Nontuberculous Mycobacteria Insights on Taxonomy and Evolution
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A Putative Cystathionine Beta-Synthase Homolog of Mycolicibacterium Smegmatis Is Involved in De Novo Cysteine Biosynthesis
University of Arkansas, Fayetteville ScholarWorks@UARK Theses and Dissertations 5-2020 A Putative Cystathionine Beta-Synthase Homolog of Mycolicibacterium smegmatis is Involved in de novo Cysteine Biosynthesis Saroj Kumar Mahato University of Arkansas, Fayetteville Follow this and additional works at: https://scholarworks.uark.edu/etd Part of the Cell Biology Commons, Molecular Biology Commons, and the Pathogenic Microbiology Commons Citation Mahato, S. K. (2020). A Putative Cystathionine Beta-Synthase Homolog of Mycolicibacterium smegmatis is Involved in de novo Cysteine Biosynthesis. Theses and Dissertations Retrieved from https://scholarworks.uark.edu/etd/3639 This Thesis is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of ScholarWorks@UARK. For more information, please contact [email protected]. A Putative Cystathionine Beta-Synthase Homolog of Mycolicibacterium smegmatis is Involved in de novo Cysteine Biosynthesis A thesis submitted in partial fulfillment of the requirement for the degree of Master of Science in Cell and Molecular Biology by Saroj Kumar Mahato Purbanchal University Bachelor of Science in Biotechnology, 2016 May 2020 University of Arkansas This thesis is approved for recommendation to the Graduate Council. ___________________________________ Young Min Kwon, Ph.D. Thesis Director ___________________________________ ___________________________________ Suresh Thallapuranam, Ph.D. Inés Pinto, Ph.D. Committee Member Committee Member ABSTRACT Mycobacteria include serious pathogens of humans and animals. Mycolicibacterium smegmatis is a non-pathogenic model that is widely used to study core mycobacterial metabolism. This thesis explores mycobacterial pathways of cysteine biosynthesis by generating and study of genetic mutants of M. smegmatis. Published in vitro biochemical studies had revealed three independent routes to cysteine synthesis in mycobacteria involving separate homologs of cysteine synthase, namely CysK1, CysK2, and CysM. -
Cusumano-Et-Al-2017.Pdf
International Journal of Infectious Diseases 63 (2017) 1–6 Contents lists available at ScienceDirect International Journal of Infectious Diseases journal homepage: www.elsevier.com/locate/ijid Rapidly growing Mycobacterium infections after cosmetic surgery in medical tourists: the Bronx experience and a review of the literature a a b c b Lucas R. Cusumano , Vivy Tran , Aileen Tlamsa , Philip Chung , Robert Grossberg , b b, Gregory Weston , Uzma N. Sarwar * a Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York, USA b Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York, USA c Department of Pharmacy, Nebraska Medicine, Omaha, Nebraska, USA A R T I C L E I N F O A B S T R A C T Article history: Background: Medical tourism is increasingly popular for elective cosmetic surgical procedures. However, Received 10 May 2017 medical tourism has been accompanied by reports of post-surgical infections due to rapidly growing Received in revised form 22 July 2017 mycobacteria (RGM). The authors’ experience working with patients with RGM infections who have Accepted 26 July 2017 returned to the USA after traveling abroad for cosmetic surgical procedures is described here. Corresponding Editor: Eskild Petersen, Methods: Patients who developed RGM infections after undergoing cosmetic surgeries abroad and who ?Aarhus, Denmark presented at the Montefiore Medical Center (Bronx, New York, USA) between August 2015 and June 2016 were identified. A review of patient medical records was performed. Keywords: Results: Four patients who presented with culture-proven RGM infections at the sites of recent cosmetic Mycobacterium abscessus complex procedures were identified. -
Mycobacterium Chelonae Complex Bacteremia from a Post-Renal
Jpn. J. Infect. Dis., 63, 61-64, 2010 Short Communication Mycobacterium chelonae Complex Bacteremia from a Post-Renal Transplant Patient: Case Report and Literature Review Ali Mohammed Somily*, Awadh Raheel AL-Anazi1, Hanan Ahmed Babay, Abdulkarim Ibraheem AL-Aska1, Mugbil Ahmed AL-Hedaithy1, Waleed Khalid Al-Hamoudi1, Ahmad Amer Al Boukai2, Mohammed Sarwar Sabri, Sahar Isa AlThawadi3, and Abdelmageed Mohamed Kambal Department of Pathology, Microbiology Unit, 1Department of Medicine, and 2Department of Radiology, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh; and 3Microbiology Section, Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia (Received August 5, 2009. Accepted December 2, 2009) SUMMARY: In this report we present a case of a young lady with abdominal abscesses and septicemia caused by Mycobacterium chelonae complex. Identification of the organism and initiation of the appropriate antimicrobial therapy was delayed, resulting in significant morbidity and multiple hospital admissions. Gram staining of these organisms from blood culture can be easily overlooked or confused with either debris or diptheroids. We concluded that detection of Gram-positive rod colonies should prompt an acid-fast stain to distinguish diphtheroids from rapidly growing mycobacteria in immunosuppressed patients. Non-tuberculous Mycobacterium (NTM), which are rap- mal. Blood cultures were collected on the 11th, 14th, and idly growing mycobacteria, were -
S1 Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm
Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm Communities in a Chloraminated Drinking Water Distribution System C. Kimloi Gomez-Smith 1,2 , Timothy M. LaPara 1, 3, Raymond M. Hozalski 1,3* 1Department of Civil, Environmental, and Geo- Engineering, University of Minnesota, Minneapolis, Minnesota 55455 United States 2Water Resources Sciences Graduate Program, University of Minnesota, St. Paul, Minnesota 55108, United States 3BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States Pages: 9 Figures: 2 Tables: 3 Inquiries to: Raymond M. Hozalski, Department of Civil, Environmental, and Geo- Engineering, 500 Pillsbury Drive SE, Minneapolis, MN 554555, Tel: (612) 626-9650. Fax: (612) 626-7750. E-mail: [email protected] S1 Table S1. Reference sequences used in the newly created alignment and taxonomy databases for hsp65 Illumina sequencing. Sequences were obtained from the National Center for Biotechnology Information Genbank database. Accession Accession Organism name Organism name Number Number Arthrobacter ureafaciens DQ007457 Mycobacterium koreense JF271827 Corynebacterium afermentans EF107157 Mycobacterium kubicae AY373458 Mycobacterium abscessus JX154122 Mycobacterium kumamotonense JX154126 Mycobacterium aemonae AM902964 Mycobacterium kyorinense JN974461 Mycobacterium africanum AF547803 Mycobacterium lacticola HM030495 Mycobacterium agri AY438080 Mycobacterium lacticola HM030495 Mycobacterium aichiense AJ310218 Mycobacterium lacus AY438090 Mycobacterium aichiense AF547804 Mycobacterium -
Characteristics of Nontuberculous Mycobacteria from a Municipal Water Distribution System and Their Relevance to Human Infections
CHARACTERISTICS OF NONTUBERCULOUS MYCOBACTERIA FROM A MUNICIPAL WATER DISTRIBUTION SYSTEM AND THEIR RELEVANCE TO HUMAN INFECTIONS. Rachel Thomson MBBS FRACP Grad Dip (Clin Epi) A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy School of Biomedical Sciences Faculty of Health Queensland University of Technology 2013 Principal Supervisor: Adjunct Assoc Prof Megan Hargreaves (QUT) Associate Supervisors: Assoc Prof Flavia Huygens (QUT) i ii KEYWORDS Nontuberculous mycobacteria Water Distribution systems Biofilm Aerosols Genotyping Environmental organisms Rep-PCR iii iv ABSTRACT Nontuberculous mycobacteria (NTM) are environmental organisms associated with pulmonary and soft tissue infections in humans, and a variety of diseases in animals. There are over 150 different species of NTM; not all have been associated with disease. In Queensland, M. intracellulare predominates, followed by M. avium, M. abscessus, M. kansasii, and M. fortuitum as the most common species associated with lung disease. M. ulcerans, M. marinum, M. fortuitum and M. abscessus are the most common associated with soft tissue (both community acquired and nosocomial) infections. The environmental source of these pathogens has not been well defined. There is some evidence that water (either naturally occurring water sources or treated water for human consumption) may be a source of pathogenic NTM. The aims of this investigation were to 1) document the species of NTM that are resident in the Brisbane municipal water distribution system, then 2) to compare the strains of NTM found in water, with those found in human clinical samples collected from Queensland patients. This would then help to prove or disprove whether treated water is likely to be a source of pathogenic strains of NTM for at risk patients. -
Structural and Biochemical Characterizations of Three Potential Drug Targets from Pathogens
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 2020 Structural and Biochemical Characterizations of Three Potential Drug Targets from Pathogens LU LU ACTA UNIVERSITATIS UPSALIENSIS ISSN 1651-6214 ISBN 978-91-513-1148-7 UPPSALA urn:nbn:se:uu:diva-435815 2021 Dissertation presented at Uppsala University to be publicly examined in Room A1:111a, BMC, Husargatan 3, Uppsala, Friday, 16 April 2021 at 13:15 for the degree of Doctor of Philosophy. The examination will be conducted in English. Faculty examiner: Christian Cambillau. Abstract Lu, L. 2021. Structural and Biochemical Characterizations of Three Potential Drug Targets from Pathogens. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 2020. 91 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-1148-7. As antibiotic resistance of various pathogens emerged globally, the need for new effective drugs with novel modes of action became urgent. In this thesis, we focus on infectious diseases, e.g. tuberculosis, malaria, and nosocomial infections, and the corresponding causative pathogens, Mycobacterium tuberculosis, Plasmodium falciparum, and the Gram-negative ESKAPE pathogens that underlie so many healthcare-acquired diseases. Following the same- target-other-pathogen (STOP) strategy, we attempted to comprehensively explore the properties of three promising drug targets. Signal peptidase I (SPase I), existing both in Gram-negative and Gram-positive bacteria, as well as in parasites, is vital for cell viability, due to its critical role in signal peptide cleavage, thus, protein maturation, and secreted protein transport. Three factors, comprising essentiality, a unique mode of action, and easy accessibility, make it an attractive drug target. -
Mycobacteria of Veterinary Interest
Rev. salud pública. 12 sup (2): 67-70, 2010 Virulence and pathogenicity - Conferences 67 Poster Presentation Mycobacteria of veterinary interest Production and potency of PPDs Mycobacterium phlei and Mycobacterium fortuitum isolated soils from La Pampa-Argentina Amelia Bernardelli1, Bernardo Alonso2, Delia Oriani3 1 SENASA, Dirección de Laboratorio y Control Técnico(DILAB),Lab. de Referencia en Paratuberculosis y Tuberculosis Bovina de la OIE, Buenos Aires-Republica Argentina. 2 SENASA (DILAB). 3 Universidad Nacional de La Pampa, Facultad de Ciencias Veterinarias, Cátedra de Microbiología, Gral. Pico, La Pampa -Republica Argentina. The Non-Tuberculous Mycobacteria (NTM),whose habitat the environment has ac- quired importance in the last years because of the immunosupressed patients, in- fected HIV ,and also in develop countries that have managed to eradicated the bovine tuberculosis. Where it has been verified that certain NTM interfere in the diagnosis of tuberculosis when it is applied to the delayed hypersensitivity test with purified protein derivative (PPD) tuberculin from Mycobacterium bovis. In the works to field exists controversy about the relevance of these environmental mycobacteria when control and eradication of animal tuberculosis are applied.The production of PPDs from the isolated soils from the province of La Pampa and the verification of the possible crossed reaction with bovine tuberculin PPD, prescribed test for international trade. Two lots of PPDs corresponding of M. phlei and M. fortuitum were elaborated with a protein content of 1.5mg/mL both.Guinea pigs were sensitized with dead M. phlei, M. fortuitum and M. bovis.After 60 days the potency tests were made bioassay at the guinea pigs, using also like standard of reference bovine PPD,Lot.N°5 DILAB and employing a Latin square design. -
Identification of Different Subtypes of Rapid Growing Atypical
