Carlos J. Esquivel Palma [email protected], phone: 330-347-7280 Department of Entomology, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691

PROFESSIONAL EXPERIENCE Graduate fellow, PhD. Candidate in Entomology, 2015–present, Department of Entomology, Ohio Agricultural Research and Development Center (OARDC), The Ohio State University, Wooster, OH. • Performing greenhouse and field experiments evaluating the durability of thiamethoxam in plant for the control of soybean aphid and green peach aphid. • Use of neonicotinoid seed treatment for management of soybean aphid virulence. • Toxicological tests via trophic food chain with thiamethoxam on beneficial insects. • Chemical quantification of insecticide residuals in plant tissue by the use of Ultra Performance Liquid Chromatography Mass Spectrometer. • Development of a novel technique to deliver small-interference RNA particles on aphids for gene knockdown. • Efficacy tests of insecticides to control western flower thrips and green peach aphid. • Rearing and colony maintenance of soybean aphids.

Graduate intern student, Summer 2016, Western Research Center, Dow AgroSciences, Fresno, CA. • Leading efficacy trials evaluating new compounds to control insects, fungi, and weeds. • Safety at work before, during, and after pesticide handling. • Development of bioassays to test insecticide efficacy under laboratory conditions. • Presenter at field tours organized at the research center.

Graduate research associate, MSc. in Entomology, 2013–2015, Department of Entomology, The Ohio State University, Columbus/Wooster, OH. • Development of a de novo transcriptome for the Malpighian tubules of the mosquito Aedes albopictus. • Development of a biochemical assay to measure the enzymatic activity of glutathione S-transferase of the Malpighian tubules of mosquitoes. • Bioinformatics for analysis of RNA sequencing data (mapping, alignment, assembly, annotation, BLAST). • Rearing and colony maintenance of Aedes aegypti, and Aedes albopictus mosquitoes. • RNA interference in mosquitoes by microinjection. • Biochemical assay to measure uric acid concentration in Malpighian tubules of mosquitoes. • Protein immunoblot trials for the detection of proteins in mosquito tissues. • Quantitative Real-Time PCR for gene expression quantification in insect tissues. • RNA isolation for conventional gene expression analysis and synthesis of cDNA genomic libraries.

Graduate teaching experience: • Fall, 2018. Lecturer for ‘Pesticides and their use’ at the Agricultural Technical Institute, The Ohio State University, Wooster, OH. Lectures were developed and taught to 26 undergraduate students. This course focuses to learn the safety, environmental costs, and use of insecticides, herbicides and fungicides for agricultural purposes. • Spring and Fall, 2017. Academic assistant at the Master in Plant Health Management Program, The Ohio State University. This program integrates classroom and e-learning resources for working professionals. I interacted with students from different states and backgrounds by answering questions related with assignments and facilitating the use of online learning tools. • Spring, 2016. Lecturer for ‘General and Applied Entomology’ at the Agricultural Technical Institute, The Ohio State University, Wooster, OH. Lectures and laboratories were developed and taught to 24 undergraduate students. This course focuses on general knowledge in entomology, biological roles of insects in the environment, and principles of pest management. • Spring, 2014. Teaching assistant for Agricultural Entomology. Laboratory with 30 undergraduate students. The Ohio State University, Columbus, OH. • Spring, 2014. Teaching assistant for Urban Entomology. Laboratory with 15 undergraduate students. The Ohio State University, Columbus, OH. • Fall, 2013. Teaching assistant for General Insect Pest Management. Laboratory with 20 undergraduate students. The Ohio State University, Columbus, OH. Undergraduate mentoring: • Summer 2017. Student Research Opportunities Program, The Ohio State University. Project: Tri-trophic effects on Orius insidiosus longevity post feeding on soybean aphid Aphis glycines with thiamethoxam accumulation by Raven Baxter, Dept. of Entomology, Wooster, OH. • Spring 2017. International OARDC-Zamorano University internship program (undergraduate student from Honduras). Project: Tri-trophic effects of thiamethoxam on Hippodamia convergens lady beetle when fed on Myzus persicae aphid exposed to treated Zinnia elegans by Erick Martinez, Dept. of Entomology, Wooster, OH. • Fall, 2014. International OARDC internship program (undergraduate student from Germany). Project: Protein immunoblot trials for the detection of V-type ATPase proteins in the Malpighian tubules of mosquitoes by Rosa Witty, Dept. of Entomology, Wooster, OH.

Visiting scholar, 2012–2013. Department of Entomology, OARDC, The Ohio State University, Wooster, OH. • RNA isolation and gene expression analysis in Malpighian tubules of mosquitoes. • Development of a protocol to rear and reproduce the exotic Asian tiger mosquito Aedes albopictus.

