DOCSLIB.ORG

The Neurofilament-Derived Peptide NFL-TBS.40-63 Targets Neural Stem Cells and Affects Their Properties

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Neurofilament-Derived Peptide NFL-TBS.40-63 Targets Neural Stem Cells and Affects Their Properties Cell-Based Drug Development, Screening, and Toxicology CELL-BASED DRUG DEVELOPMENT,SCREENING, AND TOXICOLOGY The Neurofilament-Derived Peptide NFL-TBS.40-63 Targets Neural Stem Cells and Affects Their Properties * * CLAIRE LEPINOUX´ -CHAMBAUD, KRISTELL BARREAU, JOEL¨ EYER Key Words. Neural stem cells x NFL-TBS.40-63 peptide x Targeting x Subventricular zone x Differentiation x Proliferation Laboratoire Neurobiologie ABSTRACT et Transgenese, L’Universite´ Targeting neural stem cells (NSCs) in the adult brain represents a promising approach for developing Nantes, Angers, Le Mans, new regenerative strategies, because these cells can proliferate, self-renew, and differentiate into new Unite´ Propre de Recherche neurons, astrocytes, and oligodendrocytes. Previous work showed that the NFL-TBS.40-63 peptide, de l’Enseignement Superieur´ corresponding to thesequenceof a tubulin-binding site on neurofilaments,can target glioblastomacells, EA-3143, Institut de Biologie where it disrupts their microtubules and inhibits their proliferation. We show that this peptide targets en Sante,´ Universite´ NSCsinvitro and invivo when injected intothe cerebrospinalfluid. Although neurosphere formation was not altered by the peptide, the NSC self-renewal capacity and proliferation were reduced and were d’Angers, Centre Hospitalier associated with increased adhesion and differentiation. These results indicate that the NFL-TBS.40-63 Universitaire, Angers, France peptide represents a new molecular tool to target NSCs to develop new strategies for regenerative med- STEM CELLS TRANSLATIONAL MEDICINE – *Contributed equally. icine and the treatment of brain tumors. 2016;5:901 913 Correspondence: Joel¨ Eyer, Ph.D., Laboratoire Neurobiologie & Transgenese, L’UNAM, UPRES SIGNIFICANCE EA-3143, Institut de Biologie en In the present study, the NFL-TBS.40-63 peptide targeted neural stem cells in vitro when isolated from Sante,´ Universited´ ’Angers, Centre Hospitalier Universitaire, the subventricular zone and in vivo when injected into the cerebrospinal fluid present in the lateral Batimentˆ IBS-IRIS, 49033 Angers, ventricle. The in vitro formation of neurospheres was not altered by the peptide; however, at a high France. Telephone: 02-44-68-84-88. concentration of the peptide, the neural stem cell (NSC) self-renewal capacity and proliferation were E-Mail: [email protected] reduced and associated with increased adhesion and differentiation. These results indicate that the Received August 27, 2015; NFL-TBS.40-63 peptide represents a new molecular tool to target NSCs to develop new strategies for accepted for publication regenerative medicine and the treatment of brain tumors. February 23, 2016; published Online First on May 13, 2016. ©AlphaMed Press INTRODUCTION lesions, and malignant glioma [12, 13]. Several 1066-5099/2016/$20.00/0 studies have also showed the recruitment of http://dx.doi.org/ It is a major challenge to treat chronic diseases of endogenousNSCsinseveralbraindisorders.For in- 10.5966/sctm.2015-0221 the central nervous system, because most active stance, in patients with multiple sclerosis, endog- drugs do not pass the blood-brain barrier efficiently enous mobilization of NSCs from the SVZ to to allow sufficient bioavailability amounts in the striatal lesions was observed [14]. Endogenous mi- brain. Another important issue is to deliver the gration of NSCs to the glioma was also demon- highlybioactive moleculesspecificallyto thedesired strated [15]. A recent review of clinical trials site of action to avoid secondary or toxic effects. reported the use of NSCs as a possible treatment