Canadian Journal of Zoology

Worms Make Risky Choices Too: The effect of starvation on foraging in the common , terrestris

Journal: Canadian Journal of Zoology

Manuscript ID cjz-2018-0006.R1

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Complete List of Authors: Sandhu, Pawandeep; University of Toronto - Mississauga, Biology Shura, Oskar; University of Toronto - Mississauga, Biology Murray, Rosalind; University of Toronto - Mississauga, Biology; University of Toronto, Ecology and Evolutionary Biology Guy, Cylita; University of Toronto, Ecology and Evolutionary Biology; University of Toronto - Mississauga, Biology

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common earthworm, Lumbricus terrestris, risk-taking, Keyword: FORAGINGDraft< Discipline, life-history trade-offs, starvation

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1 Make Risky Choices Too: The effect of starvation on foraging in the common

2 earthworm,

3 P. Sandhu1§, O. Shura1§, R.L. Murray1,2, and C. Guy1,2 *

4 § Authors contributed equally

5

6 1Biology Dept., University of Toronto at Mississauga, 3359 Mississauga Road

7 Mississauga, ON L5L 1C6, Canada

8 2Dept. of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks Street,

9 Toronto, ON, M5S 3B2, Canada

10

11 Pawandeep Sandhu1: [email protected]

12 Oskar Shura1: [email protected]

13 Rosalind L. Murray1,2: [email protected]

14

15 *Corresponding author:

16 Cylita Guy

17 Dept. of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks Street, Toronto,

18 ON, M5S 3B2, Canada

19 Tel: 6478869524

20 Fax: 4169785878

21 Email: [email protected]

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22 Worms Make Risky Choices Too: The effect of starvation on foraging in the common

23 earthworm, Lumbricus terrestris

24 Pawandeep Sandhu, Oskar Shura, Rosalind L. Murray, and Cylita Guy

25 ABSTRACT

26 Species should avoid risks to protect accumulated fitness. However, when faced with

27 starvation, organisms may accept risks to enhance future reproductive opportunities. We

28 investigated the effect of starvation on risktaking behaviour in the common earthworm,

29 Lumbricus terrestris (Linnaeus, 1758). L. terrestris are negatively phototactic that feed

30 on decaying plant matter at the surface. Feeding in high light conditions is a potentially

31 riskier choice, given the threats of visual predators and desiccation. We predicted that starvation

32 in would increase risktakingDraft behaviour and decrease time taken (latency) to make

33 choices. We manipulated the starvation level of L. terrestris individuals (nonstarved, half

34 starved, and full starved) and presented them with a binary foraging choice. Earthworms could

35 choose either a low food/dark condition (low risk) or a high food/light condition (high risk). We

36 found that starved individuals selected the highrisk condition more often than nonstarved

37 individuals. Starved individuals also had a decreased latency to first choice. Risktaking did not

38 scale with level of starvation; there was no difference in foraging choice and latency between the

39 half and full starved individuals. Our results indicate that L. terrestris makes statedependent

40 foraging choices, providing insight into the importance of fundamental life history tradeoffs in

41 this understudied species.

42 KEYWORDS

43 earthworm, Lumbricus terrestris, risktaking, foraging, lifehistory tradeoffs, starvation

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44

45 INTRODUCTION

46 Foraging presents a costbenefit tradeoff between growth and survival (Puvanendran and

47 Brown 2002). Although necessary for energy acquisition, foraging can put organisms at a greater

48 risk of predation (e.g. Kie 1999). Willingness of prey to take risks while foraging can be

49 influenced by a variety of factors including age (Macrı̀ et al. 2002), reproductive status

50 (Bunnefeld et al. 2006), food availability (Godin and Sproul 1988) and refuge location (van Oers

51 et al. 2005). In particular, changes in risk taking can be strongly influenced by hunger level;

52 when faced with starvation, a variety of taxa have been observed to take greater risks to obtain

53 food (e.g.Lima 1998; Lima and Bednekoff 1999). For example, green sea turtles (Chelonia

54 mydas Linnaeus, 1758) in poor body conditionDraft select higherrisk, but more profitable,

55 microhabitats more frequently than turtles in good body condition (Heithaus et al. 2007). Piglets

56 (Sus domesticus Erxleben, 1777), who are subject to accidental crushing by their mothers while

57 feeding, also make risky choices to feed more often and for longer if in poorer body condition

58 (Weary et al. 1996). Several theoretical foraging models (e.g. McNamara and Houston 1986;

59 Brown 1988; Clark 1994) characterize the tradeoff between body condition and risktaking in the

60 context of protecting accumulated reproductive assets (i.e., ‘asset protection principal’ Clark

61 1994). While exposure to predators puts the entire reproductive asset at risk, starvation reduces

62 the value of this asset, compromising future opportunities for fitness increases (Clark 1994).

