KPNA5 Antibody Cat. No.: 6655

KPNA5 Antibody

Immunocytochemistry of KPNA5 in EL4 cells with KPNA5 Immunofluorescence of KPNA5 in EL4 cells with KPNA5 antibody at 5 μg/mL. antibody at 20 μg/mL.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (94%)

KPNA5 antibody was raised against an 18 synthetic peptide near the amino terminus of human KPNA5. IMMUNOGEN:

The immunogen is located within amino acids 50 - 100 of KPNA5.

TESTED APPLICATIONS: ELISA, ICC, IF, WB

September 24, 2021 1 https://www.prosci-inc.com/kpna5-antibody-6655.html KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

KPNA5 antibody is predicted to not cross-react with other alpha family SPECIFICITY: members.

POSITIVE CONTROL: 1) Cat. No. 1287 - EL4 Cell Lysate

2) Cat. No. 17-207 - EL4 Cell Slide

Properties

PURIFICATION: KPNA5 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

KPNA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: KPNA5

ALTERNATE NAMES: KPNA5 Antibody: SRP6, IPOA6, Importin subunit alpha-6, Karyopherin subunit alpha-5

ACCESSION NO.: CAH71948

PROTEIN GI NO.: 55664992

GENE ID: 3841

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 24, 2021 2 https://www.prosci-inc.com/kpna5-antibody-6655.html KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric complex consisting of alpha and beta subunits, is responsible for targeting with nuclear localization signals to the complex (NPC) by an energy requiring, Ran-dependent BACKGROUND: mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.

1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. REFERENCES: . Expr. 1997; 7:61-72.

2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem. 2003; 278: 51937-49

3) Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol. 1999; 19:7782-91.

ANTIBODIES FOR RESEARCH USE ONLY.

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