Identification, Expression, and Endocrine-Disruption of Three
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Ecdysone: Structures and Functions Guy Smagghe Editor
Ecdysone: Structures and Functions Guy Smagghe Editor Ecdysone: Structures and Functions Editor Guy Smagghe Laboratory of Agrozoology Faculty of Bioscience Engineering Ghent University Belgium ISBN 978-1-4020-9111-7 e-ISBN 978-1-4020-9112-4 Library of Congress Control Number: 2008938015 © 2009 Springer Science + Business Media B.V. No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Printed on acid-free paper springer.com Preface The 16th International Ecdysone Workshop took place at Ghent University in Belgium, July 10–14, 2006 and drew some 150 attendees, many of these young students and postdoctoral associates. These young scientists had the opportunity to dis- cuss their work with many senior scientists at meals, breaks and during the several social events, and were encouraged to do so. This book resulting from the meeting is more up-to-date than might be expected since manuscripts were not delivered to the editor until 2007. The workshop itself had 54 oral presentations as well as many posters. This book, and the meeting itself, is comprised of 23 contributed chapters falling into five general categories: Fundamental Aspects of Ecdysteroid Research: The Distribution and Diversity of Ecdysteroids in Animals and Plants; Ecdysteroid Genetic Hierarchies in Insect Growth and Reproduction; Role of Cross Talk and Growth Factors in Ecdysteroid Titers and Signaling; Ecdysteroid Function Through Nuclear and Membrane Receptors; Ecdysteroids in Modern Agriculture, Medicine, Doping and Ecotoxicology. -
A Comparison of the External Morphology and Functions of Labial Tip Sensilla in Semiaquatic Bugs (Hemiptera: Heteroptera: Gerromorpha)
Eur. J. Entomol. 111(2): 275–297, 2014 doi: 10.14411/eje.2014.033 ISSN 1210-5759 (print), 1802-8829 (online) A comparison of the external morphology and functions of labial tip sensilla in semiaquatic bugs (Hemiptera: Heteroptera: Gerromorpha) 1 2 JOLANTA BROŻeK and HERBERT ZeTTeL 1 Department of Zoology, Faculty of Biology and environmental Protection, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland; e-mail: [email protected] 2 Natural History Museum, entomological Department, Burgring 7, 1010 Vienna, Austria; e-mail: [email protected] Key words. Heteroptera, Gerromorpha, labial tip sensilla, pattern, morphology, function, apomorphic characters Abstract. The present study provides new data on the morphology and distribution of the labial tip sensilla of 41 species of 20 gerro- morphan (sub)families (Heteroptera: Gerromorpha) obtained using a scanning electron microscope. There are eleven morphologically distinct types of sensilla on the tip of the labium: four types of basiconic uniporous sensilla, two types of plate sensilla, one type of peg uniporous sensilla, peg-in-pit sensilla, dome-shaped sensilla, placoid multiporous sensilla and elongated placoid multiporous sub- apical sensilla. Based on their external structure, it is likely that these sensilla are thermo-hygrosensitive, chemosensitive and mechano- chemosensitive. There are three different designs of sensilla in the Gerromorpha: the basic design occurs in Mesoveliidae and Hebridae; the intermediate one is typical of Hydrometridae and Hermatobatidae, and the most specialized design in Macroveliidae, Veliidae and Gerridae. No new synapomorphies for Gerromorpha were identified in terms of the labial tip sensilla, multi-peg structures and shape of the labial tip, but eleven new diagnostic characters are recorded for clades currently recognized in this infraorder. -
Forest Health Conditions in Ontario, 2017