63 in better body condition have accumulated fitness to protect. However, hungry

64 individuals can be expected to accept predation risks to increase their reproductive value through

65 increased body condition and, ultimately, enhance future fitness (Clark 1994). Riskdriven

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66 flexibility while foraging, especially in relation to body condition, has been demonstrated in a

67 variety of vertebrate taxa including: mammals (e.g. Hughes and Ward 1993; BergerTal et al.

68 2010), fish (e.g. Biro et al. 2005; Thomson et al. 2012), amphibians (e.g. Whitham and Mathis

69 2000), and birds (e.g. Macleod et al. 2005).

70 Numerous invertebrate species exhibit predator avoidance strategies that involve a trade

71 off between foraging and protection ( e.g. Hernandez and Peckarsky 2014; Szokoli et al. 2015;

72 Blubaugh et al. 2017; also reviewed in Scharf 2016), however, there are relatively few examples

73 documenting statedependent changes in foraging behaviour. Behaviours in response to predator

74 stimuli, such as tube retreat in the Serpula vermicularis Linnaeus,1767 (Dill et

75 al. 1997) and increased dispersal in the backswimmer Notonecta undulata Say, 1832 (McCauley

76 and Rowe 2010), have been shown to limitDraft foraging opportunities. Predator avoidance

77 behaviours, and the potential food stress that may result as a consequence, creates a scenario in

78 which these organisms may also be willing to make risky choices to increase foraging success.

79 Schadegg and Herberholz (2017) demonstrated that in response to a visual danger cue, starved

80 juvenile crayfish (Procmbarus clarkii Girad, 1852) make the risky decision to freeze and remain

81 near a food source, rather than fleeing. Additionally, in response to starvation, negatively

82 phototactic arthropods (i.e., those that move away from light stimuli) display a decreased

83 sensitivity to light (e.g. Shields and Wyman 1984; Dromph 2003), while some beetles spend less

84 time engaging in antipredator death feigning behaviour (Acheampong and Mitchell 1997;

85 Miyatake 2001; Hozumi and Miyatake 2005). However, as indicated in a recent review

86 examining the effects of starvation on arthropods (Scharf 2016), there is room to expand on these

87 studies given the rarity of experiments combining hunger level and predation threat. This is

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88 likely true for other invertebrate taxa, considering the existing taxonomic bias towards

89 vertebrates and model organisms in behaviour research (Rosenthal et al. 2017).

90 We explore hungerdependent risktaking behaviour in the common earthworm,

91 Lumbricus terrestris (Linnaeus, 1758). Earthworms are negatively phototactic (Parker and Arkin

92 1901), a behaviour known to be a predator avoidance strategy in other species such as the

93 polyclad Maritigrella crozieri Hyman, 1939 (Johnson and Forward 2003) and the

94 nematode Caenorhabditis elegans Maupas 1900 (Ward et al. 2008). Earthworms are also a

95 primary food source for a diversity of predators including the red fox (Vulpes vulpes Linnaeus,

96 1758 MacDonald 1980), the ( triangulates Dendy, 1895

97 Boag and Yeates 2001), the European (Meles meles Linnaeus, 1758 Roper 1994), and

98 the carnivorous land snail (PowelliphantaDraft augusta Walker, Trewick & Barker, 2008 Boyer et

99 al. 2011). Although many of these predators use a combination of vision and olfaction to locate

100 or dig for L. terrestris at night (Macdonald 1983), when earthworms are most active (Michiels

101 2001), other predators such as birds may forage for earthworms at dawn (Macdonald 1983) or

102 during the day (Macdonald 1983; Onrust et al. 2017). Thus, negative phototaxis may decrease L.

103 terrestris’ risk of predation, especially from diurnal, opportunistic predators. Additionally, L.

104 terrestris belong to a group of earthworms known as the anecic earthworms, which feed on

105 decaying plant material at the soil’s surface, rather than in underground burrows (Scheu 2003).

