Nachwuchswissenschaftlerforum / Young Scientists Meeting 2012 Philippi et al. Penetration behavior of different species on angustifolius L. genotypes

Philippi, J. 1, Schliephake, E. 1, Ordon, F. 1

1 Julius Kuehn-Institute, Institute for Resistance Research and Stress Tolerance Email of corresponding author: [email protected]

Due to the high level of protein angustifolius L., and the polyphagous content in the seeds of up to 40%, the aphid species green peach aphid ability to fix nitrogen by symbiosis (Myzus persicae) and black bean aphid with rhizobia and the deep root (Aphis fabae). system improving soil structure and A method to record the penetration quality, lupin cultivation is of growing behavior of is the Electrical interest. Wild types of lupins Penetration Graph (EPG). Aphids are accumulate alkaloids which are toxic fixed on a thin gold wire by a droplet metabolites working as deterrents for of silver glue. The conductivity of this several pests and diseases. A high attachment allows carrying low content of alkaloids prohibits the use current through the aphid when stylet of lupins in human and diets. penetration occurs. The wired aphids Breeding of cultivars with a low produce an electrical signal during the content of alkaloids (“sweet lupins”) in penetration progress. Depending on the 1930’s enhanced the use of lupin the penetrated plant tissue and the seeds for human and animal nutrition, different parts of penetration but led to a higher susceptibility to e.g. procedure, e.g. salivation or phloem different aphids. Aphids harm plants sap ingestion, the recorded by penetrating the cells, feeding on waveforms of wired aphids show the phloem sap, leading to an different patterns. inhibition of plant growth and by The aim of the test is to analyze, how serving as vectors of different viruses. far the penetration behavior of the Three genotypes of blue lupin ( Lupinus well adapted lupin aphid is different angustifolius L.) with different levels of from that of the polyphagous green alkaloid concentrations - the sweet peach and black bean aphid on bitter genotypes “Boregine” and and sweet lupin genotypes. Results “Bo083521AR” with a low content of obtained up to now give hint that the alkaloids, and the bitter, alkaloid rich lupin aphid is unaffected by the genotype “Azuro” - were evaluated alkaloid level while the green peach with regard to the penetration and the black bean aphid show an behavior of the lupin aphid alkaloid-level depending penetration ( albifrons), which is well behavior. adapted to alkaloids in Lupinus

45 Berichte aus dem Julius Kühn-Institut 167 . 2012