One promising strategy is to inject such molecules of neurodegenerative diseases, including amyo- in the cerebrospinal fluid to target neural stem cells trophic lateral sclerosis, stroke, and spinal cord in- (NSCs). These cells are located in the neurogenic re- jury [16]. Therefore, the capacity to target and gions of the adult brain (subventricular zone [SVZ], manipulate endogenous NSCs represents a promis- dentate gyrus of the hippocampus) and along the ing avenue for regenerative-based therapies to in- central canal of the spinal cord [1–8]. The SVZ rep- crease their mobilization, stimulate neurogenesis, resents the most important pool of endogenous and avoid the occurrence of side effects [17]. NSCs in the brain. They are characterized by their A potential candidate to target NSCs is the capacity to self-renew, form neurospheres in cul- NFL-TBS.40-63 peptide, which corresponds to the ture, proliferate, and differentiate into neurons, as- tubulin-binding site located on the light subunit trocytes, and oligodendrocytes [9–11]. The use of of neurofilaments [18]. The peptide alone, as well normal or genetically modified NSCs has been de- as nanoparticles functionalized with the peptide, scribed for the treatment of neurodegenerative dis- can massively enter glioblastoma cells in vitro orders (Parkinson’s, Huntington’s, and Alzheimer’s and in vivo; however, the uptake is very low in diseases, amyotrophic lateral sclerosis), spinal cord healthy cells (astrocytes and neurons) [19–21]. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:901–913 www.StemCellsTM.com ©AlphaMed Press 2016 902 NFL-TBS.40-63 Peptide Targets Neural Stem Cells Moreover, this peptide disrupts the microtubule network of glio- 20 ng/ml EGF. The cells were incubated in the absence or presence blastoma cells and reduces the tumor volume in animals bearing of increasing concentrations of biotinylated NFL-TBS.40-63 peptide glioma but has no major toxicity on healthy cells. The peptide or with 1 mg/ml colchicine (Sigma-Aldrich). After 5–7 days, the total did not damage the tissue when injected into healthy brain number of primary neurospheres and the percentage of adherent [19–21]. Recent works have also shown that this peptide promotes primary neurospheres were determined in each condition using mi- the differentiation and maturation of oligodendrocytes derived croscope analysis. For the self-renewal assay, primary neurospheres from newborn rats in vitro [22, 23]. were collected in each condition, dissociated into single cells, and In the present study, we investigated the targeting capacity and seeded in media containing 20 ng/ml EGF. After 5 days, the total potential effects of this peptide on NSCs. We show that the peptide is number of secondary neurospheres was counted in each condition able to target NSCs isolated from newborn and adult animals in vitro using microscopeanalysis. The morphologyofprimaryneurospheres and in vivo after its injection into the lateral ventricle of adult rats. was observed with an inverted microscope (Leica DMI6000; Leica Although the peptide showed a cytotoxic effect on the glioblastoma Biosystems, Nussloch, Germany, http://www.leicabiosystems.com) cells, it has no major effect on NSCs at concentrations less than equipped with a CoolSNAP-HQ2 camera and analyzed using 10 mmol/l. At higher concentrations, the peptide affects their self- MetaMorph, version 7.1.7.0, software (Molecular Devices, renewal and proliferation ability and increases their adhesion and dif- Sunnyvale, CA, http://www.moleculardevices.com). They were ferentiation. Together, these results indicate that the NFL-TBS.40-63 also observed using scanning electron microscopy. peptide represents a promising tool for targeting the delivery of bi- ologically active proteins into NSCs and/or manipulating these cells. Flow Cytometry (Fluorescence-Activated Cell Sorting) The uptake of 5-FAM-labeled NFL-TBS.40-63 and NFL-SCR pep- MATERIALS AND METHODS tides was quantified in NSCs derived from newborn and adult rats. Cell Culture and Materials A total of 200–300 neurospheres were seeded in 35-mm dishes and cultured for 30 minutes at 37°C in media containing 5-FAM- Primary cultures of NSCs were derived from the SVZ of newborn labeled peptides. To quench the extracellular signal of the 5-FAM- (1–5days)andadult(,4 months) Wistar rats, as previously de- labeled peptides, 0.4% trypan blue (Sigma-Aldrich) was added scribed [24]. The dissociated cells were grown in minimal essential before analysis. To investigate the uptake molecular mechanism, medium-a (Thermo Fisher Scientific Life Sciences, Waltham, MA, neurospheres were preincubated at 4°C for 30 minutes or with http://www.thermofisher.com) supplemented with 25 mmol/l 10 mmol/l sodium azide in the presence of 6mmol/l 2-deoxy-D-glucose D-glucose (Sigma-Aldrich, St. Louis, MO, http://www.sigmaaldrich. to deplete cellular ATP or with different endocytosis and signaling com), 1 mmol/l sodium pyruvate, 15 mmol/l HEPES, 5% penicillin/ pathway inhibitors for 30 minutes at 37°C, as previously described streptomycin, 1% B27 (Thermo Fisher Scientific Life Sciences), and [21]. Next, 20 mmol/l 5-FAM-labeled NFL-TBS.40-63 peptide was 20 ng/ml epidermal growth factor (EGF; Promega, Madison, WI, added to the cells for 30 minutes at 37C°. Subsequently, after centri- http://www.promega.com). After 5–7 days, the stem cells formed fugation (5 minutes at 42g), the cells were dissociated mechanically floating neurospheres. andwashedtwicewithPBSandthenresuspendedin50mg/ml propi- The NFL-TBS.40-63 peptide (YSSYSAPVSSSLSVRRSYSSSSGS) is dium iodide (PI; Sigma-Aldrich). The fluorescent-positive viable cells biotinylated or coupled to 5-carboxyfluorescein (5-FAM) and was that incorporated the 5-FAM-labeled peptide were analyzed by flow synthesized by Millegen (Toulouse, France), Eurogentec (Seraing, cytometry (FACSCalibur; Becton Dickinson, Franklin Lakes, NJ, http:// Belgium, http://www.eurogentec.com),
Load more

Recommended publications
  • The Pennsylvania State University
    The Pennsylvania State University The Graduate School Department of Neural and Behavioral Sciences A REGENERATIVE RESPONSE OF ENDOGENOUS NEURAL STEM CELLS TO PERINATAL HYPOXIC/ISCHEMIC BRAIN DAMAGE A Thesis in Neuroscience by Ryan J. Felling © 2006 Ryan J. Felling Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy May 2006 ii The thesis of Ryan J. Felling was reviewed and approved* by the following: Steven W. Levison Professor of Neurology and Neurosciences Thesis Advisor Co-Chair of Committee Teresa L. Wood Associate Professor of Neural and Behavioral Sciences Co-Chair of Committee Sarah K. Bronson Assistant Professor of Cell and Molecular Biology Charles Palmer Professor of Pediatrics James R. Connor Professor and Vice-Chair Department of Neurosurgery; Director, G.M. Leader Family Laboratory for Alzheimer's Disease Research Robert J. Milner Professor of Neural and Behavioral Sciences Head of Neuroscience Graduate Program *Signatures are on file in the Graduate School iii ABSTRACT Hypoxic/ischemic (H/I) insults are the leading cause of neurologic injury during the perinatal period, affecting 2-4 per 1000 term births as well as a high percentage of premature infants. The ensuing sequelae are devastating and include cerebral palsy, epilepsy and cognitive deficits. Despite astounding advances in perinatal care, the incidence of cerebral palsy has changed little over the last 50 years. This demands that we pursue alternative therapeutic strategies that will reduce the significant morbidity associated with perinatal H/I encephalopathy. The revelation that the brain retains populations of neural stem cells throughout life offers the promise of endogenous regeneration following brain injury.