106 This soil surface foraging behaviour may be risky for earthworms because it exposes them to

107 potential predators. Although we speculate that the negative phototactic behaviour of L. terrestris

108 relates to predator avoidance, it could also be adaptive for avoiding areas where high light causes

109 arid soil conditions, putting individuals at a greater risk of desiccation. Regardless, the tradeoff

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110 that may exist between predator avoidance and soil surface foraging in L. terrestris makes them

111 an ideal species to examine how starvation can influence risktaking behaviour.

112 In addition to assessing starvation driven risktaking behaviour in L. terrestris, we also

113 characterize the time to first choice, or latency. Predation risk may be expected to impact not

114 only the decision to forage, but other aspects of feeding behaviour as well, including food

115 handling time, acceptable size of prey items, and latency to foraging decisions (Cooper 2000). A

116 negative relationship between latency to attack prey and hunger has been observed in bluntnose

117 minnows (Morgan and Colgan 1987). Changes in latency to risktaking behaviours have also

118 been associated with changes in body condition (Martins et al. 2007) and stress hormones

119 (Mathot and Dingemanse 2015). For example, in salmon (Reinhardt and Healey 1999) food

120 stressed individuals are faster (decreasedDraft latency) to engage in risktaking foraging relative to

121 individuals that are not foodstressed.

122 Beyond being a good system to examine hungerdependent risktaking behaviour, L.

123 terrestris, like many earthworms found in northern North American forests, are an invasive

124 species (Bohlen et al. 2004; Addison 2009; Craven et al. 2017). Considering that L. terrestris is

125 one of the most prominent earthworm invaders in (Addison 2009), studying their

126 behavioural ecology has implications for understanding their spread within ecosystems. Non

127 native earthworm species are ecologically destructive, driving changes in plant diversity and

128 community composition, which may have lasting effects on proper ecosystem functioning

129 (Bohlen et al. 2004; Craven et al. 2017). This highlights the need to manage the spread of these

130 invasive species. Future management efforts would benefit from a greater knowledge of basic

131 earthworm behavioural ecology, as understanding behavioural mechanisms can provide insight

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132 into ecological invasions (Holway and Suarez 1999; Sih et al. 2012) as well as potentially

133 enhance models predicting invader spread (McTavish et al. 2013).

134 Considering the apparent ubiquity of statedependent risktaking in the animal kingdom,

135 it is important to examine whether this behaviour applies broadly by testing it in nontraditional

136 taxa. To our knowledge, no study has yet characterized starvation induced risktaking behaviour

137 in earthworms. Here, we performed a binary choice experiment to assess the effect of starvation

138 on risktaking behaviour in L. terrestris. We hypothesized that, as seen in a variety of other

139 organisms and in line with theoretical expectations of fitness asset protection models (e.g., Clark

140 1994), an increased level of starvation would increase the willingness of L. terrestris to take risks

141 to increase foraging success. Further, we predicted that latency to making risky foraging

142 decisions would decrease as starvation levelDraft increased.

143

144 MATERIALS AND METHODS

145 We obtained L. terrestris individuals from a local bait shop; 15 were used in initial pilot

146 studies and 54 were used in experimental trials (totaling 69 individuals). Worms were housed in

147 groups of no more than 20 in plastic cylindrical containers filled with compost and covered with

148 an opaque black cloth to prevent disturbance by external light. We created three experimental

149 food treatments: nonstarved, halfstarved, and fullstarved individuals. Nonstarved worms were

150 kept in highnutrient compost for seven days prior to the experiment. Fullstarved worms were

151 kept in containers filled with lownutrient potting soil for 7 days prior to the experimental trials.

152 The starvation period was chosen based on the methods of Warne et al. (2001). Halfstarved

153 worms were kept in highnutrient compost for four days and then transferred to lownutrient

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154 potting soil for three days prior to experimental trials (roughly half of the time specified by

155 Warne et al. (2001)). The halfstarved treatment was implemented to test for a threshold in the

156 level of food stress necessary to cause a statedependent reaction in L. terrestris individuals

157 (similar to Morgan and Colgan 1987 and Dromph 2003).