International Journal of Mycobacteriology xxx (2016) xxx– xxx Available at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/IJMYCO Identification of different subtypes of rapid growing Atypical Mycobacterium from water and soil sources: Using PCR-RFLP using hsp65 and rRNA 16s–23s genes Mohammad Varahram a, Parissa Farnia a,*, Shima Saif a, Mehran Marashian a, Poopak Farnia a,b, Jaladein Ghanavi a, Ali Akbar Velayati a a Mycobacteriology Research Centre (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran b Department of Biotechnology Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran ARTICLE INFO ABSTRACT Article history: Objective/Background: Nontuberculosis mycobacteria (NTM) are a diverse group of microor- Received 13 September 2016 ganisms that cause a variety of diseases in humans including skin, respiratory, and gas- Accepted 14 September 2016 trointestinal tract infection. Generally, NTM are classified into two categories: rapid Available online xxxx (<7 days) and slow growing (>7 days). In this study, we aimed to investigate NTM frequency and prevalence in environmental samples. Additionally, we tried to identify various sub- Keywords: types of isolated rapid growing mycobacteria (RGM). Iran Methods: Through a prospective descriptive cross-sectional study, water and soil samples Mycobacterium fortuitum were gathered from four neighboring towns around Tehran, the capital of Iran, at different 2 RGM geographic directions. Every 100 m of the studied areas gave one sample containing 6 g of Soil soil in 3–5 cm depth deposited in 50 mL sterile water as sampling media. After digestion Water and decontamination, DNA from culture-positive specimens (RGM) were extracted using phenol–chloroform methods. -
면역정상인에서 발생한 Mycobacterium Abscessus에 의한 척추골 수염 제동모・강철인・정지영・정혜민・조윤영・허경민・백경란 성균관대학교 의과대학 내과학교실
Case Report Infection & http://dx.doi.org/10.3947/ic.2012.44.6.530 Chemotherapy Infect Chemother 2012;44(6):530-534 pISSN 2093-2340 eISSN 2092-6448 면역정상인에서 발생한 Mycobacterium abscessus에 의한 척추골 수염 제동모・강철인・정지영・정혜민・조윤영・허경민・백경란 성균관대학교 의과대학 내과학교실 Vertebral Osteomyelitis caused by Mycobacterium Dongmo Je, Cheol-In Kang, Ji young Joung, Hyemin abscessus in an Immunocompetent Patient Jeong, Yoon Young Cho, Kyungmin Huh, and Kyong Ran Peck Vertebral osteomyelitis caused by nontuberculous mycobacteria (NTM) is rarely reported, especially in an immunocompetent host. NTM are usually not susceptible Department of Internal Medicine, Samsung Medical in vitro to antituberculous drugs, and appropriate antimicrobial therapy for Center, Sungkyunkwan University School of Medicine, treatment of NTM infection is based on susceptibility results, which vary between Seoul, Korea different NTM species; therefore, treatment of vertebral osteomyelitis caused by NTM is challenging. We report on the first case of vertebral osteomyelitis caused by M. abscessus in an otherwise healthy individual, confirmed by cultures of bone tissue obtained during surgery. Clinical cure was achieved with a combination of antimicrobial therapy and surgery. We also review previous reports of vertebral osteomyelitis caused by NTM. Key Words: Nontuberculous Mycobacteria, Mycobacterium abscessus, Vertebral Osteomyelitis, Immunocompetent Host This is an Open Access article distributed under the terms of the Creative Introduction Commons Attribution Non-Commercial License (http://creativecommons. org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work Nontuberculous mycobacteria (NTM) are free-living organisms that are is properly cited. ubiquitous in the environment. -
Piscine Mycobacteriosis
Piscine Importance The genus Mycobacterium contains more than 150 species, including the obligate Mycobacteriosis pathogens that cause tuberculosis in mammals as well as environmental saprophytes that occasionally cause opportunistic infections. At least 20 species are known to Fish Tuberculosis, cause mycobacteriosis in fish. They include Mycobacterium marinum, some of its close relatives (e.g., M. shottsii, M. pseudoshottsii), common environmental Piscine Tuberculosis, organisms such as M. fortuitum, M. chelonae, M. abscessus and M. gordonae, and Swimming Pool Granuloma, less well characterized species such as M. salmoniphilum and M. haemophilum, Fish Tank Granuloma, among others. Piscine mycobacteriosis, which has a range of outcomes from Fish Handler’s Disease, subclinical infection to death, affects a wide variety of freshwater and marine fish. It Fish Handler’s Nodules has often been reported from aquariums, research laboratories and fish farms, but outbreaks also occur in free-living fish. The same organisms sometimes affect other vertebrates including people. Human infections acquired from fish are most often Last Updated: November 2020 characterized by skin lesions of varying severity, which occasionally spread to underlying joints and tendons. Some lesions may be difficult to cure, especially in those who are immunocompromised. Etiology Mycobacteriosis is caused by members of the genus Mycobacterium, which are Gram-positive, acid fast, pleomorphic rods in the family Mycobacteriaceae and order Actinomycetales. This genus is traditionally divided into two groups: the members of the Mycobacterium tuberculosis complex (e.g., M. tuberculosis, M. bovis, M. caprae, M. pinnipedii), which cause tuberculosis in mammals, and the nontuberculous mycobacteria. The organisms in the latter group include environmental saprophytes, which sometimes cause opportunistic infections, and other species such as M. -
Health Monitoring for Your Zebrafish Colonies
Discover better insight Health Monitoring for your zebrafish colonies The clear choice for a comprehensive health monitoring program. BioResearch Your partner in discovery Contents Introduction . 3 Edwardsiella ictaluri. 4 Flavobacterium columnare . 5 Ichthyophthirius multifiliis. 6 Infectious spleen and kidney necrosis virus (ISKNV) . 7 Mycobacterium abscessus . 8 Mycobacterium chelonae . .9 Mycobacterium fortuitum. 10 Mycobacterium haemophilum. 11 Mycobacterium marinum. 12 Mycobacterium peregrinum . 13 Piscinoodinium pillulare . 14 Pleistophora hyphessobryconis . 15 Pseudocapillaria tomentosa . 16 Pseudoloma neurophilia . 17 Profiles and Pricing. 18 Specimen preparation and shipping . 20 Additional resources . 21 Introduction A growing number of researchers are choosing zebrafish as models for biomedical research because of the advantages zebrafish offer over other animal models for certain studies. First, their small size and ease of breeding make zebrafish relatively inexpensive to maintain, which allows researchers to perform experiments using zebrafish that would be cost prohibitive using larger animal models. Secondly, embryos are transparent, which allows easy visualization of cell and organ development and permits experimental manipulations involving DNA or mRNA injection, cell labeling and transplantation. Zebrafish are now commonly employed as models in a diverse range of bioresearch fields, such as immunology, infectious disease, cardiac and vascular disease research, chemical and drug toxicity studies, reproductive biology and cancer research to name a few. As with other vertebrate models used in research, undetected infections can alter, confound or invalidate experimental results. Therefore, it is important to develop and utilize a health monitoring program to detect infectious agents that may affect the animal and the research outcomes. IDEXX BioResearch has developed sensitive molecular diagnostic assays to improve health monitoring for zebrafish colonies. -
Accepted Manuscript
Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium Item Type Article Authors Tortoli, E.; Meehan, Conor J.; Grottola, A.; Fregni Serpini, J.; Fabio, A.; Trovato, A.; Pecorari, M.; Cirillo, D.M. Citation Tortoli E, Meehan CJ, Grottola A et al (2019) Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium. Infection, Genetics and Evolution. 75: 103983. Rights © 2019 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) Download date 29/09/2021 07:10:28 Link to Item http://hdl.handle.net/10454/17474 Accepted Manuscript Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium Enrico Tortoli, Conor J. Meehan, Antonella Grottola, Giulia Fregni Serpini, Anna Fabio, Alberto Trovato, Monica Pecorari, Daniela M. Cirillo PII: S1567-1348(19)30201-1 DOI: https://doi.org/10.1016/j.meegid.2019.103983 Article Number: 103983 Reference: MEEGID 103983 To appear in: Infection, Genetics and Evolution Received date: 13 June 2019 Revised date: 21 July 2019 Accepted date: 25 July 2019 Please cite this article as: E. Tortoli, C.J. Meehan, A. Grottola, et al., Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium, Infection, Genetics and Evolution, https://doi.org/10.1016/j.meegid.2019.103983 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript.