Scyllatoxin recombinant, expressed in E. coli

Catalog Number S 2321

Synonyms: Leiurotoxin I; LeTx I Preparation Instructions A stock solution of 1 µM can be obtained by adding Product Description 0.292 ml of any conventional buffer per µg of peptide. Scyllatoxin, recombinant, with the sequence AFCNLRMCQL SCRSLGLLGK CIGDKCECVK H, is Storage/Stability expressed in and extracted from E. coli and purified to Lyophilized powder and reconstituted solution should homogeneity. Scyllatoxin, recombinant, is not amidated be stored at -20 °C or below. Repeated freezing and at the C-terminus, as is natural scyllatoxin. However, it thawing is not recommended. Storage in “frost-free” has been observed that C-terminal amidation has freezers is not recommended. If slight turbidity occurs negligible contribution both to activity and upon prolonged storage, clarify the solution by selectivity.The peptide concentration and identification centrifugation before use. Working dilution samples were determined by amino acid analysis. should be discarded if not used within 12 hours.

Scyllatoxin is a 31 amino acid toxin originally isolated Product Profile from the of the scorpion Leiurus quinquestriatus Application of 200 nM scyllatoxin, recombinant, hebraeus, and is a member of the a-KTx 5.1 scorpion inhibited KCa2.2 channels expressed in Xenopus oocytes. toxin family, having three disulfide bridges.1 Scyllatoxin appears to be selective for apamin-sensitive small- References conductance Ca2+-activated K+ channels (SK channels) 1. Chicchi, G.G., et al., Purification and with highest affinity towards Kca2.2. Scyllatoxin has characterization of a unique, potent inhibitor of proved to clearly discriminate SK channel subtypes. apamin binding from Leiurus quinquestriatus HEK 293 cell currents stably expressing hKca2.1 (hSK1) hebraeus venom. J. Biol. Chem., 263, 10192-10197 and Kca2.2 (hSK2) were blocked by scyllatoxin with IC50 (1988). 2 of 80 nM and 287 pM, respectively. The SK channel- 2. Strobaek, D., et al., Pharmacological mediated Afterhyperpolarising current (IAHP) of dorsal characterization of small-conductance 2+ + vagal neurons, assumed to be Kca2.3 (SK3)-mediated, Ca -activated K channels stably expressed in 3 was blocked by Scyllatoxin (20—30nM). Scyllatoxin is HEK 293 cells. Br. J. Pharmacol. 129, 991-999 thus a very useful compound to address the (2000). involvement of SK channel subtypes in functional 3. Pedarzani, P., et al., Molecular determinants of responses. Ca2+-dependent K+ channel function in rat dorsal vagal neurons. J. Physiol. 527, 283-290 (2000). Reagent Supplied as a lyophilized powder of unbuffered protein. MCT,PHC 11/05-1

Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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