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Renxiao Wang, Ph Department of Cell Biology Harvard Medical School 240 Longwood Ave. Boston, MA 02115 [email protected] Junying Yuan, Ph.D. Tel: 617 432-4170 Research Regulation of apoptosis and inflammation by caspases. Interests Regulation of autophagy. The molecular mechanism of necroptosis, a programmed necrotic cell death pathway. Professional 2000-present: Professor of Cell Biology ,Harvard Medical School Experience 1997-2000:Associate Professor of Cell Biology,Harvard Medical School 1996-1997:Assistant Professor of Cell Biology,Harvard Medical School. 1992-1996:Assistant Professor of Medicine,Harvard Medical School 1990-1991:Instructor in Medicine,Harvard Medical School Education 1989-1996: Ph.D., Harvard University Major: Neuroscience Director: Prof. H. R. Horvitz, Academician Member of the American Academy of Arts and Sciences, The Nobel Prize winner 1982-1989: B.S., Fudan University, Shanghai, China Major: Biochemistry Honors and Fellow of the American Academy of Arts and Sciences (2007) Awards International Cell Death Society Award in Appreciation of Her Contribution to Research in the Field of Cell Death (2006) NIH Director’s Pioneer Award (2005) BBS Mentoring Award, Harvard Medical School(2003) Merit Award, National Institute of Aging (2002) Innovator Award for Breast Cancer Research(2002) SCBA Outstanding Young Investigator Award (1999) American Heart Established Investigator (1996-1999) Wilson S. Stone Memorial Award (1994) Ryan Fellowship(1985-1989) Professional Senior Editor of " Journal of Cell Biology " Associations Reviewing Editor of “Journal of Neuroscience” Associate Editor of " Genes to Cells " Executive Editor of “Chinese Science Bulletin” 1 Latest update: 02/10/2009 Member of the Editorial Board of " Current Biology " Founding Board member of Rosalind Franklin Society. number of American Association for Cancer Research number of Society of Chinese Bioscientists in America number of American Society for Cell Biology number of Society for Neuroscience number of American Society for Biochemistry and Molecular Biology h number of American Association for Advancement of Science Original Articles: Selected 1. Yuan J, Horvitz HR. The C. elegans genes ced-3 and ced-4 act cell-autonomou Publications cause programmed cell death. Dev. Biol. 1990; 138:33-41. 2. Yuan J, Finney M, Kim S, Horvitz HR. Tc4, a C. elegans transposon with an unusual foldback structure. Proc. Natl. Acad. Sci. USA 1991; 88:3334-3338 3. Yuan J, Horvitz HR. The C. elegans cell death gene ced-4 encodes a novel protein and is expressed during the period of extensive programmed cell death. Development 1992; 116:309-320 4. Yuan J, Shaham S, Ledoux S, Ellis HM, Horvitz HR. The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1b converting enzyme. Cell 1993; 75: 641-652. 5. Miura M, Zhu H, Hartwieg EA, Rotello R, Yuan J. Induction of apoptosis in fibroblasts by interleukin-1b converting enzyme, a mammalian homolog of C. elegans cell death gene ced-3. Cell 1993; 75: 653-660 6. Gagliardini V, Fernandez PA, Lee RKK, Drexler HCA, Rotello R, Fishman M, Yuan J. Prevention of vertebrate neuronal death by the crmA gene. Science 1994; 263: 826-828. 7. Rotello R, Fernandez PA, Yuan J. Anti-apogens and anti-engulfens: monoclonal antibodies reveal specific antigens on cells undergoing programmed cell death in developing chick embryos. Development 1994; 120: 1421-1431. 8. Fernandez PA, Rotello R, Rangini Z, Doupe A, Yuan J. Expression of a specific marker of avian programmed cell death in both apoptosis and necrosis. Proc. Natl. Acad. Sci. 1994; 91:8641-8645. 9. Wang L, Miura M, Bergeron L, Zhu H, Yuan J. Ich-1, an Ice/Ced-3 related gene, encodes both positive and negative regulators of programmed cell death. Cell 1994; 78:739-750. 10. Miura M, Friedlander RM, Yuan J. TNF-induced apoptosis is mediated by a CrmA-sensitive cell death pathway. Proc. Natl. Acad. Sci. USA PNAS. 1995; 92: 8318-8322. 11. Takashita N, Homma S, Rottello RJ, Fernandez PA, Yuan J, Oppenheim RW,Yaginuma H. Expression of apogens and engulfens during programmed cell death in the nervous system of the chick embryo. Arch Histol Cytol 1995; 58: 243-248 12. Li W, Fishman MC, Yuan J. Prevention of apoptosis in CNTF-dependent neuron a mutated ICE or viral protein CrmA but not by proto-oncogene Bcl-2. Cell Death Differ. 1996; 3:105-112. 13. Jung YK, Miura M, Yuan J. Suppression of interleukin-1b-converting enzyme-m cell death by insulin-like growth factor. J. Biol. Chem. 1996; 271: 5112-5117. 14. Friedlander RM, Gagliardini V, Rotello RJ, Yuan J. Functional role of endogenous IL-1 in apoptosis mediated by the ICE. J. Exp. Med. 1996; 184:717-724. 15. Wang S, Miura M, Jung YK, Zhu H, Gagliardini V, Shi L, Greenberg AH and 2 Latest update: 02/10/2009 Yuan J. Identification and Characterization of ICH-3, a Member of the ICE/Ced-3 Family and an Upstream Regulator of ICE. J. Biol. Chem. 1996; 271: 20580-20587. 16. Shi L, Chen G, Salvesen G, Quan L, Bergeron L, Miura M, Rotello RR, Miller DK, Li P, Seshadri T, Yuan J, Greenberg AH. Activation of an ICE- dependent apoptosis pathway by granzme B. Proc Natl Acad Sci. 1996; 93:11002-11007. 17. Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW, Yuan J. Human ICE/CED-3 protease nomenclature. Cell. 1996; 87: 171. 18. Cryns VL, Bergeron L, Zhu H, Li H, and Yuan J. Specific cleavage of - fodrin during Fas- and TNF-induced apoptosis is mediated by an ICE/Ced-3 family protease distinct from the PARP protease. J Biol Chem. 1996; 271:31277-31282. 19. Jung YK, Yuan J. Induction and suppression of ICE induced apoptosis by oncogenes. Oncogene, 1997; 14:1207-1214. 20. Hara H, Friedlander RM, Gagliardini V, Ayata C, Huang Z, Sasamata M, Yuan J and Moskowitz MA. Inhibition of ICE-Ced-3 family of poteases and ischemic/excitotoxic neuronal damage. Proc Natl Acad Sci U S A 1997; 94:2007-2012. 21. Friedlander RM, Gagliardini V, Hara H, MacDonald G, Greenberg AH, Moskowitz MA, and Yuan J. Inhibition of neuronal apoptosis in a transgenic mouse expressing a dominant negative mutant of ICE. J Exp Med. 1997; 185:933-940. 22. Johnson AL, Bridgham JT, Bergeron L, and Yuan J. Characterization of the avian ich-1 cDNA and expression of ich-1 long mRNA in the hen ovary. Gene. 1997; 192: 227-233. 23. Hara H, Fink K, Endres M, Friedlander RM, Gagliardini V, Yuan J, Moskowitz MA. Attenuation of transient focal cerebral ischemic injury in transgenic mice expressing a mutant ICE inhibitory protein. J Cereb Blood Flow Metab. 1997; 17: 370-375. 24. Friedlander RM, Gagliardini V, Wang J, Brown RH, Yuan J. Inhibition of ICE slows ALS in mice. Nature. 1997; 388: 31. 25. Li H, Bergeron L, Cryns V, Pasternack MS, Zhu H, Shi L, Greenberg AH and Yuan J. Activation of caspase-2 in apoptosis. J Biol Chem. 1997; 272: 21010-21017. 26. Haviv R, Lindenboim L, Li H, Yuan J, & Stein R. Need for caspases in apoptosis of trophic factor-deprived PC12 cells. J Neurosci Res. 1997; 50: 69- 80. 27. Cryns, VL, Byun Y, Rana A, Mellor| H, Lustig KL, Parker P, Avruch J, Kirschner MW, Yuan J. Specific Proteolysis of the Kinase PRK2 by Caspase- 3 during Apoptosis: Identification by a novel, small pool expression cloning strategy. J Biol Chem. 1997; 272:29449-29453. 28. Zhang Y, Center DM, Wu DMH, Cruikshank WW, Yuan J, Andrews DW, Kornfeld H. Processing and activation of pro-interleukin-16 by caspase-3. J Biol Chem. 1998; 273:1144-1149. 29. Wang S, Miura M, Jung Y-K, Zhu H, Li E, Yuan J. Murine caspase-11, an ICE interacting protease, is essential for the activation of ICE. Cell. 1998; 92:501-509. 30. Bergeron L, Perez GI, MacDonald G, Shi L, Sun Y, Jurisicova| A, Varmuza S, Latham KE, Flaws JA, Salter J, Hara H, Moskowitz MA, Li E, Greenberg A, Tilly JL, Yuan J. Defects in regulation of apoptosis in caspase-2-deficient mice. Genes & Dev. 1998; 12:1304-1314. 31. Weng LP, Yuan J, Yu Q. Overexpression of the transmembrane tyrosine phosphatase LAR activates the caspase pathway and induces apoptosis. Curr Biol. 1998; 8: 247-256. 32. Namura S, Zhu J, Fink K, Endres M, Srinivasan A, Tomaselli KJ, Yuan J, 3 Latest update: 02/10/2009 Moskowitz MA. Activation and cleavage of caspase-3 in apoptosis induced by experimental cerebral ischemia. J Neurosci. 1998; 18: 3659-3668. 33. Zhou BB, Li H, Yuan J, Kirschner MW. Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells. Proc Natl Acad Sci U S A. 1998; 95: 6785-6790. 34. Li H, Zhu H, Xu C-J, and Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. 1998; 94:491- 501. 35. Hilbi H, Moss JE, Hersh D, Chen Y, Arondel J, Banerjee S, Flavell RA, Yuan J, Sansonetti PJ, and Zychlinsky A. Shigella-induced apoptosis is dependent on caspase-1 Which binds to IpaB. J Biol Chem. 1998; 273:32895-32900. 36. Haviv R, Lindenboim L, Yuan J, Stein R. Need for caspase-2 in apoptosis of growth-factor-deprived PC12 cells. J Neurosci Res. 1998; 52:491-497. 37. Juo P, Kuo CJ, Yuan J, Blenis J. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr Biol. 1998; 8:1001-8 38. Fink K, Zhu J, Namura S, Shimizu-Sasamata M, Endres M, Ma J, Dalkara T, Yuan J, Moskowitz MA. Prolonged therapeutic window for ischemic brain damage caused by delayed caspase activation.
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