Yarrowia Parophonii Fungal Planet Description Sheets 289

288 Persoonia – Volume 39, 2017

Yarrowia parophonii Fungal Planet description sheets 289

Fungal Planet 628 – 20 December 2017 Yarrowia parophonii Gouliamova, R.A. Dimitrov, Guéorguiev, M.T. Sm., & M. Groenew., sp. nov. Etymology. Named after the insect host Parophonus hirsutulus from which Yarrowia oslonensis (Knutsen et al.) Gouliamova, R.A. Dimi the ex-type strain was isolated. trov, M.T. Sm., & M. Groenew., comb. nov. — MycoBank Classiﬁcation — Dipodascaceae, Saccharomycetales, Sac- MB819718 charomycetes. Basionym. Candida osloniensis Knutsen et al., Int. J. Syst. Evol. Microbiol. 57: 2426. 2007. After 5 d at 25 °C in yeast malt extract (YM) broth, cells are ovoid to globose, 2–6 × 3–9 µm in size. Vegetative reproduction Yarrowia alimentaria (Knutsen et al.) Gouliamova, R.A. Dimi is by multilateral budding. On glucose peptone yeast extract trov, M.T. Sm., & M. Groenew., comb. nov. — MycoBank agar (GPYA) and 5 % malt agar (MA) after 7 d at 25 °C streak MB819719 colonies are cream, butyrous, smooth, convex and with an en- Basionym. Candida alimentaria Knutsen et al., Int. J. Syst. Evol. Microbiol. tire margin. True hyphae are formed. After 1–2 wk of incubation 57: 2426. 2007. at 25 °C 1–2 hat-shaped ascospores were observed on YM Yarrowia hollandica (Knutsen et al.) Gouliamova, R.A. Dimi agar medium (Yarrow 1998). For full physiological test results trov, M.T. Sm., & M. Groenew., comb. nov. — MycoBank see MycoBank MB819716. MB819720 Typus. Bulgaria, from the gut of Parophonus hirsutulus (Carabidae), Basionym. Candida hollandica Knutsen et al., Int. J. Syst. Evol. Microbiol. 9 Aug. 2009, D. Gouliamova (holotype D189, ex-type cultures NBIMCC 57: 2426. 2007. 8889 = CBS 12427 preserved in metabolically inactive state, D1/D2 do- mains of LSU rDNA and ITS (ITS1+2) sequences in GenBank JQ026370.2, Yarrowia phangngaensis (Limtong et al.) Gouliamova, R.A. JQ026371.2, MycoBank MB819716). Dimitrov, M.T. Sm., & M. Groenew., comb. nov. — MycoBank Additional materials examined. Bulgaria, from the gut of Parophonus MB819721 hirsutulus (Carabidae), 9 Aug. 2009, D. Gouliamova, 83Y (NBIMCC 8888 Basionym. Candida phangngensis Limtong et al., Int. J. Syst. Evol. = CBS 12462), 25L (NBIMCC 8900 = CBS 12468), 30L (NBIMCC 8902 = Microbiol. 58: 515. 2008. CBS 12466), BH1 (NBIMCC 9804 = CBS 12471), KL1 (NBIMCC 8903 = CBS 12465) and 29L (NBIMCC 8901 = CBS 12441), LSU sequences GenBank KC810944, KY457253–KY457257; ITS sequences GenBank KC810951, Phylogenetic analysis of the combined ITS and LSU rDNA T KY457248–KY457252, respectively. sequences of Yarrowia paraphonii D189 and related species using a neighbour-joining analysis (Kimura two-parameter Notes — The phylogenetic analysis of the combined ITS model; MEGA v. 6). and LSU rDNA sequence alignment showed that the ex-type The following combinations were invalidly published, citing the strain clusters with C. oslonensis (96 % identity; 679 conserved MycoBank number of the basionym for the new combination nt., 1 subst., 1 gap in ITS region; 16 subst. in LSU rDNA). The (Crous et al. 2016b), and are herewith validated: additional strains examined show 100 % sequence similarity to one another, with exception of strains 