BIOTA-FAPESP PROGRAM (S values 50%are not shown. bootstraps; Numbers below are significance values for 1000 of16SrDNAgenesequences.algorithm analysis usingtheminimumevolution (ME) basedonadistance Petrolisthes andrelated groups, Dendrogram ofthecluster analysis for of species FAPESP Jan2011to2015 Dec Grant 2010/50188-8I Term: Caetano daCosta Rogério Fernando Zara, José Antonio Leão Castilho, researchers: Main Sciences andLiterature /University ofSãoPaulo (USP) PretoRibeirão ofPhilosophy, School Fernando Luis Mantellato Medina POPULATION DYNAMICS) BIOLOGY, MOLECULAR SPERMIOTAXONOMY, OF THE STATE OFSÃOPAULO SEACOASTAL (, BIODIVERSITY MULTIDISCIPLINARY CHARACTERIZATION : DECAPOD uc:Mneat ta.2011. Mantelatto etal. ource: Z o.Stud. ool. 50 : 372-384) on our efforts inthisproject. on ourefforts area to studiedandthismotivated be usto carry than c we are more found intheSãoPaulo coastline, ab Considering that of thereproductive system. ontheevolution aswell as, studies onphylogeny, tha out 50%ofthisdec iden accurate detailedtaxonomic revision and populational andreproductive dynamic) for (tax andecological analysesmorphological applications (mitochondrial andnuclearDNA), the optimization ofmoleculartechniques is mainobjective of theSãoPaulo coast.The andshrimps) lobsters, , hermit shrimps, ghost crustaceansof thedecapod (crabs, ofthebiodiversity the analysis ofsomeaspects studies onCrustacea taxonomy andphylogeny. b combination has ecological dynamic.This spermatophoresperm/ ultrastructureand and DNAlibr andphylogenetic task. in amultidisciplinary coastal environments andmarine the estuarine, c and geneticsequences asituation described 10% ofthisfaunahasitsspermiomorphology mor B isfarfrom morphology theDecapoda sperm onmolecularsystematicof knowledge and theamount outinnumerous studies, carried ultrastructurehasbeen Although thesperm t oast decapods crustaceousoast decapods inhabitants of een succ r has c azilian bio on nm,pyoey spermiotaxonomy, phylogeny, onomy, e tific This searches newsolutionsfor project The moleculargenesusedfor taxonomy than 650sp vinced that we have here apromising ompr a tion ofthebio essfully emplo omising thec iest,whichisestimated in diversity, ar y will be integratedwill be to the ce.Frisac,lessthan For instance, ecies. ap o d diversity ofSãoPaulo yed inseveral recent fauna issupp onduc tion ofaccur osedly a t e BIOTA-FAPESP PROGRAM C 2009. its sutur ofampulla(a)and Detail C, B, (pd). peduncle of spermatophore,showing ampulla(a)and General morphology A, of spermatophores. c (DNA-bar endorsedfor agenomiclibrary Paulo coast, up to date crustaceans ofdecapod thespecies of theSão the majorobjectives. essential willbe outside ofnational to boundaries complete fromstudies withspecimens otherBrazilian regions and compared area ofSãoPaulo withothersat the northern State. pr for microscopy transmission electron andscanning and sampleshave processed been Regarding spermiotaxonomy, andare stillincurrentthe Genbank analysis of phylogeny. new sequences have obtainedanddeposited been at Interms ofmolecularperspectives, ofouraims. directions w andgenera species groupskey ofsometaxaalldecapod the thesampling, during Inaddition, of snappingshrimp. andonenewspecies, includinganalienspecies, region, obtained at leastfive newrecords for ofdecapod SãoPaulo we have result, As apreliminary Comprida andIguape). Ilha the faunaofSãoPaulo region southern (Cananeia, i.e. area diversity, interms ofdecapod out inalessknown sampling expedition alongthecoast ofSãoPaulo was carried c TO DATE ANDPERSPECTIVES SUMMARY OFRESULTS available on-line. o dn,semoaooyadpplto yai,thefirst spermiotaxonomy andpopulation dynamic, oding, alcinus tibic ere obtained andhave already processed been inallthree ofile ofsomec ding) and a scientific collection ofreferenceding) andascientific collection withsamples .Cut Biol. Crust. J. Finally, the combination of all these efforts brought thecombination ofalltheseefforts Finally, comparative thehighcomplexity ofourresults, Due oaheeorgaso oeua hlgn,DNAbar To achieve ourgoalsonmolecularphylogeny, e line (sl). en . Sc (S 29 our anning electronic micrographsanning electronic ommer 466-475) : c e: Amadio &Mantelatto Amadio e: cial penaidean shrimps when shrimps cial penaidean p in differences interesting evidences pr our dynamic, population On analysis. results sp and sperm for both chosen system were r the male regions of diff epr opulation er eliminary t er erent ma o ms of duc t ophor tive e MAIN PUBLICATIONS Southwestern-Atlantic hermit Spermatophore and gonoporeofthe morphology 2010. Mantelatto FL. Fantucci MZ, Scelzo MA, Does 2011. IS. I,Wehrtmann Miranda Pileggi LG, Mantelatto FL, male reproductive system crab of thehermit ofthe Description 2009. Mantelatto FL. Amadio LM, &Francis/CRC Press. Florida:Taylor Raton, Boca 579. amazonicum tibicen Chapter 29. Molecular phylogeny Molecular ofthegenus Chapter 29. M 421-433. o form complex aspecies orare we dealingwithjust Achelous withreassignment of 1860, Stimpson, Paguridae). 1892)(Anomura, (Benedict, C variability oftheAmazon Riverprawn Genetic 2011. Mantelatto FL. Pileggi LG, Vergamini FG, F F 29 C flman +55-16-3602-3656 SP–Brasil Preto, CEP 14040-901 – Ribeirão 3.900 Avenida Bandeirantes, deBiologia Departamento Preto /Universidade deSãoPaulo (USP) se aculdade deF er ne widelydistributed species? r nrb Zool. ontrib. neat L olsR cuatC,Fle L 2009. Felder DL. CD, Schubart R, Robles antelatto FL, ustac v : er 466-475. nando L In: al A t el@usp Dcpd:aoua diogenidae). anomura: (: a sus1:Decapod Phylogenetics ean Issues 18: M eHa,13 Bahua otnde.P.567- Pp. ). 1833(Brachyura: Haan, De mer ar Petrolisthes armatus tin, ic 8 uis M Dcpd,Crda Palaemonidae). Caridea, (Decapoda, .br 0 an sp .. rnal ..&Fle,DL (eds), D.L. &Felder, K.A. Crandall, J.W., ilosofia, : 67-83. edina M ecies of C iências eL P antelatto or Aoua Porcellanidae) (Anomura, tunus Z o.Stud. ool. etras deRibeirão Pagurus exilis t .tumidulus C. Macrobrachium o ol Stud. Zool. the .Cut Biol. Crust. J. 5 0 : Calcinus 372-384. Cronius 49 and : .