Forest Health Conditions in Ontario, 2017 Ministry of Natural Resources and Forestry Forest Health Conditions in Ontario, 2017 Compiled by: • Ontario Ministry of Natural Resources and Forestry, Science and Research Branch © 2018, Queen’s Printer for Ontario Printed in Ontario, Canada Find the Ministry of Natural Resources and Forestry on-line at: <http://www.ontario.ca>. For more information about forest health monitoring in Ontario visit the natural resources website: <http://ontario.ca/page/forest-health-conditions> Some of the information in this document may not be compatible with assistive technologies. If you need any of the information in an alternate format, please contact [email protected]. Cette publication hautement spécialisée Forest Health Conditions in Ontario, 2017 n'est disponible qu'en anglais en vertu du Règlement 671/92 qui en exempte l’application de la Loi sur les services en français. Pour obtenir de l’aide en français, veuillez communiquer avec le ministère des Richesses naturelles au <[email protected]>. ISSN 1913-617X (Online) ISBN 978-1-4868-2275-1 (2018, pdf) Contents Contributors ........................................................................................................................ 4 État de santé des forêts 2017 ............................................................................................. 5 Introduction......................................................................................................................... 6 Contributors Weather patterns ................................................................................................... -
Nuclear Receptor Ftz-F1 Promotes Follicle Maturation and Ovulation
RESEARCH ARTICLE Nuclear receptor Ftz-f1 promotes follicle maturation and ovulation partly via bHLH/PAS transcription factor Sim Elizabeth M Knapp1, Wei Li1, Vijender Singh2, Jianjun Sun1,2* 1Department of Physiology & Neurobiology, University of Connecticut, Storrs, United States; 2Institute for Systems Genomics, University of Connecticut, Storrs, United States Abstract The NR5A-family nuclear receptors are highly conserved and function within the somatic follicle cells of the ovary to regulate folliculogenesis and ovulation in mammals; however, their roles in Drosophila ovaries are largely unknown. Here, we discover that Ftz-f1, one of the NR5A nuclear receptors in Drosophila, is transiently induced in follicle cells in late stages of oogenesis via ecdysteroid signaling. Genetic disruption of Ftz-f1 expression prevents follicle cell differentiation into the final maturation stage, which leads to anovulation. In addition, we demonstrate that the bHLH/PAS transcription factor Single-minded (Sim) acts as a direct target of Ftz-f1 to promote follicle cell differentiation/maturation and that Ftz-f1’s role in regulating Sim expression and follicle cell differentiation can be replaced by its mouse homolog steroidogenic factor 1 (mSF-1). Our work provides new insight into the regulation of follicle maturation in Drosophila and the conserved role of NR5A nuclear receptors in regulating folliculogenesis and ovulation. Introduction *For correspondence: [email protected] Female fertility, an essential half of the reproductive equation, requires proper follicle maturation and ovulation. The NR5A family of nuclear receptors are critical for the success of these complex Competing interests: The ovarian processes across species (Jeyasuria et al., 2004; Meinsohn et al., 2019; Mlynarczuk et al., authors declare that no 2013; Sun and Spradling, 2013; Suresh and Medhamurthy, 2012). -
Characterization of the Novel Role of Ninab Orthologs from Bombyx Mori and Tribolium Castaneum T
Insect Biochemistry and Molecular Biology 109 (2019) 106–115 Contents lists available at ScienceDirect Insect Biochemistry and Molecular Biology journal homepage: www.elsevier.com/locate/ibmb Characterization of the novel role of NinaB orthologs from Bombyx mori and Tribolium castaneum T Chunli Chaia, Xin Xua, Weizhong Sunb, Fang Zhanga, Chuan Yea, Guangshu Dinga, Jiantao Lia, ∗ Guoxuan Zhonga,c, Wei Xiaod, Binbin Liue, Johannes von Lintigf, Cheng Lua, a State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Southwest University, Chongqing 400715, China b College of Animal Science and Technology, Southwest University, Chongqing 400715, China c Life Sciences Institute and the Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China d College of Plant Protection, Southwest University, Chongqing 400715, China e Sericulture Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, China f Department of Pharmacology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA ABSTRACT Carotenoids can be enzymatically converted to apocarotenoids by carotenoid cleavage dioxygenases. Insect genomes encode only one member of this ancestral enzyme family. We cloned and characterized the ninaB genes from the silk worm (Bombyx mori) and the flour beetle (Tribolium castaneum). We expressed BmNinaB and TcNinaB in E. coli and analyzed their biochemical properties. Both enzymes catalyzed a conversion of carotenoids into cis-retinoids. The enzymes catalyzed a combined trans to cis isomerization at the C11, C12 double bond and oxidative cleavage reaction at the C15, C15′ bond of the carotenoid carbon backbone. Analyses of the spatial and temporal expression patterns revealed that ninaB genes were differentially expressed during the beetle and moth life cycles with high expression in reproductive organs. -
Molecular Biology of Bhlh PAS Genes Involved in Dipteran Juvenile Hormone Signaling
Molecular Biology of bHLH PAS Genes Involved in Dipteran Juvenile Hormone Signaling Dissertation Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Aaron A. Baumann. B.S. Graduate Program in Entomology The Ohio State University 2010 Dissertation Committee: Thomas G. Wilson, Advisor David Denlinger H. Lisle Gibbs Amanda Simcox Copyright by Aaron A. Baumann 2010 Abstract Methoprene tolerant (Met), a member of the bHLH-PAS family of transcriptional regulators, has been implicated in juvenile hormone (JH) signaling in Drosophila melanogaster. Met mutants are resistant to the toxic and morphogenetic defects of exogenous JH application. A paralogous gene in D. melanogaster, germ cell expressed (gce), forms JH-sensitive heterodimers with MET, but a function for gce has not been reported. DmMet orthologs from three mosquito species are characterized and, based on sequence analysis and intron position, are shown to have higher sequence identity to Dmgce than to DmMet. An evolutionary scheme for the origin of Met from a gce-like ancestor gene in lower Diptera is proposed. RNAi-driven underexpression of Met in the Yellow Fever mosquito, Aedes aegypti, results in the concomitant reduction of putative JH-inducible genes, suggesting involvement in JH signaling. The viability of D. melanogaster Met mutants is thought to result from functional redundancy conferred by gce. Therefore, genetic manipulation of gce expression was used to probe the function of this gene. Overexpression of gce was shown to alleviate preadult, but not adult Met phenotypes. RNAi-driven underexpression of gce resulted in ii preadult lethality in both Met+ and Met mutant backgrounds. -
STATE of the ART ASSESSMENT of ENDOCRINE DISRUPTERS Final Report
STATE OF THE ART ASSESSMENT OF ENDOCRINE DISRUPTERS Final Report Project Contract Number 070307/2009/550687/SER/D3 Authors: Andreas Kortenkamp, Olwenn Martin, Michael Faust, Richard Evans, Rebecca McKinlay, Frances Orton and Erika Rosivatz 23.12.2011 TABLE OF CONTENTS TABLE OF CONTENTS 0 EXECUTIVE SUMMARY ......................................................................................................................... 7 1 INTRODUCTION .................................................................................................................................... 9 1.1 TERMS OF REFERENCE, SCOPE OF THE REPORT ........................................................................... 9 1.2 STRUCTURE OF THE REPORT ....................................................................................................... 11 2 DEFINITION OF ENDOCRINE DISRUPTING CHEMICALS ...................................................................... 13 2.1 THE ENDOCRINE SYSTEM ............................................................................................................ 13 2.2 ADVERSITY ................................................................................................................................... 15 2.2.1 DEFINITION........................................................................................................................... 15 2.2.2 ASSAY REQUIREMENTS ........................................................................................................ 16 2.2.3 ECOTOXICOLOGICAL EFFECTS ............................................................................................. -
Compared Activity of Agonist Molecules Towards Ecdysone
PESTICIDES / SCIENTIFIC COMMUNICATION DOI: 10.1590/1808-1657000312019 Compared activity of agonist molecules towards ecdysone receptor in insect cell-based screening system Comparação da atividade de moléculas agonistas em relação ao receptor de ecdisona em um sistema de triagem baseado em linhagens celulares de insetos Ciro Pedro Guidotti Pinto1,2* , Letícia Neutzling Rickes2 , Moisés João Zotti2 , Anderson Dionei Grutzmacher2 ABSTRACT: The ecdysone receptor, naturally activated by ste- RESUMO: O receptor de ecdisona, naturalmente ativado por hor- roidal hormones, is a key protein for molting and reproduction mônios esteroidais, é uma proteína-chave nos processos de muda e processes of insects. Artificial activation of such receptor by specific reprodução de insetos. A ativação artificial desse receptor por meio de pesticides induces an anomalous process of ecdysis, causing death pesticidas específicos induz um processo de ecdise anômala, levando o of insects by desiccation and starvation. In this paper, we establi- inseto à morte por dessecação e inanição. Neste trabalho, foi estabele- shed a protocol for screening agonistic molecules towards ecdysone cido um protocolo para a triagem de moléculas agonistas em relação ao receptor of insect cells line S2 (Diptera) and Sf9 (Lepidoptera), receptor de ecdisona nas linhagens celulares responsivas S2 (Diptera) e transfected with the reporter plasmid ere.b.act.luc. Therefore, we Sf9 (Lepidoptera), transfectadas com o plasmídeo repórter ere.b.act.luc. set dose-response curves with the ecdysteroid 20-hydroxyecdysone, Para tanto, curvas de dose-resposta foram estabelecidas com o ecdiste- the phytoecdysteroid ponasterone-A, and tebufenozide, a pesticide roide 20-hidroxiecdisona, o fitoecdisteroide ponasterona-A e tebufeno- belonging to the class of diacylhydrazines. -
Binding Proteins for an Ecdysone Metabolite in the Crustacean Hepatopancreas (Molting Hormone/Steroid/Sucrose Gradient Oentrifugation) THOMAS A
Proc. Nat. Acad. Sci. USA Vol. 69, No. 4, pp. 812-815, April 1972 Binding Proteins for an Ecdysone Metabolite in the Crustacean Hepatopancreas (molting hormone/steroid/sucrose gradient oentrifugation) THOMAS A. GORELL*, LAWRENCE I. GILBERTt, AND JOHN B. SIDDALL Department of Biological Sciences, Northwestern University, Evanston, Illinois 60201; and Zoecon Research Corporation, Palo Alto, California 94304 Communicated by William S. Johnson, January 20, 1972 ABSTRACT When crustacean hepatopancreas is incu- and incubated at 250 in 3 ml of saline containing [8H]ecdysone bated in the presence of a-[3Hlecdysone of high specific (see figure legends for quantities). After incubation, the tissue activity and is then homogenized and centrifuged, a peak of protein-radioactivity is recovered after gel filtration of was rinsed twice in cold 0.1 M phosphate buffer (pH 7.3) and the 105,000 X g supernatant. Analysis of this peak by su- homogenized in buffer in a ground glass homogenizer at 4°. crose gradient centrifugation revealed the presence of two After centrifugation of the homogenate at 105,000 X g for 1.5 complexes of protein and labeled material (-11.5 S and hr at 40, the supernatant was concentrated with lyphogel and 6.35 S). The same results were obtained in vivo. On stand- for ing at low ionic strength, the lighter component disap- aliquots of the concentrated supernatant were assayed peared, suggesting that the heavier component is an aggre- protein (13) and radioactivity (Packard Tri-carb scintillation gate of the lighter one. Chemical analysis of radioactive ma- spectrometer, model 3375) in ethyl alcohol-toluene [2,5 di- terial in the complex revealed that it is not a- or j3-ecdysone phenyloxazole (PPO), 5 g/l; 1,4-bis-2-(4-methyl-5-phenoxa- nor any previously described metabolite of the ecdysones. -
U.S. EPA, Pesticide Product Label, , 08/21/1987