25L and BH1 which Priceomyces fermenticarens (Van der Walt & Baker) Goulia- showed 99 % similarity in ITS (3 nt. subst.). The new strains, mova, R.A. Dimitrov, M.T. Sm., M.M. Stoilova-Disheva & D189, 83Y, 25l, 30L and KL1 can be distinguished from C. os- M. Groenew., comb. nov. — MycoBank MB818692 lonensis (CBS 10146T) based on assimilation proﬁles of twelve Basionym. Candida fermenticarens Van der Walt & Baker, Bothalia 12: compounds (details in MycoBank MB819716). The strains are 561. 1978. able to assimilate D-ribose (+/w/-), D-mannitol (+/w/-), gento- biose (+/w/-), Tween 40 (w), creatine (w) and can grow without Priceomyces northwykensis (R.S. Rao et al.) Gouliamova, vitamins (w), biotine and thiamine, pyridoxine and thiamine. R.A. Dimitrov, M.T. Sm., M.M. Stoilova-Disheva & M. Groe- The strains cannot assimilate L-sorbose, salicin and D-glucono new., comb. nov. — MycoBank MB818693 1.5 lactone. Hydrolysis of arbutin is negative. The strains were Basionym. Candida northwykensis R.S. Rao et al., Curr. Microbiol. 63: provisionally labelled mating type + (29L, 30L, KL1, 83Y, D189) 115. 2011. and mating type – (25L, BH1) (Wickerham et al. 1970, Knutsen et al. 2007, Groenewald & Smith 2013). Based on our results 100 Yarrowia oslonensis CBS 10146T AM279265/AM268477 we propose new combinations in the genus Yarrowia for the 62 Yarrowia parophonii D189T JQ026371/JQ026370 60 following Candida species. Yarrowia keelungensis CBS 11062T EF621566/EF621561 Yarrowia porcina NCAIM Y02100T KF649296/KF649289 76 67 Yarrowia yakushimensis CBS 10252A AM279260/AM268472 Yarrowia galli (G. Péter et al.) Gouliamova, R.A. Dimitrov, M.T. Yarrowia lipolytica NRRL YB-423T FJ153223/U40080 96 50 Sm. & M. Groenew., comb. nov. — MycoBank MB819717 95 Yarrowia deformans CBS 2071T AM279254/EF405984 Yarrowia divulgata CBS 11013T KF425323/EU194451 85 Basionym. Candida galli G. Péter et al., Antonie Van Leeuwenhoek 86: Yarrowia galli NCAIMY 01486T EU343806/AY346454 Yarrowia bubula NCAIMY 01998T KF649301/JN256212 107. 2004. 100 78 Yarrowia phangngaensis CBS 10407T NR 111357/AB304772 100 Yarrowia hollandica CBS 4855T AM279270/AM268482 Colour illustrations. The Kidney Lake in Rila mountains, Bulgaria; Paro Yarrowia alimentaria CBS 10151T AM279269/AM268481 Candida hispaniensis CBS 9996T AM279259/AY789654 phonus hirsutulus (photo credit Aleš Sedláček, http://www.hmyzfoto.cz); 100 Trigonopsis variabilis NRRL Y-1579T KY105763/JQ689074 T morphology of cells; true hyphae of Y. paraphonii D189 in YM broth (scale 100 Lipomyces starkeyi Y11557T AB614120/U45824 bars = 10 μm); cluster of asci, some with hat-shaped ascospores formed in Dipodascopsis anomalia NRRL Y-7931T KY103362/JQ689073 crosses between strains D189T and 25L (scale bar = 5 μm). 0.05

Dilnora E. Gouliamova, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, G. Bonchev 26, Sofia 1113, Bulgaria; e-mail: [email protected] Roumen A. Dimitrov, Sofia University ‘St. Kliment Ohridski’, 5 James Bourchier Blvd., Sofia 1164, Bulgaria; e-mail: [email protected] Borislav V. Guéorguiev, National Museum of Natural History, 1 Tsar Osvoboditel Blvd., Sofia 1000, Bulgaria; e-mail: [email protected] Maudy Th. Smith & Marizeth Groenewald, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected]