Katherine M. Graham, M.S.

New England Aquarium Central Wharf, Boston, Massachusetts 20110 Phone: 617-226-2237; Email: [email protected]

Professional Experience: 2015-Present Assistant Scientist Anderson Cabot Center for Ocean Life, New England Aquarium Boston, Massachusetts 2014-2015 Graduate Research Assistant Mississippi State University Research Lab, MS State University Starkville, Mississippi 2012-2014 Endocrine Technician Disney’s Animal Kingdom, Walt Disney World Lake Buena Vista, Florida 2011-2012 Reproductive Biology Intern Disney’s Animal Kingdom, Walt Disney World Lake Buena Vista, Florida 2010-2010 Behavioral Biology Summer Fellow San Diego Zoo Institute for Conservation Research San Diego, California 2008-2011 Junior Research Associate Oregon Zoo (in conjunction with the University of Portland) Portland, Oregon

Education: 2015 Master of Science: Animal Physiology) Mississippi State University (Starkville, Mississippi) Thesis: Conserving the Mississippi gopher frog (Lithobates sevosa) through the use of assisted reproductive technologies 2011 Bachelor of Science: Biology; Chemistry minor) University of Portland (Portland, Oregon) Summer study abroad program: London, England (2008)

Professional Activities & Committees: 2019 Plastics Task Force to evaluate plastic use & research areas at the New England Aquarium 2017-present New England Aquarium Institutional Animal Care and Use Committee (IACUC) 2017-present International Society of Wildlife (ISWE) Communications Subcommittee 2015-present New England Aquarium SeaStars Community Outreach Program 2018 New England Biolabs Molecular Biology Workshop 2017-2018 New England Aquarium Exhibit & Master Planning Committee 2009-2011 Resident Assistant, Mehling Hall, University of Portland 2009-2011 Chemistry Teaching Assistant, University of Portland

Professional Affiliations: 2018-present American Association of Zoos and Aquariums (AZA) 2017-present Society for Marine Mammalogy (SMM) 2012-present International Society of Wildlife Endocrinology (ISWE) 2015-2018 Phi Kappa Phi Honors Society 2015-2016 Gamma Sigma Delta Agriculture Honor Society Certifications & Technical Skills: • Extensive experience performing commercial and in-house enzyme immunoassay and radioimmunoassay techniques. • Fecal, serum, and baleen extraction techniques. • Use and maintenance of common immunoassay equipment, including: microplate readers, scintillation counters, gamma counters, freeze dryers, and centrifuges. • Experience with immunoassay plate loader robotics (Beckman-Coulter Biomek 3000). • Certification to handle radioactive materials. • Amphibian handling and husbandry. • Amphibian gamete collection (oocytes and sperm), sperm analysis (motility and SYBR 14 viability staining), and artificial fertilization techniques. • Ultrasound analysis of follicular development in frogs (Sonosite Titan and MicroMaxx systems). • Trained in molecular biology laboratory techniques including: DNA and RNA extraction and purification, PCR, gene cloning, gene editing using CRISPR/cas9, gel electrophoresis. • Training on informal education presentations in zoo and aquarium settings. • Experience with SoftMaxPro and Prism (assay curve-fitting software), SigmaPlot (graphing software), SAS and SPSS (statistical software), and Microsoft Suite.

Awards and Honors: 2017 International Society for Wildlife Endocrinology Conference Best Poster Award 2015 Gamma Sigma Delta Honor Society Graduate Award 2015 Mississippi State University Graduate Research Symposium: 2nd Place Oral presentation Life and Biomedical Sciences category 2011 Mehling Hall Student Leadership Award 2009-2011 Geraldine Marie Virnig Memorial Scholarship (academic scholarship) 2009 Murdock Charitable Trust Summer Scholar Award (research scholarship) 2007-2011 University of Portland President’s Scholarship (academic scholarship)

Publications: Peer Reviewed Papers: Rolland R.M., Graham, K.M., Stimmelmayr, R., Suydam, R.S., George, J.C. (2019). Chronic stress from fishing gear entanglement is recorded in baleen from a bowhead whale (Balaena mysticetus). Marine Science. Doi: 10.1111/mms.12596. Graham, K., Langhorne, C., Vance, C., Willard, S., Kouba, A. (2018). Ultrasound imaging improves hormone therapy strategies for induction of ovulation and in vitro fertilization in the endangered dusky gopher frog (Lithobates sevosa). Conservation Physiology. 6(1): coy020. Graham, K., Kouba, A., Langhorne, C., Marcec, R., Willard, S. (2016). Biological sex identification in the endangered dusky gopher frog (Lithobates sevosa): a comparison of body size measurements, secondary sex characteristics, ultrasound imaging, and urinary hormone analysis methods. Reproductive Biology and Endocrinology. 14:41; doi: 10.1186/s12958-016-0174-9. Graham, K., Wheaton, C.J., Mylniczenko, N., Burns, C., Bettinger, T. (2016). Examining factors that may influence accurate measurement of testosterone in sea turtles. Journal of Veterinary Diagnostic Investigation. 28(1):12-19. Abstracts: Graham, K., Fontenot, D., Plasse, C., Savage, A., Bettinger, T., Wheaton, C.J. Preliminary investigation of the use of non-invasive measurements of faecal immuno-reactive corticosterone in rufous fantails (Rhipidura rufifrons): A potential diagnostic tool for choice of best candidates for translocation. Third annual conference of the International Society of Wildlife Endocrinology. September 23-26, 2012. University of Veterinary Medicine, Vienna, Austria.

Presentations: Oral: “Use of Exogenous Hormone Treatments to Induce Ovulation in the Critically Endangered Mississippi Gopher Frog (Lithobates sevosa)”. Joint Meeting of Ichthyologists and Herpetologists. July 15-19, 2015. Reno, NV, USA. “Conserving through assisted reproductive technologies: use of exogenous hormone treatments to induce ovulation in the critically endangered Mississippi gopher frog (Lithobates sevosa)”. Graduate Student Research Symposium. March 21, 2015. Mississippi State University, Mississippi State, MS, USA. “Behavior and Development of an Asian Elephant (Elephas maximus) Calf: A Case Study”. Murdock Undergraduate Research Conference. October 30-31, 2009. Gonzaga University, Spokane, WA, USA. Posters: “Non-invasive fecal reproductive hormone assays for the northern fur seal (Callorhinus ursinus)”. 22nd Biennial conference on the Biology of Marine . October 23-27, 2017. Halifax, Nova Scotia, Canada. “Validation of non-invasive fecal reproductive hormone assays for the northern fur seal (Callorhinus ursinus)”. Sixth International Society of Wildlife Endocrinology conference. August 14-16, 2017. Disney’s Animal Kingdom, Orlando, FL, USA. “Effects of sample collection, processing, and storage treatment for validation of an enzyme immunoassay to monitor testosterone in circulation in male sea turtles (Chelonia mydas and Caretta caretta)”. Fourth annual conference of the International Society of Wildlife Endocrinology. October 14-16, 2013. Lincoln Park Zoo, Chicago, IL, USA. “Preliminary investigation of the use of non-invasive measurements of faecal immuno-reactive corticosterone in rufous fantails (Rhipidura rufifrons): A potential diagnostic tool for choice of best candidates for translocation”. Third annual conference of the International Society of Wildlife Endocrinology. September 23-26, 2012. University of Veterinary Medicine, Vienna, Austria.

Media, Blogs, & Public Lectures: • Radio: “Can Save A Species? Boston Has The World's Largest Collection Of Right Whale Poop” and “Everything You Didn't Know You Wanted To Know About Marine Mammal Poop”. WBUR: Boston’s NPR News Station (Jan 2019) • Blog: “The Secrets in Seal Scat”. New England Aquarium Blog: http://www.neaq.org/blog/the- secrets-in-seal-scat/ (Dec 2018) • Video: New England Aquarium Study on in Northern Fur Seals. Prepared for the IMATA (International Marine Animal Trainer’s Association) conference (Oct 2018). • Public lecture: “Using Feces to Study Species: How northern fur seal fecal samples are helping us learn about the species’ reproductive biology”. Part of broader lecture: “Seal Studies: What Aquarium Scientists Are Learning About Disease, Physiology, and Population Dynamics to Help Protect Seals”. New England Aquarium Lowell Lecture series (May 2018). • Lecture: “The ART of frog reproduction: Using assisted reproductive technologies (ART) to conserve amphibians”. New England Aquarium “Brown-Bag” lecture series (Mar 2016).

Grants Awarded (Lead PI) 2019 Fisheries and Oceans Canada: “Validation of hormone immunoassays for blubber in the NARW (Eubalaena glacialis)” 2016 The Eppley Foundation for Research: “Reproductive in the northern fur seal”