~ESTRICTED USE PESTICIDE D' ro ACUTE INHALATION TOXICITY OF h;~HLY TOXIC HYDROGEN PHOSPHIDE (PHOSPHINE, PH3) GAS For retail sale to and use only by certified applicators for those uses covered by the applicator's certification or persons trained in accordance with the Applicator's Manual working under the direct supervision and in the physical presence of the cartified applicator, Physical presence means on site or on the premises,. Read and follow the label and the DEGESCH America, Inc., Applicator's Manual which contains complllt&' instructions for the safe use of this pesticide: APPLICATOR'S MANUAL for DEGES~in· PELLETS and T ABLETS-R Patent No. 3132067 FOR USE AGAINST INSECTS WHICH INFEST STORED COMMODITIES AND CONTROL OF BURROWING PESTS Active Ingredient: Aluminum Phosphide ................................................ 55% Inert Ingredients ............... ........................................... , .. ' 45% KEEP OUT OF REACH OF CHILDREN DANGER - POISON - PELIGRO I PELiGRO AL USUARIO: Si usted no lee ingles, no use este producto hasta que la etiqueta se Ie haya sido explicado ampliamente. (TO THE USER: If you cannot read English, do not use this product until the label has been fully explained to you.) STATEMENT OF PRACTICAL TREATMENT Symptoms of overexposure are headache. dizziness, nausea, difficult breathing, vomiting, and diarrhea. In all cases of overexposure get medical attention immediately. Take victim to a doctor or emergency treatment facility. If the gas or dust from aluminum phosphide Is Inhaled: Get exposed person to fresh air. Keep warm and make sure person can breathe freely. If breathing has stopped, give artificial respiration by mouth-to-mouth or other means of resuscitation. Do not give anything by mouth to an unconscious person. -
Genetical and Physiological Mechanisms of Face Fly, Musca Autumnalis Degeer, Diapause Yonggyun Kim Iowa State University
Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1993 Genetical and physiological mechanisms of face fly, Musca autumnalis DeGeer, diapause Yonggyun Kim Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Biochemistry Commons, Entomology Commons, and the Genetics Commons Recommended Citation Kim, Yonggyun, "Genetical and physiological mechanisms of face fly, Musca autumnalis DeGeer, diapause " (1993). Retrospective Theses and Dissertations. 10245. https://lib.dr.iastate.edu/rtd/10245 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. -
Natural History)
A REVISION OF THE NODINI AND A KEY TO THE GENERA OF EUMOLPIDAE OF AFRICA (COLEOPTERA : EUMOLPIDAE) BY B. SELMAN I J. y Dept. of Agricultural Zoology, University, Newcastle-on-Tyne V_x Pp. 141-174 ; 27 Text-figures BULLETIN OF THE BRITISH MUSEUM (NATURAL HISTORY) ENTOMOLOGY Vol. 16 No. 3 LONDON: 1965 THE BULLETIN OF THE BRITISH MUSEUM (NATURAL HISTORY), instituted in 1949, is issued in five series corresponding to the Departments of the Museum, and an Historical Series. Parts will appear at irregular intervals as they become ready. Volumes will contain about three or four hundred pages, and will not necessarily be completed within one calendar year. In 1965 a separate supplementary series of longer papers was instituted, numbered serially for each Department. This paper is Vol. 16, No. 3 of the Entomological series. The abbreviated titles ofperiodicals cited follow those of the World List of Scientific Periodicals. Trustees of the British Museum (Natural History) 1965 TRUSTEES OF THE BRITISH MUSEUM (NATURAL HISTORY) Issued 23 August, 1965 Price Fourteen Shillings A REVISION OF THE NODINI AND A KEY TO THE GENERA OF EUMOLPIDAE OF AFRICA (COLEOPTERA : EUMOLPIDAE) By B. J. SELMAN CONTENTS Page INTRODUCTION ........... 143 THE TRIBAL CLASSIFICATION OF THE EUMOLPIDAE .... 144 REVISION OF THE TRIBE NODINI ........ 145 Redefinition of the genera of the tribe Nodini . .. .146 Index to the taxonomic changes in the tribe Nodini . .160 EUMOLPINI ........... 164 ADOXINI ............ 166 KEY TO THE GENERA OF THE EUMOLPIDAE OF AFRICA . .167 APPENDIX ...... .172 ACKNOWLEDGEMENTS ... ..... 173 REFERENCES ........... 173 SYNOPSIS The genera of the Eumolpidae of the Ethiopian region are revised and the tribes defined.