Research assistant, 2007. Guatemalan Sugar Cane Research Center, Guatemala. • Development of sugar cane cultivars adapted to Guatemala’s environmental conditions and pests by conventional breeding.

EDUCATION Ph.D. Candidate, 2015–Dec. 2019 (expected graduation). Department of Entomology, The Ohio State University, Wooster, OH. Current GPA: 3.73/4.00. Research statement: Molecular and toxicological interactions between thiamethoxam, aphids, and predatory natural enemies. M.Sc, Entomology, 2015, Department of Entomology, The Ohio State University, OH, GPA: 3.80/4.00. Thesis project: Effects of blood feeding on the transcriptome of the Malpighian tubules on the Asian tiger mosquito Aedes albopictus. B.Sc, major in Crop Science and Production, 2011, Escuela Agrícola Panamericana, Zamorano, Honduras. GPA: 3.79/4.00. Thesis project: Soil conservation plan at Zamorano farm, Honduras. Agronomist, minor in Crop Production, 2007, Escuela Nacional Central de Agricultura, Guatemala.

PUBLICATIONS Esquivel, C. J., Ranger, C., Phelan, L., Martinez, E., Hendrix, W. H., Cañas, L. A., & Michel, A. P. (2019). Weekly survivorship curves of soybean aphid biotypes 1 and 4 on insecticidal seed-treated soybean. Journal of Economic Entomology, 112(2): 712-719. Esquivel, C. J., Cassone, B. J., & Piermarini, P. M. (2016). A de novo transcriptome of the Malpighian tubules in non-blood- fed and blood-fed Asian tiger mosquitoes Aedes albopictus: insights into diuresis, detoxification, and blood meal processing. PeerJ, 4, e1784. http://doi.org/10.7717/peerj.1784 Esquivel, C. J., Cassone, B. J., & Piermarini, P. M. (2014). Transcriptomic evidence for dramatic functional transition of the Malpighian Tubules after a blood meal in the Asian tiger mosquito Aedes albopictus. Plos Negl Trop Dis. 8(6):1-16. Esquivel, C. J., Martinez, E., Baxter, R., Trabanino, R., Michel, A. P. & Cañas, L. A. (submitted). Thiamethoxam differentially impacts longevity of insidiosus flower bug and convergent lady beetle when exposed via the food chain. Journal of Pest Science. Sirot, L., Bansal, R., Esquivel, C. J., Arteaga, M., Herrera-Cruz, M., Corrêa Pavinato, V. A., Solana, A., Medel, K., Reyes- Hernández, M., Dorantes-Acosta, A., Michel, A., & Pérez-Staples, D. (in prep). Post-mating gene expression of Mexican fruit fly females: disentangling the effects of the male accessory glands. Journal of Insect Biochemistry and Molecular Biology. Parker, C., Bernaola, L., Elmquist, D., Cohen, A., Marshall, A., Hepler, J., Pekarcik, A., Justus, E., King, K., Lee, T., Esquivel C. J.,…& Skinner, R. (submitted). Entomology in the 21st Century: Tackling insect invasions, promoting advancement in technology, and using effective science communication – 2018 student debates. Journal of Insect Science. Piermarini, P., Esquivel, C. J., & Denton, J. (2017). Malpighian Tubules as Novel Targets for Mosquito Control. International Journal of Environmental Research and Public Health, 14(2), 111. http://doi.org/10.3390/ijerph14020111

Yang, Z., Statler, B.M., Calkins, T.L., Alfaro, E., Esquivel, C. J., Matthew, R.F., Denton, J., & Piermarini, P. (2017). Dynamic expression of genes encoding subunits of inward rectifier potassium (Kir) channels in the yellow fever mosquito Aedes aegypti, Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 204, February 2017, Pages 35-44, ISSN 1096-4959, https://doi.org/10.1016/j.cbpb.2016.11.003. Li, Y., Piermarini, P. M., Esquivel, C. J., Drumm, H. E., Schilkey, F. D., and Hansen, I. A. (2017). RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever Mosquito Aedes aegypti Reveals Life Stage-Specific Changes in Renal Function. Front. Physiol. 8: 1–13. Esquivel, C. J. (2018). Anatomy of a great entomological research poster. Entomology Today, Entomological Society of America. https://entomologytoday.org/2018/05/15/anatomy-of-a-great-entomological-research-poster/ Holt, J., Esquivel, C. J., Adesanya, A., and Whitener, A. (2018). The many facets of collaboration in entomological research and beyond. Entomology Today, Entomological Society of America. https://entomologytoday.org/2018/02/06/the- many-facets-of-collaboration-in-entomological-research-and-beyond/