    [Show full text]
  • Evidence for Newly Generated Interneurons in the Basolateral Amygdala of Adult Mice
    OPEN Molecular Psychiatry (2018) 23, 521–532 www.nature.com/mp ORIGINAL ARTICLE Evidence for newly generated interneurons in the basolateral amygdala of adult mice DJ Jhaveri1,2,5, A Tedoldi1,5, S Hunt1, R Sullivan1, NR Watts3, JM Power4, PF Bartlett1,6 and P Sah1,6 New neurons are continually generated from the resident populations of precursor cells in selective niches of the adult mammalian brain such as the hippocampal dentate gyrus and the olfactory bulb. However, whether such cells are present in the adult amygdala, and their neurogenic capacity, is not known. Using the neurosphere assay, we demonstrate that a small number of precursor cells, the majority of which express Achaete-scute complex homolog 1 (Ascl1), are present in the basolateral amygdala (BLA) of the adult mouse. Using neuron-specific Thy1-YFP transgenic mice, we show that YFP+ cells in BLA-derived neurospheres have a neuronal morphology, co-express the neuronal marker βIII-tubulin, and generate action potentials, confirming their neuronal phenotype. In vivo, we demonstrate the presence of newly generated BrdU-labeled cells in the adult BLA, and show that a proportion of these cells co-express the immature neuronal marker doublecortin (DCX). Furthermore, we reveal that a significant proportion of GFP+ neurons (~23%) in the BLA are newly generated (BrdU+) in DCX-GFP mice, and using whole-cell recordings in acute slices we demonstrate that the GFP+ cells display electrophysiological properties that are characteristic of interneurons. Using retrovirus-GFP labeling as well as the Ascl1CreERT2 mouse line, we further confirm that the precursor cells within the BLA give rise to mature and functional interneurons that persist in the BLA for at least 8 weeks after their birth.
    [Show full text]
  • Cerebellum- and Forebrain-Derived Stem Cells Possess Intrinsic Regional Character Corinna Klein1, Simon J
    Research article 4497 Cerebellum- and forebrain-derived stem cells possess intrinsic regional character Corinna Klein1, Simon J. B. Butt1, Robert P. Machold1, Jane E. Johnson2 and Gord Fishell1,* 1Developmental Genetics Program and the Department of Cell Biology, The Skirball Institute of Biomolecular Medicine, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA 2Center for Basic Neuroscience, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9111, USA *Author for correspondence (e-mail: fi[email protected]) Accepted 10 August 2005 Development 132, 4497-4508 Published by The Company of Biologists 2005 doi:10.1242/dev.02037 Summary The existence of stem cells in the adult nervous system is regard specifically to embryonic and adult cerebellar stem well recognized; however, the potential of these cells is still cells, we observe that they are able to give rise to neurons widely debated. We demonstrate that neural stem cells exist that resemble different select classes of cerebellar within the embryonic and adult cerebellum. Comparing the subclasses when grafted into the perinatal host cerebellum. potential of neural stem cells derived from the forebrain Most notably, upon transplantation to the perinatal and cerebellum, we find that progeny derived from each of cerebellum, cerebellar stem cells from all ages are able to these brain regions retain regional character in vitro as well acquire the position and mature electrophysiological as after homotopic transplantation. However, when properties of cerebellar granule cells. ectopically transplanted, neurosphere-derived cells from either region are largely unable to generate neurons. With Key words: Cerebellum, Neural stem cell, Forebrain, Mouse Introduction (Clarke and Frisen, 2001; D’Amour and Gage, 2002).
    [Show full text]
  • Stochastic Cellular Automata Model of Tumorous Neurosphere Growth: Roles of Developmental Maturity and Cell Death
    Journal of Theoretical Biology 467 (2019) 100–110 Contents lists available at ScienceDirect Journal of Theoretical Biology journal homepage: www.elsevier.com/locate/jtb Stochastic cellular automata model of tumorous neurosphere growth: Roles of developmental maturity and cell death ∗ Günther K.H. Zupanc a, , Frederick B. Zupanc a, Rifat Sipahi b a Laboratory of Neurobiology, Department of Biology, Northeastern University, Boston, MA, USA b Complex Dynamic Systems and Control Laboratory, Department of Mechanical and Industrial Engineering, Northeastern University, Boston, MA, USA a r t i c l e i n f o a b s t r a c t Article history: The neurosphere assay is a powerful in vitro system for studying stem/progenitor-cell-driven tissue Received 10 July 2018 growth. By employing a stochastic cellular automata model, we simulated the development of tumorous Revised 13 December 2018 neurospheres in response to transformation of a randomly selected progenitor cell into a brain tumor Accepted 19 January 2019 stem cell. Simulated tumorous neurospheres were distinguished from normal neurospheres by their Available online 29 January 2019 size, which exceeded that of normal neurospheres typically manifold. A decisive factor that determined Keywords: whether brain tumor stem cells gave rise to tumorous neurospheres was their ability to escape en- Neural stem/progenitor cells capsulation by neighboring cells, which suppressed mitotic activity through contact inhibition. In our Brain tumor stem cells simulations, the likelihood of tumorigenesis was strongly negatively correlated with the developmental Neurosphere assay maturity of the neurospheres in which the transformation of a progenitor cell into a brain tumor stem Tumor growth cell was induced.
    [Show full text]
  • Endogenous Interferon Directly Regulates Neural Precursors in The
    9038 • The Journal of Neuroscience, July 7, 2010 • 30(27):9038–9050 Development/Plasticity/Repair Endogenous Interferon ␥ Directly Regulates Neural Precursors in the Non-Inflammatory Brain Li Li, Tara L. Walker, Ying Zhang, Eirinn W. Mackay, and Perry F. Bartlett Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia Although a number of growth factors have been shown to be involved in neurogenesis, the role of inflammatory cytokines remains relatively unexplored in the normal brain. Here we investigated the effect of interferon gamma (IFN␥) in the regulation of neural precursor (NP) activity in both the developing and the adult mouse brain. Exogenous IFN␥ inhibited neurosphere formation from the wild-type neonatal and adult subventricular zone (SVZ). More importantly, however, these effects were mirrored in vivo, with mutant mice lacking endogenous IFN␥ displaying enhanced neurogenesis, as demonstrated by an increase in proliferative bromodeoxyuridine- labeled cells in the SVZ and an increased percentage of newborn neurons in the olfactory bulb. Furthermore, NPs isolated from IFN␥ null mice exhibited an increase in self-renewal ability and in the capacity to produce differentiated neurons and oligodendrocytes. These effects resulted from the direct action of IFN␥ on the NPs, as determined by single-cell assays and the fact that nearly all the neurospheres werederivedfromcellspositiveformajorhistocompatibilitycomplexclassIantigen,adownstreammarkerofIFN␥-mediatedactivation. Moreover,theinhibitoryeffectwasamelioratedinthepresenceofSVZ-derivedmicroglia,withtheirremovalresultinginalmostcomplete inhibition of NP proliferation. Interestingly, in contrast to the results obtained in the adult, exogenous IFN␥ treatment stimulated neurosphere formation from the embryonic brain, an effect that was mediated by sonic hedgehog. Together these findings provide the first direct evidence that IFN␥ acts as a regulator of the active NP pool in the non-inflammatory brain.
    [Show full text]
  • In Vivo Conversion of Astrocytes to Neurons in the Injured Adult Spinal Cord
    ARTICLE Received 2 Jan 2014 | Accepted 29 Jan 2014 | Published 25 Feb 2014 DOI: 10.1038/ncomms4338 In vivo conversion of astrocytes to neurons in the injured adult spinal cord Zhida Su1,2, Wenze Niu1, Meng-Lu Liu1, Yuhua Zou1 & Chun-Li Zhang1 Spinal cord injury (SCI) leads to irreversible neuronal loss and glial scar formation, which ultimately result in persistent neurological dysfunction. Cellular regeneration could be an ideal approach to replenish the lost cells and repair the damage. However, the adult spinal cord has limited ability to produce new neurons. Here we show that resident astrocytes can be converted to doublecortin (DCX)-positive neuroblasts by a single transcription factor, SOX2, in the injured adult spinal cord. Importantly, these induced neuroblasts can mature into synapse-forming neurons in vivo. Neuronal maturation is further promoted by treatment with a histone deacetylase inhibitor, valproic acid (VPA). The results of this study indicate that in situ reprogramming of endogenous astrocytes to neurons might be a potential strategy for cellular regeneration after SCI. 1 Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390-9148, USA. 2 Institute of Neuroscience and Key Laboratory of Molecular Neurobiology of Ministry of Education, Neuroscience Research Center of Changzheng Hospital, Second Military Medical University, 800 Xiangyin Rd, Shanghai 200433, China. Correspondence and requests for materials should be addressed to C.-L.Z. (email: [email protected]).
