NUSRAT BAKHT thuringiensis from soil samples of hazara BOS Zoo region 12-06-2013 Study of gene to transcript ratio of DNR-R Hazara University, Synopsis of banana bunchy top virus in infected Mansehra, KP Approved from 4 Nehafta Bibi banana by using qPCR BOS Zoo 12-06-2013 Protective effects of typha latifolia against Hazara University, Synopsis lead nitrate induced oxidative dysfunction Mansehra, KP Approved from 5 Irfan Ullah in cyprinus carpio BOS Zoo 12-06-2013 Hazara University, Synopsis Insect pests of tomato, potato, tea and rice Ghalib Sayyid Mansehra, KP Approved from 6 in hazara region, pakistan BOS Zoo

12-06-2013 Faunistic studies of scarabaeidae Hazara University, Synopsis (coleoptera: scarabaeoidae) of district Mansehra, KP Approved from 7 Sultan Zaib mansehra, kpk, pakistan BOS Zoo 12-06-2013

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References

Prof. Dr. A.R. Shakoori, Prof. Dr. Naeem Rasheed, Director School of Biological Sciences, Professor School of Biological Sciences, University of the Punjab, Lahore. University of the Punjab, Lahore. Pakistan Ph.# +92-42-99230960., Ph.# +92-42-99231534 Email; [email protected], Email; [email protected], [email protected]

Dr. Juan Luis Jurat-Fuentes, Assistant Professor, Institute of Agriculture, Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee, USA. Email; [email protected]. Ph.# 001-865-974-5931

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