hConnexin 62/GJA10 VersaClone cDNA Catalog Number: RDC1106

Specifications: Description This shuttle vector contains the complete ORF for the of interest, Gene: hGJA10 along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: NP_115991 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 1644bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5

Shipping Ships at ambient temperature

Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated hConnexin 62/GJA10 freeze-thaw cycles.

cDNA Plasmid

PfoI GJA10 , BstAPI NdeI alpha 10, 62kDa [ Homo sapiens BbeI ZraI KasI BmgBI (human) ] AatII NarI HpaI SfoI HindIII EcoRV BmtI Also known as: CX62 ScaI NheI BbvCI NotI NsiI EagI BclI BtgI Summary: NcoI GJA10 is a member of the NmeAIII AMP BanII gene family. It is a component of BpmI BsrFI EcoNI gap junctions, which are membrane- BsaI spanning that assemble to AhdI form gap junction channels that RDC1106 facilitate the transfer of ions and 4378 bps small molecules between cells. The hGJA10 (1-543) PsiI specificity of the gap junction is determined by which connexin COLE1 proteins comprise the hemichannel. PasI GJA10 may be involved in tracer coupling between horizontal cells of PpuMI Bsu36I AscI the retina. Mutations in connexin BssHII BsgI Bst1107I are associated with human PciI XbaI SalI diseases including sensorineural SmaI XmaI deafness, a variety of skin disorders, EcoRI peripheral neuropathy and PspXI XhoI cardiovascular diseases. Eco47III

FOR RESEARCH USE ONLY

NOT FOR USE IN HUMANS

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> RDC1106 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atgggggact ggaacttatt gggtggcatc ctagaggaag ttcactccca 501 ctcaaccata gtggggaaaa tctggctgac catcctcttc atcttccgaa tgctggtact tcgtgtggct gctgaggatg tctgggatga tgaacagtca 601 gcatttgcct gcaacacccg gcagccaggt tgcaacaata tctgttatga tgatgcattc cctatctctt tgatcaggtt ctgggtttta cagatcatct 701 ttgtgtcttc tccttctttg gtctatatgg gccatgcact ttataggctc agggcctttg agaaagacag gcagaggaaa aagtcacacc ttagagccca 801 gatggagaat ccagatcttg acttggagga gcagcaaaga atagataggg aactgaggag gttagaggag cagaagagga tccataaagt ccctctgaaa 901 ggatgtctgc tgcgtactta tgtcttacac atcttgacca gatctgtgct ggaagtagga ttcatgatag gccaatatat tctctatggg tttcaaatgc 1001 acccccttta caaatgcact caacctcctt gccccaatgc ggtggattgc tttgtatcca ggcccactga gaagacaatt ttcatgcttt ttatgcacag 1101 cattgcagcc atttccttgt tactcaatat actggaaata tttcatctag gcatcagaaa aattatgagg acactttata agaaatccag cagtgagggc 1201 attgaggatg aaacaggccc tccattccat ttgaagaaat attctgtggc ccagcagtgt atgatttgct cttcattgcc tgaaagaatc tctccacttc 1301 aagctaacaa tcaacagcaa gtcattcgag ttaatgtgcc aaagtctaaa accatgtggc aaatcccaca gccaaggcaa cttgaagtag acccttccaa 1401 tgggaaaaag gactggtctg agaaggatca gcatagcgga cagctccatg ttcacagccc gtgtccctgg gctggcagtg ctggaaatca gcacctggga 1501 cagcaatcag accattcctc atttggcctg cagaatacaa tgtctcagtc ctggctaggt acaactacgg ctcctagaaa ctgtccatcc tttgcagtag 1601 gaacctggga gcagtcccag gacccagaac cctcaggtga gcctctcaca gatcttcata gtcactgcag agacagtgaa ggcagcatga gagagagtgg 1701 ggtctggata gacagatctc gcccaggcag tcgcaaggcc agctttctgt ccagattgtt gtctgaaaag cgacatctgc acagtgactc aggaagctct 1801 ggttctcgga atagctcctg cttggatttt cctcactggg aaaacagccc ctcacctctg ccttcagtca ctgggcacag aacatcaatg gtaagacagg 1901 cagccctacc gatcatggaa ctatcacaag agctgttcca ttctggatgc tttctttttc ctttctttct tcctggggtg tgtatgtatg tttgtgttga 2001 cagagaggca gatggagggg gagattattt atggagagat aaaattattc attcgataca ttcagttaaa ttcaattcat aaggcgcgcc agtatactct 2101 agagtcgaca cccggggaat tcctcgagcg ctcgtctcta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 2201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 2301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 2601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 2801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 2901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3101 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3401 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 3601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 3701 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 3801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 3901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4001 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4301 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc

> RDC1106 Translated Insert Sequence 1 mgdwnllggi leevhshsti vgkiwltilf ifrmlvlrva aedvwddeqs afacntrqpg cnnicyddaf pislirfwvl qiifvsspsl vymghalyrl 101 rafekdrqrk kshlraqmen pdldleeqqr idrelrrlee qkrihkvplk gcllrtyvlh iltrsvlevg fmigqyilyg fqmhplykct qppcpnavdc 201 fvsrptekti fmlfmhsiaa islllnilei fhlgirkimr tlykkssseg iedetgppfh lkkysvaqqc micsslperi splqannqqq virvnvpksk 301 tmwqipqprq levdpsngkk dwsekdqhsg qlhvhspcpw agsagnqhlg qqsdhssfgl qntmsqswlg tttaprncps favgtweqsq dpepsgeplt 401 dlhshcrdse gsmresgvwi drsrpgsrka sflsrllsek rhlhsdsgss gsrnsscldf phwenspspl psvtghrtsm vrqaalpime lsqelfhsgc 501 flfpfflpgv cmyvcvdrea dgggdylwrd kiihsihsvk fns

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