    [Show full text]
  • Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells in Vitro
    International Journal of Molecular Sciences Article Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells In Vitro Alejandro Herrera 1, Sara Morcuende 1 , Rocío Talaverón 2 , Beatriz Benítez-Temiño 1 , Angel M. Pastor 1 and Esperanza R. Matarredona 1,* 1 Departamento de Fisiología, Facultad de Biología, Universidad de Sevilla, 41012 Seville, Spain; [email protected] (A.H.); [email protected] (S.M.); [email protected] (B.B.-T.); [email protected] (A.M.P.) 2 Instituto de Neurociencias de Castilla y Léon (INCYL), Universidad de Salamanca, 37007 Salamanca, Spain; [email protected] * Correspondence: [email protected]; Tel.: +34-955-420-279 Abstract: Neural progenitor cells (NPCs) are self-renewing and multipotent cells that persist in the postnatal and adult brain in the subventricular zone and the hippocampus. NPCs can be expanded in vitro to be used in cell therapy. However, expansion is limited, since the survival and proliferation of adult NPCs decrease with serial passages. Many signaling pathways control NPC survival and renewal. Among these, purinergic receptor activation exerts differential effects on the biology of adult NPCs depending on the cellular context. In this study, we sought to analyze the effect of a general blockade of purinergic receptors with suramin on the proliferation and survival of NPCs isolated from the subventricular zone of postnatal rats, which are cultured as neurospheres. Treatment of neurospheres with suramin induced a significant increase in neurosphere diameter and in NPC number attributed to a decrease in apoptosis. Proliferation and multipotency were not affected. Citation: Herrera, A.; Morcuende, S.; Suramin also induced an increase in the gap junction protein connexin43 and in vascular endothelial Talaverón, R.; Benítez-Temiño, B.; growth factor, which might be involved in the anti-apoptotic effect.
    [Show full text]
  • Local and Global Spontaneous Calcium Events Regulate Neurite Outgrowth and Onset of Gabaergic Phenotype During Neural Precursor Differentiation
    The Journal of Neuroscience, January 1, 2003 • 23(1):103–111 • 103 Local and Global Spontaneous Calcium Events Regulate Neurite Outgrowth and Onset of GABAergic Phenotype during Neural Precursor Differentiation Francesca Ciccolini,1,2 Tony J. Collins,1 Juliana Sudhoelter,1 Peter Lipp,1 Michael J. Berridge,1 and Martin D. Bootman1 1Laboratory of Molecular Signalling, The Babraham Institute, Babraham, Cambridge CB2 4AT, United Kingdom, and 2Cambridge Centre for Brain Repair, Cambridge University, Forvie Site, Cambridge CB2 2PY, United Kingdom Neural stem cells can generate in vitro progenitors of the three main cell lineages found in the CNS. The signaling pathways underlying the ϩ acquisition of differentiated phenotypes in these cells are poorly understood. Here we tested the hypothesis that Ca2 signaling controls ϩ differentiation of neural precursors. We found low-frequency global and local Ca 2 transients occurring predominantly during early stages of ϩ ϩ differentiation. Spontaneous Ca 2 signals in individual precursors were not synchronized with Ca 2 transients in surrounding cells. Experi- ϩ ϩ mentallyinducedchangesinthefrequencyoflocalCa 2 signalsandglobalCa 2 risescorrelatedpositivelywithneuriteoutgrowthandtheonset of GABAergic neurotransmitter phenotype, respectively. NMDA receptor activity was critical for alterations in neuronal morphology but not for ϩ the timing of the acquisition of the neurotransmitter phenotype. Thus, spontaneous Ca 2 signals are an intrinsic property of differentiating neurosphere-derived precursors. Their frequency may specify neuronal morphology and acquisition of neurotransmitter phenotype. Key words: neural precursors; neuronal differentiation; calcium signaling; neurospheres; NMDA receptors; GABA Introduction cursors. This highlights the importance of investigating the Neurons and glial cells, the main cell types of the mammalian mechanisms responsible for neuronal specification in this CNS, arise from a common stem cell (for review, see McKay, system.
    [Show full text]
  • The Predominant Neural Stem Cell Isolated from Postnatal and Adult Forebrain but Not Early Embryonic Forebrain Expresses GFAP
    2824 • The Journal of Neuroscience, April 1, 2003 • 23(7):2824–2832 The Predominant Neural Stem Cell Isolated from Postnatal and Adult Forebrain But Not Early Embryonic Forebrain Expresses GFAP Tetsuya Imura,1 Harley I. Kornblum,2,3 and Michael V. Sofroniew1 Departments of 1Neurobiology, 2Pharmacology, and 3Pediatrics, and Brain Research Institute, University of California, Los Angeles, California 90095-1763 Periventricular germinal zones (GZs) of developing and adult brain contain neural stem cells (NSCs), the cellular identities and origins of which are not defined completely. We used tissue culture techniques and transgenic mice expressing herpes simplex virus thymidine kinase (HSV-TK) from the mouse glial fibrillary acid protein (GFAP) promoter to test the hypothesis that certain NSCs express GFAP. To do so, we determined the relative proportions of multipotent neurospheres that are formed by GFAP-expressing cells derived from GZs at different stages of development. In this transgenic model, dividing GFAP-expressing cells are ablated selectively by treatment with the antiviral agent ganciclovir (GCV). Single-cell analysis showed that transgene-derived HSV-TK was present only in GFAP-expressing cells. GCV applied in vitro eliminated growth of multipotent neurospheres from GZs of postnatal and adult transgenic mice but not early embryonic (embryonic day 12.5) transgenic mice. GCV prevented growth of secondary multipotent neurospheres prepared after passage of primary transgenic neurospheres derived from all three of these developmental stages. In addition, GCV prevented growth of multi- potent neurospheres from transgenic astrocyte-enriched cell cultures derived from postnatal GZ, and elaidic acid GCV given for4dto adult transgenic mice in vivo abolished the ability to grow multipotent neurospheres from GZ.
    [Show full text]
  • Neural Stem Cells Standardized Media and Reagents
    NEURAL STEM CELL PRODUCTS Neural Stem Cells Standardized Media and Reagents Table of Contents Table of Contents 3 Superior Neural Stem Cell Culture with Consistent, High-Quality Media and Reagents 4 Culture Mouse Neural Stem Cells 6 Culture Rat Neural Stem Cells 8 Culture Human Neural Stem Cells 10 Identify and Enumerate Neural Stem Cells 12 Passage Neural Stem Cells 13 Dissociate CNS Tissue 13 Differentiate Human Pluripotent Stem Cells to Neural Progenitors 14 Additional Products For Neural Cell Research 14 References Scientists Helping Scientists STEMCELL Technologies has been setting standards for quality cell culture media and reagents since 1993. As scientists helping scientists, we have developed over 1000 specialty products for stem cells from a variety of tissues, including neural, hematopoietic, pluripotent, mesenchymal, mammary and more. The NeuroCultTM product line includes nearly 30 different media products, culture assays and differentiation kits for primary mammalian neural stem cells, as well as media supplements for neurons. Learn from NeuroCultTM protocols, educational videos, webinars and mini-reviews at www.stemcell.com. 22 NEURAL STEM CELL PRODUCTS Superior Neural Stem Cell Culture With Consistent, High-Quality Media and Reagents Neural stem cells (NSCs) are defined as cells with the ability to proliferate, self-renew, and produce a large number of functional progeny that can differentiate into neurons, astrocytes and oligodendrocytes. They were first Advantages of NeuroCult™ isolated from the embryonic and adult mouse striatum in for Neural Stem Cells the early 1990s using the neurosphere assay,1,2 and have since been identified in nearly all regions of the embryonic STANDARDIZED. Standardized media and culture mouse, rat and human CNS as well as the subgranular conditions minimize variability and lead to increased 3 layer of the dentate gyrus in the mature CNS.
    [Show full text]
  • Identification of a Multipotent Astrocytic Stem Cell in the Immature and Adult Mouse Brain
    Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain Eric D. Laywell*†, Pasko Rakic‡, Valery G. Kukekov*, Eric C. Holland§, and Dennis A. Steindler*† *Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee Health Science Center, 855 Monroe Avenue, Memphis, TN 38163; ‡Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510; and §Department of Neurosurgery, M.D. Anderson Cancer Center, Houston, TX 77030 Contributed by Pasko Rakic, October 2, 2000 The mammalian brain contains a population of neural stem cells cultured in conditions that cause them to pass through a state of (NSC) that can both self-renew and generate progeny along the differentiation as type 2 astrocytes (10). three lineage pathways of the central nervous system (CNS), but To study the identity of the NSC (11), we use clonogenic the in vivo identification and localization of NSC in the postnatal culture conditions, and a transgenic mouse that allows for the CNS has proved elusive. Recently, separate studies have implicated analysis of the progeny of cells within the astrocyte lineage, to ciliated ependymal (CE) cells, and special subependymal zone (SEZ) examine the ability of CE cells and astrocytes to form multipo- astrocytes as candidates for NSC in the adult brain. In the present tent neurospheres. We demonstrate a ‘‘global’’ ability for astro- study, we have examined the potential of these two NSC candi- cytes from the cerebral cortex, cerebellum, spinal cord, and SEZ dates to form multipotent spherical clones—neurospheres—in grown as monolayers to form neurospheres that can assume vitro.
    [Show full text]
  • Structure and Ultrastructure of Neurospheres Spontaneously Generated in Culture from Sheep Ovarian Cortical Cells
    Structure and ultrastructure of neurospheres spontaneously generated in culture from sheep ovarian cortical cells. Concepción Rojo Salvador Universidad Complutense de Madrid Facultad de Veterinaria Belén Sánchez-Maldonado Universidad Complutense de Madrid Facultad de Veterinaria María de Lourdes Galicia Universidad Complutense de Madrid Facultad de Veterinaria Alfredo González-Gil Universidad Complutense de Madrid Facultad de Veterinaria Rosa Ana Picazo González ( [email protected] ) Universidad Complutense de Madrid Facultad de Veterinaria Research article Keywords: Spheroid, Neurosphere, Light cells, Dark cells, Ovarian cortical cells, Neural stem cells, Neural progenitors Posted Date: December 6th, 2019 DOI: https://doi.org/10.21203/rs.2.18012/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/30 Abstract Background: Neurospheres derived from adult stem cells of non-neural tissues represent a promising source of neural stem cells (NSCs) and neural progenitor cells (NPCs) for autologous cell therapy in neurological diseases. Analyzing the ne structure of neurospheres can provide pivotal information regarding the phenotype of spheroid-integrating cells, being useful for cell characterization during differentiation, and oncogenic transformation. Neurospheres can be generated by culturing ovarian cortical cells under particular conditions. In order to assess the reliability of use of these spheroids in regenerative medicine, developmental biology, and to state whether they share morphological features with central nervous system (CNS) derived neurospheres, they were analyzed by light and transmission electron microscopy. Sheep ovarian cortical cells were cultured in serum-free medium for 21 days to generate neurospheres. On days 10, 15 and 21 of culture, expression of pluripotency (Nanog, Oct4, Sox2), and NSC/NPC (nestin, Pax6, P75NTR) transcripts by qRT-PCR, immunolocalization of NSC/NPC antigens (nestin, Pax6, P75NTR), structure, and ultrastructure, were analyzed in spheroids.
    [Show full text]