AWARDS Presidential fellowship, 2019, Awarded by the Graduate School of The Ohio State University, Columbus, OH. 3rd place poster competition, 2019, Entomological North Central Branch meeting, March 18, Cincinnati, OH. 2nd place student debates, 2018, Entomological Society of America annual meeting, Nov. 13, BC, Canada. 3rd place oral presentation, 2018, Entomological North Central Branch meeting, March 20, Madison, WI. Student Travel Award, 2018, Entomological North Central Branch meeting, March 20, Madison, WI ($200). Research Scholarship, 2018. Ohio Soybean Council, March 13, Wooster, OH ($38,386). 1st place poster competition, 2017, 6th Symposium of Zamoranos in the USA, August 4, Gainesville, FL. 1st place oral presentation, 2017, Entomological Society of America annual meeting, Nov. 7, Denver, CO. 1st place oral presentation, 2017, Ohio Valley Entomological Association, Oct. 19, Columbus, OH. 1st place oral presentation, 2017, Entomological North Central Branch meeting, Jun. 6, Indianapolis, IN. 2nd place poster competition, 2017, 5th Symposium of Zamoranos in the USA, Oct. 22, Lubbock, TX. DeLong talk competition, 2016, 2017, awarded by the Dept. of Entomology, The Ohio State University, OH (US$1,350). Ray Travel Award for service, 2016, awarded by the CGS, The Ohio State University, OH (US$1,000). OARDC SEEDs Grant, 2016, awarded by the SEEDs Grants at OARDC, The Ohio State University, OH (US$5,000). 1st place poster competition, 2016, 4th Symposium of Zamoranos in the USA, Oct. 8, Baton Rouge, LA. 1st place poster competition, 2015, 3rd Symposium of Zamoranos in the USA, Sep. 26, Manhattan, KS. Lowell R. N. research award for ‘Esquivel et al., 2014’, 2015, Dept. of Entomology, The Ohio State University, OH. 2nd place poster competition (MSc), 2015, at the OARDC Annual Research Conference, The Ohio State University. 2nd place poster competition (MSc), 2014, at the OARDC Annual Research Conference, The Ohio State University. College scholarship, 2007, awarded by the Department of Agriculture, Guatemala (US$46,200).

HONORS Magna cum laude GPA, 2015, The Ohio State University, Columbus, OH. Summa cum laude GPA, 2011, Escuela Agrícola Panamericana, Zamorano, Honduras. Summa cum laude GPA, 2007, Escuela Nacional Central de Agricultura, Guatemala.

ASSOCIATION MEMBERSHIPS Member of the International Science, Technology, and Innovation Network of Guatemala, 2017–present. Member of the Ohio Valley Entomological Association, 2013–present. Member of the Entomology Graduate Student Association, The Ohio State University, 2013–present. Member of Entomological Society of America, 2013–present. Zamorano Alumni Association-U.S.A. Subchapter, 2012–present. Gamma Sigma Delta, Honor Society of Agriculture, 2010–present.

OUTREACH AND SERVICE President of the Entomology Graduate Student Association, The Ohio State University, OH, 2017–2018. Student representative, 2016–2018, P-IE Governing Council, Entomological Society of America. Student representative, 2016–2018, National Affairs Committee, Entomological Society of America. Organizer and moderator of symposiums at 2017 and 2018 Entomological Society of America annual meetings in Denver, CO and BC, Canada and at the 2017 North Central Branch meeting in Madison, WI. Reviewer at Insect Science Journal and Journal of Insect Physiology. Volunteer at the 2014, 2015, 2016, and 2017 Open House Museum Day. Museum of Biological Diversity, Columbus, OH. Volunteer at the 2014, 2015, 2016, 2017, 2018 and 2019 Bug’s Word in OARDC. Activities with elementary school children about insects. Volunteer at the 2014, 2015, 2016, 2017 and 2018 Insect Night Walk in OARDC. Interactive activities with elementary school children and parents about insects. Volunteer at the Entomological Society of America, North Central Branch and national meetings. President of the Zamorano Alumni Association-Ohio Subchapter, 2012–2013. Volunteer at the Program Center, 2011, Holit kibbutz, , .

SKILLS AND PERSONAL VALUES Leadership; team worker; outstanding written and verbal communication skills; bilingual and fluent in English and Spanish; mentoring experience; analytical thinking; goal-oriented; creative; problem solving skills; honesty; adaptability; dedication; self-motivated; environmentally ethical.