158 Trials were conducted in a 25.4cm x 25.4cm x 5.08cm experimental arena divided into

159 two 12.7cm x 25.4cm x 5.08cm sections. One section (half the arena) was layered with 1.9cm of

160 lownutrient potting soil, while the other half was layered with 1.9cm of highnutrient compost

161 (see supplementary material Figure S1). The lownutrient section of the container was covered

162 with a black opaque cloth to decrease external light, at a height of 2.54cm above the soil. A

163 cardboard bridge, spanning the length of the container and covered with a layer of clear tape (3M

164 Scotch Magic Transparent Tape; to preventDraft the worms from consuming the cardboard), was

165 placed in the middle of the container to separate the low and highnutrient sections. A LED lamp

166 was set up 50.8cm above the container, shining directly on the highnutrient side. Light from

167 LED bulbs can be detected by L. terrestris (Ratner and Gardner 1968)), however LED lamps

168 give off less heat relative to incandescent bulbs (Petroski 2002). As such, we used an LED lamp

169 to decrease the potential effects of heat and soil moisture loss, and increase the likelihood that

170 earthworms were responding to the light cue, rather than a combination of light and heat. The

171 lowfood (potting soil), dark side of the container was considered the lowrisk choice, while the

172 highfood (compost), light side was considered the highrisk choice.

173 We performed a pilot trial to test for an effect of mass on risktaking behaviour in L.

174 terrestris. We also used the pilot trials to assess the time necessary for a worm to evaluate both

175 substrates and make a final choice (see supplementary material Table S2). Finally, we examined

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176 if worms had a preference for the high nutrient compost used here as the highfood condition

177 (see supplementary material Table S3). Our pilot data showed that mass did not affect an

178 individual’s choice (see supplementary material Table S1) and was therefore not included in our

179 future trials. We also found that 12 minute trials allowed for ample time to sample and choose

180 one side/substrate (see supplementary material Table S2). Lastly, we confirmed that earthworm’s

181 had a preference for the high nutrient compost (see supplementary material Table S3).

182 We conducted 18 trials in each of our three starvation conditions (nonstarved, half

183 starved, and fullstarved), with each worm being used only once. For each trial, individuals were

184 placed in the center of the bridge and observed for a maximum of 12 minutes.. During the

185 observation period, we observed all animals sampling both substrates (high and low risk),

186 possibly assessing soil quality through useDraft of chemosensory organs. Earthworms are known to

187 have a variety of sensory cells in the integument (Knapp and Mill 1971), some of which are

188 involved in chemoreception (Syed et al. 2017). For each individual, we recorded final risk choice

189 (high or low) and time to make a final choice (latency). Worms were considered to have made a

190 final choice when their entire body was on one side of the experimental arena. We also recorded

191 the location of the worm (low or highrisk section of arena) after the allotted 12 min trial length.

192 Between trials, potting soil and compost were replaced and the taped cardboard bridge was

193 wiped down with a wet towel.

194

195 Statistical Analyses

196 All statistical analyses were conducted in R (R Core Team 2014). We used the ‘car’ (Fox

197 and Weisberg 2011) and ‘lme4’ (Bates et al. 2014) packages to fit statistical models. To test for

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198 an effect of starvation treatment on an individual’s risk choice (high risk = highfood and bright,

199 low risk = low food and dark), we fit a binomial generalized linear mixed model with risk choice

200 (high or low) as the response, starvation treatment (nonstarved, halfstarved, or fullstarved) as

201 the fixed effect and ‘day’ as a random effect. We also tested for an effect of starvation treatment

202 on an individual’s latency to make a choice regardless of the level of risk. We logtransformed

203 the measure of latency (in seconds) to normalize the data and fit a linear model with latency as

204 the response variable, starvation treatment (nonstarved, halfstarved, or fullstarved) as the fixed

205 effect, and ‘day’ as a random effect.

206

207 RESULTS

208 We found that half and full starvedDraft individuals were more likely to choose the high risk

209 environment compared to nonstarved individuals (Figure 1, Table 1). When we tested for an

210 effect of starvation on latency to make a choice (either high risk or low risk) we found that non

211 starved individuals took longer to make a choice compared to the starved individuals (Figure 2,

212 Table 2). There was no difference between the starvation treatments (half vs. full) in their

213 latency to make a choice, but both starvation treatments were faster to choose than the non

214 starved individuals (Figure 2, Table 2).

215

216 DISCUSSION

217 Statedependent changes in risktaking behvaiour have been documented in a number of

218 species. Here, we demonstrate that starvation alters risktaking behaviour in the common

219 earthworm L. terrestris. In line with our predictions, and the expectations of theoretical fitness

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220 models of foraging (e.g. McNamara and Houston 1986; Brown 1988; Clark 1994), starvation

221 resulted in L. terrestris individuals making riskier choices (Figure 1); starved earthworms were

222 more likely to select the high nutrient, bright light condition than nonstarved individuals (Figure

223 2). We also found that starved individuals, as predicted, had a decreased latency to their choice.

224 Interestingly, although starved individuals made risker and faster choices than nonstarved

225 individuals, we find no evidence for a difference in risk taking or latency between our two

226 starvation conditions (half and full starved). Our results provide strong support for the notion that

227 starvation can mediate life history tradeoffs, leading to increased risktaking behaviour in the

228 common earthworm L. terrestris.

229 There has been a vast amount of literature concerning risktaking behaviour in animals,

230 with numerous studies looking at the tradeoffDraft between feeding and predation risk (Lima and Dill

231 1990). The dependence of this tradeoff on body condition has been demonstrated in a variety of

232 vertebrate taxa (e.g. Hughes and Ward 1993; Biro et al. 2005; Mathot et al. 2015) and is in line

233 with theoretical fitness models of foraging and asset protection (e.g. Clark 1994). Studies

234 examining risk driven flexibility in foraging of invertebrates, although less common, also exist.

235 For example, scorpions in poor body condition, despite the higher predation risk from nocturnal

236 predatory birds, are more likely to forage on fullmoon nights compared to high body condition

237 scorpions (Skutelsky 1996). This tradeoff has also been demonstrated in a variety of other

238 invertebrate species including crayfish (Schadegg and Herberholz 2017), spiders (Walker and

239 Rypstra 2003), mollusks (Zhao et al. 2011), and mayfly larvae (Kohler and McPeek 1989). The

240 results of our study are consistent with the larger field: when challenged with starvation, L.

241 terrestris are more likely to take risks to acquire necessary resources.

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242 Here, we assume that the choice to feed in a high light environment is riskier for L.

243 terrestris as it may increase detection by potential predators. We felt that this was a reasonable

244 assumption given that L. terrestris is a negatively phototactic species and that negative

245 phototaxis is a known antipredator strategy in other groups (e.g. Johnson and Forward 2003;

246 Ward et al. 2008). Further, in pilot studies for this experiment we found that wellfed earthworms

247 have a preference for dark conditions when soil quality is the same (see supplementary material

248 Table S4). Our results indicate that, as seen in other photophobic species (e.g. Shields and

249 Wyman 1984; Dromph 2003), earthworms will tolerate light conditions when under food stress.

250 Although we assumed that foraging in the light represented an increased risk of predation, it

251 could also represent an increased risk of desiccation (we do though control for and minimize heat

252 effects through use of an LED lamp). FutureDraft work should look to characterize risk taking in

253 earthworms in response to a direct predatory cue (e.g. bird calls or feces of a known predator, see

254 Walker and Rypstra 2003), and further examine the ecological conditions under which negative

255 phototaxis may provide an adaptive benefit to L. terrestris.

256 Other factors associated with lifehistory tradeoffs could also be important for mediating

257 risktaking behaviour and resource acquisition in L. terrestris. For example, temperature is

258 associated with increased food intake in juvenile earthworms (Otto 1990), while litter type and

259 conspecific population density have been shown to modify habitat selection in L. terrestris

260 (McTavish et al. 2013). Although we tested for an effect of mass on risk taking in our pilot data,

261 it is also possible that size (assessed with a metric other than mass, such as body length) and age

262 may influence earthworm risktaking behaviour. Previous work in fish suggests that smaller

263 individuals are more willing to take risks than their larger counterparts (Reinhardt and Healey

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264 1999; Dowling and Godin 2002). This sizemediated risk taking behaviour has implications for

265 both individual fitness and population composition (Pettersson and Bronmark 1993; Lima 1998),

266 and could be important for understanding L. terrestris ecology. In addition to considering how

267 size influences risktaking, future work could also examine individual variation in behaviour (i.e.

268 personality) in response to stress (e.g. as in Drent et al. 2003; Houslay et al. 2017). Interspecific

269 variation in behavioural responses to stressors, such as food stress, can impact an individual’s

270 fitness and by extension their success in a given environment (Sih et al. 2012). Considering that

271 L. terrestris is a hugely successful invasive species (Addison 2009), studies such as these would

272 expand on what little we know of the behavioural ecology of this species and could contribute to

273 future management.

274 Our study demonstrates that starvationDraft alters risktaking behaviour in the common

275 earthworm L. terrestris. Relative to nonstarved individuals, starved earthworms select risker

276 environments for foraging and have a decreased latency to their foraging decisions. State

277 dependent tradeoffs, made to maximize longterm fitness, have been observed in a variety of

278 organisms (e.g. Hughes and Ward 1993; BergerTal et al. 2010; Thomson et al. 2012; Schadegg

279 and Herberholz 2017). However, to the best of our knowledge we present the first evidence of

280 statedependent tradeoffs in earthworms and one of the few examples in Annelida. The lack of

281 studies concerning the effects of starvation on foraging speaks to the taxonomic bias in

282 animal behaviour research. A recent study by Rosenthall et al. (2017) quantifies the skew of this

283 bias, indicating that the field has largely focused on higher order taxa leaving other groups,

284 which account for proportionally more of the earth’s biodiversity, relatively understudied. While

285 it remains unclear why such a strong bias exists (e.g. tractability of study systems, innate human

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286 bias, easy study/capture), the authors call for a greater consideration of neglected taxa to help

287 understand the scope and pervasiveness of commonly studied behaviours such as mate selection,

288 predator avoidance, and foraging (Rosenthal et al. 2017). By characterizing an oftenassumed

289 common tradeoff in an understudied species, our study helps to fill this gap by expanding our

290 knowledge about the situations in which statemediated tradeoffs between foraging and

291 predation may exist. As outlined above though, there are a variety of ways in which future work

292 extending this study could enhance our understanding of earthworm behavioural ecology. Given

293 the invasive nature of L. terrestris in North America, this could greatly contribute to their future

294 management.

295

296 ACKNOWLEDGEMENTS Draft

297 We would like to thank D. T. Gwynne and K. Williams for advice and mentorship during

298 the development of this study and the University of Toronto Mississauga’s BIO318 class of 2017

299 for helpful feedback. We would also like to thank Susan Dixon for assistance managing lab

300 space and resources.

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502

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504 TABLES

505

506 Table 1. Results from a binomial generalized linear model investigating the effect of starvation

507 treatment on risktaking behaviour in L. terrestris. Half and full starved individuals were more

508 likely to choose the high risk, high food environment compared to fed individuals. p <0.05 was

509 considered significant.

510

Starvation Estimate z p Treatment ± std error Intercept(Full 0.45±0.48 0.94 0.35 Starved) Half Starved 0.50±0.71 0.71 0.48

1.41±0.71 1.97 0.04 NonStarved Draft 511

512

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518

519

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523

524 Table 2. Results from a linear model investigating the effect of starvation on latency to make a

525 decision (either high risk, high food or low risk, low food) in L. terrestris. Full and half starved

526 individuals were faster to make a choice (low latency measure) compared to nonstarved

527 individuals. Latency (in seconds) was logtransformed prior to inclusion in the model. p <0.05

528 was considered significant.

529

Food stress Estimate t p treatment ± std error Intercept (Full Starved) 5.04±0.21 23.85 <0.001

Half Starved 0.37±0.29 1.25 0.22 Draft 0.83±0.35 2.43 0.02 NonStarved 530

531

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532 FIGURE CAPTIONS

533

534

535 Figure 1. The proportion of Lumbricus terrestris individuals that chose the high risk, high food

536 environment (rather than the low risk, low food environment) across three starvation treatments.

537 Nonstarved individuals were significantly less likely than half and full starved individuals to

538 choose the high risk environment (p=0.04). n=18 for each treatment group. Error bars represent

539 0.95 binomial confidence intervals.

540

541 Figure 2. Mean latency for Lumbricus terrestris to make a choice between a high risk, high food

542 or low risk, low food environment acrossDraft three starvation treatments: nonstarved, half starved

543 and full starved. Fed individuals took significantly longer than half and full starved individuals to

544 make a choice (p=0.02). n=18 for each treatment group. Error bars represent standard error.

545

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Draft

Figure 1. The proportion of Lumbricus terrestris individuals that chose the high risk, high food environment (rather than the low risk, low food environment) across three starvation treatments. Non-starved individuals were significantly less likely than half and full starved individuals to choose the high risk environment (p=0.04). n=18 for each treatment group. Error bars represent 0.95 binomial confidence intervals.

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Draft

Figure 2. Mean latency for Lumbricus terrestris to make a choice between a high risk, high food or low risk, low food environment across three starvation treatments: non-starved, half starved and full starved. Fed individuals took significantly longer than half and full starved individuals to make a choice (p=0.02). n=18 for each treatment group. Error bars represent standard error.

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